Rmu_co8366625.1_g000001

2-alkenal reductase (NADP( )-dependent)-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8366625.1
OG0003278
Physical Location & Seq
Forward (+)
1 .. 753
753 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8366625.1_g000001.1.cds

Sequence Viewer

Length: 515 bp
ttgatgcaatccctgaacaacatgtcgtccttgagacgcttttcgtgtccgttgaaccatacctgcgtggcagaatgaccggcctggaagaagggctatactttgcgcagttcaaccttaacgaggtagtttcagcatttggtgttggaagagtaattatgtcaaaggatagtgactatggtgaaggtgacatagtgatctgtccttccacatcttttgccgagtactgtgtggtatcatcccagaatttaagaaaggtggagccaaatgctgggattccattgcctgaatatctcggtttgttagggatacctgggttggcagcgtgggtgggaatagaggtgatcgcggagccaataaaagcagggtcgaatgtgttcatatcagctgcggccagtgccgttgggatttatgcagggcagttggccaagcttaaaggctgtcgagtagttggaagcactggaactgacgacaaggtaagaaattcgttgagtacgtattattgcgattgttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N_2 ADH_zinc_N

Protein Analysis

170

Amino Acids

18.09

Weight (kDa)

4.66

Isoelectric Point (pI)

23.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 107
Acc36I ACCTGC 1 cut(s) 71
AccB7I CCANNNNNTGG 1 cut(s) 271
AccII CGCG 1 cut(s) 349
AciI CCGC 2 cut(s) 349, 391
AcoI YGGCCR 2 cut(s) 392, 425
AcsI RAATTY 2 cut(s) 246, 483
AfaI GTAC 2 cut(s) 226, 495
AfiI CCNNNNNNNGG 2 cut(s) 123, 271
AflIII ACRYGT 1 cut(s) 21
AgsI TTSAA 2 cut(s) 55, 114
AjnI CCWGG 2 cut(s) 83, 312
AluBI AGCT 2 cut(s) 388, 432
AluI AGCT 2 cut(s) 388, 432
Alw26I GTCTC 1 cut(s) 28
AoxI GGCC 3 cut(s) 81, 392, 425
ApeKI GCWGC 2 cut(s) 322, 388
ApoI RAATTY 2 cut(s) 246, 483
Asp700I GAANNNNTTC 1 cut(s) 376
AspLEI GCGC 1 cut(s) 108
AsuHPI GGTGA 3 cut(s) 193, 199, 354
BaeI ACNNNNGTAYC 2 cut(s) 301, 334
BalI TGGCCA 1 cut(s) 427
BbvI GCAGC 2 cut(s) 334, 375
BceAI ACGGC 1 cut(s) 385
BciT130I CCWGG 2 cut(s) 85, 314
BciVI GTATCC 1 cut(s) 302
BcoDI GTCTC 1 cut(s) 28
BfuAI ACCTGC 1 cut(s) 71
BfuI GTATCC 1 cut(s) 302
BisI GCNGC 3 cut(s) 323, 389, 392
BlsI GCNGC 3 cut(s) 324, 390, 393
BmcAI AGTACT 1 cut(s) 226
Bme1390I CCNGG 2 cut(s) 85, 314
BmiI GGNNCC 2 cut(s) 263, 353
BmrFI CCNGG 2 cut(s) 85, 314
BpuEI CTTGAG 1 cut(s) 52
BsaAI YACGTR 1 cut(s) 497
BsaJI CCNNGG 1 cut(s) 313
Bsc4I CCNNNNNNNGG 2 cut(s) 123, 271
Bse118I RCCGGY 1 cut(s) 79
Bse1I ACTGG 2 cut(s) 395, 465
Bse3DI GCAATG 1 cut(s) 280
BseBI CCWGG 2 cut(s) 85, 314
BseDI CCNNGG 1 cut(s) 313
BseGI GGATG 1 cut(s) 238
BseLI CCNNNNNNNGG 2 cut(s) 123, 271
BseMI GCAATG 1 cut(s) 280
BseNI ACTGG 2 cut(s) 395, 465
BseXI GCAGC 2 cut(s) 334, 375
BseYI CCCAGC 1 cut(s) 271
Bsh1236I CGCG 1 cut(s) 349
BshFI GGCC 3 cut(s) 83, 394, 427
BsiSI CCGG 1 cut(s) 80
BslI CCNNNNNNNGG 2 cut(s) 123, 271
BsmAI GTCTC 1 cut(s) 28
BsmBI CGTCTC 1 cut(s) 28
BsnI GGCC 3 cut(s) 83, 394, 427
Bsp143I GATC 2 cut(s) 197, 344
BspACI CCGC 2 cut(s) 349, 391
BspANI GGCC 3 cut(s) 83, 394, 427
BspFNI CGCG 1 cut(s) 349
BspLI GGNNCC 2 cut(s) 263, 353
BspMI ACCTGC 1 cut(s) 71
BsrDI GCAATG 1 cut(s) 280
BsrFI RCCGGY 1 cut(s) 79
BsrI ACTGG 2 cut(s) 395, 465
BssAI RCCGGY 1 cut(s) 79
BssECI CCNNGG 1 cut(s) 313
BssMI GATC 2 cut(s) 197, 344
Bst2UI CCWGG 2 cut(s) 85, 314
Bst4CI ACNGT 1 cut(s) 229
Bst6I CTCTTC 1 cut(s) 144
BstBAI YACGTR 1 cut(s) 497
BstENI CCTNNNNNAGG 1 cut(s) 121
BstF5I GGATG 1 cut(s) 238
BstFNI CGCG 1 cut(s) 349
BstHHI GCGC 1 cut(s) 108
BstKTI GATC 2 cut(s) 200, 347
BstMAI GTCTC 1 cut(s) 28
BstMBI GATC 2 cut(s) 197, 344
BstMWI GCNNNNNNNGC 1 cut(s) 397
BstNI CCWGG 2 cut(s) 85, 314
BstNSI RCATGY 1 cut(s) 25
BstSCI CCNGG 2 cut(s) 83, 312
BstSNI TACGTA 1 cut(s) 497
BstUI CGCG 1 cut(s) 349
BstV1I GCAGC 2 cut(s) 334, 375
BsuI GTATCC 1 cut(s) 302
BsuRI GGCC 3 cut(s) 83, 394, 427
BtsCI GGATG 1 cut(s) 238
BtsIMutI CAGTG 2 cut(s) 402, 458
BveI ACCTGC 1 cut(s) 71
CfoI GCGC 1 cut(s) 108
Cfr10I RCCGGY 1 cut(s) 79
CseI GACGC 1 cut(s) 45
Csp6I GTAC 2 cut(s) 225, 494
CspCI CAANNNNNGTGG 2 cut(s) 198, 233
CviAII CATG 1 cut(s) 22
CviJI RGCY 9 cut(s) 83, 96, 264, 354, 388, 394, 427, 432, 440
CviKI_1 RGCY 9 cut(s) 83, 96, 264, 354, 388, 394, 427, 432, 440
CviQI GTAC 2 cut(s) 225, 494
DpnI GATC 2 cut(s) 199, 346
DpnII GATC 2 cut(s) 197, 344
EaeI YGGCCR 2 cut(s) 392, 425
Eam1104I CTCTTC 1 cut(s) 144
EarI CTCTTC 1 cut(s) 144
Eco105I TACGTA 1 cut(s) 497
EcoNI CCTNNNNNAGG 1 cut(s) 121
EcoRII CCWGG 2 cut(s) 83, 312
Esp3I CGTCTC 1 cut(s) 28
FaeI CATG 1 cut(s) 25
FaiI YATR 8 cut(s) 23, 60, 99, 160, 179, 193, 382, 413
FatI CATG 1 cut(s) 21
Fnu4HI GCNGC 3 cut(s) 323, 389, 392
FokI GGATG 1 cut(s) 225
Fsp4HI GCNGC 3 cut(s) 323, 389, 392
FspI TGCGCA 1 cut(s) 107
GlaI GCGC 1 cut(s) 107
GluI GCNGC 3 cut(s) 323, 389, 392
GsaI CCCAGC 1 cut(s) 275
HaeIII GGCC 3 cut(s) 83, 394, 427
HapII CCGG 1 cut(s) 80
HgaI GACGC 1 cut(s) 45
HhaI GCGC 1 cut(s) 108
Hin1II CATG 1 cut(s) 25
Hin6I GCGC 1 cut(s) 106
HinP1I GCGC 1 cut(s) 106
HindIII AAGCTT 1 cut(s) 430
HinfI GANTC 1 cut(s) 276
HpaII CCGG 1 cut(s) 80
HphI GGTGA 3 cut(s) 193, 199, 354
HpyAV CCTTC 3 cut(s) 85, 178, 215
HpyCH4III ACNGT 1 cut(s) 229
HpyCH4IV ACGT 1 cut(s) 496
HpyCH4V TGCA 2 cut(s) 7, 415
HpyF10VI GCNNNNNNNGC 1 cut(s) 397
HpySE526I ACGT 1 cut(s) 496
Hsp92II CATG 1 cut(s) 25
HspAI GCGC 1 cut(s) 106
Kzo9I GATC 2 cut(s) 197, 344
LmnI GCTCC 2 cut(s) 261, 351
Lsp1109I GCAGC 2 cut(s) 334, 375
MaeII ACGT 1 cut(s) 496
MaeIII GTNAC 2 cut(s) 172, 187
MalI GATC 2 cut(s) 199, 346
MboI GATC 2 cut(s) 197, 344
MboII GAAGA 2 cut(s) 100, 161
MlsI TGGCCA 1 cut(s) 427
MluCI AATT 3 cut(s) 155, 246, 483
MluNI TGGCCA 1 cut(s) 427
MmeI TCCRAC 2 cut(s) 126, 432
MnlI CCTC 2 cut(s) 117, 333
Mox20I TGGCCA 1 cut(s) 427
MroXI GAANNNNTTC 1 cut(s) 376
MscI TGGCCA 1 cut(s) 427
MseI TTAA 3 cut(s) 119, 250, 434
Msp20I TGGCCA 1 cut(s) 427
MspA1I CMGCKG 1 cut(s) 388
MspI CCGG 1 cut(s) 80
MspR9I CCNGG 2 cut(s) 85, 314
MvaI CCWGG 2 cut(s) 85, 314
MvnI CGCG 1 cut(s) 349
MwoI GCNNNNNNNGC 1 cut(s) 397
NdeII GATC 2 cut(s) 197, 344
NlaIII CATG 1 cut(s) 25
NlaIV GGNNCC 2 cut(s) 263, 353
NmeAIII GCCGAG 1 cut(s) 246
NmuCI GTSAC 2 cut(s) 172, 187
NsbI TGCGCA 1 cut(s) 107
NspI RCATGY 1 cut(s) 25
PciI ACATGT 1 cut(s) 21
PcsI WCGNNNNNNNCGW 1 cut(s) 493
PdmI GAANNNNTTC 1 cut(s) 376
PfeI GAWTC 1 cut(s) 276
PflMI CCANNNNNTGG 1 cut(s) 271
PkrI GCNGC 3 cut(s) 324, 390, 393
Ppu21I YACGTR 1 cut(s) 497
PscI ACATGT 1 cut(s) 21
Psp6I CCWGG 2 cut(s) 83, 312
PspFI CCCAGC 1 cut(s) 271
PspGI CCWGG 2 cut(s) 83, 312
PspN4I GGNNCC 2 cut(s) 263, 353
PvuII CAGCTG 1 cut(s) 388
RsaI GTAC 2 cut(s) 226, 495
RsaNI GTAC 2 cut(s) 225, 494
SaqAI TTAA 3 cut(s) 119, 250, 434
SatI GCNGC 3 cut(s) 323, 389, 392
Sau3AI GATC 2 cut(s) 197, 344
ScaI AGTACT 1 cut(s) 226
ScrFI CCNGG 2 cut(s) 85, 314
SmlI CTYRAG 1 cut(s) 31
SmoI CTYRAG 1 cut(s) 31
SnaBI TACGTA 1 cut(s) 497
Sse9I AATT 3 cut(s) 155, 246, 483
SsiI CCGC 2 cut(s) 349, 391
StyD4I CCNGG 2 cut(s) 83, 312
TaaI ACNGT 1 cut(s) 229
TaiI ACGT 1 cut(s) 499
TaqI TCGA 2 cut(s) 370, 444
TasI AATT 3 cut(s) 155, 246, 483
TatI WGTACW 1 cut(s) 224
TauI GCSGC 1 cut(s) 394
TfiI GAWTC 1 cut(s) 276
Tru1I TTAA 3 cut(s) 119, 250, 434
Tru9I TTAA 3 cut(s) 119, 250, 434
TscAI CASTG 2 cut(s) 402, 465
TseFI GTSAC 2 cut(s) 172, 187
TseI GCWGC 2 cut(s) 322, 388
Tsp45I GTSAC 2 cut(s) 172, 187
TspDTI ATGAA 1 cut(s) 369
TspGWI ACGGA 1 cut(s) 39
TspRI CASTG 2 cut(s) 402, 465
Van91I CCANNNNNTGG 1 cut(s) 271
XagI CCTNNNNNAGG 1 cut(s) 121
XapI RAATTY 2 cut(s) 246, 483
XceI RCATGY 1 cut(s) 25
XmnI GAANNNNTTC 1 cut(s) 376
ZrmI AGTACT 1 cut(s) 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.