Rroxscaffold_2G00090760

2-alkenal reductase (NADP( )-dependent)-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003278
Physical Location & Seq
Forward (+)
12476610 .. 12491828
15219 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00090760.1

Sequence Viewer

Length: 543 bp
ATGAGTGCTACAGTAATCGAAAGCAGAGAATGGTACTTGGCTGCTTATGCTCCTGAAGGTATTCCAACTTCAGATCATCTGAAAATTCGCTGCGTCTCACTGGCCCTTGATGCAATCCCTGAAGAACATGTCGTCCTTGAGACGCTTTTCGTATCCGTTGAACCATACCTGCGTGGCAGAATGACCGGCCTGGAAGAAGGGCTATACTTTGCGCAGTTCAACCTTAACGAGGTAGTTTCAGCATTTGGTGTTGGAAGAGTAATTATGTCAAAGGATAGTGACTATGGTGAAGGTGACATAGTGATCTGTCCTTCCACATCTTTTGCCGAGTACTGTGTGGTATCATCCCAGAATTTAAGAAAGGTGGAACCAAATGCTGGGATTCCATTGCCTGAATATCTCGGTTTGTTAGGGATACCTGGGTTGGCAGCGTGGGTGGGGAATAGAGGTGATCGCGGAGCCAATAAAAGCAGGGTCGAATGTGTTCATCTCAGCCGCGGCCAGTGCCGTTGGGATGTATGCAGGGCAGTTGGCCAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N_2 ADH_zinc_N

Protein Analysis

180

Amino Acids

19.67

Weight (kDa)

5.1

Isoelectric Point (pI)

34.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N_2 PF16884 9 - 122 7.7e-18 N-terminal domain of oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 213
Acc36I ACCTGC 1 cut(s) 177
AccB7I CCANNNNNTGG 1 cut(s) 377
AccII CGCG 2 cut(s) 456, 498
AciI CCGC 3 cut(s) 456, 496, 498
AcoI YGGCCR 2 cut(s) 499, 532
AcsI RAATTY 2 cut(s) 84, 352
AcuI CTGAAG 3 cut(s) 54, 75, 141
AfaI GTAC 2 cut(s) 35, 332
AfiI CCNNNNNNNGG 2 cut(s) 229, 377
AflIII ACRYGT 1 cut(s) 127
AgsI TTSAA 2 cut(s) 161, 220
AjnI CCWGG 2 cut(s) 189, 418
AloI GAACNNNNNNTCC 2 cut(s) 117, 149
AluBI AGCT 1 cut(s) 539
AluI AGCT 1 cut(s) 539
Alw26I GTCTC 2 cut(s) 100, 134
AoxI GGCC 4 cut(s) 102, 187, 499, 532
ApeKI GCWGC 3 cut(s) 41, 90, 428
ApoI RAATTY 2 cut(s) 84, 352
Asp700I GAANNNNTTC 2 cut(s) 60, 483
AspLEI GCGC 1 cut(s) 214
AspS9I GGNCC 1 cut(s) 103
AsuHPI GGTGA 3 cut(s) 299, 305, 461
BaeI ACNNNNGTAYC 2 cut(s) 407, 440
BalI TGGCCA 1 cut(s) 534
BarI GAAGNNNNNNTAC 2 cut(s) 52, 84
BbvI GCAGC 3 cut(s) 28, 77, 440
BceAI ACGGC 1 cut(s) 492
BciT130I CCWGG 2 cut(s) 191, 420
BciVI GTATCC 2 cut(s) 163, 408
BcoDI GTCTC 2 cut(s) 100, 134
BfmI CTRYAG 1 cut(s) 9
BfuAI ACCTGC 1 cut(s) 177
BfuI GTATCC 2 cut(s) 163, 408
BisI GCNGC 5 cut(s) 42, 91, 429, 496, 499
BlsI GCNGC 5 cut(s) 43, 92, 430, 497, 500
BmcAI AGTACT 1 cut(s) 332
Bme1390I CCNGG 2 cut(s) 191, 420
BmgT120I GGNCC 1 cut(s) 103
BmiI GGNNCC 2 cut(s) 369, 460
BmrFI CCNGG 2 cut(s) 191, 420
BmsI GCATC 1 cut(s) 100
BpuEI CTTGAG 1 cut(s) 158
BsaJI CCNNGG 2 cut(s) 419, 496
Bsc4I CCNNNNNNNGG 2 cut(s) 229, 377
Bse118I RCCGGY 1 cut(s) 185
Bse1I ACTGG 2 cut(s) 105, 502
Bse3DI GCAATG 1 cut(s) 386
BseBI CCWGG 2 cut(s) 191, 420
BseDI CCNNGG 2 cut(s) 419, 496
BseGI GGATG 2 cut(s) 344, 520
BseLI CCNNNNNNNGG 2 cut(s) 229, 377
BseMI GCAATG 1 cut(s) 386
BseMII CTCAG 1 cut(s) 505
BseNI ACTGG 2 cut(s) 105, 502
BseXI GCAGC 3 cut(s) 28, 77, 440
BseYI CCCAGC 1 cut(s) 377
Bsh1236I CGCG 2 cut(s) 456, 498
BshFI GGCC 4 cut(s) 104, 189, 501, 534
BsiSI CCGG 1 cut(s) 186
BslI CCNNNNNNNGG 2 cut(s) 229, 377
BsmAI GTCTC 2 cut(s) 100, 134
BsmBI CGTCTC 2 cut(s) 100, 134
BsnI GGCC 4 cut(s) 104, 189, 501, 534
Bsp143I GATC 3 cut(s) 73, 303, 451
BspACI CCGC 3 cut(s) 456, 496, 498
BspANI GGCC 4 cut(s) 104, 189, 501, 534
BspCNI CTCAG 1 cut(s) 504
BspFNI CGCG 2 cut(s) 456, 498
BspLI GGNNCC 2 cut(s) 369, 460
BspMI ACCTGC 1 cut(s) 177
BsrDI GCAATG 1 cut(s) 386
BsrFI RCCGGY 1 cut(s) 185
BsrI ACTGG 2 cut(s) 105, 502
BssAI RCCGGY 1 cut(s) 185
BssECI CCNNGG 2 cut(s) 419, 496
BssMI GATC 3 cut(s) 73, 303, 451
Bst2UI CCWGG 2 cut(s) 191, 420
Bst4CI ACNGT 2 cut(s) 13, 335
Bst6I CTCTTC 1 cut(s) 250
BstDEI CTNAG 1 cut(s) 491
BstDSI CCRYGG 1 cut(s) 496
BstENI CCTNNNNNAGG 1 cut(s) 227
BstF5I GGATG 2 cut(s) 344, 520
BstFNI CGCG 2 cut(s) 456, 498
BstHHI GCGC 1 cut(s) 214
BstKTI GATC 3 cut(s) 76, 306, 454
BstMAI GTCTC 2 cut(s) 100, 134
BstMBI GATC 3 cut(s) 73, 303, 451
BstMWI GCNNNNNNNGC 3 cut(s) 47, 110, 504
BstNI CCWGG 2 cut(s) 191, 420
BstNSI RCATGY 1 cut(s) 131
BstSCI CCNGG 2 cut(s) 189, 418
BstSFI CTRYAG 1 cut(s) 9
BstUI CGCG 2 cut(s) 456, 498
BstV1I GCAGC 3 cut(s) 28, 77, 440
BsuI GTATCC 2 cut(s) 163, 408
BsuRI GGCC 4 cut(s) 104, 189, 501, 534
BtgI CCRYGG 1 cut(s) 496
BtsCI GGATG 2 cut(s) 344, 520
BtsIMutI CAGTG 2 cut(s) 98, 509
BveI ACCTGC 1 cut(s) 177
CfoI GCGC 1 cut(s) 214
Cfr10I RCCGGY 1 cut(s) 185
Cfr13I GGNCC 1 cut(s) 103
Cfr42I CCGCGG 1 cut(s) 499
CseI GACGC 2 cut(s) 82, 151
Csp6I GTAC 2 cut(s) 34, 331
CspCI CAANNNNNGTGG 2 cut(s) 304, 339
CviAII CATG 1 cut(s) 128
CviJI RGCY 9 cut(s) 41, 104, 189, 202, 461, 495, 501, 534, 539
CviKI_1 RGCY 9 cut(s) 41, 104, 189, 202, 461, 495, 501, 534, 539
CviQI GTAC 2 cut(s) 34, 331
DdeI CTNAG 1 cut(s) 491
DpnI GATC 3 cut(s) 75, 305, 453
DpnII GATC 3 cut(s) 73, 303, 451
EaeI YGGCCR 2 cut(s) 499, 532
Eam1104I CTCTTC 1 cut(s) 250
EarI CTCTTC 1 cut(s) 250
Eco57I CTGAAG 3 cut(s) 54, 75, 141
EcoNI CCTNNNNNAGG 1 cut(s) 227
EcoRII CCWGG 2 cut(s) 189, 418
Esp3I CGTCTC 2 cut(s) 100, 134
FaeI CATG 1 cut(s) 131
FaiI YATR 8 cut(s) 48, 129, 166, 205, 266, 285, 299, 520
FatI CATG 1 cut(s) 127
Fnu4HI GCNGC 5 cut(s) 42, 91, 429, 496, 499
FokI GGATG 2 cut(s) 331, 527
Fsp4HI GCNGC 5 cut(s) 42, 91, 429, 496, 499
FspI TGCGCA 1 cut(s) 213
GlaI GCGC 1 cut(s) 213
GluI GCNGC 5 cut(s) 42, 91, 429, 496, 499
GsaI CCCAGC 1 cut(s) 381
HaeIII GGCC 4 cut(s) 104, 189, 501, 534
HapII CCGG 1 cut(s) 186
HgaI GACGC 2 cut(s) 82, 151
HhaI GCGC 1 cut(s) 214
Hin1II CATG 1 cut(s) 131
Hin6I GCGC 1 cut(s) 212
HinP1I GCGC 1 cut(s) 212
HindIII AAGCTT 1 cut(s) 537
HinfI GANTC 1 cut(s) 382
HpaII CCGG 1 cut(s) 186
HphI GGTGA 3 cut(s) 299, 305, 461
Hpy188I TCNGA 2 cut(s) 73, 81
Hpy188III TCNNGA 1 cut(s) 53
HpyAV CCTTC 4 cut(s) 50, 191, 284, 321
HpyCH4III ACNGT 2 cut(s) 13, 335
HpyCH4V TGCA 2 cut(s) 113, 522
HpyF10VI GCNNNNNNNGC 3 cut(s) 47, 110, 504
HpyF3I CTNAG 1 cut(s) 491
Hsp92II CATG 1 cut(s) 131
HspAI GCGC 1 cut(s) 212
KspI CCGCGG 1 cut(s) 499
Kzo9I GATC 3 cut(s) 73, 303, 451
LmnI GCTCC 2 cut(s) 55, 458
Lsp1109I GCAGC 3 cut(s) 28, 77, 440
LweI GCATC 1 cut(s) 100
MaeIII GTNAC 2 cut(s) 278, 293
MalI GATC 3 cut(s) 75, 305, 453
MboI GATC 3 cut(s) 73, 303, 451
MboII GAAGA 3 cut(s) 134, 206, 267
MlsI TGGCCA 1 cut(s) 534
MluCI AATT 3 cut(s) 84, 261, 352
MluNI TGGCCA 1 cut(s) 534
MmeI TCCRAC 2 cut(s) 89, 232
MnlI CCTC 2 cut(s) 223, 440
Mox20I TGGCCA 1 cut(s) 534
MroXI GAANNNNTTC 2 cut(s) 60, 483
MscI TGGCCA 1 cut(s) 534
MseI TTAA 3 cut(s) 225, 356, 541
Msp20I TGGCCA 1 cut(s) 534
MspA1I CMGCKG 1 cut(s) 498
MspI CCGG 1 cut(s) 186
MspR9I CCNGG 2 cut(s) 191, 420
MvaI CCWGG 2 cut(s) 191, 420
MvnI CGCG 2 cut(s) 456, 498
MwoI GCNNNNNNNGC 3 cut(s) 47, 110, 504
NdeII GATC 3 cut(s) 73, 303, 451
NlaIII CATG 1 cut(s) 131
NlaIV GGNNCC 2 cut(s) 369, 460
NmeAIII GCCGAG 1 cut(s) 352
NmuCI GTSAC 2 cut(s) 278, 293
NsbI TGCGCA 1 cut(s) 213
NspI RCATGY 1 cut(s) 131
PciI ACATGT 1 cut(s) 127
PdmI GAANNNNTTC 2 cut(s) 60, 483
PfeI GAWTC 1 cut(s) 382
PflMI CCANNNNNTGG 1 cut(s) 377
PkrI GCNGC 5 cut(s) 43, 92, 430, 497, 500
PscI ACATGT 1 cut(s) 127
Psp6I CCWGG 2 cut(s) 189, 418
PspFI CCCAGC 1 cut(s) 377
PspGI CCWGG 2 cut(s) 189, 418
PspN4I GGNNCC 2 cut(s) 369, 460
PspPI GGNCC 1 cut(s) 103
RsaI GTAC 2 cut(s) 35, 332
RsaNI GTAC 2 cut(s) 34, 331
SacII CCGCGG 1 cut(s) 499
SaqAI TTAA 3 cut(s) 225, 356, 541
SatI GCNGC 5 cut(s) 42, 91, 429, 496, 499
Sau3AI GATC 3 cut(s) 73, 303, 451
Sau96I GGNCC 1 cut(s) 103
ScaI AGTACT 1 cut(s) 332
ScrFI CCNGG 2 cut(s) 191, 420
SetI ASST 9 cut(s) 61, 171, 225, 234, 295, 366, 421, 451, 541
SfaNI GCATC 1 cut(s) 100
SfcI CTRYAG 1 cut(s) 9
Sfr303I CCGCGG 1 cut(s) 499
SgrBI CCGCGG 1 cut(s) 499
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
Sse9I AATT 3 cut(s) 84, 261, 352
SsiI CCGC 3 cut(s) 456, 496, 498
StyD4I CCNGG 2 cut(s) 189, 418
TaaI ACNGT 2 cut(s) 13, 335
TaqI TCGA 2 cut(s) 18, 477
TasI AATT 3 cut(s) 84, 261, 352
TatI WGTACW 1 cut(s) 330
TauI GCSGC 2 cut(s) 498, 501
TfiI GAWTC 1 cut(s) 382
Tru1I TTAA 3 cut(s) 225, 356, 541
Tru9I TTAA 3 cut(s) 225, 356, 541
TscAI CASTG 2 cut(s) 105, 509
TseFI GTSAC 2 cut(s) 278, 293
TseI GCWGC 3 cut(s) 41, 90, 428
Tsp45I GTSAC 2 cut(s) 278, 293
TspDTI ATGAA 1 cut(s) 476
TspGWI ACGGA 1 cut(s) 145
TspRI CASTG 2 cut(s) 105, 509
Van91I CCANNNNNTGG 1 cut(s) 377
XagI CCTNNNNNAGG 1 cut(s) 227
XapI RAATTY 2 cut(s) 84, 352
XceI RCATGY 1 cut(s) 131
XmnI GAANNNNTTC 2 cut(s) 60, 483
ZrmI AGTACT 1 cut(s) 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.