Rh3AG253100

Protein of unknown function (DUF563)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0002314
Physical Location & Seq
Reverse (-)
27433997 .. 27437263
3267 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG253100.1

Sequence Viewer

Length: 1266 bp
ATGAAAGGGGATGTGAGAACCCAGTCTACTTCCTTTTCGATCTTTGTCTATGGATCTGAAGACAGAAGTAGCTCCAAAAGTCTGTTTTCAGGTATTGTTGAGGAAAATGAGGAACTCCAACACGAAAGGATCAAACCATACACCCGGAAATGGGAAACTAGTGTGATGAACACCATTGATGAATTACACCTCATTGCAAAGAAAGACAGTACTCTAGGCCCGTACCAACAACAGTGTGATGTCCAACATGATGTTCCAGCTGTGTTTTTCTCGACTGGGGGCTATACCGGTAATGTTTATCATGAATTCAATGATGGGATTGTGCCATTGTACATTACCTCCCAGCATTTCGACAAAAAGGTTGTGTTTGTCATTCAGGATTATCATAATTGGTGGGTCATGAAATATGGAGACATTCTTTCACAGCTATCAGATTATCCACCTATAGACTTCAGTGGAGATAAGAGAACTCATTGCTTCCCTGAGGCCATTGTTGGTCTGAGAATTCATGACGAGCTTTCTGTTGATTCTTCACTGATGGAGAGGAATAAGGACATTGTTGATTTCCGGGATGTCCTAGACAGAGCTTACTGGCCTCGAATTAGAGGTCTAATTCAAGATGAGGAACGAGAAGCGCAGGAGAAGCTTTCAGTGTCTCCCACATTTGAAAGCTCCTTAGAAATCAAAAAAGAAGTGCAAGAAGATCAACTTTTGAAGAAGCCTAAACTGCTAATCATATCTCGGAATGGATCAAGAGCGATAACTAATGAAAATTTATTGGTGAAGATGGCTGAGAAAATCGGTTTTGAAGTTGAGGTTTTGAGACCAGACCGCACGACAGAGTTGGCAAAGATTTATCGGGCACTTAATGCGAGTGATGTCATGATTGGAGTTCATGGTGCTGCTATGACTCATTTTCTCTTCATGAAGCCGCACTCTGTTTTCATCCAAGTTATTCCTCTCGGAACTGAATGGGCAGCAGAAGAGTATTATGGAGAACCTGCAAAGAAGCTTGGCTTGAAATACATTGGCTATCAAATTCTCGCAAGAGAGAGCTCTTTGTATGGCAAATATGATAGAGATGATCCTGTTCTTCGAGATCCACAGAGCGTAAACAAAAAGGGATGGGAATACACAAAGAAAATCTATCTTGATGGCCAAACTGTGAGATTAGACCTTGGGAGATTTCGGAAACGTTTGGTTCGTGCTTATGATTACATGTTGAGTAGAATGAACCAGCGTCCCCATCTGCAATATTCACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
DUF563

Protein Analysis

421

Amino Acids

48.89

Weight (kDa)

6.34

Isoelectric Point (pI)

33.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 230 - 316 2.5e-14 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1009
AccI GTMKAC 1 cut(s) 26
AciI CCGC 2 cut(s) 832, 932
AclI AACGTT 1 cut(s) 1195
AclWI GGATC 5 cut(s) 61, 137, 757, 1079, 1094
AcoI YGGCCR 1 cut(s) 1156
AcsI RAATTY 4 cut(s) 305, 504, 772, 1038
AcuI CTGAAG 2 cut(s) 78, 436
AfaI GTAC 3 cut(s) 211, 224, 332
AfiI CCNNNNNNNGG 2 cut(s) 150, 151
AflIII ACRYGT 1 cut(s) 1218
AgeI ACCGGT 1 cut(s) 287
AgsI TTSAA 6 cut(s) 310, 617, 668, 715, 809, 1021
AhlI ACTAGT 1 cut(s) 158
AjuI GAANNNNNNNTTGG 2 cut(s) 237, 269
AluBI AGCT 9 cut(s) 72, 260, 427, 517, 587, 646, 672, 1012, 1056
AluI AGCT 9 cut(s) 72, 260, 427, 517, 587, 646, 672, 1012, 1056
Alw21I GWGCWC 1 cut(s) 1058
Alw26I GTCTC 3 cut(s) 405, 660, 817
AlwI GGATC 5 cut(s) 61, 137, 757, 1079, 1094
AoxI GGCC 4 cut(s) 217, 486, 593, 1156
ApeKI GCWGC 2 cut(s) 902, 977
ApoI RAATTY 4 cut(s) 305, 504, 772, 1038
ArsI GACNNNNNNTTYG 2 cut(s) 592, 624
AsiGI ACCGGT 1 cut(s) 287
AspLEI GCGC 1 cut(s) 637
AspS9I GGNCC 1 cut(s) 218
AsuC2I CCSGG 2 cut(s) 145, 569
AsuHPI GGTGA 1 cut(s) 793
AxyI CCTNAGG 1 cut(s) 483
BaeGI GKGCMC 1 cut(s) 865
BalI TGGCCA 1 cut(s) 1158
BanII GRGCYC 1 cut(s) 1058
BbsI GAAGAC 1 cut(s) 66
Bbv12I GWGCWC 1 cut(s) 1058
BbvI GCAGC 2 cut(s) 889, 989
BccI CCATC 6 cut(s) 308, 532, 781, 1119, 1148, 1254
BcnI CCSGG 2 cut(s) 145, 569
BcoDI GTCTC 3 cut(s) 405, 660, 817
BcuI ACTAGT 1 cut(s) 158
BfaI CTAG 3 cut(s) 159, 215, 578
BfmI CTRYAG 1 cut(s) 444
BfuAI ACCTGC 1 cut(s) 1009
BisI GCNGC 3 cut(s) 903, 932, 978
BlsI GCNGC 3 cut(s) 904, 933, 979
BmcAI AGTACT 1 cut(s) 211
Bme1390I CCNGG 2 cut(s) 145, 569
BmgT120I GGNCC 1 cut(s) 218
BmrFI CCNGG 2 cut(s) 145, 569
BmrI ACTGGG 2 cut(s) 16, 285
BmuI ACTGGG 2 cut(s) 16, 285
BpiI GAAGAC 1 cut(s) 66
BpuMI CCSGG 2 cut(s) 145, 569
BsaI GGTCTC 1 cut(s) 817
BsaJI CCNNGG 1 cut(s) 1177
BsaWI WCCGGW 1 cut(s) 287
BsaXI ACNNNNNCTCC 2 cut(s) 323, 353
Bsc4I CCNNNNNNNGG 2 cut(s) 150, 151
Bse118I RCCGGY 1 cut(s) 287
Bse1I ACTGG 3 cut(s) 22, 280, 596
Bse21I CCTNAGG 1 cut(s) 483
Bse3DI GCAATG 2 cut(s) 192, 472
BseDI CCNNGG 1 cut(s) 1177
BseGI GGATG 4 cut(s) 16, 577, 945, 1130
BseLI CCNNNNNNNGG 2 cut(s) 150, 151
BseMI GCAATG 2 cut(s) 192, 472
BseMII CTCAG 3 cut(s) 474, 491, 783
BseNI ACTGG 3 cut(s) 22, 280, 596
BseSI GKGCMC 1 cut(s) 865
BseXI GCAGC 2 cut(s) 889, 989
BseYI CCCAGC 1 cut(s) 342
BshFI GGCC 4 cut(s) 219, 488, 595, 1158
BshTI ACCGGT 1 cut(s) 287
BsiHKAI GWGCWC 1 cut(s) 1058
BsiSI CCGG 3 cut(s) 145, 288, 568
BslFI GGGAC 1 cut(s) 1227
BslI CCNNNNNNNGG 2 cut(s) 150, 151
BsmAI GTCTC 3 cut(s) 405, 660, 817
BsmFI GGGAC 1 cut(s) 1227
BsnI GGCC 4 cut(s) 219, 488, 595, 1158
Bso31I GGTCTC 1 cut(s) 817
Bsp1286I GDGCHC 2 cut(s) 865, 1058
Bsp1407I TGTACA 1 cut(s) 330
Bsp143I GATC 7 cut(s) 39, 53, 129, 703, 749, 1084, 1099
BspACI CCGC 2 cut(s) 832, 932
BspANI GGCC 4 cut(s) 219, 488, 595, 1158
BspCNI CTCAG 3 cut(s) 475, 492, 784
BspHI TCATGA 5 cut(s) 301, 399, 508, 882, 924
BspMI ACCTGC 1 cut(s) 1009
BspPI GGATC 5 cut(s) 61, 137, 757, 1079, 1094
BspTNI GGTCTC 1 cut(s) 817
BsrDI GCAATG 2 cut(s) 192, 472
BsrFI RCCGGY 1 cut(s) 287
BsrGI TGTACA 1 cut(s) 330
BsrI ACTGG 3 cut(s) 22, 280, 596
BssAI RCCGGY 1 cut(s) 287
BssECI CCNNGG 1 cut(s) 1177
BssMI GATC 7 cut(s) 39, 53, 129, 703, 749, 1084, 1099
BssT1I CCWWGG 1 cut(s) 1177
Bst4CI ACNGT 4 cut(s) 209, 234, 1165, 1263
Bst6I CTCTTC 2 cut(s) 926, 978
BstAPI GCANNNNNTGC 1 cut(s) 869
BstAUI TGTACA 1 cut(s) 330
BstDEI CTNAG 4 cut(s) 483, 500, 676, 792
BstF5I GGATG 4 cut(s) 16, 577, 945, 1130
BstHHI GCGC 1 cut(s) 637
BstKTI GATC 7 cut(s) 42, 56, 132, 706, 752, 1087, 1102
BstMAI GTCTC 3 cut(s) 405, 660, 817
BstMBI GATC 7 cut(s) 39, 53, 129, 703, 749, 1084, 1099
BstMWI GCNNNNNNNGC 3 cut(s) 643, 727, 869
BstNSI RCATGY 1 cut(s) 1222
BstSCI CCNGG 2 cut(s) 143, 567
BstSFI CTRYAG 1 cut(s) 444
BstSLI GKGCMC 1 cut(s) 865
BstV1I GCAGC 2 cut(s) 889, 989
BstV2I GAAGAC 1 cut(s) 66
BstX2I RGATCY 2 cut(s) 53, 1099
BstYI RGATCY 2 cut(s) 53, 1099
Bsu36I CCTNAGG 1 cut(s) 483
BsuRI GGCC 4 cut(s) 219, 488, 595, 1158
BtsCI GGATG 4 cut(s) 16, 577, 945, 1130
BtsIMutI CAGTG 4 cut(s) 239, 460, 533, 657
BveI ACCTGC 1 cut(s) 1009
CciI TCATGA 5 cut(s) 301, 399, 508, 882, 924
CfoI GCGC 1 cut(s) 637
Cfr10I RCCGGY 1 cut(s) 287
Cfr13I GGNCC 1 cut(s) 218
CseI GACGC 1 cut(s) 1229
Csp6I GTAC 3 cut(s) 210, 223, 331
CspAI ACCGGT 1 cut(s) 287
CviAII CATG 8 cut(s) 248, 302, 400, 509, 883, 896, 925, 1219
CviQI GTAC 3 cut(s) 210, 223, 331
DdeI CTNAG 4 cut(s) 483, 500, 676, 792
DpnI GATC 7 cut(s) 41, 55, 131, 705, 751, 1086, 1101
DpnII GATC 7 cut(s) 39, 53, 129, 703, 749, 1084, 1099
EaeI YGGCCR 1 cut(s) 1156
Eam1104I CTCTTC 2 cut(s) 926, 978
EarI CTCTTC 2 cut(s) 926, 978
Ecl136II GAGCTC 1 cut(s) 1056
Eco130I CCWWGG 1 cut(s) 1177
Eco24I GRGCYC 1 cut(s) 1058
Eco31I GGTCTC 1 cut(s) 817
Eco53kI GAGCTC 1 cut(s) 1056
Eco57I CTGAAG 2 cut(s) 78, 436
Eco81I CCTNAGG 1 cut(s) 483
EcoICRI GAGCTC 1 cut(s) 1056
EcoRI GAATTC 2 cut(s) 305, 504
EcoT14I CCWWGG 1 cut(s) 1177
EcoT38I GRGCYC 1 cut(s) 1058
ErhI CCWWGG 1 cut(s) 1177
FaeI CATG 8 cut(s) 251, 305, 403, 512, 886, 899, 928, 1222
FalI AAGNNNNNCTT 4 cut(s) 693, 725, 1001, 1033
FaqI GGGAC 1 cut(s) 1227
FatI CATG 8 cut(s) 247, 301, 399, 508, 882, 895, 924, 1218
FblI GTMKAC 1 cut(s) 26
Fnu4HI GCNGC 3 cut(s) 903, 932, 978
FokI GGATG 4 cut(s) 23, 584, 932, 1137
FriOI GRGCYC 1 cut(s) 1058
Fsp4HI GCNGC 3 cut(s) 903, 932, 978
FspBI CTAG 3 cut(s) 159, 215, 578
GlaI GCGC 1 cut(s) 636
GluI GCNGC 3 cut(s) 903, 932, 978
GsaI CCCAGC 1 cut(s) 346
HaeIII GGCC 4 cut(s) 219, 488, 595, 1158
HapII CCGG 3 cut(s) 145, 288, 568
HgaI GACGC 1 cut(s) 1229
HhaI GCGC 1 cut(s) 637
Hin1II CATG 8 cut(s) 251, 305, 403, 512, 886, 899, 928, 1222
Hin6I GCGC 1 cut(s) 635
HinP1I GCGC 1 cut(s) 635
HindIII AAGCTT 2 cut(s) 644, 1010
HinfI GANTC 2 cut(s) 527, 910
HpaII CCGG 3 cut(s) 145, 288, 568
HphI GGTGA 1 cut(s) 793
Hpy166II GTNNAC 2 cut(s) 27, 1114
Hpy188I TCNGA 6 cut(s) 58, 433, 501, 744, 965, 1191
Hpy8I GTNNAC 2 cut(s) 27, 1114
HpyCH4III ACNGT 4 cut(s) 209, 234, 1165, 1263
HpyCH4IV ACGT 1 cut(s) 1195
HpyCH4V TGCA 4 cut(s) 197, 697, 1004, 1252
HpyF10VI GCNNNNNNNGC 3 cut(s) 643, 727, 869
HpyF3I CTNAG 4 cut(s) 483, 500, 676, 792
HpySE526I ACGT 1 cut(s) 1195
Hsp92II CATG 8 cut(s) 251, 305, 403, 512, 886, 899, 928, 1222
HspAI GCGC 1 cut(s) 635
Kzo9I GATC 7 cut(s) 39, 53, 129, 703, 749, 1084, 1099
LmnI GCTCC 2 cut(s) 77, 677
Lsp1109I GCAGC 2 cut(s) 889, 989
MaeI CTAG 3 cut(s) 159, 215, 578
MaeII ACGT 1 cut(s) 1195
MalI GATC 7 cut(s) 41, 55, 131, 705, 751, 1086, 1101
MboI GATC 7 cut(s) 39, 53, 129, 703, 749, 1084, 1099
MboII GAAGA 8 cut(s) 71, 522, 713, 727, 796, 913, 995, 1085
MflI RGATCY 2 cut(s) 53, 1099
MhlI GDGCHC 2 cut(s) 865, 1058
MlsI TGGCCA 1 cut(s) 1158
MluCI AATT 8 cut(s) 182, 305, 388, 504, 600, 612, 772, 1038
MluNI TGGCCA 1 cut(s) 1158
MlyI GAGTC 1 cut(s) 904
MmeI TCCRAC 2 cut(s) 142, 268
Mox20I TGGCCA 1 cut(s) 1158
MscI TGGCCA 1 cut(s) 1158
MseI TTAA 1 cut(s) 867
Msp20I TGGCCA 1 cut(s) 1158
MspA1I CMGCKG 1 cut(s) 260
MspI CCGG 3 cut(s) 145, 288, 568
MspR9I CCNGG 2 cut(s) 145, 569
MwoI GCNNNNNNNGC 3 cut(s) 643, 727, 869
NciI CCSGG 2 cut(s) 145, 569
NdeII GATC 7 cut(s) 39, 53, 129, 703, 749, 1084, 1099
NlaIII CATG 8 cut(s) 251, 305, 403, 512, 886, 899, 928, 1222
NspI RCATGY 1 cut(s) 1222
PagI TCATGA 5 cut(s) 301, 399, 508, 882, 924
PciI ACATGT 1 cut(s) 1218
PcsI WCGNNNNNNNCGW 1 cut(s) 1201
PfeI GAWTC 1 cut(s) 527
PfoI TCCNGGA 1 cut(s) 567
PinAI ACCGGT 1 cut(s) 287
PkrI GCNGC 3 cut(s) 904, 933, 979
PleI GAGTC 1 cut(s) 904
PpsI GAGTC 1 cut(s) 904
PscI ACATGT 1 cut(s) 1218
Psp124BI GAGCTC 1 cut(s) 1058
Psp1406I AACGTT 1 cut(s) 1195
PspFI CCCAGC 1 cut(s) 342
PspPI GGNCC 1 cut(s) 218
PsrI GAACNNNNNNTAC 2 cut(s) 10, 42
PsuI RGATCY 2 cut(s) 53, 1099
PvuII CAGCTG 1 cut(s) 260
RsaI GTAC 3 cut(s) 211, 224, 332
RsaNI GTAC 3 cut(s) 210, 223, 331
SacI GAGCTC 1 cut(s) 1058
SaqAI TTAA 1 cut(s) 867
SatI GCNGC 3 cut(s) 903, 932, 978
Sau3AI GATC 7 cut(s) 39, 53, 129, 703, 749, 1084, 1099
Sau96I GGNCC 1 cut(s) 218
ScaI AGTACT 1 cut(s) 211
SchI GAGTC 1 cut(s) 904
ScrFI CCNGG 2 cut(s) 145, 569
SduI GDGCHC 2 cut(s) 865, 1058
SfcI CTRYAG 1 cut(s) 444
SpeI ACTAGT 1 cut(s) 158
Sse9I AATT 8 cut(s) 182, 305, 388, 504, 600, 612, 772, 1038
SsiI CCGC 2 cut(s) 832, 932
SspI AATATT 1 cut(s) 1256
SspMI CTAG 3 cut(s) 159, 215, 578
SstI GAGCTC 1 cut(s) 1058
StyD4I CCNGG 2 cut(s) 143, 567
StyI CCWWGG 1 cut(s) 1177
TaaI ACNGT 4 cut(s) 209, 234, 1165, 1263
TaiI ACGT 1 cut(s) 1198
TaqI TCGA 5 cut(s) 38, 272, 351, 598, 1096
TasI AATT 8 cut(s) 182, 305, 388, 504, 600, 612, 772, 1038
TatI WGTACW 2 cut(s) 209, 330
TauI GCSGC 1 cut(s) 934
TfiI GAWTC 1 cut(s) 527
Tru1I TTAA 1 cut(s) 867
Tru9I TTAA 1 cut(s) 867
TscAI CASTG 4 cut(s) 239, 460, 540, 657
TseI GCWGC 2 cut(s) 902, 977
TspRI CASTG 4 cut(s) 239, 460, 540, 657
XapI RAATTY 4 cut(s) 305, 504, 772, 1038
XceI RCATGY 1 cut(s) 1222
XmiI GTMKAC 1 cut(s) 26
XspI CTAG 3 cut(s) 159, 215, 578
ZrmI AGTACT 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.