RorugPtG0001600

EF-hand domain pair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000008
OG0004510
Physical Location & Seq
Forward (+)
33871 .. 34932
1062 bp
Loading structure...
UTR
Exon/CDS
Intron
RorugPtG0001600.1

Sequence Viewer

Length: 1062 bp
ATGACTATAGCCCTTGGTAAATTTACCAAAGACGAAAATGATTTATTTGATATTATGGATGACTGGTTACGGAGGGACCGTTTCGTTTTTGTAGGTTGGTCTGGCCTATTGCTCTTTCCTTGTGCCTATTTCGCTTTAGGGGGTTGGTTCACAGGTACAACCTTTGTAACTTCATGGTATACCCATGGATTAGCAAGTTCCTATTTGGAAGGCTGTAACTTCTTAACCGCCGCAGTTTCCACCCCTGCTAACAGTTTAGCACACTCTTTGTTGTTACTATGGGGTCCTGAAGCACAAGGAGATTTTACTCGTTGGTGTCAATTGGGCGGTCTGTGGACTTTTGTCGCTCTACACGGTGCTTTCGGACTAATAGGTTTCATGTTACGTCAATTTGAACTTGCTCGATCTGTTCAATTACGACCTTATAATGCAATTGCATTCTCCGGTCCAATTGCTGTTTTTGTTTCTGTATTCCTGATTTATCCGCTAGGTCAGTCTGGTTGGTTCTTTGCGCCCAGTTTTGGTGTAGCAGCTATATTTCGATTCATCCTCTTTTTCCAAGGGTTTCATAATTGGACATTGAACCCATTTCATATGATGGGAGTTGCGGGGGTGTTGGGCGCTGCTCTGTTATGCGCTATTCATGGTGCTACCGTAGAAAATACTTTATTTGAAGATGGTGATGGTGCAAATACATTCCGTGCTTTTAACCCAACTCAAGCTGAAGAAACTTATTCCATGGTCACCGCTAACCGCTTTTGGTCCCAAATCTTTGGGGTTGCTTTTTCCAATAAACGTTGGTTACATTTCTTTATGTTATTTGTACCAGTAACCGGTTTATGGATGAGTGCTCTTGGAGTAGTCGGTCTGGCCCTGAACCTACGTGCCTATGACTTCGTTTCTCAGGAAATCCGTGCAGCGGAAGATCCTGAATTTGAGACTTTCTACACCAAAAATATTCTCTTAAACGAAGGTATTCGTGCTTGGATGGCGGCTCAAGATCAGCCTCATGAAAACCTTATATTCCCTGAGGAGGTTCTACCCCGTGGAAACGCTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7

Protein Analysis

353

Amino Acids

39.55

Weight (kDa)

5.33

Isoelectric Point (pI)

39.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Photo_RC PF00124 29 - 318 1.4e-73 Photosynthetic reaction centre protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 426
AccI GTMKAC 1 cut(s) 179
AciI CCGC 9 cut(s) 228, 231, 327, 485, 608, 747, 754, 920, 992
AclI AACGTT 1 cut(s) 796
AclWI GGATC 1 cut(s) 920
AcsI RAATTY 2 cut(s) 20, 932
AcuI CTGAAG 2 cut(s) 309, 744
AfaI GTAC 2 cut(s) 157, 825
AfiI CCNNNNNNNGG 5 cut(s) 521, 833, 840, 919, 1033
AgeI ACCGGT 1 cut(s) 833
AgsI TTSAA 4 cut(s) 395, 413, 583, 674
AluBI AGCT 2 cut(s) 533, 722
AluI AGCT 2 cut(s) 533, 722
Alw21I GWGCWC 1 cut(s) 853
Alw26I GTCTC 1 cut(s) 932
AlwI GGATC 1 cut(s) 920
AoxI GGCC 2 cut(s) 103, 870
ApeKI GCWGC 3 cut(s) 530, 623, 917
ApoI RAATTY 2 cut(s) 20, 932
AsiGI ACCGGT 1 cut(s) 833
Asp700I GAANNNNTTC 1 cut(s) 975
AspLEI GCGC 3 cut(s) 514, 623, 638
AspS9I GGNCC 5 cut(s) 76, 284, 446, 762, 871
AsuHPI GGTGA 2 cut(s) 692, 736
AvaII GGWCC 4 cut(s) 76, 284, 446, 762
AxyI CCTNAGG 1 cut(s) 1029
Bbv12I GWGCWC 1 cut(s) 853
BbvI GCAGC 3 cut(s) 542, 610, 929
BccI CCATC 4 cut(s) 592, 671, 677, 982
BcoDI GTCTC 1 cut(s) 932
BfaI CTAG 1 cut(s) 488
BfmI CTRYAG 1 cut(s) 6
BfoI RGCGCY 1 cut(s) 624
BisI GCNGC 5 cut(s) 231, 531, 624, 918, 993
BlsI GCNGC 5 cut(s) 232, 532, 625, 919, 994
Bme18I GGWCC 4 cut(s) 76, 284, 446, 762
BmgT120I GGNCC 5 cut(s) 76, 284, 446, 762, 871
BmiI GGNNCC 3 cut(s) 77, 285, 764
BmrI ACTGGG 1 cut(s) 510
BmuI ACTGGG 1 cut(s) 510
BoxI GACNNNNGTC 1 cut(s) 341
BpuEI CTTGAG 2 cut(s) 702, 981
BsaAI YACGTR 1 cut(s) 884
BsaJI CCNNGG 5 cut(s) 13, 184, 559, 738, 1045
BsaWI WCCGGW 2 cut(s) 443, 833
BsaXI ACNNNNNCTCC 2 cut(s) 64, 94
Bsc4I CCNNNNNNNGG 5 cut(s) 521, 833, 840, 919, 1033
Bse118I RCCGGY 1 cut(s) 833
Bse1I ACTGG 3 cut(s) 68, 516, 827
Bse21I CCTNAGG 1 cut(s) 1029
BseDI CCNNGG 5 cut(s) 13, 184, 559, 738, 1045
BseGI GGATG 4 cut(s) 64, 546, 849, 993
BseLI CCNNNNNNNGG 5 cut(s) 521, 833, 840, 919, 1033
BseMII CTCAG 2 cut(s) 917, 1020
BseNI ACTGG 3 cut(s) 68, 516, 827
BseRI GAGGAG 1 cut(s) 1046
BseXI GCAGC 3 cut(s) 542, 610, 929
BsgI GTGCAG 1 cut(s) 936
BshFI GGCC 2 cut(s) 105, 872
BshTI ACCGGT 1 cut(s) 833
BsiHKAI GWGCWC 1 cut(s) 853
BsiSI CCGG 2 cut(s) 444, 834
BslFI GGGAC 2 cut(s) 89, 748
BslI CCNNNNNNNGG 5 cut(s) 521, 833, 840, 919, 1033
BsmAI GTCTC 1 cut(s) 932
BsmFI GGGAC 2 cut(s) 89, 748
BsmI GAATGC 1 cut(s) 437
BsnI GGCC 2 cut(s) 105, 872
Bsp1286I GDGCHC 1 cut(s) 853
Bsp143I GATC 3 cut(s) 404, 925, 1000
Bsp19I CCATGG 2 cut(s) 184, 738
BspACI CCGC 9 cut(s) 228, 231, 327, 485, 608, 747, 754, 920, 992
BspANI GGCC 2 cut(s) 105, 872
BspCNI CTCAG 2 cut(s) 916, 1021
BspHI TCATGA 1 cut(s) 1009
BspLI GGNNCC 3 cut(s) 77, 285, 764
BspPI GGATC 1 cut(s) 920
BsrFI RCCGGY 1 cut(s) 833
BsrI ACTGG 3 cut(s) 68, 516, 827
BssAI RCCGGY 1 cut(s) 833
BssECI CCNNGG 5 cut(s) 13, 184, 559, 738, 1045
BssMI GATC 3 cut(s) 404, 925, 1000
BssNAI GTATAC 1 cut(s) 180
BssT1I CCWWGG 4 cut(s) 13, 184, 559, 738
Bst1107I GTATAC 1 cut(s) 180
Bst4CI ACNGT 4 cut(s) 80, 254, 356, 655
BstBAI YACGTR 1 cut(s) 884
BstDEI CTNAG 2 cut(s) 903, 1029
BstDSI CCRYGG 3 cut(s) 184, 738, 1045
BstEII GGTNACC 1 cut(s) 742
BstF5I GGATG 4 cut(s) 64, 546, 849, 993
BstH2I RGCGCY 1 cut(s) 624
BstHHI GCGC 3 cut(s) 514, 623, 638
BstKTI GATC 3 cut(s) 407, 928, 1003
BstMAI GTCTC 1 cut(s) 932
BstMBI GATC 3 cut(s) 404, 925, 1000
BstMWI GCNNNNNNNGC 2 cut(s) 131, 989
BstPAI GACNNNNGTC 1 cut(s) 341
BstPI GGTNACC 1 cut(s) 742
BstSFI CTRYAG 1 cut(s) 6
BstV1I GCAGC 3 cut(s) 542, 610, 929
BstX2I RGATCY 1 cut(s) 925
BstXI CCANNNNNNTGG 1 cut(s) 773
BstYI RGATCY 1 cut(s) 925
BstZ17I GTATAC 1 cut(s) 180
Bsu36I CCTNAGG 1 cut(s) 1029
BsuRI GGCC 2 cut(s) 105, 872
BtgI CCRYGG 3 cut(s) 184, 738, 1045
BtsCI GGATG 4 cut(s) 64, 546, 849, 993
CciI TCATGA 1 cut(s) 1009
CfoI GCGC 3 cut(s) 514, 623, 638
Cfr10I RCCGGY 1 cut(s) 833
Cfr13I GGNCC 5 cut(s) 76, 284, 446, 762, 871
Csp6I GTAC 2 cut(s) 156, 824
CspAI ACCGGT 1 cut(s) 833
CviAII CATG 6 cut(s) 174, 185, 379, 644, 739, 1010
CviJI RGCY 8 cut(s) 11, 105, 213, 533, 722, 872, 995, 1006
CviKI_1 RGCY 8 cut(s) 11, 105, 213, 533, 722, 872, 995, 1006
CviQI GTAC 2 cut(s) 156, 824
DdeI CTNAG 2 cut(s) 903, 1029
DpnI GATC 3 cut(s) 406, 927, 1002
DpnII GATC 3 cut(s) 404, 925, 1000
Eco130I CCWWGG 4 cut(s) 13, 184, 559, 738
Eco47I GGWCC 4 cut(s) 76, 284, 446, 762
Eco57I CTGAAG 2 cut(s) 309, 744
Eco81I CCTNAGG 1 cut(s) 1029
Eco91I GGTNACC 1 cut(s) 742
EcoO109I RGGNCCY 1 cut(s) 284
EcoO65I GGTNACC 1 cut(s) 742
EcoT14I CCWWGG 4 cut(s) 13, 184, 559, 738
ErhI CCWWGG 4 cut(s) 13, 184, 559, 738
FaeI CATG 6 cut(s) 177, 188, 382, 647, 742, 1013
FaqI GGGAC 2 cut(s) 89, 748
FatI CATG 6 cut(s) 173, 184, 378, 643, 738, 1009
FauI CCCGC 1 cut(s) 601
FauNDI CATATG 1 cut(s) 594
FblI GTMKAC 1 cut(s) 179
Fnu4HI GCNGC 5 cut(s) 231, 531, 624, 918, 993
FokI GGATG 4 cut(s) 71, 533, 856, 1000
Fsp4HI GCNGC 5 cut(s) 231, 531, 624, 918, 993
FspBI CTAG 1 cut(s) 488
GlaI GCGC 3 cut(s) 513, 622, 637
GluI GCNGC 5 cut(s) 231, 531, 624, 918, 993
HaeII RGCGCY 1 cut(s) 624
HaeIII GGCC 2 cut(s) 105, 872
HapII CCGG 2 cut(s) 444, 834
HhaI GCGC 3 cut(s) 514, 623, 638
Hin1II CATG 6 cut(s) 177, 188, 382, 647, 742, 1013
Hin6I GCGC 3 cut(s) 512, 621, 636
HinP1I GCGC 3 cut(s) 512, 621, 636
HinfI GANTC 1 cut(s) 543
HpaII CCGG 2 cut(s) 444, 834
HphI GGTGA 2 cut(s) 692, 736
Hpy166II GTNNAC 3 cut(s) 150, 180, 336
Hpy188I TCNGA 1 cut(s) 365
Hpy188III TCNNGA 6 cut(s) 287, 475, 905, 929, 998, 1010
Hpy8I GTNNAC 3 cut(s) 150, 180, 336
HpyAV CCTTC 2 cut(s) 203, 965
HpyCH4III ACNGT 4 cut(s) 80, 254, 356, 655
HpyCH4IV ACGT 3 cut(s) 385, 796, 883
HpyCH4V TGCA 4 cut(s) 431, 437, 689, 917
HpyF10VI GCNNNNNNNGC 2 cut(s) 131, 989
HpyF3I CTNAG 2 cut(s) 903, 1029
HpySE526I ACGT 3 cut(s) 385, 796, 883
Hsp92II CATG 6 cut(s) 177, 188, 382, 647, 742, 1013
HspAI GCGC 3 cut(s) 512, 621, 636
Kzo9I GATC 3 cut(s) 404, 925, 1000
Lsp1109I GCAGC 3 cut(s) 542, 610, 929
MaeI CTAG 1 cut(s) 488
MaeII ACGT 3 cut(s) 385, 796, 883
MaeIII GTNAC 8 cut(s) 66, 166, 215, 273, 381, 742, 801, 829
MalI GATC 3 cut(s) 406, 927, 1002
MboI GATC 3 cut(s) 404, 925, 1000
MboII GAAGA 3 cut(s) 686, 737, 935
MfeI CAATTG 3 cut(s) 320, 432, 450
MflI RGATCY 1 cut(s) 925
MhlI GDGCHC 1 cut(s) 853
MluCI AATT 8 cut(s) 20, 320, 389, 413, 432, 450, 571, 932
MnlI CCTC 5 cut(s) 66, 560, 1017, 1024, 1027
MroXI GAANNNNTTC 1 cut(s) 975
MseI TTAA 4 cut(s) 224, 708, 965, 1060
MspA1I CMGCKG 1 cut(s) 920
MspI CCGG 2 cut(s) 444, 834
MunI CAATTG 3 cut(s) 320, 432, 450
Mva1269I GAATGC 1 cut(s) 437
MwoI GCNNNNNNNGC 2 cut(s) 131, 989
NcoI CCATGG 2 cut(s) 184, 738
NdeI CATATG 1 cut(s) 594
NdeII GATC 3 cut(s) 404, 925, 1000
NlaIII CATG 6 cut(s) 177, 188, 382, 647, 742, 1013
NlaIV GGNNCC 3 cut(s) 77, 285, 764
NmuCI GTSAC 1 cut(s) 742
PagI TCATGA 1 cut(s) 1009
PcsI WCGNNNNNNNCGW 1 cut(s) 76
PctI GAATGC 1 cut(s) 437
PdmI GAANNNNTTC 1 cut(s) 975
PfeI GAWTC 1 cut(s) 543
PinAI ACCGGT 1 cut(s) 833
PkrI GCNGC 5 cut(s) 232, 532, 625, 919, 994
Ppu21I YACGTR 1 cut(s) 884
PpuMI RGGWCCY 1 cut(s) 284
PshAI GACNNNNGTC 1 cut(s) 341
PsiI TTATAA 1 cut(s) 426
Psp1406I AACGTT 1 cut(s) 796
Psp5II RGGWCCY 1 cut(s) 284
PspEI GGTNACC 1 cut(s) 742
PspN4I GGNNCC 3 cut(s) 77, 285, 764
PspPI GGNCC 5 cut(s) 76, 284, 446, 762, 871
PspPPI RGGWCCY 1 cut(s) 284
PsuI RGATCY 1 cut(s) 925
RsaI GTAC 2 cut(s) 157, 825
RsaNI GTAC 2 cut(s) 156, 824
SaqAI TTAA 4 cut(s) 224, 708, 965, 1060
SatI GCNGC 5 cut(s) 231, 531, 624, 918, 993
Sau3AI GATC 3 cut(s) 404, 925, 1000
Sau96I GGNCC 5 cut(s) 76, 284, 446, 762, 871
SduI GDGCHC 1 cut(s) 853
SfcI CTRYAG 1 cut(s) 6
SinI GGWCC 4 cut(s) 76, 284, 446, 762
SmlI CTYRAG 2 cut(s) 717, 996
SmoI CTYRAG 2 cut(s) 717, 996
Sse9I AATT 8 cut(s) 20, 320, 389, 413, 432, 450, 571, 932
SsiI CCGC 9 cut(s) 228, 231, 327, 485, 608, 747, 754, 920, 992
SspI AATATT 1 cut(s) 958
SspMI CTAG 1 cut(s) 488
StyI CCWWGG 4 cut(s) 13, 184, 559, 738
TaaI ACNGT 4 cut(s) 80, 254, 356, 655
TaiI ACGT 3 cut(s) 388, 799, 886
TaqI TCGA 2 cut(s) 403, 541
TaqII GACCGA 1 cut(s) 854
TasI AATT 8 cut(s) 20, 320, 389, 413, 432, 450, 571, 932
TauI GCSGC 2 cut(s) 233, 995
TfiI GAWTC 1 cut(s) 543
Tru1I TTAA 4 cut(s) 224, 708, 965, 1060
Tru9I TTAA 4 cut(s) 224, 708, 965, 1060
TseFI GTSAC 1 cut(s) 742
TseI GCWGC 3 cut(s) 530, 623, 917
Tsp45I GTSAC 1 cut(s) 742
TspDTI ATGAA 7 cut(s) 162, 367, 535, 557, 581, 632, 1026
TspGWI ACGGA 3 cut(s) 85, 689, 902
VpaK11BI GGWCC 4 cut(s) 76, 284, 446, 762
XapI RAATTY 2 cut(s) 20, 932
XmiI GTMKAC 1 cut(s) 179
XmnI GAANNNNTTC 1 cut(s) 975
XspI CTAG 1 cut(s) 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.