MD02G1005100.v1.1

Calcineurin subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0002587
Physical Location & Seq
Reverse (-)
357466 .. 359929
2464 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1005100.v1.1.491

Sequence Viewer

Length: 528 bp
ATGGGCAACACATCCTCGATGCTCACGCAGTACGACATCGAAGAAGTCCAGGAGCACTGTAACAACACATTTTCCCAGCAGGAGATAGTTTCTCTGTACCAGCGGTTCTGTCAGCTCGATCGCAGCGGCGGAGGTTTTATCTCCGCCGATGAGTTCTTGTCCGTTCCCGAATTCGCCGTCAATCCTCTCTCTCAGAGATTGCTGAAGATATTGGATGGATTGAACTTTAAGGAGTTCGTATTGTTCTTATCAGCATTCAGTTCTCGTGCAAGCTTGCAGCAAAAAATCGAGTTTATTTTTAAGGTATATGATTCAGACGGTAATGGGAAAGTCGCATTCAGCGACATGTTGGATGTTTTGCGGGATTTGACGGGGCAGTTCATATCTGAGCAACAGAGGGAGCAAGTATTGACGCATGTCCTAGAGGAATCAGGCTATACAAAGGATTCATTGTTAAGCATATCGGACTTTGTGAAGACTCTTGGCAACTCTGAATTGAAGATGGAGGTCGAGGTTCCTGTGGATTGA

Protein Analysis

176

Amino Acids

19.82

Weight (kDa)

4.48

Isoelectric Point (pI)

39.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 94 - 159 5.6e-07 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 103, 126, 129, 144, 361
AcsI RAATTY 1 cut(s) 170
AcuI CTGAAG 1 cut(s) 224
AfaI GTAC 2 cut(s) 32, 98
AflIII ACRYGT 1 cut(s) 345
AgsI TTSAA 2 cut(s) 223, 499
AjnI CCWGG 1 cut(s) 48
AluBI AGCT 2 cut(s) 115, 273
AluI AGCT 2 cut(s) 115, 273
Alw21I GWGCWC 1 cut(s) 57
ApeKI GCWGC 2 cut(s) 123, 277
ApoI RAATTY 1 cut(s) 170
ArsI GACNNNNNNTTYG 2 cut(s) 162, 194
BauI CACGAG 1 cut(s) 264
BbsI GAAGAC 1 cut(s) 482
Bbv12I GWGCWC 1 cut(s) 57
BbvI GCAGC 2 cut(s) 135, 289
BccI CCATC 2 cut(s) 209, 496
BceAI ACGGC 1 cut(s) 161
BciT130I CCWGG 1 cut(s) 50
BfaI CTAG 1 cut(s) 422
BisI GCNGC 3 cut(s) 124, 127, 278
BlsI GCNGC 3 cut(s) 125, 128, 279
Bme1390I CCNGG 1 cut(s) 50
BmiI GGNNCC 1 cut(s) 516
BmrFI CCNGG 1 cut(s) 50
BmsI GCATC 1 cut(s) 9
BoxI GACNNNNGTC 1 cut(s) 416
BpiI GAAGAC 1 cut(s) 482
BseBI CCWGG 1 cut(s) 50
BseGI GGATG 3 cut(s) 11, 220, 358
BseMII CTCAG 2 cut(s) 206, 378
BseXI GCAGC 2 cut(s) 135, 289
BseYI CCCAGC 1 cut(s) 75
Bsh1285I CGRYCG 1 cut(s) 121
BsiEI CGRYCG 1 cut(s) 121
BsiHKAI GWGCWC 1 cut(s) 57
BsmI GAATGC 2 cut(s) 254, 335
Bsp1286I GDGCHC 1 cut(s) 57
Bsp143I GATC 1 cut(s) 118
BspACI CCGC 5 cut(s) 103, 126, 129, 144, 361
BspCNI CTCAG 2 cut(s) 205, 379
BspLI GGNNCC 1 cut(s) 516
BssMI GATC 1 cut(s) 118
BssSI CACGAG 1 cut(s) 264
Bst2BI CACGAG 1 cut(s) 264
Bst2UI CCWGG 1 cut(s) 50
Bst4CI ACNGT 2 cut(s) 59, 320
BstC8I GCNNGC 2 cut(s) 271, 275
BstDEI CTNAG 2 cut(s) 192, 387
BstF5I GGATG 3 cut(s) 11, 220, 358
BstKTI GATC 1 cut(s) 121
BstMBI GATC 1 cut(s) 118
BstMCI CGRYCG 1 cut(s) 121
BstNI CCWGG 1 cut(s) 50
BstNSI RCATGY 2 cut(s) 349, 419
BstPAI GACNNNNGTC 1 cut(s) 416
BstSCI CCNGG 1 cut(s) 48
BstV1I GCAGC 2 cut(s) 135, 289
BstV2I GAAGAC 1 cut(s) 482
BtsCI GGATG 3 cut(s) 11, 220, 358
BtsIMutI CAGTG 1 cut(s) 55
Cac8I GCNNGC 2 cut(s) 271, 275
CseI GACGC 1 cut(s) 421
Csp6I GTAC 2 cut(s) 31, 97
CviAII CATG 2 cut(s) 346, 416
CviJI RGCY 3 cut(s) 115, 273, 435
CviKI_1 RGCY 3 cut(s) 115, 273, 435
CviQI GTAC 2 cut(s) 31, 97
DdeI CTNAG 2 cut(s) 192, 387
DpnI GATC 1 cut(s) 120
DpnII GATC 1 cut(s) 118
EciI GGCGGA 2 cut(s) 133, 144
Eco57I CTGAAG 1 cut(s) 224
EcoRI GAATTC 1 cut(s) 170
EcoRII CCWGG 1 cut(s) 48
FaeI CATG 2 cut(s) 349, 419
FaiI YATR 7 cut(s) 307, 309, 347, 383, 417, 438, 461
FatI CATG 2 cut(s) 345, 415
FauI CCCGC 1 cut(s) 354
Fnu4HI GCNGC 3 cut(s) 124, 127, 278
FokI GGATG 2 cut(s) 227, 365
Fsp4HI GCNGC 3 cut(s) 124, 127, 278
FspBI CTAG 1 cut(s) 422
GluI GCNGC 3 cut(s) 124, 127, 278
GsaI CCCAGC 1 cut(s) 79
HgaI GACGC 1 cut(s) 421
Hin1II CATG 2 cut(s) 349, 419
HindIII AAGCTT 1 cut(s) 271
HinfI GANTC 4 cut(s) 311, 428, 446, 478
Hpy188I TCNGA 5 cut(s) 195, 316, 388, 466, 493
Hpy188III TCNNGA 1 cut(s) 167
HpyCH4III ACNGT 2 cut(s) 59, 320
HpyCH4V TGCA 2 cut(s) 269, 277
HpyF3I CTNAG 2 cut(s) 192, 387
Hsp92II CATG 2 cut(s) 349, 419
Kzo9I GATC 1 cut(s) 118
LmnI GCTCC 2 cut(s) 52, 400
LpnPI CCDG 6 cut(s) 35, 62, 65, 89, 113, 417
Lsp1109I GCAGC 2 cut(s) 135, 289
LweI GCATC 1 cut(s) 9
MaeI CTAG 1 cut(s) 422
MaeIII GTNAC 1 cut(s) 59
MalI GATC 1 cut(s) 120
MboI GATC 1 cut(s) 118
MboII GAAGA 4 cut(s) 53, 217, 487, 511
MhlI GDGCHC 1 cut(s) 57
MluCI AATT 2 cut(s) 170, 494
MlyI GAGTC 1 cut(s) 472
MmeI TCCRAC 1 cut(s) 330
MnlI CCTC 7 cut(s) 25, 125, 195, 390, 418, 499, 505
MseI TTAA 3 cut(s) 228, 300, 455
MspA1I CMGCKG 2 cut(s) 103, 126
MspR9I CCNGG 1 cut(s) 50
Mva1269I GAATGC 2 cut(s) 254, 335
MvaI CCWGG 1 cut(s) 50
NdeII GATC 1 cut(s) 118
NlaIII CATG 2 cut(s) 349, 419
NlaIV GGNNCC 1 cut(s) 516
NspI RCATGY 2 cut(s) 349, 419
PciI ACATGT 1 cut(s) 345
PcsI WCGNNNNNNNCGW 1 cut(s) 339
PctI GAATGC 2 cut(s) 254, 335
PfeI GAWTC 3 cut(s) 311, 428, 446
PfoI TCCNGGA 1 cut(s) 48
PkrI GCNGC 3 cut(s) 125, 128, 279
Ple19I CGATCG 1 cut(s) 121
PleI GAGTC 1 cut(s) 472
PpsI GAGTC 1 cut(s) 472
PscI ACATGT 1 cut(s) 345
PshAI GACNNNNGTC 1 cut(s) 416
Psp6I CCWGG 1 cut(s) 48
PspFI CCCAGC 1 cut(s) 75
PspGI CCWGG 1 cut(s) 48
PspN4I GGNNCC 1 cut(s) 516
PsrI GAACNNNNNNTAC 2 cut(s) 89, 121
PvuI CGATCG 1 cut(s) 121
RsaI GTAC 2 cut(s) 32, 98
RsaNI GTAC 2 cut(s) 31, 97
SaqAI TTAA 3 cut(s) 228, 300, 455
SatI GCNGC 3 cut(s) 124, 127, 278
Sau3AI GATC 1 cut(s) 118
SchI GAGTC 1 cut(s) 472
ScrFI CCNGG 1 cut(s) 50
SduI GDGCHC 1 cut(s) 57
SetI ASST 6 cut(s) 117, 136, 275, 306, 510, 516
SfaNI GCATC 1 cut(s) 9
Sse9I AATT 2 cut(s) 170, 494
SsiI CCGC 5 cut(s) 103, 126, 129, 144, 361
SspMI CTAG 1 cut(s) 422
StyD4I CCNGG 1 cut(s) 48
TaaI ACNGT 2 cut(s) 59, 320
TaqI TCGA 5 cut(s) 17, 39, 117, 288, 510
TasI AATT 2 cut(s) 170, 494
TauI GCSGC 1 cut(s) 129
TfiI GAWTC 3 cut(s) 311, 428, 446
Tru1I TTAA 3 cut(s) 228, 300, 455
Tru9I TTAA 3 cut(s) 228, 300, 455
TscAI CASTG 1 cut(s) 62
TseI GCWGC 2 cut(s) 123, 277
TspDTI ATGAA 2 cut(s) 370, 438
TspGWI ACGGA 1 cut(s) 151
TspRI CASTG 1 cut(s) 62
XapI RAATTY 1 cut(s) 170
XceI RCATGY 2 cut(s) 349, 419
XspI CTAG 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.