Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0004510

Physical Location & Seq

Genomic Coordinates Reverse (-)

37411165 .. 37411620

Gene Structure 456 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1319800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 456 bp

ATGGGAGTGGTGATAATCGACGGAAGCACTGTGAGGGACTTCGTGAGTGACGAGGCTCAGTTCAAGAAGAGCGTCGACGACCAGTTTGCCGCCCTCGACCTCAACAGCGACGGCGTCCTCTCCCGGTTGGAGCTCCGCAAAGCCTTCGAGTCCCTCAGGCTCATCGAGGCCCACTTCGGCATCGATGTGGCCACCCCGCCGGAGCAGCTGACGCAGCTGTACGACTCCATATTCGACAAGTTCGACAGCGACCAGAGTGGAACGGTGGATCTGGAAGAGTTCAGGGAGGAGATGAAGAAGATTATGCTGGCCATCGCCGACGGGCTGGGCTCCTCCCCCATCCAGATGGTTCTGGAGGACGACGACCAGAACTTGCTCAAGAAAGCTGCAGATCTGGAGATTGCCAAGACCTCTCACTCAATCTCCTCCGCCGGCACTGGCGCTGCTGGCTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_7

Protein Analysis

152

Amino Acids

16.43

Weight (kDa)

4.3

Isoelectric Point (pI)

30.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_7	PF13499	27 - 99	2.2e-10	EF-hand domain pair
EF-hand_1	PF00036	75 - 100	1.6e-06	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004510)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G44310
fragaria_vesca	FvH4_7g00040
malus_domestica	MD02G1319800.v1.1
prunus_persica	Prupe.2G002300_v2.0.a1
pyrus_communis	pycom02g26780
rosa_chinensis	RchiOBHm_Chr1g0313211 RchiOBHm_Chr1g0331981 RchiOBHm_Chr4g0404011 RchiOBHm_Chr5g0065041
rosa_laevigata	RLG00000013113 RLG00000020975 RLG00000030815
rosa_multiflora	Rmu_sc0000104.1_g000006 Rmu_sc0003005.1_g000020 Rmu_sc0003720.1_g000017 Rmu_sc0022605.1_g000001 Rmu_sc0027690.1_g000003
rosa_roxburghii	Rroxscaffold_4G00332380
rosa_rugosa	Rorug06G0096100 RorugPtG0001600
rosa_samantha	Rh1AG001700 Rh1CG003700 Rh1CG006300 Rh1CG115200 Rh1DG001600 Rh5DG507900 Rh6BG517000 Rh6CG522900
rosa_wichuraiana	Rw1G000080 Rw4G006740 Rw5G034440 Rw6G033590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	75
AciI	CCGC	4 cut(s)	90, 136, 197, 429
AclWI	GGATC	1 cut(s)	276
AcoI	YGGCCR	2 cut(s)	189, 309
AcyI	GRCGYC	1 cut(s)	114
AfaI	GTAC	1 cut(s)	221
AgsI	TTSAA	1 cut(s)	64
AluBI	AGCT	4 cut(s)	133, 208, 217, 386
AluI	AGCT	4 cut(s)	133, 208, 217, 386
Alw21I	GWGCWC	1 cut(s)	135
AlwI	GGATC	1 cut(s)	276
AoxI	GGCC	3 cut(s)	168, 189, 309
ApeKI	GCWGC	4 cut(s)	205, 214, 386, 443
ArsI	GACNNNNNNTTYG	4 cut(s)	68, 100, 215, 247
AspLEI	GCGC	1 cut(s)	443
AspS9I	GGNCC	1 cut(s)	169
AsuC2I	CCSGG	1 cut(s)	124
AsuHPI	GGTGA	1 cut(s)	22
AxyI	CCTNAGG	1 cut(s)	155
BalI	TGGCCA	2 cut(s)	191, 311
BanII	GRGCYC	2 cut(s)	135, 332
Bbv12I	GWGCWC	1 cut(s)	135
BbvI	GCAGC	4 cut(s)	217, 226, 373, 430
BccI	CCATC	3 cut(s)	320, 340, 347
BceAI	ACGGC	1 cut(s)	127
BcgI	CGANNNNNNTGC	4 cut(s)	68, 102, 127, 161
BcnI	CCSGG	1 cut(s)	124
BfmI	CTRYAG	1 cut(s)	387
BfoI	RGCGCY	1 cut(s)	444
BglII	AGATCT	1 cut(s)	391
BisI	GCNGC	5 cut(s)	90, 206, 215, 387, 444
BlsI	GCNGC	5 cut(s)	91, 207, 216, 388, 445
Bme1390I	CCNGG	1 cut(s)	124
BmgT120I	GGNCC	1 cut(s)	169
BmiI	GGNNCC	1 cut(s)	331
BmrFI	CCNGG	1 cut(s)	124
BmsI	GCATC	1 cut(s)	189
BpmI	CTGGAG	2 cut(s)	374, 416
BpuEI	CTTGAG	1 cut(s)	362
BpuMI	CCSGG	1 cut(s)	124
Bsa29I	ATCGAT	1 cut(s)	183
BsaHI	GRCGYC	1 cut(s)	114
BsaXI	ACNNNNNCTCC	2 cut(s)	407, 437
Bse118I	RCCGGY	1 cut(s)	431
Bse1I	ACTGG	2 cut(s)	82, 442
Bse21I	CCTNAGG	1 cut(s)	155
BseCI	ATCGAT	1 cut(s)	183
BseGI	GGATG	1 cut(s)	339
BseMII	CTCAG	2 cut(s)	71, 169
BseNI	ACTGG	2 cut(s)	82, 442
BseRI	GAGGAG	3 cut(s)	302, 322, 415
BseXI	GCAGC	4 cut(s)	217, 226, 373, 430
BseYI	CCCAGC	1 cut(s)	325
BshFI	GGCC	3 cut(s)	170, 191, 311
BshVI	ATCGAT	1 cut(s)	183
BsiHKAI	GWGCWC	1 cut(s)	135
BsiSI	CCGG	3 cut(s)	124, 200, 432
BslFI	GGGAC	2 cut(s)	50, 136
BsmFI	GGGAC	2 cut(s)	50, 136
BsnI	GGCC	3 cut(s)	170, 191, 311
Bsp1286I	GDGCHC	2 cut(s)	135, 332
Bsp143I	GATC	2 cut(s)	268, 391
BspACI	CCGC	4 cut(s)	90, 136, 197, 429
BspANI	GGCC	3 cut(s)	170, 191, 311
BspCNI	CTCAG	2 cut(s)	70, 168
BspDI	ATCGAT	1 cut(s)	183
BspLI	GGNNCC	1 cut(s)	331
BspMAI	CTGCAG	1 cut(s)	391
BspPI	GGATC	1 cut(s)	276
BspQI	GCTCTTC	1 cut(s)	62
BsrFI	RCCGGY	1 cut(s)	431
BsrI	ACTGG	2 cut(s)	82, 442
BssAI	RCCGGY	1 cut(s)	431
BssMI	GATC	2 cut(s)	268, 391
BssNI	GRCGYC	1 cut(s)	114
Bst4CI	ACNGT	2 cut(s)	31, 265
Bst6I	CTCTTC	2 cut(s)	62, 270
BstACI	GRCGYC	1 cut(s)	114
BstC8I	GCNNGC	3 cut(s)	309, 433, 448
BstDEI	CTNAG	2 cut(s)	57, 155
BstF5I	GGATG	1 cut(s)	339
BstH2I	RGCGCY	1 cut(s)	444
BstHHI	GCGC	1 cut(s)	443
BstKTI	GATC	2 cut(s)	271, 394
BstMBI	GATC	2 cut(s)	268, 391
BstMWI	GCNNNNNNNGC	4 cut(s)	205, 211, 214, 447
BstSCI	CCNGG	1 cut(s)	122
BstSFI	CTRYAG	1 cut(s)	387
BstV1I	GCAGC	4 cut(s)	217, 226, 373, 430
BstX2I	RGATCY	2 cut(s)	268, 391
BstXI	CCANNNNNNTGG	1 cut(s)	346
BstYI	RGATCY	2 cut(s)	268, 391
Bsu15I	ATCGAT	1 cut(s)	183
Bsu36I	CCTNAGG	1 cut(s)	155
BsuRI	GGCC	3 cut(s)	170, 191, 311
BsuTUI	ATCGAT	1 cut(s)	183
BtgZI	GCGATG	1 cut(s)	298
BtsCI	GGATG	1 cut(s)	339
BtsIMutI	CAGTG	2 cut(s)	27, 435
Cac8I	GCNNGC	3 cut(s)	309, 433, 448
CfoI	GCGC	1 cut(s)	443
Cfr10I	RCCGGY	1 cut(s)	431
Cfr13I	GGNCC	1 cut(s)	169
ClaI	ATCGAT	1 cut(s)	183
CseI	GACGC	3 cut(s)	61, 103, 220
Csp6I	GTAC	1 cut(s)	220
CviQI	GTAC	1 cut(s)	220
DdeI	CTNAG	2 cut(s)	57, 155
DpnI	GATC	2 cut(s)	270, 393
DpnII	GATC	2 cut(s)	268, 391
EaeI	YGGCCR	2 cut(s)	189, 309
Eam1104I	CTCTTC	2 cut(s)	62, 270
EarI	CTCTTC	2 cut(s)	62, 270
EciI	GGCGGA	1 cut(s)	418
Ecl136II	GAGCTC	1 cut(s)	133
Eco24I	GRGCYC	2 cut(s)	135, 332
Eco53kI	GAGCTC	1 cut(s)	133
Eco81I	CCTNAGG	1 cut(s)	155
EcoICRI	GAGCTC	1 cut(s)	133
EcoT38I	GRGCYC	2 cut(s)	135, 332
FaiI	YATR	2 cut(s)	230, 305
FaqI	GGGAC	2 cut(s)	50, 136
FauI	CCCGC	1 cut(s)	204
FblI	GTMKAC	1 cut(s)	75
Fnu4HI	GCNGC	5 cut(s)	90, 206, 215, 387, 444
FokI	GGATG	1 cut(s)	326
FriOI	GRGCYC	2 cut(s)	135, 332
Fsp4HI	GCNGC	5 cut(s)	90, 206, 215, 387, 444
GlaI	GCGC	1 cut(s)	442
GluI	GCNGC	5 cut(s)	90, 206, 215, 387, 444
GsaI	CCCAGC	1 cut(s)	329
GsuI	CTGGAG	2 cut(s)	374, 416
HaeII	RGCGCY	1 cut(s)	444
HaeIII	GGCC	3 cut(s)	170, 191, 311
HapII	CCGG	3 cut(s)	124, 200, 432
HgaI	GACGC	3 cut(s)	61, 103, 220
HhaI	GCGC	1 cut(s)	443
Hin1I	GRCGYC	1 cut(s)	114
Hin6I	GCGC	1 cut(s)	441
HinP1I	GCGC	1 cut(s)	441
HincII	GTYRAC	1 cut(s)	76
HindII	GTYRAC	1 cut(s)	76
HinfI	GANTC	2 cut(s)	149, 224
HpaII	CCGG	3 cut(s)	124, 200, 432
HphI	GGTGA	1 cut(s)	22
Hpy166II	GTNNAC	1 cut(s)	76
Hpy188III	TCNNGA	7 cut(s)	43, 64, 272, 343, 353, 379, 395
Hpy8I	GTNNAC	1 cut(s)	76
Hpy99I	CGWCG	6 cut(s)	23, 77, 80, 113, 323, 365
HpyAV	CCTTC	1 cut(s)	154
HpyCH4III	ACNGT	2 cut(s)	31, 265
HpyCH4V	TGCA	1 cut(s)	389
HpyF10VI	GCNNNNNNNGC	4 cut(s)	205, 211, 214, 447
HpyF3I	CTNAG	2 cut(s)	57, 155
Hsp92I	GRCGYC	1 cut(s)	114
HspAI	GCGC	1 cut(s)	441
KroI	GCCGGC	1 cut(s)	431
KroNI	GCCGGC	1 cut(s)	433
Kzo9I	GATC	2 cut(s)	268, 391
LguI	GCTCTTC	1 cut(s)	62
LmnI	GCTCC	4 cut(s)	130, 138, 202, 335
Lsp1109I	GCAGC	4 cut(s)	217, 226, 373, 430
LweI	GCATC	1 cut(s)	189
MaeIII	GTNAC	1 cut(s)	47
MalI	GATC	2 cut(s)	270, 393
MboI	GATC	2 cut(s)	268, 391
MboII	GAAGA	4 cut(s)	79, 287, 307, 310
MflI	RGATCY	2 cut(s)	268, 391
MhlI	GDGCHC	2 cut(s)	135, 332
MlsI	TGGCCA	2 cut(s)	191, 311
MluNI	TGGCCA	2 cut(s)	191, 311
MlyI	GAGTC	2 cut(s)	158, 218
MmeI	TCCRAC	1 cut(s)	108
Mox20I	TGGCCA	2 cut(s)	191, 311
MroNI	GCCGGC	1 cut(s)	431
MscI	TGGCCA	2 cut(s)	191, 311
MslI	CAYNNNNRTG	2 cut(s)	185, 344
Msp20I	TGGCCA	2 cut(s)	191, 311
MspA1I	CMGCKG	2 cut(s)	208, 217
MspI	CCGG	3 cut(s)	124, 200, 432
MspR9I	CCNGG	1 cut(s)	124
MwoI	GCNNNNNNNGC	4 cut(s)	205, 211, 214, 447
NaeI	GCCGGC	1 cut(s)	433
NciI	CCSGG	1 cut(s)	124
NdeII	GATC	2 cut(s)	268, 391
NgoMIV	GCCGGC	1 cut(s)	431
NlaIV	GGNNCC	1 cut(s)	331
NmuCI	GTSAC	1 cut(s)	47
PciSI	GCTCTTC	1 cut(s)	62
PcsI	WCGNNNNNNNCGW	2 cut(s)	48, 240
PdiI	GCCGGC	1 cut(s)	433
PflFI	GACNNNGTC	1 cut(s)	113
PkrI	GCNGC	5 cut(s)	91, 207, 216, 388, 445
PleI	GAGTC	2 cut(s)	157, 218
PpsI	GAGTC	2 cut(s)	157, 218
Psp124BI	GAGCTC	1 cut(s)	135
PspFI	CCCAGC	1 cut(s)	325
PspN4I	GGNNCC	1 cut(s)	331
PspPI	GGNCC	1 cut(s)	169
PstI	CTGCAG	1 cut(s)	391
PsuI	RGATCY	2 cut(s)	268, 391
PsyI	GACNNNGTC	1 cut(s)	113
PvuII	CAGCTG	2 cut(s)	208, 217
RsaI	GTAC	1 cut(s)	221
RsaNI	GTAC	1 cut(s)	220
RseI	CAYNNNNRTG	2 cut(s)	185, 344
SacI	GAGCTC	1 cut(s)	135
SalI	GTCGAC	1 cut(s)	74
SapI	GCTCTTC	1 cut(s)	62
SatI	GCNGC	5 cut(s)	90, 206, 215, 387, 444
Sau3AI	GATC	2 cut(s)	268, 391
Sau96I	GGNCC	1 cut(s)	169
SchI	GAGTC	2 cut(s)	158, 218
ScrFI	CCNGG	1 cut(s)	124
SduI	GDGCHC	2 cut(s)	135, 332
SetI	ASST	6 cut(s)	102, 135, 210, 219, 388, 413
SfaNI	GCATC	1 cut(s)	189
SfcI	CTRYAG	1 cut(s)	387
SgrDI	CGTCGACG	1 cut(s)	74
SmiMI	CAYNNNNRTG	2 cut(s)	185, 344
SmlI	CTYRAG	1 cut(s)	377
SmoI	CTYRAG	1 cut(s)	377
SsiI	CCGC	4 cut(s)	90, 136, 197, 429
SstI	GAGCTC	1 cut(s)	135
StyD4I	CCNGG	1 cut(s)	122
TaaI	ACNGT	2 cut(s)	31, 265
TaqI	TCGA	8 cut(s)	18, 75, 96, 147, 165, 183, 234, 243
TauI	GCSGC	1 cut(s)	92
TscAI	CASTG	2 cut(s)	34, 442
TseFI	GTSAC	1 cut(s)	47
TseI	GCWGC	4 cut(s)	205, 214, 386, 443
Tsp45I	GTSAC	1 cut(s)	47
TspDTI	ATGAA	1 cut(s)	308
TspGWI	ACGGA	1 cut(s)	36
TspRI	CASTG	2 cut(s)	34, 442
Tth111I	GACNNNGTC	1 cut(s)	113
XmiI	GTMKAC	1 cut(s)	75

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.