RLG00000013113

EF-hand domain pair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0004510
Physical Location & Seq
Forward (+)
26323912 .. 26324678
767 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013113

Sequence Viewer

Length: 444 bp
ATGTGGGCAGACATTTACGCAGGAAAAGAAGAAAAGAAAAATCGACGGAGCCACGGAGGTAAGATGGGAATGGTGATAATTGACGGAACGACGGTGAAGGACTTCGTGAGCGACGAGGTGCAGTTCAAGAAGAGCGTGGACGAGCGTTTTGCTGCTCTGGATCTGAACAACGATGGTGTTCTGTCCCGGTCGGAGATGCGGAAAGCATTCGAGTCGATGAGATTGATCGAGACCCACTTCGGCATAGAAGTGGCAACCCCGCCGAATCAGCTGACGCAGCTCTACGATTCCATATTCGATAAGTTCGACTGCGACCAGAGAGGGACGGTGGATCTGGACGAGTTCAGGGAGGAGATGAAGAGATTATGGCTGGCCGCTGGTCCTGTCCTGGATTTGTGTGGAGCCGAGACTGAACCCGACTTATCGGTCTCTGTTTGGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7

Protein Analysis

148

Amino Acids

16.71

Weight (kDa)

4.73

Isoelectric Point (pI)

40.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 48 - 120 1.4e-10 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 425
AciI CCGC 3 cut(s) 199, 260, 375
AclWI GGATC 2 cut(s) 168, 339
AcoI YGGCCR 1 cut(s) 372
AgsI TTSAA 1 cut(s) 127
AjnI CCWGG 1 cut(s) 387
AluBI AGCT 2 cut(s) 271, 280
AluI AGCT 2 cut(s) 271, 280
Alw26I GTCTC 3 cut(s) 224, 401, 433
AlwI GGATC 2 cut(s) 168, 339
AoxI GGCC 1 cut(s) 372
ApeKI GCWGC 2 cut(s) 152, 277
ArsI GACNNNNNNTTYG 2 cut(s) 131, 163
Asp700I GAANNNNTTC 2 cut(s) 101, 206
AspS9I GGNCC 2 cut(s) 380, 438
AsuC2I CCSGG 1 cut(s) 187
AsuHPI GGTGA 2 cut(s) 85, 106
AvaII GGWCC 2 cut(s) 380, 438
BbvI GCAGC 2 cut(s) 139, 289
BccI CCATC 2 cut(s) 58, 167
BcgI CGANNNNNNTGC 4 cut(s) 131, 165, 195, 229
BciT130I CCWGG 1 cut(s) 389
BcnI CCSGG 1 cut(s) 187
BcoDI GTCTC 3 cut(s) 224, 401, 433
BisI GCNGC 3 cut(s) 153, 278, 375
BlsI GCNGC 3 cut(s) 154, 279, 376
Bme1390I CCNGG 2 cut(s) 187, 389
Bme18I GGWCC 2 cut(s) 380, 438
BmgT120I GGNCC 2 cut(s) 380, 438
BmiI GGNNCC 2 cut(s) 50, 403
BmrFI CCNGG 2 cut(s) 187, 389
BmsI GCATC 1 cut(s) 186
BpuMI CCSGG 1 cut(s) 187
BsaI GGTCTC 2 cut(s) 224, 433
BsaJI CCNNGG 1 cut(s) 52
BseBI CCWGG 1 cut(s) 389
BseDI CCNNGG 1 cut(s) 52
BseRI GAGGAG 1 cut(s) 365
BseXI GCAGC 2 cut(s) 139, 289
BsgI GTGCAG 1 cut(s) 140
Bsh1285I CGRYCG 1 cut(s) 191
BshFI GGCC 1 cut(s) 374
BsiEI CGRYCG 1 cut(s) 191
BsiSI CCGG 1 cut(s) 187
BslFI GGGAC 2 cut(s) 169, 337
BsmAI GTCTC 3 cut(s) 224, 401, 433
BsmFI GGGAC 2 cut(s) 169, 337
BsmI GAATGC 1 cut(s) 206
BsnI GGCC 1 cut(s) 374
Bso31I GGTCTC 2 cut(s) 224, 433
Bsp143I GATC 3 cut(s) 160, 225, 331
BspACI CCGC 3 cut(s) 199, 260, 375
BspANI GGCC 1 cut(s) 374
BspLI GGNNCC 2 cut(s) 50, 403
BspPI GGATC 2 cut(s) 168, 339
BspQI GCTCTTC 1 cut(s) 125
BspTNI GGTCTC 2 cut(s) 224, 433
BssECI CCNNGG 1 cut(s) 52
BssMI GATC 3 cut(s) 160, 225, 331
Bst2UI CCWGG 1 cut(s) 389
Bst4CI ACNGT 2 cut(s) 94, 328
Bst6I CTCTTC 2 cut(s) 125, 353
BstC8I GCNNGC 1 cut(s) 372
BstDSI CCRYGG 1 cut(s) 52
BstKTI GATC 3 cut(s) 163, 228, 334
BstMAI GTCTC 3 cut(s) 224, 401, 433
BstMBI GATC 3 cut(s) 160, 225, 331
BstMCI CGRYCG 1 cut(s) 191
BstMWI GCNNNNNNNGC 2 cut(s) 268, 277
BstNI CCWGG 1 cut(s) 389
BstSCI CCNGG 2 cut(s) 185, 387
BstV1I GCAGC 2 cut(s) 139, 289
BstX2I RGATCY 2 cut(s) 160, 331
BstYI RGATCY 2 cut(s) 160, 331
BsuRI GGCC 1 cut(s) 374
BtgI CCRYGG 1 cut(s) 52
Cac8I GCNNGC 1 cut(s) 372
Cfr13I GGNCC 2 cut(s) 380, 438
CseI GACGC 1 cut(s) 283
CviJI RGCY 6 cut(s) 51, 271, 280, 370, 374, 404
CviKI_1 RGCY 6 cut(s) 51, 271, 280, 370, 374, 404
DpnI GATC 3 cut(s) 162, 227, 333
DpnII GATC 3 cut(s) 160, 225, 331
DrdI GACNNNNNNGTC 1 cut(s) 425
DseDI GACNNNNNNGTC 1 cut(s) 425
EaeI YGGCCR 1 cut(s) 372
Eam1104I CTCTTC 2 cut(s) 125, 353
EarI CTCTTC 2 cut(s) 125, 353
Eco31I GGTCTC 2 cut(s) 224, 433
Eco47I GGWCC 2 cut(s) 380, 438
EcoRII CCWGG 1 cut(s) 387
FaiI YATR 3 cut(s) 245, 293, 367
FaqI GGGAC 2 cut(s) 169, 337
FauI CCCGC 1 cut(s) 267
Fnu4HI GCNGC 3 cut(s) 153, 278, 375
Fsp4HI GCNGC 3 cut(s) 153, 278, 375
GluI GCNGC 3 cut(s) 153, 278, 375
HaeIII GGCC 1 cut(s) 374
HapII CCGG 1 cut(s) 187
HgaI GACGC 1 cut(s) 283
HinfI GANTC 3 cut(s) 212, 265, 287
HpaII CCGG 1 cut(s) 187
HphI GGTGA 2 cut(s) 85, 106
Hpy166II GTNNAC 1 cut(s) 139
Hpy188I TCNGA 2 cut(s) 165, 193
Hpy188III TCNNGA 6 cut(s) 106, 127, 158, 229, 335, 441
Hpy8I GTNNAC 1 cut(s) 139
Hpy99I CGWCG 3 cut(s) 48, 94, 116
HpyAV CCTTC 1 cut(s) 91
HpyCH4III ACNGT 2 cut(s) 94, 328
HpyCH4V TGCA 1 cut(s) 121
HpyF10VI GCNNNNNNNGC 2 cut(s) 268, 277
Kzo9I GATC 3 cut(s) 160, 225, 331
LguI GCTCTTC 1 cut(s) 125
LmnI GCTCC 2 cut(s) 48, 401
Lsp1109I GCAGC 2 cut(s) 139, 289
LweI GCATC 1 cut(s) 186
MalI GATC 3 cut(s) 162, 227, 333
MboI GATC 3 cut(s) 160, 225, 331
MboII GAAGA 3 cut(s) 41, 142, 370
MflI RGATCY 2 cut(s) 160, 331
MluCI AATT 1 cut(s) 78
MlyI GAGTC 1 cut(s) 221
MmeI TCCRAC 1 cut(s) 171
MnlI CCTC 4 cut(s) 50, 109, 314, 343
MroXI GAANNNNTTC 2 cut(s) 101, 206
MslI CAYNNNNRTG 1 cut(s) 248
MspA1I CMGCKG 2 cut(s) 271, 377
MspI CCGG 1 cut(s) 187
MspR9I CCNGG 2 cut(s) 187, 389
Mva1269I GAATGC 1 cut(s) 206
MvaI CCWGG 1 cut(s) 389
MwoI GCNNNNNNNGC 2 cut(s) 268, 277
NciI CCSGG 1 cut(s) 187
NdeII GATC 3 cut(s) 160, 225, 331
NlaIV GGNNCC 2 cut(s) 50, 403
NmeAIII GCCGAG 1 cut(s) 430
PciSI GCTCTTC 1 cut(s) 125
PcsI WCGNNNNNNNCGW 2 cut(s) 111, 303
PctI GAATGC 1 cut(s) 206
PdmI GAANNNNTTC 2 cut(s) 101, 206
PfeI GAWTC 2 cut(s) 265, 287
PfoI TCCNGGA 1 cut(s) 387
PkrI GCNGC 3 cut(s) 154, 279, 376
PleI GAGTC 1 cut(s) 220
PpsI GAGTC 1 cut(s) 220
Psp6I CCWGG 1 cut(s) 387
PspGI CCWGG 1 cut(s) 387
PspN4I GGNNCC 2 cut(s) 50, 403
PspPI GGNCC 2 cut(s) 380, 438
PsuI RGATCY 2 cut(s) 160, 331
PvuII CAGCTG 1 cut(s) 271
RseI CAYNNNNRTG 1 cut(s) 248
SapI GCTCTTC 1 cut(s) 125
SatI GCNGC 3 cut(s) 153, 278, 375
Sau3AI GATC 3 cut(s) 160, 225, 331
Sau96I GGNCC 2 cut(s) 380, 438
SchI GAGTC 1 cut(s) 221
ScrFI CCNGG 2 cut(s) 187, 389
SetI ASST 4 cut(s) 61, 120, 273, 282
SfaNI GCATC 1 cut(s) 186
SinI GGWCC 2 cut(s) 380, 438
SmiMI CAYNNNNRTG 1 cut(s) 248
Sse9I AATT 1 cut(s) 78
SsiI CCGC 3 cut(s) 199, 260, 375
StyD4I CCNGG 2 cut(s) 185, 387
TaaI ACNGT 2 cut(s) 94, 328
TaqI TCGA 6 cut(s) 43, 210, 215, 228, 297, 306
TaqII GACCGA 1 cut(s) 415
TasI AATT 1 cut(s) 78
TauI GCSGC 1 cut(s) 377
TfiI GAWTC 2 cut(s) 265, 287
TseI GCWGC 2 cut(s) 152, 277
TspDTI ATGAA 1 cut(s) 371
TspGWI ACGGA 3 cut(s) 61, 69, 99
VpaK11BI GGWCC 2 cut(s) 380, 438
XmnI GAANNNNTTC 2 cut(s) 101, 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.