Rroxscaffold_2G00153790

Triphosphate tunel metalloenzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002663
Physical Location & Seq
Forward (+)
90508398 .. 90509199
802 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00153790.1

Sequence Viewer

Length: 687 bp
ATGCTGAGACGAAAGCCAACCAAGATCGAACTCAATATCGAAGACAAAGAAGAACTCGAAGAAGCTCGTCAACGCGCCGCCGCCGCCACAGCCACCACCGCCGCCTCCACCGCTGCGGACGCCGCCGCCCACGGCCAGGTCACCACTCTTTCTAGCGCCGTTCCACCCTCGGGCACCCACCGCCAGGGAGAATCTCTTCTTCGACGGCTCCAAAATCCGAGCTCTCGGCGCGCCGGCCGTGCTCCGGCTCCGGTTCTCCGACCGGAGCCCCTCTGCGCCGTGACGCTGAAGGCGGCGGGCGGTGCTGGTCGACGGCGTGAGCCGGTGGAGGAGGACGAGGAGGAGATGGACCCGGCGGTGGGGCGTGCGCGCGCGCCGAGCCGGGAGAAGATAATGGCGGCGGAGTCTAGGGTTTTAACGAGGGTGAGGGAGGAGTTTGGGGTTTTAGGGTTTGTGGGGATGGGAGGGTTTGGGAATGTGAGGGATGTGTATGATTGGAAAGGGTTGAAATTGGAGGTGGATGAGACTAAGTATGAGTTTGGGACTTGTTATGAGATTGAGTGTGAGAGCGCTGATCCTGATGGGGCGAAGGAGAAGCTTGAGGAGTTCTTGAAGGAAAATGGGGTGAGGTATTCCGATTCTCAAGTGTCCAAGTTTGCGACTTTTCGAGCTGGGAAGCTGCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CYTH

Protein Analysis

228

Amino Acids

24.91

Weight (kDa)

5.93

Isoelectric Point (pI)

62.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANAPC_CDC26 PF10471 1 - 54 1.4e-09 Anaphase-promoting complex APC subunit CDC26
CYTH PF01928 95 - 208 3.9e-09 CYTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 173
AccI GTMKAC 1 cut(s) 310
AccII CGCG 5 cut(s) 75, 231, 370, 372, 374
AclWI GGATC 1 cut(s) 569
AcoI YGGCCR 2 cut(s) 133, 235
AcuI CTGAAG 1 cut(s) 308
AcyI GRCGYC 1 cut(s) 120
AfeI AGCGCT 1 cut(s) 571
AfiI CCNNNNNNNGG 6 cut(s) 136, 170, 184, 244, 358, 359
AgsI TTSAA 2 cut(s) 508, 613
AjnI CCWGG 2 cut(s) 135, 183
AluBI AGCT 5 cut(s) 65, 222, 598, 671, 679
AluI AGCT 5 cut(s) 65, 222, 598, 671, 679
Alw21I GWGCWC 2 cut(s) 224, 244
Alw26I GTCTC 1 cut(s) 518
AlwI GGATC 1 cut(s) 569
Ama87I CYCGRG 1 cut(s) 169
Aor51HI AGCGCT 1 cut(s) 571
AoxI GGCC 2 cut(s) 133, 235
ApeKI GCWGC 2 cut(s) 113, 679
AscI GGCGCGCC 1 cut(s) 229
Asp700I GAANNNNTTC 1 cut(s) 195
AspS9I GGNCC 1 cut(s) 349
AsuC2I CCSGG 2 cut(s) 353, 383
AsuHPI GGTGA 3 cut(s) 133, 436, 637
AvaI CYCGRG 1 cut(s) 169
AvaII GGWCC 1 cut(s) 349
BaeGI GKGCMC 1 cut(s) 176
BanI GGYRCC 1 cut(s) 173
BanII GRGCYC 2 cut(s) 224, 270
BbsI GAAGAC 1 cut(s) 48
Bbv12I GWGCWC 2 cut(s) 224, 244
BbvI GCAGC 2 cut(s) 100, 666
BccI CCATC 3 cut(s) 340, 454, 575
BceAI ACGGC 6 cut(s) 143, 148, 221, 222, 263, 329
BciT130I CCWGG 2 cut(s) 137, 185
BcnI CCSGG 2 cut(s) 353, 383
BcoDI GTCTC 1 cut(s) 518
BfaI CTAG 2 cut(s) 153, 408
BfoI RGCGCY 2 cut(s) 159, 573
Bme1390I CCNGG 4 cut(s) 137, 185, 353, 383
Bme18I GGWCC 1 cut(s) 349
BmeT110I CYCGRG 1 cut(s) 169
BmgT120I GGNCC 1 cut(s) 349
BmiI GGNNCC 5 cut(s) 175, 209, 249, 267, 351
BmrFI CCNGG 4 cut(s) 137, 185, 353, 383
BpiI GAAGAC 1 cut(s) 48
BpuEI CTTGAG 2 cut(s) 620, 627
BpuMI CCSGG 2 cut(s) 353, 383
BsaHI GRCGYC 1 cut(s) 120
BsaJI CCNNGG 3 cut(s) 130, 168, 184
BsaWI WCCGGW 2 cut(s) 250, 262
Bsc4I CCNNNNNNNGG 6 cut(s) 136, 170, 184, 244, 358, 359
Bse118I RCCGGY 2 cut(s) 233, 322
BseBI CCWGG 2 cut(s) 137, 185
BseDI CCNNGG 3 cut(s) 130, 168, 184
BseGI GGATG 3 cut(s) 465, 490, 526
BseLI CCNNNNNNNGG 6 cut(s) 136, 170, 184, 244, 358, 359
BsePI GCGCGC 4 cut(s) 229, 368, 370, 372
BseRI GAGGAG 5 cut(s) 344, 353, 356, 446, 617
BseSI GKGCMC 1 cut(s) 176
BseX3I CGGCCG 1 cut(s) 235
BseXI GCAGC 2 cut(s) 100, 666
BseYI CCCAGC 1 cut(s) 671
Bsh1236I CGCG 5 cut(s) 75, 231, 370, 372, 374
Bsh1285I CGRYCG 2 cut(s) 238, 263
BshFI GGCC 2 cut(s) 135, 237
BshNI GGYRCC 1 cut(s) 173
BsiEI CGRYCG 2 cut(s) 238, 263
BsiHKAI GWGCWC 2 cut(s) 224, 244
BsiHKCI CYCGRG 1 cut(s) 169
BsiSI CCGG 7 cut(s) 234, 245, 251, 263, 323, 353, 382
BslFI GGGAC 1 cut(s) 556
BslI CCNNNNNNNGG 6 cut(s) 136, 170, 184, 244, 358, 359
BsmAI GTCTC 1 cut(s) 518
BsmFI GGGAC 1 cut(s) 556
BsnI GGCC 2 cut(s) 135, 237
BsoBI CYCGRG 1 cut(s) 169
Bsp1286I GDGCHC 4 cut(s) 176, 224, 244, 270
Bsp143I GATC 2 cut(s) 24, 574
BspANI GGCC 2 cut(s) 135, 237
BspFNI CGCG 5 cut(s) 75, 231, 370, 372, 374
BspLI GGNNCC 5 cut(s) 175, 209, 249, 267, 351
BspPI GGATC 1 cut(s) 569
BspT107I GGYRCC 1 cut(s) 173
BsrFI RCCGGY 2 cut(s) 233, 322
BssAI RCCGGY 2 cut(s) 233, 322
BssECI CCNNGG 3 cut(s) 130, 168, 184
BssHII GCGCGC 4 cut(s) 229, 368, 370, 372
BssMI GATC 2 cut(s) 24, 574
BssNI GRCGYC 1 cut(s) 120
Bst2UI CCWGG 2 cut(s) 137, 185
Bst6I CTCTTC 1 cut(s) 201
BstACI GRCGYC 1 cut(s) 120
BstC8I GCNNGC 7 cut(s) 231, 235, 298, 366, 370, 372, 374
BstDEI CTNAG 3 cut(s) 5, 528, 684
BstDSI CCRYGG 1 cut(s) 130
BstEII GGTNACC 1 cut(s) 139
BstF5I GGATG 3 cut(s) 465, 490, 526
BstFNI CGCG 5 cut(s) 75, 231, 370, 372, 374
BstH2I RGCGCY 2 cut(s) 159, 573
BstKTI GATC 2 cut(s) 27, 577
BstMAI GTCTC 1 cut(s) 518
BstMBI GATC 2 cut(s) 24, 574
BstMCI CGRYCG 2 cut(s) 238, 263
BstNI CCWGG 2 cut(s) 137, 185
BstPI GGTNACC 1 cut(s) 139
BstSCI CCNGG 4 cut(s) 135, 183, 351, 381
BstSLI GKGCMC 1 cut(s) 176
BstUI CGCG 5 cut(s) 75, 231, 370, 372, 374
BstV1I GCAGC 2 cut(s) 100, 666
BstV2I GAAGAC 1 cut(s) 48
BstZI CGGCCG 1 cut(s) 235
BsuRI GGCC 2 cut(s) 135, 237
BtgI CCRYGG 1 cut(s) 130
BtsCI GGATG 3 cut(s) 465, 490, 526
Cac8I GCNNGC 7 cut(s) 231, 235, 298, 366, 370, 372, 374
Cfr10I RCCGGY 2 cut(s) 233, 322
Cfr13I GGNCC 1 cut(s) 349
CseI GACGC 2 cut(s) 128, 292
DdeI CTNAG 3 cut(s) 5, 528, 684
DpnI GATC 2 cut(s) 26, 576
DpnII GATC 2 cut(s) 24, 574
EaeI YGGCCR 2 cut(s) 133, 235
EagI CGGCCG 1 cut(s) 235
Eam1104I CTCTTC 1 cut(s) 201
EarI CTCTTC 1 cut(s) 201
EciI GGCGGA 1 cut(s) 416
Ecl136II GAGCTC 1 cut(s) 222
EclXI CGGCCG 1 cut(s) 235
Eco24I GRGCYC 2 cut(s) 224, 270
Eco47I GGWCC 1 cut(s) 349
Eco47III AGCGCT 1 cut(s) 571
Eco52I CGGCCG 1 cut(s) 235
Eco53kI GAGCTC 1 cut(s) 222
Eco57I CTGAAG 1 cut(s) 308
Eco88I CYCGRG 1 cut(s) 169
Eco91I GGTNACC 1 cut(s) 139
EcoICRI GAGCTC 1 cut(s) 222
EcoO65I GGTNACC 1 cut(s) 139
EcoRII CCWGG 2 cut(s) 135, 183
EcoT38I GRGCYC 2 cut(s) 224, 270
FaiI YATR 3 cut(s) 492, 534, 552
FaqI GGGAC 1 cut(s) 556
FauI CCCGC 1 cut(s) 289
FblI GTMKAC 1 cut(s) 310
FokI GGATG 3 cut(s) 472, 497, 533
FriOI GRGCYC 2 cut(s) 224, 270
FspBI CTAG 2 cut(s) 153, 408
GsaI CCCAGC 1 cut(s) 675
HaeII RGCGCY 2 cut(s) 159, 573
HaeIII GGCC 2 cut(s) 135, 237
HapII CCGG 7 cut(s) 234, 245, 251, 263, 323, 353, 382
HgaI GACGC 2 cut(s) 128, 292
Hin1I GRCGYC 1 cut(s) 120
HincII GTYRAC 2 cut(s) 71, 311
HindII GTYRAC 2 cut(s) 71, 311
HindIII AAGCTT 1 cut(s) 596
HinfI GANTC 3 cut(s) 191, 404, 638
HpaII CCGG 7 cut(s) 234, 245, 251, 263, 323, 353, 382
HphI GGTGA 3 cut(s) 133, 436, 637
Hpy166II GTNNAC 2 cut(s) 71, 311
Hpy188I TCNGA 3 cut(s) 219, 260, 637
Hpy188III TCNNGA 2 cut(s) 578, 610
Hpy8I GTNNAC 2 cut(s) 71, 311
Hpy99I CGWCG 2 cut(s) 207, 315
HpyAV CCTTC 3 cut(s) 283, 583, 607
HpyF3I CTNAG 3 cut(s) 5, 528, 684
Hsp92I GRCGYC 1 cut(s) 120
KroI GCCGGC 1 cut(s) 233
KroNI GCCGGC 1 cut(s) 235
Kzo9I GATC 2 cut(s) 24, 574
LmnI GCTCC 4 cut(s) 213, 247, 253, 265
Lsp1109I GCAGC 2 cut(s) 100, 666
MaeI CTAG 2 cut(s) 153, 408
MaeIII GTNAC 2 cut(s) 139, 280
MalI GATC 2 cut(s) 26, 576
MauBI CGCGCGCG 1 cut(s) 370
MboI GATC 2 cut(s) 24, 574
MboII GAAGA 6 cut(s) 53, 62, 71, 188, 191, 400
MhlI GDGCHC 4 cut(s) 176, 224, 244, 270
MluCI AATT 1 cut(s) 509
MlyI GAGTC 1 cut(s) 413
MmeI TCCRAC 1 cut(s) 283
MroNI GCCGGC 1 cut(s) 233
MroXI GAANNNNTTC 1 cut(s) 195
MseI TTAA 1 cut(s) 416
MspA1I CMGCKG 1 cut(s) 113
MspI CCGG 7 cut(s) 234, 245, 251, 263, 323, 353, 382
MspR9I CCNGG 4 cut(s) 137, 185, 353, 383
MvaI CCWGG 2 cut(s) 137, 185
MvnI CGCG 5 cut(s) 75, 231, 370, 372, 374
NaeI GCCGGC 1 cut(s) 235
NciI CCSGG 2 cut(s) 353, 383
NdeII GATC 2 cut(s) 24, 574
NgoMIV GCCGGC 1 cut(s) 233
NlaIV GGNNCC 5 cut(s) 175, 209, 249, 267, 351
NmeAIII GCCGAG 2 cut(s) 205, 402
NmuCI GTSAC 2 cut(s) 139, 280
PalAI GGCGCGCC 1 cut(s) 229
PauI GCGCGC 4 cut(s) 229, 368, 370, 372
PdiI GCCGGC 1 cut(s) 235
PdmI GAANNNNTTC 1 cut(s) 195
PfeI GAWTC 2 cut(s) 191, 638
PleI GAGTC 1 cut(s) 412
PpsI GAGTC 1 cut(s) 412
Psp124BI GAGCTC 1 cut(s) 224
Psp6I CCWGG 2 cut(s) 135, 183
PspEI GGTNACC 1 cut(s) 139
PspFI CCCAGC 1 cut(s) 671
PspGI CCWGG 2 cut(s) 135, 183
PspN4I GGNNCC 5 cut(s) 175, 209, 249, 267, 351
PspPI GGNCC 1 cut(s) 349
PteI GCGCGC 4 cut(s) 229, 368, 370, 372
SacI GAGCTC 1 cut(s) 224
SalI GTCGAC 1 cut(s) 309
SaqAI TTAA 1 cut(s) 416
Sau3AI GATC 2 cut(s) 24, 574
Sau96I GGNCC 1 cut(s) 349
SchI GAGTC 1 cut(s) 413
ScrFI CCNGG 4 cut(s) 137, 185, 353, 383
SduI GDGCHC 4 cut(s) 176, 224, 244, 270
SetI ASST 8 cut(s) 67, 141, 224, 519, 600, 632, 673, 681
SgsI GGCGCGCC 1 cut(s) 229
SinI GGWCC 1 cut(s) 349
SmlI CTYRAG 2 cut(s) 599, 642
SmoI CTYRAG 2 cut(s) 599, 642
Sse9I AATT 1 cut(s) 509
SspMI CTAG 2 cut(s) 153, 408
SstI GAGCTC 1 cut(s) 224
StyD4I CCNGG 4 cut(s) 135, 183, 351, 381
TaqI TCGA 6 cut(s) 27, 39, 57, 202, 310, 667
TasI AATT 1 cut(s) 509
TauI GCSGC 8 cut(s) 80, 83, 86, 104, 125, 128, 296, 401
TfiI GAWTC 2 cut(s) 191, 638
Tru1I TTAA 1 cut(s) 416
Tru9I TTAA 1 cut(s) 416
TseFI GTSAC 2 cut(s) 139, 280
TseI GCWGC 2 cut(s) 113, 679
Tsp45I GTSAC 2 cut(s) 139, 280
VpaK11BI GGWCC 1 cut(s) 349
XmiI GTMKAC 1 cut(s) 310
XmnI GAANNNNTTC 1 cut(s) 195
XspI CTAG 2 cut(s) 153, 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.