MD09G1109600.v1.1

2-alkenal reductase (NADP( )-dependent)-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0003278
Physical Location & Seq
Forward (+)
8115015 .. 8120883
5869 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1109600.v1.1.491

Sequence Viewer

Length: 810 bp
ATGAAAACTGATCGCACGATATACAATGAACAGACACGATCACAAAAAAGGAAGAGACCGGTATTTGTAAGAAGAAACAAACTCCAGTACGAGGTGAGGAAGCTAGGCGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGATAGAAGAAGTTGAAAGCAGAGAATGGTACTTGGCTGCTTATGCAGCTGAAGGTGTTCCAACTTCTGATCATCTCAAACTCCGTACGGTCACACTCTCACTATCCCCTGATTCCATTCCCGAACAACATGTCATTCTTGAGACCCTCTTCATTTCCGTCGAGCCATATTTACGCAGCAGAATTACTGGCCGGGAAGATGGGCTATACAACCCCCAGTTCAACCTTGACGAGGTAATTGCAGGATTTGGGGTTGGAAGGGTAATTCGGTCAAAAGATAGTAACCATGTTGAAGGTGACATAGTCGTCAGTCCATTCACACATTTTGCTGAATATTGTGTGGTACCATCCCAGTTCGTAGCAAGAAGGATCGATCCAAATAATGGGATCCCCTTGCCTGAATATATCAGTTCGTTAGGGTTGCCGGGGTTTGCAGCATGGGTGGGAATTGAGTTGCTGGCAGACCCAAAACCAGGATCCAATGTGTTCATTTCAGCTGCTGCTGGGGCTGTTGCAATGTATGCTGGACAGTTGGCCAAGCTCAGAGGATGCACGGTCATCGGAAGCACCGGATCAGATGAGAAGGTAAGACCTAGGTACCAGGAATATGACCCAAGCTACAAGGCATTCCCCTACCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N_2 ADH_zinc_N

Protein Analysis

270

Amino Acids

30.77

Weight (kDa)

6.94

Isoelectric Point (pI)

48.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N_2 PF16884 64 - 172 1.7e-15 N-terminal domain of oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 473
Acc65I GGTACC 2 cut(s) 511, 765
AccB1I GGYRCC 2 cut(s) 511, 765
AccB7I CCANNNNNTGG 1 cut(s) 551
AclWI GGATC 7 cut(s) 536, 545, 550, 563, 639, 652, 748
AcoI YGGCCR 2 cut(s) 358, 702
AcuI CTGAAG 1 cut(s) 240
AfaI GTAC 5 cut(s) 89, 200, 256, 513, 767
AfiI CCNNNNNNNGG 4 cut(s) 91, 400, 551, 641
AflIII ACRYGT 1 cut(s) 298
AgeI ACCGGT 1 cut(s) 58
AgsI TTSAA 3 cut(s) 185, 391, 461
AjnI CCWGG 2 cut(s) 640, 768
AluBI AGCT 5 cut(s) 103, 218, 665, 709, 786
AluI AGCT 5 cut(s) 103, 218, 665, 709, 786
Alw26I GTCTC 2 cut(s) 49, 305
AlwI GGATC 7 cut(s) 536, 545, 550, 563, 639, 652, 748
AlwNI CAGNNNCTG 1 cut(s) 668
AoxI GGCC 2 cut(s) 358, 702
ApeKI GCWGC 6 cut(s) 206, 215, 345, 602, 665, 668
ArsI GACNNNNNNTTYG 2 cut(s) 285, 317
AsiGI ACCGGT 1 cut(s) 58
Asp700I GAANNNNTTC 1 cut(s) 225
Asp718I GGTACC 2 cut(s) 511, 765
AspA2I CCTAGG 1 cut(s) 761
AsuC2I CCSGG 2 cut(s) 362, 594
AsuHPI GGTGA 2 cut(s) 106, 476
AvrII CCTAGG 1 cut(s) 761
BaeI ACNNNNGTAYC 2 cut(s) 749, 782
BalI TGGCCA 1 cut(s) 704
BamHI GGATCC 2 cut(s) 555, 644
BanI GGYRCC 2 cut(s) 511, 765
BbvI GCAGC 6 cut(s) 193, 227, 357, 614, 652, 655
BccI CCATC 2 cut(s) 362, 523
BcgI CGANNNNNNTGC 4 cut(s) 389, 423, 709, 743
BciT130I CCWGG 2 cut(s) 642, 770
BclI TGATCA 1 cut(s) 238
BcnI CCSGG 2 cut(s) 362, 594
BcoDI GTCTC 2 cut(s) 49, 305
BfaI CTAG 2 cut(s) 104, 762
BisI GCNGC 6 cut(s) 207, 216, 346, 603, 666, 669
BlnI CCTAGG 1 cut(s) 761
BlsI GCNGC 6 cut(s) 208, 217, 347, 604, 667, 670
Bme1390I CCNGG 4 cut(s) 362, 594, 642, 770
BmiI GGNNCC 4 cut(s) 513, 557, 646, 767
BmrFI CCNGG 4 cut(s) 362, 594, 642, 770
BmrI ACTGGG 2 cut(s) 379, 514
BmsI GCATC 1 cut(s) 707
BmuI ACTGGG 2 cut(s) 379, 514
BpmI CTGGAG 1 cut(s) 68
BpuEI CTTGAG 1 cut(s) 329
BpuMI CCSGG 2 cut(s) 362, 594
Bsa29I ATCGAT 1 cut(s) 540
BsaI GGTCTC 2 cut(s) 49, 305
BsaJI CCNNGG 2 cut(s) 593, 761
BsaWI WCCGGW 2 cut(s) 58, 737
Bsc4I CCNNNNNNNGG 4 cut(s) 91, 400, 551, 641
Bse118I RCCGGY 1 cut(s) 58
Bse1I ACTGG 4 cut(s) 85, 361, 385, 520
Bse3DI GCAATG 1 cut(s) 690
BseBI CCWGG 2 cut(s) 642, 770
BseCI ATCGAT 1 cut(s) 540
BseDI CCNNGG 2 cut(s) 593, 761
BseGI GGATG 2 cut(s) 515, 722
BseLI CCNNNNNNNGG 4 cut(s) 91, 400, 551, 641
BseMI GCAATG 1 cut(s) 690
BseMII CTCAG 1 cut(s) 724
BseNI ACTGG 4 cut(s) 85, 361, 385, 520
BseXI GCAGC 6 cut(s) 193, 227, 357, 614, 652, 655
BseYI CCCAGC 1 cut(s) 671
BshFI GGCC 2 cut(s) 360, 704
BshNI GGYRCC 2 cut(s) 511, 765
BshTI ACCGGT 1 cut(s) 58
BshVI ATCGAT 1 cut(s) 540
BsiSI CCGG 4 cut(s) 59, 361, 593, 738
BsiWI CGTACG 1 cut(s) 254
BslI CCNNNNNNNGG 4 cut(s) 91, 400, 551, 641
BsmAI GTCTC 2 cut(s) 49, 305
BsmI GAATGC 1 cut(s) 794
BsnI GGCC 2 cut(s) 360, 704
Bso31I GGTCTC 2 cut(s) 49, 305
Bsp143I GATC 8 cut(s) 10, 38, 238, 537, 541, 555, 644, 740
BspANI GGCC 2 cut(s) 360, 704
BspCNI CTCAG 1 cut(s) 723
BspDI ATCGAT 1 cut(s) 540
BspLI GGNNCC 4 cut(s) 513, 557, 646, 767
BspPI GGATC 7 cut(s) 536, 545, 550, 563, 639, 652, 748
BspT107I GGYRCC 2 cut(s) 511, 765
BspTNI GGTCTC 2 cut(s) 49, 305
BsrDI GCAATG 1 cut(s) 690
BsrFI RCCGGY 1 cut(s) 58
BsrI ACTGG 4 cut(s) 85, 361, 385, 520
BssAI RCCGGY 1 cut(s) 58
BssECI CCNNGG 2 cut(s) 593, 761
BssMI GATC 8 cut(s) 10, 38, 238, 537, 541, 555, 644, 740
BssT1I CCWWGG 1 cut(s) 761
Bst2UI CCWGG 2 cut(s) 642, 770
Bst4CI ACNGT 3 cut(s) 259, 699, 724
Bst6I CTCTTC 2 cut(s) 47, 323
BstAPI GCANNNNNTGC 1 cut(s) 689
BstC8I GCNNGC 1 cut(s) 627
BstDEI CTNAG 1 cut(s) 710
BstENI CCTNNNNNAGG 1 cut(s) 398
BstF5I GGATG 2 cut(s) 515, 722
BstKTI GATC 8 cut(s) 13, 41, 241, 540, 544, 558, 647, 743
BstMAI GTCTC 2 cut(s) 49, 305
BstMBI GATC 8 cut(s) 10, 38, 238, 537, 541, 555, 644, 740
BstMWI GCNNNNNNNGC 4 cut(s) 212, 215, 674, 689
BstNI CCWGG 2 cut(s) 642, 770
BstNSI RCATGY 1 cut(s) 302
BstSCI CCNGG 4 cut(s) 360, 592, 640, 768
BstV1I GCAGC 6 cut(s) 193, 227, 357, 614, 652, 655
BstX2I RGATCY 2 cut(s) 555, 644
BstYI RGATCY 2 cut(s) 555, 644
Bsu15I ATCGAT 1 cut(s) 540
BsuRI GGCC 2 cut(s) 360, 704
BsuTUI ATCGAT 1 cut(s) 540
BtsCI GGATG 2 cut(s) 515, 722
Cac8I GCNNGC 1 cut(s) 627
CaiI CAGNNNCTG 1 cut(s) 668
Cfr10I RCCGGY 1 cut(s) 58
ClaI ATCGAT 1 cut(s) 540
Csp6I GTAC 5 cut(s) 88, 199, 255, 512, 766
CspAI ACCGGT 1 cut(s) 58
CviAII CATG 3 cut(s) 299, 455, 606
CviQI GTAC 5 cut(s) 88, 199, 255, 512, 766
DdeI CTNAG 1 cut(s) 710
DpnI GATC 8 cut(s) 12, 40, 240, 539, 543, 557, 646, 742
DpnII GATC 8 cut(s) 10, 38, 238, 537, 541, 555, 644, 740
DrdI GACNNNNNNGTC 1 cut(s) 473
DseDI GACNNNNNNGTC 1 cut(s) 473
EaeI YGGCCR 2 cut(s) 358, 702
Eam1104I CTCTTC 2 cut(s) 47, 323
EarI CTCTTC 2 cut(s) 47, 323
Eco130I CCWWGG 1 cut(s) 761
Eco31I GGTCTC 2 cut(s) 49, 305
Eco57I CTGAAG 1 cut(s) 240
EcoNI CCTNNNNNAGG 1 cut(s) 398
EcoRII CCWGG 2 cut(s) 640, 768
EcoT14I CCWWGG 1 cut(s) 761
ErhI CCWWGG 1 cut(s) 761
FaeI CATG 3 cut(s) 302, 458, 609
FatI CATG 3 cut(s) 298, 454, 605
FbaI TGATCA 1 cut(s) 238
Fnu4HI GCNGC 6 cut(s) 207, 216, 346, 603, 666, 669
FokI GGATG 2 cut(s) 502, 729
Fsp4HI GCNGC 6 cut(s) 207, 216, 346, 603, 666, 669
FspBI CTAG 2 cut(s) 104, 762
GluI GCNGC 6 cut(s) 207, 216, 346, 603, 666, 669
GsaI CCCAGC 1 cut(s) 675
GsuI CTGGAG 1 cut(s) 68
HaeIII GGCC 2 cut(s) 360, 704
HapII CCGG 4 cut(s) 59, 361, 593, 738
Hin1II CATG 3 cut(s) 302, 458, 609
HinfI GANTC 1 cut(s) 281
HpaII CCGG 4 cut(s) 59, 361, 593, 738
HphI GGTGA 2 cut(s) 106, 476
Hpy188I TCNGA 4 cut(s) 238, 713, 731, 745
Hpy188III TCNNGA 2 cut(s) 290, 308
Hpy99I CGWCG 1 cut(s) 332
HpyAV CCTTC 5 cut(s) 215, 420, 455, 528, 745
HpyCH4III ACNGT 3 cut(s) 259, 699, 724
HpyCH4V TGCA 5 cut(s) 215, 410, 602, 683, 720
HpyF10VI GCNNNNNNNGC 4 cut(s) 212, 215, 674, 689
HpyF3I CTNAG 1 cut(s) 710
Hsp92II CATG 3 cut(s) 302, 458, 609
KpnI GGTACC 2 cut(s) 515, 769
Ksp22I TGATCA 1 cut(s) 238
Kzo9I GATC 8 cut(s) 10, 38, 238, 537, 541, 555, 644, 740
Lsp1109I GCAGC 6 cut(s) 193, 227, 357, 614, 652, 655
LweI GCATC 1 cut(s) 707
MaeI CTAG 2 cut(s) 104, 762
MaeIII GTNAC 3 cut(s) 259, 449, 464
MalI GATC 8 cut(s) 12, 40, 240, 539, 543, 557, 646, 742
MboI GATC 8 cut(s) 10, 38, 238, 537, 541, 555, 644, 740
MboII GAAGA 5 cut(s) 64, 84, 188, 310, 377
MflI RGATCY 2 cut(s) 555, 644
MlsI TGGCCA 1 cut(s) 704
MluCI AATT 4 cut(s) 351, 405, 432, 615
MluNI TGGCCA 1 cut(s) 704
MmeI TCCRAC 2 cut(s) 254, 403
MnlI CCTC 5 cut(s) 85, 90, 326, 394, 707
Mox20I TGGCCA 1 cut(s) 704
MroXI GAANNNNTTC 1 cut(s) 225
MscI TGGCCA 1 cut(s) 704
Msp20I TGGCCA 1 cut(s) 704
MspA1I CMGCKG 2 cut(s) 218, 665
MspI CCGG 4 cut(s) 59, 361, 593, 738
MspR9I CCNGG 4 cut(s) 362, 594, 642, 770
Mva1269I GAATGC 1 cut(s) 794
MvaI CCWGG 2 cut(s) 642, 770
MwoI GCNNNNNNNGC 4 cut(s) 212, 215, 674, 689
NciI CCSGG 2 cut(s) 362, 594
NdeII GATC 8 cut(s) 10, 38, 238, 537, 541, 555, 644, 740
NlaIII CATG 3 cut(s) 302, 458, 609
NlaIV GGNNCC 4 cut(s) 513, 557, 646, 767
NmuCI GTSAC 2 cut(s) 259, 464
NspI RCATGY 1 cut(s) 302
PciI ACATGT 1 cut(s) 298
PctI GAATGC 1 cut(s) 794
PdmI GAANNNNTTC 1 cut(s) 225
PfeI GAWTC 1 cut(s) 281
Pfl23II CGTACG 1 cut(s) 254
PflFI GACNNNGTC 1 cut(s) 470
PflMI CCANNNNNTGG 1 cut(s) 551
PinAI ACCGGT 1 cut(s) 58
PkrI GCNGC 6 cut(s) 208, 217, 347, 604, 667, 670
PscI ACATGT 1 cut(s) 298
Psp6I CCWGG 2 cut(s) 640, 768
PspFI CCCAGC 1 cut(s) 671
PspGI CCWGG 2 cut(s) 640, 768
PspLI CGTACG 1 cut(s) 254
PspN4I GGNNCC 4 cut(s) 513, 557, 646, 767
PstNI CAGNNNCTG 1 cut(s) 668
PsuI RGATCY 2 cut(s) 555, 644
PsyI GACNNNGTC 1 cut(s) 470
PvuII CAGCTG 2 cut(s) 218, 665
RsaI GTAC 5 cut(s) 89, 200, 256, 513, 767
RsaNI GTAC 5 cut(s) 88, 199, 255, 512, 766
SatI GCNGC 6 cut(s) 207, 216, 346, 603, 666, 669
Sau3AI GATC 8 cut(s) 10, 38, 238, 537, 541, 555, 644, 740
ScrFI CCNGG 4 cut(s) 362, 594, 642, 770
SfaNI GCATC 1 cut(s) 707
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
Sse9I AATT 4 cut(s) 351, 405, 432, 615
SspI AATATT 1 cut(s) 503
SspMI CTAG 2 cut(s) 104, 762
StyD4I CCNGG 4 cut(s) 360, 592, 640, 768
StyI CCWWGG 1 cut(s) 761
TaaI ACNGT 3 cut(s) 259, 699, 724
TaqI TCGA 2 cut(s) 330, 540
TaqII GACCGA 1 cut(s) 426
TasI AATT 4 cut(s) 351, 405, 432, 615
TfiI GAWTC 1 cut(s) 281
TseFI GTSAC 2 cut(s) 259, 464
TseI GCWGC 6 cut(s) 206, 215, 345, 602, 665, 668
Tsp45I GTSAC 2 cut(s) 259, 464
TspDTI ATGAA 4 cut(s) 17, 42, 310, 646
TspGWI ACGGA 2 cut(s) 242, 316
Tth111I GACNNNGTC 1 cut(s) 470
Van91I CCANNNNNTGG 1 cut(s) 551
XagI CCTNNNNNAGG 1 cut(s) 398
XceI RCATGY 1 cut(s) 302
XmaJI CCTAGG 1 cut(s) 761
XmnI GAANNNNTTC 1 cut(s) 225
XspI CTAG 2 cut(s) 104, 762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.