Rw6G033590

EF-hand domain pair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0004510
Physical Location & Seq
Reverse (-)
58159897 .. 58160307
411 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G033590.1

Sequence Viewer

Length: 411 bp
ATGGGAGTGGTGATAATTGACGGAACGACGGTGAAGGACTTCGTGAGAGACGAGGTGCAGTTCAAGAAGAGCGTGGACGAGCGTTTTGCTGCTCTGGATCTGAACAACGATGGTGTTCTGTCCCGATCGGAGCTGCGGAAAGCATTCGAGTCTATAAGATTGATCGAGACCCACTTCGGCATAGAAGTGGCGACCCCGCCGGATCAGCTGACACAGCTCTACGATTCCATATTCGATAAGTTCGACTACGACCAGAGAGGGACGGTGGATCTGGACGAGTTCAGGGAGGAGATGAAGAGATTATGCTGGCAAAAAAAAAAGATAGAAAGAGCCTTGGCGCCTACCGCCCAGAGATGCCCAGCCCCGCCTAGGCGGCCGACTTTATTCCCAGCGCCCAGCTCGCTCGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7

Protein Analysis

136

Amino Acids

15.65

Weight (kDa)

5.29

Isoelectric Point (pI)

52.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 27 - 99 2e-11 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 337
AciI CCGC 5 cut(s) 136, 197, 345, 365, 373
AclWI GGATC 3 cut(s) 105, 210, 276
AcoI YGGCCR 1 cut(s) 374
AcyI GRCGYC 1 cut(s) 338
AfiI CCNNNNNNNGG 1 cut(s) 369
AgsI TTSAA 1 cut(s) 64
AluBI AGCT 4 cut(s) 133, 208, 217, 399
AluI AGCT 4 cut(s) 133, 208, 217, 399
Alw26I GTCTC 2 cut(s) 42, 161
AlwI GGATC 3 cut(s) 105, 210, 276
AoxI GGCC 1 cut(s) 374
ApeKI GCWGC 2 cut(s) 89, 133
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
Asp700I GAANNNNTTC 2 cut(s) 38, 143
AspA2I CCTAGG 1 cut(s) 368
AspLEI GCGC 2 cut(s) 340, 394
AsuHPI GGTGA 2 cut(s) 22, 43
AvrII CCTAGG 1 cut(s) 368
BanI GGYRCC 1 cut(s) 337
BbvI GCAGC 2 cut(s) 76, 120
BccI CCATC 1 cut(s) 104
BcgI CGANNNNNNTGC 2 cut(s) 68, 102
BcoDI GTCTC 2 cut(s) 42, 161
BfaI CTAG 2 cut(s) 369, 409
BfoI RGCGCY 2 cut(s) 341, 395
BglI GCCNNNNNGGC 1 cut(s) 373
BisI GCNGC 3 cut(s) 90, 134, 374
BlnI CCTAGG 1 cut(s) 368
BlsI GCNGC 3 cut(s) 91, 135, 375
BmiI GGNNCC 1 cut(s) 339
BmsI GCATC 1 cut(s) 344
BsaHI GRCGYC 1 cut(s) 338
BsaI GGTCTC 1 cut(s) 161
BsaJI CCNNGG 2 cut(s) 333, 368
Bsc4I CCNNNNNNNGG 1 cut(s) 369
BseDI CCNNGG 2 cut(s) 333, 368
BseLI CCNNNNNNNGG 1 cut(s) 369
BseRI GAGGAG 1 cut(s) 302
BseX3I CGGCCG 1 cut(s) 374
BseXI GCAGC 2 cut(s) 76, 120
BseYI CCCAGC 3 cut(s) 358, 388, 395
BsgI GTGCAG 1 cut(s) 77
Bsh1285I CGRYCG 2 cut(s) 128, 377
BshFI GGCC 1 cut(s) 376
BshNI GGYRCC 1 cut(s) 337
BsiEI CGRYCG 2 cut(s) 128, 377
BsiSI CCGG 1 cut(s) 200
BslFI GGGAC 2 cut(s) 106, 274
BslI CCNNNNNNNGG 1 cut(s) 369
BsmAI GTCTC 2 cut(s) 42, 161
BsmBI CGTCTC 1 cut(s) 42
BsmFI GGGAC 2 cut(s) 106, 274
BsmI GAATGC 1 cut(s) 143
BsnI GGCC 1 cut(s) 376
Bso31I GGTCTC 1 cut(s) 161
Bsp143I GATC 5 cut(s) 97, 125, 162, 202, 268
BspACI CCGC 5 cut(s) 136, 197, 345, 365, 373
BspANI GGCC 1 cut(s) 376
BspLI GGNNCC 1 cut(s) 339
BspPI GGATC 3 cut(s) 105, 210, 276
BspQI GCTCTTC 1 cut(s) 62
BspT107I GGYRCC 1 cut(s) 337
BspTNI GGTCTC 1 cut(s) 161
BssECI CCNNGG 2 cut(s) 333, 368
BssMI GATC 5 cut(s) 97, 125, 162, 202, 268
BssNI GRCGYC 1 cut(s) 338
BssT1I CCWWGG 2 cut(s) 333, 368
Bst4CI ACNGT 2 cut(s) 31, 265
Bst6I CTCTTC 2 cut(s) 62, 290
BstACI GRCGYC 1 cut(s) 338
BstC8I GCNNGC 2 cut(s) 308, 401
BstH2I RGCGCY 2 cut(s) 341, 395
BstHHI GCGC 2 cut(s) 340, 394
BstKTI GATC 5 cut(s) 100, 128, 165, 205, 271
BstMAI GTCTC 2 cut(s) 42, 161
BstMBI GATC 5 cut(s) 97, 125, 162, 202, 268
BstMCI CGRYCG 2 cut(s) 128, 377
BstMWI GCNNNNNNNGC 5 cut(s) 205, 214, 344, 373, 400
BstV1I GCAGC 2 cut(s) 76, 120
BstX2I RGATCY 2 cut(s) 97, 268
BstYI RGATCY 2 cut(s) 97, 268
BstZI CGGCCG 1 cut(s) 374
BsuRI GGCC 1 cut(s) 376
Cac8I GCNNGC 2 cut(s) 308, 401
CfoI GCGC 2 cut(s) 340, 394
CviJI RGCY 7 cut(s) 133, 208, 217, 332, 362, 376, 399
CviKI_1 RGCY 7 cut(s) 133, 208, 217, 332, 362, 376, 399
DinI GGCGCC 1 cut(s) 339
DpnI GATC 5 cut(s) 99, 127, 164, 204, 270
DpnII GATC 5 cut(s) 97, 125, 162, 202, 268
EaeI YGGCCR 1 cut(s) 374
EagI CGGCCG 1 cut(s) 374
Eam1104I CTCTTC 2 cut(s) 62, 290
EarI CTCTTC 2 cut(s) 62, 290
EclXI CGGCCG 1 cut(s) 374
Eco130I CCWWGG 2 cut(s) 333, 368
Eco31I GGTCTC 1 cut(s) 161
Eco52I CGGCCG 1 cut(s) 374
EcoT14I CCWWGG 2 cut(s) 333, 368
EgeI GGCGCC 1 cut(s) 339
EheI GGCGCC 1 cut(s) 339
ErhI CCWWGG 2 cut(s) 333, 368
Esp3I CGTCTC 1 cut(s) 42
FaiI YATR 4 cut(s) 155, 182, 230, 304
FaqI GGGAC 2 cut(s) 106, 274
FauI CCCGC 2 cut(s) 204, 372
Fnu4HI GCNGC 3 cut(s) 90, 134, 374
Fsp4HI GCNGC 3 cut(s) 90, 134, 374
FspBI CTAG 2 cut(s) 369, 409
GlaI GCGC 2 cut(s) 339, 393
GluI GCNGC 3 cut(s) 90, 134, 374
GsaI CCCAGC 3 cut(s) 362, 392, 399
HaeII RGCGCY 2 cut(s) 341, 395
HaeIII GGCC 1 cut(s) 376
HapII CCGG 1 cut(s) 200
HhaI GCGC 2 cut(s) 340, 394
Hin1I GRCGYC 1 cut(s) 338
Hin6I GCGC 2 cut(s) 338, 392
HinP1I GCGC 2 cut(s) 338, 392
HinfI GANTC 2 cut(s) 149, 224
HpaII CCGG 1 cut(s) 200
HphI GGTGA 2 cut(s) 22, 43
Hpy166II GTNNAC 1 cut(s) 76
Hpy188I TCNGA 2 cut(s) 102, 130
Hpy188III TCNNGA 6 cut(s) 43, 64, 95, 123, 166, 272
Hpy8I GTNNAC 1 cut(s) 76
Hpy99I CGWCG 1 cut(s) 31
HpyAV CCTTC 1 cut(s) 28
HpyCH4III ACNGT 2 cut(s) 31, 265
HpyCH4V TGCA 1 cut(s) 58
HpyF10VI GCNNNNNNNGC 5 cut(s) 205, 214, 344, 373, 400
Hsp92I GRCGYC 1 cut(s) 338
HspAI GCGC 2 cut(s) 338, 392
KasI GGCGCC 1 cut(s) 337
Kzo9I GATC 5 cut(s) 97, 125, 162, 202, 268
LguI GCTCTTC 1 cut(s) 62
LmnI GCTCC 1 cut(s) 130
LpnPI CCDG 9 cut(s) 80, 213, 257, 266, 268, 292, 362, 372, 402
Lsp1109I GCAGC 2 cut(s) 76, 120
LweI GCATC 1 cut(s) 344
MaeI CTAG 2 cut(s) 369, 409
MalI GATC 5 cut(s) 99, 127, 164, 204, 270
MboI GATC 5 cut(s) 97, 125, 162, 202, 268
MboII GAAGA 2 cut(s) 79, 307
MflI RGATCY 2 cut(s) 97, 268
MluCI AATT 1 cut(s) 15
Mly113I GGCGCC 1 cut(s) 338
MlyI GAGTC 1 cut(s) 158
MnlI CCTC 3 cut(s) 46, 251, 280
MroXI GAANNNNTTC 2 cut(s) 38, 143
MslI CAYNNNNRTG 1 cut(s) 185
MspA1I CMGCKG 1 cut(s) 208
MspI CCGG 1 cut(s) 200
Mva1269I GAATGC 1 cut(s) 143
MwoI GCNNNNNNNGC 5 cut(s) 205, 214, 344, 373, 400
NarI GGCGCC 1 cut(s) 338
NdeII GATC 5 cut(s) 97, 125, 162, 202, 268
NlaIV GGNNCC 1 cut(s) 339
PciSI GCTCTTC 1 cut(s) 62
PcsI WCGNNNNNNNCGW 2 cut(s) 48, 240
PctI GAATGC 1 cut(s) 143
PdmI GAANNNNTTC 2 cut(s) 38, 143
PfeI GAWTC 1 cut(s) 224
PkrI GCNGC 3 cut(s) 91, 135, 375
Ple19I CGATCG 1 cut(s) 128
PleI GAGTC 1 cut(s) 157
PluTI GGCGCC 1 cut(s) 341
PpsI GAGTC 1 cut(s) 157
PspFI CCCAGC 3 cut(s) 358, 388, 395
PspN4I GGNNCC 1 cut(s) 339
PsuI RGATCY 2 cut(s) 97, 268
PvuI CGATCG 1 cut(s) 128
PvuII CAGCTG 1 cut(s) 208
RseI CAYNNNNRTG 1 cut(s) 185
SapI GCTCTTC 1 cut(s) 62
SatI GCNGC 3 cut(s) 90, 134, 374
Sau3AI GATC 5 cut(s) 97, 125, 162, 202, 268
SchI GAGTC 1 cut(s) 158
SetI ASST 5 cut(s) 57, 135, 210, 219, 401
SfaNI GCATC 1 cut(s) 344
SfoI GGCGCC 1 cut(s) 339
SmiMI CAYNNNNRTG 1 cut(s) 185
Sse9I AATT 1 cut(s) 15
SsiI CCGC 5 cut(s) 136, 197, 345, 365, 373
SspDI GGCGCC 1 cut(s) 337
SspMI CTAG 2 cut(s) 369, 409
StyI CCWWGG 2 cut(s) 333, 368
TaaI ACNGT 2 cut(s) 31, 265
TaqI TCGA 5 cut(s) 147, 165, 234, 243, 405
TasI AATT 1 cut(s) 15
TauI GCSGC 1 cut(s) 376
TfiI GAWTC 1 cut(s) 224
TseI GCWGC 2 cut(s) 89, 133
TspDTI ATGAA 1 cut(s) 308
TspGWI ACGGA 1 cut(s) 36
XmaJI CCTAGG 1 cut(s) 368
XmnI GAANNNNTTC 2 cut(s) 38, 143
XspI CTAG 2 cut(s) 369, 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.