MD17G1216500.v1.1

Triphosphate tunel metalloenzyme 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002663
Physical Location & Seq
Reverse (-)
26328328 .. 26329050
723 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1216500.v1.1.491

Sequence Viewer

Length: 723 bp
ATGGGGACACCAGATCCGCAGCCACATTTCCCACCACCAAGTGCTTCCATATCAAGTTATCAACGACATTGTTCACTCGTTTGGTCCTTCCTTCAGCAAACCCCCAAAGCAATGGAAGTGGAAGTGAAGCTCCGCCTCCCAGACGCCGCCGCACACCAAAAGCTCACCGACCTCCTCTCCCCCTTCCACACCCAAACCCTAATCCAAGAGAACATCTTCTTCGACGGCTCCAACTCCGAGCTCTCCTCTAACCTCGCCATCCTCCGCCTCCGTTTCTACGACGTCGACAGGCGCTGCGTCCTCTGCCTCAAGGCCAAACCCGTCATCTCCGACGGCGTCAGCCGAGTCCTAGAGCTGGAAGAGCCCTTCGACCCTGTCCTCGGCCGCGTCTGCGTGGCCGAGCCGTGGAGGCTTACGGTCGTTGACTCCTCCGACATCTTAAAGCGCGTGAGGGAGGAATACAGTGTCGGAGGAGGGCAAGGGTTTGTGTGCTTGGGTGGGTTTAGGAATGTGAGGGGTGTGTATAAGTGGAAGGGGCTGAAGATAGAGGTGGACGAGAGTATTTACGATTTTGGGACGTGTTATGAGCTCGAGTGCGAGAGCTCCAACCCTGACAGGGATAAGCAGGTTCTGGAAAACTTGTTGGAAGAGAACGGGATTAGGTTTCGGTACTCGGAGGTCTCCAAGTTTGCGATTTTCCGGTCCGGGAAGTTGCCGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CYTH

Protein Analysis

241

Amino Acids

27.23

Weight (kDa)

5.54

Isoelectric Point (pI)

50.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CYTH PF01928 38 - 229 5.2e-19 CYTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 285
Acc36I ACCTGC 1 cut(s) 616
AccI GTMKAC 1 cut(s) 285
AccII CGCG 2 cut(s) 387, 447
AciI CCGC 6 cut(s) 17, 133, 147, 150, 265, 385
AclWI GGATC 1 cut(s) 8
AcoI YGGCCR 2 cut(s) 382, 396
AcuI CTGAAG 2 cut(s) 77, 560
AcyI GRCGYC 3 cut(s) 144, 282, 336
AdeI CACNNNGTG 1 cut(s) 41
AfaI GTAC 1 cut(s) 671
AfiI CCNNNNNNNGG 4 cut(s) 355, 380, 405, 616
AflIII ACRYGT 1 cut(s) 578
AjiI CACGTC 1 cut(s) 579
AluBI AGCT 6 cut(s) 130, 163, 241, 355, 589, 603
AluI AGCT 6 cut(s) 130, 163, 241, 355, 589, 603
Alw21I GWGCWC 3 cut(s) 243, 591, 605
Alw26I GTCTC 1 cut(s) 685
AlwI GGATC 1 cut(s) 8
AlwNI CAGNNNCTG 2 cut(s) 294, 631
Ama87I CYCGRG 1 cut(s) 590
AoxI GGCC 3 cut(s) 312, 382, 396
ApeKI GCWGC 2 cut(s) 19, 294
Asp700I GAANNNNTTC 1 cut(s) 215
AspLEI GCGC 2 cut(s) 294, 447
AspS9I GGNCC 2 cut(s) 84, 702
AsuC2I CCSGG 1 cut(s) 706
AsuHPI GGTGA 1 cut(s) 157
AvaI CYCGRG 1 cut(s) 590
AvaII GGWCC 2 cut(s) 84, 702
BanII GRGCYC 4 cut(s) 243, 366, 591, 605
Bbv12I GWGCWC 3 cut(s) 243, 591, 605
BbvI GCAGC 2 cut(s) 31, 281
BccI CCATC 1 cut(s) 266
BceAI ACGGC 3 cut(s) 241, 349, 388
BcnI CCSGG 1 cut(s) 706
BcoDI GTCTC 1 cut(s) 685
BfaI CTAG 1 cut(s) 350
BfoI RGCGCY 1 cut(s) 295
BfuAI ACCTGC 1 cut(s) 616
BglI GCCNNNNNGGC 1 cut(s) 409
BisI GCNGC 5 cut(s) 20, 147, 150, 295, 385
BlsI GCNGC 5 cut(s) 21, 148, 151, 296, 386
Bme1390I CCNGG 1 cut(s) 706
Bme18I GGWCC 2 cut(s) 84, 702
BmeT110I CYCGRG 1 cut(s) 590
BmgBI CACGTC 1 cut(s) 579
BmgT120I GGNCC 2 cut(s) 84, 702
BmiI GGNNCC 1 cut(s) 229
BmrFI CCNGG 1 cut(s) 706
BplI GAGNNNNNCTC 2 cut(s) 230, 262
BpuEI CTTGAG 1 cut(s) 293
BpuMI CCSGG 1 cut(s) 706
BsaHI GRCGYC 3 cut(s) 144, 282, 336
BsaI GGTCTC 1 cut(s) 685
BsaJI CCNNGG 2 cut(s) 379, 404
BsaWI WCCGGW 1 cut(s) 699
BsaXI ACNNNNNCTCC 2 cut(s) 161, 191
Bsc4I CCNNNNNNNGG 4 cut(s) 355, 380, 405, 616
Bse3DI GCAATG 1 cut(s) 117
BseDI CCNNGG 2 cut(s) 379, 404
BseGI GGATG 1 cut(s) 258
BseLI CCNNNNNNNGG 4 cut(s) 355, 380, 405, 616
BseMI GCAATG 1 cut(s) 117
BseRI GAGGAG 4 cut(s) 164, 235, 418, 486
BseX3I CGGCCG 1 cut(s) 382
BseXI GCAGC 2 cut(s) 31, 281
Bsh1236I CGCG 2 cut(s) 387, 447
Bsh1285I CGRYCG 2 cut(s) 385, 420
BshFI GGCC 3 cut(s) 314, 384, 398
BsiEI CGRYCG 2 cut(s) 385, 420
BsiHKAI GWGCWC 3 cut(s) 243, 591, 605
BsiHKCI CYCGRG 1 cut(s) 590
BsiSI CCGG 3 cut(s) 700, 705, 716
BslFI GGGAC 2 cut(s) 19, 589
BslI CCNNNNNNNGG 4 cut(s) 355, 380, 405, 616
BsmAI GTCTC 1 cut(s) 685
BsmFI GGGAC 2 cut(s) 19, 589
BsnI GGCC 3 cut(s) 314, 384, 398
Bso31I GGTCTC 1 cut(s) 685
BsoBI CYCGRG 1 cut(s) 590
Bsp1286I GDGCHC 4 cut(s) 243, 366, 591, 605
Bsp143I GATC 1 cut(s) 13
BspACI CCGC 6 cut(s) 17, 133, 147, 150, 265, 385
BspANI GGCC 3 cut(s) 314, 384, 398
BspFNI CGCG 2 cut(s) 387, 447
BspLI GGNNCC 1 cut(s) 229
BspMI ACCTGC 1 cut(s) 616
BspPI GGATC 1 cut(s) 8
BspQI GCTCTTC 1 cut(s) 354
BspTNI GGTCTC 1 cut(s) 685
BsrDI GCAATG 1 cut(s) 117
BssECI CCNNGG 2 cut(s) 379, 404
BssMI GATC 1 cut(s) 13
BssNI GRCGYC 3 cut(s) 144, 282, 336
Bst4CI ACNGT 2 cut(s) 418, 464
Bst6I CTCTTC 2 cut(s) 354, 642
BstACI GRCGYC 3 cut(s) 144, 282, 336
BstDSI CCRYGG 1 cut(s) 404
BstF5I GGATG 1 cut(s) 258
BstFNI CGCG 2 cut(s) 387, 447
BstH2I RGCGCY 1 cut(s) 295
BstHHI GCGC 2 cut(s) 294, 447
BstKTI GATC 1 cut(s) 16
BstMAI GTCTC 1 cut(s) 685
BstMBI GATC 1 cut(s) 13
BstMCI CGRYCG 2 cut(s) 385, 420
BstMWI GCNNNNNNNGC 4 cut(s) 303, 361, 390, 409
BstSCI CCNGG 1 cut(s) 704
BstUI CGCG 2 cut(s) 387, 447
BstV1I GCAGC 2 cut(s) 31, 281
BstX2I RGATCY 1 cut(s) 13
BstXI CCANNNNNNTGG 1 cut(s) 112
BstYI RGATCY 1 cut(s) 13
BstZI CGGCCG 1 cut(s) 382
BsuRI GGCC 3 cut(s) 314, 384, 398
BtgI CCRYGG 1 cut(s) 404
BtrI CACGTC 1 cut(s) 579
BtsCI GGATG 1 cut(s) 258
BtsIMutI CAGTG 1 cut(s) 469
BveI ACCTGC 1 cut(s) 616
CaiI CAGNNNCTG 2 cut(s) 294, 631
CfoI GCGC 2 cut(s) 294, 447
Cfr13I GGNCC 2 cut(s) 84, 702
CpoI CGGWCCG 1 cut(s) 702
CseI GACGC 4 cut(s) 152, 286, 325, 376
Csp6I GTAC 1 cut(s) 670
CspCI CAANNNNNGTGG 2 cut(s) 99, 134
CspI CGGWCCG 1 cut(s) 702
CviQI GTAC 1 cut(s) 670
DpnI GATC 1 cut(s) 15
DpnII GATC 1 cut(s) 13
DraIII CACNNNGTG 1 cut(s) 41
EaeI YGGCCR 2 cut(s) 382, 396
EagI CGGCCG 1 cut(s) 382
Eam1104I CTCTTC 2 cut(s) 354, 642
EarI CTCTTC 2 cut(s) 354, 642
EciI GGCGGA 2 cut(s) 122, 254
Ecl136II GAGCTC 3 cut(s) 241, 589, 603
EclXI CGGCCG 1 cut(s) 382
Eco24I GRGCYC 4 cut(s) 243, 366, 591, 605
Eco31I GGTCTC 1 cut(s) 685
Eco47I GGWCC 2 cut(s) 84, 702
Eco52I CGGCCG 1 cut(s) 382
Eco53kI GAGCTC 3 cut(s) 241, 589, 603
Eco57I CTGAAG 2 cut(s) 77, 560
Eco88I CYCGRG 1 cut(s) 590
EcoICRI GAGCTC 3 cut(s) 241, 589, 603
EcoT38I GRGCYC 4 cut(s) 243, 366, 591, 605
FaiI YATR 3 cut(s) 50, 525, 585
FaqI GGGAC 2 cut(s) 19, 589
FblI GTMKAC 1 cut(s) 285
Fnu4HI GCNGC 5 cut(s) 20, 147, 150, 295, 385
FokI GGATG 1 cut(s) 245
FriOI GRGCYC 4 cut(s) 243, 366, 591, 605
Fsp4HI GCNGC 5 cut(s) 20, 147, 150, 295, 385
FspBI CTAG 1 cut(s) 350
GlaI GCGC 2 cut(s) 293, 446
GluI GCNGC 5 cut(s) 20, 147, 150, 295, 385
HaeII RGCGCY 1 cut(s) 295
HaeIII GGCC 3 cut(s) 314, 384, 398
HapII CCGG 3 cut(s) 700, 705, 716
HgaI GACGC 4 cut(s) 152, 286, 325, 376
HhaI GCGC 2 cut(s) 294, 447
Hin1I GRCGYC 3 cut(s) 144, 282, 336
Hin6I GCGC 2 cut(s) 292, 445
HinP1I GCGC 2 cut(s) 292, 445
HincII GTYRAC 2 cut(s) 286, 424
HindII GTYRAC 2 cut(s) 286, 424
HinfI GANTC 2 cut(s) 345, 425
HpaII CCGG 3 cut(s) 700, 705, 716
HphI GGTGA 1 cut(s) 157
Hpy166II GTNNAC 4 cut(s) 74, 286, 424, 553
Hpy188I TCNGA 5 cut(s) 238, 331, 433, 470, 676
Hpy188III TCNNGA 1 cut(s) 632
Hpy8I GTNNAC 4 cut(s) 74, 286, 424, 553
Hpy99I CGWCG 4 cut(s) 227, 284, 287, 335
HpyAV CCTTC 5 cut(s) 97, 101, 193, 376, 526
HpyCH4III ACNGT 2 cut(s) 418, 464
HpyCH4IV ACGT 2 cut(s) 282, 578
HpyF10VI GCNNNNNNNGC 4 cut(s) 303, 361, 390, 409
HpySE526I ACGT 2 cut(s) 282, 578
Hsp92I GRCGYC 3 cut(s) 144, 282, 336
HspAI GCGC 2 cut(s) 292, 445
Kzo9I GATC 1 cut(s) 13
LguI GCTCTTC 1 cut(s) 354
LmnI GCTCC 3 cut(s) 135, 233, 608
Lsp1109I GCAGC 2 cut(s) 31, 281
MaeI CTAG 1 cut(s) 350
MaeII ACGT 2 cut(s) 282, 578
MalI GATC 1 cut(s) 15
MboI GATC 1 cut(s) 13
MboII GAAGA 5 cut(s) 208, 211, 371, 553, 659
MflI RGATCY 1 cut(s) 13
MhlI GDGCHC 4 cut(s) 243, 366, 591, 605
MlyI GAGTC 2 cut(s) 354, 419
MmeI TCCRAC 6 cut(s) 255, 354, 448, 456, 624, 630
MroXI GAANNNNTTC 1 cut(s) 215
MseI TTAA 1 cut(s) 440
MspI CCGG 3 cut(s) 700, 705, 716
MspR9I CCNGG 1 cut(s) 706
MvnI CGCG 2 cut(s) 387, 447
MwoI GCNNNNNNNGC 4 cut(s) 303, 361, 390, 409
NciI CCSGG 1 cut(s) 706
NdeII GATC 1 cut(s) 13
NlaIV GGNNCC 1 cut(s) 229
NmeAIII GCCGAG 3 cut(s) 360, 368, 424
PaeR7I CTCGAG 1 cut(s) 590
PciSI GCTCTTC 1 cut(s) 354
PdmI GAANNNNTTC 1 cut(s) 215
PflFI GACNNNGTC 2 cut(s) 335, 374
PfoI TCCNGGA 1 cut(s) 704
PkrI GCNGC 5 cut(s) 21, 148, 151, 296, 386
PleI GAGTC 2 cut(s) 353, 419
PpsI GAGTC 2 cut(s) 353, 419
Psp124BI GAGCTC 3 cut(s) 243, 591, 605
PspN4I GGNNCC 1 cut(s) 229
PspPI GGNCC 2 cut(s) 84, 702
PspXI VCTCGAGB 1 cut(s) 590
PstNI CAGNNNCTG 2 cut(s) 294, 631
PsuI RGATCY 1 cut(s) 13
PsyI GACNNNGTC 2 cut(s) 335, 374
RsaI GTAC 1 cut(s) 671
RsaNI GTAC 1 cut(s) 670
Rsr2I CGGWCCG 1 cut(s) 702
RsrII CGGWCCG 1 cut(s) 702
SacI GAGCTC 3 cut(s) 243, 591, 605
SalI GTCGAC 1 cut(s) 284
SapI GCTCTTC 1 cut(s) 354
SaqAI TTAA 1 cut(s) 440
SatI GCNGC 5 cut(s) 20, 147, 150, 295, 385
Sau3AI GATC 1 cut(s) 13
Sau96I GGNCC 2 cut(s) 84, 702
SchI GAGTC 2 cut(s) 354, 419
ScrFI CCNGG 1 cut(s) 706
SduI GDGCHC 4 cut(s) 243, 366, 591, 605
Sfr274I CTCGAG 1 cut(s) 590
SinI GGWCC 2 cut(s) 84, 702
SlaI CTCGAG 1 cut(s) 590
SmlI CTYRAG 2 cut(s) 308, 590
SmoI CTYRAG 2 cut(s) 308, 590
SsiI CCGC 6 cut(s) 17, 133, 147, 150, 265, 385
SspMI CTAG 1 cut(s) 350
SstI GAGCTC 3 cut(s) 243, 591, 605
StyD4I CCNGG 1 cut(s) 704
TaaI ACNGT 2 cut(s) 418, 464
TaiI ACGT 2 cut(s) 285, 581
TaqI TCGA 4 cut(s) 222, 285, 369, 591
TauI GCSGC 3 cut(s) 149, 152, 387
Tru1I TTAA 1 cut(s) 440
Tru9I TTAA 1 cut(s) 440
TscAI CASTG 1 cut(s) 469
TseI GCWGC 2 cut(s) 19, 294
TspGWI ACGGA 1 cut(s) 260
TspRI CASTG 1 cut(s) 469
Tth111I GACNNNGTC 2 cut(s) 335, 374
VpaK11BI GGWCC 2 cut(s) 84, 702
XhoI CTCGAG 1 cut(s) 590
XmiI GTMKAC 1 cut(s) 285
XmnI GAANNNNTTC 1 cut(s) 215
XspI CTAG 1 cut(s) 350
ZraI GACGTC 1 cut(s) 283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.