Rw4G006740

EF-hand domain pair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0004510
Physical Location & Seq
Reverse (-)
14126990 .. 14127316
327 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G006740.1

Sequence Viewer

Length: 327 bp
ATGGGAGTGGTGATAATTGATGGAGCGAAGATGAAAGACTTCGTGAGCGACGAGATGCAGTTCAAGAAGAGCGTGGACGAGCGTTTTGTTGCTCTGGATCTGAACAACGATGGTGTTCTGTCCCGGTTGGAGTTGCGAAAAGCATTCGAGTCGATGAGATTGATCGAGACCCACTTCGGCATAGAAGTGGCGACCCCGCCGGATCAGATGACGCAGCTCTACGATTCCATATTCGATAAGTTCGACTGCGACCAAAGCGAGACAGTGGATCTGGACGAGTTCAGGGAGGAGATGAAGAAGATTATGCTGGCAAAAAAAAAAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7

Protein Analysis

108

Amino Acids

12.59

Weight (kDa)

4.8

Isoelectric Point (pI)

35.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 27 - 99 1.1e-09 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 197
AclWI GGATC 3 cut(s) 105, 210, 276
AgsI TTSAA 1 cut(s) 64
AluBI AGCT 1 cut(s) 217
AluI AGCT 1 cut(s) 217
Alw26I GTCTC 2 cut(s) 161, 254
AlwI GGATC 3 cut(s) 105, 210, 276
ApeKI GCWGC 1 cut(s) 214
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
Asp700I GAANNNNTTC 1 cut(s) 38
AsuC2I CCSGG 1 cut(s) 124
AsuHPI GGTGA 1 cut(s) 22
BbvI GCAGC 1 cut(s) 226
BccI CCATC 2 cut(s) 14, 104
BcgI CGANNNNNNTGC 2 cut(s) 132, 166
BcnI CCSGG 1 cut(s) 124
BcoDI GTCTC 2 cut(s) 161, 254
BisI GCNGC 1 cut(s) 215
BlsI GCNGC 1 cut(s) 216
Bme1390I CCNGG 1 cut(s) 124
BmrFI CCNGG 1 cut(s) 124
BmsI GCATC 1 cut(s) 45
BpuMI CCSGG 1 cut(s) 124
BsaI GGTCTC 1 cut(s) 161
BseRI GAGGAG 1 cut(s) 302
BseXI GCAGC 1 cut(s) 226
BsiSI CCGG 2 cut(s) 124, 200
BslFI GGGAC 1 cut(s) 106
BsmAI GTCTC 2 cut(s) 161, 254
BsmFI GGGAC 1 cut(s) 106
BsmI GAATGC 1 cut(s) 143
Bso31I GGTCTC 1 cut(s) 161
Bsp143I GATC 4 cut(s) 97, 162, 202, 268
BspACI CCGC 1 cut(s) 197
BspPI GGATC 3 cut(s) 105, 210, 276
BspQI GCTCTTC 1 cut(s) 62
BspTNI GGTCTC 1 cut(s) 161
BssMI GATC 4 cut(s) 97, 162, 202, 268
Bst4CI ACNGT 1 cut(s) 265
Bst6I CTCTTC 1 cut(s) 62
BstC8I GCNNGC 1 cut(s) 309
BstKTI GATC 4 cut(s) 100, 165, 205, 271
BstMAI GTCTC 2 cut(s) 161, 254
BstMBI GATC 4 cut(s) 97, 162, 202, 268
BstMWI GCNNNNNNNGC 1 cut(s) 255
BstSCI CCNGG 1 cut(s) 122
BstV1I GCAGC 1 cut(s) 226
BstX2I RGATCY 2 cut(s) 97, 268
BstYI RGATCY 2 cut(s) 97, 268
BtsIMutI CAGTG 1 cut(s) 270
Cac8I GCNNGC 1 cut(s) 309
CseI GACGC 1 cut(s) 220
CviJI RGCY 1 cut(s) 217
CviKI_1 RGCY 1 cut(s) 217
DpnI GATC 4 cut(s) 99, 164, 204, 270
DpnII GATC 4 cut(s) 97, 162, 202, 268
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
Eco31I GGTCTC 1 cut(s) 161
FaiI YATR 3 cut(s) 182, 230, 305
FaqI GGGAC 1 cut(s) 106
FauI CCCGC 1 cut(s) 204
Fnu4HI GCNGC 1 cut(s) 215
Fsp4HI GCNGC 1 cut(s) 215
GluI GCNGC 1 cut(s) 215
HapII CCGG 2 cut(s) 124, 200
HgaI GACGC 1 cut(s) 220
HinfI GANTC 2 cut(s) 149, 224
HpaII CCGG 2 cut(s) 124, 200
HphI GGTGA 1 cut(s) 22
Hpy166II GTNNAC 1 cut(s) 76
Hpy188I TCNGA 2 cut(s) 102, 207
Hpy188III TCNNGA 5 cut(s) 43, 64, 95, 166, 272
Hpy8I GTNNAC 1 cut(s) 76
Hpy99I CGWCG 1 cut(s) 53
HpyCH4III ACNGT 1 cut(s) 265
HpyCH4V TGCA 1 cut(s) 58
HpyF10VI GCNNNNNNNGC 1 cut(s) 255
Kzo9I GATC 4 cut(s) 97, 162, 202, 268
LguI GCTCTTC 1 cut(s) 62
LmnI GCTCC 1 cut(s) 23
LpnPI CCDG 6 cut(s) 80, 137, 213, 257, 268, 293
Lsp1109I GCAGC 1 cut(s) 226
LweI GCATC 1 cut(s) 45
MalI GATC 4 cut(s) 99, 164, 204, 270
MboI GATC 4 cut(s) 97, 162, 202, 268
MboII GAAGA 4 cut(s) 40, 79, 307, 310
MflI RGATCY 2 cut(s) 97, 268
MluCI AATT 1 cut(s) 15
MlyI GAGTC 1 cut(s) 158
MmeI TCCRAC 1 cut(s) 108
MnlI CCTC 1 cut(s) 280
MroXI GAANNNNTTC 1 cut(s) 38
MslI CAYNNNNRTG 1 cut(s) 185
MspI CCGG 2 cut(s) 124, 200
MspR9I CCNGG 1 cut(s) 124
Mva1269I GAATGC 1 cut(s) 143
MwoI GCNNNNNNNGC 1 cut(s) 255
NciI CCSGG 1 cut(s) 124
NdeII GATC 4 cut(s) 97, 162, 202, 268
PciSI GCTCTTC 1 cut(s) 62
PcsI WCGNNNNNNNCGW 2 cut(s) 48, 240
PctI GAATGC 1 cut(s) 143
PdmI GAANNNNTTC 1 cut(s) 38
PfeI GAWTC 1 cut(s) 224
PkrI GCNGC 1 cut(s) 216
PleI GAGTC 1 cut(s) 157
PpsI GAGTC 1 cut(s) 157
PsuI RGATCY 2 cut(s) 97, 268
RseI CAYNNNNRTG 1 cut(s) 185
SapI GCTCTTC 1 cut(s) 62
SatI GCNGC 1 cut(s) 215
Sau3AI GATC 4 cut(s) 97, 162, 202, 268
SchI GAGTC 1 cut(s) 158
ScrFI CCNGG 1 cut(s) 124
SetI ASST 1 cut(s) 219
SfaNI GCATC 1 cut(s) 45
SmiMI CAYNNNNRTG 1 cut(s) 185
Sse9I AATT 1 cut(s) 15
SsiI CCGC 1 cut(s) 197
StyD4I CCNGG 1 cut(s) 122
TaaI ACNGT 1 cut(s) 265
TaqI TCGA 5 cut(s) 147, 152, 165, 234, 243
TasI AATT 1 cut(s) 15
TfiI GAWTC 1 cut(s) 224
TscAI CASTG 1 cut(s) 270
TseI GCWGC 1 cut(s) 214
TspDTI ATGAA 2 cut(s) 47, 308
TspRI CASTG 1 cut(s) 270
XmnI GAANNNNTTC 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.