RchiOBHm_Chr2g0142841

Calcineurin B-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002018
Physical Location & Seq
Forward (+)
60510042 .. 60513242
3201 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51297

Sequence Viewer

Length: 945 bp
ATGACGACGACGAACACAGAGAGGCAGAGAGCTCTTCTTCTCCGCGATGTTCTGTTGCGCAGGGACATGGATGCCAGCCTCACCTTTCTACAGAATTCGAGCACGAGCTCGCTCCGAATCCGCGAGAAGCTGTGTGCGTTGTGCGTACCAGTGGTTGGTCTCATTCAGGCTTTCATCTTCAGCTTAGTTGGGTGCTTCGATCACCACCTTCCACGTCATCAACACCGAGCCGCTTTCACCCACGACGACGTCGTTCGCCTCGCTGCCACCTCCCCATTTGGTGTAAATGAGGTGGAAGCATTGCGTGAATTGTTCAACAAGTTGAGCAGTTCGATTCTAGATGATGGCTTAATTCACAAGGAGGATCTTAGATTGGCTCTGTTCAAAACTCAATCAGGCGAAAATCTTTTTCTTGATAGGGTATTTGATGTTTTTGACGAAAAGAAAGATGGCGTCATTGAATTTGAGGAGTTTATCCATGCATTAAGTGTATTTCATCCCTGCACTCCTATTGAACAGAAGATTGACTTTGCATTCAGGTTGTATGATTTGAGACAGACTGGATATATTGAGTGGGAAGAAGTTAGGAAAATGGTAGTCGCCACTTTGTTGGAATCCGGCATACAGGTGCCGGATGAAACTCTTGAAACAATTGTGGATAAGGCAAGACTCATGAGCGTTCAATCTTTTGTATTTGTTCTATTTTTCTTTATGTGTGCTTGCCAAACTTCTCCTGAAATTTTATACTCTGAAAGAAGGCATGTTTCCATTCTTCAGACATTTGAAGACGCTGATGCCGATAAGGATGGCAAAATTAGCAATGATGAATGGAAGGCTTTTGTATTTCGGCAGCCTACACTCGTAAAGAACATGACACTTCCATATTTAAAGGACATCACCACAGTCTTCCCAAGTTTTATTTTCAACACTGAAGTCGAGGACTGA

Protein Analysis

314

Amino Acids

36.2

Weight (kDa)

5.13

Isoelectric Point (pI)

50.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_5 PF13202 260 - 281 1.2e-06 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 252
Acc16I TGCGCA 1 cut(s) 59
AccB1I GGYRCC 1 cut(s) 628
AccB7I CCANNNNNTGG 1 cut(s) 155
AccII CGCG 2 cut(s) 45, 123
AciI CCGC 3 cut(s) 43, 121, 231
AclWI GGATC 1 cut(s) 372
AcsI RAATTY 3 cut(s) 94, 461, 738
AcuI CTGAAG 2 cut(s) 163, 758
AcyI GRCGYC 2 cut(s) 249, 453
AfaI GTAC 1 cut(s) 147
AfiI CCNNNNNNNGG 1 cut(s) 155
AgsI TTSAA 8 cut(s) 316, 385, 461, 515, 647, 683, 785, 925
AjiI CACGTC 1 cut(s) 215
AluBI AGCT 4 cut(s) 32, 108, 130, 183
AluI AGCT 4 cut(s) 32, 108, 130, 183
Alw21I GWGCWC 3 cut(s) 34, 104, 110
Alw26I GTCTC 2 cut(s) 164, 547
AlwI GGATC 1 cut(s) 372
ApeKI GCWGC 2 cut(s) 263, 850
ApoI RAATTY 3 cut(s) 94, 461, 738
AspLEI GCGC 1 cut(s) 60
AsuHPI GGTGA 4 cut(s) 73, 194, 229, 889
BanI GGYRCC 1 cut(s) 628
BanII GRGCYC 2 cut(s) 34, 110
BauI CACGAG 1 cut(s) 103
BbsI GAAGAC 2 cut(s) 792, 898
Bbv12I GWGCWC 3 cut(s) 34, 104, 110
BbvI GCAGC 2 cut(s) 250, 862
BccI CCATC 3 cut(s) 338, 443, 800
BcoDI GTCTC 2 cut(s) 164, 547
BfaI CTAG 1 cut(s) 338
BfmI CTRYAG 1 cut(s) 89
BisI GCNGC 3 cut(s) 231, 264, 851
BlsI GCNGC 3 cut(s) 232, 265, 852
BmgBI CACGTC 1 cut(s) 215
BmiI GGNNCC 1 cut(s) 630
BmsI GCATC 2 cut(s) 61, 784
BpiI GAAGAC 2 cut(s) 792, 898
BsaHI GRCGYC 2 cut(s) 249, 453
BsaI GGTCTC 1 cut(s) 164
Bsc4I CCNNNNNNNGG 1 cut(s) 155
Bse1I ACTGG 2 cut(s) 149, 565
Bse3DI GCAATG 2 cut(s) 299, 826
BseGI GGATG 4 cut(s) 76, 496, 640, 811
BseLI CCNNNNNNNGG 1 cut(s) 155
BseMI GCAATG 2 cut(s) 299, 826
BseNI ACTGG 2 cut(s) 149, 565
BseRI GAGGAG 1 cut(s) 482
BseXI GCAGC 2 cut(s) 250, 862
BsgI GTGCAG 1 cut(s) 487
Bsh1236I CGCG 2 cut(s) 45, 123
BshNI GGYRCC 1 cut(s) 628
BsiHKAI GWGCWC 3 cut(s) 34, 104, 110
BsiSI CCGG 2 cut(s) 618, 632
BslFI GGGAC 1 cut(s) 77
BslI CCNNNNNNNGG 1 cut(s) 155
BsmAI GTCTC 2 cut(s) 164, 547
BsmFI GGGAC 1 cut(s) 77
BsmI GAATGC 1 cut(s) 533
Bso31I GGTCTC 1 cut(s) 164
Bsp1286I GDGCHC 3 cut(s) 34, 104, 110
Bsp143I GATC 2 cut(s) 199, 364
BspACI CCGC 3 cut(s) 43, 121, 231
BspFNI CGCG 2 cut(s) 45, 123
BspHI TCATGA 1 cut(s) 672
BspLI GGNNCC 1 cut(s) 630
BspPI GGATC 1 cut(s) 372
BspQI GCTCTTC 1 cut(s) 39
BspT107I GGYRCC 1 cut(s) 628
BspTNI GGTCTC 1 cut(s) 164
BsrDI GCAATG 2 cut(s) 299, 826
BsrI ACTGG 2 cut(s) 149, 565
BssMI GATC 2 cut(s) 199, 364
BssNI GRCGYC 2 cut(s) 249, 453
BssSI CACGAG 1 cut(s) 103
Bst2BI CACGAG 1 cut(s) 103
Bst4CI ACNGT 1 cut(s) 904
Bst6I CTCTTC 1 cut(s) 39
BstACI GRCGYC 2 cut(s) 249, 453
BstC8I GCNNGC 3 cut(s) 76, 110, 721
BstDEI CTNAG 2 cut(s) 184, 368
BstF5I GGATG 4 cut(s) 76, 496, 640, 811
BstFNI CGCG 2 cut(s) 45, 123
BstHHI GCGC 1 cut(s) 60
BstKTI GATC 2 cut(s) 202, 367
BstMAI GTCTC 2 cut(s) 164, 547
BstMBI GATC 2 cut(s) 199, 364
BstMWI GCNNNNNNNGC 1 cut(s) 816
BstNSI RCATGY 1 cut(s) 764
BstSFI CTRYAG 1 cut(s) 89
BstUI CGCG 2 cut(s) 45, 123
BstV1I GCAGC 2 cut(s) 250, 862
BstV2I GAAGAC 2 cut(s) 792, 898
BstX2I RGATCY 1 cut(s) 364
BstXI CCANNNNNNTGG 1 cut(s) 610
BstYI RGATCY 1 cut(s) 364
BtgZI GCGATG 1 cut(s) 60
BtrI CACGTC 1 cut(s) 215
BtsCI GGATG 4 cut(s) 76, 496, 640, 811
BtsIMutI CAGTG 2 cut(s) 156, 927
Cac8I GCNNGC 3 cut(s) 76, 110, 721
CciI TCATGA 1 cut(s) 672
CfoI GCGC 1 cut(s) 60
CseI GACGC 2 cut(s) 442, 797
Csp6I GTAC 1 cut(s) 146
CviAII CATG 5 cut(s) 67, 479, 673, 761, 871
CviQI GTAC 1 cut(s) 146
DdeI CTNAG 2 cut(s) 184, 368
DpnI GATC 2 cut(s) 201, 366
DpnII GATC 2 cut(s) 199, 364
DraI TTTAAA 1 cut(s) 888
Eam1104I CTCTTC 1 cut(s) 39
EarI CTCTTC 1 cut(s) 39
Ecl136II GAGCTC 2 cut(s) 32, 108
Eco24I GRGCYC 2 cut(s) 34, 110
Eco31I GGTCTC 1 cut(s) 164
Eco53kI GAGCTC 2 cut(s) 32, 108
Eco57I CTGAAG 2 cut(s) 163, 758
EcoICRI GAGCTC 2 cut(s) 32, 108
EcoRI GAATTC 1 cut(s) 94
EcoT22I ATGCAT 1 cut(s) 484
EcoT38I GRGCYC 2 cut(s) 34, 110
FaeI CATG 5 cut(s) 70, 482, 676, 764, 874
FalI AAGNNNNNCTT 2 cut(s) 512, 544
FaqI GGGAC 1 cut(s) 77
FatI CATG 5 cut(s) 66, 478, 672, 760, 870
Fnu4HI GCNGC 3 cut(s) 231, 264, 851
FokI GGATG 4 cut(s) 83, 483, 647, 818
FriOI GRGCYC 2 cut(s) 34, 110
Fsp4HI GCNGC 3 cut(s) 231, 264, 851
FspBI CTAG 1 cut(s) 338
FspI TGCGCA 1 cut(s) 59
GlaI GCGC 1 cut(s) 59
GluI GCNGC 3 cut(s) 231, 264, 851
HapII CCGG 2 cut(s) 618, 632
HgaI GACGC 2 cut(s) 442, 797
HhaI GCGC 1 cut(s) 60
Hin1I GRCGYC 2 cut(s) 249, 453
Hin1II CATG 5 cut(s) 70, 482, 676, 764, 874
Hin6I GCGC 1 cut(s) 58
HinP1I GCGC 1 cut(s) 58
HinfI GANTC 4 cut(s) 117, 334, 614, 669
HpaII CCGG 2 cut(s) 618, 632
HphI GGTGA 4 cut(s) 73, 194, 229, 889
Hpy188I TCNGA 3 cut(s) 116, 751, 777
Hpy188III TCNNGA 5 cut(s) 338, 413, 644, 673, 734
Hpy99I CGWCG 5 cut(s) 10, 13, 248, 251, 254
HpyAV CCTTC 3 cut(s) 218, 750, 826
HpyCH4III ACNGT 1 cut(s) 904
HpyCH4IV ACGT 2 cut(s) 214, 249
HpyCH4V TGCA 3 cut(s) 482, 504, 533
HpyF10VI GCNNNNNNNGC 1 cut(s) 816
HpyF3I CTNAG 2 cut(s) 184, 368
HpySE526I ACGT 2 cut(s) 214, 249
Hsp92I GRCGYC 2 cut(s) 249, 453
Hsp92II CATG 5 cut(s) 70, 482, 676, 764, 874
HspAI GCGC 1 cut(s) 58
Kzo9I GATC 2 cut(s) 199, 364
LguI GCTCTTC 1 cut(s) 39
LmnI GCTCC 1 cut(s) 117
Lsp1109I GCAGC 2 cut(s) 250, 862
LweI GCATC 2 cut(s) 61, 784
MaeI CTAG 1 cut(s) 338
MaeII ACGT 2 cut(s) 214, 249
MalI GATC 2 cut(s) 201, 366
MboI GATC 2 cut(s) 199, 364
MboII GAAGA 8 cut(s) 26, 29, 169, 532, 590, 764, 797, 898
MfeI CAATTG 1 cut(s) 651
MflI RGATCY 1 cut(s) 364
MhlI GDGCHC 3 cut(s) 34, 104, 110
MluCI AATT 7 cut(s) 94, 308, 351, 461, 651, 738, 813
MlyI GAGTC 1 cut(s) 663
MmeI TCCRAC 1 cut(s) 591
MnlI CCTC 8 cut(s) 15, 89, 269, 280, 283, 355, 460, 931
Mph1103I ATGCAT 1 cut(s) 484
MseI TTAA 3 cut(s) 350, 485, 887
MslI CAYNNNNRTG 1 cut(s) 626
MspI CCGG 2 cut(s) 618, 632
MunI CAATTG 1 cut(s) 651
Mva1269I GAATGC 1 cut(s) 533
MvnI CGCG 2 cut(s) 45, 123
MwoI GCNNNNNNNGC 1 cut(s) 816
NdeII GATC 2 cut(s) 199, 364
NlaIII CATG 5 cut(s) 70, 482, 676, 764, 874
NlaIV GGNNCC 1 cut(s) 630
NsbI TGCGCA 1 cut(s) 59
NsiI ATGCAT 1 cut(s) 484
NspI RCATGY 1 cut(s) 764
PagI TCATGA 1 cut(s) 672
PciSI GCTCTTC 1 cut(s) 39
PcsI WCGNNNNNNNCGW 2 cut(s) 249, 795
PctI GAATGC 1 cut(s) 533
PfeI GAWTC 3 cut(s) 117, 334, 614
PflFI GACNNNGTC 1 cut(s) 248
PflMI CCANNNNNTGG 1 cut(s) 155
PkrI GCNGC 3 cut(s) 232, 265, 852
PleI GAGTC 1 cut(s) 663
PpsI GAGTC 1 cut(s) 663
Psp124BI GAGCTC 2 cut(s) 34, 110
PspN4I GGNNCC 1 cut(s) 630
PsuI RGATCY 1 cut(s) 364
PsyI GACNNNGTC 1 cut(s) 248
RsaI GTAC 1 cut(s) 147
RsaNI GTAC 1 cut(s) 146
RseI CAYNNNNRTG 1 cut(s) 626
SacI GAGCTC 2 cut(s) 34, 110
SapI GCTCTTC 1 cut(s) 39
SaqAI TTAA 3 cut(s) 350, 485, 887
SatI GCNGC 3 cut(s) 231, 264, 851
Sau3AI GATC 2 cut(s) 199, 364
SchI GAGTC 1 cut(s) 663
SduI GDGCHC 3 cut(s) 34, 104, 110
SfaNI GCATC 2 cut(s) 61, 784
SfcI CTRYAG 1 cut(s) 89
SmiMI CAYNNNNRTG 1 cut(s) 626
Sse9I AATT 7 cut(s) 94, 308, 351, 461, 651, 738, 813
SsiI CCGC 3 cut(s) 43, 121, 231
SspMI CTAG 1 cut(s) 338
SstI GAGCTC 2 cut(s) 34, 110
TaaI ACNGT 1 cut(s) 904
TaiI ACGT 2 cut(s) 217, 252
TaqI TCGA 4 cut(s) 98, 198, 332, 936
TasI AATT 7 cut(s) 94, 308, 351, 461, 651, 738, 813
TauI GCSGC 1 cut(s) 233
TfiI GAWTC 3 cut(s) 117, 334, 614
Tru1I TTAA 3 cut(s) 350, 485, 887
Tru9I TTAA 3 cut(s) 350, 485, 887
TscAI CASTG 2 cut(s) 156, 934
TseI GCWGC 2 cut(s) 263, 850
TspDTI ATGAA 4 cut(s) 163, 485, 651, 840
TspRI CASTG 2 cut(s) 156, 934
Tth111I GACNNNGTC 1 cut(s) 248
Van91I CCANNNNNTGG 1 cut(s) 155
XapI RAATTY 3 cut(s) 94, 461, 738
XbaI TCTAGA 1 cut(s) 337
XceI RCATGY 1 cut(s) 764
XspI CTAG 1 cut(s) 338
ZraI GACGTC 1 cut(s) 250
Zsp2I ATGCAT 1 cut(s) 484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.