MD09G1194500.v1.1

Calcineurin B-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002018
Physical Location & Seq
Reverse (-)
17485706 .. 17488584
2879 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1194500.v1.1.491

Sequence Viewer

Length: 780 bp
ATGGCTGAGAGACCCAGATTGCAAATCCGTGACCTTCTATTTCGCATAGATTATTCCGCCTACAGCTTTACGATCGGCGAGACGCTCTGTGCGGTGTGCATACCACTGATTGGACTGGTCGAGGCTTTCATCTTCAGCTTAGCTGGTTGCTTCGATCGCCACCGTCAACCTCCCCGCCCTTCCTTCACCCACGACGATGTTGTTCGACTCGCCGCCACCTCTCCATTTAGTGTGAATGAGGTGGAGGCATTGCGCGAACTGTTTAACAAGTTGAGTAGTTCCGTGCTCGACGACGGATTAATTCACAAGGAGGAGCTGAGTTTGGCGCTTTTCAAGACTCCGGCTGGTGAAAATCTTTTTCTGGATAGAGTGTTTGATGTTTTTGACGAAAAGAAAAATGGGGTGATTGAATTTGACGAGTTTGTCCACGCGCTAAGTGTATTTCATCCGTATGCTCCTCTTGAGGCCAAGATTGATTTCGCGTTTAGCTTGTATGATTTGAGACAAACAGGATATATCGAGCGGGAAGAAGTCAGGCAAATGGTAGTTGCTACTTTGTCGGAATCCGGCATTCAGATGCCGGATGAAACTCTGGAAGCTATCGTCGATAAGACGTTTGAGGATGCCGACGCTGACAAGGATGAGAAAATTAGCAAAGACGAATGGAAAGCTTATGCTATTCGGTACCCAGCAGTCCTAAAGAACATGACACTTCCATATTTAAAGGACATCACGACGGTTTTTCCAAGTTTTATTTTCAACACTGGAGTCGAAGATTGA

Protein Analysis

260

Amino Acids

29.53

Weight (kDa)

4.67

Isoelectric Point (pI)

36.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 156 - 223 6.1e-10 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 422
Acc65I GGTACC 1 cut(s) 684
AccB1I GGYRCC 1 cut(s) 684
AccB7I CCANNNNNTGG 1 cut(s) 110
AccBSI CCGCTC 1 cut(s) 523
AccII CGCG 3 cut(s) 255, 431, 482
AciI CCGC 5 cut(s) 57, 92, 175, 213, 523
AcsI RAATTY 1 cut(s) 410
AcuI CTGAAG 1 cut(s) 118
AfaI GTAC 1 cut(s) 686
AfiI CCNNNNNNNGG 1 cut(s) 110
AgsI TTSAA 3 cut(s) 334, 410, 760
AjuI GAANNNNNNNTTGG 2 cut(s) 461, 493
AluBI AGCT 7 cut(s) 66, 138, 143, 316, 489, 599, 671
AluI AGCT 7 cut(s) 66, 138, 143, 316, 489, 599, 671
Alw21I GWGCWC 1 cut(s) 288
Alw26I GTCTC 3 cut(s) 4, 74, 496
AoxI GGCC 1 cut(s) 465
ApoI RAATTY 1 cut(s) 410
AseI ATTAAT 1 cut(s) 299
Asp718I GGTACC 1 cut(s) 684
AspLEI GCGC 3 cut(s) 255, 328, 433
AsuHPI GGTGA 3 cut(s) 178, 359, 415
BanI GGYRCC 1 cut(s) 684
Bbv12I GWGCWC 1 cut(s) 288
BcoDI GTCTC 3 cut(s) 4, 74, 496
BfmI CTRYAG 1 cut(s) 61
BfoI RGCGCY 1 cut(s) 329
BisI GCNGC 1 cut(s) 213
BlpI GCTNAGC 1 cut(s) 139
BlsI GCNGC 1 cut(s) 214
BmiI GGNNCC 1 cut(s) 686
BmsI GCATC 2 cut(s) 567, 613
Bpu1102I GCTNAGC 1 cut(s) 139
BpuEI CTTGAG 1 cut(s) 482
BsaI GGTCTC 1 cut(s) 4
Bsc4I CCNNNNNNNGG 1 cut(s) 110
Bse1I ACTGG 2 cut(s) 120, 769
Bse3DI GCAATG 1 cut(s) 248
BseGI GGATG 4 cut(s) 445, 589, 628, 646
BseLI CCNNNNNNNGG 1 cut(s) 110
BseMI GCAATG 1 cut(s) 248
BseMII CTCAG 1 cut(s) 308
BseNI ACTGG 2 cut(s) 120, 769
BseRI GAGGAG 2 cut(s) 326, 447
BseYI CCCAGC 1 cut(s) 688
Bsh1236I CGCG 3 cut(s) 255, 431, 482
Bsh1285I CGRYCG 2 cut(s) 75, 157
BshFI GGCC 1 cut(s) 467
BshNI GGYRCC 1 cut(s) 684
BsiEI CGRYCG 2 cut(s) 75, 157
BsiHKAI GWGCWC 1 cut(s) 288
BsiSI CCGG 3 cut(s) 341, 567, 581
BslI CCNNNNNNNGG 1 cut(s) 110
BsmAI GTCTC 3 cut(s) 4, 74, 496
BsmBI CGTCTC 1 cut(s) 74
BsmI GAATGC 1 cut(s) 570
BsnI GGCC 1 cut(s) 467
Bso31I GGTCTC 1 cut(s) 4
Bsp1286I GDGCHC 1 cut(s) 288
Bsp143I GATC 2 cut(s) 72, 154
Bsp1720I GCTNAGC 1 cut(s) 139
BspACI CCGC 5 cut(s) 57, 92, 175, 213, 523
BspANI GGCC 1 cut(s) 467
BspCNI CTCAG 1 cut(s) 309
BspFNI CGCG 3 cut(s) 255, 431, 482
BspLI GGNNCC 1 cut(s) 686
BspT107I GGYRCC 1 cut(s) 684
BspTNI GGTCTC 1 cut(s) 4
BsrBI CCGCTC 1 cut(s) 523
BsrDI GCAATG 1 cut(s) 248
BsrI ACTGG 2 cut(s) 120, 769
BssMI GATC 2 cut(s) 72, 154
Bst4CI ACNGT 3 cut(s) 164, 261, 739
BstDEI CTNAG 4 cut(s) 6, 139, 317, 434
BstF5I GGATG 4 cut(s) 445, 589, 628, 646
BstFNI CGCG 3 cut(s) 255, 431, 482
BstH2I RGCGCY 1 cut(s) 329
BstHHI GCGC 3 cut(s) 255, 328, 433
BstKTI GATC 2 cut(s) 75, 157
BstMAI GTCTC 3 cut(s) 4, 74, 496
BstMBI GATC 2 cut(s) 72, 154
BstMCI CGRYCG 2 cut(s) 75, 157
BstMWI GCNNNNNNNGC 1 cut(s) 156
BstSFI CTRYAG 1 cut(s) 61
BstUI CGCG 3 cut(s) 255, 431, 482
BsuRI GGCC 1 cut(s) 467
BtsCI GGATG 4 cut(s) 445, 589, 628, 646
BtsIMutI CAGTG 2 cut(s) 104, 762
CfoI GCGC 3 cut(s) 255, 328, 433
CseI GACGC 2 cut(s) 91, 638
Csp6I GTAC 1 cut(s) 685
CviAII CATG 1 cut(s) 706
CviQI GTAC 1 cut(s) 685
DdeI CTNAG 4 cut(s) 6, 139, 317, 434
DpnI GATC 2 cut(s) 74, 156
DpnII GATC 2 cut(s) 72, 154
DraI TTTAAA 1 cut(s) 723
DrdI GACNNNNNNGTC 1 cut(s) 422
DseDI GACNNNNNNGTC 1 cut(s) 422
EciI GGCGGA 1 cut(s) 46
Eco31I GGTCTC 1 cut(s) 4
Eco57I CTGAAG 1 cut(s) 118
Esp3I CGTCTC 1 cut(s) 74
FaeI CATG 1 cut(s) 709
FaiI YATR 8 cut(s) 47, 101, 453, 495, 516, 675, 707, 718
FatI CATG 1 cut(s) 705
FauI CCCGC 2 cut(s) 182, 516
Fnu4HI GCNGC 1 cut(s) 213
FokI GGATG 4 cut(s) 432, 596, 635, 653
Fsp4HI GCNGC 1 cut(s) 213
GlaI GCGC 3 cut(s) 254, 327, 432
GluI GCNGC 1 cut(s) 213
GsaI CCCAGC 1 cut(s) 692
HaeII RGCGCY 1 cut(s) 329
HaeIII GGCC 1 cut(s) 467
HapII CCGG 3 cut(s) 341, 567, 581
HgaI GACGC 2 cut(s) 91, 638
HhaI GCGC 3 cut(s) 255, 328, 433
Hin1II CATG 1 cut(s) 709
Hin6I GCGC 3 cut(s) 253, 326, 431
HinP1I GCGC 3 cut(s) 253, 326, 431
HincII GTYRAC 1 cut(s) 167
HindII GTYRAC 1 cut(s) 167
HindIII AAGCTT 1 cut(s) 669
HinfI GANTC 4 cut(s) 207, 337, 563, 768
HpaII CCGG 3 cut(s) 341, 567, 581
HphI GGTGA 3 cut(s) 178, 359, 415
Hpy166II GTNNAC 2 cut(s) 167, 427
Hpy188I TCNGA 2 cut(s) 562, 576
Hpy188III TCNNGA 5 cut(s) 334, 362, 461, 593, 733
Hpy8I GTNNAC 2 cut(s) 167, 427
Hpy99I CGWCG 6 cut(s) 197, 293, 296, 608, 632, 739
HpyAV CCTTC 3 cut(s) 44, 189, 193
HpyCH4III ACNGT 3 cut(s) 164, 261, 739
HpyCH4IV ACGT 1 cut(s) 614
HpyCH4V TGCA 2 cut(s) 22, 99
HpyF10VI GCNNNNNNNGC 1 cut(s) 156
HpyF3I CTNAG 4 cut(s) 6, 139, 317, 434
HpySE526I ACGT 1 cut(s) 614
Hsp92II CATG 1 cut(s) 709
HspAI GCGC 3 cut(s) 253, 326, 431
KpnI GGTACC 1 cut(s) 688
Kzo9I GATC 2 cut(s) 72, 154
LmnI GCTCC 2 cut(s) 313, 460
LweI GCATC 2 cut(s) 567, 613
MaeII ACGT 1 cut(s) 614
MaeIII GTNAC 1 cut(s) 29
MalI GATC 2 cut(s) 74, 156
MbiI CCGCTC 1 cut(s) 523
MboI GATC 2 cut(s) 72, 154
MboII GAAGA 2 cut(s) 124, 539
MhlI GDGCHC 1 cut(s) 288
MluCI AATT 3 cut(s) 300, 410, 648
MlyI GAGTC 3 cut(s) 201, 331, 777
MmeI TCCRAC 1 cut(s) 540
MnlI CCTC 9 cut(s) 115, 180, 229, 232, 238, 304, 457, 468, 613
MseI TTAA 3 cut(s) 264, 299, 722
MslI CAYNNNNRTG 3 cut(s) 195, 450, 575
MspI CCGG 3 cut(s) 341, 567, 581
Mva1269I GAATGC 1 cut(s) 570
MvnI CGCG 3 cut(s) 255, 431, 482
MwoI GCNNNNNNNGC 1 cut(s) 156
NdeII GATC 2 cut(s) 72, 154
NlaIII CATG 1 cut(s) 709
NlaIV GGNNCC 1 cut(s) 686
NmuCI GTSAC 1 cut(s) 29
PctI GAATGC 1 cut(s) 570
PfeI GAWTC 1 cut(s) 563
PflMI CCANNNNNTGG 1 cut(s) 110
PkrI GCNGC 1 cut(s) 214
Ple19I CGATCG 2 cut(s) 75, 157
PleI GAGTC 3 cut(s) 201, 331, 776
PpsI GAGTC 3 cut(s) 201, 331, 776
PshBI ATTAAT 1 cut(s) 299
PspFI CCCAGC 1 cut(s) 688
PspN4I GGNNCC 1 cut(s) 686
PvuI CGATCG 2 cut(s) 75, 157
RsaI GTAC 1 cut(s) 686
RsaNI GTAC 1 cut(s) 685
RseI CAYNNNNRTG 3 cut(s) 195, 450, 575
SaqAI TTAA 3 cut(s) 264, 299, 722
SatI GCNGC 1 cut(s) 213
Sau3AI GATC 2 cut(s) 72, 154
SchI GAGTC 3 cut(s) 201, 331, 777
SduI GDGCHC 1 cut(s) 288
SfaNI GCATC 2 cut(s) 567, 613
SfcI CTRYAG 1 cut(s) 61
SmiMI CAYNNNNRTG 3 cut(s) 195, 450, 575
SmlI CTYRAG 1 cut(s) 461
SmoI CTYRAG 1 cut(s) 461
Sse9I AATT 3 cut(s) 300, 410, 648
SsiI CCGC 5 cut(s) 57, 92, 175, 213, 523
TaaI ACNGT 3 cut(s) 164, 261, 739
TaiI ACGT 1 cut(s) 617
TaqI TCGA 7 cut(s) 120, 153, 205, 288, 519, 606, 771
TasI AATT 3 cut(s) 300, 410, 648
TauI GCSGC 1 cut(s) 215
TfiI GAWTC 1 cut(s) 563
Tru1I TTAA 3 cut(s) 264, 299, 722
Tru9I TTAA 3 cut(s) 264, 299, 722
TscAI CASTG 2 cut(s) 111, 769
TseFI GTSAC 1 cut(s) 29
Tsp45I GTSAC 1 cut(s) 29
TspDTI ATGAA 3 cut(s) 118, 434, 600
TspGWI ACGGA 4 cut(s) 17, 271, 309, 438
TspRI CASTG 2 cut(s) 111, 769
Van91I CCANNNNNTGG 1 cut(s) 110
VspI ATTAAT 1 cut(s) 299
XapI RAATTY 1 cut(s) 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.