MD12G1075900.v1.1

Protein of unknown function (DUF563)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002314
Physical Location & Seq
Forward (+)
9193507 .. 9196114
2608 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1075900.v1.1.491

Sequence Viewer

Length: 1617 bp
ATGGGGCGGTATCAGCGGCATCACCACCACCAGTGGAGAAAAGTTGACAAGCCTTGTGCAGCTGCAGAGGAAGACGAAGACTCACAGACCACTACCTTTCTAATGGAGTTTGCAAACTCTGGTTATTATTACAAGAGAACAAGTCCCAAGCTTCTCTATCTCCTCTTCATCTCCTTCCTCTCTTGCAGCTTCATCTTAGCCCCTCATCTTTTCAGCTCCAACACCACTTTCTCTCTCCTATATTCAACACCAATGGAGGATATGAAAGTAAATGTTCCCTTGTGTTCTTCAATCTCTAATGGAACTATATGTTGCGATCGAACCAGTATTCGTTCAGATATTTGTGTAATGAAAGGGGATGTAAGAACACACTGTGCTTCCTCCACCATCTTTCTCTACCGATCTAAAGAAGGAAGTAGCTTCCAAAATCATGTTTCTGATGTTGTTGAGGAAAATGAAGAAGATGAAACCGAGGAACTCGGGCACACAAAGGTCAAACCGTATACACGGAAATGGGAAACAAGTGTCATGGACACCATTGATGAATTACAGCTCGTTGCGAAGAAAGACACTCTGGGGATGCACCACCGGTGTGATGTCCAACATGATGTTCCGGCAGTGTTTTTCTCAACCGGGGGGTATACTGGTAATGTCTATCACGAATTCAATGATGGGATTATGCCGTTGTTCATTACTTCCCAGCGTTTCAACAAGAAGGTTGTGTTTGTCATTCTCGAGTACCATAATTGGTGGGTGACGAAATATGGAGACATTGTTTCTCAACTATCAGATTATCAACCCATAGATTTTAGTGGAGAAACAAGAACTCATTGCTTCCCTGAGGTCACCGTTGGTCTGAGGATTCACGACGAGCTCACTGTGGATTCCTCACTGATGGAGGGAAATGTGAGCATTGTTGACTTTCGAAATCTTCTAGACCGAGCCTACTGGCCTCGAATTAGAAGTCTAATTCAAGACGAGGAACGGGAAGCACCGGAGAAGCATTCTGCGTCTCTGGCATCTGAAAGCTCTTTCGAACCTGAGAGTGAAGTGCAAGAAGATCAATTCAAGAAGCCTAAGCTGGTCATCATATCTCGAAATGGATCAAGAGCGATAACCAATGAGAATTTCTTGGTTAGAATGGCGGAGCAAATTGGGTTTGAAGTTAATGTTTTGAGACCTGACCGCACGACAGAGTTAGCAAAGATTTATCGGGCCCTTAATGCGAGTGATGTCATGATCGGGGTTCATGGCGCTGCCATGACACATTTTATGTTCATGAGGCCTGGGTCCGTGTTCATCCAAGTTGTTCCCCTAGGAACTGAGTGGGCAGCGGAGGAATACTATGGGGAACCTGCAAGGAAGCTCGGGTTGAAATACATCGGCTACCAGATTCTTACTCGAGAGAGCTCGTTGTACGCCAAATACCGTAAGGATGATCCTGTTCTTAGGGATCCACAGAGTGTAACCAAAATGGGATGGGAGTACACAAAGAAGATCTATCTTGATGGCCAAACTGTGAGATTAGACCTCCGAAGATTTCGGAAGCGATTGGTTCGTGCTTACGATTACATCTTCCACAGCTTGAACAATCGCCCCCATCTTCCATCACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
DUF563

Protein Analysis

539

Amino Acids

62.22

Weight (kDa)

6.22

Isoelectric Point (pI)

36.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 346 - 434 3.3e-14 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1363
AccI GTMKAC 2 cut(s) 503, 641
AciI CCGC 5 cut(s) 7, 16, 1145, 1186, 1334
AclWI GGATC 4 cut(s) 1111, 1433, 1448, 1461
AcoI YGGCCR 1 cut(s) 1510
AcsI RAATTY 2 cut(s) 662, 1126
AdeI CACNNNGTG 2 cut(s) 374, 1463
AfaI GTAC 3 cut(s) 740, 1418, 1487
AgeI ACCGGT 1 cut(s) 588
AgsI TTSAA 9 cut(s) 246, 291, 667, 709, 974, 1069, 1163, 1375, 1588
AjnI CCWGG 1 cut(s) 1285
AjuI GAANNNNNNNTTGG 6 cut(s) 212, 244, 594, 626, 1112, 1144
AleI CACNNNNGTG 1 cut(s) 591
Alw21I GWGCWC 2 cut(s) 876, 1412
Alw26I GTCTC 3 cut(s) 762, 1017, 1171
AlwI GGATC 4 cut(s) 1111, 1433, 1448, 1461
Ama87I CYCGRG 4 cut(s) 479, 734, 1367, 1401
AoxI GGCC 4 cut(s) 950, 1215, 1283, 1510
ApaI GGGCCC 1 cut(s) 1219
ApeKI GCWGC 5 cut(s) 59, 62, 186, 1256, 1331
ApoI RAATTY 2 cut(s) 662, 1126
ArsI GACNNNNNNTTYG 2 cut(s) 949, 981
AsiGI ACCGGT 1 cut(s) 588
AspA2I CCTAGG 1 cut(s) 1315
AspLEI GCGC 1 cut(s) 1256
AspS9I GGNCC 3 cut(s) 1215, 1216, 1290
AsuC2I CCSGG 1 cut(s) 634
AsuHPI GGTGA 3 cut(s) 14, 766, 838
AsuII TTCGAA 2 cut(s) 925, 1035
AvaI CYCGRG 4 cut(s) 479, 734, 1367, 1401
AvaII GGWCC 1 cut(s) 1290
AvrII CCTAGG 1 cut(s) 1315
AxyI CCTNAGG 1 cut(s) 840
BaeGI GKGCMC 2 cut(s) 486, 1219
BalI TGGCCA 1 cut(s) 1512
BamHI GGATCC 1 cut(s) 1453
BanII GRGCYC 3 cut(s) 876, 1219, 1412
BbsI GAAGAC 2 cut(s) 78, 84
Bbv12I GWGCWC 2 cut(s) 876, 1412
BbvI GCAGC 5 cut(s) 49, 71, 198, 1243, 1343
BccI CCATC 7 cut(s) 395, 665, 889, 1473, 1502, 1608, 1615
BceAI ACGGC 1 cut(s) 667
BciT130I CCWGG 1 cut(s) 1287
BcnI CCSGG 1 cut(s) 634
BcoDI GTCTC 3 cut(s) 762, 1017, 1171
BfaI CTAG 2 cut(s) 935, 1316
BfmI CTRYAG 1 cut(s) 63
BfoI RGCGCY 1 cut(s) 1257
BfuAI ACCTGC 1 cut(s) 1363
BglII AGATCT 1 cut(s) 1497
BisI GCNGC 6 cut(s) 17, 60, 63, 187, 1257, 1332
BlnI CCTAGG 1 cut(s) 1315
BlsI GCNGC 6 cut(s) 18, 61, 64, 188, 1258, 1333
Bme1390I CCNGG 2 cut(s) 634, 1287
Bme18I GGWCC 1 cut(s) 1290
BmeT110I CYCGRG 4 cut(s) 479, 734, 1367, 1401
BmgT120I GGNCC 3 cut(s) 1215, 1216, 1290
BmiI GGNNCC 4 cut(s) 1217, 1291, 1353, 1455
BmrFI CCNGG 2 cut(s) 634, 1287
BmsI GCATC 3 cut(s) 28, 570, 1028
BpiI GAAGAC 2 cut(s) 78, 84
Bpu10I CCTNAGC 1 cut(s) 1077
Bpu14I TTCGAA 2 cut(s) 925, 1035
BpuMI CCSGG 1 cut(s) 634
BsaI GGTCTC 1 cut(s) 1171
BsaJI CCNNGG 4 cut(s) 471, 633, 1286, 1315
BsaWI WCCGGW 2 cut(s) 588, 994
BsaXI ACNNNNNCTCC 2 cut(s) 759, 789
Bse118I RCCGGY 1 cut(s) 588
Bse1I ACTGG 4 cut(s) 31, 324, 649, 953
Bse21I CCTNAGG 1 cut(s) 840
Bse3DI GCAATG 1 cut(s) 829
BseBI CCWGG 1 cut(s) 1287
BseDI CCNNGG 4 cut(s) 471, 633, 1286, 1315
BseGI GGATG 5 cut(s) 364, 585, 1299, 1441, 1484
BseMI GCAATG 1 cut(s) 829
BseMII CTCAG 4 cut(s) 831, 848, 1032, 1314
BseNI ACTGG 4 cut(s) 31, 324, 649, 953
BseRI GAGGAG 1 cut(s) 152
BseSI GKGCMC 2 cut(s) 486, 1219
BseXI GCAGC 5 cut(s) 49, 71, 198, 1243, 1343
BseYI CCCAGC 1 cut(s) 699
BsgI GTGCAG 1 cut(s) 78
Bsh1285I CGRYCG 1 cut(s) 319
BshFI GGCC 4 cut(s) 952, 1217, 1285, 1512
BshTI ACCGGT 1 cut(s) 588
BsiEI CGRYCG 1 cut(s) 319
BsiHKAI GWGCWC 2 cut(s) 876, 1412
BsiHKCI CYCGRG 4 cut(s) 479, 734, 1367, 1401
BsiSI CCGG 4 cut(s) 589, 614, 633, 995
BslFI GGGAC 1 cut(s) 129
BsmAI GTCTC 3 cut(s) 762, 1017, 1171
BsmBI CGTCTC 1 cut(s) 1017
BsmFI GGGAC 1 cut(s) 129
BsmI GAATGC 1 cut(s) 1003
BsnI GGCC 4 cut(s) 952, 1217, 1285, 1512
Bso31I GGTCTC 1 cut(s) 1171
BsoBI CYCGRG 4 cut(s) 479, 734, 1367, 1401
Bsp119I TTCGAA 2 cut(s) 925, 1035
Bsp120I GGGCCC 1 cut(s) 1215
Bsp1286I GDGCHC 4 cut(s) 486, 876, 1219, 1412
Bsp143I GATC 8 cut(s) 316, 401, 1060, 1103, 1239, 1438, 1453, 1497
BspACI CCGC 5 cut(s) 7, 16, 1145, 1186, 1334
BspANI GGCC 4 cut(s) 952, 1217, 1285, 1512
BspCNI CTCAG 4 cut(s) 832, 849, 1033, 1315
BspHI TCATGA 2 cut(s) 1236, 1278
BspLI GGNNCC 4 cut(s) 1217, 1291, 1353, 1455
BspMAI CTGCAG 1 cut(s) 67
BspMI ACCTGC 1 cut(s) 1363
BspPI GGATC 4 cut(s) 1111, 1433, 1448, 1461
BspT104I TTCGAA 2 cut(s) 925, 1035
BspTNI GGTCTC 1 cut(s) 1171
BsrDI GCAATG 1 cut(s) 829
BsrFI RCCGGY 1 cut(s) 588
BsrI ACTGG 4 cut(s) 31, 324, 649, 953
BssAI RCCGGY 1 cut(s) 588
BssECI CCNNGG 4 cut(s) 471, 633, 1286, 1315
BssMI GATC 8 cut(s) 316, 401, 1060, 1103, 1239, 1438, 1453, 1497
BssNAI GTATAC 2 cut(s) 504, 642
BssT1I CCWWGG 1 cut(s) 1315
Bst1107I GTATAC 2 cut(s) 504, 642
Bst2UI CCWGG 1 cut(s) 1287
Bst4CI ACNGT 7 cut(s) 374, 501, 850, 880, 1430, 1519, 1614
Bst6I CTCTTC 1 cut(s) 170
BstBI TTCGAA 2 cut(s) 925, 1035
BstDEI CTNAG 7 cut(s) 196, 840, 857, 1041, 1077, 1323, 1448
BstEII GGTNACC 1 cut(s) 844
BstF5I GGATG 5 cut(s) 364, 585, 1299, 1441, 1484
BstH2I RGCGCY 1 cut(s) 1257
BstHHI GCGC 1 cut(s) 1256
BstKTI GATC 8 cut(s) 319, 404, 1063, 1106, 1242, 1441, 1456, 1500
BstMAI GTCTC 3 cut(s) 762, 1017, 1171
BstMBI GATC 8 cut(s) 316, 401, 1060, 1103, 1239, 1438, 1453, 1497
BstMCI CGRYCG 1 cut(s) 319
BstMWI GCNNNNNNNGC 3 cut(s) 13, 1016, 1223
BstNI CCWGG 1 cut(s) 1287
BstPI GGTNACC 1 cut(s) 844
BstSCI CCNGG 2 cut(s) 632, 1285
BstSFI CTRYAG 1 cut(s) 63
BstSLI GKGCMC 2 cut(s) 486, 1219
BstV1I GCAGC 5 cut(s) 49, 71, 198, 1243, 1343
BstV2I GAAGAC 2 cut(s) 78, 84
BstX2I RGATCY 2 cut(s) 1453, 1497
BstYI RGATCY 2 cut(s) 1453, 1497
BstZ17I GTATAC 2 cut(s) 504, 642
Bsu36I CCTNAGG 1 cut(s) 840
BsuRI GGCC 4 cut(s) 952, 1217, 1285, 1512
BtsCI GGATG 5 cut(s) 364, 585, 1299, 1441, 1484
BtsI GCAGTG 1 cut(s) 624
BtsIMutI CAGTG 5 cut(s) 38, 370, 624, 876, 890
BveI ACCTGC 1 cut(s) 1363
CciI TCATGA 2 cut(s) 1236, 1278
CfoI GCGC 1 cut(s) 1256
Cfr10I RCCGGY 1 cut(s) 588
Cfr13I GGNCC 3 cut(s) 1215, 1216, 1290
CseI GACGC 1 cut(s) 999
Csp6I GTAC 3 cut(s) 739, 1417, 1486
CspAI ACCGGT 1 cut(s) 588
CviAII CATG 7 cut(s) 431, 529, 605, 1237, 1250, 1261, 1279
CviQI GTAC 3 cut(s) 739, 1417, 1486
DdeI CTNAG 7 cut(s) 196, 840, 857, 1041, 1077, 1323, 1448
DpnI GATC 8 cut(s) 318, 403, 1062, 1105, 1241, 1440, 1455, 1499
DpnII GATC 8 cut(s) 316, 401, 1060, 1103, 1239, 1438, 1453, 1497
DraIII CACNNNGTG 2 cut(s) 374, 1463
EaeI YGGCCR 1 cut(s) 1510
Eam1104I CTCTTC 1 cut(s) 170
EarI CTCTTC 1 cut(s) 170
EciI GGCGGA 1 cut(s) 1160
Ecl136II GAGCTC 2 cut(s) 874, 1410
Eco130I CCWWGG 1 cut(s) 1315
Eco147I AGGCCT 1 cut(s) 1285
Eco24I GRGCYC 3 cut(s) 876, 1219, 1412
Eco31I GGTCTC 1 cut(s) 1171
Eco47I GGWCC 1 cut(s) 1290
Eco53kI GAGCTC 2 cut(s) 874, 1410
Eco81I CCTNAGG 1 cut(s) 840
Eco88I CYCGRG 4 cut(s) 479, 734, 1367, 1401
Eco91I GGTNACC 1 cut(s) 844
EcoICRI GAGCTC 2 cut(s) 874, 1410
EcoO109I RGGNCCY 1 cut(s) 1216
EcoO65I GGTNACC 1 cut(s) 844
EcoRI GAATTC 1 cut(s) 662
EcoRII CCWGG 1 cut(s) 1285
EcoT14I CCWWGG 1 cut(s) 1315
EcoT38I GRGCYC 3 cut(s) 876, 1219, 1412
ErhI CCWWGG 1 cut(s) 1315
Esp3I CGTCTC 1 cut(s) 1017
FaeI CATG 7 cut(s) 434, 532, 608, 1240, 1253, 1264, 1282
FaqI GGGAC 1 cut(s) 129
FatI CATG 7 cut(s) 430, 528, 604, 1236, 1249, 1260, 1278
FblI GTMKAC 2 cut(s) 503, 641
Fnu4HI GCNGC 6 cut(s) 17, 60, 63, 187, 1257, 1332
FokI GGATG 5 cut(s) 371, 592, 1286, 1448, 1491
FriOI GRGCYC 3 cut(s) 876, 1219, 1412
Fsp4HI GCNGC 6 cut(s) 17, 60, 63, 187, 1257, 1332
FspBI CTAG 2 cut(s) 935, 1316
GlaI GCGC 1 cut(s) 1255
GluI GCNGC 6 cut(s) 17, 60, 63, 187, 1257, 1332
GsaI CCCAGC 1 cut(s) 703
HaeII RGCGCY 1 cut(s) 1257
HaeIII GGCC 4 cut(s) 952, 1217, 1285, 1512
HapII CCGG 4 cut(s) 589, 614, 633, 995
HgaI GACGC 1 cut(s) 999
HhaI GCGC 1 cut(s) 1256
Hin1II CATG 7 cut(s) 434, 532, 608, 1240, 1253, 1264, 1282
Hin6I GCGC 1 cut(s) 1254
HinP1I GCGC 1 cut(s) 1254
HincII GTYRAC 2 cut(s) 46, 919
HindII GTYRAC 2 cut(s) 46, 919
HindIII AAGCTT 1 cut(s) 149
HinfI GANTC 4 cut(s) 80, 862, 884, 1393
HpaII CCGG 4 cut(s) 589, 614, 633, 995
HphI GGTGA 3 cut(s) 14, 766, 838
Hpy166II GTNNAC 5 cut(s) 46, 504, 642, 919, 1488
Hpy188I TCNGA 7 cut(s) 337, 439, 790, 858, 1024, 1535, 1545
Hpy8I GTNNAC 5 cut(s) 46, 504, 642, 919, 1488
Hpy99I CGWCG 1 cut(s) 872
HpyAV CCTTC 3 cut(s) 184, 404, 709
HpyCH4III ACNGT 7 cut(s) 374, 501, 850, 880, 1430, 1519, 1614
HpyCH4V TGCA 7 cut(s) 59, 65, 113, 186, 583, 1054, 1358
HpyF10VI GCNNNNNNNGC 3 cut(s) 13, 1016, 1223
HpyF3I CTNAG 7 cut(s) 196, 840, 857, 1041, 1077, 1323, 1448
Hsp92II CATG 7 cut(s) 434, 532, 608, 1240, 1253, 1264, 1282
HspAI GCGC 1 cut(s) 1254
Kzo9I GATC 8 cut(s) 316, 401, 1060, 1103, 1239, 1438, 1453, 1497
LmnI GCTCC 2 cut(s) 221, 1147
Lsp1109I GCAGC 5 cut(s) 49, 71, 198, 1243, 1343
LweI GCATC 3 cut(s) 28, 570, 1028
MaeI CTAG 2 cut(s) 935, 1316
MaeIII GTNAC 3 cut(s) 754, 844, 1465
MalI GATC 8 cut(s) 318, 403, 1062, 1105, 1241, 1440, 1455, 1499
MboI GATC 8 cut(s) 316, 401, 1060, 1103, 1239, 1438, 1453, 1497
MflI RGATCY 2 cut(s) 1453, 1497
MhlI GDGCHC 4 cut(s) 486, 876, 1219, 1412
MlsI TGGCCA 1 cut(s) 1512
MluCI AATT 8 cut(s) 545, 662, 745, 957, 969, 1064, 1126, 1152
MluNI TGGCCA 1 cut(s) 1512
MlyI GAGTC 1 cut(s) 74
MmeI TCCRAC 2 cut(s) 243, 625
Mox20I TGGCCA 1 cut(s) 1512
MscI TGGCCA 1 cut(s) 1512
MseI TTAA 2 cut(s) 1167, 1221
MslI CAYNNNNRTG 2 cut(s) 511, 591
Msp20I TGGCCA 1 cut(s) 1512
MspA1I CMGCKG 3 cut(s) 16, 62, 1334
MspI CCGG 4 cut(s) 589, 614, 633, 995
MspR9I CCNGG 2 cut(s) 634, 1287
Mva1269I GAATGC 1 cut(s) 1003
MvaI CCWGG 1 cut(s) 1287
MwoI GCNNNNNNNGC 3 cut(s) 13, 1016, 1223
NciI CCSGG 1 cut(s) 634
NdeII GATC 8 cut(s) 316, 401, 1060, 1103, 1239, 1438, 1453, 1497
NlaIII CATG 7 cut(s) 434, 532, 608, 1240, 1253, 1264, 1282
NlaIV GGNNCC 4 cut(s) 1217, 1291, 1353, 1455
NmuCI GTSAC 2 cut(s) 754, 844
NspV TTCGAA 2 cut(s) 925, 1035
OliI CACNNNNGTG 1 cut(s) 591
PaeR7I CTCGAG 2 cut(s) 734, 1401
PagI TCATGA 2 cut(s) 1236, 1278
PceI AGGCCT 1 cut(s) 1285
PctI GAATGC 1 cut(s) 1003
PfeI GAWTC 3 cut(s) 862, 884, 1393
PinAI ACCGGT 1 cut(s) 588
PkrI GCNGC 6 cut(s) 18, 61, 64, 188, 1258, 1333
Ple19I CGATCG 1 cut(s) 319
PleI GAGTC 1 cut(s) 74
PpsI GAGTC 1 cut(s) 74
Psp124BI GAGCTC 2 cut(s) 876, 1412
Psp6I CCWGG 1 cut(s) 1285
PspEI GGTNACC 1 cut(s) 844
PspFI CCCAGC 1 cut(s) 699
PspGI CCWGG 1 cut(s) 1285
PspN4I GGNNCC 4 cut(s) 1217, 1291, 1353, 1455
PspOMI GGGCCC 1 cut(s) 1215
PspPI GGNCC 3 cut(s) 1215, 1216, 1290
PstI CTGCAG 1 cut(s) 67
PsuI RGATCY 2 cut(s) 1453, 1497
PvuI CGATCG 1 cut(s) 319
PvuII CAGCTG 1 cut(s) 62
RsaI GTAC 3 cut(s) 740, 1418, 1487
RsaNI GTAC 3 cut(s) 739, 1417, 1486
RseI CAYNNNNRTG 2 cut(s) 511, 591
SacI GAGCTC 2 cut(s) 876, 1412
SaqAI TTAA 2 cut(s) 1167, 1221
SatI GCNGC 6 cut(s) 17, 60, 63, 187, 1257, 1332
Sau3AI GATC 8 cut(s) 316, 401, 1060, 1103, 1239, 1438, 1453, 1497
Sau96I GGNCC 3 cut(s) 1215, 1216, 1290
SchI GAGTC 1 cut(s) 74
ScrFI CCNGG 2 cut(s) 634, 1287
SduI GDGCHC 4 cut(s) 486, 876, 1219, 1412
SfaNI GCATC 3 cut(s) 28, 570, 1028
SfcI CTRYAG 1 cut(s) 63
Sfr274I CTCGAG 2 cut(s) 734, 1401
SfuI TTCGAA 2 cut(s) 925, 1035
SgrAI CRCCGGYG 1 cut(s) 588
SinI GGWCC 1 cut(s) 1290
SlaI CTCGAG 2 cut(s) 734, 1401
SmiMI CAYNNNNRTG 2 cut(s) 511, 591
SmlI CTYRAG 2 cut(s) 734, 1401
SmoI CTYRAG 2 cut(s) 734, 1401
Sse9I AATT 8 cut(s) 545, 662, 745, 957, 969, 1064, 1126, 1152
SseBI AGGCCT 1 cut(s) 1285
SsiI CCGC 5 cut(s) 7, 16, 1145, 1186, 1334
SspMI CTAG 2 cut(s) 935, 1316
SstI GAGCTC 2 cut(s) 876, 1412
StuI AGGCCT 1 cut(s) 1285
StyD4I CCNGG 2 cut(s) 632, 1285
StyI CCWWGG 1 cut(s) 1315
TaaI ACNGT 7 cut(s) 374, 501, 850, 880, 1430, 1519, 1614
TaqI TCGA 7 cut(s) 319, 735, 925, 955, 1035, 1096, 1402
TaqII GACCGA 1 cut(s) 954
TasI AATT 8 cut(s) 545, 662, 745, 957, 969, 1064, 1126, 1152
TatI WGTACW 1 cut(s) 1485
TauI GCSGC 1 cut(s) 19
TfiI GAWTC 3 cut(s) 862, 884, 1393
Tru1I TTAA 2 cut(s) 1167, 1221
Tru9I TTAA 2 cut(s) 1167, 1221
TscAI CASTG 5 cut(s) 38, 377, 624, 883, 897
TseFI GTSAC 2 cut(s) 754, 844
TseI GCWGC 5 cut(s) 59, 62, 186, 1256, 1331
Tsp45I GTSAC 2 cut(s) 754, 844
TspGWI ACGGA 2 cut(s) 523, 1282
TspRI CASTG 5 cut(s) 38, 377, 624, 883, 897
VpaK11BI GGWCC 1 cut(s) 1290
XapI RAATTY 2 cut(s) 662, 1126
XbaI TCTAGA 1 cut(s) 934
XhoI CTCGAG 2 cut(s) 734, 1401
XmaJI CCTAGG 1 cut(s) 1315
XmiI GTMKAC 2 cut(s) 503, 641
XspI CTAG 2 cut(s) 935, 1316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.