Rw2G030150

Calcineurin subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002587
Physical Location & Seq
Reverse (-)
49273298 .. 49277386
4089 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G030150.1

Sequence Viewer

Length: 528 bp
ATGGGAAGTACCTCATCGATGCTAACTCAGTATGACATTGAAGAAGTACAGGAGCACTGCCAGAATCTATTTTCCCAGCAAGAAATTGTATCCTTGTATAAAAGATTTTGTCAGCTGGATCGGAATGCAAAGGGTTTCATCTCATCTGATGAATTCTTATCAGTACCCGAGTTAGCAACGAATCCAATATCTCAGAGGCTGCTTAAGATGGTGGAAGGTTTGAACTTTAAGGACTTTGTGGCTTTCTTGTCTGCGTTTAGTGCTAGGGCTACTATGCAGCAGAAAATTGAATTTATTTTCAAGGTATATGATTCAGACCGCAATGGAAAGGTGTCTTTCAATGATATATTAGAAGTGCTCCGGGATATGTCTGGTTCATTTATGTCTGACGAGCAAAGAGAGAAAGTGTTGACCCAAGTCTTGCAAGAATCAGGATATTCAAGGGGATCTTATTTGACATTAGATGACTTCAGTAAGGTTCTTGGCGGTCATGGCCTAAAAATGGAGGTTGAGGTGCCAATTGACTAA

Protein Analysis

175

Amino Acids

19.96

Weight (kDa)

4.83

Isoelectric Point (pI)

38.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 94 - 160 2.3e-06 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 514
AciI CCGC 2 cut(s) 319, 486
AclWI GGATC 2 cut(s) 126, 454
AcsI RAATTY 2 cut(s) 152, 290
AcuI CTGAAG 1 cut(s) 454
AfaI GTAC 3 cut(s) 10, 48, 165
AfiI CCNNNNNNNGG 1 cut(s) 502
AflII CTTAAG 1 cut(s) 203
AgsI TTSAA 6 cut(s) 41, 223, 290, 301, 340, 441
AluBI AGCT 1 cut(s) 115
AluI AGCT 1 cut(s) 115
Alw21I GWGCWC 2 cut(s) 57, 360
AlwI GGATC 2 cut(s) 126, 454
AlwNI CAGNNNCTG 1 cut(s) 199
Ama87I CYCGRG 1 cut(s) 167
AoxI GGCC 1 cut(s) 493
ApeKI GCWGC 2 cut(s) 199, 277
ApoI RAATTY 2 cut(s) 152, 290
AsuC2I CCSGG 1 cut(s) 362
AvaI CYCGRG 1 cut(s) 167
BanI GGYRCC 1 cut(s) 514
Bbv12I GWGCWC 2 cut(s) 57, 360
BbvI GCAGC 2 cut(s) 186, 289
BccI CCATC 1 cut(s) 202
BciVI GTATCC 1 cut(s) 100
BcnI CCSGG 1 cut(s) 362
BfaI CTAG 1 cut(s) 264
BfrI CTTAAG 1 cut(s) 203
BfuI GTATCC 1 cut(s) 100
BisI GCNGC 2 cut(s) 200, 278
BlsI GCNGC 2 cut(s) 201, 279
Bme1390I CCNGG 1 cut(s) 362
BmeT110I CYCGRG 1 cut(s) 167
BmiI GGNNCC 1 cut(s) 516
BmrFI CCNGG 1 cut(s) 362
BmsI GCATC 1 cut(s) 9
BoxI GACNNNNGTC 1 cut(s) 416
BpuMI CCSGG 1 cut(s) 362
Bsa29I ATCGAT 1 cut(s) 17
Bsc4I CCNNNNNNNGG 1 cut(s) 502
Bse3DI GCAATG 1 cut(s) 328
BseCI ATCGAT 1 cut(s) 17
BseLI CCNNNNNNNGG 1 cut(s) 502
BseMI GCAATG 1 cut(s) 328
BseMII CTCAG 2 cut(s) 41, 206
BseXI GCAGC 2 cut(s) 186, 289
BseYI CCCAGC 1 cut(s) 75
BshFI GGCC 1 cut(s) 495
BshNI GGYRCC 1 cut(s) 514
BshVI ATCGAT 1 cut(s) 17
BsiHKAI GWGCWC 2 cut(s) 57, 360
BsiHKCI CYCGRG 1 cut(s) 167
BsiSI CCGG 1 cut(s) 361
BslI CCNNNNNNNGG 1 cut(s) 502
BsmI GAATGC 1 cut(s) 130
BsnI GGCC 1 cut(s) 495
BsoBI CYCGRG 1 cut(s) 167
Bsp1286I GDGCHC 2 cut(s) 57, 360
Bsp143I GATC 2 cut(s) 118, 446
BspACI CCGC 2 cut(s) 319, 486
BspANI GGCC 1 cut(s) 495
BspCNI CTCAG 2 cut(s) 40, 205
BspDI ATCGAT 1 cut(s) 17
BspLI GGNNCC 1 cut(s) 516
BspPI GGATC 2 cut(s) 126, 454
BspT107I GGYRCC 1 cut(s) 514
BspTI CTTAAG 1 cut(s) 203
BsrDI GCAATG 1 cut(s) 328
BssMI GATC 2 cut(s) 118, 446
BstAFI CTTAAG 1 cut(s) 203
BstDEI CTNAG 2 cut(s) 27, 192
BstKTI GATC 2 cut(s) 121, 449
BstMBI GATC 2 cut(s) 118, 446
BstMWI GCNNNNNNNGC 2 cut(s) 260, 492
BstPAI GACNNNNGTC 1 cut(s) 416
BstSCI CCNGG 1 cut(s) 360
BstV1I GCAGC 2 cut(s) 186, 289
BstX2I RGATCY 1 cut(s) 446
BstYI RGATCY 1 cut(s) 446
Bsu15I ATCGAT 1 cut(s) 17
BsuI GTATCC 1 cut(s) 100
BsuRI GGCC 1 cut(s) 495
BsuTUI ATCGAT 1 cut(s) 17
BtsI GCAGTG 1 cut(s) 55
BtsIMutI CAGTG 1 cut(s) 55
CaiI CAGNNNCTG 1 cut(s) 199
ClaI ATCGAT 1 cut(s) 17
Csp6I GTAC 3 cut(s) 9, 47, 164
CviAII CATG 1 cut(s) 491
CviJI RGCY 5 cut(s) 115, 199, 242, 269, 495
CviKI_1 RGCY 5 cut(s) 115, 199, 242, 269, 495
CviQI GTAC 3 cut(s) 9, 47, 164
DdeI CTNAG 2 cut(s) 27, 192
DpnI GATC 2 cut(s) 120, 448
DpnII GATC 2 cut(s) 118, 446
Eco57I CTGAAG 1 cut(s) 454
Eco88I CYCGRG 1 cut(s) 167
EcoRI GAATTC 1 cut(s) 152
FaeI CATG 1 cut(s) 494
FaiI YATR 9 cut(s) 33, 99, 275, 307, 309, 347, 368, 383, 492
FalI AAGNNNNNCTT 2 cut(s) 433, 465
FatI CATG 1 cut(s) 490
Fnu4HI GCNGC 2 cut(s) 200, 278
Fsp4HI GCNGC 2 cut(s) 200, 278
FspBI CTAG 1 cut(s) 264
GluI GCNGC 2 cut(s) 200, 278
GsaI CCCAGC 1 cut(s) 79
HaeIII GGCC 1 cut(s) 495
HapII CCGG 1 cut(s) 361
Hin1II CATG 1 cut(s) 494
HincII GTYRAC 1 cut(s) 411
HindII GTYRAC 1 cut(s) 411
HinfI GANTC 4 cut(s) 64, 181, 311, 428
HpaII CCGG 1 cut(s) 361
Hpy166II GTNNAC 1 cut(s) 411
Hpy188I TCNGA 5 cut(s) 123, 148, 195, 316, 388
Hpy188III TCNNGA 1 cut(s) 432
Hpy8I GTNNAC 1 cut(s) 411
HpyAV CCTTC 1 cut(s) 209
HpyCH4V TGCA 3 cut(s) 128, 277, 424
HpyF10VI GCNNNNNNNGC 2 cut(s) 260, 492
HpyF3I CTNAG 2 cut(s) 27, 192
Hsp92II CATG 1 cut(s) 494
Kzo9I GATC 2 cut(s) 118, 446
LmnI GCTCC 2 cut(s) 52, 363
LpnPI CCDG 7 cut(s) 35, 74, 89, 101, 357, 374, 417
Lsp1109I GCAGC 2 cut(s) 186, 289
LweI GCATC 1 cut(s) 9
MaeI CTAG 1 cut(s) 264
MalI GATC 2 cut(s) 120, 448
MboI GATC 2 cut(s) 118, 446
MboII GAAGA 1 cut(s) 53
MfeI CAATTG 1 cut(s) 519
MflI RGATCY 1 cut(s) 446
MhlI GDGCHC 2 cut(s) 57, 360
MluCI AATT 5 cut(s) 84, 152, 285, 290, 519
MnlI CCTC 4 cut(s) 22, 189, 499, 505
MseI TTAA 2 cut(s) 204, 228
MspA1I CMGCKG 1 cut(s) 115
MspCI CTTAAG 1 cut(s) 203
MspI CCGG 1 cut(s) 361
MspR9I CCNGG 1 cut(s) 362
MunI CAATTG 1 cut(s) 519
Mva1269I GAATGC 1 cut(s) 130
MwoI GCNNNNNNNGC 2 cut(s) 260, 492
NciI CCSGG 1 cut(s) 362
NdeII GATC 2 cut(s) 118, 446
NlaIII CATG 1 cut(s) 494
NlaIV GGNNCC 1 cut(s) 516
PctI GAATGC 1 cut(s) 130
PfeI GAWTC 4 cut(s) 64, 181, 311, 428
PfoI TCCNGGA 1 cut(s) 360
PkrI GCNGC 2 cut(s) 201, 279
PshAI GACNNNNGTC 1 cut(s) 416
PspFI CCCAGC 1 cut(s) 75
PspN4I GGNNCC 1 cut(s) 516
PstNI CAGNNNCTG 1 cut(s) 199
PsuI RGATCY 1 cut(s) 446
PvuII CAGCTG 1 cut(s) 115
RsaI GTAC 3 cut(s) 10, 48, 165
RsaNI GTAC 3 cut(s) 9, 47, 164
SaqAI TTAA 2 cut(s) 204, 228
SatI GCNGC 2 cut(s) 200, 278
Sau3AI GATC 2 cut(s) 118, 446
ScrFI CCNGG 1 cut(s) 362
SduI GDGCHC 2 cut(s) 57, 360
SetI ASST 8 cut(s) 14, 117, 220, 306, 333, 480, 510, 516
SfaNI GCATC 1 cut(s) 9
SmlI CTYRAG 1 cut(s) 203
SmoI CTYRAG 1 cut(s) 203
Sse9I AATT 5 cut(s) 84, 152, 285, 290, 519
SsiI CCGC 2 cut(s) 319, 486
SspMI CTAG 1 cut(s) 264
StyD4I CCNGG 1 cut(s) 360
TaqI TCGA 1 cut(s) 17
TasI AATT 5 cut(s) 84, 152, 285, 290, 519
TatI WGTACW 1 cut(s) 46
TfiI GAWTC 4 cut(s) 64, 181, 311, 428
Tru1I TTAA 2 cut(s) 204, 228
Tru9I TTAA 2 cut(s) 204, 228
TscAI CASTG 1 cut(s) 62
TseI GCWGC 2 cut(s) 199, 277
TspDTI ATGAA 3 cut(s) 127, 165, 366
TspRI CASTG 1 cut(s) 62
Vha464I CTTAAG 1 cut(s) 203
XapI RAATTY 2 cut(s) 152, 290
XspI CTAG 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.