pycom12g06530

Protein of unknown function (DUF563)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002314
Physical Location & Seq
Forward (+)
6755339 .. 6758374
3036 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g06530.1

Sequence Viewer

Length: 1590 bp
ATGTATAGAAAATTAGTCACGTGGGGGCGGTATCAGCGGCATCACCACCACCAGTGGAGAAAAGTTGACAAGCCTTGTGCAGCAGCAGAGGGAGATGAAGACTCACAGACCACTACCTTTCTAATGGAGTTTGCAAACTCTGGTTATTATTACAAGAGAACAAGTCCAAAGCTTCTCTATCTCCTCTTCATCTCCTTCCTCTCTTGCAGCTTCATCTTAGCCCCTCATCTTTTCAGCTCCAACACCACTTTCTCTCTTCTATATTCAACACCAATGGAAGATATGAATGTAAATGGTCCCTTGTGTTCTTCAATCTCTAATGGAACTATATGTTGCGATCGAAGCAGTATTCGTTCAGATATTTGTGTAATGAAAGGGGATGTAAGAACACACTCTGCTTCCTCCACTATCTTTCTCTACCGATCTAAAGAAGATGAAACCGAGGAACTCAGGCACACAAAGGTCAAACCGTATACGCGGAAATGGGAAACAAGTGTCATGGATACCATTGATGAATTACAGCTCGTTGCAAAGAAAGACACTCCAGGGATGCACCACCGGTGTGATGTCCAACATGATGTTCCGGCATTGTTTTTCTCAACCGGGGGATATACCGGTAATGTCTATCACGAATTCAATGATGGGATTTTGCCGTTGTTCATTACTTCCCAGCGCTTCAACAAGAAGGTTGTGTTTGTCATTCTCGAGTACCATAATTGGTGGGTGACGAAATATGGAGACATTGTTTCTCAACTGTCAGATTATCAACCTATAGATTTTAGTGGAGATACAAAAACTCATTGCTTCCCTGAGGTCACCGTTGGTCTGAGGATTCACGACGAGCTCACTGTGGATTCTTCACTGATGGAGGGAAATGTGAGCATTGTTGACTTTCGAAATCTTCTAGACCGAGCTTACTGGCCTCGAATTAGAAGTCTTATTCAAGACGAGGAACGGGAAGCACAGGAGAAGCATTCTGCGTCTCTGGCATCCGAGAGCTCTTTCGAACCTGAGAGTGAAGTGCAAGAAGATCAATTCAAGAAGCCTAAACTGGTCATCATATCTCGAAATGGATCAAGAGCGATAACTAATGAGAATTTCTTGGTTAGAATGGCTGAGCAAATTGGGTTTGAAGTTAATGTTTTGAGACCTGACCGCACGACAGAGTTAGCAAAGATTTATCGGGCCCTTAATGCGAGTGATGTGATGATCGGGGTTCATGGCGCCGCCATGACACATTTTATGTTCATGAGGCCTGGGTCTGTGTTCATCCAAGTTGTTCCCCTAGGAACTGAGTGGGCAGCGGAGGAATACTATGGGGAACCTGCAAGGAAGCTCGGGTTGAAATACATCGGCTACCAGATTCTTACTCGAGAGAGCTCGTTGTACGCCAAATACGATAAGGATGATCGATCTGTTCTTAGGGATCCACAGAGTGTAACCAAAATGGGATGGGAGTACACAAAGAAGATCTATCTTGATGGCCAAACTGTGAGATTAGACCTCCGAAGATTTCGGAAGCGATTGGTTCGTGCTTACGATTACATCTTCCACAGGTTGAACAATCACCCCCATCTTCGATCACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
DUF563

Protein Analysis

530

Amino Acids

61.4

Weight (kDa)

7.31

Isoelectric Point (pI)

35.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 313 - 424 2.3e-14 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1333
AccB1I GGYRCC 1 cut(s) 1223
AccI GTMKAC 1 cut(s) 473
AccII CGCG 1 cut(s) 478
AciI CCGC 6 cut(s) 28, 37, 478, 1156, 1227, 1304
AclWI GGATC 3 cut(s) 1081, 1421, 1434
AcoI YGGCCR 1 cut(s) 1483
AcsI RAATTY 2 cut(s) 632, 1096
AcvI CACGTG 1 cut(s) 21
AcyI GRCGYC 1 cut(s) 1224
AdeI CACNNNGTG 1 cut(s) 1436
AfaI GTAC 3 cut(s) 710, 1388, 1460
AfeI AGCGCT 1 cut(s) 674
AgeI ACCGGT 2 cut(s) 558, 614
AgsI TTSAA 9 cut(s) 267, 312, 637, 679, 944, 1039, 1133, 1345, 1561
AjnI CCWGG 2 cut(s) 544, 1255
AjuI GAANNNNNNNTTGG 4 cut(s) 233, 265, 564, 596
AleI CACNNNNGTG 1 cut(s) 561
AluBI AGCT 9 cut(s) 172, 210, 237, 523, 844, 914, 999, 1336, 1380
AluI AGCT 9 cut(s) 172, 210, 237, 523, 844, 914, 999, 1336, 1380
Alw21I GWGCWC 3 cut(s) 846, 1001, 1382
Alw26I GTCTC 3 cut(s) 732, 987, 1141
AlwI GGATC 3 cut(s) 1081, 1421, 1434
Ama87I CYCGRG 3 cut(s) 704, 1337, 1371
Aor51HI AGCGCT 1 cut(s) 674
AoxI GGCC 4 cut(s) 920, 1185, 1253, 1483
ApaI GGGCCC 1 cut(s) 1189
ApeKI GCWGC 4 cut(s) 80, 83, 207, 1301
ApoI RAATTY 2 cut(s) 632, 1096
ArsI GACNNNNNNTTYG 2 cut(s) 919, 951
AsiGI ACCGGT 2 cut(s) 558, 614
AspA2I CCTAGG 1 cut(s) 1285
AspLEI GCGC 2 cut(s) 675, 1226
AspS9I GGNCC 3 cut(s) 296, 1185, 1186
AsuC2I CCSGG 1 cut(s) 604
AsuHPI GGTGA 4 cut(s) 35, 736, 808, 1559
AsuII TTCGAA 2 cut(s) 895, 1005
AvaI CYCGRG 3 cut(s) 704, 1337, 1371
AvaII GGWCC 1 cut(s) 296
AvrII CCTAGG 1 cut(s) 1285
AxyI CCTNAGG 1 cut(s) 810
BaeGI GKGCMC 1 cut(s) 1189
BalI TGGCCA 1 cut(s) 1485
BamHI GGATCC 1 cut(s) 1426
BanI GGYRCC 1 cut(s) 1223
BanII GRGCYC 4 cut(s) 846, 1001, 1189, 1382
BbrPI CACGTG 1 cut(s) 21
BbsI GAAGAC 1 cut(s) 105
Bbv12I GWGCWC 3 cut(s) 846, 1001, 1382
BbvI GCAGC 4 cut(s) 92, 95, 219, 1313
BccI CCATC 5 cut(s) 635, 859, 1446, 1475, 1581
BceAI ACGGC 1 cut(s) 637
BciT130I CCWGG 2 cut(s) 546, 1257
BciVI GTATCC 1 cut(s) 496
BcnI CCSGG 1 cut(s) 604
BcoDI GTCTC 3 cut(s) 732, 987, 1141
BfaI CTAG 2 cut(s) 905, 1286
BfmI CTRYAG 1 cut(s) 771
BfoI RGCGCY 2 cut(s) 676, 1227
BfuAI ACCTGC 1 cut(s) 1333
BfuI GTATCC 1 cut(s) 496
BglII AGATCT 1 cut(s) 1470
BisI GCNGC 6 cut(s) 38, 81, 84, 208, 1227, 1302
BlnI CCTAGG 1 cut(s) 1285
BlpI GCTNAGC 1 cut(s) 1116
BlsI GCNGC 6 cut(s) 39, 82, 85, 209, 1228, 1303
Bme1390I CCNGG 3 cut(s) 546, 604, 1257
Bme18I GGWCC 1 cut(s) 296
BmeT110I CYCGRG 3 cut(s) 704, 1337, 1371
BmgT120I GGNCC 3 cut(s) 296, 1185, 1186
BmiI GGNNCC 5 cut(s) 298, 1187, 1225, 1323, 1428
BmrFI CCNGG 3 cut(s) 546, 604, 1257
BmsI GCATC 3 cut(s) 49, 540, 998
BpiI GAAGAC 1 cut(s) 105
BpmI CTGGAG 1 cut(s) 528
Bpu1102I GCTNAGC 1 cut(s) 1116
Bpu14I TTCGAA 2 cut(s) 895, 1005
BpuMI CCSGG 1 cut(s) 604
Bsa29I ATCGAT 1 cut(s) 1411
BsaAI YACGTR 1 cut(s) 21
BsaHI GRCGYC 1 cut(s) 1224
BsaI GGTCTC 1 cut(s) 1141
BsaJI CCNNGG 5 cut(s) 441, 545, 603, 1256, 1285
BsaWI WCCGGW 2 cut(s) 558, 614
BsaXI ACNNNNNCTCC 2 cut(s) 729, 759
Bse118I RCCGGY 2 cut(s) 558, 614
Bse1I ACTGG 3 cut(s) 52, 923, 1056
Bse21I CCTNAGG 1 cut(s) 810
Bse3DI GCAATG 1 cut(s) 799
BseBI CCWGG 2 cut(s) 546, 1257
BseCI ATCGAT 1 cut(s) 1411
BseDI CCNNGG 5 cut(s) 441, 545, 603, 1256, 1285
BseGI GGATG 6 cut(s) 385, 555, 989, 1269, 1411, 1457
BseMI GCAATG 1 cut(s) 799
BseMII CTCAG 6 cut(s) 463, 801, 818, 1002, 1107, 1284
BseNI ACTGG 3 cut(s) 52, 923, 1056
BseRI GAGGAG 1 cut(s) 173
BseSI GKGCMC 1 cut(s) 1189
BseXI GCAGC 4 cut(s) 92, 95, 219, 1313
BseYI CCCAGC 1 cut(s) 669
BsgI GTGCAG 1 cut(s) 99
Bsh1236I CGCG 1 cut(s) 478
Bsh1285I CGRYCG 1 cut(s) 340
BshFI GGCC 4 cut(s) 922, 1187, 1255, 1485
BshNI GGYRCC 1 cut(s) 1223
BshTI ACCGGT 2 cut(s) 558, 614
BshVI ATCGAT 1 cut(s) 1411
BsiEI CGRYCG 1 cut(s) 340
BsiHKAI GWGCWC 3 cut(s) 846, 1001, 1382
BsiHKCI CYCGRG 3 cut(s) 704, 1337, 1371
BsiSI CCGG 4 cut(s) 559, 584, 603, 615
BslFI GGGAC 1 cut(s) 282
BsmAI GTCTC 3 cut(s) 732, 987, 1141
BsmBI CGTCTC 1 cut(s) 987
BsmFI GGGAC 1 cut(s) 282
BsmI GAATGC 1 cut(s) 973
BsnI GGCC 4 cut(s) 922, 1187, 1255, 1485
Bso31I GGTCTC 1 cut(s) 1141
BsoBI CYCGRG 3 cut(s) 704, 1337, 1371
Bsp119I TTCGAA 2 cut(s) 895, 1005
Bsp120I GGGCCC 1 cut(s) 1185
Bsp1286I GDGCHC 4 cut(s) 846, 1001, 1189, 1382
Bsp1720I GCTNAGC 1 cut(s) 1116
BspACI CCGC 6 cut(s) 28, 37, 478, 1156, 1227, 1304
BspANI GGCC 4 cut(s) 922, 1187, 1255, 1485
BspCNI CTCAG 6 cut(s) 462, 802, 819, 1003, 1108, 1285
BspDI ATCGAT 1 cut(s) 1411
BspFNI CGCG 1 cut(s) 478
BspHI TCATGA 1 cut(s) 1248
BspLI GGNNCC 5 cut(s) 298, 1187, 1225, 1323, 1428
BspMI ACCTGC 1 cut(s) 1333
BspPI GGATC 3 cut(s) 1081, 1421, 1434
BspT104I TTCGAA 2 cut(s) 895, 1005
BspT107I GGYRCC 1 cut(s) 1223
BspTNI GGTCTC 1 cut(s) 1141
BsrDI GCAATG 1 cut(s) 799
BsrFI RCCGGY 2 cut(s) 558, 614
BsrI ACTGG 3 cut(s) 52, 923, 1056
BssAI RCCGGY 2 cut(s) 558, 614
BssECI CCNNGG 5 cut(s) 441, 545, 603, 1256, 1285
BssNAI GTATAC 1 cut(s) 474
BssNI GRCGYC 1 cut(s) 1224
BssT1I CCWWGG 1 cut(s) 1285
Bst1107I GTATAC 1 cut(s) 474
Bst2UI CCWGG 2 cut(s) 546, 1257
Bst4CI ACNGT 6 cut(s) 471, 756, 820, 850, 1492, 1587
Bst6I CTCTTC 2 cut(s) 191, 261
BstACI GRCGYC 1 cut(s) 1224
BstBAI YACGTR 1 cut(s) 21
BstBI TTCGAA 2 cut(s) 895, 1005
BstDEI CTNAG 8 cut(s) 217, 449, 810, 827, 1011, 1116, 1293, 1421
BstEII GGTNACC 1 cut(s) 814
BstF5I GGATG 6 cut(s) 385, 555, 989, 1269, 1411, 1457
BstFNI CGCG 1 cut(s) 478
BstH2I RGCGCY 2 cut(s) 676, 1227
BstHHI GCGC 2 cut(s) 675, 1226
BstMAI GTCTC 3 cut(s) 732, 987, 1141
BstMCI CGRYCG 1 cut(s) 340
BstMWI GCNNNNNNNGC 4 cut(s) 34, 342, 986, 1193
BstNI CCWGG 2 cut(s) 546, 1257
BstPI GGTNACC 1 cut(s) 814
BstSCI CCNGG 3 cut(s) 544, 602, 1255
BstSFI CTRYAG 1 cut(s) 771
BstSLI GKGCMC 1 cut(s) 1189
BstUI CGCG 1 cut(s) 478
BstV1I GCAGC 4 cut(s) 92, 95, 219, 1313
BstV2I GAAGAC 1 cut(s) 105
BstX2I RGATCY 2 cut(s) 1426, 1470
BstYI RGATCY 2 cut(s) 1426, 1470
BstZ17I GTATAC 1 cut(s) 474
Bsu15I ATCGAT 1 cut(s) 1411
Bsu36I CCTNAGG 1 cut(s) 810
BsuI GTATCC 1 cut(s) 496
BsuRI GGCC 4 cut(s) 922, 1187, 1255, 1485
BsuTUI ATCGAT 1 cut(s) 1411
BtsCI GGATG 6 cut(s) 385, 555, 989, 1269, 1411, 1457
BtsIMutI CAGTG 3 cut(s) 59, 846, 860
BveI ACCTGC 1 cut(s) 1333
CciI TCATGA 1 cut(s) 1248
CfoI GCGC 2 cut(s) 675, 1226
Cfr10I RCCGGY 2 cut(s) 558, 614
Cfr13I GGNCC 3 cut(s) 296, 1185, 1186
ClaI ATCGAT 1 cut(s) 1411
CseI GACGC 1 cut(s) 969
Csp6I GTAC 3 cut(s) 709, 1387, 1459
CspAI ACCGGT 2 cut(s) 558, 614
CviAII CATG 5 cut(s) 499, 575, 1220, 1231, 1249
CviQI GTAC 3 cut(s) 709, 1387, 1459
DdeI CTNAG 8 cut(s) 217, 449, 810, 827, 1011, 1116, 1293, 1421
DinI GGCGCC 1 cut(s) 1225
DraIII CACNNNGTG 1 cut(s) 1436
EaeI YGGCCR 1 cut(s) 1483
Eam1104I CTCTTC 2 cut(s) 191, 261
EarI CTCTTC 2 cut(s) 191, 261
Ecl136II GAGCTC 3 cut(s) 844, 999, 1380
Eco130I CCWWGG 1 cut(s) 1285
Eco147I AGGCCT 1 cut(s) 1255
Eco24I GRGCYC 4 cut(s) 846, 1001, 1189, 1382
Eco31I GGTCTC 1 cut(s) 1141
Eco47I GGWCC 1 cut(s) 296
Eco47III AGCGCT 1 cut(s) 674
Eco53kI GAGCTC 3 cut(s) 844, 999, 1380
Eco72I CACGTG 1 cut(s) 21
Eco81I CCTNAGG 1 cut(s) 810
Eco88I CYCGRG 3 cut(s) 704, 1337, 1371
Eco91I GGTNACC 1 cut(s) 814
EcoICRI GAGCTC 3 cut(s) 844, 999, 1380
EcoO109I RGGNCCY 1 cut(s) 1186
EcoO65I GGTNACC 1 cut(s) 814
EcoRI GAATTC 1 cut(s) 632
EcoRII CCWGG 2 cut(s) 544, 1255
EcoT14I CCWWGG 1 cut(s) 1285
EcoT38I GRGCYC 4 cut(s) 846, 1001, 1189, 1382
EgeI GGCGCC 1 cut(s) 1225
EheI GGCGCC 1 cut(s) 1225
ErhI CCWWGG 1 cut(s) 1285
Esp3I CGTCTC 1 cut(s) 987
FaeI CATG 5 cut(s) 502, 578, 1223, 1234, 1252
FaqI GGGAC 1 cut(s) 282
FatI CATG 5 cut(s) 498, 574, 1219, 1230, 1248
FblI GTMKAC 1 cut(s) 473
Fnu4HI GCNGC 6 cut(s) 38, 81, 84, 208, 1227, 1302
FokI GGATG 6 cut(s) 392, 562, 976, 1256, 1418, 1464
FriOI GRGCYC 4 cut(s) 846, 1001, 1189, 1382
Fsp4HI GCNGC 6 cut(s) 38, 81, 84, 208, 1227, 1302
FspBI CTAG 2 cut(s) 905, 1286
GlaI GCGC 2 cut(s) 674, 1225
GluI GCNGC 6 cut(s) 38, 81, 84, 208, 1227, 1302
GsaI CCCAGC 1 cut(s) 673
GsuI CTGGAG 1 cut(s) 528
HaeII RGCGCY 2 cut(s) 676, 1227
HaeIII GGCC 4 cut(s) 922, 1187, 1255, 1485
HapII CCGG 4 cut(s) 559, 584, 603, 615
HgaI GACGC 1 cut(s) 969
HhaI GCGC 2 cut(s) 675, 1226
Hin1I GRCGYC 1 cut(s) 1224
Hin1II CATG 5 cut(s) 502, 578, 1223, 1234, 1252
Hin6I GCGC 2 cut(s) 673, 1224
HinP1I GCGC 2 cut(s) 673, 1224
HincII GTYRAC 2 cut(s) 67, 889
HindII GTYRAC 2 cut(s) 67, 889
HindIII AAGCTT 1 cut(s) 170
HinfI GANTC 4 cut(s) 101, 832, 854, 1363
HpaII CCGG 4 cut(s) 559, 584, 603, 615
HphI GGTGA 4 cut(s) 35, 736, 808, 1559
Hpy166II GTNNAC 4 cut(s) 67, 474, 889, 1461
Hpy188I TCNGA 6 cut(s) 358, 760, 828, 994, 1508, 1518
Hpy8I GTNNAC 4 cut(s) 67, 474, 889, 1461
Hpy99I CGWCG 1 cut(s) 842
HpyAV CCTTC 2 cut(s) 205, 679
HpyCH4III ACNGT 6 cut(s) 471, 756, 820, 850, 1492, 1587
HpyCH4IV ACGT 1 cut(s) 20
HpyCH4V TGCA 7 cut(s) 80, 134, 207, 530, 553, 1024, 1328
HpyF10VI GCNNNNNNNGC 4 cut(s) 34, 342, 986, 1193
HpyF3I CTNAG 8 cut(s) 217, 449, 810, 827, 1011, 1116, 1293, 1421
HpySE526I ACGT 1 cut(s) 20
Hsp92I GRCGYC 1 cut(s) 1224
Hsp92II CATG 5 cut(s) 502, 578, 1223, 1234, 1252
HspAI GCGC 2 cut(s) 673, 1224
KasI GGCGCC 1 cut(s) 1223
LmnI GCTCC 1 cut(s) 242
Lsp1109I GCAGC 4 cut(s) 92, 95, 219, 1313
LweI GCATC 3 cut(s) 49, 540, 998
MaeI CTAG 2 cut(s) 905, 1286
MaeII ACGT 1 cut(s) 20
MaeIII GTNAC 4 cut(s) 16, 724, 814, 1438
MflI RGATCY 2 cut(s) 1426, 1470
MhlI GDGCHC 4 cut(s) 846, 1001, 1189, 1382
MlsI TGGCCA 1 cut(s) 1485
MluCI AATT 8 cut(s) 11, 515, 632, 715, 927, 1034, 1096, 1122
MluNI TGGCCA 1 cut(s) 1485
Mly113I GGCGCC 1 cut(s) 1224
MlyI GAGTC 1 cut(s) 95
MmeI TCCRAC 2 cut(s) 264, 595
Mox20I TGGCCA 1 cut(s) 1485
MscI TGGCCA 1 cut(s) 1485
MseI TTAA 2 cut(s) 1137, 1191
MslI CAYNNNNRTG 1 cut(s) 561
Msp20I TGGCCA 1 cut(s) 1485
MspA1I CMGCKG 2 cut(s) 37, 1304
MspI CCGG 4 cut(s) 559, 584, 603, 615
MspR9I CCNGG 3 cut(s) 546, 604, 1257
Mva1269I GAATGC 1 cut(s) 973
MvaI CCWGG 2 cut(s) 546, 1257
MvnI CGCG 1 cut(s) 478
MwoI GCNNNNNNNGC 4 cut(s) 34, 342, 986, 1193
NarI GGCGCC 1 cut(s) 1224
NciI CCSGG 1 cut(s) 604
NlaIII CATG 5 cut(s) 502, 578, 1223, 1234, 1252
NlaIV GGNNCC 5 cut(s) 298, 1187, 1225, 1323, 1428
NmuCI GTSAC 3 cut(s) 16, 724, 814
NspV TTCGAA 2 cut(s) 895, 1005
OliI CACNNNNGTG 1 cut(s) 561
PaeR7I CTCGAG 2 cut(s) 704, 1371
PagI TCATGA 1 cut(s) 1248
PceI AGGCCT 1 cut(s) 1255
PcsI WCGNNNNNNNCGW 1 cut(s) 1395
PctI GAATGC 1 cut(s) 973
PfeI GAWTC 3 cut(s) 832, 854, 1363
PinAI ACCGGT 2 cut(s) 558, 614
PkrI GCNGC 6 cut(s) 39, 82, 85, 209, 1228, 1303
Ple19I CGATCG 1 cut(s) 340
PleI GAGTC 1 cut(s) 95
PluTI GGCGCC 1 cut(s) 1227
PmaCI CACGTG 1 cut(s) 21
PmlI CACGTG 1 cut(s) 21
PpsI GAGTC 1 cut(s) 95
Ppu21I YACGTR 1 cut(s) 21
Psp124BI GAGCTC 3 cut(s) 846, 1001, 1382
Psp6I CCWGG 2 cut(s) 544, 1255
PspCI CACGTG 1 cut(s) 21
PspEI GGTNACC 1 cut(s) 814
PspFI CCCAGC 1 cut(s) 669
PspGI CCWGG 2 cut(s) 544, 1255
PspN4I GGNNCC 5 cut(s) 298, 1187, 1225, 1323, 1428
PspOMI GGGCCC 1 cut(s) 1185
PspPI GGNCC 3 cut(s) 296, 1185, 1186
PsuI RGATCY 2 cut(s) 1426, 1470
PvuI CGATCG 1 cut(s) 340
RsaI GTAC 3 cut(s) 710, 1388, 1460
RsaNI GTAC 3 cut(s) 709, 1387, 1459
RseI CAYNNNNRTG 1 cut(s) 561
SacI GAGCTC 3 cut(s) 846, 1001, 1382
SaqAI TTAA 2 cut(s) 1137, 1191
SatI GCNGC 6 cut(s) 38, 81, 84, 208, 1227, 1302
Sau96I GGNCC 3 cut(s) 296, 1185, 1186
SchI GAGTC 1 cut(s) 95
ScrFI CCNGG 3 cut(s) 546, 604, 1257
SduI GDGCHC 4 cut(s) 846, 1001, 1189, 1382
SfaNI GCATC 3 cut(s) 49, 540, 998
SfcI CTRYAG 1 cut(s) 771
SfoI GGCGCC 1 cut(s) 1225
Sfr274I CTCGAG 2 cut(s) 704, 1371
SfuI TTCGAA 2 cut(s) 895, 1005
SgrAI CRCCGGYG 1 cut(s) 558
SinI GGWCC 1 cut(s) 296
SlaI CTCGAG 2 cut(s) 704, 1371
SmiMI CAYNNNNRTG 1 cut(s) 561
SmlI CTYRAG 2 cut(s) 704, 1371
SmoI CTYRAG 2 cut(s) 704, 1371
Sse9I AATT 8 cut(s) 11, 515, 632, 715, 927, 1034, 1096, 1122
SseBI AGGCCT 1 cut(s) 1255
SsiI CCGC 6 cut(s) 28, 37, 478, 1156, 1227, 1304
SspDI GGCGCC 1 cut(s) 1223
SspMI CTAG 2 cut(s) 905, 1286
SstI GAGCTC 3 cut(s) 846, 1001, 1382
StuI AGGCCT 1 cut(s) 1255
StyD4I CCNGG 3 cut(s) 544, 602, 1255
StyI CCWWGG 1 cut(s) 1285
TaaI ACNGT 6 cut(s) 471, 756, 820, 850, 1492, 1587
TaiI ACGT 1 cut(s) 23
TaqI TCGA 9 cut(s) 340, 705, 895, 925, 1005, 1066, 1372, 1411, 1579
TaqII GACCGA 1 cut(s) 924
TasI AATT 8 cut(s) 11, 515, 632, 715, 927, 1034, 1096, 1122
TatI WGTACW 1 cut(s) 1458
TauI GCSGC 2 cut(s) 40, 1229
TfiI GAWTC 3 cut(s) 832, 854, 1363
Tru1I TTAA 2 cut(s) 1137, 1191
Tru9I TTAA 2 cut(s) 1137, 1191
TscAI CASTG 3 cut(s) 59, 853, 867
TseFI GTSAC 3 cut(s) 16, 724, 814
TseI GCWGC 4 cut(s) 80, 83, 207, 1301
Tsp45I GTSAC 3 cut(s) 16, 724, 814
TspRI CASTG 3 cut(s) 59, 853, 867
VpaK11BI GGWCC 1 cut(s) 296
XapI RAATTY 2 cut(s) 632, 1096
XbaI TCTAGA 1 cut(s) 904
XhoI CTCGAG 2 cut(s) 704, 1371
XmaJI CCTAGG 1 cut(s) 1285
XmiI GTMKAC 1 cut(s) 473
XspI CTAG 2 cut(s) 905, 1286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.