Rh5DG507900

EF-hand domain pair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0004510
Physical Location & Seq
Reverse (-)
79295904 .. 79296239
336 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG507900.1

Sequence Viewer

Length: 336 bp
ATGGGAGTGGTGATAATTGACGGAACGACGGTGAAGGACTTCGTGAGCGACGAGGTGCAGTTCAAGAAGAGCGTGGACGAGCGTTTTGTTGCTCTGGATCTGAACAATGATGGTGTTCTGTCCCGGTCGGAGCTGCGGAAAGCATTCGAGTCGATGAGATTGATCGAGACCCACTTCGGCATAGAAGTGGCGACCCCGCCGGATCAGCTGACGCAGCTCTACGATTCCATATTCGATAACTTCGACTGCGACCAGAGAGGGACGGTGGATCTGGACGAGTTCAAGGAGGAGATGAAGAAGATTATGCTGGCCAAAAAAAAGATAGAAAGCGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7

Protein Analysis

111

Amino Acids

12.68

Weight (kDa)

4.6

Isoelectric Point (pI)

30.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 27 - 99 4.3e-11 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 136, 197
AclWI GGATC 3 cut(s) 105, 210, 276
AcoI YGGCCR 1 cut(s) 309
AgsI TTSAA 2 cut(s) 64, 283
AluBI AGCT 3 cut(s) 133, 208, 217
AluI AGCT 3 cut(s) 133, 208, 217
Alw26I GTCTC 1 cut(s) 161
AlwI GGATC 3 cut(s) 105, 210, 276
AoxI GGCC 1 cut(s) 309
ApeKI GCWGC 2 cut(s) 133, 214
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
Asp700I GAANNNNTTC 2 cut(s) 38, 143
AspLEI GCGC 1 cut(s) 332
AsuC2I CCSGG 1 cut(s) 124
AsuHPI GGTGA 2 cut(s) 22, 43
BalI TGGCCA 1 cut(s) 311
BbvI GCAGC 2 cut(s) 120, 226
BccI CCATC 1 cut(s) 104
BcgI CGANNNNNNTGC 2 cut(s) 132, 166
BcnI CCSGG 1 cut(s) 124
BcoDI GTCTC 1 cut(s) 161
BfaI CTAG 1 cut(s) 334
BfoI RGCGCY 1 cut(s) 333
BisI GCNGC 2 cut(s) 134, 215
BlsI GCNGC 2 cut(s) 135, 216
Bme1390I CCNGG 1 cut(s) 124
BmrFI CCNGG 1 cut(s) 124
BpuMI CCSGG 1 cut(s) 124
BsaI GGTCTC 1 cut(s) 161
BseRI GAGGAG 1 cut(s) 302
BseXI GCAGC 2 cut(s) 120, 226
BsgI GTGCAG 1 cut(s) 77
Bsh1285I CGRYCG 1 cut(s) 128
BshFI GGCC 1 cut(s) 311
BsiEI CGRYCG 1 cut(s) 128
BsiSI CCGG 2 cut(s) 124, 200
BslFI GGGAC 2 cut(s) 106, 274
BsmAI GTCTC 1 cut(s) 161
BsmFI GGGAC 2 cut(s) 106, 274
BsmI GAATGC 1 cut(s) 143
BsnI GGCC 1 cut(s) 311
Bso31I GGTCTC 1 cut(s) 161
Bsp143I GATC 4 cut(s) 97, 162, 202, 268
BspACI CCGC 2 cut(s) 136, 197
BspANI GGCC 1 cut(s) 311
BspPI GGATC 3 cut(s) 105, 210, 276
BspQI GCTCTTC 1 cut(s) 62
BspTNI GGTCTC 1 cut(s) 161
BssMI GATC 4 cut(s) 97, 162, 202, 268
Bst4CI ACNGT 2 cut(s) 31, 265
Bst6I CTCTTC 1 cut(s) 62
BstC8I GCNNGC 1 cut(s) 309
BstH2I RGCGCY 1 cut(s) 333
BstHHI GCGC 1 cut(s) 332
BstKTI GATC 4 cut(s) 100, 165, 205, 271
BstMAI GTCTC 1 cut(s) 161
BstMBI GATC 4 cut(s) 97, 162, 202, 268
BstMCI CGRYCG 1 cut(s) 128
BstMWI GCNNNNNNNGC 2 cut(s) 205, 214
BstSCI CCNGG 1 cut(s) 122
BstV1I GCAGC 2 cut(s) 120, 226
BstX2I RGATCY 2 cut(s) 97, 268
BstYI RGATCY 2 cut(s) 97, 268
BsuRI GGCC 1 cut(s) 311
Cac8I GCNNGC 1 cut(s) 309
CfoI GCGC 1 cut(s) 332
CseI GACGC 1 cut(s) 220
CviJI RGCY 4 cut(s) 133, 208, 217, 311
CviKI_1 RGCY 4 cut(s) 133, 208, 217, 311
DpnI GATC 4 cut(s) 99, 164, 204, 270
DpnII GATC 4 cut(s) 97, 162, 202, 268
EaeI YGGCCR 1 cut(s) 309
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
Eco31I GGTCTC 1 cut(s) 161
FaiI YATR 3 cut(s) 182, 230, 305
FaqI GGGAC 2 cut(s) 106, 274
FauI CCCGC 1 cut(s) 204
Fnu4HI GCNGC 2 cut(s) 134, 215
Fsp4HI GCNGC 2 cut(s) 134, 215
FspBI CTAG 1 cut(s) 334
GlaI GCGC 1 cut(s) 331
GluI GCNGC 2 cut(s) 134, 215
HaeII RGCGCY 1 cut(s) 333
HaeIII GGCC 1 cut(s) 311
HapII CCGG 2 cut(s) 124, 200
HgaI GACGC 1 cut(s) 220
HhaI GCGC 1 cut(s) 332
Hin6I GCGC 1 cut(s) 330
HinP1I GCGC 1 cut(s) 330
HinfI GANTC 2 cut(s) 149, 224
HpaII CCGG 2 cut(s) 124, 200
HphI GGTGA 2 cut(s) 22, 43
Hpy166II GTNNAC 1 cut(s) 76
Hpy188I TCNGA 2 cut(s) 102, 130
Hpy188III TCNNGA 5 cut(s) 43, 64, 95, 166, 272
Hpy8I GTNNAC 1 cut(s) 76
Hpy99I CGWCG 2 cut(s) 31, 53
HpyAV CCTTC 1 cut(s) 28
HpyCH4III ACNGT 2 cut(s) 31, 265
HpyCH4V TGCA 1 cut(s) 58
HpyF10VI GCNNNNNNNGC 2 cut(s) 205, 214
HspAI GCGC 1 cut(s) 330
Kzo9I GATC 4 cut(s) 97, 162, 202, 268
LguI GCTCTTC 1 cut(s) 62
LmnI GCTCC 1 cut(s) 130
LpnPI CCDG 6 cut(s) 80, 137, 213, 257, 266, 293
Lsp1109I GCAGC 2 cut(s) 120, 226
MaeI CTAG 1 cut(s) 334
MalI GATC 4 cut(s) 99, 164, 204, 270
MboI GATC 4 cut(s) 97, 162, 202, 268
MboII GAAGA 3 cut(s) 79, 307, 310
MflI RGATCY 2 cut(s) 97, 268
MlsI TGGCCA 1 cut(s) 311
MluCI AATT 1 cut(s) 15
MluNI TGGCCA 1 cut(s) 311
MlyI GAGTC 1 cut(s) 158
MmeI TCCRAC 1 cut(s) 108
MnlI CCTC 3 cut(s) 46, 251, 280
Mox20I TGGCCA 1 cut(s) 311
MroXI GAANNNNTTC 2 cut(s) 38, 143
MscI TGGCCA 1 cut(s) 311
MslI CAYNNNNRTG 1 cut(s) 185
Msp20I TGGCCA 1 cut(s) 311
MspA1I CMGCKG 1 cut(s) 208
MspI CCGG 2 cut(s) 124, 200
MspR9I CCNGG 1 cut(s) 124
Mva1269I GAATGC 1 cut(s) 143
MwoI GCNNNNNNNGC 2 cut(s) 205, 214
NciI CCSGG 1 cut(s) 124
NdeII GATC 4 cut(s) 97, 162, 202, 268
PciSI GCTCTTC 1 cut(s) 62
PcsI WCGNNNNNNNCGW 2 cut(s) 48, 240
PctI GAATGC 1 cut(s) 143
PdmI GAANNNNTTC 2 cut(s) 38, 143
PfeI GAWTC 1 cut(s) 224
PkrI GCNGC 2 cut(s) 135, 216
PleI GAGTC 1 cut(s) 157
PpsI GAGTC 1 cut(s) 157
PsuI RGATCY 2 cut(s) 97, 268
PvuII CAGCTG 1 cut(s) 208
RseI CAYNNNNRTG 1 cut(s) 185
SapI GCTCTTC 1 cut(s) 62
SatI GCNGC 2 cut(s) 134, 215
Sau3AI GATC 4 cut(s) 97, 162, 202, 268
SchI GAGTC 1 cut(s) 158
ScrFI CCNGG 1 cut(s) 124
SetI ASST 4 cut(s) 57, 135, 210, 219
SmiMI CAYNNNNRTG 1 cut(s) 185
Sse9I AATT 1 cut(s) 15
SsiI CCGC 2 cut(s) 136, 197
SspMI CTAG 1 cut(s) 334
StyD4I CCNGG 1 cut(s) 122
TaaI ACNGT 2 cut(s) 31, 265
TaqI TCGA 5 cut(s) 147, 152, 165, 234, 243
TasI AATT 1 cut(s) 15
TfiI GAWTC 1 cut(s) 224
TseI GCWGC 2 cut(s) 133, 214
TspDTI ATGAA 1 cut(s) 308
TspGWI ACGGA 1 cut(s) 36
XmnI GAANNNNTTC 2 cut(s) 38, 143
XspI CTAG 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.