Rh6BG517000

EF-hand domain pair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0004510
Physical Location & Seq
Reverse (-)
71084428 .. 71085396
969 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG517000.1

Sequence Viewer

Length: 276 bp
ATGGGTGTGGTGATAATTGACGGAACGACGGTGAAGGACTTCGTGAGCGACGAGGTGCAGTTCAAGAAGAGCGTGGACGAGCGTTTTGCTGCTCTAGATCTGAACAACGATGGTGTTCTGTCCCGGTCGGAGTTGCGGAAAGCATTCGAGTCGATGAGATTGATCGAGATCCACTTCGGCATAGAAGTGGCGACCCCGCCGGATCAGCTGACGCAGCTCTACGATTCCATATTCAATAAGTTCGACTGCGACCAGAGCGGGACGATACAACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7

Protein Analysis

91

Amino Acids

10.27

Weight (kDa)

4.44

Isoelectric Point (pI)

44.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 27 - 89 1.4e-08 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 258
AciI CCGC 3 cut(s) 136, 197, 258
AclWI GGATC 2 cut(s) 163, 210
AgsI TTSAA 2 cut(s) 64, 235
AluBI AGCT 2 cut(s) 208, 217
AluI AGCT 2 cut(s) 208, 217
AlwI GGATC 2 cut(s) 163, 210
ApeKI GCWGC 2 cut(s) 89, 214
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
Asp700I GAANNNNTTC 2 cut(s) 38, 143
AsuC2I CCSGG 1 cut(s) 124
AsuHPI GGTGA 2 cut(s) 22, 43
BaeI ACNNNNGTAYC 1 cut(s) 257
BbvI GCAGC 2 cut(s) 76, 226
BccI CCATC 1 cut(s) 104
BcgI CGANNNNNNTGC 4 cut(s) 68, 102, 132, 166
BcnI CCSGG 1 cut(s) 124
BfaI CTAG 1 cut(s) 95
BglII AGATCT 1 cut(s) 97
BisI GCNGC 2 cut(s) 90, 215
BlsI GCNGC 2 cut(s) 91, 216
Bme1390I CCNGG 1 cut(s) 124
BmrFI CCNGG 1 cut(s) 124
BpuMI CCSGG 1 cut(s) 124
BsaBI GATNNNNATC 1 cut(s) 167
Bse8I GATNNNNATC 1 cut(s) 167
BseJI GATNNNNATC 1 cut(s) 167
BseXI GCAGC 2 cut(s) 76, 226
BsgI GTGCAG 1 cut(s) 77
Bsh1285I CGRYCG 1 cut(s) 128
BsiEI CGRYCG 1 cut(s) 128
BsiSI CCGG 2 cut(s) 124, 200
BslFI GGGAC 1 cut(s) 106
BsmFI GGGAC 1 cut(s) 106
BsmI GAATGC 1 cut(s) 143
Bsp143I GATC 4 cut(s) 97, 162, 168, 202
BspACI CCGC 3 cut(s) 136, 197, 258
BspPI GGATC 2 cut(s) 163, 210
BspQI GCTCTTC 1 cut(s) 62
BsrBI CCGCTC 1 cut(s) 258
BssMI GATC 4 cut(s) 97, 162, 168, 202
Bst4CI ACNGT 2 cut(s) 31, 273
Bst6I CTCTTC 1 cut(s) 62
BstKTI GATC 4 cut(s) 100, 165, 171, 205
BstMBI GATC 4 cut(s) 97, 162, 168, 202
BstMCI CGRYCG 1 cut(s) 128
BstMWI GCNNNNNNNGC 3 cut(s) 205, 214, 255
BstSCI CCNGG 1 cut(s) 122
BstV1I GCAGC 2 cut(s) 76, 226
BstX2I RGATCY 2 cut(s) 97, 168
BstYI RGATCY 2 cut(s) 97, 168
CseI GACGC 1 cut(s) 220
CviJI RGCY 2 cut(s) 208, 217
CviKI_1 RGCY 2 cut(s) 208, 217
DpnI GATC 4 cut(s) 99, 164, 170, 204
DpnII GATC 4 cut(s) 97, 162, 168, 202
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
FaiI YATR 2 cut(s) 182, 230
FaqI GGGAC 1 cut(s) 106
FauI CCCGC 2 cut(s) 204, 251
Fnu4HI GCNGC 2 cut(s) 90, 215
Fsp4HI GCNGC 2 cut(s) 90, 215
FspBI CTAG 1 cut(s) 95
GluI GCNGC 2 cut(s) 90, 215
HapII CCGG 2 cut(s) 124, 200
HgaI GACGC 1 cut(s) 220
HinfI GANTC 2 cut(s) 149, 224
HpaII CCGG 2 cut(s) 124, 200
HphI GGTGA 2 cut(s) 22, 43
Hpy166II GTNNAC 1 cut(s) 76
Hpy188I TCNGA 2 cut(s) 102, 130
Hpy188III TCNNGA 4 cut(s) 43, 64, 95, 166
Hpy8I GTNNAC 1 cut(s) 76
Hpy99I CGWCG 2 cut(s) 31, 53
HpyAV CCTTC 1 cut(s) 28
HpyCH4III ACNGT 2 cut(s) 31, 273
HpyCH4V TGCA 1 cut(s) 58
HpyF10VI GCNNNNNNNGC 3 cut(s) 205, 214, 255
Kzo9I GATC 4 cut(s) 97, 162, 168, 202
LguI GCTCTTC 1 cut(s) 62
LpnPI CCDG 3 cut(s) 137, 213, 266
Lsp1109I GCAGC 2 cut(s) 76, 226
MaeI CTAG 1 cut(s) 95
MalI GATC 4 cut(s) 99, 164, 170, 204
MbiI CCGCTC 1 cut(s) 258
MboI GATC 4 cut(s) 97, 162, 168, 202
MboII GAAGA 1 cut(s) 79
MflI RGATCY 2 cut(s) 97, 168
MluCI AATT 1 cut(s) 15
MlyI GAGTC 1 cut(s) 158
MmeI TCCRAC 1 cut(s) 108
MnlI CCTC 1 cut(s) 46
MroXI GAANNNNTTC 2 cut(s) 38, 143
MslI CAYNNNNRTG 1 cut(s) 185
MspA1I CMGCKG 1 cut(s) 208
MspI CCGG 2 cut(s) 124, 200
MspR9I CCNGG 1 cut(s) 124
Mva1269I GAATGC 1 cut(s) 143
MwoI GCNNNNNNNGC 3 cut(s) 205, 214, 255
NciI CCSGG 1 cut(s) 124
NdeII GATC 4 cut(s) 97, 162, 168, 202
PciSI GCTCTTC 1 cut(s) 62
PcsI WCGNNNNNNNCGW 1 cut(s) 48
PctI GAATGC 1 cut(s) 143
PdmI GAANNNNTTC 2 cut(s) 38, 143
PfeI GAWTC 1 cut(s) 224
PkrI GCNGC 2 cut(s) 91, 216
PleI GAGTC 1 cut(s) 157
PpsI GAGTC 1 cut(s) 157
PsuI RGATCY 2 cut(s) 97, 168
PvuII CAGCTG 1 cut(s) 208
RseI CAYNNNNRTG 1 cut(s) 185
SapI GCTCTTC 1 cut(s) 62
SatI GCNGC 2 cut(s) 90, 215
Sau3AI GATC 4 cut(s) 97, 162, 168, 202
SchI GAGTC 1 cut(s) 158
ScrFI CCNGG 1 cut(s) 124
SetI ASST 3 cut(s) 57, 210, 219
SmiMI CAYNNNNRTG 1 cut(s) 185
Sse9I AATT 1 cut(s) 15
SsiI CCGC 3 cut(s) 136, 197, 258
SspMI CTAG 1 cut(s) 95
StyD4I CCNGG 1 cut(s) 122
TaaI ACNGT 2 cut(s) 31, 273
TaqI TCGA 4 cut(s) 147, 152, 165, 243
TasI AATT 1 cut(s) 15
TfiI GAWTC 1 cut(s) 224
TseI GCWGC 2 cut(s) 89, 214
TspGWI ACGGA 1 cut(s) 36
XbaI TCTAGA 1 cut(s) 94
XmnI GAANNNNTTC 2 cut(s) 38, 143
XspI CTAG 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.