FvH4_7g00040

EF-hand domain pair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0004510
Physical Location & Seq
Reverse (-)
636555 .. 637397
843 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g00040.t1

Sequence Viewer

Length: 429 bp
ATGGGAGTGGTGATAATCGACGGAACGACGGTGAAGGACTTCGTGAGCGACGAGGTGCAGTTCAAGAAGAGCGTGGACGAGCGTTTTGCTGCTCTAGATCTTAACAACGATGGCGTTCTGTCCCGGTCTGAGTTGCGAAAAGCGTTCGAGTCTATGAGATTGATCGAGACCCACTTCGGCATGGACGTGGCAACCTCGCCTGATGAGCTGACGCAGCTCTACAATTCCATATTCGATAAGTTCGACTGCGACCAGAGCGGGACGGTGGATCTGGACGAGTTCAGGGAGGAGATGAAGAAGATTTTGCTGGCCATCGCCGACGGGCTTGGCTCTTGCCCTATACAGATGGCTCTCGAGGACCAGAGCTTTCTCAAGCAGGCTGCAGATCTCGAGGCTTCCAAGACATCGTCCTCAGTTTCACACACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7

Protein Analysis

143

Amino Acids

15.74

Weight (kDa)

4.39

Isoelectric Point (pI)

36.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 27 - 99 7.1e-12 EF-hand domain pair
EF-hand_1 PF00036 76 - 100 4.3e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 258
AciI CCGC 1 cut(s) 258
AclWI GGATC 1 cut(s) 276
AcoI YGGCCR 1 cut(s) 309
AgsI TTSAA 1 cut(s) 64
AjiI CACGTC 1 cut(s) 187
AluBI AGCT 3 cut(s) 208, 217, 366
AluI AGCT 3 cut(s) 208, 217, 366
Alw26I GTCTC 1 cut(s) 161
AlwI GGATC 1 cut(s) 276
Ama87I CYCGRG 2 cut(s) 353, 389
AoxI GGCC 1 cut(s) 309
ApeKI GCWGC 3 cut(s) 89, 214, 380
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
Asp700I GAANNNNTTC 1 cut(s) 38
AspS9I GGNCC 1 cut(s) 358
AsuC2I CCSGG 1 cut(s) 124
AsuHPI GGTGA 2 cut(s) 22, 43
AvaI CYCGRG 2 cut(s) 353, 389
AvaII GGWCC 1 cut(s) 358
BalI TGGCCA 1 cut(s) 311
BbvI GCAGC 3 cut(s) 76, 226, 367
BccI CCATC 3 cut(s) 104, 320, 340
BcgI CGANNNNNNTGC 2 cut(s) 68, 102
BcnI CCSGG 1 cut(s) 124
BcoDI GTCTC 1 cut(s) 161
BfaI CTAG 2 cut(s) 95, 427
BfmI CTRYAG 1 cut(s) 381
BglII AGATCT 2 cut(s) 97, 385
BisI GCNGC 3 cut(s) 90, 215, 381
BlsI GCNGC 3 cut(s) 91, 216, 382
Bme1390I CCNGG 1 cut(s) 124
Bme18I GGWCC 1 cut(s) 358
BmeT110I CYCGRG 2 cut(s) 353, 389
BmgBI CACGTC 1 cut(s) 187
BmgT120I GGNCC 1 cut(s) 358
BmrFI CCNGG 1 cut(s) 124
BpuEI CTTGAG 1 cut(s) 356
BpuMI CCSGG 1 cut(s) 124
BsaI GGTCTC 1 cut(s) 161
BseMII CTCAG 2 cut(s) 120, 426
BseRI GAGGAG 1 cut(s) 302
BseXI GCAGC 3 cut(s) 76, 226, 367
BsgI GTGCAG 1 cut(s) 77
BshFI GGCC 1 cut(s) 311
BsiHKCI CYCGRG 2 cut(s) 353, 389
BsiSI CCGG 1 cut(s) 124
BslFI GGGAC 2 cut(s) 106, 274
BsmAI GTCTC 1 cut(s) 161
BsmFI GGGAC 2 cut(s) 106, 274
BsnI GGCC 1 cut(s) 311
Bso31I GGTCTC 1 cut(s) 161
BsoBI CYCGRG 2 cut(s) 353, 389
Bsp143I GATC 4 cut(s) 97, 162, 268, 385
BspACI CCGC 1 cut(s) 258
BspANI GGCC 1 cut(s) 311
BspCNI CTCAG 2 cut(s) 121, 425
BspMAI CTGCAG 1 cut(s) 385
BspPI GGATC 1 cut(s) 276
BspQI GCTCTTC 1 cut(s) 62
BspTNI GGTCTC 1 cut(s) 161
BsrBI CCGCTC 1 cut(s) 258
BssMI GATC 4 cut(s) 97, 162, 268, 385
Bst4CI ACNGT 2 cut(s) 31, 265
Bst6I CTCTTC 1 cut(s) 62
BstC8I GCNNGC 2 cut(s) 309, 378
BstDEI CTNAG 2 cut(s) 129, 412
BstKTI GATC 4 cut(s) 100, 165, 271, 388
BstMAI GTCTC 1 cut(s) 161
BstMBI GATC 4 cut(s) 97, 162, 268, 385
BstMWI GCNNNNNNNGC 3 cut(s) 205, 214, 255
BstSCI CCNGG 1 cut(s) 122
BstSFI CTRYAG 1 cut(s) 381
BstV1I GCAGC 3 cut(s) 76, 226, 367
BstX2I RGATCY 3 cut(s) 97, 268, 385
BstYI RGATCY 3 cut(s) 97, 268, 385
BsuRI GGCC 1 cut(s) 311
BtgZI GCGATG 1 cut(s) 298
BtrI CACGTC 1 cut(s) 187
Cac8I GCNNGC 2 cut(s) 309, 378
Cfr13I GGNCC 1 cut(s) 358
CseI GACGC 1 cut(s) 220
CviAII CATG 1 cut(s) 181
CviJI RGCY 9 cut(s) 208, 217, 311, 325, 330, 350, 366, 380, 395
CviKI_1 RGCY 9 cut(s) 208, 217, 311, 325, 330, 350, 366, 380, 395
DdeI CTNAG 2 cut(s) 129, 412
DpnI GATC 4 cut(s) 99, 164, 270, 387
DpnII GATC 4 cut(s) 97, 162, 268, 385
EaeI YGGCCR 1 cut(s) 309
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
Eco31I GGTCTC 1 cut(s) 161
Eco47I GGWCC 1 cut(s) 358
Eco88I CYCGRG 2 cut(s) 353, 389
FaeI CATG 1 cut(s) 184
FaiI YATR 4 cut(s) 155, 182, 230, 341
FaqI GGGAC 2 cut(s) 106, 274
FatI CATG 1 cut(s) 180
FauI CCCGC 1 cut(s) 251
Fnu4HI GCNGC 3 cut(s) 90, 215, 381
Fsp4HI GCNGC 3 cut(s) 90, 215, 381
FspBI CTAG 2 cut(s) 95, 427
GluI GCNGC 3 cut(s) 90, 215, 381
HaeIII GGCC 1 cut(s) 311
HapII CCGG 1 cut(s) 124
HgaI GACGC 1 cut(s) 220
Hin1II CATG 1 cut(s) 184
HinfI GANTC 1 cut(s) 149
HpaII CCGG 1 cut(s) 124
HphI GGTGA 2 cut(s) 22, 43
Hpy166II GTNNAC 1 cut(s) 76
Hpy188I TCNGA 1 cut(s) 130
Hpy188III TCNNGA 7 cut(s) 43, 64, 95, 166, 272, 353, 389
Hpy8I GTNNAC 1 cut(s) 76
Hpy99I CGWCG 4 cut(s) 23, 31, 53, 323
HpyAV CCTTC 1 cut(s) 28
HpyCH4III ACNGT 2 cut(s) 31, 265
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 2 cut(s) 58, 383
HpyF10VI GCNNNNNNNGC 3 cut(s) 205, 214, 255
HpyF3I CTNAG 2 cut(s) 129, 412
HpySE526I ACGT 1 cut(s) 186
Hsp92II CATG 1 cut(s) 184
Kzo9I GATC 4 cut(s) 97, 162, 268, 385
LguI GCTCTTC 1 cut(s) 62
LpnPI CCDG 8 cut(s) 137, 213, 257, 266, 268, 293, 362, 374
Lsp1109I GCAGC 3 cut(s) 76, 226, 367
MaeI CTAG 2 cut(s) 95, 427
MaeII ACGT 1 cut(s) 186
MalI GATC 4 cut(s) 99, 164, 270, 387
MbiI CCGCTC 1 cut(s) 258
MboI GATC 4 cut(s) 97, 162, 268, 385
MboII GAAGA 3 cut(s) 79, 307, 310
MflI RGATCY 3 cut(s) 97, 268, 385
MlsI TGGCCA 1 cut(s) 311
MluCI AATT 1 cut(s) 223
MluNI TGGCCA 1 cut(s) 311
MlyI GAGTC 1 cut(s) 158
MnlI CCTC 6 cut(s) 46, 205, 280, 349, 385, 421
Mox20I TGGCCA 1 cut(s) 311
MroXI GAANNNNTTC 1 cut(s) 38
MscI TGGCCA 1 cut(s) 311
MseI TTAA 1 cut(s) 102
MslI CAYNNNNRTG 1 cut(s) 185
Msp20I TGGCCA 1 cut(s) 311
MspI CCGG 1 cut(s) 124
MspR9I CCNGG 1 cut(s) 124
MwoI GCNNNNNNNGC 3 cut(s) 205, 214, 255
NciI CCSGG 1 cut(s) 124
NdeII GATC 4 cut(s) 97, 162, 268, 385
NlaIII CATG 1 cut(s) 184
PaeR7I CTCGAG 2 cut(s) 353, 389
PciSI GCTCTTC 1 cut(s) 62
PcsI WCGNNNNNNNCGW 3 cut(s) 48, 183, 240
PdmI GAANNNNTTC 1 cut(s) 38
PflFI GACNNNGTC 1 cut(s) 406
PkrI GCNGC 3 cut(s) 91, 216, 382
PleI GAGTC 1 cut(s) 157
PpsI GAGTC 1 cut(s) 157
PspPI GGNCC 1 cut(s) 358
PstI CTGCAG 1 cut(s) 385
PsuI RGATCY 3 cut(s) 97, 268, 385
PsyI GACNNNGTC 1 cut(s) 406
RseI CAYNNNNRTG 1 cut(s) 185
SapI GCTCTTC 1 cut(s) 62
SaqAI TTAA 1 cut(s) 102
SatI GCNGC 3 cut(s) 90, 215, 381
Sau3AI GATC 4 cut(s) 97, 162, 268, 385
Sau96I GGNCC 1 cut(s) 358
SchI GAGTC 1 cut(s) 158
ScrFI CCNGG 1 cut(s) 124
SetI ASST 7 cut(s) 57, 189, 197, 210, 219, 368, 428
SfcI CTRYAG 1 cut(s) 381
Sfr274I CTCGAG 2 cut(s) 353, 389
SinI GGWCC 1 cut(s) 358
SlaI CTCGAG 2 cut(s) 353, 389
SmiMI CAYNNNNRTG 1 cut(s) 185
SmlI CTYRAG 3 cut(s) 353, 371, 389
SmoI CTYRAG 3 cut(s) 353, 371, 389
Sse9I AATT 1 cut(s) 223
SsiI CCGC 1 cut(s) 258
SspMI CTAG 2 cut(s) 95, 427
StyD4I CCNGG 1 cut(s) 122
TaaI ACNGT 2 cut(s) 31, 265
TaiI ACGT 1 cut(s) 189
TaqI TCGA 7 cut(s) 18, 147, 165, 234, 243, 354, 390
TasI AATT 1 cut(s) 223
Tru1I TTAA 1 cut(s) 102
Tru9I TTAA 1 cut(s) 102
TseI GCWGC 3 cut(s) 89, 214, 380
TspDTI ATGAA 1 cut(s) 308
TspGWI ACGGA 1 cut(s) 36
Tth111I GACNNNGTC 1 cut(s) 406
VpaK11BI GGWCC 1 cut(s) 358
XbaI TCTAGA 1 cut(s) 94
XhoI CTCGAG 2 cut(s) 353, 389
XmnI GAANNNNTTC 1 cut(s) 38
XspI CTAG 2 cut(s) 95, 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.