RLG00000021140

2-alkenal reductase (NADP( )-dependent)-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0003278
Physical Location & Seq
Reverse (-)
72271892 .. 72280463
8572 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021140

Sequence Viewer

Length: 840 bp
ATGACTGGCCGAGAAGACGGCCTCTACTCTCCCCAGTACAACCTCAATGAGGTAGTTTCAGCATTTGGGGTTGGAAGGGTACTTCGGTCAAGGGATAGTAACTACGGGGAAGGTGACATAGTCATCAGTCCCTTCATCCCTGTTGCTGAATATTTTGTGCTCCCATCCCAAGCCTTAACAAGAAAGATTGATGCAGATGCTGGGATTCCATTGCCTGAATACATCAGTACGCTAGGAGTACCCGGGTTTGCAGCGTGGGTGGGGATTCAGGTGGTGGCAGAGCCAATAAAACAAGGGTCGAATGTGTTCATTTCAGCCGCAGCTGGGGCGGTTGGGATGTATGCAGGGCAGTTGGCCAAGCTCAAAGGCTGTAGGGTTGTAAGGCTCATAAAGGAGGAATTTGGATATGATGATGCATTCAACTATCACAGGGAGACAGATTATGATGCTGCTCTAAGCAAGTATTTCCCTAATGGAATTGATGTATACCTTGATAATGTTGGTGGTGAAATGCTTGAGGCTGTTCTCAACCACGTCAACCAACACGCAAAGATACCTCTTTGTGGCATGATATCTGGCTACAACAAGGTTTGGACTGAGAGAGAGGGAGTGAGGAATCTGCTAAATTTGATAGGAAAAGATGTGAAGATGCAAGGTTTCATGGTGGCTTCATACTTGGATCGTTTTGGGGATTTTGCAAAGGAGATGGAGAGCCACCTAAAGGAACGAAAAATTAATTCTAAGCTTAAAATCTATAATGGAATTGACAAGTTCTTGGAGAGCCTACGGTCACTCTTCACCAGCTCTAATATCGGAAAAGTGGTAATCCAAGTCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N_2 ADH_zinc_N

Protein Analysis

280

Amino Acids

30.88

Weight (kDa)

6.45

Isoelectric Point (pI)

25.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_zinc_N PF00107 110 - 232 5.6e-14 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 486
AciI CCGC 2 cut(s) 318, 329
AclWI GGATC 1 cut(s) 687
AcoI YGGCCR 2 cut(s) 7, 354
AcsI RAATTY 2 cut(s) 398, 625
AfaI GTAC 4 cut(s) 38, 81, 229, 240
AfiI CCNNNNNNNGG 4 cut(s) 49, 324, 563, 721
AgsI TTSAA 1 cut(s) 421
AjiI CACGTC 1 cut(s) 535
AluBI AGCT 4 cut(s) 323, 361, 745, 804
AluI AGCT 4 cut(s) 323, 361, 745, 804
Alw21I GWGCWC 1 cut(s) 162
Alw26I GTCTC 1 cut(s) 428
AlwI GGATC 1 cut(s) 687
AlwNI CAGNNNCTG 1 cut(s) 200
Ama87I CYCGRG 1 cut(s) 242
AoxI GGCC 3 cut(s) 7, 19, 354
ApeKI GCWGC 3 cut(s) 251, 320, 449
ApoI RAATTY 2 cut(s) 398, 625
AseI ATTAAT 1 cut(s) 735
Asp700I GAANNNNTTC 1 cut(s) 305
AsuC2I CCSGG 2 cut(s) 243, 244
AsuHPI GGTGA 3 cut(s) 125, 518, 790
AvaI CYCGRG 1 cut(s) 242
BaeI ACNNNNGTAYC 2 cut(s) 71, 104
BalI TGGCCA 1 cut(s) 356
BbsI GAAGAC 1 cut(s) 21
Bbv12I GWGCWC 1 cut(s) 162
BbvI GCAGC 3 cut(s) 263, 332, 436
BccI CCATC 2 cut(s) 172, 700
BceAI ACGGC 1 cut(s) 34
BcnI CCSGG 2 cut(s) 243, 244
BcoDI GTCTC 1 cut(s) 428
BfaI CTAG 1 cut(s) 233
BfmI CTRYAG 1 cut(s) 370
BisI GCNGC 4 cut(s) 252, 318, 321, 450
BlsI GCNGC 4 cut(s) 253, 319, 322, 451
Bme1390I CCNGG 2 cut(s) 243, 244
BmeT110I CYCGRG 1 cut(s) 242
BmgBI CACGTC 1 cut(s) 535
BmrFI CCNGG 2 cut(s) 243, 244
BmrI ACTGGG 1 cut(s) 28
BmsI GCATC 5 cut(s) 181, 187, 403, 436, 639
BmuI ACTGGG 1 cut(s) 28
BpiI GAAGAC 1 cut(s) 21
BpuEI CTTGAG 1 cut(s) 536
BpuMI CCSGG 2 cut(s) 243, 244
BsaJI CCNNGG 1 cut(s) 242
Bsc4I CCNNNNNNNGG 4 cut(s) 49, 324, 563, 721
Bse1I ACTGG 2 cut(s) 10, 34
Bse3DI GCAATG 1 cut(s) 209
BseDI CCNNGG 1 cut(s) 242
BseGI GGATG 3 cut(s) 135, 164, 342
BseLI CCNNNNNNNGG 4 cut(s) 49, 324, 563, 721
BseMI GCAATG 1 cut(s) 209
BseMII CTCAG 1 cut(s) 588
BseNI ACTGG 2 cut(s) 10, 34
BseXI GCAGC 3 cut(s) 263, 332, 436
BseYI CCCAGC 2 cut(s) 200, 323
BshFI GGCC 3 cut(s) 9, 21, 356
BsiHKAI GWGCWC 1 cut(s) 162
BsiHKCI CYCGRG 1 cut(s) 242
BsiSI CCGG 1 cut(s) 243
BslFI GGGAC 1 cut(s) 114
BslI CCNNNNNNNGG 4 cut(s) 49, 324, 563, 721
BsmAI GTCTC 1 cut(s) 428
BsmFI GGGAC 1 cut(s) 114
BsmI GAATGC 1 cut(s) 416
BsnI GGCC 3 cut(s) 9, 21, 356
BsoBI CYCGRG 1 cut(s) 242
Bsp1286I GDGCHC 1 cut(s) 162
Bsp143I GATC 1 cut(s) 679
BspACI CCGC 2 cut(s) 318, 329
BspANI GGCC 3 cut(s) 9, 21, 356
BspCNI CTCAG 1 cut(s) 589
BspPI GGATC 1 cut(s) 687
BsrDI GCAATG 1 cut(s) 209
BsrI ACTGG 2 cut(s) 10, 34
BssECI CCNNGG 1 cut(s) 242
BssMI GATC 1 cut(s) 679
BssNAI GTATAC 1 cut(s) 487
Bst1107I GTATAC 1 cut(s) 487
Bst4CI ACNGT 1 cut(s) 789
Bst6I CTCTTC 1 cut(s) 800
BstDEI CTNAG 3 cut(s) 455, 597, 741
BstENI CCTNNNNNAGG 1 cut(s) 47
BstF5I GGATG 3 cut(s) 135, 164, 342
BstKTI GATC 1 cut(s) 682
BstMAI GTCTC 1 cut(s) 428
BstMBI GATC 1 cut(s) 679
BstMWI GCNNNNNNNGC 1 cut(s) 326
BstSCI CCNGG 2 cut(s) 241, 242
BstSFI CTRYAG 1 cut(s) 370
BstV1I GCAGC 3 cut(s) 263, 332, 436
BstV2I GAAGAC 1 cut(s) 21
BstZ17I GTATAC 1 cut(s) 487
BsuRI GGCC 3 cut(s) 9, 21, 356
BtrI CACGTC 1 cut(s) 535
BtsCI GGATG 3 cut(s) 135, 164, 342
CaiI CAGNNNCTG 1 cut(s) 200
Cfr9I CCCGGG 1 cut(s) 242
Csp6I GTAC 4 cut(s) 37, 80, 228, 239
CviAII CATG 2 cut(s) 568, 661
CviQI GTAC 4 cut(s) 37, 80, 228, 239
DdeI CTNAG 3 cut(s) 455, 597, 741
DpnI GATC 1 cut(s) 681
DpnII GATC 1 cut(s) 679
EaeI YGGCCR 2 cut(s) 7, 354
Eam1104I CTCTTC 1 cut(s) 800
EarI CTCTTC 1 cut(s) 800
Eco32I GATATC 1 cut(s) 573
Eco88I CYCGRG 1 cut(s) 242
EcoNI CCTNNNNNAGG 1 cut(s) 47
EcoRV GATATC 1 cut(s) 573
EcoT22I ATGCAT 1 cut(s) 418
FaeI CATG 2 cut(s) 571, 664
FaqI GGGAC 1 cut(s) 114
FatI CATG 2 cut(s) 567, 660
FblI GTMKAC 1 cut(s) 486
Fnu4HI GCNGC 4 cut(s) 252, 318, 321, 450
FokI GGATG 3 cut(s) 122, 151, 349
Fsp4HI GCNGC 4 cut(s) 252, 318, 321, 450
FspBI CTAG 1 cut(s) 233
GluI GCNGC 4 cut(s) 252, 318, 321, 450
GsaI CCCAGC 2 cut(s) 204, 327
HaeIII GGCC 3 cut(s) 9, 21, 356
HapII CCGG 1 cut(s) 243
Hin1II CATG 2 cut(s) 571, 664
HincII GTYRAC 1 cut(s) 538
HindII GTYRAC 1 cut(s) 538
HindIII AAGCTT 1 cut(s) 743
HinfI GANTC 3 cut(s) 205, 265, 616
HpaII CCGG 1 cut(s) 243
HphI GGTGA 3 cut(s) 125, 518, 790
Hpy166II GTNNAC 2 cut(s) 487, 538
Hpy188I TCNGA 1 cut(s) 815
Hpy8I GTNNAC 2 cut(s) 487, 538
HpyAV CCTTC 3 cut(s) 69, 104, 142
HpyCH4III ACNGT 1 cut(s) 789
HpyCH4IV ACGT 1 cut(s) 534
HpyCH4V TGCA 6 cut(s) 194, 251, 344, 416, 652, 698
HpyF10VI GCNNNNNNNGC 1 cut(s) 326
HpyF3I CTNAG 3 cut(s) 455, 597, 741
HpySE526I ACGT 1 cut(s) 534
Hsp92II CATG 2 cut(s) 571, 664
Kzo9I GATC 1 cut(s) 679
LmnI GCTCC 1 cut(s) 165
Lsp1109I GCAGC 3 cut(s) 263, 332, 436
LweI GCATC 5 cut(s) 181, 187, 403, 436, 639
MaeI CTAG 1 cut(s) 233
MaeII ACGT 1 cut(s) 534
MaeIII GTNAC 3 cut(s) 98, 113, 789
MalI GATC 1 cut(s) 681
MboI GATC 1 cut(s) 679
MboII GAAGA 3 cut(s) 26, 658, 787
MhlI GDGCHC 1 cut(s) 162
MlsI TGGCCA 1 cut(s) 356
MluCI AATT 6 cut(s) 398, 477, 625, 732, 736, 762
MluNI TGGCCA 1 cut(s) 356
MmeI TCCRAC 1 cut(s) 52
MnlI CCTC 8 cut(s) 32, 43, 53, 388, 511, 567, 598, 606
Mox20I TGGCCA 1 cut(s) 356
Mph1103I ATGCAT 1 cut(s) 418
MroXI GAANNNNTTC 1 cut(s) 305
MscI TGGCCA 1 cut(s) 356
MseI TTAA 3 cut(s) 176, 735, 747
Msp20I TGGCCA 1 cut(s) 356
MspA1I CMGCKG 1 cut(s) 323
MspI CCGG 1 cut(s) 243
MspR9I CCNGG 2 cut(s) 243, 244
Mva1269I GAATGC 1 cut(s) 416
MwoI GCNNNNNNNGC 1 cut(s) 326
NciI CCSGG 2 cut(s) 243, 244
NdeII GATC 1 cut(s) 679
NlaIII CATG 2 cut(s) 571, 664
NmeAIII GCCGAG 1 cut(s) 35
NmuCI GTSAC 2 cut(s) 113, 789
NsiI ATGCAT 1 cut(s) 418
PctI GAATGC 1 cut(s) 416
PdmI GAANNNNTTC 1 cut(s) 305
PfeI GAWTC 3 cut(s) 205, 265, 616
PflFI GACNNNGTC 1 cut(s) 119
PkrI GCNGC 4 cut(s) 253, 319, 322, 451
PshBI ATTAAT 1 cut(s) 735
PspFI CCCAGC 2 cut(s) 200, 323
PstNI CAGNNNCTG 1 cut(s) 200
PsyI GACNNNGTC 1 cut(s) 119
PvuII CAGCTG 1 cut(s) 323
RsaI GTAC 4 cut(s) 38, 81, 229, 240
RsaNI GTAC 4 cut(s) 37, 80, 228, 239
SaqAI TTAA 3 cut(s) 176, 735, 747
SatI GCNGC 4 cut(s) 252, 318, 321, 450
Sau3AI GATC 1 cut(s) 679
ScrFI CCNGG 2 cut(s) 243, 244
SduI GDGCHC 1 cut(s) 162
SfaNI GCATC 5 cut(s) 181, 187, 403, 436, 639
SfcI CTRYAG 1 cut(s) 370
SmaI CCCGGG 1 cut(s) 244
SmlI CTYRAG 1 cut(s) 515
SmoI CTYRAG 1 cut(s) 515
Sse9I AATT 6 cut(s) 398, 477, 625, 732, 736, 762
SsiI CCGC 2 cut(s) 318, 329
SspI AATATT 1 cut(s) 152
SspMI CTAG 1 cut(s) 233
StyD4I CCNGG 2 cut(s) 241, 242
TaaI ACNGT 1 cut(s) 789
TaiI ACGT 1 cut(s) 537
TaqI TCGA 1 cut(s) 299
TaqII GACCGA 1 cut(s) 75
TasI AATT 6 cut(s) 398, 477, 625, 732, 736, 762
TatI WGTACW 1 cut(s) 36
TauI GCSGC 1 cut(s) 320
TfiI GAWTC 3 cut(s) 205, 265, 616
Tru1I TTAA 3 cut(s) 176, 735, 747
Tru9I TTAA 3 cut(s) 176, 735, 747
TseFI GTSAC 2 cut(s) 113, 789
TseI GCWGC 3 cut(s) 251, 320, 449
Tsp45I GTSAC 2 cut(s) 113, 789
TspDTI ATGAA 4 cut(s) 124, 298, 649, 660
TspMI CCCGGG 1 cut(s) 242
Tth111I GACNNNGTC 1 cut(s) 119
VspI ATTAAT 1 cut(s) 735
XagI CCTNNNNNAGG 1 cut(s) 47
XapI RAATTY 2 cut(s) 398, 625
XmaI CCCGGG 1 cut(s) 242
XmiI GTMKAC 1 cut(s) 486
XmnI GAANNNNTTC 1 cut(s) 305
XspI CTAG 1 cut(s) 233
Zsp2I ATGCAT 1 cut(s) 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.