Prupe.2G002300_v2.0.a1

EF-hand domain pair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0004510
Physical Location & Seq
Forward (+)
308800 .. 309426
627 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G002300.1

Sequence Viewer

Length: 420 bp
ATGGGCGTGGTGATCATCGACGGAACTACAGTGAGGGAGTTCGTGAAAGAGGAGGCGCAGTTCAAGAAGAGCGTTGACGAGCAGTTTGTGGCTCTGGACCTTAACAGCGACGGCGTTCTGTCGCGGGCGGAGCTACGCAAAGCGTTCGAGTCGCTGAGGCTGATTGAGGCCCACTTCGGCATCGACGTGGCCACCCCACCGGAGCAGCTGACTCAGCTCTACGACTCCATATTCGACAAGTTCGACGCCGACCAGAGCGGGACGGTGGATCTCGACGAGTTCAGGGAGGAGATGAAGAAGATTATGCTGGCCATCGCCGACGGGCTCGGCTCATCCCCTATTCAGATGGTTCTGGAGGACGACGACCAAAACTTTCTCAAGAAGGCTGCCGATCTCGAGGCTTCCAAGACCTCCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7

Protein Analysis

140

Amino Acids

15.49

Weight (kDa)

4.33

Isoelectric Point (pI)

29.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 258
AccII CGCG 1 cut(s) 124
AciI CCGC 3 cut(s) 124, 128, 258
AclWI GGATC 1 cut(s) 276
AcoI YGGCCR 2 cut(s) 189, 309
AcyI GRCGYC 1 cut(s) 246
AgsI TTSAA 1 cut(s) 64
AjiI CACGTC 1 cut(s) 187
AloI GAACNNNNNNTCC 2 cut(s) 44, 76
AluBI AGCT 3 cut(s) 133, 208, 217
AluI AGCT 3 cut(s) 133, 208, 217
AlwI GGATC 1 cut(s) 276
Ama87I CYCGRG 1 cut(s) 395
AoxI GGCC 3 cut(s) 168, 189, 309
ApeKI GCWGC 2 cut(s) 205, 386
ArsI GACNNNNNNTTYG 4 cut(s) 68, 100, 215, 247
AspLEI GCGC 1 cut(s) 58
AspS9I GGNCC 2 cut(s) 97, 169
AsuHPI GGTGA 1 cut(s) 22
AvaI CYCGRG 1 cut(s) 395
AvaII GGWCC 1 cut(s) 97
BalI TGGCCA 2 cut(s) 191, 311
BanII GRGCYC 1 cut(s) 327
BbvCI CCTCAGC 1 cut(s) 155
BbvI GCAGC 2 cut(s) 217, 373
BccI CCATC 2 cut(s) 320, 340
BceAI ACGGC 1 cut(s) 127
BcgI CGANNNNNNTGC 2 cut(s) 127, 161
BclI TGATCA 1 cut(s) 12
BfmI CTRYAG 1 cut(s) 27
BisI GCNGC 2 cut(s) 206, 387
BlsI GCNGC 2 cut(s) 207, 388
Bme18I GGWCC 1 cut(s) 97
BmeT110I CYCGRG 1 cut(s) 395
BmgBI CACGTC 1 cut(s) 187
BmgT120I GGNCC 2 cut(s) 97, 169
BmsI GCATC 1 cut(s) 189
BpmI CTGGAG 1 cut(s) 374
Bpu10I CCTNAGC 1 cut(s) 155
BpuEI CTTGAG 1 cut(s) 362
BsaHI GRCGYC 1 cut(s) 246
BsaWI WCCGGW 1 cut(s) 199
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
BseGI GGATG 1 cut(s) 332
BseMII CTCAG 2 cut(s) 146, 227
BseRI GAGGAG 2 cut(s) 65, 302
BseXI GCAGC 2 cut(s) 217, 373
Bsh1236I CGCG 1 cut(s) 124
BshFI GGCC 3 cut(s) 170, 191, 311
BsiHKCI CYCGRG 1 cut(s) 395
BsiSI CCGG 1 cut(s) 200
BslFI GGGAC 1 cut(s) 274
BsmFI GGGAC 1 cut(s) 274
BsnI GGCC 3 cut(s) 170, 191, 311
BsoBI CYCGRG 1 cut(s) 395
Bsp1286I GDGCHC 1 cut(s) 327
Bsp143I GATC 3 cut(s) 12, 268, 391
BspACI CCGC 3 cut(s) 124, 128, 258
BspANI GGCC 3 cut(s) 170, 191, 311
BspCNI CTCAG 2 cut(s) 147, 226
BspFNI CGCG 1 cut(s) 124
BspPI GGATC 1 cut(s) 276
BspQI GCTCTTC 1 cut(s) 62
BsrBI CCGCTC 1 cut(s) 258
BssMI GATC 3 cut(s) 12, 268, 391
BssNI GRCGYC 1 cut(s) 246
Bst4CI ACNGT 2 cut(s) 31, 265
Bst6I CTCTTC 1 cut(s) 62
BstACI GRCGYC 1 cut(s) 246
BstC8I GCNNGC 2 cut(s) 126, 309
BstDEI CTNAG 2 cut(s) 155, 213
BstF5I GGATG 1 cut(s) 332
BstFNI CGCG 1 cut(s) 124
BstHHI GCGC 1 cut(s) 58
BstKTI GATC 3 cut(s) 15, 271, 394
BstMBI GATC 3 cut(s) 12, 268, 391
BstMWI GCNNNNNNNGC 2 cut(s) 130, 214
BstSFI CTRYAG 1 cut(s) 27
BstUI CGCG 1 cut(s) 124
BstV1I GCAGC 2 cut(s) 217, 373
BstX2I RGATCY 1 cut(s) 268
BstYI RGATCY 1 cut(s) 268
BsuRI GGCC 3 cut(s) 170, 191, 311
BtgZI GCGATG 1 cut(s) 298
BtrI CACGTC 1 cut(s) 187
BtsCI GGATG 1 cut(s) 332
BtsIMutI CAGTG 1 cut(s) 36
Cac8I GCNNGC 2 cut(s) 126, 309
CfoI GCGC 1 cut(s) 58
Cfr13I GGNCC 2 cut(s) 97, 169
CseI GACGC 1 cut(s) 254
DdeI CTNAG 2 cut(s) 155, 213
DpnI GATC 3 cut(s) 14, 270, 393
DpnII GATC 3 cut(s) 12, 268, 391
EaeI YGGCCR 2 cut(s) 189, 309
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
EciI GGCGGA 1 cut(s) 143
Eco24I GRGCYC 1 cut(s) 327
Eco47I GGWCC 1 cut(s) 97
Eco88I CYCGRG 1 cut(s) 395
EcoT38I GRGCYC 1 cut(s) 327
FaiI YATR 2 cut(s) 230, 305
FaqI GGGAC 1 cut(s) 274
FauI CCCGC 2 cut(s) 117, 251
FbaI TGATCA 1 cut(s) 12
Fnu4HI GCNGC 2 cut(s) 206, 387
FokI GGATG 1 cut(s) 319
FriOI GRGCYC 1 cut(s) 327
Fsp4HI GCNGC 2 cut(s) 206, 387
GlaI GCGC 1 cut(s) 57
GluI GCNGC 2 cut(s) 206, 387
GsuI CTGGAG 1 cut(s) 374
HaeIII GGCC 3 cut(s) 170, 191, 311
HapII CCGG 1 cut(s) 200
HgaI GACGC 1 cut(s) 254
HhaI GCGC 1 cut(s) 58
Hin1I GRCGYC 1 cut(s) 246
Hin6I GCGC 1 cut(s) 56
HinP1I GCGC 1 cut(s) 56
HincII GTYRAC 1 cut(s) 76
HindII GTYRAC 1 cut(s) 76
HinfI GANTC 3 cut(s) 149, 211, 224
HpaII CCGG 1 cut(s) 200
HphI GGTGA 1 cut(s) 22
Hpy166II GTNNAC 1 cut(s) 76
Hpy188I TCNGA 2 cut(s) 345, 419
Hpy188III TCNNGA 7 cut(s) 43, 64, 95, 272, 353, 379, 395
Hpy8I GTNNAC 1 cut(s) 76
Hpy99I CGWCG 7 cut(s) 23, 113, 188, 248, 278, 323, 365
HpyAV CCTTC 1 cut(s) 376
HpyCH4III ACNGT 2 cut(s) 31, 265
HpyCH4IV ACGT 1 cut(s) 186
HpyF10VI GCNNNNNNNGC 2 cut(s) 130, 214
HpyF3I CTNAG 2 cut(s) 155, 213
HpySE526I ACGT 1 cut(s) 186
Hsp92I GRCGYC 1 cut(s) 246
HspAI GCGC 1 cut(s) 56
Ksp22I TGATCA 1 cut(s) 12
Kzo9I GATC 3 cut(s) 12, 268, 391
LguI GCTCTTC 1 cut(s) 62
LmnI GCTCC 2 cut(s) 130, 202
LpnPI CCDG 6 cut(s) 80, 213, 266, 268, 293, 338
Lsp1109I GCAGC 2 cut(s) 217, 373
LweI GCATC 1 cut(s) 189
MaeII ACGT 1 cut(s) 186
MalI GATC 3 cut(s) 14, 270, 393
MbiI CCGCTC 1 cut(s) 258
MboI GATC 3 cut(s) 12, 268, 391
MboII GAAGA 3 cut(s) 79, 307, 310
MflI RGATCY 1 cut(s) 268
MhlI GDGCHC 1 cut(s) 327
MlsI TGGCCA 2 cut(s) 191, 311
MluNI TGGCCA 2 cut(s) 191, 311
MlyI GAGTC 3 cut(s) 158, 205, 218
MnlI CCTC 8 cut(s) 27, 43, 46, 150, 160, 280, 349, 391
Mox20I TGGCCA 2 cut(s) 191, 311
MscI TGGCCA 2 cut(s) 191, 311
MseI TTAA 1 cut(s) 102
MslI CAYNNNNRTG 1 cut(s) 185
Msp20I TGGCCA 2 cut(s) 191, 311
MspA1I CMGCKG 1 cut(s) 208
MspI CCGG 1 cut(s) 200
MvnI CGCG 1 cut(s) 124
MwoI GCNNNNNNNGC 2 cut(s) 130, 214
NdeII GATC 3 cut(s) 12, 268, 391
NmeAIII GCCGAG 1 cut(s) 306
PaeR7I CTCGAG 1 cut(s) 395
PciSI GCTCTTC 1 cut(s) 62
PcsI WCGNNNNNNNCGW 2 cut(s) 183, 240
PkrI GCNGC 2 cut(s) 207, 388
PleI GAGTC 3 cut(s) 157, 205, 218
PpsI GAGTC 3 cut(s) 157, 205, 218
PspPI GGNCC 2 cut(s) 97, 169
PsuI RGATCY 1 cut(s) 268
PvuII CAGCTG 1 cut(s) 208
RseI CAYNNNNRTG 1 cut(s) 185
SapI GCTCTTC 1 cut(s) 62
SaqAI TTAA 1 cut(s) 102
SatI GCNGC 2 cut(s) 206, 387
Sau3AI GATC 3 cut(s) 12, 268, 391
Sau96I GGNCC 2 cut(s) 97, 169
SchI GAGTC 3 cut(s) 158, 205, 218
SduI GDGCHC 1 cut(s) 327
SetI ASST 6 cut(s) 102, 135, 189, 210, 219, 413
SfaNI GCATC 1 cut(s) 189
SfcI CTRYAG 1 cut(s) 27
Sfr274I CTCGAG 1 cut(s) 395
SinI GGWCC 1 cut(s) 97
SlaI CTCGAG 1 cut(s) 395
SmiMI CAYNNNNRTG 1 cut(s) 185
SmlI CTYRAG 2 cut(s) 377, 395
SmoI CTYRAG 2 cut(s) 377, 395
SsiI CCGC 3 cut(s) 124, 128, 258
TaaI ACNGT 2 cut(s) 31, 265
TaiI ACGT 1 cut(s) 189
TaqI TCGA 7 cut(s) 18, 147, 183, 234, 243, 273, 396
Tru1I TTAA 1 cut(s) 102
Tru9I TTAA 1 cut(s) 102
TscAI CASTG 1 cut(s) 36
TseI GCWGC 2 cut(s) 205, 386
TspDTI ATGAA 1 cut(s) 308
TspGWI ACGGA 1 cut(s) 36
TspRI CASTG 1 cut(s) 36
VpaK11BI GGWCC 1 cut(s) 97
XhoI CTCGAG 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.