MD14G1073900.v1.1

Protein of unknown function (DUF563)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0002314
Physical Location & Seq
Forward (+)
8199795 .. 8208527
8733 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1073900.v1.1.491

Sequence Viewer

Length: 1455 bp
ATGGAGTTTTCAAACTCTGGTTTCTACAAAAGAACAAGGCCCAAGCTCCTCACTTTCGTCTTCCTCTCTCTCCTTTCTGCCTTGGTGCTCATGATATGGTTGTATACGCTCCCTTGGGTTCTTCAATCGCCAACGGTACGAACTATACTTTGTGATAGGAGCAATTTTCGATCAGATGTTTGTATCCTGAAAGGAAATGTAAGAACACACTCCGGATCCTCTTCCATCTTTGTCTACAGGTCCAGAGATAAAAGTAATTTCACATTCTATCTTTCGAGCATCGTCGAGGAAAATGAAGAGGAAAACGGGGAATGGCTTCAACACGAAAAGATCAAACCGTATACTCGGAAATGGGAACCAAGTACCATGGCCACCGTCACTGAATTAGACCTCATTGCAAAGAAAGACGATACTCTAGGGATGCAACATCAGTGTGACGTCCAACATGATGTTCCGGCGGTGTTCTTCTCAACGGCTGGCTATACTGGCAATGCTTATCATGAGTTCAACGACGGGATTATACCACTGTACATTACTTCTCAAGAACTGAGAAAGAAGGTTGTGTTTGTCATTCTTGATTTTCATGACTGGTGGCTTATGAAGTACGGAGACATTCTTTCACAACTATCAGACTATCCAGTAATAGATTTCAGTGCAGATACGAGAACCCATTGCTTTCCTGAAGCCATTGTCGGTTTGAGAATTCACGACGAGCTCACTGTGGATCCTTCGCTGATGGAGGGACATAGGAGCATTTTCGACTTTCAATATCTTCTAGACAGGGCGTACAGGCCTCGAATTATAGGTCTAATTAATGAACAAGAAGCAGAAGAGAAGCTTTCTGTTTCTGTGTCTCCAGCATCGGTAGGATCTTTAAAAATCAAGAAAAAACTACATGAAGCACATCAATTAGAGAGGCCTAAACTGGTGATCGTATCTAGAAACGGATCAAGAGCAATAACCAATGAGGACTTGTTGGTGGAAATGGCTGAGCAAATTGGGTTTGAAGTTGAAGTTCTGAGACCTGATTCGAGGACCGAGTTGGCAAAGATTTATTGGGTTCTTAATTCGAGTGATGTCGTGATAGGGGTTCACGGTGCCGCCATGACACATTTTCTGTTCATGAGACCTGGCTCTGTGTTGATCCAAGTGATTCCTCTTGGAACTAGTTGGGCAGCAGAGGCATACTTTGGGGAACCTGCAAGAAAGCTTGATTTAAACTACATTGGCTACAAAATTCTTCCCACAGAGAGCTCGTTGTACGACAAGTATGGCAAAGATGATCCTGTTCTTAAAAATCCAGCTAGTGTAACCAAAAAAGGTTGGCAACACACAAAGGAGATTTACCTTGAAGGTCAAACTGTGAGACTACACCTCACAAGATTTAAGAAGCAGTTGCTTGGTGCTTATGACTACACCACTGAAAGAATGACTGAGCATTTCGAACAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
DUF563

Protein Analysis

485

Amino Acids

55.7

Weight (kDa)

6.17

Isoelectric Point (pI)

33.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 288 - 382 3.3e-15 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 441
Acc36I ACCTGC 1 cut(s) 1207
AccB1I GGYRCC 1 cut(s) 1097
AccI GTMKAC 3 cut(s) 104, 234, 341
AccIII TCCGGA 1 cut(s) 212
AciI CCGC 2 cut(s) 458, 1101
AclWI GGATC 8 cut(s) 210, 223, 719, 732, 877, 955, 1138, 1277
AcoI YGGCCR 1 cut(s) 369
AcsI RAATTY 2 cut(s) 702, 1236
AcuI CTGAAG 1 cut(s) 702
AcyI GRCGYC 1 cut(s) 438
AfaI GTAC 6 cut(s) 138, 364, 530, 605, 788, 1262
AgsI TTSAA 8 cut(s) 12, 125, 320, 508, 767, 1007, 1013, 1352
AhlI ACTAGT 1 cut(s) 1166
AjnI CCWGG 1 cut(s) 1129
AjuI GAANNNNNNNTTGG 2 cut(s) 435, 467
AluBI AGCT 6 cut(s) 46, 715, 838, 1210, 1254, 1304
AluI AGCT 6 cut(s) 46, 715, 838, 1210, 1254, 1304
Alw21I GWGCWC 3 cut(s) 90, 717, 1256
Alw26I GTCTC 5 cut(s) 603, 858, 1015, 1120, 1360
AlwI GGATC 8 cut(s) 210, 223, 719, 732, 877, 955, 1138, 1277
Aor13HI TCCGGA 1 cut(s) 212
AoxI GGCC 4 cut(s) 38, 369, 791, 917
ApeKI GCWGC 1 cut(s) 1175
ApoI RAATTY 2 cut(s) 702, 1236
ArsI GACNNNNNNTTYG 2 cut(s) 790, 822
AseI ATTAAT 1 cut(s) 813
Asp700I GAANNNNTTC 1 cut(s) 315
AspS9I GGNCC 3 cut(s) 39, 240, 1035
AsuHPI GGTGA 1 cut(s) 940
AsuII TTCGAA 1 cut(s) 1443
AvaII GGWCC 2 cut(s) 240, 1035
BalI TGGCCA 1 cut(s) 371
BamHI GGATCC 2 cut(s) 215, 724
BanI GGYRCC 1 cut(s) 1097
BanII GRGCYC 2 cut(s) 717, 1256
BbsI GAAGAC 1 cut(s) 52
Bbv12I GWGCWC 3 cut(s) 90, 717, 1256
BbvI GCAGC 1 cut(s) 1187
BccI CCATC 2 cut(s) 233, 730
BceAI ACGGC 1 cut(s) 489
BciT130I CCWGG 1 cut(s) 1131
BciVI GTATCC 1 cut(s) 194
BcoDI GTCTC 5 cut(s) 603, 858, 1015, 1120, 1360
BcuI ACTAGT 1 cut(s) 1166
BfaI CTAG 5 cut(s) 416, 776, 939, 1167, 1305
BfmI CTRYAG 1 cut(s) 235
BfuAI ACCTGC 1 cut(s) 1207
BfuI GTATCC 1 cut(s) 194
BisI GCNGC 2 cut(s) 1101, 1176
BlpI GCTNAGC 1 cut(s) 990
BlsI GCNGC 2 cut(s) 1102, 1177
Bme1390I CCNGG 1 cut(s) 1131
Bme18I GGWCC 2 cut(s) 240, 1035
BmgT120I GGNCC 3 cut(s) 39, 240, 1035
BmiI GGNNCC 5 cut(s) 217, 357, 726, 1099, 1197
BmrFI CCNGG 1 cut(s) 1131
BmsI GCATC 3 cut(s) 288, 411, 869
BpiI GAAGAC 1 cut(s) 52
BpmI CTGGAG 1 cut(s) 840
Bpu1102I GCTNAGC 1 cut(s) 990
Bpu14I TTCGAA 1 cut(s) 1443
BpuEI CTTGAG 1 cut(s) 525
BsaHI GRCGYC 1 cut(s) 438
BsaI GGTCTC 2 cut(s) 1015, 1120
BsaJI CCNNGG 3 cut(s) 81, 113, 366
BsaWI WCCGGW 1 cut(s) 212
Bse1I ACTGG 4 cut(s) 490, 593, 638, 930
Bse3DI GCAATG 3 cut(s) 393, 496, 670
BseAI TCCGGA 1 cut(s) 212
BseBI CCWGG 1 cut(s) 1131
BseDI CCNNGG 3 cut(s) 81, 113, 366
BseGI GGATG 1 cut(s) 426
BseMI GCAATG 3 cut(s) 393, 496, 670
BseMII CTCAG 4 cut(s) 539, 981, 1010, 1425
BseNI ACTGG 4 cut(s) 490, 593, 638, 930
BseRI GAGGAG 1 cut(s) 38
BseXI GCAGC 1 cut(s) 1187
BsgI GTGCAG 1 cut(s) 675
BshFI GGCC 4 cut(s) 40, 371, 793, 919
BshNI GGYRCC 1 cut(s) 1097
BsiHKAI GWGCWC 3 cut(s) 90, 717, 1256
BsiSI CCGG 2 cut(s) 213, 455
BslFI GGGAC 1 cut(s) 756
BsmAI GTCTC 5 cut(s) 603, 858, 1015, 1120, 1360
BsmFI GGGAC 1 cut(s) 756
BsnI GGCC 4 cut(s) 40, 371, 793, 919
Bso31I GGTCTC 2 cut(s) 1015, 1120
Bsp119I TTCGAA 1 cut(s) 1443
Bsp1286I GDGCHC 3 cut(s) 90, 717, 1256
Bsp13I TCCGGA 1 cut(s) 212
Bsp1407I TGTACA 1 cut(s) 528
Bsp143I GATC 9 cut(s) 170, 215, 330, 724, 869, 930, 947, 1143, 1282
Bsp1720I GCTNAGC 1 cut(s) 990
Bsp19I CCATGG 1 cut(s) 366
BspACI CCGC 2 cut(s) 458, 1101
BspANI GGCC 4 cut(s) 40, 371, 793, 919
BspCNI CTCAG 4 cut(s) 540, 982, 1011, 1426
BspEI TCCGGA 1 cut(s) 212
BspHI TCATGA 4 cut(s) 90, 499, 583, 1122
BspLI GGNNCC 5 cut(s) 217, 357, 726, 1099, 1197
BspMI ACCTGC 1 cut(s) 1207
BspPI GGATC 8 cut(s) 210, 223, 719, 732, 877, 955, 1138, 1277
BspT104I TTCGAA 1 cut(s) 1443
BspT107I GGYRCC 1 cut(s) 1097
BspTNI GGTCTC 2 cut(s) 1015, 1120
BsrDI GCAATG 3 cut(s) 393, 496, 670
BsrGI TGTACA 1 cut(s) 528
BsrI ACTGG 4 cut(s) 490, 593, 638, 930
BssECI CCNNGG 3 cut(s) 81, 113, 366
BssMI GATC 9 cut(s) 170, 215, 330, 724, 869, 930, 947, 1143, 1282
BssNAI GTATAC 2 cut(s) 105, 342
BssNI GRCGYC 1 cut(s) 438
BssT1I CCWWGG 3 cut(s) 81, 113, 366
Bst1107I GTATAC 2 cut(s) 105, 342
Bst2UI CCWGG 1 cut(s) 1131
Bst4CI ACNGT 7 cut(s) 136, 339, 376, 528, 721, 1097, 1363
Bst6I CTCTTC 3 cut(s) 226, 291, 825
BstACI GRCGYC 1 cut(s) 438
BstAUI TGTACA 1 cut(s) 528
BstBI TTCGAA 1 cut(s) 1443
BstC8I GCNNGC 1 cut(s) 478
BstDEI CTNAG 4 cut(s) 548, 990, 1019, 1434
BstDSI CCRYGG 1 cut(s) 366
BstF5I GGATG 1 cut(s) 426
BstKTI GATC 9 cut(s) 173, 218, 333, 727, 872, 933, 950, 1146, 1285
BstMAI GTCTC 5 cut(s) 603, 858, 1015, 1120, 1360
BstMBI GATC 9 cut(s) 170, 215, 330, 724, 869, 930, 947, 1143, 1282
BstMWI GCNNNNNNNGC 2 cut(s) 486, 1181
BstNI CCWGG 1 cut(s) 1131
BstSCI CCNGG 1 cut(s) 1129
BstSFI CTRYAG 1 cut(s) 235
BstV1I GCAGC 1 cut(s) 1187
BstV2I GAAGAC 1 cut(s) 52
BstX2I RGATCY 3 cut(s) 215, 724, 869
BstYI RGATCY 3 cut(s) 215, 724, 869
BstZ17I GTATAC 2 cut(s) 105, 342
BsuI GTATCC 1 cut(s) 194
BsuRI GGCC 4 cut(s) 40, 371, 793, 919
BtgI CCRYGG 1 cut(s) 366
BtsCI GGATG 1 cut(s) 426
BtsIMutI CAGTG 6 cut(s) 378, 437, 524, 658, 717, 1419
BveI ACCTGC 1 cut(s) 1207
Cac8I GCNNGC 1 cut(s) 478
CciI TCATGA 4 cut(s) 90, 499, 583, 1122
Cfr13I GGNCC 3 cut(s) 39, 240, 1035
Csp6I GTAC 6 cut(s) 137, 363, 529, 604, 787, 1261
CviAII CATG 8 cut(s) 91, 367, 446, 500, 584, 896, 1105, 1123
CviQI GTAC 6 cut(s) 137, 363, 529, 604, 787, 1261
DdeI CTNAG 4 cut(s) 548, 990, 1019, 1434
DpnI GATC 9 cut(s) 172, 217, 332, 726, 871, 932, 949, 1145, 1284
DpnII GATC 9 cut(s) 170, 215, 330, 724, 869, 930, 947, 1143, 1282
DraI TTTAAA 2 cut(s) 876, 1218
EaeI YGGCCR 1 cut(s) 369
Eam1104I CTCTTC 3 cut(s) 226, 291, 825
EarI CTCTTC 3 cut(s) 226, 291, 825
Ecl136II GAGCTC 2 cut(s) 715, 1254
Eco130I CCWWGG 3 cut(s) 81, 113, 366
Eco147I AGGCCT 2 cut(s) 793, 919
Eco24I GRGCYC 2 cut(s) 717, 1256
Eco31I GGTCTC 2 cut(s) 1015, 1120
Eco47I GGWCC 2 cut(s) 240, 1035
Eco53kI GAGCTC 2 cut(s) 715, 1254
Eco57I CTGAAG 1 cut(s) 702
EcoICRI GAGCTC 2 cut(s) 715, 1254
EcoRI GAATTC 1 cut(s) 702
EcoRII CCWGG 1 cut(s) 1129
EcoT14I CCWWGG 3 cut(s) 81, 113, 366
EcoT38I GRGCYC 2 cut(s) 717, 1256
ErhI CCWWGG 3 cut(s) 81, 113, 366
FaeI CATG 8 cut(s) 94, 370, 449, 503, 587, 899, 1108, 1126
FalI AAGNNNNNCTT 2 cut(s) 822, 854
FaqI GGGAC 1 cut(s) 756
FatI CATG 8 cut(s) 90, 366, 445, 499, 583, 895, 1104, 1122
FblI GTMKAC 3 cut(s) 104, 234, 341
Fnu4HI GCNGC 2 cut(s) 1101, 1176
FokI GGATG 1 cut(s) 433
FriOI GRGCYC 2 cut(s) 717, 1256
Fsp4HI GCNGC 2 cut(s) 1101, 1176
FspBI CTAG 5 cut(s) 416, 776, 939, 1167, 1305
GluI GCNGC 2 cut(s) 1101, 1176
GsuI CTGGAG 1 cut(s) 840
HaeIII GGCC 4 cut(s) 40, 371, 793, 919
HapII CCGG 2 cut(s) 213, 455
Hin1I GRCGYC 1 cut(s) 438
Hin1II CATG 8 cut(s) 94, 370, 449, 503, 587, 899, 1108, 1126
HindIII AAGCTT 2 cut(s) 836, 1208
HinfI GANTC 2 cut(s) 1028, 1153
HpaII CCGG 2 cut(s) 213, 455
HphI GGTGA 1 cut(s) 940
Hpy166II GTNNAC 4 cut(s) 105, 235, 342, 1093
Hpy188I TCNGA 4 cut(s) 175, 348, 631, 1020
Hpy8I GTNNAC 4 cut(s) 105, 235, 342, 1093
Hpy99I CGWCG 3 cut(s) 287, 515, 713
HpyAV CCTTC 3 cut(s) 550, 738, 1346
HpyCH4III ACNGT 7 cut(s) 136, 339, 376, 528, 721, 1097, 1363
HpyCH4IV ACGT 1 cut(s) 438
HpyCH4V TGCA 4 cut(s) 398, 424, 656, 1202
HpyF10VI GCNNNNNNNGC 2 cut(s) 486, 1181
HpyF3I CTNAG 4 cut(s) 548, 990, 1019, 1434
HpySE526I ACGT 1 cut(s) 438
Hsp92I GRCGYC 1 cut(s) 438
Hsp92II CATG 8 cut(s) 94, 370, 449, 503, 587, 899, 1108, 1126
Kpn2I TCCGGA 1 cut(s) 212
Kzo9I GATC 9 cut(s) 170, 215, 330, 724, 869, 930, 947, 1143, 1282
LmnI GCTCC 4 cut(s) 51, 114, 159, 750
Lsp1109I GCAGC 1 cut(s) 1187
LweI GCATC 3 cut(s) 288, 411, 869
MaeI CTAG 5 cut(s) 416, 776, 939, 1167, 1305
MaeII ACGT 1 cut(s) 438
MaeIII GTNAC 3 cut(s) 376, 434, 1309
MalI GATC 9 cut(s) 172, 217, 332, 726, 871, 932, 949, 1145, 1284
MboI GATC 9 cut(s) 170, 215, 330, 724, 869, 930, 947, 1143, 1282
MboII GAAGA 8 cut(s) 52, 113, 213, 308, 457, 764, 842, 1232
MflI RGATCY 3 cut(s) 215, 724, 869
MhlI GDGCHC 3 cut(s) 90, 717, 1256
MlsI TGGCCA 1 cut(s) 371
MluNI TGGCCA 1 cut(s) 371
MmeI TCCRAC 1 cut(s) 466
Mox20I TGGCCA 1 cut(s) 371
MroI TCCGGA 1 cut(s) 212
MroXI GAANNNNTTC 1 cut(s) 315
MscI TGGCCA 1 cut(s) 371
MseI TTAA 6 cut(s) 813, 875, 1065, 1217, 1293, 1386
MslI CAYNNNNRTG 1 cut(s) 432
Msp20I TGGCCA 1 cut(s) 371
MspI CCGG 2 cut(s) 213, 455
MspR9I CCNGG 1 cut(s) 1131
MvaI CCWGG 1 cut(s) 1131
MwoI GCNNNNNNNGC 2 cut(s) 486, 1181
NcoI CCATGG 1 cut(s) 366
NdeII GATC 9 cut(s) 170, 215, 330, 724, 869, 930, 947, 1143, 1282
NlaIII CATG 8 cut(s) 94, 370, 449, 503, 587, 899, 1108, 1126
NlaIV GGNNCC 5 cut(s) 217, 357, 726, 1099, 1197
NmuCI GTSAC 2 cut(s) 376, 434
NspV TTCGAA 1 cut(s) 1443
PagI TCATGA 4 cut(s) 90, 499, 583, 1122
PceI AGGCCT 2 cut(s) 793, 919
PdmI GAANNNNTTC 1 cut(s) 315
PfeI GAWTC 2 cut(s) 1028, 1153
PkrI GCNGC 2 cut(s) 1102, 1177
PshBI ATTAAT 1 cut(s) 813
Psp124BI GAGCTC 2 cut(s) 717, 1256
Psp6I CCWGG 1 cut(s) 1129
PspGI CCWGG 1 cut(s) 1129
PspN4I GGNNCC 5 cut(s) 217, 357, 726, 1099, 1197
PspPI GGNCC 3 cut(s) 39, 240, 1035
PsuI RGATCY 3 cut(s) 215, 724, 869
RsaI GTAC 6 cut(s) 138, 364, 530, 605, 788, 1262
RsaNI GTAC 6 cut(s) 137, 363, 529, 604, 787, 1261
RseI CAYNNNNRTG 1 cut(s) 432
SacI GAGCTC 2 cut(s) 717, 1256
SaqAI TTAA 6 cut(s) 813, 875, 1065, 1217, 1293, 1386
SatI GCNGC 2 cut(s) 1101, 1176
Sau3AI GATC 9 cut(s) 170, 215, 330, 724, 869, 930, 947, 1143, 1282
Sau96I GGNCC 3 cut(s) 39, 240, 1035
ScrFI CCNGG 1 cut(s) 1131
SduI GDGCHC 3 cut(s) 90, 717, 1256
SfaNI GCATC 3 cut(s) 288, 411, 869
SfcI CTRYAG 1 cut(s) 235
SfuI TTCGAA 1 cut(s) 1443
SinI GGWCC 2 cut(s) 240, 1035
SmiMI CAYNNNNRTG 1 cut(s) 432
SmlI CTYRAG 1 cut(s) 540
SmoI CTYRAG 1 cut(s) 540
SpeI ACTAGT 1 cut(s) 1166
SseBI AGGCCT 2 cut(s) 793, 919
SsiI CCGC 2 cut(s) 458, 1101
SspMI CTAG 5 cut(s) 416, 776, 939, 1167, 1305
SstI GAGCTC 2 cut(s) 717, 1256
StuI AGGCCT 2 cut(s) 793, 919
StyD4I CCNGG 1 cut(s) 1129
StyI CCWWGG 3 cut(s) 81, 113, 366
TaaI ACNGT 7 cut(s) 136, 339, 376, 528, 721, 1097, 1363
TaiI ACGT 1 cut(s) 441
TaqI TCGA 8 cut(s) 169, 275, 285, 759, 796, 1031, 1070, 1443
TaqII GACCGA 1 cut(s) 1052
TatI WGTACW 1 cut(s) 528
TauI GCSGC 1 cut(s) 1103
TfiI GAWTC 2 cut(s) 1028, 1153
Tru1I TTAA 6 cut(s) 813, 875, 1065, 1217, 1293, 1386
Tru9I TTAA 6 cut(s) 813, 875, 1065, 1217, 1293, 1386
TscAI CASTG 6 cut(s) 385, 437, 531, 658, 724, 1426
TseFI GTSAC 2 cut(s) 376, 434
TseI GCWGC 1 cut(s) 1175
Tsp45I GTSAC 2 cut(s) 376, 434
TspDTI ATGAA 6 cut(s) 309, 572, 614, 831, 912, 1111
TspGWI ACGGA 2 cut(s) 621, 960
TspRI CASTG 6 cut(s) 385, 437, 531, 658, 724, 1426
VpaK11BI GGWCC 2 cut(s) 240, 1035
VspI ATTAAT 1 cut(s) 813
XapI RAATTY 2 cut(s) 702, 1236
XbaI TCTAGA 2 cut(s) 775, 938
XmiI GTMKAC 3 cut(s) 104, 234, 341
XmnI GAANNNNTTC 1 cut(s) 315
XspI CTAG 5 cut(s) 416, 776, 939, 1167, 1305
ZraI GACGTC 1 cut(s) 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.