Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0002018

Physical Location & Seq

Genomic Coordinates Reverse (-)

3254626 .. 3256803

Gene Structure 2178 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1043100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 741 bp

ATGGAATTCTCGAGATCGAGTTCGTTATCAATCGGAGAGAAGATCTGCGCGGCGTTTATACCGTTTGTAGCTATGGTAGAGGCGCTGGTTTTCGCCGTGAGCGGTTGCTGCAGCCACCGTTCCTGCCGCCCAATCAACCGCGGTTTCGCTTGCGGCTATATGTCTCACCTCGCCGATGAAACCAGATTTACTGTTAATGAGCTGGAGGCGTTGTATGAGCTTTTTAAGAAGCTGAGCAGCTCGATTATCGACGACGGATCGATTCACAAGGAGGAGCTTCAATTAGCACTATTTCAAACTCCCCAAGGCGAAAATCTGTTCCTTGACCGGGTTTTTGATCTATTTGATGAAAAGAAAAATGGTGTCATTGAATTTGATGAGTTTGTTCATGCACTCAATATCTTCCATCCTTACGCCCCTATTGATGACAAAATCGATTTTGCATTTCGGCTGTATGATCTGAGACAAACCGGATTCATTGAGCGAGAAGAGGTTAAGCAAATGGTGATTGCCATATTGATGGAATCCGACGTGAAATTGCCGGATGATATTCTTGAGGAAATCATTGAGAAGACATTTGCCGACGCTGATGCTGACAAAGATGGGAAAATTAACAAAGAAGAATGGAAGGTTTTCGTGCTTCGGCATCCATCGCTTCTAAAAAACATGACTCTTCCTTATTTGAAGGACATCACGACAGTATTTCCGAGTTATATTTTTCACACCGAAGTTGAAGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005886 GO:0006950 GO:0006970 GO:0006972 GO:0008150 GO:0009628 GO:0009651 GO:0009705 GO:0010959 GO:0012505 GO:0016020 GO:0031090 GO:0031410 GO:0031982 GO:0032879 GO:0042538 GO:0042592 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043266 GO:0043269 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0048878 GO:0050789 GO:0050801 GO:0050896 GO:0051049 GO:0065007 GO:0065008 GO:0071944 GO:0097708 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko04010 ko04020 ko04022 ko04114 ko04218 ko04310 ko04360 ko04370 ko04380 ko04650 ko04658 ko04659 ko04660 ko04662 ko04720 ko04724 ko04921 ko04922 ko04924 ko05010 ko05014 ko05031 ko05152 ko05166 ko05167 map04010 map04020 map04022 map04114 map04218 map04310 map04360 map04370 map04380 map04650 map04658 map04659 map04660 map04662 map04720 map04724 map04921 map04922 map04924 map05010 map05014 map05031 map05152 map05166 map05167

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

247

Amino Acids

28.28

Weight (kDa)

4.77

Isoelectric Point (pI)

49.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_7	PF13499	143 - 208	1.8e-10	EF-hand domain pair
EF-hand_1	PF00036	189 - 213	9.3e-06	EF hand domain
EF-hand_5	PF13202	189 - 213	5.1e-07	EF hand

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002018)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33000 AT4G33000 AT4G33000 AT4G33000 AT4G33000 AT4G33000
fragaria_vesca	FvH4_2g32000 FvH4_2g32000 FvH4_2g32000 FvH4_6g33150
malus_domestica	MD08G1043100.v1.1 MD09G1194500.v1.1 MD09G1194600.v1.1
prunus_persica	Prupe.1G412900_v2.0.a1 Prupe.1G412900_v2.0.a1 Prupe.3G051100_v2.0.a1 Prupe.3G051100_v2.0.a1 Prupe.3G051100_v2.0.a1 Prupe.3G051100_v2.0.a1 Prupe.3G051100_v2.0.a1
pyrus_communis	pycom08g03460 pycom09g11150
rosa_chinensis	RchiOBHm_Chr2g0142841 RchiOBHm_Chr2g0142851 RchiOBHm_Chr6g0310771
rosa_laevigata	RLG00000019991 RLG00000019993
rosa_multiflora	Rmu_sc0004688.1_g000005 Rmu_sc0012306.1_g000001 Rmu_sc0013116.1_g000001 Rmu_sc0017450.1_g000001 Rmu_ssc0000395.1_g000015
rosa_roxburghii	Rroxscaffold_2G00102260 Rroxscaffold_2G00103310
rosa_rugosa	Rorug02G0376000.1 Rorug06G0383100
rosa_samantha	Rh2AG428900 Rh2BG438400 Rh2CG414400 Rh6AG496900 Rh6CG512400 Rh6DG499000
rosa_wichuraiana	Rw2G035070 Rw2G035080 Rw6G043270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	102
AccII	CGCG	2 cut(s)	50, 141
AciI	CCGC	6 cut(s)	50, 102, 127, 139, 141, 153
AclWI	GGATC	1 cut(s)	265
AcsI	RAATTY	2 cut(s)	5, 371
AfiI	CCNNNNNNNGG	1 cut(s)	328
AgsI	TTSAA	5 cut(s)	281, 296, 371, 685, 734
AjiI	CACGTC	1 cut(s)	532
AluBI	AGCT	6 cut(s)	71, 202, 220, 232, 240, 277
AluI	AGCT	6 cut(s)	71, 202, 220, 232, 240, 277
Alw26I	GTCTC	2 cut(s)	168, 457
AlwI	GGATC	1 cut(s)	265
Ama87I	CYCGRG	1 cut(s)	10
ApeKI	GCWGC	3 cut(s)	108, 111, 237
ApoI	RAATTY	2 cut(s)	5, 371
ArsI	GACNNNNNNTTYG	2 cut(s)	317, 349
Asp700I	GAANNNNTTC	1 cut(s)	632
AspLEI	GCGC	2 cut(s)	50, 85
AsuC2I	CCSGG	1 cut(s)	329
AsuHPI	GGTGA	2 cut(s)	158, 517
AvaI	CYCGRG	1 cut(s)	10
BbsI	GAAGAC	1 cut(s)	578
BbvI	GCAGC	3 cut(s)	95, 123, 249
BccI	CCATC	4 cut(s)	414, 514, 596, 658
BceAI	ACGGC	1 cut(s)	80
BcgI	CGANNNNNNTGC	2 cut(s)	572, 606
BcnI	CCSGG	1 cut(s)	329
BcoDI	GTCTC	2 cut(s)	168, 457
BfmI	CTRYAG	1 cut(s)	109
BfoI	RGCGCY	1 cut(s)	86
BglII	AGATCT	1 cut(s)	42
BisI	GCNGC	6 cut(s)	51, 109, 112, 127, 154, 238
BlpI	GCTNAGC	1 cut(s)	233
BlsI	GCNGC	6 cut(s)	52, 110, 113, 128, 155, 239
Bme1390I	CCNGG	1 cut(s)	329
BmeT110I	CYCGRG	1 cut(s)	10
BmgBI	CACGTC	1 cut(s)	532
BmrFI	CCNGG	1 cut(s)	329
BmsI	GCATC	2 cut(s)	580, 655
BpiI	GAAGAC	1 cut(s)	578
BpmI	CTGGAG	1 cut(s)	224
Bpu1102I	GCTNAGC	1 cut(s)	233
BpuEI	CTTGAG	1 cut(s)	575
BpuMI	CCSGG	1 cut(s)	329
Bsa29I	ATCGAT	2 cut(s)	260, 435
BsaJI	CCNNGG	2 cut(s)	139, 304
BsaWI	WCCGGW	1 cut(s)	470
BsaXI	ACNNNNNCTCC	2 cut(s)	197, 227
Bsc4I	CCNNNNNNNGG	1 cut(s)	328
BseCI	ATCGAT	2 cut(s)	260, 435
BseDI	CCNNGG	2 cut(s)	139, 304
BseGI	GGATG	3 cut(s)	406, 550, 646
BseLI	CCNNNNNNNGG	1 cut(s)	328
BseMII	CTCAG	2 cut(s)	224, 452
BseRI	GAGGAG	1 cut(s)	287
BseXI	GCAGC	3 cut(s)	95, 123, 249
Bsh1236I	CGCG	2 cut(s)	50, 141
BshVI	ATCGAT	2 cut(s)	260, 435
BsiHKCI	CYCGRG	1 cut(s)	10
BsiSI	CCGG	3 cut(s)	328, 471, 542
BslI	CCNNNNNNNGG	1 cut(s)	328
BsmAI	GTCTC	2 cut(s)	168, 457
BsoBI	CYCGRG	1 cut(s)	10
Bsp143I	GATC	5 cut(s)	14, 42, 257, 337, 457
Bsp1720I	GCTNAGC	1 cut(s)	233
BspACI	CCGC	6 cut(s)	50, 102, 127, 139, 141, 153
BspCNI	CTCAG	2 cut(s)	225, 453
BspDI	ATCGAT	2 cut(s)	260, 435
BspFNI	CGCG	2 cut(s)	50, 141
BspMAI	CTGCAG	1 cut(s)	113
BspPI	GGATC	1 cut(s)	265
BsrBI	CCGCTC	1 cut(s)	102
BssECI	CCNNGG	2 cut(s)	139, 304
BssMI	GATC	5 cut(s)	14, 42, 257, 337, 457
BssT1I	CCWWGG	1 cut(s)	304
Bst4CI	ACNGT	4 cut(s)	63, 119, 193, 700
Bst6I	CTCTTC	2 cut(s)	483, 678
BstC8I	GCNNGC	1 cut(s)	151
BstDEI	CTNAG	2 cut(s)	233, 461
BstDSI	CCRYGG	1 cut(s)	139
BstF5I	GGATG	3 cut(s)	406, 550, 646
BstFNI	CGCG	2 cut(s)	50, 141
BstH2I	RGCGCY	1 cut(s)	86
BstHHI	GCGC	2 cut(s)	50, 85
BstKTI	GATC	5 cut(s)	17, 45, 260, 340, 460
BstMAI	GTCTC	2 cut(s)	168, 457
BstMBI	GATC	5 cut(s)	14, 42, 257, 337, 457
BstMWI	GCNNNNNNNGC	2 cut(s)	108, 652
BstSCI	CCNGG	1 cut(s)	327
BstSFI	CTRYAG	1 cut(s)	109
BstUI	CGCG	2 cut(s)	50, 141
BstV1I	GCAGC	3 cut(s)	95, 123, 249
BstV2I	GAAGAC	1 cut(s)	578
BstX2I	RGATCY	1 cut(s)	42
BstXI	CCANNNNNNTGG	1 cut(s)	520
BstYI	RGATCY	1 cut(s)	42
Bsu15I	ATCGAT	2 cut(s)	260, 435
BsuTUI	ATCGAT	2 cut(s)	260, 435
BtgI	CCRYGG	1 cut(s)	139
BtgZI	GCGATG	1 cut(s)	636
BtrI	CACGTC	1 cut(s)	532
BtsCI	GGATG	3 cut(s)	406, 550, 646
Cac8I	GCNNGC	1 cut(s)	151
CfoI	GCGC	2 cut(s)	50, 85
Cfr42I	CCGCGG	1 cut(s)	142
ClaI	ATCGAT	2 cut(s)	260, 435
CseI	GACGC	1 cut(s)	593
CviAII	CATG	2 cut(s)	389, 667
CviJI	RGCY	9 cut(s)	71, 114, 156, 202, 220, 232, 240, 277, 451
CviKI_1	RGCY	9 cut(s)	71, 114, 156, 202, 220, 232, 240, 277, 451
DdeI	CTNAG	2 cut(s)	233, 461
DpnI	GATC	5 cut(s)	16, 44, 259, 339, 459
DpnII	GATC	5 cut(s)	14, 42, 257, 337, 457
Eam1104I	CTCTTC	2 cut(s)	483, 678
EarI	CTCTTC	2 cut(s)	483, 678
Eco130I	CCWWGG	1 cut(s)	304
Eco88I	CYCGRG	1 cut(s)	10
EcoRI	GAATTC	1 cut(s)	5
EcoT14I	CCWWGG	1 cut(s)	304
ErhI	CCWWGG	1 cut(s)	304
FaeI	CATG	2 cut(s)	392, 670
FatI	CATG	2 cut(s)	388, 666
Fnu4HI	GCNGC	6 cut(s)	51, 109, 112, 127, 154, 238
FokI	GGATG	3 cut(s)	393, 557, 633
Fsp4HI	GCNGC	6 cut(s)	51, 109, 112, 127, 154, 238
GlaI	GCGC	2 cut(s)	49, 84
GluI	GCNGC	6 cut(s)	51, 109, 112, 127, 154, 238
GsuI	CTGGAG	1 cut(s)	224
HaeII	RGCGCY	1 cut(s)	86
HapII	CCGG	3 cut(s)	328, 471, 542
HgaI	GACGC	1 cut(s)	593
HhaI	GCGC	2 cut(s)	50, 85
Hin1II	CATG	2 cut(s)	392, 670
Hin6I	GCGC	2 cut(s)	48, 83
HinP1I	GCGC	2 cut(s)	48, 83
HinfI	GANTC	4 cut(s)	262, 474, 524, 670
HpaII	CCGG	3 cut(s)	328, 471, 542
HphI	GGTGA	2 cut(s)	158, 517
Hpy188I	TCNGA	4 cut(s)	35, 462, 529, 708
Hpy188III	TCNNGA	4 cut(s)	10, 12, 554, 694
Hpy99I	CGWCG	4 cut(s)	254, 257, 533, 587
HpyAV	CCTTC	2 cut(s)	622, 679
HpyCH4III	ACNGT	4 cut(s)	63, 119, 193, 700
HpyCH4IV	ACGT	1 cut(s)	531
HpyCH4V	TGCA	3 cut(s)	111, 392, 443
HpyF10VI	GCNNNNNNNGC	2 cut(s)	108, 652
HpyF3I	CTNAG	2 cut(s)	233, 461
HpySE526I	ACGT	1 cut(s)	531
Hsp92II	CATG	2 cut(s)	392, 670
HspAI	GCGC	2 cut(s)	48, 83
KspI	CCGCGG	1 cut(s)	142
Kzo9I	GATC	5 cut(s)	14, 42, 257, 337, 457
LmnI	GCTCC	1 cut(s)	274
LpnPI	CCDG	7 cut(s)	71, 136, 188, 196, 341, 484, 555
Lsp1109I	GCAGC	3 cut(s)	95, 123, 249
LweI	GCATC	2 cut(s)	580, 655
MaeII	ACGT	1 cut(s)	531
MalI	GATC	5 cut(s)	16, 44, 259, 339, 459
MbiI	CCGCTC	1 cut(s)	102
MboI	GATC	5 cut(s)	14, 42, 257, 337, 457
MboII	GAAGA	6 cut(s)	52, 394, 500, 583, 632, 665
MflI	RGATCY	1 cut(s)	42
MluCI	AATT	5 cut(s)	5, 281, 371, 536, 609
MlyI	GAGTC	1 cut(s)	664
MmeI	TCCRAC	1 cut(s)	552
MnlI	CCTC	6 cut(s)	73, 179, 199, 265, 484, 550
MroXI	GAANNNNTTC	1 cut(s)	632
MseI	TTAA	4 cut(s)	195, 225, 495, 612
MslI	CAYNNNNRTG	1 cut(s)	518
MspA1I	CMGCKG	1 cut(s)	141
MspI	CCGG	3 cut(s)	328, 471, 542
MspR9I	CCNGG	1 cut(s)	329
MvnI	CGCG	2 cut(s)	50, 141
MwoI	GCNNNNNNNGC	2 cut(s)	108, 652
NciI	CCSGG	1 cut(s)	329
NdeII	GATC	5 cut(s)	14, 42, 257, 337, 457
NlaIII	CATG	2 cut(s)	392, 670
PaeR7I	CTCGAG	1 cut(s)	10
PdmI	GAANNNNTTC	1 cut(s)	632
PfeI	GAWTC	3 cut(s)	262, 474, 524
PkrI	GCNGC	6 cut(s)	52, 110, 113, 128, 155, 239
PleI	GAGTC	1 cut(s)	664
PpsI	GAGTC	1 cut(s)	664
PstI	CTGCAG	1 cut(s)	113
PsuI	RGATCY	1 cut(s)	42
RseI	CAYNNNNRTG	1 cut(s)	518
SacII	CCGCGG	1 cut(s)	142
SaqAI	TTAA	4 cut(s)	195, 225, 495, 612
SatI	GCNGC	6 cut(s)	51, 109, 112, 127, 154, 238
Sau3AI	GATC	5 cut(s)	14, 42, 257, 337, 457
SchI	GAGTC	1 cut(s)	664
ScrFI	CCNGG	1 cut(s)	329
SfaNI	GCATC	2 cut(s)	580, 655
SfcI	CTRYAG	1 cut(s)	109
Sfr274I	CTCGAG	1 cut(s)	10
Sfr303I	CCGCGG	1 cut(s)	142
SgrBI	CCGCGG	1 cut(s)	142
SlaI	CTCGAG	1 cut(s)	10
SmiMI	CAYNNNNRTG	1 cut(s)	518
SmlI	CTYRAG	2 cut(s)	10, 554
SmoI	CTYRAG	2 cut(s)	10, 554
Sse9I	AATT	5 cut(s)	5, 281, 371, 536, 609
SsiI	CCGC	6 cut(s)	50, 102, 127, 139, 141, 153
StyD4I	CCNGG	1 cut(s)	327
StyI	CCWWGG	1 cut(s)	304
TaaI	ACNGT	4 cut(s)	63, 119, 193, 700
TaiI	ACGT	1 cut(s)	534
TaqI	TCGA	6 cut(s)	11, 17, 242, 249, 260, 435
TasI	AATT	5 cut(s)	5, 281, 371, 536, 609
TauI	GCSGC	3 cut(s)	53, 129, 156
TfiI	GAWTC	3 cut(s)	262, 474, 524
Tru1I	TTAA	4 cut(s)	195, 225, 495, 612
Tru9I	TTAA	4 cut(s)	195, 225, 495, 612
TseI	GCWGC	3 cut(s)	108, 111, 237
TspDTI	ATGAA	4 cut(s)	192, 363, 377, 466
TspGWI	ACGGA	1 cut(s)	270
XapI	RAATTY	2 cut(s)	5, 371
XhoI	CTCGAG	1 cut(s)	10
XmnI	GAANNNNTTC	1 cut(s)	632

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.