Rroxscaffold_4G00332380

EF-hand domain pair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0004510
Physical Location & Seq
Reverse (-)
66514314 .. 66515133
820 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00332380.1

Sequence Viewer

Length: 435 bp
ATGGGAGTGGTGATAATTGACGGAACGACGGTGAAGAACTTCGTGAGCGACGAGGTGCAGTTCAAGAAGAGCGTGGACGAGCGTTTTGCTGCTCTGGATCTGAACAACGATGGTGTTCTGTCCCGGTCGGAGCTGCGGAAAGCATTCGAGTCGATGAGATTGATCGAGACCCACTTCGGCATAGACGTGGCGACCTCGCCGGATCAGCTGACGCAGCTCTACGATTCCATATTCGATAAGTTCGACTGTGACCAGAGCGGGACGGTGGATCTGGACGAGTTCAGGGAGGAGATGAAGAAGATTATGCTGGCCATCGCCGACGGGCTTGGCTCTTGCCCTATTCAGATGGCTCTCGAGGACGACCACCACAGCCTTCTCCAGCAGGCTGCAGATCTCGAGGCTTCCAAGATATCATCATCAGTTTCACACACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7

Protein Analysis

144

Amino Acids

15.97

Weight (kDa)

4.44

Isoelectric Point (pI)

35.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 27 - 99 9.3e-12 EF-hand domain pair
EF-hand_1 PF00036 75 - 100 3.3e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 258
AciI CCGC 2 cut(s) 136, 258
AclWI GGATC 3 cut(s) 105, 210, 276
AcoI YGGCCR 1 cut(s) 309
AgsI TTSAA 1 cut(s) 64
AjiI CACGTC 1 cut(s) 187
AluBI AGCT 3 cut(s) 133, 208, 217
AluI AGCT 3 cut(s) 133, 208, 217
Alw26I GTCTC 1 cut(s) 161
AlwI GGATC 3 cut(s) 105, 210, 276
Ama87I CYCGRG 2 cut(s) 353, 395
AoxI GGCC 1 cut(s) 309
ApeKI GCWGC 4 cut(s) 89, 133, 214, 386
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
Asp700I GAANNNNTTC 2 cut(s) 38, 143
AsuC2I CCSGG 1 cut(s) 124
AsuHPI GGTGA 2 cut(s) 22, 43
AvaI CYCGRG 2 cut(s) 353, 395
BalI TGGCCA 1 cut(s) 311
BbvI GCAGC 4 cut(s) 76, 120, 226, 373
BccI CCATC 3 cut(s) 104, 320, 340
BcgI CGANNNNNNTGC 4 cut(s) 68, 102, 132, 166
BcnI CCSGG 1 cut(s) 124
BcoDI GTCTC 1 cut(s) 161
BfaI CTAG 1 cut(s) 433
BfmI CTRYAG 1 cut(s) 387
BglII AGATCT 1 cut(s) 391
BisI GCNGC 4 cut(s) 90, 134, 215, 387
BlsI GCNGC 4 cut(s) 91, 135, 216, 388
Bme1390I CCNGG 1 cut(s) 124
BmeT110I CYCGRG 2 cut(s) 353, 395
BmgBI CACGTC 1 cut(s) 187
BmrFI CCNGG 1 cut(s) 124
BpmI CTGGAG 1 cut(s) 362
BpuMI CCSGG 1 cut(s) 124
BsaI GGTCTC 1 cut(s) 161
BseRI GAGGAG 1 cut(s) 302
BseXI GCAGC 4 cut(s) 76, 120, 226, 373
BsgI GTGCAG 1 cut(s) 77
Bsh1285I CGRYCG 1 cut(s) 128
BshFI GGCC 1 cut(s) 311
BsiEI CGRYCG 1 cut(s) 128
BsiHKCI CYCGRG 2 cut(s) 353, 395
BsiSI CCGG 2 cut(s) 124, 200
BslFI GGGAC 2 cut(s) 106, 274
BsmAI GTCTC 1 cut(s) 161
BsmFI GGGAC 2 cut(s) 106, 274
BsmI GAATGC 1 cut(s) 143
BsnI GGCC 1 cut(s) 311
Bso31I GGTCTC 1 cut(s) 161
BsoBI CYCGRG 2 cut(s) 353, 395
Bsp143I GATC 5 cut(s) 97, 162, 202, 268, 391
BspACI CCGC 2 cut(s) 136, 258
BspANI GGCC 1 cut(s) 311
BspMAI CTGCAG 1 cut(s) 391
BspPI GGATC 3 cut(s) 105, 210, 276
BspQI GCTCTTC 1 cut(s) 62
BspTNI GGTCTC 1 cut(s) 161
BsrBI CCGCTC 1 cut(s) 258
BssMI GATC 5 cut(s) 97, 162, 202, 268, 391
Bst4CI ACNGT 3 cut(s) 31, 248, 265
Bst6I CTCTTC 1 cut(s) 62
BstC8I GCNNGC 2 cut(s) 309, 384
BstKTI GATC 5 cut(s) 100, 165, 205, 271, 394
BstMAI GTCTC 1 cut(s) 161
BstMBI GATC 5 cut(s) 97, 162, 202, 268, 391
BstMCI CGRYCG 1 cut(s) 128
BstMWI GCNNNNNNNGC 2 cut(s) 205, 214
BstSCI CCNGG 1 cut(s) 122
BstSFI CTRYAG 1 cut(s) 387
BstV1I GCAGC 4 cut(s) 76, 120, 226, 373
BstX2I RGATCY 3 cut(s) 97, 268, 391
BstYI RGATCY 3 cut(s) 97, 268, 391
BsuRI GGCC 1 cut(s) 311
BtgZI GCGATG 1 cut(s) 298
BtrI CACGTC 1 cut(s) 187
Cac8I GCNNGC 2 cut(s) 309, 384
CseI GACGC 1 cut(s) 220
DpnI GATC 5 cut(s) 99, 164, 204, 270, 393
DpnII GATC 5 cut(s) 97, 162, 202, 268, 391
EaeI YGGCCR 1 cut(s) 309
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
Eco31I GGTCTC 1 cut(s) 161
Eco32I GATATC 1 cut(s) 411
Eco88I CYCGRG 2 cut(s) 353, 395
EcoRV GATATC 1 cut(s) 411
FaiI YATR 3 cut(s) 182, 230, 305
FaqI GGGAC 2 cut(s) 106, 274
FauI CCCGC 1 cut(s) 251
Fnu4HI GCNGC 4 cut(s) 90, 134, 215, 387
Fsp4HI GCNGC 4 cut(s) 90, 134, 215, 387
FspBI CTAG 1 cut(s) 433
GluI GCNGC 4 cut(s) 90, 134, 215, 387
GsuI CTGGAG 1 cut(s) 362
HaeIII GGCC 1 cut(s) 311
HapII CCGG 2 cut(s) 124, 200
HgaI GACGC 1 cut(s) 220
HinfI GANTC 2 cut(s) 149, 224
HpaII CCGG 2 cut(s) 124, 200
HphI GGTGA 2 cut(s) 22, 43
Hpy166II GTNNAC 1 cut(s) 76
Hpy188I TCNGA 3 cut(s) 102, 130, 345
Hpy188III TCNNGA 7 cut(s) 43, 64, 95, 166, 272, 353, 395
Hpy8I GTNNAC 1 cut(s) 76
Hpy99I CGWCG 3 cut(s) 31, 53, 323
HpyAV CCTTC 1 cut(s) 383
HpyCH4III ACNGT 3 cut(s) 31, 248, 265
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 2 cut(s) 58, 389
HpyF10VI GCNNNNNNNGC 2 cut(s) 205, 214
HpySE526I ACGT 1 cut(s) 186
Kzo9I GATC 5 cut(s) 97, 162, 202, 268, 391
LguI GCTCTTC 1 cut(s) 62
LmnI GCTCC 1 cut(s) 130
LpnPI CCDG 9 cut(s) 80, 137, 213, 257, 266, 268, 293, 368, 392
Lsp1109I GCAGC 4 cut(s) 76, 120, 226, 373
MaeI CTAG 1 cut(s) 433
MaeII ACGT 1 cut(s) 186
MaeIII GTNAC 1 cut(s) 248
MalI GATC 5 cut(s) 99, 164, 204, 270, 393
MbiI CCGCTC 1 cut(s) 258
MboI GATC 5 cut(s) 97, 162, 202, 268, 391
MboII GAAGA 4 cut(s) 46, 79, 307, 310
MflI RGATCY 3 cut(s) 97, 268, 391
MlsI TGGCCA 1 cut(s) 311
MluCI AATT 1 cut(s) 15
MluNI TGGCCA 1 cut(s) 311
MlyI GAGTC 1 cut(s) 158
MmeI TCCRAC 1 cut(s) 108
MnlI CCTC 5 cut(s) 46, 205, 280, 349, 391
Mox20I TGGCCA 1 cut(s) 311
MroXI GAANNNNTTC 2 cut(s) 38, 143
MscI TGGCCA 1 cut(s) 311
MslI CAYNNNNRTG 1 cut(s) 185
Msp20I TGGCCA 1 cut(s) 311
MspA1I CMGCKG 1 cut(s) 208
MspI CCGG 2 cut(s) 124, 200
MspR9I CCNGG 1 cut(s) 124
Mva1269I GAATGC 1 cut(s) 143
MwoI GCNNNNNNNGC 2 cut(s) 205, 214
NciI CCSGG 1 cut(s) 124
NdeII GATC 5 cut(s) 97, 162, 202, 268, 391
NmuCI GTSAC 1 cut(s) 248
PaeR7I CTCGAG 2 cut(s) 353, 395
PciSI GCTCTTC 1 cut(s) 62
PcsI WCGNNNNNNNCGW 3 cut(s) 48, 183, 240
PctI GAATGC 1 cut(s) 143
PdmI GAANNNNTTC 2 cut(s) 38, 143
PfeI GAWTC 1 cut(s) 224
PkrI GCNGC 4 cut(s) 91, 135, 216, 388
PleI GAGTC 1 cut(s) 157
PpsI GAGTC 1 cut(s) 157
PstI CTGCAG 1 cut(s) 391
PsuI RGATCY 3 cut(s) 97, 268, 391
PvuII CAGCTG 1 cut(s) 208
RseI CAYNNNNRTG 1 cut(s) 185
SapI GCTCTTC 1 cut(s) 62
SatI GCNGC 4 cut(s) 90, 134, 215, 387
Sau3AI GATC 5 cut(s) 97, 162, 202, 268, 391
SchI GAGTC 1 cut(s) 158
ScrFI CCNGG 1 cut(s) 124
SetI ASST 7 cut(s) 57, 135, 189, 197, 210, 219, 434
SfcI CTRYAG 1 cut(s) 387
Sfr274I CTCGAG 2 cut(s) 353, 395
SlaI CTCGAG 2 cut(s) 353, 395
SmiMI CAYNNNNRTG 1 cut(s) 185
SmlI CTYRAG 2 cut(s) 353, 395
SmoI CTYRAG 2 cut(s) 353, 395
Sse9I AATT 1 cut(s) 15
SsiI CCGC 2 cut(s) 136, 258
SspMI CTAG 1 cut(s) 433
StyD4I CCNGG 1 cut(s) 122
TaaI ACNGT 3 cut(s) 31, 248, 265
TaiI ACGT 1 cut(s) 189
TaqI TCGA 7 cut(s) 147, 152, 165, 234, 243, 354, 396
TasI AATT 1 cut(s) 15
TfiI GAWTC 1 cut(s) 224
TseFI GTSAC 1 cut(s) 248
TseI GCWGC 4 cut(s) 89, 133, 214, 386
Tsp45I GTSAC 1 cut(s) 248
TspDTI ATGAA 1 cut(s) 308
TspGWI ACGGA 1 cut(s) 36
XhoI CTCGAG 2 cut(s) 353, 395
XmnI GAANNNNTTC 2 cut(s) 38, 143
XspI CTAG 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.