FvH4_6g33150

Calcineurin B-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002018
Physical Location & Seq
Forward (+)
26209301 .. 26211808
2508 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g33150.t1

Sequence Viewer

Length: 963 bp
ATGTCTCTTAAACCGGACCATGTACTGGTCTTACTCTTCCGGTTGTCTATCTCTTCCGGTTCTGTCTCCGGAGTAAAATCCAATCCATTTGAGTTGGATTATAGATTTATTGATTGCTTCGATTCTCTAATGACGACGCCGACGATCACCACCGAGAGGCAGCGAGCTCTTCTCCTCCGCGATCTTCTTTTGCGTCGCGACATGAACGCCCGGCTCAGCTTTCTGAATTCGAGCTCGAGCTCGCTCCATATCGGCGAGAAGCTCTGCGCCATGTGCATTCCGGTGGTTGGTCTTATTGAGGCTTTCATCTTCAGCTTAGCTGGTTGCTTCCAACACCATCGTCCACGTCATCCGCAGCCAGCCGCTCTCACCTACGACGATCTCGTTCGCCTCGCTGCCACCTCCCCATTTAGTGTGAATGAGGTGGAAGCTTTGCGGGAATTGTTTAACAAGTTGAGTAGTTCGATTCTGGATGATGGTTTAATTCACAAGGAGGAGCTTAGGTTGGCTCTTGTCCAAACTCAAGCAGGCGAAAATCTTTTTCTTGATAGAGTATTTGATGCTTTTGATGAAAAGAAAAATGGTGTCATTGAATTTGAGGAGTTTATCCATGCATTAAGTGTATTTCATCCCTGCACTCCTCTTGAAGAGAAGATTGATTTTGCATTCAGGTTGTATGATTTGAGACAGACTGGATACATTGAGCGGGAAGAAGTTAAGCAAATGGTAGTTGCCACTTTGTTGGAATCCGGCATACAAGTGCCAGATGAAACTGTCGAAGCTATTGTGGACAAGACATTTCAAGATGCTGATGCCGATAGAGATGACAAAATAAGCAACGATGAGTGGAAGGCTTTTGTATTTCAGCACCCCACACTCCTAAAGAATATGACACTTCCATATTTAAAGGACATCACCACAGTCTTCCCAAGTTTTATTTTCAACACTGAAGTCGAGGACTGA

Protein Analysis

321

Amino Acids

36.45

Weight (kDa)

4.91

Isoelectric Point (pI)

40.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 216 - 282 3.9e-09 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 25
AccBSI CCGCTC 2 cut(s) 365, 706
AccII CGCG 2 cut(s) 180, 198
AccIII TCCGGA 1 cut(s) 68
AciI CCGC 5 cut(s) 178, 353, 363, 436, 706
AcsI RAATTY 2 cut(s) 226, 593
AcuI CTGAAG 1 cut(s) 295
AcyI GRCGYC 1 cut(s) 137
AfaI GTAC 1 cut(s) 24
AfiI CCNNNNNNNGG 3 cut(s) 25, 156, 287
AgsI TTSAA 4 cut(s) 593, 647, 803, 943
AjiI CACGTC 1 cut(s) 347
Alw21I GWGCWC 3 cut(s) 169, 236, 242
Alw26I GTCTC 3 cut(s) 9, 70, 679
Ama87I CYCGRG 1 cut(s) 235
Aor13HI TCCGGA 1 cut(s) 68
ApeKI GCWGC 3 cut(s) 160, 355, 395
ApoI RAATTY 2 cut(s) 226, 593
AspLEI GCGC 1 cut(s) 269
AspS9I GGNCC 1 cut(s) 16
AsuC2I CCSGG 1 cut(s) 211
AsuHPI GGTGA 3 cut(s) 139, 361, 907
AvaI CYCGRG 1 cut(s) 235
AvaII GGWCC 1 cut(s) 16
BanII GRGCYC 3 cut(s) 169, 236, 242
BbsI GAAGAC 1 cut(s) 916
Bbv12I GWGCWC 3 cut(s) 169, 236, 242
BbvI GCAGC 3 cut(s) 172, 367, 382
BccI CCATC 2 cut(s) 345, 470
BciVI GTATCC 1 cut(s) 689
BcnI CCSGG 1 cut(s) 211
BcoDI GTCTC 3 cut(s) 9, 70, 679
BfuI GTATCC 1 cut(s) 689
BisI GCNGC 4 cut(s) 161, 356, 363, 396
BlpI GCTNAGC 2 cut(s) 215, 316
BlsI GCNGC 4 cut(s) 162, 357, 364, 397
Bme1390I CCNGG 1 cut(s) 211
Bme18I GGWCC 1 cut(s) 16
BmeT110I CYCGRG 1 cut(s) 235
BmgBI CACGTC 1 cut(s) 347
BmgT120I GGNCC 1 cut(s) 16
BmrFI CCNGG 1 cut(s) 211
BmsI GCATC 3 cut(s) 550, 796, 802
BpiI GAAGAC 1 cut(s) 916
BplI GAGNNNNNCTC 2 cut(s) 156, 188
Bpu10I CCTNAGC 1 cut(s) 500
Bpu1102I GCTNAGC 2 cut(s) 215, 316
BpuEI CTTGAG 1 cut(s) 507
BpuMI CCSGG 1 cut(s) 211
BsaHI GRCGYC 1 cut(s) 137
BsaWI WCCGGW 5 cut(s) 13, 39, 56, 68, 280
BsaXI ACNNNNNCTCC 2 cut(s) 488, 518
Bsc4I CCNNNNNNNGG 3 cut(s) 25, 156, 287
Bse1I ACTGG 2 cut(s) 30, 697
BseAI TCCGGA 1 cut(s) 68
BseGI GGATG 3 cut(s) 349, 478, 628
BseLI CCNNNNNNNGG 3 cut(s) 25, 156, 287
BseMII CTCAG 1 cut(s) 229
BseNI ACTGG 2 cut(s) 30, 697
BseRI GAGGAG 4 cut(s) 164, 509, 614, 630
BseXI GCAGC 3 cut(s) 172, 367, 382
BsgI GTGCAG 1 cut(s) 619
Bsh1236I CGCG 2 cut(s) 180, 198
BsiHKAI GWGCWC 3 cut(s) 169, 236, 242
BsiHKCI CYCGRG 1 cut(s) 235
BsiSI CCGG 7 cut(s) 14, 40, 57, 69, 211, 281, 750
BslI CCNNNNNNNGG 3 cut(s) 25, 156, 287
BsmAI GTCTC 3 cut(s) 9, 70, 679
BsmI GAATGC 2 cut(s) 276, 665
BsoBI CYCGRG 1 cut(s) 235
Bsp1286I GDGCHC 3 cut(s) 169, 236, 242
Bsp13I TCCGGA 1 cut(s) 68
Bsp143I GATC 3 cut(s) 144, 181, 379
Bsp1720I GCTNAGC 2 cut(s) 215, 316
Bsp68I TCGCGA 1 cut(s) 198
BspACI CCGC 5 cut(s) 178, 353, 363, 436, 706
BspCNI CTCAG 1 cut(s) 228
BspEI TCCGGA 1 cut(s) 68
BspFNI CGCG 2 cut(s) 180, 198
BspQI GCTCTTC 1 cut(s) 174
BsrBI CCGCTC 2 cut(s) 365, 706
BsrI ACTGG 2 cut(s) 30, 697
BssMI GATC 3 cut(s) 144, 181, 379
BssNI GRCGYC 1 cut(s) 137
Bst4CI ACNGT 2 cut(s) 775, 922
Bst6I CTCTTC 4 cut(s) 41, 58, 174, 642
BstACI GRCGYC 1 cut(s) 137
BstC8I GCNNGC 4 cut(s) 165, 242, 360, 529
BstDEI CTNAG 3 cut(s) 215, 316, 500
BstF5I GGATG 3 cut(s) 349, 478, 628
BstFNI CGCG 2 cut(s) 180, 198
BstHHI GCGC 1 cut(s) 269
BstKTI GATC 3 cut(s) 147, 184, 382
BstMAI GTCTC 3 cut(s) 9, 70, 679
BstMBI GATC 3 cut(s) 144, 181, 379
BstMWI GCNNNNNNNGC 1 cut(s) 273
BstSCI CCNGG 1 cut(s) 209
BstUI CGCG 2 cut(s) 180, 198
BstV1I GCAGC 3 cut(s) 172, 367, 382
BstV2I GAAGAC 1 cut(s) 916
BstXI CCANNNNNNTGG 1 cut(s) 742
BsuI GTATCC 1 cut(s) 689
BtrI CACGTC 1 cut(s) 347
BtsCI GGATG 3 cut(s) 349, 478, 628
BtsIMutI CAGTG 1 cut(s) 945
BtuMI TCGCGA 1 cut(s) 198
Cac8I GCNNGC 4 cut(s) 165, 242, 360, 529
CfoI GCGC 1 cut(s) 269
Cfr13I GGNCC 1 cut(s) 16
CseI GACGC 2 cut(s) 145, 182
Csp6I GTAC 1 cut(s) 23
CviAII CATG 4 cut(s) 20, 202, 271, 611
CviQI GTAC 1 cut(s) 23
DdeI CTNAG 3 cut(s) 215, 316, 500
DpnI GATC 3 cut(s) 146, 183, 381
DpnII GATC 3 cut(s) 144, 181, 379
DraI TTTAAA 1 cut(s) 906
Eam1104I CTCTTC 4 cut(s) 41, 58, 174, 642
EarI CTCTTC 4 cut(s) 41, 58, 174, 642
Ecl136II GAGCTC 3 cut(s) 167, 234, 240
Eco24I GRGCYC 3 cut(s) 169, 236, 242
Eco47I GGWCC 1 cut(s) 16
Eco53kI GAGCTC 3 cut(s) 167, 234, 240
Eco57I CTGAAG 1 cut(s) 295
Eco88I CYCGRG 1 cut(s) 235
EcoICRI GAGCTC 3 cut(s) 167, 234, 240
EcoRI GAATTC 1 cut(s) 226
EcoT22I ATGCAT 1 cut(s) 616
EcoT38I GRGCYC 3 cut(s) 169, 236, 242
FaeI CATG 4 cut(s) 23, 205, 274, 614
FatI CATG 4 cut(s) 19, 201, 270, 610
FauI CCCGC 2 cut(s) 429, 699
Fnu4HI GCNGC 4 cut(s) 161, 356, 363, 396
FokI GGATG 3 cut(s) 336, 485, 615
FriOI GRGCYC 3 cut(s) 169, 236, 242
Fsp4HI GCNGC 4 cut(s) 161, 356, 363, 396
GlaI GCGC 1 cut(s) 268
GluI GCNGC 4 cut(s) 161, 356, 363, 396
HapII CCGG 7 cut(s) 14, 40, 57, 69, 211, 281, 750
HgaI GACGC 2 cut(s) 145, 182
HhaI GCGC 1 cut(s) 269
Hin1I GRCGYC 1 cut(s) 137
Hin1II CATG 4 cut(s) 23, 205, 274, 614
Hin6I GCGC 1 cut(s) 267
HinP1I GCGC 1 cut(s) 267
HindIII AAGCTT 1 cut(s) 429
HinfI GANTC 3 cut(s) 122, 466, 746
HpaII CCGG 7 cut(s) 14, 40, 57, 69, 211, 281, 750
HphI GGTGA 3 cut(s) 139, 361, 907
Hpy166II GTNNAC 2 cut(s) 344, 790
Hpy188I TCNGA 1 cut(s) 225
Hpy188III TCNNGA 6 cut(s) 69, 197, 470, 545, 644, 803
Hpy8I GTNNAC 2 cut(s) 344, 790
Hpy99I CGWCG 4 cut(s) 139, 145, 198, 380
HpyAV CCTTC 1 cut(s) 844
HpyCH4III ACNGT 2 cut(s) 775, 922
HpyCH4IV ACGT 1 cut(s) 346
HpyCH4V TGCA 4 cut(s) 276, 614, 636, 665
HpyF10VI GCNNNNNNNGC 1 cut(s) 273
HpyF3I CTNAG 3 cut(s) 215, 316, 500
HpySE526I ACGT 1 cut(s) 346
Hsp92I GRCGYC 1 cut(s) 137
Hsp92II CATG 4 cut(s) 23, 205, 274, 614
HspAI GCGC 1 cut(s) 267
Kpn2I TCCGGA 1 cut(s) 68
Kzo9I GATC 3 cut(s) 144, 181, 379
LguI GCTCTTC 1 cut(s) 174
LmnI GCTCC 2 cut(s) 249, 496
Lsp1109I GCAGC 3 cut(s) 172, 367, 382
LweI GCATC 3 cut(s) 550, 796, 802
MaeII ACGT 1 cut(s) 346
MalI GATC 3 cut(s) 146, 183, 381
MbiI CCGCTC 2 cut(s) 365, 706
MboI GATC 3 cut(s) 144, 181, 379
MboII GAAGA 9 cut(s) 28, 45, 161, 176, 301, 659, 664, 722, 916
MhlI GDGCHC 3 cut(s) 169, 236, 242
MluCI AATT 4 cut(s) 226, 440, 483, 593
MmeI TCCRAC 3 cut(s) 75, 355, 723
Mph1103I ATGCAT 1 cut(s) 616
MroI TCCGGA 1 cut(s) 68
MseI TTAA 6 cut(s) 9, 447, 482, 617, 717, 905
MslI CAYNNNNRTG 2 cut(s) 281, 758
MspI CCGG 7 cut(s) 14, 40, 57, 69, 211, 281, 750
MspR9I CCNGG 1 cut(s) 211
Mva1269I GAATGC 2 cut(s) 276, 665
MvnI CGCG 2 cut(s) 180, 198
MwoI GCNNNNNNNGC 1 cut(s) 273
NciI CCSGG 1 cut(s) 211
NdeII GATC 3 cut(s) 144, 181, 379
NlaIII CATG 4 cut(s) 23, 205, 274, 614
NruI TCGCGA 1 cut(s) 198
NsiI ATGCAT 1 cut(s) 616
PaeR7I CTCGAG 1 cut(s) 235
PciSI GCTCTTC 1 cut(s) 174
PcsI WCGNNNNNNNCGW 2 cut(s) 140, 381
PctI GAATGC 2 cut(s) 276, 665
PfeI GAWTC 3 cut(s) 122, 466, 746
PflMI CCANNNNNTGG 1 cut(s) 25
PkrI GCNGC 4 cut(s) 162, 357, 364, 397
Psp124BI GAGCTC 3 cut(s) 169, 236, 242
PspPI GGNCC 1 cut(s) 16
PspXI VCTCGAGB 1 cut(s) 235
RruI TCGCGA 1 cut(s) 198
RsaI GTAC 1 cut(s) 24
RsaNI GTAC 1 cut(s) 23
RseI CAYNNNNRTG 2 cut(s) 281, 758
SacI GAGCTC 3 cut(s) 169, 236, 242
SapI GCTCTTC 1 cut(s) 174
SaqAI TTAA 6 cut(s) 9, 447, 482, 617, 717, 905
SatI GCNGC 4 cut(s) 161, 356, 363, 396
Sau3AI GATC 3 cut(s) 144, 181, 379
Sau96I GGNCC 1 cut(s) 16
ScrFI CCNGG 1 cut(s) 211
SduI GDGCHC 3 cut(s) 169, 236, 242
SfaNI GCATC 3 cut(s) 550, 796, 802
Sfr274I CTCGAG 1 cut(s) 235
SinI GGWCC 1 cut(s) 16
SlaI CTCGAG 1 cut(s) 235
SmiMI CAYNNNNRTG 2 cut(s) 281, 758
SmlI CTYRAG 2 cut(s) 235, 522
SmoI CTYRAG 2 cut(s) 235, 522
Sse9I AATT 4 cut(s) 226, 440, 483, 593
SsiI CCGC 5 cut(s) 178, 353, 363, 436, 706
SstI GAGCTC 3 cut(s) 169, 236, 242
StyD4I CCNGG 1 cut(s) 209
TaaI ACNGT 2 cut(s) 775, 922
TaiI ACGT 1 cut(s) 349
TaqI TCGA 6 cut(s) 120, 230, 236, 464, 777, 954
TasI AATT 4 cut(s) 226, 440, 483, 593
TatI WGTACW 1 cut(s) 22
TauI GCSGC 1 cut(s) 365
TfiI GAWTC 3 cut(s) 122, 466, 746
Tru1I TTAA 6 cut(s) 9, 447, 482, 617, 717, 905
Tru9I TTAA 6 cut(s) 9, 447, 482, 617, 717, 905
TscAI CASTG 1 cut(s) 952
TseI GCWGC 3 cut(s) 160, 355, 395
TspDTI ATGAA 5 cut(s) 218, 295, 585, 617, 783
TspRI CASTG 1 cut(s) 952
Van91I CCANNNNNTGG 1 cut(s) 25
VpaK11BI GGWCC 1 cut(s) 16
XapI RAATTY 2 cut(s) 226, 593
XhoI CTCGAG 1 cut(s) 235
Zsp2I ATGCAT 1 cut(s) 616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.