RchiOBHm_Chr2g0142851

Calcineurin B-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002018
Physical Location & Seq
Forward (+)
60515720 .. 60518802
3083 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51298

Sequence Viewer

Length: 939 bp
ATGACGACGACGAACACAGAGAGGCAGAGAGCTCTTCTTCTCCGCGATGTTCTGTTGCGCAGGGACATGGACGCCAGGCTCAGCTTTCTGGATTCGAGCACGAGCTCGCTCCAAATCGGCGAGAAGCTGTGTGCGTTGTGCATACCAGTGGTTGGTCTGATCGAGGCTTTCATCTTCAGCTTAGCCGGCTGCTTCGAACAACACCGTCCACGTCGTCACCACCGAGCCGCTTTCACCCACGACGACGTCGTTCGCCTCGCTGCCACCTCCCCATTTAGTGTAAATGACGTGGAAGCATTGCGTGAATTGTTCAACAAGTTGAGCAGTTCGATTCTAGATGATGGCTTAATTCACAAGGAGGAGCTTCAGTTGGCTCTGTTCAAAACTCAAGCAGGCGAAAATATTTTTCTTGATAGGGTATTTGATGTTTTTGACGAAAAGAAAAATGGAGTCATTGAATTTGAGGAGTTTATCCATGCATTAAGTGTATTTCATCCCTGCACTCCTCTTGAACAGAAGATTGACTTTGCATTCAGGTTGTATGATTTGAGACAGACTGGATATATTGAGCGGGAAGAAGTTAGGCAAATGGTAGTCGCCACTTTGTTGGAATCCGGCATACAGGTGCCAGATGAAACTCTAGAAACAATTGTGGATAAGGCAAGGCTCATGAGTGTTCAATCTTTTTTATTTTTTCAGTTTTTCTTTCTGTGTGCTTGCCAAACTTCCCCTGAAATTTTATACTCTGAAAGGCATCTTTCTTTTCTTCAGACATTTGAAGATGCTGATGCTGATAAGGATGGCAAAATTAGCAACGATGAATGGAAGGCTTTTGTATTTCAGCGGCCTACACTCGTAAAGAACATGACACTTCCATATTTAAAGAACATCACCACAGTCTTCCCAAGTTTTATTTTCAACACTGAAGTCGAGGACTGA

Protein Analysis

312

Amino Acids

35.97

Weight (kDa)

5.03

Isoelectric Point (pI)

50.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_5 PF13202 258 - 279 1.2e-06 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 249
Acc16I TGCGCA 1 cut(s) 59
AccB1I GGYRCC 1 cut(s) 625
AccB7I CCANNNNNTGG 1 cut(s) 152
AccBSI CCGCTC 1 cut(s) 571
AccII CGCG 1 cut(s) 45
AciI CCGC 4 cut(s) 43, 228, 571, 844
AcsI RAATTY 2 cut(s) 458, 735
AcuI CTGAAG 3 cut(s) 160, 350, 752
AcyI GRCGYC 2 cut(s) 72, 246
AfiI CCNNNNNNNGG 1 cut(s) 152
AgsI TTSAA 7 cut(s) 313, 382, 458, 512, 680, 779, 919
AjiI CACGTC 2 cut(s) 212, 289
AjnI CCWGG 1 cut(s) 74
AluBI AGCT 6 cut(s) 32, 84, 105, 127, 180, 364
AluI AGCT 6 cut(s) 32, 84, 105, 127, 180, 364
Alw21I GWGCWC 3 cut(s) 34, 101, 107
Alw26I GTCTC 1 cut(s) 544
AoxI GGCC 1 cut(s) 845
ApeKI GCWGC 2 cut(s) 189, 260
ApoI RAATTY 2 cut(s) 458, 735
AspLEI GCGC 1 cut(s) 60
AsuHPI GGTGA 3 cut(s) 209, 226, 883
AsuII TTCGAA 1 cut(s) 195
BanI GGYRCC 1 cut(s) 625
BanII GRGCYC 2 cut(s) 34, 107
BauI CACGAG 1 cut(s) 100
BbsI GAAGAC 1 cut(s) 892
Bbv12I GWGCWC 3 cut(s) 34, 101, 107
BbvI GCAGC 2 cut(s) 176, 247
BccI CCATC 2 cut(s) 335, 794
BciT130I CCWGG 1 cut(s) 76
BcoDI GTCTC 1 cut(s) 544
BfaI CTAG 2 cut(s) 335, 641
BisI GCNGC 4 cut(s) 190, 228, 261, 845
BlpI GCTNAGC 2 cut(s) 80, 181
BlsI GCNGC 4 cut(s) 191, 229, 262, 846
Bme1390I CCNGG 1 cut(s) 76
BmgBI CACGTC 2 cut(s) 212, 289
BmiI GGNNCC 1 cut(s) 627
BmrFI CCNGG 1 cut(s) 76
BmsI GCATC 3 cut(s) 763, 772, 778
BpiI GAAGAC 1 cut(s) 892
Bpu1102I GCTNAGC 2 cut(s) 80, 181
Bpu14I TTCGAA 1 cut(s) 195
BpuEI CTTGAG 1 cut(s) 372
BsaHI GRCGYC 2 cut(s) 72, 246
BsaXI ACNNNNNCTCC 2 cut(s) 353, 383
Bsc4I CCNNNNNNNGG 1 cut(s) 152
Bse118I RCCGGY 1 cut(s) 185
Bse1I ACTGG 2 cut(s) 146, 562
Bse3DI GCAATG 1 cut(s) 296
BseBI CCWGG 1 cut(s) 76
BseGI GGATG 2 cut(s) 493, 805
BseLI CCNNNNNNNGG 1 cut(s) 152
BseMI GCAATG 1 cut(s) 296
BseMII CTCAG 1 cut(s) 94
BseNI ACTGG 2 cut(s) 146, 562
BseRI GAGGAG 3 cut(s) 374, 479, 495
BseXI GCAGC 2 cut(s) 176, 247
BsgI GTGCAG 1 cut(s) 484
Bsh1236I CGCG 1 cut(s) 45
BshFI GGCC 1 cut(s) 847
BshNI GGYRCC 1 cut(s) 625
BsiHKAI GWGCWC 3 cut(s) 34, 101, 107
BsiSI CCGG 2 cut(s) 186, 615
BslFI GGGAC 1 cut(s) 77
BslI CCNNNNNNNGG 1 cut(s) 152
BsmAI GTCTC 1 cut(s) 544
BsmFI GGGAC 1 cut(s) 77
BsmI GAATGC 1 cut(s) 530
BsnI GGCC 1 cut(s) 847
Bsp119I TTCGAA 1 cut(s) 195
Bsp1286I GDGCHC 3 cut(s) 34, 101, 107
Bsp143I GATC 1 cut(s) 159
Bsp1720I GCTNAGC 2 cut(s) 80, 181
BspACI CCGC 4 cut(s) 43, 228, 571, 844
BspANI GGCC 1 cut(s) 847
BspCNI CTCAG 1 cut(s) 93
BspFNI CGCG 1 cut(s) 45
BspHI TCATGA 1 cut(s) 669
BspLI GGNNCC 1 cut(s) 627
BspQI GCTCTTC 1 cut(s) 39
BspT104I TTCGAA 1 cut(s) 195
BspT107I GGYRCC 1 cut(s) 625
BsrBI CCGCTC 1 cut(s) 571
BsrDI GCAATG 1 cut(s) 296
BsrFI RCCGGY 1 cut(s) 185
BsrI ACTGG 2 cut(s) 146, 562
BssAI RCCGGY 1 cut(s) 185
BssMI GATC 1 cut(s) 159
BssNI GRCGYC 2 cut(s) 72, 246
BssSI CACGAG 1 cut(s) 100
Bst2BI CACGAG 1 cut(s) 100
Bst2UI CCWGG 1 cut(s) 76
Bst4CI ACNGT 2 cut(s) 206, 898
Bst6I CTCTTC 1 cut(s) 39
BstACI GRCGYC 2 cut(s) 72, 246
BstBI TTCGAA 1 cut(s) 195
BstC8I GCNNGC 4 cut(s) 107, 187, 394, 718
BstDEI CTNAG 2 cut(s) 80, 181
BstF5I GGATG 2 cut(s) 493, 805
BstFNI CGCG 1 cut(s) 45
BstHHI GCGC 1 cut(s) 60
BstKTI GATC 1 cut(s) 162
BstMAI GTCTC 1 cut(s) 544
BstMBI GATC 1 cut(s) 159
BstMWI GCNNNNNNNGC 2 cut(s) 186, 810
BstNI CCWGG 1 cut(s) 76
BstSCI CCNGG 1 cut(s) 74
BstUI CGCG 1 cut(s) 45
BstV1I GCAGC 2 cut(s) 176, 247
BstV2I GAAGAC 1 cut(s) 892
BstXI CCANNNNNNTGG 1 cut(s) 607
BsuRI GGCC 1 cut(s) 847
BtgZI GCGATG 1 cut(s) 60
BtrI CACGTC 2 cut(s) 212, 289
BtsCI GGATG 2 cut(s) 493, 805
BtsIMutI CAGTG 2 cut(s) 153, 921
Cac8I GCNNGC 4 cut(s) 107, 187, 394, 718
CciI TCATGA 1 cut(s) 669
CfoI GCGC 1 cut(s) 60
Cfr10I RCCGGY 1 cut(s) 185
CseI GACGC 1 cut(s) 80
CviAII CATG 4 cut(s) 67, 476, 670, 865
DdeI CTNAG 2 cut(s) 80, 181
DpnI GATC 1 cut(s) 161
DpnII GATC 1 cut(s) 159
DraI TTTAAA 1 cut(s) 882
Eam1104I CTCTTC 1 cut(s) 39
EarI CTCTTC 1 cut(s) 39
Ecl136II GAGCTC 2 cut(s) 32, 105
Eco24I GRGCYC 2 cut(s) 34, 107
Eco53kI GAGCTC 2 cut(s) 32, 105
Eco57I CTGAAG 3 cut(s) 160, 350, 752
EcoICRI GAGCTC 2 cut(s) 32, 105
EcoRII CCWGG 1 cut(s) 74
EcoT22I ATGCAT 1 cut(s) 481
EcoT38I GRGCYC 2 cut(s) 34, 107
FaeI CATG 4 cut(s) 70, 479, 673, 868
FalI AAGNNNNNCTT 2 cut(s) 509, 541
FaqI GGGAC 1 cut(s) 77
FatI CATG 4 cut(s) 66, 475, 669, 864
FauI CCCGC 1 cut(s) 564
Fnu4HI GCNGC 4 cut(s) 190, 228, 261, 845
FokI GGATG 2 cut(s) 480, 812
FriOI GRGCYC 2 cut(s) 34, 107
Fsp4HI GCNGC 4 cut(s) 190, 228, 261, 845
FspBI CTAG 2 cut(s) 335, 641
FspI TGCGCA 1 cut(s) 59
GlaI GCGC 1 cut(s) 59
GluI GCNGC 4 cut(s) 190, 228, 261, 845
HaeIII GGCC 1 cut(s) 847
HapII CCGG 2 cut(s) 186, 615
HgaI GACGC 1 cut(s) 80
HhaI GCGC 1 cut(s) 60
Hin1I GRCGYC 2 cut(s) 72, 246
Hin1II CATG 4 cut(s) 70, 479, 673, 868
Hin6I GCGC 1 cut(s) 58
HinP1I GCGC 1 cut(s) 58
HinfI GANTC 4 cut(s) 92, 331, 450, 611
HpaII CCGG 2 cut(s) 186, 615
HphI GGTGA 3 cut(s) 209, 226, 883
Hpy166II GTNNAC 1 cut(s) 209
Hpy188I TCNGA 3 cut(s) 159, 748, 771
Hpy188III TCNNGA 6 cut(s) 89, 335, 410, 509, 641, 670
Hpy8I GTNNAC 1 cut(s) 209
Hpy99I CGWCG 6 cut(s) 10, 13, 216, 245, 248, 251
HpyAV CCTTC 1 cut(s) 820
HpyCH4III ACNGT 2 cut(s) 206, 898
HpyCH4IV ACGT 3 cut(s) 211, 246, 288
HpyCH4V TGCA 4 cut(s) 141, 479, 501, 530
HpyF10VI GCNNNNNNNGC 2 cut(s) 186, 810
HpyF3I CTNAG 2 cut(s) 80, 181
HpySE526I ACGT 3 cut(s) 211, 246, 288
Hsp92I GRCGYC 2 cut(s) 72, 246
Hsp92II CATG 4 cut(s) 70, 479, 673, 868
HspAI GCGC 1 cut(s) 58
KroI GCCGGC 1 cut(s) 185
KroNI GCCGGC 1 cut(s) 187
Kzo9I GATC 1 cut(s) 159
LguI GCTCTTC 1 cut(s) 39
LmnI GCTCC 2 cut(s) 114, 361
Lsp1109I GCAGC 2 cut(s) 176, 247
LweI GCATC 3 cut(s) 763, 772, 778
MaeI CTAG 2 cut(s) 335, 641
MaeII ACGT 3 cut(s) 211, 246, 288
MaeIII GTNAC 1 cut(s) 215
MalI GATC 1 cut(s) 161
MbiI CCGCTC 1 cut(s) 571
MboI GATC 1 cut(s) 159
MboII GAAGA 8 cut(s) 26, 29, 166, 529, 587, 758, 791, 892
MfeI CAATTG 1 cut(s) 648
MhlI GDGCHC 3 cut(s) 34, 101, 107
MluCI AATT 6 cut(s) 305, 348, 458, 648, 735, 807
MlyI GAGTC 1 cut(s) 459
MmeI TCCRAC 1 cut(s) 588
MnlI CCTC 8 cut(s) 15, 157, 266, 277, 352, 457, 516, 925
Mph1103I ATGCAT 1 cut(s) 481
MroNI GCCGGC 1 cut(s) 185
MseI TTAA 3 cut(s) 347, 482, 881
MslI CAYNNNNRTG 2 cut(s) 146, 623
MspA1I CMGCKG 1 cut(s) 844
MspI CCGG 2 cut(s) 186, 615
MspR9I CCNGG 1 cut(s) 76
MunI CAATTG 1 cut(s) 648
Mva1269I GAATGC 1 cut(s) 530
MvaI CCWGG 1 cut(s) 76
MvnI CGCG 1 cut(s) 45
MwoI GCNNNNNNNGC 2 cut(s) 186, 810
NaeI GCCGGC 1 cut(s) 187
NdeII GATC 1 cut(s) 159
NgoMIV GCCGGC 1 cut(s) 185
NlaIII CATG 4 cut(s) 70, 479, 673, 868
NlaIV GGNNCC 1 cut(s) 627
NmuCI GTSAC 1 cut(s) 215
NsbI TGCGCA 1 cut(s) 59
NsiI ATGCAT 1 cut(s) 481
NspV TTCGAA 1 cut(s) 195
PagI TCATGA 1 cut(s) 669
PciSI GCTCTTC 1 cut(s) 39
PcsI WCGNNNNNNNCGW 2 cut(s) 220, 246
PctI GAATGC 1 cut(s) 530
PdiI GCCGGC 1 cut(s) 187
PfeI GAWTC 3 cut(s) 92, 331, 611
PflFI GACNNNGTC 1 cut(s) 245
PflMI CCANNNNNTGG 1 cut(s) 152
PkrI GCNGC 4 cut(s) 191, 229, 262, 846
PleI GAGTC 1 cut(s) 458
PpsI GAGTC 1 cut(s) 458
Psp124BI GAGCTC 2 cut(s) 34, 107
Psp6I CCWGG 1 cut(s) 74
PspGI CCWGG 1 cut(s) 74
PspN4I GGNNCC 1 cut(s) 627
PsyI GACNNNGTC 1 cut(s) 245
RseI CAYNNNNRTG 2 cut(s) 146, 623
SacI GAGCTC 2 cut(s) 34, 107
SapI GCTCTTC 1 cut(s) 39
SaqAI TTAA 3 cut(s) 347, 482, 881
SatI GCNGC 4 cut(s) 190, 228, 261, 845
Sau3AI GATC 1 cut(s) 159
SchI GAGTC 1 cut(s) 459
ScrFI CCNGG 1 cut(s) 76
SduI GDGCHC 3 cut(s) 34, 101, 107
SfaNI GCATC 3 cut(s) 763, 772, 778
SfuI TTCGAA 1 cut(s) 195
SmiMI CAYNNNNRTG 2 cut(s) 146, 623
SmlI CTYRAG 1 cut(s) 387
SmoI CTYRAG 1 cut(s) 387
Sse9I AATT 6 cut(s) 305, 348, 458, 648, 735, 807
SsiI CCGC 4 cut(s) 43, 228, 571, 844
SspI AATATT 1 cut(s) 403
SspMI CTAG 2 cut(s) 335, 641
SstI GAGCTC 2 cut(s) 34, 107
StyD4I CCNGG 1 cut(s) 74
TaaI ACNGT 2 cut(s) 206, 898
TaiI ACGT 3 cut(s) 214, 249, 291
TaqI TCGA 5 cut(s) 95, 162, 195, 329, 930
TasI AATT 6 cut(s) 305, 348, 458, 648, 735, 807
TauI GCSGC 2 cut(s) 230, 847
TfiI GAWTC 3 cut(s) 92, 331, 611
Tru1I TTAA 3 cut(s) 347, 482, 881
Tru9I TTAA 3 cut(s) 347, 482, 881
TscAI CASTG 2 cut(s) 153, 928
TseFI GTSAC 1 cut(s) 215
TseI GCWGC 2 cut(s) 189, 260
Tsp45I GTSAC 1 cut(s) 215
TspDTI ATGAA 4 cut(s) 160, 482, 648, 834
TspRI CASTG 2 cut(s) 153, 928
Tth111I GACNNNGTC 1 cut(s) 245
Van91I CCANNNNNTGG 1 cut(s) 152
XapI RAATTY 2 cut(s) 458, 735
XbaI TCTAGA 2 cut(s) 334, 640
XspI CTAG 2 cut(s) 335, 641
ZraI GACGTC 1 cut(s) 247
Zsp2I ATGCAT 1 cut(s) 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.