FvH4_7g01830

Triphosphate tunel metalloenzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0002663
Physical Location & Seq
Forward (+)
2193399 .. 2194022
624 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g01830.t1

Sequence Viewer

Length: 624 bp
ATGTCTCACGTCTCCAAAGCCCTAATGGAGGTCGAGGTCAAGCTGCGGCTGCCGGACGCCGCCGCGCACGGCCAGGTCACCACTCTTTTAGCGCCGTTCCACCGCGGGACCCACCGCCAGGAGAATCTCTTCTTCGACGGCTCCAAATCCGAGCTCTCGGCGCGCCGGGCCGTGCTCCGGCTCCGGTTCTCCGACCGGAGCCCTCTCTGCGCGGTGACGCTGAAGGCGAAGGCGGTGCTCGTCGACGGCGTCAGCCGAGTGGAGGAGGACGAGGAGGAGATGGACCCGGCGGTGGGGCGGGCGTGCGCCGCCGAGCCGGAGAAGCTGATGGCGGCGGAGTCTAGGGTTTTGAGAAGGGTGAGGGAGGAGTTTGGGGTTTTAGGGTTTGTGGGGATGGGAGGGTTTGGGAATGTGAGGGATGTGTATGATTGGAAAGGGTTGAAGTTGGAGGTGGATGAGACTAAGTATGAGTTTGGGACTTGTTATGAGATTGAGTGTGAGAGTGCTGATCCTGATGGAGCTAAGGAGAAGCTTGAGGAGTTTTTGAAGGAAAATGGGGTGAGGTATTCCTACTCCCAAGTCTCCAAGTTTGCAACTTTTCGAGCTGGGAAGCTGCCTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CYTH

Protein Analysis

208

Amino Acids

23.11

Weight (kDa)

5.72

Isoelectric Point (pI)

44.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CYTH PF01928 9 - 186 2.1e-19 CYTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 243
AccII CGCG 4 cut(s) 65, 105, 163, 212
AclWI GGATC 1 cut(s) 503
AcoI YGGCCR 1 cut(s) 70
AcuI CTGAAG 1 cut(s) 242
AcyI GRCGYC 2 cut(s) 57, 249
AfiI CCNNNNNNNGG 6 cut(s) 28, 118, 177, 262, 292, 293
AgsI TTSAA 2 cut(s) 442, 547
AjiI CACGTC 1 cut(s) 10
AjnI CCWGG 2 cut(s) 72, 117
AluBI AGCT 7 cut(s) 43, 154, 325, 521, 532, 605, 613
AluI AGCT 7 cut(s) 43, 154, 325, 521, 532, 605, 613
Alw21I GWGCWC 3 cut(s) 156, 177, 240
Alw26I GTCTC 4 cut(s) 9, 16, 452, 586
AlwI GGATC 1 cut(s) 503
AoxI GGCC 2 cut(s) 70, 168
ApeKI GCWGC 3 cut(s) 43, 49, 613
AscI GGCGCGCC 1 cut(s) 161
Asp700I GAANNNNTTC 1 cut(s) 128
AspLEI GCGC 6 cut(s) 67, 94, 163, 165, 212, 308
AspS9I GGNCC 3 cut(s) 108, 168, 283
AsuC2I CCSGG 2 cut(s) 167, 287
AsuHPI GGTGA 4 cut(s) 70, 226, 370, 571
AvaII GGWCC 2 cut(s) 108, 283
BanII GRGCYC 2 cut(s) 156, 203
Bbv12I GWGCWC 3 cut(s) 156, 177, 240
BbvI GCAGC 3 cut(s) 30, 36, 600
BccI CCATC 4 cut(s) 274, 322, 388, 509
BceAI ACGGC 5 cut(s) 79, 85, 154, 155, 262
BcgI CGANNNNNNTGC 2 cut(s) 217, 251
BciT130I CCWGG 2 cut(s) 74, 119
BcnI CCSGG 2 cut(s) 167, 287
BcoDI GTCTC 4 cut(s) 9, 16, 452, 586
BfaI CTAG 1 cut(s) 342
BfoI RGCGCY 1 cut(s) 95
BisI GCNGC 8 cut(s) 44, 47, 50, 60, 63, 309, 333, 614
BlsI GCNGC 8 cut(s) 45, 48, 51, 61, 64, 310, 334, 615
Bme1390I CCNGG 4 cut(s) 74, 119, 167, 287
Bme18I GGWCC 2 cut(s) 108, 283
BmgBI CACGTC 1 cut(s) 10
BmgT120I GGNCC 3 cut(s) 108, 168, 283
BmiI GGNNCC 6 cut(s) 109, 110, 142, 182, 200, 285
BmrFI CCNGG 4 cut(s) 74, 119, 167, 287
Bpu10I CCTNAGC 1 cut(s) 522
BpuEI CTTGAG 1 cut(s) 554
BpuMI CCSGG 2 cut(s) 167, 287
BsaHI GRCGYC 2 cut(s) 57, 249
BsaJI CCNNGG 1 cut(s) 103
BsaWI WCCGGW 2 cut(s) 183, 195
Bsc4I CCNNNNNNNGG 6 cut(s) 28, 118, 177, 262, 292, 293
BseBI CCWGG 2 cut(s) 74, 119
BseDI CCNNGG 1 cut(s) 103
BseGI GGATG 3 cut(s) 399, 424, 460
BseLI CCNNNNNNNGG 6 cut(s) 28, 118, 177, 262, 292, 293
BsePI GCGCGC 1 cut(s) 161
BseRI GAGGAG 5 cut(s) 278, 287, 290, 380, 551
BseXI GCAGC 3 cut(s) 30, 36, 600
BseYI CCCAGC 1 cut(s) 605
Bsh1236I CGCG 4 cut(s) 65, 105, 163, 212
Bsh1285I CGRYCG 1 cut(s) 196
BshFI GGCC 2 cut(s) 72, 170
BsiEI CGRYCG 1 cut(s) 196
BsiHKAI GWGCWC 3 cut(s) 156, 177, 240
BsiSI CCGG 7 cut(s) 53, 166, 178, 184, 196, 287, 317
BslFI GGGAC 2 cut(s) 121, 490
BslI CCNNNNNNNGG 6 cut(s) 28, 118, 177, 262, 292, 293
BsmAI GTCTC 4 cut(s) 9, 16, 452, 586
BsmBI CGTCTC 1 cut(s) 16
BsmFI GGGAC 2 cut(s) 121, 490
BsnI GGCC 2 cut(s) 72, 170
Bsp1286I GDGCHC 4 cut(s) 156, 177, 203, 240
Bsp143I GATC 1 cut(s) 508
BspANI GGCC 2 cut(s) 72, 170
BspFNI CGCG 4 cut(s) 65, 105, 163, 212
BspLI GGNNCC 6 cut(s) 109, 110, 142, 182, 200, 285
BspPI GGATC 1 cut(s) 503
BssECI CCNNGG 1 cut(s) 103
BssHII GCGCGC 1 cut(s) 161
BssMI GATC 1 cut(s) 508
BssNI GRCGYC 2 cut(s) 57, 249
Bst2UI CCWGG 2 cut(s) 74, 119
Bst6I CTCTTC 1 cut(s) 134
BstACI GRCGYC 2 cut(s) 57, 249
BstC8I GCNNGC 3 cut(s) 163, 300, 304
BstDEI CTNAG 2 cut(s) 462, 522
BstDSI CCRYGG 1 cut(s) 103
BstEII GGTNACC 1 cut(s) 76
BstENI CCTNNNNNAGG 1 cut(s) 26
BstF5I GGATG 3 cut(s) 399, 424, 460
BstFNI CGCG 4 cut(s) 65, 105, 163, 212
BstH2I RGCGCY 1 cut(s) 95
BstHHI GCGC 6 cut(s) 67, 94, 163, 165, 212, 308
BstKTI GATC 1 cut(s) 511
BstMAI GTCTC 4 cut(s) 9, 16, 452, 586
BstMBI GATC 1 cut(s) 508
BstMCI CGRYCG 1 cut(s) 196
BstMWI GCNNNNNNNGC 6 cut(s) 49, 160, 167, 207, 308, 322
BstNI CCWGG 2 cut(s) 74, 119
BstPI GGTNACC 1 cut(s) 76
BstSCI CCNGG 4 cut(s) 72, 117, 165, 285
BstUI CGCG 4 cut(s) 65, 105, 163, 212
BstV1I GCAGC 3 cut(s) 30, 36, 600
BsuRI GGCC 2 cut(s) 72, 170
BtgI CCRYGG 1 cut(s) 103
BtrI CACGTC 1 cut(s) 10
BtsCI GGATG 3 cut(s) 399, 424, 460
Cac8I GCNNGC 3 cut(s) 163, 300, 304
CfoI GCGC 6 cut(s) 67, 94, 163, 165, 212, 308
Cfr13I GGNCC 3 cut(s) 108, 168, 283
Cfr42I CCGCGG 1 cut(s) 106
CseI GACGC 3 cut(s) 65, 226, 238
DdeI CTNAG 2 cut(s) 462, 522
DpnI GATC 1 cut(s) 510
DpnII GATC 1 cut(s) 508
EaeI YGGCCR 1 cut(s) 70
Eam1104I CTCTTC 1 cut(s) 134
EarI CTCTTC 1 cut(s) 134
EciI GGCGGA 1 cut(s) 350
Ecl136II GAGCTC 1 cut(s) 154
Eco24I GRGCYC 2 cut(s) 156, 203
Eco47I GGWCC 2 cut(s) 108, 283
Eco53kI GAGCTC 1 cut(s) 154
Eco57I CTGAAG 1 cut(s) 242
Eco91I GGTNACC 1 cut(s) 76
EcoICRI GAGCTC 1 cut(s) 154
EcoNI CCTNNNNNAGG 1 cut(s) 26
EcoO109I RGGNCCY 1 cut(s) 108
EcoO65I GGTNACC 1 cut(s) 76
EcoRII CCWGG 2 cut(s) 72, 117
EcoT38I GRGCYC 2 cut(s) 156, 203
Esp3I CGTCTC 1 cut(s) 16
FaiI YATR 3 cut(s) 426, 468, 486
FaqI GGGAC 2 cut(s) 121, 490
FauI CCCGC 2 cut(s) 98, 291
FblI GTMKAC 1 cut(s) 243
Fnu4HI GCNGC 8 cut(s) 44, 47, 50, 60, 63, 309, 333, 614
FokI GGATG 3 cut(s) 406, 431, 467
FriOI GRGCYC 2 cut(s) 156, 203
Fsp4HI GCNGC 8 cut(s) 44, 47, 50, 60, 63, 309, 333, 614
FspBI CTAG 1 cut(s) 342
GlaI GCGC 6 cut(s) 66, 93, 162, 164, 211, 307
GluI GCNGC 8 cut(s) 44, 47, 50, 60, 63, 309, 333, 614
GsaI CCCAGC 1 cut(s) 609
HaeII RGCGCY 1 cut(s) 95
HaeIII GGCC 2 cut(s) 72, 170
HapII CCGG 7 cut(s) 53, 166, 178, 184, 196, 287, 317
HgaI GACGC 3 cut(s) 65, 226, 238
HhaI GCGC 6 cut(s) 67, 94, 163, 165, 212, 308
Hin1I GRCGYC 2 cut(s) 57, 249
Hin6I GCGC 6 cut(s) 65, 92, 161, 163, 210, 306
HinP1I GCGC 6 cut(s) 65, 92, 161, 163, 210, 306
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HindIII AAGCTT 1 cut(s) 530
HinfI GANTC 2 cut(s) 124, 338
HpaII CCGG 7 cut(s) 53, 166, 178, 184, 196, 287, 317
HphI GGTGA 4 cut(s) 70, 226, 370, 571
Hpy166II GTNNAC 1 cut(s) 244
Hpy188I TCNGA 2 cut(s) 151, 193
Hpy188III TCNNGA 1 cut(s) 512
Hpy8I GTNNAC 1 cut(s) 244
Hpy99I CGWCG 3 cut(s) 140, 245, 248
HpyAV CCTTC 4 cut(s) 217, 223, 348, 541
HpyCH4IV ACGT 1 cut(s) 9
HpyCH4V TGCA 1 cut(s) 593
HpyF10VI GCNNNNNNNGC 6 cut(s) 49, 160, 167, 207, 308, 322
HpyF3I CTNAG 2 cut(s) 462, 522
HpySE526I ACGT 1 cut(s) 9
Hsp92I GRCGYC 2 cut(s) 57, 249
HspAI GCGC 6 cut(s) 65, 92, 161, 163, 210, 306
KflI GGGWCCC 1 cut(s) 108
KspI CCGCGG 1 cut(s) 106
Kzo9I GATC 1 cut(s) 508
LmnI GCTCC 5 cut(s) 146, 180, 186, 198, 518
Lsp1109I GCAGC 3 cut(s) 30, 36, 600
MaeI CTAG 1 cut(s) 342
MaeII ACGT 1 cut(s) 9
MaeIII GTNAC 2 cut(s) 76, 214
MalI GATC 1 cut(s) 510
MboI GATC 1 cut(s) 508
MboII GAAGA 2 cut(s) 121, 124
MhlI GDGCHC 4 cut(s) 156, 177, 203, 240
MlyI GAGTC 1 cut(s) 347
MmeI TCCRAC 2 cut(s) 216, 426
MroXI GAANNNNTTC 1 cut(s) 128
MspA1I CMGCKG 1 cut(s) 105
MspI CCGG 7 cut(s) 53, 166, 178, 184, 196, 287, 317
MspR9I CCNGG 4 cut(s) 74, 119, 167, 287
MvaI CCWGG 2 cut(s) 74, 119
MvnI CGCG 4 cut(s) 65, 105, 163, 212
MwoI GCNNNNNNNGC 6 cut(s) 49, 160, 167, 207, 308, 322
NciI CCSGG 2 cut(s) 167, 287
NdeII GATC 1 cut(s) 508
NlaIV GGNNCC 6 cut(s) 109, 110, 142, 182, 200, 285
NmeAIII GCCGAG 3 cut(s) 137, 281, 337
NmuCI GTSAC 2 cut(s) 76, 214
PalAI GGCGCGCC 1 cut(s) 161
PauI GCGCGC 1 cut(s) 161
PcsI WCGNNNNNNNCGW 2 cut(s) 224, 246
PdmI GAANNNNTTC 1 cut(s) 128
PfeI GAWTC 1 cut(s) 124
PflFI GACNNNGTC 1 cut(s) 248
PkrI GCNGC 8 cut(s) 45, 48, 51, 61, 64, 310, 334, 615
PleI GAGTC 1 cut(s) 346
PpsI GAGTC 1 cut(s) 346
PpuMI RGGWCCY 1 cut(s) 108
Psp124BI GAGCTC 1 cut(s) 156
Psp5II RGGWCCY 1 cut(s) 108
Psp6I CCWGG 2 cut(s) 72, 117
PspEI GGTNACC 1 cut(s) 76
PspFI CCCAGC 1 cut(s) 605
PspGI CCWGG 2 cut(s) 72, 117
PspN4I GGNNCC 6 cut(s) 109, 110, 142, 182, 200, 285
PspPI GGNCC 3 cut(s) 108, 168, 283
PspPPI RGGWCCY 1 cut(s) 108
PsyI GACNNNGTC 1 cut(s) 248
PteI GCGCGC 1 cut(s) 161
SacI GAGCTC 1 cut(s) 156
SacII CCGCGG 1 cut(s) 106
SalI GTCGAC 1 cut(s) 242
SatI GCNGC 8 cut(s) 44, 47, 50, 60, 63, 309, 333, 614
Sau3AI GATC 1 cut(s) 508
Sau96I GGNCC 3 cut(s) 108, 168, 283
SchI GAGTC 1 cut(s) 347
ScrFI CCNGG 4 cut(s) 74, 119, 167, 287
SduI GDGCHC 4 cut(s) 156, 177, 203, 240
Sfr303I CCGCGG 1 cut(s) 106
SgrBI CCGCGG 1 cut(s) 106
SgrDI CGTCGACG 1 cut(s) 242
SgsI GGCGCGCC 1 cut(s) 161
SinI GGWCC 2 cut(s) 108, 283
SmlI CTYRAG 1 cut(s) 533
SmoI CTYRAG 1 cut(s) 533
SspMI CTAG 1 cut(s) 342
SstI GAGCTC 1 cut(s) 156
StyD4I CCNGG 4 cut(s) 72, 117, 165, 285
TaiI ACGT 1 cut(s) 12
TaqI TCGA 4 cut(s) 33, 135, 243, 601
TauI GCSGC 5 cut(s) 49, 62, 65, 311, 335
TfiI GAWTC 1 cut(s) 124
TseFI GTSAC 2 cut(s) 76, 214
TseI GCWGC 3 cut(s) 43, 49, 613
Tsp45I GTSAC 2 cut(s) 76, 214
Tth111I GACNNNGTC 1 cut(s) 248
VpaK11BI GGWCC 2 cut(s) 108, 283
XagI CCTNNNNNAGG 1 cut(s) 26
XcmI CCANNNNNNNNNTGG 1 cut(s) 22
XmiI GTMKAC 1 cut(s) 243
XmnI GAANNNNTTC 1 cut(s) 128
XspI CTAG 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.