Rorug02G0474200

2-alkenal reductase (NADP( )-dependent)-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003278
Physical Location & Seq
Reverse (-)
60541642 .. 60543267
1626 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0474200.1

Sequence Viewer

Length: 864 bp
ATGGGTGCTGTGATGATGAACAAAGCATCTTCTTCTTTGTTCATATGGGCTTTGGTTGCTGGTATGCTCTCCCACAACCTGGTCATCCCTGTCATGTCCACCACCCTTGAGGACCAGAAGAACTACTACACTCCGGACCCCGGCGCTGGAACTCCCCCTTCAGGCCACGGTGGAAGCCCTCCCGGAGGTTCACATACAAGTCCTCCTTCCCATACACCATCTCATCATAACCCTCCCTCTCATGGAAGCTACAATCCTCCCTCTCATGGAAGCTACAACCCTACACCATCACCACCTTCCAACTGTGGTACCCCACCATCAAGACCATCTACTCCTTCAACCCCATCAACTCCAACCTACGGAACTCCACCCTCAGGCGGTAGCCCCCCTATCATTGTTACTCCTCCAACACCAGTCACCGTAAGCCCCCCAATCCCATTCATCGGAACTCCACCAATCGACCCCGGTACTCCAGGTGTTCCTACACCTCCCTATCTTCCTGATCCCAACTCCCCTCCCTTCACATGCACCTACTGGAGGAACCACCCCACAGTGATATGGGGTCTTGTGGGCTGGTTTGGAACTTTGGGCCATGCTTTTGGTCTGACTAGCCTTCCAGGGTTCGGATCAAGCACCATGAATTTGCAGCAAGCACTTTCCAACACTCGCGCCGATGGACTTGGGGAACTCTACAGAGAAGGAACTGCTTCCTTGCTCAACTCTATGGTGGACAACAGGTTCCATTTTACTACCAAGCAAGTCAGGGATGGTTTCGTCAGGGCACTGGGTTCGAACAAGGCAGCAACAAATCAAGCTCGTCTCTTCAGGCTGGCCAACGAGGGAAAACTCAAGCCCAGAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N_2 ADH_zinc_N

Protein Analysis

287

Amino Acids

30.07

Weight (kDa)

9.36

Isoelectric Point (pI)

43.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 308
AccB1I GGYRCC 1 cut(s) 308
AccB7I CCANNNNNTGG 1 cut(s) 79
AccII CGCG 1 cut(s) 669
AccIII TCCGGA 1 cut(s) 133
AciI CCGC 1 cut(s) 378
AclWI GGATC 2 cut(s) 497, 634
AcoI YGGCCR 1 cut(s) 831
AcsI RAATTY 1 cut(s) 640
AcuI CTGAAG 2 cut(s) 144, 808
AfaI GTAC 2 cut(s) 310, 469
AgsI TTSAA 1 cut(s) 339
AjnI CCWGG 3 cut(s) 78, 472, 616
AloI GAACNNNNNNTCC 2 cut(s) 722, 754
AluBI AGCT 4 cut(s) 249, 273, 815, 860
AluI AGCT 4 cut(s) 249, 273, 815, 860
Alw26I GTCTC 1 cut(s) 824
AlwI GGATC 2 cut(s) 497, 634
Aor13HI TCCGGA 1 cut(s) 133
AoxI GGCC 3 cut(s) 163, 589, 831
ApeKI GCWGC 2 cut(s) 646, 800
ApoI RAATTY 1 cut(s) 640
Asp700I GAANNNNTTC 1 cut(s) 706
Asp718I GGTACC 1 cut(s) 308
AspLEI GCGC 2 cut(s) 146, 671
AspS9I GGNCC 3 cut(s) 112, 136, 589
AsuC2I CCSGG 3 cut(s) 141, 183, 465
AsuHPI GGTGA 2 cut(s) 282, 409
AsuII TTCGAA 1 cut(s) 791
AvaII GGWCC 2 cut(s) 112, 136
AxyI CCTNAGG 1 cut(s) 373
BaeGI GKGCMC 1 cut(s) 784
BaeI ACNNNNGTAYC 2 cut(s) 292, 325
BalI TGGCCA 1 cut(s) 833
BanI GGYRCC 1 cut(s) 308
BarI GAAGNNNNNNTAC 2 cut(s) 110, 142
BbvI GCAGC 2 cut(s) 658, 812
BccI CCATC 7 cut(s) 226, 295, 325, 334, 352, 668, 761
BcgI CGANNNNNNTGC 2 cut(s) 771, 805
BciT130I CCWGG 3 cut(s) 80, 474, 618
BcnI CCSGG 3 cut(s) 141, 183, 465
BcoDI GTCTC 1 cut(s) 824
BfaI CTAG 1 cut(s) 609
BfmI CTRYAG 1 cut(s) 691
BfoI RGCGCY 1 cut(s) 147
BisI GCNGC 2 cut(s) 647, 801
BlsI GCNGC 2 cut(s) 648, 802
Bme1390I CCNGG 6 cut(s) 80, 141, 183, 465, 474, 618
Bme18I GGWCC 2 cut(s) 112, 136
BmgT120I GGNCC 3 cut(s) 112, 136, 589
BmiI GGNNCC 4 cut(s) 138, 310, 542, 740
BmrFI CCNGG 6 cut(s) 80, 141, 183, 465, 474, 618
BmrI ACTGGG 1 cut(s) 794
BmsI GCATC 1 cut(s) 35
BmuI ACTGGG 1 cut(s) 794
BpmI CTGGAG 2 cut(s) 456, 556
Bpu14I TTCGAA 1 cut(s) 791
BpuEI CTTGAG 2 cut(s) 128, 833
BpuMI CCSGG 3 cut(s) 141, 183, 465
BsaJI CCNNGG 4 cut(s) 139, 166, 463, 617
BsaWI WCCGGW 1 cut(s) 133
BsaXI ACNNNNNCTCC 2 cut(s) 187, 217
Bse1I ACTGG 3 cut(s) 413, 539, 789
Bse21I CCTNAGG 1 cut(s) 373
BseAI TCCGGA 1 cut(s) 133
BseBI CCWGG 3 cut(s) 80, 474, 618
BseDI CCNNGG 4 cut(s) 139, 166, 463, 617
BseGI GGATG 2 cut(s) 84, 772
BseMII CTCAG 1 cut(s) 387
BseNI ACTGG 3 cut(s) 413, 539, 789
BseRI GAGGAG 1 cut(s) 393
BseSI GKGCMC 1 cut(s) 784
BseXI GCAGC 2 cut(s) 658, 812
Bsh1236I CGCG 1 cut(s) 669
BshFI GGCC 3 cut(s) 165, 591, 833
BshNI GGYRCC 1 cut(s) 308
BsiSI CCGG 4 cut(s) 134, 141, 183, 465
BsmAI GTCTC 1 cut(s) 824
BsmBI CGTCTC 1 cut(s) 824
BsnI GGCC 3 cut(s) 165, 591, 833
Bsp119I TTCGAA 1 cut(s) 791
Bsp1286I GDGCHC 1 cut(s) 784
Bsp13I TCCGGA 1 cut(s) 133
Bsp143I GATC 2 cut(s) 502, 626
BspACI CCGC 1 cut(s) 378
BspANI GGCC 3 cut(s) 165, 591, 833
BspCNI CTCAG 1 cut(s) 386
BspEI TCCGGA 1 cut(s) 133
BspFNI CGCG 1 cut(s) 669
BspLI GGNNCC 4 cut(s) 138, 310, 542, 740
BspPI GGATC 2 cut(s) 497, 634
BspT104I TTCGAA 1 cut(s) 791
BspT107I GGYRCC 1 cut(s) 308
BsrI ACTGG 3 cut(s) 413, 539, 789
BssECI CCNNGG 4 cut(s) 139, 166, 463, 617
BssMI GATC 2 cut(s) 502, 626
Bst2UI CCWGG 3 cut(s) 80, 474, 618
Bst4CI ACNGT 4 cut(s) 170, 305, 421, 553
Bst6I CTCTTC 1 cut(s) 827
BstBI TTCGAA 1 cut(s) 791
BstC8I GCNNGC 2 cut(s) 651, 831
BstDEI CTNAG 1 cut(s) 373
BstDSI CCRYGG 1 cut(s) 166
BstENI CCTNNNNNAGG 2 cut(s) 183, 535
BstF5I GGATG 2 cut(s) 84, 772
BstFNI CGCG 1 cut(s) 669
BstH2I RGCGCY 1 cut(s) 147
BstHHI GCGC 2 cut(s) 146, 671
BstKTI GATC 2 cut(s) 505, 629
BstMAI GTCTC 1 cut(s) 824
BstMBI GATC 2 cut(s) 502, 626
BstMWI GCNNNNNNNGC 1 cut(s) 56
BstNI CCWGG 3 cut(s) 80, 474, 618
BstNSI RCATGY 1 cut(s) 528
BstSCI CCNGG 6 cut(s) 78, 139, 181, 463, 472, 616
BstSFI CTRYAG 1 cut(s) 691
BstSLI GKGCMC 1 cut(s) 784
BstUI CGCG 1 cut(s) 669
BstV1I GCAGC 2 cut(s) 658, 812
BstXI CCANNNNNNTGG 1 cut(s) 599
Bsu36I CCTNAGG 1 cut(s) 373
BsuRI GGCC 3 cut(s) 165, 591, 833
BtgI CCRYGG 1 cut(s) 166
BtsCI GGATG 2 cut(s) 84, 772
BtsIMutI CAGTG 2 cut(s) 558, 782
Cac8I GCNNGC 2 cut(s) 651, 831
CfoI GCGC 2 cut(s) 146, 671
Cfr13I GGNCC 3 cut(s) 112, 136, 589
CsiI ACCWGGT 1 cut(s) 78
Csp6I GTAC 2 cut(s) 309, 468
CspCI CAANNNNNGTGG 2 cut(s) 88, 123
CviAII CATG 6 cut(s) 94, 242, 266, 525, 593, 637
CviQI GTAC 2 cut(s) 309, 468
DdeI CTNAG 1 cut(s) 373
DpnI GATC 2 cut(s) 504, 628
DpnII GATC 2 cut(s) 502, 626
EaeI YGGCCR 1 cut(s) 831
Eam1104I CTCTTC 1 cut(s) 827
EarI CTCTTC 1 cut(s) 827
Eco47I GGWCC 2 cut(s) 112, 136
Eco57I CTGAAG 2 cut(s) 144, 808
Eco81I CCTNAGG 1 cut(s) 373
EcoNI CCTNNNNNAGG 2 cut(s) 183, 535
EcoRII CCWGG 3 cut(s) 78, 472, 616
Esp3I CGTCTC 1 cut(s) 824
FaeI CATG 6 cut(s) 97, 245, 269, 528, 596, 640
FalI AAGNNNNNCTT 2 cut(s) 190, 222
FatI CATG 6 cut(s) 93, 241, 265, 524, 592, 636
FauNDI CATATG 1 cut(s) 44
Fnu4HI GCNGC 2 cut(s) 647, 801
FokI GGATG 2 cut(s) 71, 779
Fsp4HI GCNGC 2 cut(s) 647, 801
FspBI CTAG 1 cut(s) 609
GlaI GCGC 2 cut(s) 145, 670
GluI GCNGC 2 cut(s) 647, 801
GsuI CTGGAG 2 cut(s) 456, 556
HaeII RGCGCY 1 cut(s) 147
HaeIII GGCC 3 cut(s) 165, 591, 833
HapII CCGG 4 cut(s) 134, 141, 183, 465
HhaI GCGC 2 cut(s) 146, 671
Hin1II CATG 6 cut(s) 97, 245, 269, 528, 596, 640
Hin6I GCGC 2 cut(s) 144, 669
HinP1I GCGC 2 cut(s) 144, 669
HpaII CCGG 4 cut(s) 134, 141, 183, 465
HphI GGTGA 2 cut(s) 282, 409
Hpy166II GTNNAC 3 cut(s) 99, 191, 730
Hpy188I TCNGA 3 cut(s) 446, 606, 626
Hpy188III TCNNGA 3 cut(s) 134, 321, 500
Hpy8I GTNNAC 3 cut(s) 99, 191, 730
HpyAV CCTTC 7 cut(s) 168, 216, 306, 345, 529, 623, 692
HpyCH4III ACNGT 4 cut(s) 170, 305, 421, 553
HpyCH4V TGCA 2 cut(s) 528, 646
HpyF10VI GCNNNNNNNGC 1 cut(s) 56
HpyF3I CTNAG 1 cut(s) 373
Hsp92II CATG 6 cut(s) 97, 245, 269, 528, 596, 640
HspAI GCGC 2 cut(s) 144, 669
Kpn2I TCCGGA 1 cut(s) 133
KpnI GGTACC 1 cut(s) 312
Kzo9I GATC 2 cut(s) 502, 626
Lsp1109I GCAGC 2 cut(s) 658, 812
LweI GCATC 1 cut(s) 35
MabI ACCWGGT 1 cut(s) 78
MaeI CTAG 1 cut(s) 609
MaeIII GTNAC 2 cut(s) 397, 415
MalI GATC 2 cut(s) 504, 628
MboI GATC 2 cut(s) 502, 626
MboII GAAGA 5 cut(s) 21, 24, 130, 488, 814
MhlI GDGCHC 1 cut(s) 784
MlsI TGGCCA 1 cut(s) 833
MluCI AATT 1 cut(s) 640
MluNI TGGCCA 1 cut(s) 833
MmeI TCCRAC 4 cut(s) 324, 377, 431, 684
Mox20I TGGCCA 1 cut(s) 833
MroI TCCGGA 1 cut(s) 133
MroXI GAANNNNTTC 1 cut(s) 706
MscI TGGCCA 1 cut(s) 833
Msp20I TGGCCA 1 cut(s) 833
MspI CCGG 4 cut(s) 134, 141, 183, 465
MspR9I CCNGG 6 cut(s) 80, 141, 183, 465, 474, 618
MvaI CCWGG 3 cut(s) 80, 474, 618
MvnI CGCG 1 cut(s) 669
MwoI GCNNNNNNNGC 1 cut(s) 56
NciI CCSGG 3 cut(s) 141, 183, 465
NdeI CATATG 1 cut(s) 44
NdeII GATC 2 cut(s) 502, 626
NlaIII CATG 6 cut(s) 97, 245, 269, 528, 596, 640
NlaIV GGNNCC 4 cut(s) 138, 310, 542, 740
NmuCI GTSAC 1 cut(s) 415
NspI RCATGY 1 cut(s) 528
NspV TTCGAA 1 cut(s) 791
PdmI GAANNNNTTC 1 cut(s) 706
PflMI CCANNNNNTGG 1 cut(s) 79
PfoI TCCNGGA 1 cut(s) 181
PkrI GCNGC 2 cut(s) 648, 802
Psp6I CCWGG 3 cut(s) 78, 472, 616
PspGI CCWGG 3 cut(s) 78, 472, 616
PspN4I GGNNCC 4 cut(s) 138, 310, 542, 740
PspPI GGNCC 3 cut(s) 112, 136, 589
RsaI GTAC 2 cut(s) 310, 469
RsaNI GTAC 2 cut(s) 309, 468
SatI GCNGC 2 cut(s) 647, 801
Sau3AI GATC 2 cut(s) 502, 626
Sau96I GGNCC 3 cut(s) 112, 136, 589
ScrFI CCNGG 6 cut(s) 80, 141, 183, 465, 474, 618
SduI GDGCHC 1 cut(s) 784
SexAI ACCWGGT 1 cut(s) 78
SfaNI GCATC 1 cut(s) 35
SfcI CTRYAG 1 cut(s) 691
SfuI TTCGAA 1 cut(s) 791
SinI GGWCC 2 cut(s) 112, 136
SmlI CTYRAG 2 cut(s) 107, 848
SmoI CTYRAG 2 cut(s) 107, 848
Sse9I AATT 1 cut(s) 640
SsiI CCGC 1 cut(s) 378
SspMI CTAG 1 cut(s) 609
StyD4I CCNGG 6 cut(s) 78, 139, 181, 463, 472, 616
TaaI ACNGT 4 cut(s) 170, 305, 421, 553
TaqI TCGA 2 cut(s) 459, 791
TasI AATT 1 cut(s) 640
TscAI CASTG 2 cut(s) 558, 789
TseFI GTSAC 1 cut(s) 415
TseI GCWGC 2 cut(s) 646, 800
Tsp45I GTSAC 1 cut(s) 415
TspDTI ATGAA 4 cut(s) 31, 32, 430, 653
TspGWI ACGGA 1 cut(s) 375
TspRI CASTG 2 cut(s) 558, 789
Van91I CCANNNNNTGG 1 cut(s) 79
VpaK11BI GGWCC 2 cut(s) 112, 136
XagI CCTNNNNNAGG 2 cut(s) 183, 535
XapI RAATTY 1 cut(s) 640
XceI RCATGY 1 cut(s) 528
XmnI GAANNNNTTC 1 cut(s) 706
XspI CTAG 1 cut(s) 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.