Rmu_sc0003005.1_g000020

EF-hand domain pair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003005.1
OG0004510
Physical Location & Seq
Forward (+)
91357 .. 91749
393 bp
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UTR
Exon/CDS
Intron
Rmu_sc0003005.1_g000020.1.cds

Sequence Viewer

Length: 393 bp
atgttggcagacatttattggcagacatttacgcaggaaaagaagaaaagaaaaatcgacggaggaaagatgggagtggtgataattgacggaacgacggtgaaggacttcgtgagcgacgaggtgcagttcaagaagagcgtggacgagcgttttgctgctctggatctgaacaacgatggtgttctgtcccggtcggagctgcggaaagcatttgagtcgatgagattgatcgagacccacttcgacatagaagtggcgaccccgccggatcagctgacacagctctaccattccatattcgataagtttgactgcgaccagagagggacggtggatctggacgagttcagggaggagatgaagagattatgttggcaaaaaaaaagatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7

Protein Analysis

130

Amino Acids

15.3

Weight (kDa)

5.45

Isoelectric Point (pI)

37.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 205, 266
AclWI GGATC 3 cut(s) 174, 279, 345
AgsI TTSAA 1 cut(s) 133
AluBI AGCT 3 cut(s) 202, 277, 286
AluI AGCT 3 cut(s) 202, 277, 286
Alw26I GTCTC 1 cut(s) 230
AlwI GGATC 3 cut(s) 174, 279, 345
ApeKI GCWGC 2 cut(s) 158, 202
ArsI GACNNNNNNTTYG 2 cut(s) 137, 169
Asp700I GAANNNNTTC 1 cut(s) 107
AsuC2I CCSGG 1 cut(s) 193
AsuHPI GGTGA 2 cut(s) 91, 112
BbvI GCAGC 2 cut(s) 145, 189
BccI CCATC 2 cut(s) 64, 173
BcgI CGANNNNNNTGC 4 cut(s) 137, 171, 201, 235
BcnI CCSGG 1 cut(s) 193
BcoDI GTCTC 1 cut(s) 230
BisI GCNGC 2 cut(s) 159, 203
BlsI GCNGC 2 cut(s) 160, 204
Bme1390I CCNGG 1 cut(s) 193
BmrFI CCNGG 1 cut(s) 193
BpuMI CCSGG 1 cut(s) 193
BsaI GGTCTC 1 cut(s) 230
BseRI GAGGAG 1 cut(s) 371
BseXI GCAGC 2 cut(s) 145, 189
BsgI GTGCAG 1 cut(s) 146
Bsh1285I CGRYCG 1 cut(s) 197
BsiEI CGRYCG 1 cut(s) 197
BsiSI CCGG 2 cut(s) 193, 269
BslFI GGGAC 2 cut(s) 175, 343
BsmAI GTCTC 1 cut(s) 230
BsmFI GGGAC 2 cut(s) 175, 343
Bso31I GGTCTC 1 cut(s) 230
Bsp143I GATC 4 cut(s) 166, 231, 271, 337
BspACI CCGC 2 cut(s) 205, 266
BspPI GGATC 3 cut(s) 174, 279, 345
BspQI GCTCTTC 1 cut(s) 131
BspTNI GGTCTC 1 cut(s) 230
BssMI GATC 4 cut(s) 166, 231, 271, 337
Bst4CI ACNGT 2 cut(s) 100, 334
Bst6I CTCTTC 2 cut(s) 131, 359
BstKTI GATC 4 cut(s) 169, 234, 274, 340
BstMAI GTCTC 1 cut(s) 230
BstMBI GATC 4 cut(s) 166, 231, 271, 337
BstMCI CGRYCG 1 cut(s) 197
BstMWI GCNNNNNNNGC 2 cut(s) 274, 283
BstSCI CCNGG 1 cut(s) 191
BstV1I GCAGC 2 cut(s) 145, 189
BstX2I RGATCY 2 cut(s) 166, 337
BstYI RGATCY 2 cut(s) 166, 337
CviJI RGCY 3 cut(s) 202, 277, 286
CviKI_1 RGCY 3 cut(s) 202, 277, 286
DpnI GATC 4 cut(s) 168, 233, 273, 339
DpnII GATC 4 cut(s) 166, 231, 271, 337
Eam1104I CTCTTC 2 cut(s) 131, 359
EarI CTCTTC 2 cut(s) 131, 359
Eco31I GGTCTC 1 cut(s) 230
FaiI YATR 3 cut(s) 251, 299, 373
FaqI GGGAC 2 cut(s) 175, 343
FauI CCCGC 1 cut(s) 273
Fnu4HI GCNGC 2 cut(s) 159, 203
Fsp4HI GCNGC 2 cut(s) 159, 203
GluI GCNGC 2 cut(s) 159, 203
HapII CCGG 2 cut(s) 193, 269
HinfI GANTC 1 cut(s) 218
HpaII CCGG 2 cut(s) 193, 269
HphI GGTGA 2 cut(s) 91, 112
Hpy166II GTNNAC 1 cut(s) 145
Hpy188I TCNGA 2 cut(s) 171, 199
Hpy188III TCNNGA 5 cut(s) 112, 133, 164, 235, 341
Hpy8I GTNNAC 1 cut(s) 145
Hpy99I CGWCG 3 cut(s) 62, 100, 122
HpyAV CCTTC 1 cut(s) 97
HpyCH4III ACNGT 2 cut(s) 100, 334
HpyCH4V TGCA 1 cut(s) 127
HpyF10VI GCNNNNNNNGC 2 cut(s) 274, 283
Kzo9I GATC 4 cut(s) 166, 231, 271, 337
LguI GCTCTTC 1 cut(s) 131
LmnI GCTCC 1 cut(s) 199
LpnPI CCDG 7 cut(s) 20, 149, 206, 282, 326, 335, 337
Lsp1109I GCAGC 2 cut(s) 145, 189
MalI GATC 4 cut(s) 168, 233, 273, 339
MboI GATC 4 cut(s) 166, 231, 271, 337
MboII GAAGA 3 cut(s) 55, 148, 376
MflI RGATCY 2 cut(s) 166, 337
MluCI AATT 1 cut(s) 84
MlyI GAGTC 1 cut(s) 227
MmeI TCCRAC 1 cut(s) 177
MnlI CCTC 4 cut(s) 56, 115, 320, 349
MroXI GAANNNNTTC 1 cut(s) 107
MslI CAYNNNNRTG 1 cut(s) 254
MspA1I CMGCKG 1 cut(s) 277
MspI CCGG 2 cut(s) 193, 269
MspR9I CCNGG 1 cut(s) 193
MwoI GCNNNNNNNGC 2 cut(s) 274, 283
NciI CCSGG 1 cut(s) 193
NdeII GATC 4 cut(s) 166, 231, 271, 337
PciSI GCTCTTC 1 cut(s) 131
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PdmI GAANNNNTTC 1 cut(s) 107
PkrI GCNGC 2 cut(s) 160, 204
PleI GAGTC 1 cut(s) 226
PpsI GAGTC 1 cut(s) 226
PsuI RGATCY 2 cut(s) 166, 337
PvuII CAGCTG 1 cut(s) 277
RseI CAYNNNNRTG 1 cut(s) 254
SapI GCTCTTC 1 cut(s) 131
SatI GCNGC 2 cut(s) 159, 203
Sau3AI GATC 4 cut(s) 166, 231, 271, 337
SchI GAGTC 1 cut(s) 227
ScrFI CCNGG 1 cut(s) 193
SetI ASST 4 cut(s) 126, 204, 279, 288
SmiMI CAYNNNNRTG 1 cut(s) 254
Sse9I AATT 1 cut(s) 84
SsiI CCGC 2 cut(s) 205, 266
StyD4I CCNGG 1 cut(s) 191
TaaI ACNGT 2 cut(s) 100, 334
TaqI TCGA 5 cut(s) 57, 221, 234, 246, 303
TasI AATT 1 cut(s) 84
TseI GCWGC 2 cut(s) 158, 202
TspDTI ATGAA 1 cut(s) 377
TspGWI ACGGA 2 cut(s) 75, 105
XmnI GAANNNNTTC 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.