MD09G1109800.v1.1

2-alkenal reductase (NADP( )-dependent)-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0003278
Physical Location & Seq
Forward (+)
8139238 .. 8141861
2624 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1109800.v1.1.491

Sequence Viewer

Length: 534 bp
ATGTATGCTGGACAGTTGGCCAAGCTAAGAGGATGCAGGGTCATCGGAAGCACCGGATCAGATGAGAAGGTGAGGCTAATCAAGGAGGAATTTGGATATGATGATGCTTTTAACTACCACACAGAGACGGATTTTGATGCTGCTTTAAGCAAGTATTTCCCTAATGGAATTGATGTCTACCTTGACAATGTTGGCGGTGAAATGCTTGAGGCTGCTCTTAACCATGTCAACAAGCATGCAAAGATCCCTCTTTGTGGCATGATATCTGGCTACAATAAGGTTTGGACTGAGAGAGAGGGGGTGAGGAATCTGCTGAATTTAATCGGAAAGGAGGTGAATATGCAAGGGTTCTTGGTGGGTTCATACCTGCATCGTTATGGAGATTTTGCAACCGAGATGGAGAGCCACGTAAAGCAAGGCAAGATTGGTTATTCTAAGCTCAAAATCTTCCATGGAATTGAAATATTATTGGAGAGCCTAGGATCCCTTTTCACCAGCTCTAATGTTGGGAAAGTAGTAATCCAAGCCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N_2 ADH_zinc_N

Protein Analysis

178

Amino Acids

19.7

Weight (kDa)

6.59

Isoelectric Point (pI)

18.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_zinc_N PF00107 2 - 126 1.5e-22 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 375
AccI GTMKAC 1 cut(s) 177
AciI CCGC 1 cut(s) 195
AclWI GGATC 4 cut(s) 64, 238, 477, 490
AcoI YGGCCR 1 cut(s) 18
AcsI RAATTY 2 cut(s) 89, 316
AfiI CCNNNNNNNGG 1 cut(s) 254
AgsI TTSAA 1 cut(s) 461
AluBI AGCT 3 cut(s) 25, 439, 498
AluI AGCT 3 cut(s) 25, 439, 498
Alw26I GTCTC 1 cut(s) 119
AlwI GGATC 4 cut(s) 64, 238, 477, 490
AoxI GGCC 1 cut(s) 18
ApeKI GCWGC 2 cut(s) 140, 212
ApoI RAATTY 2 cut(s) 89, 316
AspA2I CCTAGG 1 cut(s) 478
AsuHPI GGTGA 5 cut(s) 82, 209, 313, 346, 484
AvrII CCTAGG 1 cut(s) 478
BalI TGGCCA 1 cut(s) 20
BamHI GGATCC 1 cut(s) 482
BbvI GCAGC 2 cut(s) 127, 199
BccI CCATC 1 cut(s) 391
BcgI CGANNNNNNTGC 2 cut(s) 25, 59
BcoDI GTCTC 1 cut(s) 119
BfaI CTAG 1 cut(s) 479
BfuAI ACCTGC 1 cut(s) 375
BisI GCNGC 2 cut(s) 141, 213
BlnI CCTAGG 1 cut(s) 478
BlsI GCNGC 2 cut(s) 142, 214
BmiI GGNNCC 1 cut(s) 484
BmsI GCATC 4 cut(s) 23, 94, 127, 379
BpuEI CTTGAG 1 cut(s) 227
BsaAI YACGTR 1 cut(s) 409
BsaJI CCNNGG 2 cut(s) 451, 478
BsaWI WCCGGW 1 cut(s) 53
Bsc4I CCNNNNNNNGG 1 cut(s) 254
BseDI CCNNGG 2 cut(s) 451, 478
BseGI GGATG 1 cut(s) 38
BseLI CCNNNNNNNGG 1 cut(s) 254
BseMII CTCAG 1 cut(s) 279
BseXI GCAGC 2 cut(s) 127, 199
BshFI GGCC 1 cut(s) 20
BsiSI CCGG 1 cut(s) 54
BslI CCNNNNNNNGG 1 cut(s) 254
BsmAI GTCTC 1 cut(s) 119
BsmBI CGTCTC 1 cut(s) 119
BsnI GGCC 1 cut(s) 20
Bsp143I GATC 3 cut(s) 56, 243, 482
Bsp19I CCATGG 1 cut(s) 451
BspACI CCGC 1 cut(s) 195
BspANI GGCC 1 cut(s) 20
BspCNI CTCAG 1 cut(s) 280
BspLI GGNNCC 1 cut(s) 484
BspMI ACCTGC 1 cut(s) 375
BspPI GGATC 4 cut(s) 64, 238, 477, 490
BssECI CCNNGG 2 cut(s) 451, 478
BssMI GATC 3 cut(s) 56, 243, 482
BssT1I CCWWGG 2 cut(s) 451, 478
Bst4CI ACNGT 1 cut(s) 15
BstBAI YACGTR 1 cut(s) 409
BstC8I GCNNGC 1 cut(s) 237
BstDEI CTNAG 3 cut(s) 26, 288, 435
BstDSI CCRYGG 1 cut(s) 451
BstF5I GGATG 1 cut(s) 38
BstKTI GATC 3 cut(s) 59, 246, 485
BstMAI GTCTC 1 cut(s) 119
BstMBI GATC 3 cut(s) 56, 243, 482
BstNSI RCATGY 1 cut(s) 239
BstV1I GCAGC 2 cut(s) 127, 199
BstX2I RGATCY 2 cut(s) 243, 482
BstYI RGATCY 2 cut(s) 243, 482
BsuRI GGCC 1 cut(s) 20
BtgI CCRYGG 1 cut(s) 451
BtsCI GGATG 1 cut(s) 38
BveI ACCTGC 1 cut(s) 375
Cac8I GCNNGC 1 cut(s) 237
CviAII CATG 4 cut(s) 224, 236, 259, 452
DdeI CTNAG 3 cut(s) 26, 288, 435
DpnI GATC 3 cut(s) 58, 245, 484
DpnII GATC 3 cut(s) 56, 243, 482
EaeI YGGCCR 1 cut(s) 18
Eco130I CCWWGG 2 cut(s) 451, 478
Eco32I GATATC 1 cut(s) 264
EcoRV GATATC 1 cut(s) 264
EcoT14I CCWWGG 2 cut(s) 451, 478
ErhI CCWWGG 2 cut(s) 451, 478
Esp3I CGTCTC 1 cut(s) 119
FaeI CATG 4 cut(s) 227, 239, 262, 455
FaiI YATR 9 cut(s) 6, 99, 225, 237, 260, 341, 364, 378, 453
FatI CATG 4 cut(s) 223, 235, 258, 451
FblI GTMKAC 1 cut(s) 177
Fnu4HI GCNGC 2 cut(s) 141, 213
FokI GGATG 1 cut(s) 45
Fsp4HI GCNGC 2 cut(s) 141, 213
FspBI CTAG 1 cut(s) 479
GluI GCNGC 2 cut(s) 141, 213
HaeIII GGCC 1 cut(s) 20
HapII CCGG 1 cut(s) 54
Hin1II CATG 4 cut(s) 227, 239, 262, 455
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HinfI GANTC 1 cut(s) 307
HpaII CCGG 1 cut(s) 54
HphI GGTGA 5 cut(s) 82, 209, 313, 346, 484
Hpy166II GTNNAC 2 cut(s) 178, 229
Hpy188I TCNGA 3 cut(s) 47, 61, 326
Hpy8I GTNNAC 2 cut(s) 178, 229
HpyAV CCTTC 1 cut(s) 61
HpyCH4III ACNGT 1 cut(s) 15
HpyCH4IV ACGT 1 cut(s) 408
HpyCH4V TGCA 5 cut(s) 36, 239, 343, 370, 389
HpyF3I CTNAG 3 cut(s) 26, 288, 435
HpySE526I ACGT 1 cut(s) 408
Hsp92II CATG 4 cut(s) 227, 239, 262, 455
Kzo9I GATC 3 cut(s) 56, 243, 482
LpnPI CCDG 5 cut(s) 22, 67, 252, 380, 508
Lsp1109I GCAGC 2 cut(s) 127, 199
LweI GCATC 4 cut(s) 23, 94, 127, 379
MaeI CTAG 1 cut(s) 479
MaeII ACGT 1 cut(s) 408
MalI GATC 3 cut(s) 58, 245, 484
MboI GATC 3 cut(s) 56, 243, 482
MboII GAAGA 1 cut(s) 439
MflI RGATCY 2 cut(s) 243, 482
MlsI TGGCCA 1 cut(s) 20
MluCI AATT 4 cut(s) 89, 168, 316, 456
MluNI TGGCCA 1 cut(s) 20
MnlI CCTC 8 cut(s) 23, 66, 79, 202, 258, 289, 297, 325
Mox20I TGGCCA 1 cut(s) 20
MscI TGGCCA 1 cut(s) 20
MseI TTAA 4 cut(s) 111, 146, 219, 320
MslI CAYNNNNRTG 1 cut(s) 375
Msp20I TGGCCA 1 cut(s) 20
MspI CCGG 1 cut(s) 54
NcoI CCATGG 1 cut(s) 451
NdeII GATC 3 cut(s) 56, 243, 482
NlaIII CATG 4 cut(s) 227, 239, 262, 455
NlaIV GGNNCC 1 cut(s) 484
NspI RCATGY 1 cut(s) 239
PaeI GCATGC 1 cut(s) 239
PfeI GAWTC 1 cut(s) 307
PkrI GCNGC 2 cut(s) 142, 214
Ppu21I YACGTR 1 cut(s) 409
PspN4I GGNNCC 1 cut(s) 484
PsuI RGATCY 2 cut(s) 243, 482
RseI CAYNNNNRTG 1 cut(s) 375
SaqAI TTAA 4 cut(s) 111, 146, 219, 320
SatI GCNGC 2 cut(s) 141, 213
Sau3AI GATC 3 cut(s) 56, 243, 482
SetI ASST 9 cut(s) 27, 72, 183, 282, 336, 369, 411, 441, 500
SfaNI GCATC 4 cut(s) 23, 94, 127, 379
SmiMI CAYNNNNRTG 1 cut(s) 375
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
SphI GCATGC 1 cut(s) 239
Sse9I AATT 4 cut(s) 89, 168, 316, 456
SsiI CCGC 1 cut(s) 195
SspI AATATT 1 cut(s) 465
SspMI CTAG 1 cut(s) 479
StyI CCWWGG 2 cut(s) 451, 478
TaaI ACNGT 1 cut(s) 15
TaiI ACGT 1 cut(s) 411
TasI AATT 4 cut(s) 89, 168, 316, 456
TfiI GAWTC 1 cut(s) 307
Tru1I TTAA 4 cut(s) 111, 146, 219, 320
Tru9I TTAA 4 cut(s) 111, 146, 219, 320
TseI GCWGC 2 cut(s) 140, 212
TspDTI ATGAA 1 cut(s) 351
TspGWI ACGGA 1 cut(s) 143
XapI RAATTY 2 cut(s) 89, 316
XceI RCATGY 1 cut(s) 239
XmaJI CCTAGG 1 cut(s) 478
XmiI GTMKAC 1 cut(s) 177
XspI CTAG 1 cut(s) 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.