RLG00000020975

EF-hand domain pair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0004510
Physical Location & Seq
Reverse (-)
70650951 .. 70653906
2956 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020975

Sequence Viewer

Length: 594 bp
ATGATCAAGAAAACGATCATGAAGATCGGGTGCCATAAGAGGCTGAGGAAGACGATGAAGAGCACAACTCCGCCACTATCGAACTCATCAAGAACTGAGGCTGCTGGCAAGACGAGGCCGGTTGGGTTTGGCGATGTTGAGCTGAGGCTGCTGGCAAGACTAGGCCGGTTGGGGTTCACAGCTCGGAGAGAATTTATGTGGGCAGACATTTACGCAGGAAAAGAAGAAAAGAAAAATCGACGGAGCCACGGACGTAAGATGGGAGTGGTGATAATTGACGGAACGACGGTGAAGGACTTCGTGAGCGACGAGGTGCAGTTCAAGAAGAGCGTGGACGAGCGTTTTGCTGCTCTGGATCTGAACAACGATGGTGTTCTATCCGGGTCGGAGCTGCGGAAAGCATTCGAGTCGATGAGATTGATCGAGACCCACTTCGGCATAGAAGTGGTGACCACGCCGGATCAGCTGACGCAGCTTTACGATTCCATATTTAATAAGTTCAATTGCGACCAGAGAGGGACGGTGGATCTGGACGAGTTGAGGGAGGAGATGAAGAGATTATGCTGGCCAAAAAAAAAGATAGAAAGCCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7

Protein Analysis

198

Amino Acids

22.56

Weight (kDa)

9.62

Isoelectric Point (pI)

46.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 113 - 184 1.2e-08 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 30
AciI CCGC 2 cut(s) 71, 394
AclWI GGATC 3 cut(s) 363, 468, 534
AcoI YGGCCR 1 cut(s) 566
AcsI RAATTY 1 cut(s) 191
AgsI TTSAA 2 cut(s) 322, 502
AluBI AGCT 5 cut(s) 142, 182, 391, 466, 475
AluI AGCT 5 cut(s) 142, 182, 391, 466, 475
Alw21I GWGCWC 1 cut(s) 65
Alw26I GTCTC 1 cut(s) 419
AlwI GGATC 3 cut(s) 363, 468, 534
AoxI GGCC 3 cut(s) 116, 163, 566
ApeKI GCWGC 5 cut(s) 101, 148, 347, 391, 472
ApoI RAATTY 1 cut(s) 191
ArsI GACNNNNNNTTYG 2 cut(s) 326, 358
Asp700I GAANNNNTTC 2 cut(s) 296, 401
AsuC2I CCSGG 1 cut(s) 382
AsuHPI GGTGA 3 cut(s) 280, 301, 460
BalI TGGCCA 1 cut(s) 568
BanI GGYRCC 1 cut(s) 30
BbsI GAAGAC 1 cut(s) 56
Bbv12I GWGCWC 1 cut(s) 65
BbvCI CCTCAGC 2 cut(s) 44, 143
BbvI GCAGC 5 cut(s) 88, 135, 334, 378, 484
BccI CCATC 2 cut(s) 253, 362
BcgI CGANNNNNNTGC 4 cut(s) 326, 360, 390, 424
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 1 cut(s) 382
BcoDI GTCTC 1 cut(s) 419
BfaI CTAG 2 cut(s) 161, 592
BisI GCNGC 5 cut(s) 102, 149, 348, 392, 473
BlsI GCNGC 5 cut(s) 103, 150, 349, 393, 474
Bme1390I CCNGG 1 cut(s) 382
BmiI GGNNCC 2 cut(s) 32, 245
BmrFI CCNGG 1 cut(s) 382
BpiI GAAGAC 1 cut(s) 56
BplI GAGNNNNNCTC 2 cut(s) 52, 84
Bpu10I CCTNAGC 2 cut(s) 44, 143
BpuMI CCSGG 1 cut(s) 382
BsaI GGTCTC 1 cut(s) 419
BsaJI CCNNGG 1 cut(s) 247
Bse118I RCCGGY 2 cut(s) 118, 165
BseDI CCNNGG 1 cut(s) 247
BseMII CTCAG 3 cut(s) 35, 87, 134
BseRI GAGGAG 1 cut(s) 560
BseXI GCAGC 5 cut(s) 88, 135, 334, 378, 484
BsgI GTGCAG 1 cut(s) 335
BshFI GGCC 3 cut(s) 118, 165, 568
BshNI GGYRCC 1 cut(s) 30
BsiHKAI GWGCWC 1 cut(s) 65
BsiSI CCGG 4 cut(s) 119, 166, 381, 458
BslFI GGGAC 1 cut(s) 532
BsmAI GTCTC 1 cut(s) 419
BsmFI GGGAC 1 cut(s) 532
BsmI GAATGC 1 cut(s) 401
BsnI GGCC 3 cut(s) 118, 165, 568
Bso31I GGTCTC 1 cut(s) 419
Bsp1286I GDGCHC 1 cut(s) 65
Bsp143I GATC 7 cut(s) 3, 15, 24, 355, 420, 460, 526
BspACI CCGC 2 cut(s) 71, 394
BspANI GGCC 3 cut(s) 118, 165, 568
BspCNI CTCAG 3 cut(s) 36, 88, 135
BspHI TCATGA 1 cut(s) 18
BspLI GGNNCC 2 cut(s) 32, 245
BspPI GGATC 3 cut(s) 363, 468, 534
BspQI GCTCTTC 2 cut(s) 53, 320
BspT107I GGYRCC 1 cut(s) 30
BspTNI GGTCTC 1 cut(s) 419
BsrFI RCCGGY 2 cut(s) 118, 165
BssAI RCCGGY 2 cut(s) 118, 165
BssECI CCNNGG 1 cut(s) 247
BssMI GATC 7 cut(s) 3, 15, 24, 355, 420, 460, 526
Bst4CI ACNGT 2 cut(s) 289, 523
Bst6I CTCTTC 3 cut(s) 53, 320, 548
BstC8I GCNNGC 3 cut(s) 106, 153, 566
BstDEI CTNAG 3 cut(s) 44, 96, 143
BstDSI CCRYGG 1 cut(s) 247
BstEII GGTNACC 1 cut(s) 448
BstKTI GATC 7 cut(s) 6, 18, 27, 358, 423, 463, 529
BstMAI GTCTC 1 cut(s) 419
BstMBI GATC 7 cut(s) 3, 15, 24, 355, 420, 460, 526
BstMWI GCNNNNNNNGC 3 cut(s) 148, 463, 472
BstPI GGTNACC 1 cut(s) 448
BstSCI CCNGG 1 cut(s) 380
BstV1I GCAGC 5 cut(s) 88, 135, 334, 378, 484
BstV2I GAAGAC 1 cut(s) 56
BstX2I RGATCY 2 cut(s) 355, 526
BstYI RGATCY 2 cut(s) 355, 526
BsuRI GGCC 3 cut(s) 118, 165, 568
BtgI CCRYGG 1 cut(s) 247
BtgZI GCGATG 1 cut(s) 147
Cac8I GCNNGC 3 cut(s) 106, 153, 566
CciI TCATGA 1 cut(s) 18
Cfr10I RCCGGY 2 cut(s) 118, 165
CseI GACGC 1 cut(s) 478
CviAII CATG 1 cut(s) 19
DdeI CTNAG 3 cut(s) 44, 96, 143
DpnI GATC 7 cut(s) 5, 17, 26, 357, 422, 462, 528
DpnII GATC 7 cut(s) 3, 15, 24, 355, 420, 460, 526
EaeI YGGCCR 1 cut(s) 566
Eam1104I CTCTTC 3 cut(s) 53, 320, 548
EarI CTCTTC 3 cut(s) 53, 320, 548
EciI GGCGGA 1 cut(s) 60
Eco31I GGTCTC 1 cut(s) 419
Eco91I GGTNACC 1 cut(s) 448
EcoO65I GGTNACC 1 cut(s) 448
FaeI CATG 1 cut(s) 22
FaiI YATR 6 cut(s) 20, 36, 197, 440, 488, 562
FaqI GGGAC 1 cut(s) 532
FatI CATG 1 cut(s) 18
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 5 cut(s) 102, 149, 348, 392, 473
Fsp4HI GCNGC 5 cut(s) 102, 149, 348, 392, 473
FspBI CTAG 2 cut(s) 161, 592
GluI GCNGC 5 cut(s) 102, 149, 348, 392, 473
HaeIII GGCC 3 cut(s) 118, 165, 568
HapII CCGG 4 cut(s) 119, 166, 381, 458
HgaI GACGC 1 cut(s) 478
Hin1II CATG 1 cut(s) 22
HinfI GANTC 2 cut(s) 407, 482
HpaII CCGG 4 cut(s) 119, 166, 381, 458
HphI GGTGA 3 cut(s) 280, 301, 460
Hpy166II GTNNAC 2 cut(s) 177, 334
Hpy188I TCNGA 3 cut(s) 186, 360, 388
Hpy188III TCNNGA 8 cut(s) 7, 19, 90, 301, 322, 353, 424, 530
Hpy8I GTNNAC 2 cut(s) 177, 334
Hpy99I CGWCG 3 cut(s) 243, 289, 311
HpyAV CCTTC 1 cut(s) 286
HpyCH4III ACNGT 2 cut(s) 289, 523
HpyCH4IV ACGT 1 cut(s) 253
HpyCH4V TGCA 1 cut(s) 316
HpyF10VI GCNNNNNNNGC 3 cut(s) 148, 463, 472
HpyF3I CTNAG 3 cut(s) 44, 96, 143
HpySE526I ACGT 1 cut(s) 253
Hsp92II CATG 1 cut(s) 22
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 7 cut(s) 3, 15, 24, 355, 420, 460, 526
LguI GCTCTTC 2 cut(s) 53, 320
LmnI GCTCC 2 cut(s) 243, 388
Lsp1109I GCAGC 5 cut(s) 88, 135, 334, 378, 484
MaeI CTAG 2 cut(s) 161, 592
MaeII ACGT 1 cut(s) 253
MaeIII GTNAC 1 cut(s) 448
MalI GATC 7 cut(s) 5, 17, 26, 357, 422, 462, 528
MboI GATC 7 cut(s) 3, 15, 24, 355, 420, 460, 526
MboII GAAGA 6 cut(s) 34, 61, 70, 236, 337, 565
MfeI CAATTG 1 cut(s) 502
MflI RGATCY 2 cut(s) 355, 526
MhlI GDGCHC 1 cut(s) 65
MlsI TGGCCA 1 cut(s) 568
MluCI AATT 3 cut(s) 191, 273, 502
MluNI TGGCCA 1 cut(s) 568
MlyI GAGTC 1 cut(s) 416
MmeI TCCRAC 1 cut(s) 366
MnlI CCTC 9 cut(s) 33, 39, 91, 108, 138, 304, 509, 534, 538
Mox20I TGGCCA 1 cut(s) 568
MroXI GAANNNNTTC 2 cut(s) 296, 401
MscI TGGCCA 1 cut(s) 568
MseI TTAA 1 cut(s) 492
MslI CAYNNNNRTG 1 cut(s) 443
Msp20I TGGCCA 1 cut(s) 568
MspA1I CMGCKG 1 cut(s) 466
MspI CCGG 4 cut(s) 119, 166, 381, 458
MspR9I CCNGG 1 cut(s) 382
MunI CAATTG 1 cut(s) 502
Mva1269I GAATGC 1 cut(s) 401
MwoI GCNNNNNNNGC 3 cut(s) 148, 463, 472
NciI CCSGG 1 cut(s) 382
NdeII GATC 7 cut(s) 3, 15, 24, 355, 420, 460, 526
NlaIII CATG 1 cut(s) 22
NlaIV GGNNCC 2 cut(s) 32, 245
NmuCI GTSAC 1 cut(s) 448
PagI TCATGA 1 cut(s) 18
PciSI GCTCTTC 2 cut(s) 53, 320
PcsI WCGNNNNNNNCGW 1 cut(s) 306
PctI GAATGC 1 cut(s) 401
PdmI GAANNNNTTC 2 cut(s) 296, 401
PfeI GAWTC 1 cut(s) 482
PkrI GCNGC 5 cut(s) 103, 150, 349, 393, 474
PleI GAGTC 1 cut(s) 415
PpsI GAGTC 1 cut(s) 415
PspEI GGTNACC 1 cut(s) 448
PspN4I GGNNCC 2 cut(s) 32, 245
PsuI RGATCY 2 cut(s) 355, 526
PvuII CAGCTG 1 cut(s) 466
RseI CAYNNNNRTG 1 cut(s) 443
SapI GCTCTTC 2 cut(s) 53, 320
SaqAI TTAA 1 cut(s) 492
SatI GCNGC 5 cut(s) 102, 149, 348, 392, 473
Sau3AI GATC 7 cut(s) 3, 15, 24, 355, 420, 460, 526
SchI GAGTC 1 cut(s) 416
ScrFI CCNGG 1 cut(s) 382
SduI GDGCHC 1 cut(s) 65
SetI ASST 7 cut(s) 144, 184, 256, 315, 393, 468, 477
SmiMI CAYNNNNRTG 1 cut(s) 443
Sse9I AATT 3 cut(s) 191, 273, 502
SsiI CCGC 2 cut(s) 71, 394
SspMI CTAG 2 cut(s) 161, 592
StyD4I CCNGG 1 cut(s) 380
TaaI ACNGT 2 cut(s) 289, 523
TaiI ACGT 1 cut(s) 256
TaqI TCGA 5 cut(s) 80, 238, 405, 410, 423
TasI AATT 3 cut(s) 191, 273, 502
TfiI GAWTC 1 cut(s) 482
Tru1I TTAA 1 cut(s) 492
Tru9I TTAA 1 cut(s) 492
TseFI GTSAC 1 cut(s) 448
TseI GCWGC 5 cut(s) 101, 148, 347, 391, 472
Tsp45I GTSAC 1 cut(s) 448
TspDTI ATGAA 3 cut(s) 35, 71, 566
TspGWI ACGGA 3 cut(s) 256, 264, 294
XapI RAATTY 1 cut(s) 191
XmnI GAANNNNTTC 2 cut(s) 296, 401
XspI CTAG 2 cut(s) 161, 592
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.