RchiOBHm_Chr4g0404011

EF-hand domain pair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0004510
Physical Location & Seq
Reverse (-)
23523232 .. 23523555
324 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37564

Sequence Viewer

Length: 324 bp
ATGGGAGTGGTGATAATTGACGGAACGACGGTGAAGGACTTCGTGAGTGACGAGGTGCAGTTCAAGAAGAGCGTGGACGAGCGTTTTCCTGCTCTGGATCTGAACAACGACGGTGTTCTGTCCCGGTCGGAGCTGCGGAAAGCATTCGAGTCGATGAGATTCATCGAGACCCACTTCGGCATAGAAGTGGCGACCCCGCCGGATCAGCTGACGCAGCTCTACGATTCCATATTCGATAAGTTCGACTGCGACCAGAGAGGAACGGTGGATCTGGACGAGTTCAGGGAGGAGATGAAGAGATTATGCTGGCCAAAAAAAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7

Protein Analysis

107

Amino Acids

12.48

Weight (kDa)

4.77

Isoelectric Point (pI)

41.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 27 - 99 4.4e-11 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 136, 197
AclWI GGATC 3 cut(s) 105, 210, 276
AcoI YGGCCR 1 cut(s) 308
AgsI TTSAA 1 cut(s) 64
AluBI AGCT 3 cut(s) 133, 208, 217
AluI AGCT 3 cut(s) 133, 208, 217
Alw26I GTCTC 1 cut(s) 161
AlwI GGATC 3 cut(s) 105, 210, 276
AoxI GGCC 1 cut(s) 308
ApeKI GCWGC 2 cut(s) 133, 214
Asp700I GAANNNNTTC 2 cut(s) 38, 143
AsuC2I CCSGG 1 cut(s) 124
AsuHPI GGTGA 2 cut(s) 22, 43
BalI TGGCCA 1 cut(s) 310
BbvI GCAGC 2 cut(s) 120, 226
BcgI CGANNNNNNTGC 2 cut(s) 132, 166
BcnI CCSGG 1 cut(s) 124
BcoDI GTCTC 1 cut(s) 161
BisI GCNGC 2 cut(s) 134, 215
BlsI GCNGC 2 cut(s) 135, 216
Bme1390I CCNGG 1 cut(s) 124
BmrFI CCNGG 1 cut(s) 124
BpuMI CCSGG 1 cut(s) 124
BsaI GGTCTC 1 cut(s) 161
BseRI GAGGAG 1 cut(s) 302
BseXI GCAGC 2 cut(s) 120, 226
BsgI GTGCAG 1 cut(s) 77
Bsh1285I CGRYCG 1 cut(s) 128
BshFI GGCC 1 cut(s) 310
BsiEI CGRYCG 1 cut(s) 128
BsiSI CCGG 2 cut(s) 124, 200
BslFI GGGAC 1 cut(s) 106
BsmAI GTCTC 1 cut(s) 161
BsmFI GGGAC 1 cut(s) 106
BsmI GAATGC 1 cut(s) 143
BsnI GGCC 1 cut(s) 310
Bso31I GGTCTC 1 cut(s) 161
Bsp143I GATC 3 cut(s) 97, 202, 268
BspACI CCGC 2 cut(s) 136, 197
BspANI GGCC 1 cut(s) 310
BspPI GGATC 3 cut(s) 105, 210, 276
BspQI GCTCTTC 1 cut(s) 62
BspTNI GGTCTC 1 cut(s) 161
BssMI GATC 3 cut(s) 97, 202, 268
Bst4CI ACNGT 3 cut(s) 31, 113, 265
Bst6I CTCTTC 2 cut(s) 62, 290
BstC8I GCNNGC 1 cut(s) 308
BstKTI GATC 3 cut(s) 100, 205, 271
BstMAI GTCTC 1 cut(s) 161
BstMBI GATC 3 cut(s) 97, 202, 268
BstMCI CGRYCG 1 cut(s) 128
BstMWI GCNNNNNNNGC 2 cut(s) 205, 214
BstSCI CCNGG 1 cut(s) 122
BstV1I GCAGC 2 cut(s) 120, 226
BstX2I RGATCY 2 cut(s) 97, 268
BstYI RGATCY 2 cut(s) 97, 268
BsuRI GGCC 1 cut(s) 310
Cac8I GCNNGC 1 cut(s) 308
CseI GACGC 1 cut(s) 220
CviJI RGCY 4 cut(s) 133, 208, 217, 310
CviKI_1 RGCY 4 cut(s) 133, 208, 217, 310
DpnI GATC 3 cut(s) 99, 204, 270
DpnII GATC 3 cut(s) 97, 202, 268
EaeI YGGCCR 1 cut(s) 308
Eam1104I CTCTTC 2 cut(s) 62, 290
EarI CTCTTC 2 cut(s) 62, 290
Eco31I GGTCTC 1 cut(s) 161
FaiI YATR 3 cut(s) 182, 230, 304
FaqI GGGAC 1 cut(s) 106
FauI CCCGC 1 cut(s) 204
Fnu4HI GCNGC 2 cut(s) 134, 215
Fsp4HI GCNGC 2 cut(s) 134, 215
GluI GCNGC 2 cut(s) 134, 215
HaeIII GGCC 1 cut(s) 310
HapII CCGG 2 cut(s) 124, 200
HgaI GACGC 1 cut(s) 220
HinfI GANTC 3 cut(s) 149, 159, 224
HpaII CCGG 2 cut(s) 124, 200
HphI GGTGA 2 cut(s) 22, 43
Hpy166II GTNNAC 1 cut(s) 76
Hpy188I TCNGA 2 cut(s) 102, 130
Hpy188III TCNNGA 5 cut(s) 43, 64, 95, 166, 272
Hpy8I GTNNAC 1 cut(s) 76
Hpy99I CGWCG 2 cut(s) 31, 113
HpyAV CCTTC 1 cut(s) 28
HpyCH4III ACNGT 3 cut(s) 31, 113, 265
HpyCH4V TGCA 1 cut(s) 58
HpyF10VI GCNNNNNNNGC 2 cut(s) 205, 214
Kzo9I GATC 3 cut(s) 97, 202, 268
LguI GCTCTTC 1 cut(s) 62
LmnI GCTCC 1 cut(s) 130
LpnPI CCDG 8 cut(s) 80, 102, 137, 213, 257, 266, 268, 292
Lsp1109I GCAGC 2 cut(s) 120, 226
MaeIII GTNAC 1 cut(s) 47
MalI GATC 3 cut(s) 99, 204, 270
MboI GATC 3 cut(s) 97, 202, 268
MboII GAAGA 2 cut(s) 79, 307
MflI RGATCY 2 cut(s) 97, 268
MlsI TGGCCA 1 cut(s) 310
MluCI AATT 1 cut(s) 15
MluNI TGGCCA 1 cut(s) 310
MlyI GAGTC 1 cut(s) 158
MmeI TCCRAC 1 cut(s) 108
MnlI CCTC 3 cut(s) 46, 251, 280
Mox20I TGGCCA 1 cut(s) 310
MroXI GAANNNNTTC 2 cut(s) 38, 143
MscI TGGCCA 1 cut(s) 310
MslI CAYNNNNRTG 1 cut(s) 185
Msp20I TGGCCA 1 cut(s) 310
MspA1I CMGCKG 1 cut(s) 208
MspI CCGG 2 cut(s) 124, 200
MspR9I CCNGG 1 cut(s) 124
Mva1269I GAATGC 1 cut(s) 143
MwoI GCNNNNNNNGC 2 cut(s) 205, 214
NciI CCSGG 1 cut(s) 124
NdeII GATC 3 cut(s) 97, 202, 268
NmuCI GTSAC 1 cut(s) 47
PciSI GCTCTTC 1 cut(s) 62
PcsI WCGNNNNNNNCGW 2 cut(s) 48, 240
PctI GAATGC 1 cut(s) 143
PdmI GAANNNNTTC 2 cut(s) 38, 143
PfeI GAWTC 2 cut(s) 159, 224
PkrI GCNGC 2 cut(s) 135, 216
PleI GAGTC 1 cut(s) 157
PpsI GAGTC 1 cut(s) 157
PsuI RGATCY 2 cut(s) 97, 268
PvuII CAGCTG 1 cut(s) 208
RseI CAYNNNNRTG 1 cut(s) 185
SapI GCTCTTC 1 cut(s) 62
SatI GCNGC 2 cut(s) 134, 215
Sau3AI GATC 3 cut(s) 97, 202, 268
SchI GAGTC 1 cut(s) 158
ScrFI CCNGG 1 cut(s) 124
SetI ASST 4 cut(s) 57, 135, 210, 219
SmiMI CAYNNNNRTG 1 cut(s) 185
Sse9I AATT 1 cut(s) 15
SsiI CCGC 2 cut(s) 136, 197
StyD4I CCNGG 1 cut(s) 122
TaaI ACNGT 3 cut(s) 31, 113, 265
TaqI TCGA 5 cut(s) 147, 152, 165, 234, 243
TasI AATT 1 cut(s) 15
TfiI GAWTC 2 cut(s) 159, 224
TseFI GTSAC 1 cut(s) 47
TseI GCWGC 2 cut(s) 133, 214
Tsp45I GTSAC 1 cut(s) 47
TspDTI ATGAA 2 cut(s) 151, 308
TspGWI ACGGA 1 cut(s) 36
XmnI GAANNNNTTC 2 cut(s) 38, 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.