Rh2BG025600

Triphosphate tunel metalloenzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0002663
Physical Location & Seq
Reverse (-)
1711626 .. 1713631
2006 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG025600.1

Sequence Viewer

Length: 612 bp
ATGTCCAAAGCCCTAATGGAGGTCGAGGTCAAGCTGAGGCTGCCGGACGCCGCCGCCCACGGCCAGGTCACCGCTCTTCTAGCGCCGTTCCACCTCGGCACCCACCGCCAGGAGAATCTCTTCTTCGACGGCTCCAAGTCCGAGCTCTCGGCGCGCCGGGCCGCGCTCCGGCTACGCTTCTCCGACCGGAGCCCCCTCTGCGCCGTGACGCTGAAGGCGAAGGCGGTGCTGGTCGACGGCGTGAGCCGGGTGGAGGAGGACGAGGAGGAGATGGACCCGGCGGTGGGGCGGGCGTGCGCCGCCATGCCGGAGAAGATGATGGCGGCGGAGTCTAGGGTTTTGAGGAGGGTGAGGGAGGAGTTTGGGGTTTTAGGGTTTGTGGGGATGGGAGGGTTTGGGAATGTGAGGGATGTGTATGATTGGAAAGGGTTGAAATTGGAGGTGGATGAGACTAAGTATGAGTTTGGGACTTGTTATGAGATTGAGTGTGAGAGCGCTGATCCTGATGGGGCTAAGGAGAAGCTTGAGGAGTTCTTGAAGGAAAATGGGGTGAGGTATTCGTATTCTCAAGTGTCCAAGTTTGCGACTTTTCGAGCTGGGAAGCTGCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CYTH

Protein Analysis

203

Amino Acids

22.61

Weight (kDa)

5.58

Isoelectric Point (pI)

48.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CYTH PF01928 6 - 183 5.3e-20 CYTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 98
AccBSI CCGCTC 1 cut(s) 74
AccI GTMKAC 1 cut(s) 234
AccII CGCG 2 cut(s) 154, 164
AclWI GGATC 1 cut(s) 494
AcoI YGGCCR 1 cut(s) 61
AcuI CTGAAG 1 cut(s) 233
AcyI GRCGYC 1 cut(s) 48
AfeI AGCGCT 1 cut(s) 496
AfiI CCNNNNNNNGG 7 cut(s) 19, 64, 109, 168, 253, 283, 284
AgsI TTSAA 2 cut(s) 433, 538
AjnI CCWGG 2 cut(s) 63, 108
AluBI AGCT 5 cut(s) 34, 145, 523, 596, 604
AluI AGCT 5 cut(s) 34, 145, 523, 596, 604
Alw21I GWGCWC 1 cut(s) 147
Alw26I GTCTC 1 cut(s) 443
AlwI GGATC 1 cut(s) 494
Aor51HI AGCGCT 1 cut(s) 496
AoxI GGCC 2 cut(s) 61, 159
ApeKI GCWGC 2 cut(s) 40, 604
AscI GGCGCGCC 1 cut(s) 152
Asp700I GAANNNNTTC 1 cut(s) 119
AspLEI GCGC 7 cut(s) 85, 154, 156, 166, 203, 299, 497
AspS9I GGNCC 2 cut(s) 159, 274
AsuC2I CCSGG 3 cut(s) 158, 248, 278
AsuHPI GGTGA 3 cut(s) 61, 361, 562
AvaII GGWCC 1 cut(s) 274
BanI GGYRCC 1 cut(s) 98
BanII GRGCYC 2 cut(s) 147, 194
Bbv12I GWGCWC 1 cut(s) 147
BbvCI CCTCAGC 1 cut(s) 35
BbvI GCAGC 2 cut(s) 27, 591
BccI CCATC 4 cut(s) 265, 313, 379, 500
BceAI ACGGC 5 cut(s) 70, 76, 145, 188, 253
BcgI CGANNNNNNTGC 2 cut(s) 208, 242
BciT130I CCWGG 2 cut(s) 65, 110
BcnI CCSGG 3 cut(s) 158, 248, 278
BcoDI GTCTC 1 cut(s) 443
BfaI CTAG 2 cut(s) 80, 333
BfoI RGCGCY 2 cut(s) 86, 498
BisI GCNGC 7 cut(s) 41, 51, 54, 162, 300, 324, 605
BlsI GCNGC 7 cut(s) 42, 52, 55, 163, 301, 325, 606
Bme1390I CCNGG 5 cut(s) 65, 110, 158, 248, 278
Bme18I GGWCC 1 cut(s) 274
BmgT120I GGNCC 2 cut(s) 159, 274
BmiI GGNNCC 4 cut(s) 100, 133, 191, 276
BmrFI CCNGG 5 cut(s) 65, 110, 158, 248, 278
Bpu10I CCTNAGC 2 cut(s) 35, 513
BpuEI CTTGAG 2 cut(s) 545, 552
BpuMI CCSGG 3 cut(s) 158, 248, 278
BsaHI GRCGYC 1 cut(s) 48
BsaJI CCNNGG 2 cut(s) 58, 94
BsaWI WCCGGW 1 cut(s) 186
Bsc4I CCNNNNNNNGG 7 cut(s) 19, 64, 109, 168, 253, 283, 284
BseBI CCWGG 2 cut(s) 65, 110
BseDI CCNNGG 2 cut(s) 58, 94
BseGI GGATG 3 cut(s) 390, 415, 451
BseLI CCNNNNNNNGG 7 cut(s) 19, 64, 109, 168, 253, 283, 284
BseMII CTCAG 1 cut(s) 26
BsePI GCGCGC 1 cut(s) 152
BseRI GAGGAG 6 cut(s) 269, 278, 281, 358, 371, 542
BseXI GCAGC 2 cut(s) 27, 591
BseYI CCCAGC 1 cut(s) 596
Bsh1236I CGCG 2 cut(s) 154, 164
Bsh1285I CGRYCG 1 cut(s) 187
BshFI GGCC 2 cut(s) 63, 161
BshNI GGYRCC 1 cut(s) 98
BsiEI CGRYCG 1 cut(s) 187
BsiHKAI GWGCWC 1 cut(s) 147
BsiSI CCGG 7 cut(s) 44, 157, 169, 187, 247, 278, 308
BslFI GGGAC 1 cut(s) 481
BslI CCNNNNNNNGG 7 cut(s) 19, 64, 109, 168, 253, 283, 284
BsmAI GTCTC 1 cut(s) 443
BsmFI GGGAC 1 cut(s) 481
BsnI GGCC 2 cut(s) 63, 161
Bsp1286I GDGCHC 2 cut(s) 147, 194
Bsp143I GATC 1 cut(s) 499
BspANI GGCC 2 cut(s) 63, 161
BspCNI CTCAG 1 cut(s) 27
BspFNI CGCG 2 cut(s) 154, 164
BspLI GGNNCC 4 cut(s) 100, 133, 191, 276
BspPI GGATC 1 cut(s) 494
BspQI GCTCTTC 1 cut(s) 81
BspT107I GGYRCC 1 cut(s) 98
BsrBI CCGCTC 1 cut(s) 74
BssECI CCNNGG 2 cut(s) 58, 94
BssHII GCGCGC 1 cut(s) 152
BssMI GATC 1 cut(s) 499
BssNI GRCGYC 1 cut(s) 48
Bst2UI CCWGG 2 cut(s) 65, 110
Bst6I CTCTTC 2 cut(s) 81, 125
BstACI GRCGYC 1 cut(s) 48
BstC8I GCNNGC 3 cut(s) 154, 291, 295
BstDEI CTNAG 4 cut(s) 35, 453, 513, 609
BstDSI CCRYGG 1 cut(s) 58
BstEII GGTNACC 1 cut(s) 67
BstENI CCTNNNNNAGG 1 cut(s) 17
BstF5I GGATG 3 cut(s) 390, 415, 451
BstFNI CGCG 2 cut(s) 154, 164
BstH2I RGCGCY 2 cut(s) 86, 498
BstHHI GCGC 7 cut(s) 85, 154, 156, 166, 203, 299, 497
BstKTI GATC 1 cut(s) 502
BstMAI GTCTC 1 cut(s) 443
BstMBI GATC 1 cut(s) 499
BstMCI CGRYCG 1 cut(s) 187
BstMWI GCNNNNNNNGC 7 cut(s) 40, 80, 105, 151, 158, 198, 299
BstNI CCWGG 2 cut(s) 65, 110
BstPI GGTNACC 1 cut(s) 67
BstSCI CCNGG 5 cut(s) 63, 108, 156, 246, 276
BstUI CGCG 2 cut(s) 154, 164
BstV1I GCAGC 2 cut(s) 27, 591
BsuRI GGCC 2 cut(s) 63, 161
BtgI CCRYGG 1 cut(s) 58
BtsCI GGATG 3 cut(s) 390, 415, 451
Cac8I GCNNGC 3 cut(s) 154, 291, 295
CfoI GCGC 7 cut(s) 85, 154, 156, 166, 203, 299, 497
Cfr13I GGNCC 2 cut(s) 159, 274
CseI GACGC 2 cut(s) 56, 217
CviAII CATG 1 cut(s) 304
DdeI CTNAG 4 cut(s) 35, 453, 513, 609
DpnI GATC 1 cut(s) 501
DpnII GATC 1 cut(s) 499
EaeI YGGCCR 1 cut(s) 61
Eam1104I CTCTTC 2 cut(s) 81, 125
EarI CTCTTC 2 cut(s) 81, 125
EciI GGCGGA 1 cut(s) 341
Ecl136II GAGCTC 1 cut(s) 145
Eco24I GRGCYC 2 cut(s) 147, 194
Eco47I GGWCC 1 cut(s) 274
Eco47III AGCGCT 1 cut(s) 496
Eco53kI GAGCTC 1 cut(s) 145
Eco57I CTGAAG 1 cut(s) 233
Eco91I GGTNACC 1 cut(s) 67
EcoICRI GAGCTC 1 cut(s) 145
EcoNI CCTNNNNNAGG 1 cut(s) 17
EcoO65I GGTNACC 1 cut(s) 67
EcoRII CCWGG 2 cut(s) 63, 108
EcoT38I GRGCYC 2 cut(s) 147, 194
FaeI CATG 1 cut(s) 307
FaiI YATR 4 cut(s) 305, 417, 459, 477
FaqI GGGAC 1 cut(s) 481
FatI CATG 1 cut(s) 303
FauI CCCGC 1 cut(s) 282
FblI GTMKAC 1 cut(s) 234
Fnu4HI GCNGC 7 cut(s) 41, 51, 54, 162, 300, 324, 605
FokI GGATG 3 cut(s) 397, 422, 458
FriOI GRGCYC 2 cut(s) 147, 194
Fsp4HI GCNGC 7 cut(s) 41, 51, 54, 162, 300, 324, 605
FspBI CTAG 2 cut(s) 80, 333
GlaI GCGC 7 cut(s) 84, 153, 155, 165, 202, 298, 496
GluI GCNGC 7 cut(s) 41, 51, 54, 162, 300, 324, 605
GsaI CCCAGC 1 cut(s) 600
HaeII RGCGCY 2 cut(s) 86, 498
HaeIII GGCC 2 cut(s) 63, 161
HapII CCGG 7 cut(s) 44, 157, 169, 187, 247, 278, 308
HgaI GACGC 2 cut(s) 56, 217
HhaI GCGC 7 cut(s) 85, 154, 156, 166, 203, 299, 497
Hin1I GRCGYC 1 cut(s) 48
Hin1II CATG 1 cut(s) 307
Hin6I GCGC 7 cut(s) 83, 152, 154, 164, 201, 297, 495
HinP1I GCGC 7 cut(s) 83, 152, 154, 164, 201, 297, 495
HincII GTYRAC 1 cut(s) 235
HindII GTYRAC 1 cut(s) 235
HindIII AAGCTT 1 cut(s) 521
HinfI GANTC 2 cut(s) 115, 329
HpaII CCGG 7 cut(s) 44, 157, 169, 187, 247, 278, 308
HphI GGTGA 3 cut(s) 61, 361, 562
Hpy166II GTNNAC 1 cut(s) 235
Hpy188I TCNGA 2 cut(s) 142, 184
Hpy188III TCNNGA 2 cut(s) 503, 535
Hpy8I GTNNAC 1 cut(s) 235
Hpy99I CGWCG 2 cut(s) 131, 239
HpyAV CCTTC 3 cut(s) 208, 214, 532
HpyF10VI GCNNNNNNNGC 7 cut(s) 40, 80, 105, 151, 158, 198, 299
HpyF3I CTNAG 4 cut(s) 35, 453, 513, 609
Hsp92I GRCGYC 1 cut(s) 48
Hsp92II CATG 1 cut(s) 307
HspAI GCGC 7 cut(s) 83, 152, 154, 164, 201, 297, 495
Kzo9I GATC 1 cut(s) 499
LguI GCTCTTC 1 cut(s) 81
LmnI GCTCC 3 cut(s) 137, 171, 189
Lsp1109I GCAGC 2 cut(s) 27, 591
MaeI CTAG 2 cut(s) 80, 333
MaeIII GTNAC 2 cut(s) 67, 205
MalI GATC 1 cut(s) 501
MbiI CCGCTC 1 cut(s) 74
MboI GATC 1 cut(s) 499
MboII GAAGA 4 cut(s) 68, 112, 115, 325
MhlI GDGCHC 2 cut(s) 147, 194
MluCI AATT 1 cut(s) 434
MlyI GAGTC 1 cut(s) 338
MmeI TCCRAC 1 cut(s) 207
MroXI GAANNNNTTC 1 cut(s) 119
MspI CCGG 7 cut(s) 44, 157, 169, 187, 247, 278, 308
MspR9I CCNGG 5 cut(s) 65, 110, 158, 248, 278
MvaI CCWGG 2 cut(s) 65, 110
MvnI CGCG 2 cut(s) 154, 164
MwoI GCNNNNNNNGC 7 cut(s) 40, 80, 105, 151, 158, 198, 299
NciI CCSGG 3 cut(s) 158, 248, 278
NdeII GATC 1 cut(s) 499
NlaIII CATG 1 cut(s) 307
NlaIV GGNNCC 4 cut(s) 100, 133, 191, 276
NmeAIII GCCGAG 2 cut(s) 75, 128
NmuCI GTSAC 2 cut(s) 67, 205
PalAI GGCGCGCC 1 cut(s) 152
PauI GCGCGC 1 cut(s) 152
PciSI GCTCTTC 1 cut(s) 81
PcsI WCGNNNNNNNCGW 1 cut(s) 215
PdmI GAANNNNTTC 1 cut(s) 119
PfeI GAWTC 1 cut(s) 115
PkrI GCNGC 7 cut(s) 42, 52, 55, 163, 301, 325, 606
PleI GAGTC 1 cut(s) 337
PpsI GAGTC 1 cut(s) 337
Psp124BI GAGCTC 1 cut(s) 147
Psp6I CCWGG 2 cut(s) 63, 108
PspEI GGTNACC 1 cut(s) 67
PspFI CCCAGC 1 cut(s) 596
PspGI CCWGG 2 cut(s) 63, 108
PspN4I GGNNCC 4 cut(s) 100, 133, 191, 276
PspPI GGNCC 2 cut(s) 159, 274
PteI GCGCGC 1 cut(s) 152
SacI GAGCTC 1 cut(s) 147
SalI GTCGAC 1 cut(s) 233
SapI GCTCTTC 1 cut(s) 81
SatI GCNGC 7 cut(s) 41, 51, 54, 162, 300, 324, 605
Sau3AI GATC 1 cut(s) 499
Sau96I GGNCC 2 cut(s) 159, 274
SchI GAGTC 1 cut(s) 338
ScrFI CCNGG 5 cut(s) 65, 110, 158, 248, 278
SduI GDGCHC 2 cut(s) 147, 194
SgsI GGCGCGCC 1 cut(s) 152
SinI GGWCC 1 cut(s) 274
SmlI CTYRAG 2 cut(s) 524, 567
SmoI CTYRAG 2 cut(s) 524, 567
Sse9I AATT 1 cut(s) 434
SspMI CTAG 2 cut(s) 80, 333
SstI GAGCTC 1 cut(s) 147
StyD4I CCNGG 5 cut(s) 63, 108, 156, 246, 276
TaqI TCGA 4 cut(s) 24, 126, 234, 592
TasI AATT 1 cut(s) 434
TauI GCSGC 5 cut(s) 53, 56, 164, 302, 326
TfiI GAWTC 1 cut(s) 115
TseFI GTSAC 2 cut(s) 67, 205
TseI GCWGC 2 cut(s) 40, 604
Tsp45I GTSAC 2 cut(s) 67, 205
VpaK11BI GGWCC 1 cut(s) 274
XagI CCTNNNNNAGG 1 cut(s) 17
XcmI CCANNNNNNNNNTGG 1 cut(s) 13
XmiI GTMKAC 1 cut(s) 234
XmnI GAANNNNTTC 1 cut(s) 119
XspI CTAG 2 cut(s) 80, 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.