Rh2DG009000

Calcineurin subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0002587
Physical Location & Seq
Reverse (-)
527751 .. 530735
2985 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG009000.1

Sequence Viewer

Length: 528 bp
ATGGGCAACACATCCTCCATGCTCACTCAGTACGACATCGAAGAAGTTCAGCAACACTGCAATCACACGTTTTCGCAGCAGGAGATAGTTTCTCTGTACGAGAGGTTCTGTCAGCTCGATCGCAGCGGCGGAGGTTTCATCTCCGCCGACGAGTTCTTGTCCGTACCTGAATTCGCCGTCAATCCTCTCTCTCAGAGATTGCTGAAGGTTTTGGATGGATTGAACTTCAAGGAATTTGTTGCATTCTTGTCAGCATTCAGTTCTCGTGCAAGCTTGCAGCAAAAGATTGAGTTTATCTTCAAGGTATATGATTCCGATCAGAATGGGAAAGTCACGTTCAATGACATGCTGGATGTTTTACGGGATTTGACGGGGCAGTTCATATCTGAGCAACAGAGGGAGCAAGTATTGACCCATGTCCTCGAGGAATCAGGCTATACAAAGGATTCTTCTCTAACCATATCTGACTTTGTGAAGACTCTTGGAAACTCAGCTTTGAAGATGGAGGTTGAGGTTCCTGTGGATTAA

Protein Analysis

175

Amino Acids

19.84

Weight (kDa)

4.58

Isoelectric Point (pI)

38.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 94 - 159 2.4e-07 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 422
AciI CCGC 3 cut(s) 126, 129, 144
AcsI RAATTY 2 cut(s) 170, 233
AcuI CTGAAG 1 cut(s) 224
AfaI GTAC 3 cut(s) 32, 98, 165
AflIII ACRYGT 1 cut(s) 66
AgsI TTSAA 5 cut(s) 223, 229, 301, 340, 499
AluBI AGCT 3 cut(s) 115, 273, 494
AluI AGCT 3 cut(s) 115, 273, 494
Ama87I CYCGRG 1 cut(s) 422
ApeKI GCWGC 3 cut(s) 76, 123, 277
ApoI RAATTY 2 cut(s) 170, 233
Asp700I GAANNNNTTC 1 cut(s) 45
AvaI CYCGRG 1 cut(s) 422
BauI CACGAG 1 cut(s) 264
BbsI GAAGAC 1 cut(s) 482
BbvI GCAGC 3 cut(s) 88, 135, 289
BccI CCATC 2 cut(s) 209, 496
BceAI ACGGC 1 cut(s) 161
BisI GCNGC 4 cut(s) 77, 124, 127, 278
BlsI GCNGC 4 cut(s) 78, 125, 128, 279
BmeT110I CYCGRG 1 cut(s) 422
BmiI GGNNCC 1 cut(s) 516
BoxI GACNNNNGTC 1 cut(s) 416
BpiI GAAGAC 1 cut(s) 482
BsaBI GATNNNNATC 1 cut(s) 315
BsaXI ACNNNNNCTCC 1 cut(s) 29
Bse8I GATNNNNATC 1 cut(s) 315
BseGI GGATG 3 cut(s) 11, 220, 358
BseJI GATNNNNATC 1 cut(s) 315
BseMII CTCAG 4 cut(s) 41, 206, 378, 504
BseXI GCAGC 3 cut(s) 88, 135, 289
Bsh1285I CGRYCG 1 cut(s) 121
BsiEI CGRYCG 1 cut(s) 121
BsiHKCI CYCGRG 1 cut(s) 422
BsmI GAATGC 2 cut(s) 242, 254
BsoBI CYCGRG 1 cut(s) 422
Bsp143I GATC 2 cut(s) 118, 316
BspACI CCGC 3 cut(s) 126, 129, 144
BspCNI CTCAG 4 cut(s) 40, 205, 379, 503
BspLI GGNNCC 1 cut(s) 516
BssMI GATC 2 cut(s) 118, 316
BssSI CACGAG 1 cut(s) 264
Bst2BI CACGAG 1 cut(s) 264
BstC8I GCNNGC 2 cut(s) 271, 275
BstDEI CTNAG 4 cut(s) 27, 192, 387, 490
BstF5I GGATG 3 cut(s) 11, 220, 358
BstKTI GATC 2 cut(s) 121, 319
BstMBI GATC 2 cut(s) 118, 316
BstMCI CGRYCG 1 cut(s) 121
BstNSI RCATGY 1 cut(s) 349
BstPAI GACNNNNGTC 1 cut(s) 416
BstV1I GCAGC 3 cut(s) 88, 135, 289
BstV2I GAAGAC 1 cut(s) 482
BtsCI GGATG 3 cut(s) 11, 220, 358
BtsI GCAGTG 1 cut(s) 55
BtsIMutI CAGTG 1 cut(s) 55
Cac8I GCNNGC 2 cut(s) 271, 275
Csp6I GTAC 3 cut(s) 31, 97, 164
CviAII CATG 3 cut(s) 19, 346, 416
CviJI RGCY 4 cut(s) 115, 273, 435, 494
CviKI_1 RGCY 4 cut(s) 115, 273, 435, 494
CviQI GTAC 3 cut(s) 31, 97, 164
DdeI CTNAG 4 cut(s) 27, 192, 387, 490
DpnI GATC 2 cut(s) 120, 318
DpnII GATC 2 cut(s) 118, 316
EciI GGCGGA 2 cut(s) 133, 144
Eco57I CTGAAG 1 cut(s) 224
Eco88I CYCGRG 1 cut(s) 422
EcoRI GAATTC 1 cut(s) 170
FaeI CATG 3 cut(s) 22, 349, 419
FaiI YATR 8 cut(s) 20, 307, 309, 347, 383, 417, 438, 461
FatI CATG 3 cut(s) 18, 345, 415
Fnu4HI GCNGC 4 cut(s) 77, 124, 127, 278
FokI GGATG 2 cut(s) 227, 365
Fsp4HI GCNGC 4 cut(s) 77, 124, 127, 278
GluI GCNGC 4 cut(s) 77, 124, 127, 278
Hin1II CATG 3 cut(s) 22, 349, 419
HindIII AAGCTT 1 cut(s) 271
HinfI GANTC 4 cut(s) 311, 428, 446, 478
Hpy188I TCNGA 5 cut(s) 195, 316, 321, 388, 466
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 1 cut(s) 199
HpyCH4IV ACGT 2 cut(s) 68, 335
HpyCH4V TGCA 4 cut(s) 60, 242, 269, 277
HpyF3I CTNAG 4 cut(s) 27, 192, 387, 490
HpySE526I ACGT 2 cut(s) 68, 335
Hsp92II CATG 3 cut(s) 22, 349, 419
Kzo9I GATC 2 cut(s) 118, 316
LmnI GCTCC 1 cut(s) 400
LpnPI CCDG 4 cut(s) 65, 180, 335, 417
Lsp1109I GCAGC 3 cut(s) 88, 135, 289
MaeII ACGT 2 cut(s) 68, 335
MaeIII GTNAC 1 cut(s) 331
MalI GATC 2 cut(s) 120, 318
MboI GATC 2 cut(s) 118, 316
MboII GAAGA 5 cut(s) 53, 289, 441, 487, 511
MluCI AATT 2 cut(s) 170, 233
MlyI GAGTC 1 cut(s) 472
MnlI CCTC 9 cut(s) 25, 96, 125, 195, 390, 418, 431, 499, 505
MroXI GAANNNNTTC 1 cut(s) 45
MseI TTAA 1 cut(s) 526
MspA1I CMGCKG 1 cut(s) 126
Mva1269I GAATGC 2 cut(s) 242, 254
NdeII GATC 2 cut(s) 118, 316
NlaIII CATG 3 cut(s) 22, 349, 419
NlaIV GGNNCC 1 cut(s) 516
NmuCI GTSAC 1 cut(s) 331
NspI RCATGY 1 cut(s) 349
PaeR7I CTCGAG 1 cut(s) 422
PctI GAATGC 2 cut(s) 242, 254
PdmI GAANNNNTTC 1 cut(s) 45
PfeI GAWTC 3 cut(s) 311, 428, 446
PkrI GCNGC 4 cut(s) 78, 125, 128, 279
Ple19I CGATCG 1 cut(s) 121
PleI GAGTC 1 cut(s) 472
PpsI GAGTC 1 cut(s) 472
PshAI GACNNNNGTC 1 cut(s) 416
PspN4I GGNNCC 1 cut(s) 516
PspXI VCTCGAGB 1 cut(s) 422
PsrI GAACNNNNNNTAC 2 cut(s) 89, 121
PvuI CGATCG 1 cut(s) 121
RsaI GTAC 3 cut(s) 32, 98, 165
RsaNI GTAC 3 cut(s) 31, 97, 164
SaqAI TTAA 1 cut(s) 526
SatI GCNGC 4 cut(s) 77, 124, 127, 278
Sau3AI GATC 2 cut(s) 118, 316
SchI GAGTC 1 cut(s) 472
Sfr274I CTCGAG 1 cut(s) 422
SlaI CTCGAG 1 cut(s) 422
SmlI CTYRAG 1 cut(s) 422
SmoI CTYRAG 1 cut(s) 422
Sse9I AATT 2 cut(s) 170, 233
SsiI CCGC 3 cut(s) 126, 129, 144
TaiI ACGT 2 cut(s) 71, 338
TaqI TCGA 3 cut(s) 39, 117, 423
TasI AATT 2 cut(s) 170, 233
TauI GCSGC 1 cut(s) 129
TfiI GAWTC 3 cut(s) 311, 428, 446
Tru1I TTAA 1 cut(s) 526
Tru9I TTAA 1 cut(s) 526
TscAI CASTG 1 cut(s) 62
TseFI GTSAC 1 cut(s) 331
TseI GCWGC 3 cut(s) 76, 123, 277
Tsp45I GTSAC 1 cut(s) 331
TspDTI ATGAA 2 cut(s) 127, 370
TspGWI ACGGA 1 cut(s) 151
TspRI CASTG 1 cut(s) 62
XapI RAATTY 2 cut(s) 170, 233
XceI RCATGY 1 cut(s) 349
XhoI CTCGAG 1 cut(s) 422
XmnI GAANNNNTTC 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.