FvH4_7g07130

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0001590
Physical Location & Seq
Reverse (-)
7076152 .. 7087198
11047 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g07130.t5

Sequence Viewer

Length: 3381 bp
ATGACGAGCCATGCTGATAGTTCAGTGCAACACAAAAACACCACACCCATCCAACGCTGCATATCCCACCGCTTCTCCACAATAGCCTCCCAAAACCCTAACAAAATCGCAGTCATACACGCCGCCGCCGAGTCCCGCCGCACCCGCACCTCCGTCGCCGCCGATGCTTCTTCTTCCGTCCAAGCCGTCTACGGCGGCGACCGGTCCTTCAAGTACTCGGATTTAATCTCCGCCGTCGATTCCCTCACCTCGCAGCTCCGTCACTTTAAGCTTGATTCGCCTTCGTCAGAGCTCCGGCCGAGAATTTTCGGCGTATATATGGCGCCGTCGGTGGAGTACATAGTCTCGGCGCTCTCGGTTTTGCGGTGCGGCGAGGCTTTTCTTCCTCTGGATCCGTCCTGGCCAAAACAGCGGCTGCTCTCCGTCGTTGCTTCCGCCGATGTCGACCTGATTATCACCTCCACGACGCCGTTTGGGTACGAGTTGGAGTCCAGTTGGCTAGAGAAGACTTCCAATCGGAGGTTTCTGTGGTTTTCCATGGGCGGTGGAGAGTCTGTGACAGTGACTGATGGAGGAGAGTATGAGAAAGAGAAAGAGAAAGAGAGGCTGTTCTGTTATGTGATGTACACTTCGGGCTCGACGGGGAAGCCTAAAGGTGTGTGTGGAACTGAACAAGGTCTGTGGAATCGGTTTCGGTGGATGGAAGAGTTGTATCCATTGAAAGGAGAGGAGGTCTTGTTGTTCAAGACTGCAGTGAGCTTTGTTGATCATTTGCAAGAGTTTCTCAGTGCTATTCTTACTGGTTGTACTTTGGTCATACCTCCTTTCAATCAGCTTAAACAGAATGTGTTCTCCATTCTCGATTTTCTACAGGCTTATTCTATCAGTAGACTTACTGCTGTTCCCTCACTGATGAGAGTAATCCTTCCAGCTTTGCAAGGTCGAGACTATCAGCAGCTTCTAAGTTCACTGAATTTGTTAGTGCTTAGCGGAGAGGTTTTGCCTTTATCCTTGTGGGATATGCTGTCAAAGAGATTACCAAGGACCTCTATTTTGAATTTGTATGGGAGTACAGAGGTGTCAGGTGATTGCACATATTTTGACTGCAAGAGGTTGCCAATGATTTTGGAGACAGAGACACTAACAAGTGTTCCAATTGGTATACCTATAGCTGGTTGTGATGTTGTGATTGTTAGTGATGATGATGTACTTGATGAAGGAGAAATTTTTGTTGCTGGTCTCTGCAATTCTTCTGGATACTATTCTGATTCTACATTTACTCCTCTGGACACTGTCAAATTACCTCCTAGTTCTCTTTGTGACAGTTCTGTAAATGGACATGAAAGTCGATCATATTTTCAAACTGGTGATTTTGCCAAACAACTGCATAGTGGTGACTTGGTTTTTTTGGGAAGAAAGGACCGCACTATTAAGCACAATGGGCAGCGTATTGCTTTAGAGGAAATTGAACATATATTGGTGGGACATCCAGATGTAACTGATGCTGCTGTTGTTTTTCATCATGGTCAAGGGGAACTAATGCAACTTGTAGCATTTATAATATTAAAGGAGGGACAATCTGATGAAACATTCAGATCTTCCATCAAAAGTTGGATGGTTGACAAACTTCCACTGGCAATGATTCCTGGCCGCATTGTTATAATGAAATCATTTCCTGTATCTACTAGTGGGAAAGTTGATTACACTTTGTTAGCAGATTCAGTATTTCGAGCAAAACATATCCAATATGAATTTGATCAAACTGGGAGAAGCAATTTACTGCAAGTAATTAAAAAGGCCTTCAATCATGTTTTAATGGCTGAAGAGGTTTCCGATGATGATGATTTCTTTATGATTGGTGGAAATTCTATTGCTGCTGCACATCTTGCTAACAATATAGGAGTTGATATGAGATTGATATATAGCTTTCCAACTCCATCTAAGCTTTGTATGGCTCTTCTAGAGAGAAAAGGACCATTCAGTATGAAAGTTAAAGTGGACTCTGAATCTGTGATAAATCAGGGTGGGGGAAAGAACATGTTTCCTGTCTATGACACTCCTACTGATGTTAATTTTGAACAGTTGAAAGCTCAGTCCAAACGGTTGAAAGTAAATTCAAGTATAACTTTGCAGAGTGCTAGTCCAGCTGATGGGTATCCATGGAGTTCTGTGCAAAACTTCATGTCATGTTCTTTCACCAGGTGCAACAAGGTTGTGTATGAGAGTTTTAGGGTGAATGAAGTTTATGAAGCAGCCGGTTCTGTGGTTGTCTCAAAAAATAGAAAAGTTCTTTTGGAAGAATTATGGAAAGTTGACATGGGGTCCTGTGTTGATGCATCACCGCTTATTGTTTGTAAAGACCAGGATATCTACTTATTCATTGGATCTCACTCGCAAAAGTTTGCGTGTGTTAATGCCAGAAGTGGTTCCATCCAGTGGGAGTTACAACTCGAAGGACGTGTAGAATGTTCAGCAGCAATTCTTGGCAACTTTACCCAGGTTGTAGTTGGATGCTACAAAGGGAAGATATACTTCCTGGATTCTTCGAATGGTAACATATGTTGGACTTTCCAAACGTCCGGTGAGGTAAAGTCACAGCCAGTTATAGACAGTCAAAGACAATTGATCTGGTGTGGGTCATATGACCATAATTTGTATGCGCTAGATTATAAAAATCATTGCTGTGTTTATCAGCTTCCATGTGGCGGGAGTATCTGTGGGTCTCCTGCAATAGATGAGATGAATAATATACTTTATGTGGCTACTACTGGAGGCCAAATGACAGCCATTTCAATAATGGCATTGCCATTTTCTATCATGTGGGTGCATGAGTTAGAAGTGCCAGTATTTGGATCTCTTGCCATCAATTCTCTGAATGGAAATATTATATGTTGCTTGGTGGATGGGCATGTTCTTGCATTTAACACAAGTGGATCTATTATTTGGAGGTTTAGAACTGCTGGGCCTGTATTTGCTGGAGCGTGCATATCTTCCGGACTTCCCTTCCAGGTACTTGTTTGTTCAAGAGATGGAAGCATCTATTCACTTAAACTTGAAACGGGAGGTTTGCTCTGGGAATACAATGTTAAGGATCCAATTACTTCATCTGCCTATGTTGATGAGCACCTGAGCTTGGTGTCTGATCAGTCTAAATTTCCGGACAGCAGGATGGTTTGTGTATGTTCCAGCTCCGGAAGCATAATTGTAATTCAGGTTAACTTGGATGCCACCAAAGGTGCAAGTGCAAAGAATTTCGATGTTGAGGAATTTGCAAGGTTGGAACTCACAGGAGATGTATTCTCTTCCCCTGTAATGATTGGTGGCAGAATTTTCGTTGGTTGTAGGGATGATTATGTGCGCTGCATTGCTTTTAAGCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C PP-binding PQQ PQQ_2 PQQ_3

Protein Analysis

1127

Amino Acids

124.19

Weight (kDa)

5.64

Isoelectric Point (pI)

41.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 60 - 421 1.6e-49 AMP-binding enzyme
AMP-binding_C PF13193 488 - 565 1.1e-10 AMP-binding enzyme C-terminal domain
Beta-prop_ACSF4 PF13570 773 - 811 3.2e-09 Beta-alanine-activating enzyme, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1559, 1661, 2670
AasI GACNNNNNNGTC 1 cut(s) 1344
AccB1I GGYRCC 1 cut(s) 322
AccB7I CCANNNNNTGG 3 cut(s) 2150, 2436, 2849
AccI GTMKAC 4 cut(s) 189, 444, 889, 1162
AccIII TCCGGA 3 cut(s) 2993, 3157, 3191
AclWI GGATC 7 cut(s) 386, 399, 2392, 2860, 2941, 3086, 3099
AcoI YGGCCR 3 cut(s) 296, 401, 1648
AcuI CTGAAG 1 cut(s) 1842
AcyI GRCGYC 2 cut(s) 323, 467
AdeI CACNNNGTG 1 cut(s) 2202
AfaI GTAC 8 cut(s) 215, 338, 479, 626, 808, 1072, 1209, 3012
AfiI CCNNNNNNNGG 8 cut(s) 519, 722, 1172, 2150, 2436, 2705, 2849, 3133
AflIII ACRYGT 2 cut(s) 2037, 2458
AgeI ACCGGT 1 cut(s) 201
AhdI GACNNNNNGTC 1 cut(s) 2320
AhlI ACTAGT 1 cut(s) 1685
AjiI CACGTC 1 cut(s) 2459
AjnI CCWGG 7 cut(s) 398, 1645, 2198, 2361, 2496, 2535, 3006
Alw21I GWGCWC 2 cut(s) 294, 3126
Alw26I GTCTC 7 cut(s) 349, 939, 1124, 1130, 1244, 2275, 2727
AlwI GGATC 7 cut(s) 386, 399, 2392, 2860, 2941, 3086, 3099
AlwNI CAGNNNCTG 2 cut(s) 415, 566
Aor13HI TCCGGA 3 cut(s) 2993, 3157, 3191
AoxI GGCC 6 cut(s) 296, 401, 1648, 1797, 2773, 2963
ArsI GACNNNNNNTTYG 4 cut(s) 96, 128, 1036, 1068
AsiGI ACCGGT 1 cut(s) 201
Asp700I GAANNNNTTC 2 cut(s) 848, 2425
AspLEI GCGC 4 cut(s) 325, 352, 2662, 3360
AspS9I GGNCC 6 cut(s) 204, 1044, 1420, 1973, 2322, 2963
AsuHPI GGTGA 9 cut(s) 238, 448, 1097, 1379, 1406, 2188, 2245, 2331, 2594
AsuII TTCGAA 1 cut(s) 2546
AvaII GGWCC 5 cut(s) 204, 1044, 1420, 1973, 2322
BalI TGGCCA 1 cut(s) 403
BamHI GGATCC 2 cut(s) 391, 3091
BanI GGYRCC 1 cut(s) 322
BanII GRGCYC 2 cut(s) 294, 638
BbsI GAAGAC 1 cut(s) 512
Bbv12I GWGCWC 2 cut(s) 294, 3126
BceAI ACGGC 5 cut(s) 170, 208, 218, 310, 454
BcgI CGANNNNNNTGC 4 cut(s) 136, 170, 3313, 3347
BciT130I CCWGG 7 cut(s) 400, 1647, 2200, 2363, 2498, 2537, 3008
BciVI GTATCC 3 cut(s) 723, 1250, 2166
BclI TGATCA 3 cut(s) 766, 1756, 3142
BcoDI GTCTC 7 cut(s) 349, 939, 1124, 1130, 1244, 2275, 2727
BcuI ACTAGT 1 cut(s) 1685
BfaI CTAG 7 cut(s) 500, 1308, 1686, 1961, 2139, 2663, 3379
BfmI CTRYAG 3 cut(s) 750, 869, 1167
BfoI RGCGCY 2 cut(s) 326, 353
BfuI GTATCC 3 cut(s) 723, 1250, 2166
BglII AGATCT 1 cut(s) 1595
BlpI GCTNAGC 1 cut(s) 986
BmcAI AGTACT 1 cut(s) 215
Bme1390I CCNGG 7 cut(s) 400, 1647, 2200, 2363, 2498, 2537, 3008
Bme18I GGWCC 5 cut(s) 204, 1044, 1420, 1973, 2322
BmeRI GACNNNNNGTC 1 cut(s) 2320
BmgBI CACGTC 1 cut(s) 2459
BmgT120I GGNCC 6 cut(s) 204, 1044, 1420, 1973, 2322, 2963
BmiI GGNNCC 5 cut(s) 324, 393, 2323, 2428, 3093
BmrFI CCNGG 7 cut(s) 400, 1647, 2200, 2363, 2498, 2537, 3008
BmrI ACTGGG 1 cut(s) 1773
BmsI GCATC 7 cut(s) 154, 1492, 2323, 2345, 2501, 3045, 3214
BmuI ACTGGG 1 cut(s) 1773
BpiI GAAGAC 1 cut(s) 512
BplI GAGNNNNNCTC 2 cut(s) 3054, 3086
BpmI CTGGAG 2 cut(s) 2790, 2997
Bpu10I CCTNAGC 1 cut(s) 3128
Bpu1102I GCTNAGC 1 cut(s) 986
Bpu14I TTCGAA 1 cut(s) 2546
BsaBI GATNNNNATC 1 cut(s) 2154
BsaHI GRCGYC 2 cut(s) 323, 467
BsaI GGTCTC 2 cut(s) 1244, 2727
BsaJI CCNNGG 4 cut(s) 537, 1040, 2159, 2496
BsaWI WCCGGW 5 cut(s) 201, 2579, 2993, 3157, 3191
Bsc4I CCNNNNNNNGG 8 cut(s) 519, 722, 1172, 2150, 2436, 2705, 2849, 3133
Bse118I RCCGGY 2 cut(s) 201, 2255
Bse1I ACTGG 9 cut(s) 492, 805, 1369, 1638, 1768, 2434, 2600, 2773, 2843
Bse3DI GCAATG 4 cut(s) 1644, 2677, 2801, 3363
Bse8I GATNNNNATC 1 cut(s) 2154
BseAI TCCGGA 3 cut(s) 2993, 3157, 3191
BseBI CCWGG 7 cut(s) 400, 1647, 2200, 2363, 2498, 2537, 3008
BseDI CCNNGG 4 cut(s) 537, 1040, 2159, 2496
BseJI GATNNNNATC 1 cut(s) 2154
BseLI CCNNNNNNNGG 8 cut(s) 519, 722, 1172, 2150, 2436, 2705, 2849, 3133
BseMI GCAATG 4 cut(s) 1644, 2677, 2801, 3363
BseMII CTCAG 3 cut(s) 799, 2105, 3119
BseNI ACTGG 9 cut(s) 492, 805, 1369, 1638, 1768, 2434, 2600, 2773, 2843
BseRI GAGGAG 3 cut(s) 588, 743, 1272
BseX3I CGGCCG 1 cut(s) 296
BseYI CCCAGC 1 cut(s) 2960
BsgI GTGCAG 1 cut(s) 1863
Bsh1285I CGRYCG 2 cut(s) 202, 299
BshFI GGCC 6 cut(s) 298, 403, 1650, 1799, 2775, 2965
BshNI GGYRCC 1 cut(s) 322
BshTI ACCGGT 1 cut(s) 201
BsiEI CGRYCG 2 cut(s) 202, 299
BsiHKAI GWGCWC 2 cut(s) 294, 3126
BsiSI CCGG 7 cut(s) 202, 295, 2256, 2580, 2994, 3158, 3192
BslFI GGGAC 3 cut(s) 118, 1497, 1587
BslI CCNNNNNNNGG 8 cut(s) 519, 722, 1172, 2150, 2436, 2705, 2849, 3133
BsmAI GTCTC 7 cut(s) 349, 939, 1124, 1130, 1244, 2275, 2727
BsmFI GGGAC 3 cut(s) 118, 1497, 1587
BsnI GGCC 6 cut(s) 298, 403, 1650, 1799, 2775, 2965
Bso31I GGTCTC 2 cut(s) 1244, 2727
Bsp119I TTCGAA 1 cut(s) 2546
Bsp1286I GDGCHC 3 cut(s) 294, 638, 3126
Bsp13I TCCGGA 3 cut(s) 2993, 3157, 3191
Bsp1407I TGTACA 1 cut(s) 624
Bsp1720I GCTNAGC 1 cut(s) 986
Bsp19I CCATGG 2 cut(s) 537, 2159
BspANI GGCC 6 cut(s) 298, 403, 1650, 1799, 2775, 2965
BspCNI CTCAG 3 cut(s) 798, 2104, 3120
BspEI TCCGGA 3 cut(s) 2993, 3157, 3191
BspLI GGNNCC 5 cut(s) 324, 393, 2323, 2428, 3093
BspMAI CTGCAG 1 cut(s) 754
BspPI GGATC 7 cut(s) 386, 399, 2392, 2860, 2941, 3086, 3099
BspQI GCTCTTC 1 cut(s) 1962
BspT104I TTCGAA 1 cut(s) 2546
BspT107I GGYRCC 1 cut(s) 322
BspTNI GGTCTC 2 cut(s) 1244, 2727
BsrDI GCAATG 4 cut(s) 1644, 2677, 2801, 3363
BsrFI RCCGGY 2 cut(s) 201, 2255
BsrGI TGTACA 1 cut(s) 624
BsrI ACTGG 9 cut(s) 492, 805, 1369, 1638, 1768, 2434, 2600, 2773, 2843
BssAI RCCGGY 2 cut(s) 201, 2255
BssECI CCNNGG 4 cut(s) 537, 1040, 2159, 2496
BssNAI GTATAC 1 cut(s) 1163
BssNI GRCGYC 2 cut(s) 323, 467
BssT1I CCWWGG 3 cut(s) 537, 1040, 2159
Bst1107I GTATAC 1 cut(s) 1163
Bst2UI CCWGG 7 cut(s) 400, 1647, 2200, 2363, 2498, 2537, 3008
Bst4CI ACNGT 6 cut(s) 562, 1294, 1325, 2082, 2103, 2612
Bst6I CTCTTC 4 cut(s) 699, 1818, 1962, 3307
BstACI GRCGYC 2 cut(s) 323, 467
BstAPI GCANNNNNTGC 1 cut(s) 1886
BstAUI TGTACA 1 cut(s) 624
BstBI TTCGAA 1 cut(s) 2546
BstC8I GCNNGC 1 cut(s) 2983
BstDEI CTNAG 6 cut(s) 785, 962, 986, 1941, 2091, 3128
BstDSI CCRYGG 2 cut(s) 537, 2159
BstH2I RGCGCY 2 cut(s) 326, 353
BstHHI GCGC 4 cut(s) 325, 352, 2662, 3360
BstMAI GTCTC 7 cut(s) 349, 939, 1124, 1130, 1244, 2275, 2727
BstMCI CGRYCG 2 cut(s) 202, 299
BstMWI GCNNNNNNNGC 8 cut(s) 144, 164, 277, 409, 1441, 1886, 2144, 3195
BstNI CCWGG 7 cut(s) 400, 1647, 2200, 2363, 2498, 2537, 3008
BstNSI RCATGY 2 cut(s) 2041, 2912
BstSCI CCNGG 7 cut(s) 398, 1645, 2198, 2361, 2496, 2535, 3006
BstSFI CTRYAG 3 cut(s) 750, 869, 1167
BstV2I GAAGAC 1 cut(s) 512
BstX2I RGATCY 6 cut(s) 391, 1595, 2384, 2852, 2933, 3091
BstYI RGATCY 6 cut(s) 391, 1595, 2384, 2852, 2933, 3091
BstZ17I GTATAC 1 cut(s) 1163
BstZI CGGCCG 1 cut(s) 296
BsuI GTATCC 3 cut(s) 723, 1250, 2166
BsuRI GGCC 6 cut(s) 298, 403, 1650, 1799, 2775, 2965
BtgI CCRYGG 2 cut(s) 537, 2159
BtrI CACGTC 1 cut(s) 2459
BtsI GCAGTG 1 cut(s) 759
BtsIMutI CAGTG 9 cut(s) 30, 567, 759, 793, 908, 968, 1290, 1631, 2441
Cac8I GCNNGC 1 cut(s) 2983
CaiI CAGNNNCTG 2 cut(s) 415, 566
CfoI GCGC 4 cut(s) 325, 352, 2662, 3360
Cfr10I RCCGGY 2 cut(s) 201, 2255
Cfr13I GGNCC 6 cut(s) 204, 1044, 1420, 1973, 2322, 2963
CseI GACGC 1 cut(s) 475
CsiI ACCWGGT 1 cut(s) 2198
Csp6I GTAC 8 cut(s) 214, 337, 478, 625, 807, 1071, 1208, 3011
CspAI ACCGGT 1 cut(s) 201
CspCI CAANNNNNGTGG 2 cut(s) 662, 697
CviQI GTAC 8 cut(s) 214, 337, 478, 625, 807, 1071, 1208, 3011
DdeI CTNAG 6 cut(s) 785, 962, 986, 1941, 2091, 3128
DinI GGCGCC 1 cut(s) 324
DraIII CACNNNGTG 1 cut(s) 2202
DrdI GACNNNNNNGTC 1 cut(s) 1344
DriI GACNNNNNGTC 1 cut(s) 2320
DseDI GACNNNNNNGTC 1 cut(s) 1344
EaeI YGGCCR 3 cut(s) 296, 401, 1648
EagI CGGCCG 1 cut(s) 296
Eam1104I CTCTTC 4 cut(s) 699, 1818, 1962, 3307
Eam1105I GACNNNNNGTC 1 cut(s) 2320
EarI CTCTTC 4 cut(s) 699, 1818, 1962, 3307
EciI GGCGGA 2 cut(s) 220, 424
Ecl136II GAGCTC 1 cut(s) 292
EclXI CGGCCG 1 cut(s) 296
Eco130I CCWWGG 3 cut(s) 537, 1040, 2159
Eco147I AGGCCT 1 cut(s) 1799
Eco24I GRGCYC 2 cut(s) 294, 638
Eco31I GGTCTC 2 cut(s) 1244, 2727
Eco32I GATATC 1 cut(s) 2368
Eco47I GGWCC 5 cut(s) 204, 1044, 1420, 1973, 2322
Eco52I CGGCCG 1 cut(s) 296
Eco53kI GAGCTC 1 cut(s) 292
Eco57I CTGAAG 1 cut(s) 1842
EcoICRI GAGCTC 1 cut(s) 292
EcoO109I RGGNCCY 2 cut(s) 1044, 2322
EcoRII CCWGG 7 cut(s) 398, 1645, 2198, 2361, 2496, 2535, 3006
EcoRV GATATC 1 cut(s) 2368
EcoT14I CCWWGG 3 cut(s) 537, 1040, 2159
EcoT22I ATGCAT 1 cut(s) 2338
EcoT38I GRGCYC 2 cut(s) 294, 638
EgeI GGCGCC 1 cut(s) 324
EheI GGCGCC 1 cut(s) 324
ErhI CCWWGG 3 cut(s) 537, 1040, 2159
FalI AAGNNNNNCTT 4 cut(s) 2110, 2142, 2516, 2548
FaqI GGGAC 3 cut(s) 118, 1497, 1587
FauI CCCGC 3 cut(s) 143, 152, 2699
FauNDI CATATG 2 cut(s) 2558, 2641
FbaI TGATCA 3 cut(s) 766, 1756, 3142
FblI GTMKAC 4 cut(s) 189, 444, 889, 1162
FriOI GRGCYC 2 cut(s) 294, 638
FspBI CTAG 7 cut(s) 500, 1308, 1686, 1961, 2139, 2663, 3379
GlaI GCGC 4 cut(s) 324, 351, 2661, 3359
GsaI CCCAGC 1 cut(s) 2964
GsuI CTGGAG 2 cut(s) 2790, 2997
HaeII RGCGCY 2 cut(s) 326, 353
HaeIII GGCC 6 cut(s) 298, 403, 1650, 1799, 2775, 2965
HapII CCGG 7 cut(s) 202, 295, 2256, 2580, 2994, 3158, 3192
HgaI GACGC 1 cut(s) 475
HhaI GCGC 4 cut(s) 325, 352, 2662, 3360
Hin1I GRCGYC 2 cut(s) 323, 467
Hin6I GCGC 4 cut(s) 323, 350, 2660, 3358
HinP1I GCGC 4 cut(s) 323, 350, 2660, 3358
HincII GTYRAC 4 cut(s) 445, 1621, 2314, 3217
HindII GTYRAC 4 cut(s) 445, 1621, 2314, 3217
HindIII AAGCTT 2 cut(s) 269, 1943
HpaI GTTAAC 1 cut(s) 3217
HpaII CCGG 7 cut(s) 202, 295, 2256, 2580, 2994, 3158, 3192
HphI GGTGA 9 cut(s) 238, 448, 1097, 1379, 1406, 2188, 2245, 2331, 2594
Hpy99I CGWCG 6 cut(s) 158, 239, 331, 428, 469, 643
HpyAV CCTTC 7 cut(s) 217, 291, 935, 1211, 1810, 2447, 3013
HpyCH4III ACNGT 6 cut(s) 562, 1294, 1325, 2082, 2103, 2612
HpyCH4IV ACGT 2 cut(s) 2458, 2576
HpyF10VI GCNNNNNNNGC 8 cut(s) 144, 164, 277, 409, 1441, 1886, 2144, 3195
HpyF3I CTNAG 6 cut(s) 785, 962, 986, 1941, 2091, 3128
HpySE526I ACGT 2 cut(s) 2458, 2576
Hsp92I GRCGYC 2 cut(s) 323, 467
HspAI GCGC 4 cut(s) 323, 350, 2660, 3358
KasI GGCGCC 1 cut(s) 322
Kpn2I TCCGGA 3 cut(s) 2993, 3157, 3191
Ksp22I TGATCA 3 cut(s) 766, 1756, 3142
KspAI GTTAAC 1 cut(s) 3217
LguI GCTCTTC 1 cut(s) 1962
LmnI GCTCC 4 cut(s) 261, 297, 2978, 3194
LweI GCATC 7 cut(s) 154, 1492, 2323, 2345, 2501, 3045, 3214
MabI ACCWGGT 1 cut(s) 2198
MaeI CTAG 7 cut(s) 500, 1308, 1686, 1961, 2139, 2663, 3379
MaeII ACGT 2 cut(s) 2458, 2576
MaeIII GTNAC 9 cut(s) 260, 556, 562, 1319, 1394, 1495, 2442, 2552, 2592
MfeI CAATTG 2 cut(s) 1155, 2621
MflI RGATCY 6 cut(s) 391, 1595, 2384, 2852, 2933, 3091
MhlI GDGCHC 3 cut(s) 294, 638, 3126
MlsI TGGCCA 1 cut(s) 403
MluNI TGGCCA 1 cut(s) 403
Mly113I GGCGCC 1 cut(s) 323
MlyI GAGTC 4 cut(s) 140, 497, 560, 1994
MmeI TCCRAC 7 cut(s) 76, 465, 1592, 1955, 2488, 2543, 3258
Mox20I TGGCCA 1 cut(s) 403
Mph1103I ATGCAT 1 cut(s) 2338
MroI TCCGGA 3 cut(s) 2993, 3157, 3191
MroXI GAANNNNTTC 2 cut(s) 848, 2425
MscI TGGCCA 1 cut(s) 403
MslI CAYNNNNRTG 4 cut(s) 1392, 1491, 2682, 2822
Msp20I TGGCCA 1 cut(s) 403
MspA1I CMGCKG 2 cut(s) 412, 2147
MspI CCGG 7 cut(s) 202, 295, 2256, 2580, 2994, 3158, 3192
MspR9I CCNGG 7 cut(s) 400, 1647, 2200, 2363, 2498, 2537, 3008
MunI CAATTG 2 cut(s) 1155, 2621
MvaI CCWGG 7 cut(s) 400, 1647, 2200, 2363, 2498, 2537, 3008
MwoI GCNNNNNNNGC 8 cut(s) 144, 164, 277, 409, 1441, 1886, 2144, 3195
NarI GGCGCC 1 cut(s) 323
NcoI CCATGG 2 cut(s) 537, 2159
NdeI CATATG 2 cut(s) 2558, 2641
NlaIV GGNNCC 5 cut(s) 324, 393, 2323, 2428, 3093
NmeAIII GCCGAG 3 cut(s) 154, 324, 326
NmuCI GTSAC 6 cut(s) 260, 556, 562, 1319, 1394, 2592
NsiI ATGCAT 1 cut(s) 2338
NspI RCATGY 2 cut(s) 2041, 2912
NspV TTCGAA 1 cut(s) 2546
PceI AGGCCT 1 cut(s) 1799
PciI ACATGT 1 cut(s) 2037
PciSI GCTCTTC 1 cut(s) 1962
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PdmI GAANNNNTTC 2 cut(s) 848, 2425
PfeI GAWTC 8 cut(s) 239, 275, 685, 1268, 1642, 1718, 2006, 2540
PflFI GACNNNGTC 1 cut(s) 1292
PflMI CCANNNNNTGG 3 cut(s) 2150, 2436, 2849
PfoI TCCNGGA 1 cut(s) 2535
PinAI ACCGGT 1 cut(s) 201
PleI GAGTC 4 cut(s) 139, 496, 559, 1994
PluTI GGCGCC 1 cut(s) 326
PpsI GAGTC 4 cut(s) 139, 496, 559, 1994
PpuMI RGGWCCY 2 cut(s) 1044, 2322
PscI ACATGT 1 cut(s) 2037
PsiI TTATAA 3 cut(s) 1559, 1661, 2670
Psp124BI GAGCTC 1 cut(s) 294
Psp5II RGGWCCY 2 cut(s) 1044, 2322
Psp6I CCWGG 7 cut(s) 398, 1645, 2198, 2361, 2496, 2535, 3006
PspFI CCCAGC 1 cut(s) 2960
PspGI CCWGG 7 cut(s) 398, 1645, 2198, 2361, 2496, 2535, 3006
PspN4I GGNNCC 5 cut(s) 324, 393, 2323, 2428, 3093
PspPI GGNCC 6 cut(s) 204, 1044, 1420, 1973, 2322, 2963
PspPPI RGGWCCY 2 cut(s) 1044, 2322
PstI CTGCAG 1 cut(s) 754
PstNI CAGNNNCTG 2 cut(s) 415, 566
PsuI RGATCY 6 cut(s) 391, 1595, 2384, 2852, 2933, 3091
PsyI GACNNNGTC 1 cut(s) 1292
PvuII CAGCTG 1 cut(s) 2147
RsaI GTAC 8 cut(s) 215, 338, 479, 626, 808, 1072, 1209, 3012
RsaNI GTAC 8 cut(s) 214, 337, 478, 625, 807, 1071, 1208, 3011
RseI CAYNNNNRTG 4 cut(s) 1392, 1491, 2682, 2822
SacI GAGCTC 1 cut(s) 294
SalI GTCGAC 1 cut(s) 443
SapI GCTCTTC 1 cut(s) 1962
Sau96I GGNCC 6 cut(s) 204, 1044, 1420, 1973, 2322, 2963
ScaI AGTACT 1 cut(s) 215
SchI GAGTC 4 cut(s) 140, 497, 560, 1994
ScrFI CCNGG 7 cut(s) 400, 1647, 2200, 2363, 2498, 2537, 3008
SduI GDGCHC 3 cut(s) 294, 638, 3126
SexAI ACCWGGT 1 cut(s) 2198
SfaNI GCATC 7 cut(s) 154, 1492, 2323, 2345, 2501, 3045, 3214
SfcI CTRYAG 3 cut(s) 750, 869, 1167
SfoI GGCGCC 1 cut(s) 324
SfuI TTCGAA 1 cut(s) 2546
SinI GGWCC 5 cut(s) 204, 1044, 1420, 1973, 2322
SmiMI CAYNNNNRTG 4 cut(s) 1392, 1491, 2682, 2822
SpeI ACTAGT 1 cut(s) 1685
SseBI AGGCCT 1 cut(s) 1799
SspDI GGCGCC 1 cut(s) 322
SspI AATATT 2 cut(s) 1563, 2884
SspMI CTAG 7 cut(s) 500, 1308, 1686, 1961, 2139, 2663, 3379
SstI GAGCTC 1 cut(s) 294
StuI AGGCCT 1 cut(s) 1799
StyD4I CCNGG 7 cut(s) 398, 1645, 2198, 2361, 2496, 2535, 3006
StyI CCWWGG 3 cut(s) 537, 1040, 2159
TaaI ACNGT 6 cut(s) 562, 1294, 1325, 2082, 2103, 2612
TaiI ACGT 2 cut(s) 2461, 2579
TatI WGTACW 6 cut(s) 213, 336, 624, 806, 1070, 1207
TauI GCSGC 8 cut(s) 125, 128, 141, 161, 198, 372, 415, 1653
TfiI GAWTC 8 cut(s) 239, 275, 685, 1268, 1642, 1718, 2006, 2540
TscAI CASTG 9 cut(s) 30, 567, 759, 793, 915, 975, 1297, 1638, 2441
TseFI GTSAC 6 cut(s) 260, 556, 562, 1319, 1394, 2592
Tsp45I GTSAC 6 cut(s) 260, 556, 562, 1319, 1394, 2592
TspGWI ACGGA 5 cut(s) 142, 166, 248, 384, 412
TspRI CASTG 9 cut(s) 30, 567, 759, 793, 915, 975, 1297, 1638, 2441
Tth111I GACNNNGTC 1 cut(s) 1292
Van91I CCANNNNNTGG 3 cut(s) 2150, 2436, 2849
VpaK11BI GGWCC 5 cut(s) 204, 1044, 1420, 1973, 2322
XbaI TCTAGA 1 cut(s) 1960
XceI RCATGY 2 cut(s) 2041, 2912
XcmI CCANNNNNNNNNTGG 2 cut(s) 2504, 2794
XmiI GTMKAC 4 cut(s) 189, 444, 889, 1162
XmnI GAANNNNTTC 2 cut(s) 848, 2425
XspI CTAG 7 cut(s) 500, 1308, 1686, 1961, 2139, 2663, 3379
ZrmI AGTACT 1 cut(s) 215
Zsp2I ATGCAT 1 cut(s) 2338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.