MD02G1018700.v1.1

Triphosphate tunel metalloenzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0002663
Physical Location & Seq
Reverse (-)
1370726 .. 1372915
2190 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1018700.v1.1.491

Sequence Viewer

Length: 822 bp
ATGTCTCAAATCCCCAAAGCTCTGATGGAAGTCGAGGTCAAGCTCAGGCTCCTCGACGCTGCCGCTCACCGCCAAGTCACCACCCTCCTGGCGCCATTCCTCGTCGCCACGCACCGCCAAGAGAATTTCTTTTTCGACGGCTCCAAAGCCGAGCTCTCGGCCCGCCTAGCCGTTCTCCGGCTACGATTCTCCGACAGGAGTCCTCTCTGCGCCGTGACGCTCAAGGCGCGGGCGGTGCTTGTCGATGGCATTAGCCGAGTGGAGGAGGACGAGGAGGAGCTTGATCACTCGATCGGGCGGGCCTGCGTCGCCGAGCCGGATAAGCTGATGGCCGCGGAGTCTAGGGTTTTGAGGAGGGTGAGGGAGGAATTTGGGGTTTTAGGGTTTGTGGGATTGGGAGGATTTAGGAATGTGAGGGATGTGTATGATTGGAAGGGCTTGAAATTGGAGGTGGATGAGACTAAGTATGATTTTGGTACTTGTTACGAAATTGAGTGTGAGAGTGCTGACCCTGAAGGAGTGAAGGTGGTGCTTGAGGACTTTTTGAAGGAAAATGGGGTTCGGTATTCCTACTCCGAAGTGTCAAAGTTTGCGACTTTTCGAGCTGGGAAGCTGCCCGTTATTCAGCGGAGCGCAATTGGAGTCTCAGTCGGGAGAAATTCTGCGATGAATCAAAGGATGAGGACGTCTCCAAATCCTCGAGATCACTGTACTCTGCTTCTGTTCCGAACTTCATCTTCTCATTCTATTGTCATAGAACTCCGATACTTTCTTAACTCAAGGACGTCGGTAAATTCCATTGTTTGTAATAAATTGCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CYTH

Protein Analysis

274

Amino Acids

30.75

Weight (kDa)

8.19

Isoelectric Point (pI)

43.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CYTH PF01928 9 - 197 4e-20 CYTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 689, 788
AccB1I GGYRCC 1 cut(s) 91
AccBSI CCGCTC 1 cut(s) 65
AccII CGCG 2 cut(s) 229, 335
AcoI YGGCCR 1 cut(s) 330
AcsI RAATTY 4 cut(s) 124, 368, 658, 793
AcuI CTGAAG 1 cut(s) 534
AcyI GRCGYC 3 cut(s) 92, 686, 785
AfaI GTAC 2 cut(s) 478, 712
AfiI CCNNNNNNNGG 2 cut(s) 177, 262
AgsI TTSAA 2 cut(s) 442, 547
AjnI CCWGG 1 cut(s) 87
AluBI AGCT 7 cut(s) 20, 43, 154, 280, 325, 605, 613
AluI AGCT 7 cut(s) 20, 43, 154, 280, 325, 605, 613
Alw21I GWGCWC 1 cut(s) 156
Alw26I GTCTC 4 cut(s) 9, 452, 649, 693
Ama87I CYCGRG 1 cut(s) 699
AoxI GGCC 3 cut(s) 159, 300, 330
ApeKI GCWGC 2 cut(s) 59, 613
ApoI RAATTY 4 cut(s) 124, 368, 658, 793
AspLEI GCGC 4 cut(s) 94, 212, 229, 635
AspS9I GGNCC 2 cut(s) 160, 300
AsuHPI GGTGA 3 cut(s) 59, 70, 370
AvaI CYCGRG 1 cut(s) 699
BanI GGYRCC 1 cut(s) 91
BanII GRGCYC 1 cut(s) 156
Bbv12I GWGCWC 1 cut(s) 156
BbvI GCAGC 2 cut(s) 46, 600
BccI CCATC 3 cut(s) 19, 239, 322
BceAI ACGGC 3 cut(s) 154, 155, 197
BciT130I CCWGG 1 cut(s) 89
BclI TGATCA 1 cut(s) 283
BcoDI GTCTC 4 cut(s) 9, 452, 649, 693
BfaI CTAG 2 cut(s) 167, 342
BfoI RGCGCY 1 cut(s) 95
BisI GCNGC 4 cut(s) 60, 63, 333, 614
BlsI GCNGC 4 cut(s) 61, 64, 334, 615
Bme1390I CCNGG 1 cut(s) 89
BmeT110I CYCGRG 1 cut(s) 699
BmgT120I GGNCC 2 cut(s) 160, 300
BmiI GGNNCC 3 cut(s) 50, 93, 142
BmrFI CCNGG 1 cut(s) 89
BoxI GACNNNNGTC 1 cut(s) 198
BplI GAGNNNNNCTC 2 cut(s) 673, 705
Bpu10I CCTNAGC 1 cut(s) 44
BpuEI CTTGAG 3 cut(s) 206, 554, 763
BsaHI GRCGYC 3 cut(s) 92, 686, 785
BsaJI CCNNGG 1 cut(s) 333
BsaXI ACNNNNNCTCC 4 cut(s) 510, 540, 633, 663
Bsc4I CCNNNNNNNGG 2 cut(s) 177, 262
BseBI CCWGG 1 cut(s) 89
BseDI CCNNGG 1 cut(s) 333
BseGI GGATG 3 cut(s) 424, 460, 684
BseLI CCNNNNNNNGG 2 cut(s) 177, 262
BseMII CTCAG 2 cut(s) 58, 660
BseRI GAGGAG 5 cut(s) 41, 278, 287, 290, 367
BseXI GCAGC 2 cut(s) 46, 600
BseYI CCCAGC 1 cut(s) 605
Bsh1236I CGCG 2 cut(s) 229, 335
Bsh1285I CGRYCG 1 cut(s) 294
BshFI GGCC 3 cut(s) 161, 302, 332
BshNI GGYRCC 1 cut(s) 91
BsiEI CGRYCG 1 cut(s) 294
BsiHKAI GWGCWC 1 cut(s) 156
BsiHKCI CYCGRG 1 cut(s) 699
BsiSI CCGG 2 cut(s) 178, 317
BslI CCNNNNNNNGG 2 cut(s) 177, 262
BsmAI GTCTC 4 cut(s) 9, 452, 649, 693
BsmBI CGTCTC 1 cut(s) 693
BsnI GGCC 3 cut(s) 161, 302, 332
BsoBI CYCGRG 1 cut(s) 699
Bsp1286I GDGCHC 1 cut(s) 156
Bsp143I GATC 3 cut(s) 283, 291, 703
BspANI GGCC 3 cut(s) 161, 302, 332
BspCNI CTCAG 2 cut(s) 57, 659
BspFNI CGCG 2 cut(s) 229, 335
BspLI GGNNCC 3 cut(s) 50, 93, 142
BspT107I GGYRCC 1 cut(s) 91
BsrBI CCGCTC 1 cut(s) 65
BssECI CCNNGG 1 cut(s) 333
BssMI GATC 3 cut(s) 283, 291, 703
BssNI GRCGYC 3 cut(s) 92, 686, 785
Bst2UI CCWGG 1 cut(s) 89
Bst4CI ACNGT 1 cut(s) 710
BstACI GRCGYC 3 cut(s) 92, 686, 785
BstC8I GCNNGC 4 cut(s) 163, 231, 300, 304
BstDEI CTNAG 3 cut(s) 44, 462, 646
BstDSI CCRYGG 1 cut(s) 333
BstF5I GGATG 3 cut(s) 424, 460, 684
BstFNI CGCG 2 cut(s) 229, 335
BstH2I RGCGCY 1 cut(s) 95
BstHHI GCGC 4 cut(s) 94, 212, 229, 635
BstKTI GATC 3 cut(s) 286, 294, 706
BstMAI GTCTC 4 cut(s) 9, 452, 649, 693
BstMBI GATC 3 cut(s) 283, 291, 703
BstMCI CGRYCG 1 cut(s) 294
BstMWI GCNNNNNNNGC 5 cut(s) 167, 226, 235, 308, 322
BstNI CCWGG 1 cut(s) 89
BstPAI GACNNNNGTC 1 cut(s) 198
BstSCI CCNGG 1 cut(s) 87
BstUI CGCG 2 cut(s) 229, 335
BstV1I GCAGC 2 cut(s) 46, 600
BstXI CCANNNNNNTGG 1 cut(s) 88
BsuRI GGCC 3 cut(s) 161, 302, 332
BtgI CCRYGG 1 cut(s) 333
BtgZI GCGATG 1 cut(s) 680
BtsCI GGATG 3 cut(s) 424, 460, 684
BtsIMutI CAGTG 1 cut(s) 706
Cac8I GCNNGC 4 cut(s) 163, 231, 300, 304
CfoI GCGC 4 cut(s) 94, 212, 229, 635
Cfr13I GGNCC 2 cut(s) 160, 300
Cfr42I CCGCGG 1 cut(s) 336
CseI GACGC 3 cut(s) 65, 226, 295
Csp6I GTAC 2 cut(s) 477, 711
CviQI GTAC 2 cut(s) 477, 711
DdeI CTNAG 3 cut(s) 44, 462, 646
DinI GGCGCC 1 cut(s) 93
DpnI GATC 3 cut(s) 285, 293, 705
DpnII GATC 3 cut(s) 283, 291, 703
EaeI YGGCCR 1 cut(s) 330
Ecl136II GAGCTC 1 cut(s) 154
Eco24I GRGCYC 1 cut(s) 156
Eco53kI GAGCTC 1 cut(s) 154
Eco57I CTGAAG 1 cut(s) 534
Eco88I CYCGRG 1 cut(s) 699
EcoICRI GAGCTC 1 cut(s) 154
EcoRII CCWGG 1 cut(s) 87
EcoT38I GRGCYC 1 cut(s) 156
EgeI GGCGCC 1 cut(s) 93
EheI GGCGCC 1 cut(s) 93
Esp3I CGTCTC 1 cut(s) 693
FaiI YATR 3 cut(s) 426, 468, 755
FauI CCCGC 3 cut(s) 170, 222, 291
FbaI TGATCA 1 cut(s) 283
Fnu4HI GCNGC 4 cut(s) 60, 63, 333, 614
FokI GGATG 3 cut(s) 431, 467, 691
FriOI GRGCYC 1 cut(s) 156
Fsp4HI GCNGC 4 cut(s) 60, 63, 333, 614
FspBI CTAG 2 cut(s) 167, 342
GlaI GCGC 4 cut(s) 93, 211, 228, 634
GluI GCNGC 4 cut(s) 60, 63, 333, 614
GsaI CCCAGC 1 cut(s) 609
HaeII RGCGCY 1 cut(s) 95
HaeIII GGCC 3 cut(s) 161, 302, 332
HapII CCGG 2 cut(s) 178, 317
HgaI GACGC 3 cut(s) 65, 226, 295
HhaI GCGC 4 cut(s) 94, 212, 229, 635
Hin1I GRCGYC 3 cut(s) 92, 686, 785
Hin6I GCGC 4 cut(s) 92, 210, 227, 633
HinP1I GCGC 4 cut(s) 92, 210, 227, 633
HinfI GANTC 5 cut(s) 186, 199, 338, 642, 670
HpaII CCGG 2 cut(s) 178, 317
HphI GGTGA 3 cut(s) 59, 70, 370
Hpy188I TCNGA 5 cut(s) 24, 193, 577, 728, 764
Hpy188III TCNNGA 2 cut(s) 652, 701
Hpy99I CGWCG 5 cut(s) 59, 107, 140, 311, 790
HpyAV CCTTC 4 cut(s) 427, 509, 517, 541
HpyCH4III ACNGT 1 cut(s) 710
HpyCH4IV ACGT 2 cut(s) 686, 785
HpyCH4V TGCA 1 cut(s) 817
HpyF10VI GCNNNNNNNGC 5 cut(s) 167, 226, 235, 308, 322
HpyF3I CTNAG 3 cut(s) 44, 462, 646
HpySE526I ACGT 2 cut(s) 686, 785
Hsp92I GRCGYC 3 cut(s) 92, 686, 785
HspAI GCGC 4 cut(s) 92, 210, 227, 633
KasI GGCGCC 1 cut(s) 91
Ksp22I TGATCA 1 cut(s) 283
KspI CCGCGG 1 cut(s) 336
Kzo9I GATC 3 cut(s) 283, 291, 703
LmnI GCTCC 4 cut(s) 54, 146, 277, 630
LpnPI CCDG 9 cut(s) 31, 74, 101, 181, 191, 316, 330, 525, 591
Lsp1109I GCAGC 2 cut(s) 46, 600
MaeI CTAG 2 cut(s) 167, 342
MaeII ACGT 2 cut(s) 686, 785
MaeIII GTNAC 3 cut(s) 76, 214, 482
MalI GATC 3 cut(s) 285, 293, 705
MbiI CCGCTC 1 cut(s) 65
MboI GATC 3 cut(s) 283, 291, 703
MboII GAAGA 1 cut(s) 729
MfeI CAATTG 1 cut(s) 636
MhlI GDGCHC 1 cut(s) 156
MluCI AATT 8 cut(s) 124, 368, 443, 489, 636, 658, 793, 812
Mly113I GGCGCC 1 cut(s) 92
MlyI GAGTC 3 cut(s) 208, 347, 651
MmeI TCCRAC 1 cut(s) 216
MseI TTAA 1 cut(s) 774
MspA1I CMGCKG 2 cut(s) 335, 628
MspI CCGG 2 cut(s) 178, 317
MspR9I CCNGG 1 cut(s) 89
MunI CAATTG 1 cut(s) 636
MvaI CCWGG 1 cut(s) 89
MvnI CGCG 2 cut(s) 229, 335
MwoI GCNNNNNNNGC 5 cut(s) 167, 226, 235, 308, 322
NarI GGCGCC 1 cut(s) 92
NdeII GATC 3 cut(s) 283, 291, 703
NlaIV GGNNCC 3 cut(s) 50, 93, 142
NmeAIII GCCGAG 4 cut(s) 137, 175, 281, 337
NmuCI GTSAC 2 cut(s) 76, 214
PaeR7I CTCGAG 1 cut(s) 699
PfeI GAWTC 2 cut(s) 186, 670
PkrI GCNGC 4 cut(s) 61, 64, 334, 615
Ple19I CGATCG 1 cut(s) 294
PleI GAGTC 3 cut(s) 207, 346, 650
PluTI GGCGCC 1 cut(s) 95
PpsI GAGTC 3 cut(s) 207, 346, 650
PshAI GACNNNNGTC 1 cut(s) 198
Psp124BI GAGCTC 1 cut(s) 156
Psp6I CCWGG 1 cut(s) 87
PspFI CCCAGC 1 cut(s) 605
PspGI CCWGG 1 cut(s) 87
PspN4I GGNNCC 3 cut(s) 50, 93, 142
PspPI GGNCC 2 cut(s) 160, 300
PvuI CGATCG 1 cut(s) 294
RsaI GTAC 2 cut(s) 478, 712
RsaNI GTAC 2 cut(s) 477, 711
SacI GAGCTC 1 cut(s) 156
SacII CCGCGG 1 cut(s) 336
SaqAI TTAA 1 cut(s) 774
SatI GCNGC 4 cut(s) 60, 63, 333, 614
Sau3AI GATC 3 cut(s) 283, 291, 703
Sau96I GGNCC 2 cut(s) 160, 300
SchI GAGTC 3 cut(s) 208, 347, 651
ScrFI CCNGG 1 cut(s) 89
SduI GDGCHC 1 cut(s) 156
SfoI GGCGCC 1 cut(s) 93
Sfr274I CTCGAG 1 cut(s) 699
Sfr303I CCGCGG 1 cut(s) 336
SgrBI CCGCGG 1 cut(s) 336
SlaI CTCGAG 1 cut(s) 699
SmlI CTYRAG 4 cut(s) 221, 533, 699, 778
SmoI CTYRAG 4 cut(s) 221, 533, 699, 778
Sse9I AATT 8 cut(s) 124, 368, 443, 489, 636, 658, 793, 812
SspDI GGCGCC 1 cut(s) 91
SspMI CTAG 2 cut(s) 167, 342
SstI GAGCTC 1 cut(s) 156
StyD4I CCNGG 1 cut(s) 87
TaaI ACNGT 1 cut(s) 710
TaiI ACGT 2 cut(s) 689, 788
TaqI TCGA 7 cut(s) 33, 54, 135, 243, 290, 601, 700
TasI AATT 8 cut(s) 124, 368, 443, 489, 636, 658, 793, 812
TatI WGTACW 1 cut(s) 710
TauI GCSGC 2 cut(s) 65, 335
TfiI GAWTC 2 cut(s) 186, 670
Tru1I TTAA 1 cut(s) 774
Tru9I TTAA 1 cut(s) 774
TscAI CASTG 1 cut(s) 713
TseFI GTSAC 2 cut(s) 76, 214
TseI GCWGC 2 cut(s) 59, 613
Tsp45I GTSAC 2 cut(s) 76, 214
TspDTI ATGAA 2 cut(s) 683, 723
TspRI CASTG 1 cut(s) 713
XapI RAATTY 4 cut(s) 124, 368, 658, 793
XcmI CCANNNNNNNNNTGG 1 cut(s) 22
XhoI CTCGAG 1 cut(s) 699
XspI CTAG 2 cut(s) 167, 342
ZraI GACGTC 2 cut(s) 687, 786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.