FvH4_6g43100

2-alkenal reductase (NADP( )-dependent)-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0003278
Physical Location & Seq
Reverse (-)
33393700 .. 33394970
1271 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g43100.t1

Sequence Viewer

Length: 402 bp
ATGAGTGGTACAGTAGTCGAAAGCAGAGAATGGTACATGGCTGCTTATGCTCCTGAAGGTATTCCAACTTCAGATCATCTTAAACTTCGCTGCGTCTCACTGGCCCTTGATGCAATCCCTGAACAACATGTCGTCCTTGAGACGCTCTTCGTCTCCGTTGAACCATACCTGCGTGGCCGAATGACAGGCCTGGACGAAGGGCTGTACTTTGCGCAGTTCAAACTTAACGAGGTAATTTCATCATTTGGGGTTGGAAGAGTAATTAGGTCGAAGGATAATGACTATGGGCTACCTGGGTTGGCAGCGTGGGTGGGAATAGAGGTGATCGCGGCTCCGATAAAAGCTGGGTCGAATGTTTTCATCTCAGCCGCAGCAAGTGCAGTTGGGATCTATGCAGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N_2 ADH_zinc_N

Protein Analysis

134

Amino Acids

14.25

Weight (kDa)

5.06

Isoelectric Point (pI)

31.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N_2 PF16884 10 - 95 8.4e-14 N-terminal domain of oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 213
Acc36I ACCTGC 1 cut(s) 177
AccII CGCG 1 cut(s) 329
AciI CCGC 2 cut(s) 329, 369
AclWI GGATC 1 cut(s) 395
AcoI YGGCCR 1 cut(s) 175
AcuI CTGAAG 2 cut(s) 54, 75
AfaI GTAC 3 cut(s) 10, 35, 206
AflIII ACRYGT 1 cut(s) 127
AgsI TTSAA 2 cut(s) 161, 220
AjnI CCWGG 2 cut(s) 189, 292
AluBI AGCT 1 cut(s) 344
AluI AGCT 1 cut(s) 344
Alw26I GTCTC 3 cut(s) 100, 134, 157
AlwI GGATC 1 cut(s) 395
AoxI GGCC 3 cut(s) 102, 175, 187
ApeKI GCWGC 4 cut(s) 41, 90, 302, 371
Asp700I GAANNNNTTC 2 cut(s) 60, 356
AspLEI GCGC 1 cut(s) 214
AspS9I GGNCC 1 cut(s) 103
AsuHPI GGTGA 1 cut(s) 334
BaeI ACNNNNGTAYC 1 cut(s) 33
BarI GAAGNNNNNNTAC 2 cut(s) 52, 84
BbvI GCAGC 4 cut(s) 28, 77, 314, 383
BciT130I CCWGG 2 cut(s) 191, 294
BcoDI GTCTC 3 cut(s) 100, 134, 157
BfuAI ACCTGC 1 cut(s) 177
BisI GCNGC 6 cut(s) 42, 91, 303, 330, 369, 372
BlsI GCNGC 6 cut(s) 43, 92, 304, 331, 370, 373
Bme1390I CCNGG 2 cut(s) 191, 294
BmgT120I GGNCC 1 cut(s) 103
BmiI GGNNCC 1 cut(s) 333
BmrFI CCNGG 2 cut(s) 191, 294
BmsI GCATC 1 cut(s) 100
BpuEI CTTGAG 1 cut(s) 158
BsaJI CCNNGG 1 cut(s) 293
Bse1I ACTGG 1 cut(s) 105
BseBI CCWGG 2 cut(s) 191, 294
BseDI CCNNGG 1 cut(s) 293
BseMII CTCAG 1 cut(s) 378
BseNI ACTGG 1 cut(s) 105
BseXI GCAGC 4 cut(s) 28, 77, 314, 383
BseYI CCCAGC 1 cut(s) 344
BsgI GTGCAG 1 cut(s) 399
Bsh1236I CGCG 1 cut(s) 329
BshFI GGCC 3 cut(s) 104, 177, 189
BsmAI GTCTC 3 cut(s) 100, 134, 157
BsmBI CGTCTC 3 cut(s) 100, 134, 157
BsnI GGCC 3 cut(s) 104, 177, 189
Bsp143I GATC 3 cut(s) 73, 324, 387
BspACI CCGC 2 cut(s) 329, 369
BspANI GGCC 3 cut(s) 104, 177, 189
BspCNI CTCAG 1 cut(s) 377
BspFNI CGCG 1 cut(s) 329
BspLI GGNNCC 1 cut(s) 333
BspMI ACCTGC 1 cut(s) 177
BspPI GGATC 1 cut(s) 395
BspQI GCTCTTC 1 cut(s) 152
BsrI ACTGG 1 cut(s) 105
BssECI CCNNGG 1 cut(s) 293
BssMI GATC 3 cut(s) 73, 324, 387
Bst2UI CCWGG 2 cut(s) 191, 294
Bst4CI ACNGT 1 cut(s) 13
Bst6I CTCTTC 2 cut(s) 152, 250
BstAPI GCANNNNNTGC 1 cut(s) 377
BstDEI CTNAG 1 cut(s) 364
BstFNI CGCG 1 cut(s) 329
BstHHI GCGC 1 cut(s) 214
BstKTI GATC 3 cut(s) 76, 327, 390
BstMAI GTCTC 3 cut(s) 100, 134, 157
BstMBI GATC 3 cut(s) 73, 324, 387
BstMWI GCNNNNNNNGC 3 cut(s) 47, 110, 377
BstNI CCWGG 2 cut(s) 191, 294
BstNSI RCATGY 1 cut(s) 131
BstSCI CCNGG 2 cut(s) 189, 292
BstUI CGCG 1 cut(s) 329
BstV1I GCAGC 4 cut(s) 28, 77, 314, 383
BstX2I RGATCY 1 cut(s) 387
BstYI RGATCY 1 cut(s) 387
BsuRI GGCC 3 cut(s) 104, 177, 189
BtsIMutI CAGTG 1 cut(s) 98
BveI ACCTGC 1 cut(s) 177
CfoI GCGC 1 cut(s) 214
Cfr13I GGNCC 1 cut(s) 103
CseI GACGC 2 cut(s) 82, 151
Csp6I GTAC 3 cut(s) 9, 34, 205
CviAII CATG 2 cut(s) 37, 128
CviJI RGCY 9 cut(s) 41, 104, 177, 189, 202, 289, 332, 344, 368
CviKI_1 RGCY 9 cut(s) 41, 104, 177, 189, 202, 289, 332, 344, 368
CviQI GTAC 3 cut(s) 9, 34, 205
DdeI CTNAG 1 cut(s) 364
DpnI GATC 3 cut(s) 75, 326, 389
DpnII GATC 3 cut(s) 73, 324, 387
EaeI YGGCCR 1 cut(s) 175
Eam1104I CTCTTC 2 cut(s) 152, 250
EarI CTCTTC 2 cut(s) 152, 250
Eco147I AGGCCT 1 cut(s) 189
Eco57I CTGAAG 2 cut(s) 54, 75
EcoRII CCWGG 2 cut(s) 189, 292
Esp3I CGTCTC 3 cut(s) 100, 134, 157
FaeI CATG 2 cut(s) 40, 131
FaiI YATR 6 cut(s) 38, 48, 129, 166, 285, 393
FatI CATG 2 cut(s) 36, 127
Fnu4HI GCNGC 6 cut(s) 42, 91, 303, 330, 369, 372
Fsp4HI GCNGC 6 cut(s) 42, 91, 303, 330, 369, 372
FspI TGCGCA 1 cut(s) 213
GlaI GCGC 1 cut(s) 213
GluI GCNGC 6 cut(s) 42, 91, 303, 330, 369, 372
GsaI CCCAGC 1 cut(s) 348
HaeIII GGCC 3 cut(s) 104, 177, 189
HgaI GACGC 2 cut(s) 82, 151
HhaI GCGC 1 cut(s) 214
Hin1II CATG 2 cut(s) 40, 131
Hin6I GCGC 1 cut(s) 212
HinP1I GCGC 1 cut(s) 212
HphI GGTGA 1 cut(s) 334
Hpy188I TCNGA 2 cut(s) 73, 336
Hpy188III TCNNGA 1 cut(s) 53
HpyAV CCTTC 3 cut(s) 50, 191, 265
HpyCH4III ACNGT 1 cut(s) 13
HpyCH4V TGCA 3 cut(s) 113, 380, 395
HpyF10VI GCNNNNNNNGC 3 cut(s) 47, 110, 377
HpyF3I CTNAG 1 cut(s) 364
Hsp92II CATG 2 cut(s) 40, 131
HspAI GCGC 1 cut(s) 212
Kzo9I GATC 3 cut(s) 73, 324, 387
LguI GCTCTTC 1 cut(s) 152
LmnI GCTCC 2 cut(s) 55, 337
Lsp1109I GCAGC 4 cut(s) 28, 77, 314, 383
LweI GCATC 1 cut(s) 100
MalI GATC 3 cut(s) 75, 326, 389
MboI GATC 3 cut(s) 73, 324, 387
MboII GAAGA 2 cut(s) 139, 267
MflI RGATCY 1 cut(s) 387
MluCI AATT 2 cut(s) 234, 261
MmeI TCCRAC 2 cut(s) 89, 232
MnlI CCTC 2 cut(s) 223, 313
MroXI GAANNNNTTC 2 cut(s) 60, 356
MseI TTAA 2 cut(s) 81, 225
MspR9I CCNGG 2 cut(s) 191, 294
MvaI CCWGG 2 cut(s) 191, 294
MvnI CGCG 1 cut(s) 329
MwoI GCNNNNNNNGC 3 cut(s) 47, 110, 377
NdeII GATC 3 cut(s) 73, 324, 387
NlaIII CATG 2 cut(s) 40, 131
NlaIV GGNNCC 1 cut(s) 333
NsbI TGCGCA 1 cut(s) 213
NspI RCATGY 1 cut(s) 131
PceI AGGCCT 1 cut(s) 189
PciI ACATGT 1 cut(s) 127
PciSI GCTCTTC 1 cut(s) 152
PdmI GAANNNNTTC 2 cut(s) 60, 356
PkrI GCNGC 6 cut(s) 43, 92, 304, 331, 370, 373
PscI ACATGT 1 cut(s) 127
Psp6I CCWGG 2 cut(s) 189, 292
PspFI CCCAGC 1 cut(s) 344
PspGI CCWGG 2 cut(s) 189, 292
PspN4I GGNNCC 1 cut(s) 333
PspPI GGNCC 1 cut(s) 103
PsuI RGATCY 1 cut(s) 387
RsaI GTAC 3 cut(s) 10, 35, 206
RsaNI GTAC 3 cut(s) 9, 34, 205
SapI GCTCTTC 1 cut(s) 152
SaqAI TTAA 2 cut(s) 81, 225
SatI GCNGC 6 cut(s) 42, 91, 303, 330, 369, 372
Sau3AI GATC 3 cut(s) 73, 324, 387
Sau96I GGNCC 1 cut(s) 103
ScrFI CCNGG 2 cut(s) 191, 294
SetI ASST 7 cut(s) 61, 171, 234, 269, 295, 324, 346
SfaNI GCATC 1 cut(s) 100
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
Sse9I AATT 2 cut(s) 234, 261
SseBI AGGCCT 1 cut(s) 189
SsiI CCGC 2 cut(s) 329, 369
StuI AGGCCT 1 cut(s) 189
StyD4I CCNGG 2 cut(s) 189, 292
TaaI ACNGT 1 cut(s) 13
TaqI TCGA 3 cut(s) 18, 269, 350
TasI AATT 2 cut(s) 234, 261
TatI WGTACW 1 cut(s) 204
TauI GCSGC 2 cut(s) 332, 371
Tru1I TTAA 2 cut(s) 81, 225
Tru9I TTAA 2 cut(s) 81, 225
TscAI CASTG 1 cut(s) 105
TseI GCWGC 4 cut(s) 41, 90, 302, 371
TspDTI ATGAA 2 cut(s) 228, 349
TspGWI ACGGA 1 cut(s) 145
TspRI CASTG 1 cut(s) 105
XceI RCATGY 1 cut(s) 131
XmnI GAANNNNTTC 2 cut(s) 60, 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.