Rmu_sc0000104.1_g000006

EF-hand domain pair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000104.1
OG0004510
Physical Location & Seq
Reverse (-)
20725 .. 21060
336 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000104.1_g000006.1.cds

Sequence Viewer

Length: 336 bp
atgggagtggtgataattgatggaacgaagatgaaagacttcgtgagcgacgaggtgcagttcaagaagagcgtggacgagcgttttagtgctctggatctgaacaacgatggtgttctgtcccggtcggagttgcggaaagcattcgagtcgatgagattgatcgagacccacttcagcatagaagtggcgaccccgccggatcagatgacgcagctctatgattccatattcgataagttcgactgcgaccagagcgggacagtggatctggacgagttcaaggaggagatgaagaagattatgctggcaaaaaaaaagatagaaagctcctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7

Protein Analysis

111

Amino Acids

12.74

Weight (kDa)

4.7

Isoelectric Point (pI)

41.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 258
AciI CCGC 3 cut(s) 136, 197, 258
AclWI GGATC 3 cut(s) 105, 210, 276
AcuI CTGAAG 1 cut(s) 160
AgsI TTSAA 2 cut(s) 64, 283
AluBI AGCT 2 cut(s) 217, 330
AluI AGCT 2 cut(s) 217, 330
Alw21I GWGCWC 1 cut(s) 94
Alw26I GTCTC 1 cut(s) 161
AlwI GGATC 3 cut(s) 105, 210, 276
ApeKI GCWGC 1 cut(s) 214
Asp700I GAANNNNTTC 2 cut(s) 38, 143
AsuC2I CCSGG 1 cut(s) 124
AsuHPI GGTGA 1 cut(s) 22
Bbv12I GWGCWC 1 cut(s) 94
BbvI GCAGC 1 cut(s) 226
BccI CCATC 2 cut(s) 14, 104
BcgI CGANNNNNNTGC 2 cut(s) 132, 166
BcnI CCSGG 1 cut(s) 124
BcoDI GTCTC 1 cut(s) 161
BfaI CTAG 1 cut(s) 334
BisI GCNGC 1 cut(s) 215
BlsI GCNGC 1 cut(s) 216
Bme1390I CCNGG 1 cut(s) 124
BmrFI CCNGG 1 cut(s) 124
BpuMI CCSGG 1 cut(s) 124
BsaI GGTCTC 1 cut(s) 161
BseRI GAGGAG 1 cut(s) 302
BseXI GCAGC 1 cut(s) 226
BsgI GTGCAG 1 cut(s) 77
Bsh1285I CGRYCG 1 cut(s) 128
BsiEI CGRYCG 1 cut(s) 128
BsiHKAI GWGCWC 1 cut(s) 94
BsiSI CCGG 2 cut(s) 124, 200
BslFI GGGAC 2 cut(s) 106, 274
BsmAI GTCTC 1 cut(s) 161
BsmFI GGGAC 2 cut(s) 106, 274
BsmI GAATGC 1 cut(s) 143
Bso31I GGTCTC 1 cut(s) 161
Bsp1286I GDGCHC 1 cut(s) 94
Bsp143I GATC 4 cut(s) 97, 162, 202, 268
BspACI CCGC 3 cut(s) 136, 197, 258
BspPI GGATC 3 cut(s) 105, 210, 276
BspQI GCTCTTC 1 cut(s) 62
BspTNI GGTCTC 1 cut(s) 161
BsrBI CCGCTC 1 cut(s) 258
BssMI GATC 4 cut(s) 97, 162, 202, 268
Bst4CI ACNGT 1 cut(s) 265
Bst6I CTCTTC 1 cut(s) 62
BstC8I GCNNGC 1 cut(s) 309
BstKTI GATC 4 cut(s) 100, 165, 205, 271
BstMAI GTCTC 1 cut(s) 161
BstMBI GATC 4 cut(s) 97, 162, 202, 268
BstMCI CGRYCG 1 cut(s) 128
BstMWI GCNNNNNNNGC 1 cut(s) 255
BstSCI CCNGG 1 cut(s) 122
BstV1I GCAGC 1 cut(s) 226
BstX2I RGATCY 2 cut(s) 97, 268
BstYI RGATCY 2 cut(s) 97, 268
BtsIMutI CAGTG 1 cut(s) 270
Cac8I GCNNGC 1 cut(s) 309
CseI GACGC 1 cut(s) 220
CviJI RGCY 2 cut(s) 217, 330
CviKI_1 RGCY 2 cut(s) 217, 330
DpnI GATC 4 cut(s) 99, 164, 204, 270
DpnII GATC 4 cut(s) 97, 162, 202, 268
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
Eco31I GGTCTC 1 cut(s) 161
Eco57I CTGAAG 1 cut(s) 160
FaiI YATR 4 cut(s) 182, 222, 230, 305
FaqI GGGAC 2 cut(s) 106, 274
FauI CCCGC 2 cut(s) 204, 251
Fnu4HI GCNGC 1 cut(s) 215
Fsp4HI GCNGC 1 cut(s) 215
FspBI CTAG 1 cut(s) 334
GluI GCNGC 1 cut(s) 215
HapII CCGG 2 cut(s) 124, 200
HgaI GACGC 1 cut(s) 220
HinfI GANTC 2 cut(s) 149, 224
HpaII CCGG 2 cut(s) 124, 200
HphI GGTGA 1 cut(s) 22
Hpy166II GTNNAC 1 cut(s) 76
Hpy188I TCNGA 3 cut(s) 102, 130, 207
Hpy188III TCNNGA 5 cut(s) 43, 64, 95, 166, 272
Hpy8I GTNNAC 1 cut(s) 76
Hpy99I CGWCG 1 cut(s) 53
HpyCH4III ACNGT 1 cut(s) 265
HpyCH4V TGCA 1 cut(s) 58
HpyF10VI GCNNNNNNNGC 1 cut(s) 255
Kzo9I GATC 4 cut(s) 97, 162, 202, 268
LguI GCTCTTC 1 cut(s) 62
LmnI GCTCC 1 cut(s) 335
LpnPI CCDG 6 cut(s) 80, 137, 213, 257, 266, 293
Lsp1109I GCAGC 1 cut(s) 226
MaeI CTAG 1 cut(s) 334
MalI GATC 4 cut(s) 99, 164, 204, 270
MbiI CCGCTC 1 cut(s) 258
MboI GATC 4 cut(s) 97, 162, 202, 268
MboII GAAGA 4 cut(s) 40, 79, 307, 310
MflI RGATCY 2 cut(s) 97, 268
MhlI GDGCHC 1 cut(s) 94
MluCI AATT 1 cut(s) 15
MlyI GAGTC 1 cut(s) 158
MmeI TCCRAC 1 cut(s) 108
MnlI CCTC 2 cut(s) 46, 280
MroXI GAANNNNTTC 2 cut(s) 38, 143
MslI CAYNNNNRTG 1 cut(s) 185
MspI CCGG 2 cut(s) 124, 200
MspR9I CCNGG 1 cut(s) 124
Mva1269I GAATGC 1 cut(s) 143
MwoI GCNNNNNNNGC 1 cut(s) 255
NciI CCSGG 1 cut(s) 124
NdeII GATC 4 cut(s) 97, 162, 202, 268
PciSI GCTCTTC 1 cut(s) 62
PcsI WCGNNNNNNNCGW 2 cut(s) 48, 240
PctI GAATGC 1 cut(s) 143
PdmI GAANNNNTTC 2 cut(s) 38, 143
PfeI GAWTC 1 cut(s) 224
PkrI GCNGC 1 cut(s) 216
PleI GAGTC 1 cut(s) 157
PpsI GAGTC 1 cut(s) 157
PsuI RGATCY 2 cut(s) 97, 268
RseI CAYNNNNRTG 1 cut(s) 185
SapI GCTCTTC 1 cut(s) 62
SatI GCNGC 1 cut(s) 215
Sau3AI GATC 4 cut(s) 97, 162, 202, 268
SchI GAGTC 1 cut(s) 158
ScrFI CCNGG 1 cut(s) 124
SduI GDGCHC 1 cut(s) 94
SetI ASST 3 cut(s) 57, 219, 332
SmiMI CAYNNNNRTG 1 cut(s) 185
Sse9I AATT 1 cut(s) 15
SsiI CCGC 3 cut(s) 136, 197, 258
SspMI CTAG 1 cut(s) 334
StyD4I CCNGG 1 cut(s) 122
TaaI ACNGT 1 cut(s) 265
TaqI TCGA 5 cut(s) 147, 152, 165, 234, 243
TasI AATT 1 cut(s) 15
TfiI GAWTC 1 cut(s) 224
TscAI CASTG 1 cut(s) 270
TseI GCWGC 1 cut(s) 214
TspDTI ATGAA 2 cut(s) 47, 308
TspRI CASTG 1 cut(s) 270
XmnI GAANNNNTTC 2 cut(s) 38, 143
XspI CTAG 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.