MD15G1162700.v1.1

Triphosphate tunel metalloenzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002663
Physical Location & Seq
Reverse (-)
12255667 .. 12259815
4149 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1162700.v1.1.491

Sequence Viewer

Length: 753 bp
ATGTCTCAAATCCCCAAAGCTCTGATGGAAGTGGAGGTCAAGCTCCGGCTCCTGGACGCCGCCGCCCACCGCCAAGTCACCACCCTCCTGGCGCCATTCCTCGTCGCCACACACCGCCAGGAGAATCTCTTCTTCGACGGCTCCAAAGCCGAGCTCGCGGCGCGCCGGGCCGTCCTCCGGCTACGATTCTCCGACAGGAGCCCTCTCTGTGCCGTGACGCTCAAGGCGCGGGCAGTGCTGGTCCAAGGCGTCAGCCGAGTTGAGGAGGACGAGGAGGAGCTCGACCATTCGATCGGGCGGGCCTGCGCTGCCGAGCCAGAGAAGCTGATGACGGCGGAGTCTAGGGTTCTGAGGAGGGTGAGAGAGGAATTTGGGGTTTTAGGGTTTGTGGGATTGGGAGGGTTCAGGAATGTGAGGGATGTGTATGATTGGAAAGGCTTGAAATTGGAGGTGGATGAGACTAAGTATGATTTTGGTACTTGTTATGAGATTGAGTGTGAGAGTGCTGACCCTGAAGGAGTCAAGGTGGTGCTTGAGGACTTGTTGAAGGAAAATGGGGTTCGGTATTCTTACTCAGAAGTATCAAAGTTTGCAATTTTTCGAGCTGGGAAGCTGCCCGTTACCGAACCCTCTTCTTCTCTATCCCAAGAGACCCACGATCCCCAAACTCCATTAAGGACAAGAAGATTGAGGACTCAATCGAGAGACCGCCATCCAGTGGTTGGGCAACAACTAGTTGTCGGATGCCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CYTH

Protein Analysis

251

Amino Acids

28.17

Weight (kDa)

6.98

Isoelectric Point (pI)

55.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CYTH PF01928 9 - 186 3.6e-21 CYTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 337
AccB1I GGYRCC 1 cut(s) 91
AccB7I CCANNNNNTGG 2 cut(s) 718, 722
AccII CGCG 3 cut(s) 158, 163, 229
AciI CCGC 9 cut(s) 60, 63, 70, 115, 158, 229, 298, 335, 709
AclWI GGATC 1 cut(s) 653
AcsI RAATTY 1 cut(s) 368
AcuI CTGAAG 1 cut(s) 534
AcyI GRCGYC 3 cut(s) 57, 92, 249
AfaI GTAC 1 cut(s) 478
AfiI CCNNNNNNNGG 5 cut(s) 52, 177, 262, 718, 722
AgsI TTSAA 2 cut(s) 442, 547
AhlI ACTAGT 1 cut(s) 733
AjnI CCWGG 3 cut(s) 51, 87, 117
AluBI AGCT 7 cut(s) 20, 43, 154, 280, 325, 605, 613
AluI AGCT 7 cut(s) 20, 43, 154, 280, 325, 605, 613
Alw21I GWGCWC 2 cut(s) 156, 282
Alw26I GTCTC 4 cut(s) 9, 452, 644, 699
AlwI GGATC 1 cut(s) 653
AoxI GGCC 2 cut(s) 168, 300
ApeKI GCWGC 2 cut(s) 308, 613
ApoI RAATTY 1 cut(s) 368
AscI GGCGCGCC 1 cut(s) 161
Asp700I GAANNNNTTC 1 cut(s) 128
AspLEI GCGC 5 cut(s) 94, 163, 165, 229, 308
AspS9I GGNCC 3 cut(s) 168, 241, 300
AsuC2I CCSGG 1 cut(s) 167
AsuHPI GGTGA 2 cut(s) 70, 370
AvaII GGWCC 1 cut(s) 241
BanI GGYRCC 1 cut(s) 91
BanII GRGCYC 3 cut(s) 156, 203, 282
Bbv12I GWGCWC 2 cut(s) 156, 282
BbvI GCAGC 2 cut(s) 295, 600
BccI CCATC 2 cut(s) 19, 720
BceAI ACGGC 4 cut(s) 154, 155, 197, 348
BciT130I CCWGG 3 cut(s) 53, 89, 119
BcnI CCSGG 1 cut(s) 167
BcoDI GTCTC 4 cut(s) 9, 452, 644, 699
BcuI ACTAGT 1 cut(s) 733
BfaI CTAG 2 cut(s) 342, 734
BfoI RGCGCY 1 cut(s) 95
BisI GCNGC 5 cut(s) 60, 63, 159, 309, 614
BlsI GCNGC 5 cut(s) 61, 64, 160, 310, 615
Bme1390I CCNGG 4 cut(s) 53, 89, 119, 167
Bme18I GGWCC 1 cut(s) 241
BmgT120I GGNCC 3 cut(s) 168, 241, 300
BmiI GGNNCC 4 cut(s) 50, 93, 142, 200
BmrFI CCNGG 4 cut(s) 53, 89, 119, 167
BmsI GCATC 1 cut(s) 734
BpuEI CTTGAG 2 cut(s) 206, 554
BpuMI CCSGG 1 cut(s) 167
BsaHI GRCGYC 3 cut(s) 57, 92, 249
BsaI GGTCTC 2 cut(s) 644, 699
BsaJI CCNNGG 1 cut(s) 244
BsaXI ACNNNNNCTCC 2 cut(s) 510, 540
Bsc4I CCNNNNNNNGG 5 cut(s) 52, 177, 262, 718, 722
Bse1I ACTGG 1 cut(s) 716
BseBI CCWGG 3 cut(s) 53, 89, 119
BseDI CCNNGG 1 cut(s) 244
BseGI GGATG 4 cut(s) 424, 460, 712, 749
BseLI CCNNNNNNNGG 5 cut(s) 52, 177, 262, 718, 722
BseMII CTCAG 2 cut(s) 341, 588
BseNI ACTGG 1 cut(s) 716
BsePI GCGCGC 1 cut(s) 161
BseRI GAGGAG 4 cut(s) 278, 287, 290, 367
BseXI GCAGC 2 cut(s) 295, 600
BseYI CCCAGC 1 cut(s) 605
Bsh1236I CGCG 3 cut(s) 158, 163, 229
Bsh1285I CGRYCG 1 cut(s) 294
BshFI GGCC 2 cut(s) 170, 302
BshNI GGYRCC 1 cut(s) 91
BsiEI CGRYCG 1 cut(s) 294
BsiHKAI GWGCWC 2 cut(s) 156, 282
BsiSI CCGG 3 cut(s) 46, 166, 178
BslI CCNNNNNNNGG 5 cut(s) 52, 177, 262, 718, 722
BsmAI GTCTC 4 cut(s) 9, 452, 644, 699
BsnI GGCC 2 cut(s) 170, 302
Bso31I GGTCTC 2 cut(s) 644, 699
Bsp1286I GDGCHC 3 cut(s) 156, 203, 282
Bsp143I GATC 2 cut(s) 291, 658
BspACI CCGC 9 cut(s) 60, 63, 70, 115, 158, 229, 298, 335, 709
BspANI GGCC 2 cut(s) 170, 302
BspCNI CTCAG 2 cut(s) 342, 587
BspFNI CGCG 3 cut(s) 158, 163, 229
BspLI GGNNCC 4 cut(s) 50, 93, 142, 200
BspPI GGATC 1 cut(s) 653
BspT107I GGYRCC 1 cut(s) 91
BspTNI GGTCTC 2 cut(s) 644, 699
BsrI ACTGG 1 cut(s) 716
BssECI CCNNGG 1 cut(s) 244
BssHII GCGCGC 1 cut(s) 161
BssMI GATC 2 cut(s) 291, 658
BssNI GRCGYC 3 cut(s) 57, 92, 249
BssT1I CCWWGG 1 cut(s) 244
Bst2UI CCWGG 3 cut(s) 53, 89, 119
Bst6I CTCTTC 2 cut(s) 134, 637
BstACI GRCGYC 3 cut(s) 57, 92, 249
BstC8I GCNNGC 5 cut(s) 156, 163, 231, 300, 304
BstDEI CTNAG 3 cut(s) 350, 462, 574
BstF5I GGATG 4 cut(s) 424, 460, 712, 749
BstFNI CGCG 3 cut(s) 158, 163, 229
BstH2I RGCGCY 1 cut(s) 95
BstHHI GCGC 5 cut(s) 94, 163, 165, 229, 308
BstKTI GATC 2 cut(s) 294, 661
BstMAI GTCTC 4 cut(s) 9, 452, 644, 699
BstMBI GATC 2 cut(s) 291, 658
BstMCI CGRYCG 1 cut(s) 294
BstMWI GCNNNNNNNGC 7 cut(s) 155, 160, 167, 226, 235, 308, 322
BstNI CCWGG 3 cut(s) 53, 89, 119
BstSCI CCNGG 4 cut(s) 51, 87, 117, 165
BstUI CGCG 3 cut(s) 158, 163, 229
BstV1I GCAGC 2 cut(s) 295, 600
BstXI CCANNNNNNTGG 1 cut(s) 88
BsuRI GGCC 2 cut(s) 170, 302
BtsCI GGATG 4 cut(s) 424, 460, 712, 749
BtsI GCAGTG 1 cut(s) 240
BtsIMutI CAGTG 2 cut(s) 240, 723
Cac8I GCNNGC 5 cut(s) 156, 163, 231, 300, 304
CfoI GCGC 5 cut(s) 94, 163, 165, 229, 308
Cfr13I GGNCC 3 cut(s) 168, 241, 300
CseI GACGC 3 cut(s) 65, 226, 238
Csp6I GTAC 1 cut(s) 477
CviQI GTAC 1 cut(s) 477
DdeI CTNAG 3 cut(s) 350, 462, 574
DinI GGCGCC 1 cut(s) 93
DpnI GATC 2 cut(s) 293, 660
DpnII GATC 2 cut(s) 291, 658
DrdI GACNNNNNNGTC 1 cut(s) 337
DseDI GACNNNNNNGTC 1 cut(s) 337
Eam1104I CTCTTC 2 cut(s) 134, 637
EarI CTCTTC 2 cut(s) 134, 637
EciI GGCGGA 1 cut(s) 350
Ecl136II GAGCTC 2 cut(s) 154, 280
Eco130I CCWWGG 1 cut(s) 244
Eco24I GRGCYC 3 cut(s) 156, 203, 282
Eco31I GGTCTC 2 cut(s) 644, 699
Eco47I GGWCC 1 cut(s) 241
Eco53kI GAGCTC 2 cut(s) 154, 280
Eco57I CTGAAG 1 cut(s) 534
EcoICRI GAGCTC 2 cut(s) 154, 280
EcoRII CCWGG 3 cut(s) 51, 87, 117
EcoT14I CCWWGG 1 cut(s) 244
EcoT38I GRGCYC 3 cut(s) 156, 203, 282
EgeI GGCGCC 1 cut(s) 93
EheI GGCGCC 1 cut(s) 93
ErhI CCWWGG 1 cut(s) 244
FaiI YATR 3 cut(s) 426, 468, 486
FauI CCCGC 2 cut(s) 222, 291
Fnu4HI GCNGC 5 cut(s) 60, 63, 159, 309, 614
FokI GGATG 3 cut(s) 431, 467, 699
FriOI GRGCYC 3 cut(s) 156, 203, 282
Fsp4HI GCNGC 5 cut(s) 60, 63, 159, 309, 614
FspBI CTAG 2 cut(s) 342, 734
GlaI GCGC 5 cut(s) 93, 162, 164, 228, 307
GluI GCNGC 5 cut(s) 60, 63, 159, 309, 614
GsaI CCCAGC 1 cut(s) 609
HaeII RGCGCY 1 cut(s) 95
HaeIII GGCC 2 cut(s) 170, 302
HapII CCGG 3 cut(s) 46, 166, 178
HgaI GACGC 3 cut(s) 65, 226, 238
HhaI GCGC 5 cut(s) 94, 163, 165, 229, 308
Hin1I GRCGYC 3 cut(s) 57, 92, 249
Hin6I GCGC 5 cut(s) 92, 161, 163, 227, 306
HinP1I GCGC 5 cut(s) 92, 161, 163, 227, 306
HinfI GANTC 5 cut(s) 124, 186, 338, 519, 694
HpaII CCGG 3 cut(s) 46, 166, 178
HphI GGTGA 2 cut(s) 70, 370
Hpy188I TCNGA 5 cut(s) 24, 193, 351, 577, 743
Hpy188III TCNNGA 2 cut(s) 406, 702
Hpy99I CGWCG 2 cut(s) 107, 140
HpyAV CCTTC 2 cut(s) 509, 541
HpyCH4V TGCA 1 cut(s) 593
HpyF10VI GCNNNNNNNGC 7 cut(s) 155, 160, 167, 226, 235, 308, 322
HpyF3I CTNAG 3 cut(s) 350, 462, 574
Hsp92I GRCGYC 3 cut(s) 57, 92, 249
HspAI GCGC 5 cut(s) 92, 161, 163, 227, 306
KasI GGCGCC 1 cut(s) 91
Kzo9I GATC 2 cut(s) 291, 658
LmnI GCTCC 5 cut(s) 48, 54, 146, 198, 277
Lsp1109I GCAGC 2 cut(s) 295, 600
LweI GCATC 1 cut(s) 734
MaeI CTAG 2 cut(s) 342, 734
MaeIII GTNAC 3 cut(s) 76, 214, 619
MalI GATC 2 cut(s) 293, 660
MboI GATC 2 cut(s) 291, 658
MboII GAAGA 5 cut(s) 121, 124, 624, 627, 696
MhlI GDGCHC 3 cut(s) 156, 203, 282
MluCI AATT 3 cut(s) 368, 443, 594
Mly113I GGCGCC 1 cut(s) 92
MlyI GAGTC 3 cut(s) 347, 528, 688
MmeI TCCRAC 2 cut(s) 216, 721
MroXI GAANNNNTTC 1 cut(s) 128
MseI TTAA 1 cut(s) 674
MspI CCGG 3 cut(s) 46, 166, 178
MspR9I CCNGG 4 cut(s) 53, 89, 119, 167
MvaI CCWGG 3 cut(s) 53, 89, 119
MvnI CGCG 3 cut(s) 158, 163, 229
MwoI GCNNNNNNNGC 7 cut(s) 155, 160, 167, 226, 235, 308, 322
NarI GGCGCC 1 cut(s) 92
NciI CCSGG 1 cut(s) 167
NdeII GATC 2 cut(s) 291, 658
NlaIV GGNNCC 4 cut(s) 50, 93, 142, 200
NmeAIII GCCGAG 3 cut(s) 175, 281, 337
NmuCI GTSAC 2 cut(s) 76, 214
PalAI GGCGCGCC 1 cut(s) 161
PauI GCGCGC 1 cut(s) 161
PdmI GAANNNNTTC 1 cut(s) 128
PfeI GAWTC 2 cut(s) 124, 186
PflMI CCANNNNNTGG 2 cut(s) 718, 722
PfoI TCCNGGA 1 cut(s) 51
PkrI GCNGC 5 cut(s) 61, 64, 160, 310, 615
Ple19I CGATCG 1 cut(s) 294
PleI GAGTC 3 cut(s) 346, 527, 688
PluTI GGCGCC 1 cut(s) 95
PpsI GAGTC 3 cut(s) 346, 527, 688
Psp124BI GAGCTC 2 cut(s) 156, 282
Psp6I CCWGG 3 cut(s) 51, 87, 117
PspFI CCCAGC 1 cut(s) 605
PspGI CCWGG 3 cut(s) 51, 87, 117
PspN4I GGNNCC 4 cut(s) 50, 93, 142, 200
PspPI GGNCC 3 cut(s) 168, 241, 300
PteI GCGCGC 1 cut(s) 161
PvuI CGATCG 1 cut(s) 294
RsaI GTAC 1 cut(s) 478
RsaNI GTAC 1 cut(s) 477
SacI GAGCTC 2 cut(s) 156, 282
SaqAI TTAA 1 cut(s) 674
SatI GCNGC 5 cut(s) 60, 63, 159, 309, 614
Sau3AI GATC 2 cut(s) 291, 658
Sau96I GGNCC 3 cut(s) 168, 241, 300
SchI GAGTC 3 cut(s) 347, 528, 688
ScrFI CCNGG 4 cut(s) 53, 89, 119, 167
SduI GDGCHC 3 cut(s) 156, 203, 282
SfaNI GCATC 1 cut(s) 734
SfoI GGCGCC 1 cut(s) 93
SgsI GGCGCGCC 1 cut(s) 161
SinI GGWCC 1 cut(s) 241
SmlI CTYRAG 2 cut(s) 221, 533
SmoI CTYRAG 2 cut(s) 221, 533
SpeI ACTAGT 1 cut(s) 733
Sse9I AATT 3 cut(s) 368, 443, 594
SsiI CCGC 9 cut(s) 60, 63, 70, 115, 158, 229, 298, 335, 709
SspDI GGCGCC 1 cut(s) 91
SspMI CTAG 2 cut(s) 342, 734
SstI GAGCTC 2 cut(s) 156, 282
StyD4I CCNGG 4 cut(s) 51, 87, 117, 165
StyI CCWWGG 1 cut(s) 244
TaqI TCGA 5 cut(s) 135, 282, 290, 601, 701
TasI AATT 3 cut(s) 368, 443, 594
TauI GCSGC 3 cut(s) 62, 65, 161
TfiI GAWTC 2 cut(s) 124, 186
Tru1I TTAA 1 cut(s) 674
Tru9I TTAA 1 cut(s) 674
TscAI CASTG 2 cut(s) 240, 723
TseFI GTSAC 2 cut(s) 76, 214
TseI GCWGC 2 cut(s) 308, 613
Tsp45I GTSAC 2 cut(s) 76, 214
TspRI CASTG 2 cut(s) 240, 723
Van91I CCANNNNNTGG 2 cut(s) 718, 722
VpaK11BI GGWCC 1 cut(s) 241
XapI RAATTY 1 cut(s) 368
XcmI CCANNNNNNNNNTGG 2 cut(s) 22, 719
XmnI GAANNNNTTC 1 cut(s) 128
XspI CTAG 2 cut(s) 342, 734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.