FvH4_6g29301

Calcineurin subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002587
Physical Location & Seq
Reverse (-)
22593608 .. 22596838
3231 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g29301.t2

Sequence Viewer

Length: 528 bp
ATGGGGAGTACCTCATCGATGCTAACACAGTATGACATAGAAGAAGTACAGGAGCACTGCCATAATCTTTTTTCCCAGCAAGAAATTGTTTCCTTGTACAAACGATTTTGTCAGCTTGATCGGAATGCAAAGGGTTTCATCTCAGCTGACGAATTCTTATCCGTACCTGAGTTAGCAATGAATCCAATCTCTCAGAGGCTGCTTAAAATGGTGGAAGGTTTGAACTTTAAGGACTTTGTGGCTTTCTTGTCAGCGTTTAGTGCTAGAGCTACTATGCAGCAGAAAATGGAATTTATCTTCAAGGTATATGATTCAGACCGCAATGGAAAGGTTTCTTTCAATGATATATTAGAAGTACTCCGGGATTTGTCTGGTTCATTTCTGTCTGACGAGCAAAGAGAGAAAGTTTTGACCCAAGTCTTGCAAGAATCAGGATATTCAAGGGGATCTTATTTGACATTAGATGACTTCAGCAAGGTTCTTGGCGGTCATGGCCTAAAAATGGAGGTTGAGGTTCCAATTGACTGA

Protein Analysis

176

Amino Acids

19.96

Weight (kDa)

4.92

Isoelectric Point (pI)

36.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 94 - 160 8e-07 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 319, 486
AclWI GGATC 1 cut(s) 454
AcsI RAATTY 2 cut(s) 152, 290
AcuI CTGAAG 1 cut(s) 454
AfaI GTAC 5 cut(s) 10, 48, 98, 165, 357
AfiI CCNNNNNNNGG 1 cut(s) 502
AgsI TTSAA 4 cut(s) 223, 301, 340, 441
AluBI AGCT 3 cut(s) 115, 146, 269
AluI AGCT 3 cut(s) 115, 146, 269
Alw21I GWGCWC 1 cut(s) 57
AlwI GGATC 1 cut(s) 454
AlwNI CAGNNNCTG 1 cut(s) 199
AoxI GGCC 1 cut(s) 493
ApeKI GCWGC 2 cut(s) 199, 277
ApoI RAATTY 2 cut(s) 152, 290
Asp700I GAANNNNTTC 1 cut(s) 331
AsuC2I CCSGG 1 cut(s) 362
Bbv12I GWGCWC 1 cut(s) 57
BbvI GCAGC 2 cut(s) 186, 289
BcnI CCSGG 1 cut(s) 362
BfaI CTAG 1 cut(s) 264
BisI GCNGC 2 cut(s) 200, 278
BlsI GCNGC 2 cut(s) 201, 279
BmcAI AGTACT 1 cut(s) 357
Bme1390I CCNGG 1 cut(s) 362
BmiI GGNNCC 1 cut(s) 516
BmrFI CCNGG 1 cut(s) 362
BmsI GCATC 1 cut(s) 9
BoxI GACNNNNGTC 1 cut(s) 416
BpuMI CCSGG 1 cut(s) 362
Bsa29I ATCGAT 1 cut(s) 17
Bsc4I CCNNNNNNNGG 1 cut(s) 502
Bse3DI GCAATG 2 cut(s) 183, 328
BseCI ATCGAT 1 cut(s) 17
BseLI CCNNNNNNNGG 1 cut(s) 502
BseMI GCAATG 2 cut(s) 183, 328
BseMII CTCAG 3 cut(s) 156, 159, 206
BseXI GCAGC 2 cut(s) 186, 289
BseYI CCCAGC 1 cut(s) 75
BshFI GGCC 1 cut(s) 495
BshVI ATCGAT 1 cut(s) 17
BsiHKAI GWGCWC 1 cut(s) 57
BsiSI CCGG 1 cut(s) 361
BslI CCNNNNNNNGG 1 cut(s) 502
BsmI GAATGC 1 cut(s) 130
BsnI GGCC 1 cut(s) 495
Bsp1286I GDGCHC 1 cut(s) 57
Bsp1407I TGTACA 1 cut(s) 96
Bsp143I GATC 2 cut(s) 118, 446
BspACI CCGC 2 cut(s) 319, 486
BspANI GGCC 1 cut(s) 495
BspCNI CTCAG 3 cut(s) 155, 160, 205
BspDI ATCGAT 1 cut(s) 17
BspLI GGNNCC 1 cut(s) 516
BspPI GGATC 1 cut(s) 454
BsrDI GCAATG 2 cut(s) 183, 328
BsrGI TGTACA 1 cut(s) 96
BssMI GATC 2 cut(s) 118, 446
Bst4CI ACNGT 1 cut(s) 30
BstAUI TGTACA 1 cut(s) 96
BstDEI CTNAG 3 cut(s) 142, 168, 192
BstKTI GATC 2 cut(s) 121, 449
BstMBI GATC 2 cut(s) 118, 446
BstMWI GCNNNNNNNGC 2 cut(s) 260, 492
BstPAI GACNNNNGTC 1 cut(s) 416
BstSCI CCNGG 1 cut(s) 360
BstV1I GCAGC 2 cut(s) 186, 289
BstX2I RGATCY 1 cut(s) 446
BstYI RGATCY 1 cut(s) 446
Bsu15I ATCGAT 1 cut(s) 17
BsuRI GGCC 1 cut(s) 495
BsuTUI ATCGAT 1 cut(s) 17
BtsI GCAGTG 1 cut(s) 55
BtsIMutI CAGTG 1 cut(s) 55
CaiI CAGNNNCTG 1 cut(s) 199
ClaI ATCGAT 1 cut(s) 17
Csp6I GTAC 5 cut(s) 9, 47, 97, 164, 356
CviAII CATG 1 cut(s) 491
CviJI RGCY 6 cut(s) 115, 146, 199, 242, 269, 495
CviKI_1 RGCY 6 cut(s) 115, 146, 199, 242, 269, 495
CviQI GTAC 5 cut(s) 9, 47, 97, 164, 356
DdeI CTNAG 3 cut(s) 142, 168, 192
DpnI GATC 2 cut(s) 120, 448
DpnII GATC 2 cut(s) 118, 446
Eco57I CTGAAG 1 cut(s) 454
EcoRI GAATTC 1 cut(s) 152
FaeI CATG 1 cut(s) 494
FaiI YATR 8 cut(s) 33, 38, 63, 275, 307, 309, 347, 492
FalI AAGNNNNNCTT 2 cut(s) 433, 465
FatI CATG 1 cut(s) 490
Fnu4HI GCNGC 2 cut(s) 200, 278
Fsp4HI GCNGC 2 cut(s) 200, 278
FspBI CTAG 1 cut(s) 264
GluI GCNGC 2 cut(s) 200, 278
GsaI CCCAGC 1 cut(s) 79
HaeIII GGCC 1 cut(s) 495
HapII CCGG 1 cut(s) 361
Hin1II CATG 1 cut(s) 494
HinfI GANTC 3 cut(s) 181, 311, 428
HpaII CCGG 1 cut(s) 361
Hpy188I TCNGA 4 cut(s) 123, 195, 316, 388
Hpy188III TCNNGA 1 cut(s) 432
HpyAV CCTTC 1 cut(s) 209
HpyCH4III ACNGT 1 cut(s) 30
HpyCH4V TGCA 3 cut(s) 128, 277, 424
HpyF10VI GCNNNNNNNGC 2 cut(s) 260, 492
HpyF3I CTNAG 3 cut(s) 142, 168, 192
Hsp92II CATG 1 cut(s) 494
Kzo9I GATC 2 cut(s) 118, 446
LmnI GCTCC 1 cut(s) 52
LpnPI CCDG 6 cut(s) 35, 89, 180, 357, 374, 417
Lsp1109I GCAGC 2 cut(s) 186, 289
LweI GCATC 1 cut(s) 9
MaeI CTAG 1 cut(s) 264
MalI GATC 2 cut(s) 120, 448
MboI GATC 2 cut(s) 118, 446
MboII GAAGA 2 cut(s) 53, 289
MfeI CAATTG 1 cut(s) 519
MflI RGATCY 1 cut(s) 446
MhlI GDGCHC 1 cut(s) 57
MluCI AATT 4 cut(s) 84, 152, 290, 519
MnlI CCTC 4 cut(s) 22, 189, 499, 505
MroXI GAANNNNTTC 1 cut(s) 331
MseI TTAA 2 cut(s) 204, 228
MspA1I CMGCKG 1 cut(s) 146
MspI CCGG 1 cut(s) 361
MspR9I CCNGG 1 cut(s) 362
MunI CAATTG 1 cut(s) 519
Mva1269I GAATGC 1 cut(s) 130
MwoI GCNNNNNNNGC 2 cut(s) 260, 492
NciI CCSGG 1 cut(s) 362
NdeII GATC 2 cut(s) 118, 446
NlaIII CATG 1 cut(s) 494
NlaIV GGNNCC 1 cut(s) 516
PctI GAATGC 1 cut(s) 130
PdmI GAANNNNTTC 1 cut(s) 331
PfeI GAWTC 3 cut(s) 181, 311, 428
PfoI TCCNGGA 1 cut(s) 360
PkrI GCNGC 2 cut(s) 201, 279
PshAI GACNNNNGTC 1 cut(s) 416
PspFI CCCAGC 1 cut(s) 75
PspN4I GGNNCC 1 cut(s) 516
PstNI CAGNNNCTG 1 cut(s) 199
PsuI RGATCY 1 cut(s) 446
PvuII CAGCTG 1 cut(s) 146
RsaI GTAC 5 cut(s) 10, 48, 98, 165, 357
RsaNI GTAC 5 cut(s) 9, 47, 97, 164, 356
SaqAI TTAA 2 cut(s) 204, 228
SatI GCNGC 2 cut(s) 200, 278
Sau3AI GATC 2 cut(s) 118, 446
ScaI AGTACT 1 cut(s) 357
ScrFI CCNGG 1 cut(s) 362
SduI GDGCHC 1 cut(s) 57
SfaNI GCATC 1 cut(s) 9
Sse9I AATT 4 cut(s) 84, 152, 290, 519
SsiI CCGC 2 cut(s) 319, 486
SspMI CTAG 1 cut(s) 264
StyD4I CCNGG 1 cut(s) 360
TaaI ACNGT 1 cut(s) 30
TaqI TCGA 1 cut(s) 17
TasI AATT 4 cut(s) 84, 152, 290, 519
TatI WGTACW 3 cut(s) 46, 96, 355
TfiI GAWTC 3 cut(s) 181, 311, 428
Tru1I TTAA 2 cut(s) 204, 228
Tru9I TTAA 2 cut(s) 204, 228
TscAI CASTG 1 cut(s) 62
TseI GCWGC 2 cut(s) 199, 277
TspDTI ATGAA 3 cut(s) 127, 194, 366
TspGWI ACGGA 1 cut(s) 151
TspRI CASTG 1 cut(s) 62
XapI RAATTY 2 cut(s) 152, 290
XmnI GAANNNNTTC 1 cut(s) 331
XspI CTAG 1 cut(s) 264
ZrmI AGTACT 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.