Prupe.2G098200_v2.0.a1

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0001590
Physical Location & Seq
Reverse (-)
15336978 .. 15348376
11399 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G098200.3

Sequence Viewer

Length: 3465 bp
ATGACGAGAAATGATGCACTAAATCTGAAACCCCAAATGATAAGCGCACATAAAAACACCCATTGCTGCATTTCCCGCGAGTTCTCCGCAGCAGCGGCCAACAACGCCAACAAAATCGCCGTTGTGCATGCCTCCGGTAGGGCCCTCCTTTCCCCACAACTCCGTTCCCGCACCTCCATCACAGAGGATGCTGCTCCTGCTCTCGTCCGGCAGCTCCTCTATGACGGCGACCACTCCTTCACCTACTCCCATCTCATCTCAGCCGTCGGTTCACTCACCTCCCAGCTCCTCTCCACCCCTCGACTTCCCTGTGATTACAACAAGCCCAGCCGGAGAATCTTCGGCTTATACATGCCGCCCTCGGCGGAGTACATAGTGTCCGTGCTTTCAGTTTTGAGGTGCGGAGAGGCCTTTCTGCCTCTGGACCCTTCGTGGCCCAAACAGAGGCTACTTTCAGTAATTTCCTCGGCCAATGTCGACCTCATCATCGCCTCCAGGACGCCGTTCGGGTTCGAATCAGACTCGAATTGGCTACCCGAGGCCTGCGGTGGTCGCGTTTTGTGGTTCTCAATGGAAGAGCGTAAAAATGGAGGGATCGATTGGGATTGGGCGTGCAAATGTGAGAGTGTGAAGGAGAGGCCGTGCTGGTGTTATTTGATGTACACTTCGGGTTCGACTGGGAAGCCGAAAGGTGTGTGTGGAACTGAGCAAGGTCTTTTGAATCGGTTTCTATGGATGCAAGAGTTGTATCCACTGTTTGGAGACGAGGTTTTGTTGTTCAAGACAGGGATAAGCTTTGTTGATCACCTGCAAGAGTTTCTTAGCGCTATTCTCACTGGTTGTACTTTGGTTATACCTCCTTTCAATCACCTTAAGCGCAATGTGTTTTCGCTTGTCGATTTTCTACAGGCTTACTTTGTCAATAGACTTACTGCTGTTCCATCACTTATGAGAGCAATCCTTCCTTCCTTACAAGGTCGAGATGATGAGCAGCTTCCAAGGTCATTGGATTTGTTAGTGTTAAGCGGCGAAGTTTTGCCTTTATCCTTATGGGATAAGCTCTCCAAGATGTTACCGAGGACCGCTATTTTGAATTTGTATGGGAGTACTGAGGTGTCTGGTGATTGTACATATTTTGATTGCAAGAGATTGCCATTGATTTTGGCGGCAGACCGACTAACAACTGTTCCAATTGGTATGCCTATTTTTAATTGTGATGTCGTACTTATTGGTGGTGATGATGTATCCAATGAGGGAGAAATTTATGTCGCTGGTGTATGCAATTCTTCTGGATACTATTCTGATTCTATGGTTACTCCTTTGGACACTGTAAGGTTGTCTCAAGATTCTGTTTGTTGCAGTTCTGTAAATGGACATAAAAGTCAATATTATTTTCGGACGGGTGATTTTGCTAAACAGCTTCGTAGCGGTGACTTGGTTTTCTTAGGTAGAAAAGACCGTACAATTAAGCTCAATGGGCAGCGTATTGCTTTAGAGGAAATTGAAGATACAGTGAGGGGACACTCAGAGGTAATTGATGCTGCTGTAATTTTTCATAAAGTTCAAGGGGAACTCATGCTACTCGTGGCATTTATAATATTAAGAGAGGGAATACCCAAGGAAATATTCGGAGCTACCATCAAAAGTTGGATGGCTGACAAACTTCCATTAGCAATGATTCCTGGCCACATTGTTGTTACTGAATCATTTCCAGTATCTTCTAGTGGCAAAATTAACTATGAGTTGTTGGCAGACTCAGTATTTCTTGCAAAGCATGTCGAAGATGGGCTTGGTGACGTTGGGAGTAGCAACCTACTGCAACTAGTAAAAACGGCCTTTAGAGATGTTTTAAGTGTTGAAGAGATTTCTGATGATGATGATTTCTTTACTATGGGTGGAAATTCTATTGCTGCTGCACATCTTTCTAATAATATAGGAGTTGATATGCGATTGATATATTGCTTTCCAAGTCCATCCATGCTTTGTACTGCTCTTTTAGAGAGAAAAGAATCATTGAATATAAACGTCAGTAGAGATGCTAAATCAAAGATGAATCTGGAAGGGGGTAAACCAAGTTTTTTTCATGTTCACTCTGACACTCCTGCTACAGTAAATTTTGATGAGCAGAGGAGATTACTAAGAACTCTTTCTGGAAGAAGTGAAGATAATGCAATTATCTCTAAACGCTTGAAACTGGATTCAAACATAAATGTTGCCGGTGATAGTAGTCCAGCAAATGGGTATCCATGGAATTCTGTGGCAATATATGCGTCTTGTTCTTTTAGCCGGTGCAACAAAGTTGTGTATGAAGGTGGGTCCGTGGTGAAAGATATTTATCAAGCAACCTGTTCTGTGATGGTCCCGAAAAGTAGAAATGTTCCTATGCAAGAATCATGGAAAGTTTACATGGGATTATGTGTTGATGCATCGCCAATTATTGTTCATAAAGGCCAGGATATCTACTTATTTATTGGATCGCACTCACATAAATTTATGTGTGTTAATGCCAGAAGTGGTTCTGTCCAGTGGGAGATAAAACTAGAAGGACGTGTGGAATGCTCAGCAGCAATTCTTAGTGACTTCACACAGGTTGTAGTTGGATGCTACAGAGGGAAAATATACTTCCTTGATTTTTTGAACGGTAACATCTGTTGGACTTTCCAAACATCTGGTGAGGTAAAGTCACAGCCCGTCATAGACAGTCAGAGACAATTGATCTGGTGTGGATCATATGACCATAACTTGTATGCTCTAGATTACAAAAACCATTGCTGTGTATATCAGCTTCCATGTGGTGGGAGTATCTATGGGTCACCTGCAATTGATGAGATGAATAATATACTTTATGTGGCTTCTACGAGTGGCCAAATGACAGCCATATCAATCGCGGCATTCCCATTTTCTATTCTGTGGCTGCATGGGCTAGAAGCGCCAGTGTTTGGTTCTCTTGCCATCAATTCTCTGAATGGAAATATTATATGTTGCTTAGTGGACGGTCATGTTCTCGCATTGGATACAAGTGGATCTGTTATTTGGAGGTATAGAACTGCTGGACCTATATTTGCTGGAGCCTGCATGTCTTCTGCTCTTCCTTTTCAGGCACTCATATGCTCTCGAGATGGAAGCATCTACTCACTTGAACTGGAAACGGGAGATTTACTCTGGCAATACAATGTTAAGGATCCAATTACTTCATCCGCTTATGTGGATGAGCACTTAAGCTTGGTATCTGATCGATCTAACTTGCCAGACAGGTTGGTTTGTATCTGTTCCAGCTCAGGAAGTGTATATTTGCTTCGGGTTAACTCGGGCGTCGCCAAAGTGGCAAATGAACCGATAGATGTTGAGGAATTTTCCAGACTGGACCTCGCAGGAGATGTATTTTCTTCACCTGTAATGATTGGTGGCAGAATTTTTGTTGGTTGTAGGGATGATTATATACACTGCATTACTGTGAAGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C PP-binding PQQ PQQ_2 PQQ_3

Protein Analysis

1155

Amino Acids

127.06

Weight (kDa)

5.79

Isoelectric Point (pI)

45.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1595
AarI CACCTGC 2 cut(s) 816, 2824
AasI GACNNNNNNGTC 1 cut(s) 1380
Acc36I ACCTGC 2 cut(s) 816, 2824
AccB7I CCANNNNNTGG 4 cut(s) 758, 2237, 2795, 2939
AccI GTMKAC 1 cut(s) 477
AccII CGCG 3 cut(s) 78, 555, 2888
AclWI GGATC 6 cut(s) 602, 2482, 2734, 3031, 3176, 3189
AcoI YGGCCR 4 cut(s) 96, 468, 1684, 2863
AcsI RAATTY 8 cut(s) 1093, 1260, 1900, 2113, 2251, 2489, 3350, 3411
AcyI GRCGYC 2 cut(s) 500, 3312
AfaI GTAC 8 cut(s) 371, 662, 844, 1108, 1129, 1224, 1462, 1987
AfeI AGCGCT 1 cut(s) 826
AfiI CCNNNNNNNGG 6 cut(s) 138, 444, 758, 2237, 2795, 2939
AflII CTTAAG 2 cut(s) 872, 3217
AflIII ACRYGT 1 cut(s) 2548
AhlI ACTAGT 1 cut(s) 1822
AjiI CACGTC 1 cut(s) 2549
AjnI CCWGG 3 cut(s) 494, 1681, 2451
AjuI GAANNNNNNNTTGG 4 cut(s) 1610, 1642, 1773, 1805
Alw21I GWGCWC 1 cut(s) 3216
Alw26I GTCTC 3 cut(s) 756, 1344, 2701
AlwI GGATC 6 cut(s) 602, 2482, 2734, 3031, 3176, 3189
Ama87I CYCGRG 3 cut(s) 536, 3114, 3307
Aor51HI AGCGCT 1 cut(s) 826
ApaI GGGCCC 1 cut(s) 145
ApoI RAATTY 8 cut(s) 1093, 1260, 1900, 2113, 2251, 2489, 3350, 3411
ArsI GACNNNNNNTTYG 2 cut(s) 1072, 1104
Asp700I GAANNNNTTC 3 cut(s) 1707, 2146, 2515
AspLEI GCGC 4 cut(s) 47, 827, 879, 2932
AspS9I GGNCC 9 cut(s) 141, 142, 424, 435, 1080, 2316, 2359, 3053, 3364
AsuII TTCGAA 1 cut(s) 513
AvaI CYCGRG 3 cut(s) 536, 3114, 3307
AvaII GGWCC 6 cut(s) 424, 1080, 2316, 2359, 3053, 3364
BaeGI GKGCMC 1 cut(s) 145
BalI TGGCCA 2 cut(s) 1686, 2865
BamHI GGATCC 1 cut(s) 3181
BanII GRGCYC 1 cut(s) 145
BarI GAAGNNNNNNTAC 4 cut(s) 2769, 2801, 3279, 3311
BauI CACGAG 1 cut(s) 1583
BbsI GAAGAC 1 cut(s) 3072
Bbv12I GWGCWC 1 cut(s) 3216
BceAI ACGGC 6 cut(s) 104, 241, 248, 487, 625, 1848
BciT130I CCWGG 3 cut(s) 496, 1683, 2453
BciVI GTATCC 5 cut(s) 759, 1255, 1286, 2253, 3007
BclI TGATCA 1 cut(s) 802
BcoDI GTCTC 3 cut(s) 756, 1344, 2701
BcuI ACTAGT 1 cut(s) 1822
BfaI CTAG 6 cut(s) 1722, 1823, 2540, 2753, 2924, 3463
BfmI CTRYAG 3 cut(s) 905, 2106, 2605
BfoI RGCGCY 2 cut(s) 828, 2933
BfrI CTTAAG 2 cut(s) 872, 3217
BfuAI ACCTGC 2 cut(s) 816, 2824
BfuI GTATCC 5 cut(s) 759, 1255, 1286, 2253, 3007
BglI GCCNNNNNGGC 1 cut(s) 3323
BlpI GCTNAGC 1 cut(s) 2560
BmcAI AGTACT 1 cut(s) 1108
Bme1390I CCNGG 3 cut(s) 496, 1683, 2453
Bme18I GGWCC 6 cut(s) 424, 1080, 2316, 2359, 3053, 3364
BmeT110I CYCGRG 3 cut(s) 536, 3114, 3307
BmgBI CACGTC 1 cut(s) 2549
BmgT120I GGNCC 9 cut(s) 141, 142, 424, 435, 1080, 2316, 2359, 3053, 3364
BmiI GGNNCC 6 cut(s) 143, 426, 2317, 2361, 3070, 3183
BmrFI CCNGG 3 cut(s) 496, 1683, 2453
BmrI ACTGGG 1 cut(s) 687
BmsI GCATC 9 cut(s) 4, 178, 726, 1528, 2026, 2413, 2435, 2591, 3135
BmuI ACTGGG 1 cut(s) 687
BpiI GAAGAC 1 cut(s) 3072
BplI GAGNNNNNCTC 2 cut(s) 3144, 3176
BpmI CTGGAG 2 cut(s) 478, 3087
Bpu10I CCTNAGC 1 cut(s) 3277
Bpu1102I GCTNAGC 1 cut(s) 2560
Bpu14I TTCGAA 1 cut(s) 513
BpuEI CTTGAG 1 cut(s) 1326
Bsa29I ATCGAT 2 cut(s) 597, 3235
BsaBI GATNNNNATC 1 cut(s) 2334
BsaHI GRCGYC 2 cut(s) 500, 3312
BsaJI CCNNGG 8 cut(s) 360, 465, 537, 998, 1076, 1617, 2246, 2319
BsaWI WCCGGW 1 cut(s) 134
BsaXI ACNNNNNCTCC 4 cut(s) 626, 656, 753, 783
Bsc4I CCNNNNNNNGG 6 cut(s) 138, 444, 758, 2237, 2795, 2939
Bse118I RCCGGY 2 cut(s) 2216, 2286
Bse1I ACTGG 8 cut(s) 682, 841, 1712, 2199, 2524, 2933, 3147, 3366
Bse3DI GCAATG 4 cut(s) 61, 886, 1680, 2767
Bse8I GATNNNNATC 1 cut(s) 2334
BseBI CCWGG 3 cut(s) 496, 1683, 2453
BseCI ATCGAT 2 cut(s) 597, 3235
BseDI CCNNGG 8 cut(s) 360, 465, 537, 998, 1076, 1617, 2246, 2319
BseGI GGATG 8 cut(s) 193, 741, 1656, 1973, 2606, 3194, 3214, 3436
BseJI GATNNNNATC 1 cut(s) 2334
BseLI CCNNNNNNNGG 6 cut(s) 138, 444, 758, 2237, 2795, 2939
BseMI GCAATG 4 cut(s) 61, 886, 1680, 2767
BseMII CTCAG 7 cut(s) 273, 696, 1101, 1539, 1770, 2574, 3291
BseNI ACTGG 8 cut(s) 682, 841, 1712, 2199, 2524, 2933, 3147, 3366
BseRI GAGGAG 3 cut(s) 206, 278, 2143
BseSI GKGCMC 1 cut(s) 145
BseYI CCCAGC 2 cut(s) 282, 326
BsgI GTGCAG 1 cut(s) 1899
Bsh1236I CGCG 3 cut(s) 78, 555, 2888
BshVI ATCGAT 2 cut(s) 597, 3235
BsiHKAI GWGCWC 1 cut(s) 3216
BsiHKCI CYCGRG 3 cut(s) 536, 3114, 3307
BsiSI CCGG 5 cut(s) 135, 208, 331, 2217, 2287
BslFI GGGAC 2 cut(s) 1533, 2345
BslI CCNNNNNNNGG 6 cut(s) 138, 444, 758, 2237, 2795, 2939
BsmAI GTCTC 3 cut(s) 756, 1344, 2701
BsmBI CGTCTC 1 cut(s) 756
BsmFI GGGAC 2 cut(s) 1533, 2345
BsmI GAATGC 2 cut(s) 2561, 2891
BsoBI CYCGRG 3 cut(s) 536, 3114, 3307
Bsp119I TTCGAA 1 cut(s) 513
Bsp120I GGGCCC 1 cut(s) 141
Bsp1286I GDGCHC 2 cut(s) 145, 3216
Bsp1407I TGTACA 2 cut(s) 660, 1127
Bsp143I GATC 9 cut(s) 594, 802, 2474, 2715, 2726, 3023, 3181, 3232, 3236
Bsp1720I GCTNAGC 1 cut(s) 2560
Bsp19I CCATGG 1 cut(s) 2246
BspCNI CTCAG 7 cut(s) 272, 697, 1102, 1538, 1769, 2573, 3290
BspDI ATCGAT 2 cut(s) 597, 3235
BspFNI CGCG 3 cut(s) 78, 555, 2888
BspLI GGNNCC 6 cut(s) 143, 426, 2317, 2361, 3070, 3183
BspMI ACCTGC 2 cut(s) 816, 2824
BspPI GGATC 6 cut(s) 602, 2482, 2734, 3031, 3176, 3189
BspQI GCTCTTC 2 cut(s) 570, 3093
BspT104I TTCGAA 1 cut(s) 513
BspTI CTTAAG 2 cut(s) 872, 3217
BsrDI GCAATG 4 cut(s) 61, 886, 1680, 2767
BsrFI RCCGGY 2 cut(s) 2216, 2286
BsrGI TGTACA 2 cut(s) 660, 1127
BsrI ACTGG 8 cut(s) 682, 841, 1712, 2199, 2524, 2933, 3147, 3366
BssAI RCCGGY 2 cut(s) 2216, 2286
BssECI CCNNGG 8 cut(s) 360, 465, 537, 998, 1076, 1617, 2246, 2319
BssMI GATC 9 cut(s) 594, 802, 2474, 2715, 2726, 3023, 3181, 3232, 3236
BssNI GRCGYC 2 cut(s) 500, 3312
BssSI CACGAG 1 cut(s) 1583
BssT1I CCWWGG 3 cut(s) 998, 1617, 2246
Bst2BI CACGAG 1 cut(s) 1583
Bst2UI CCWGG 3 cut(s) 496, 1683, 2453
Bst6I CTCTTC 3 cut(s) 570, 1854, 3093
BstACI GRCGYC 2 cut(s) 500, 3312
BstAFI CTTAAG 2 cut(s) 872, 3217
BstAPI GCANNNNNTGC 1 cut(s) 2267
BstAUI TGTACA 2 cut(s) 660, 1127
BstBI TTCGAA 1 cut(s) 513
BstC8I GCNNGC 4 cut(s) 129, 544, 613, 3073
BstDSI CCRYGG 2 cut(s) 2246, 2319
BstEII GGTNACC 1 cut(s) 2811
BstENI CCTNNNNNAGG 1 cut(s) 136
BstF5I GGATG 8 cut(s) 193, 741, 1656, 1973, 2606, 3194, 3214, 3436
BstFNI CGCG 3 cut(s) 78, 555, 2888
BstH2I RGCGCY 2 cut(s) 828, 2933
BstHHI GCGC 4 cut(s) 47, 827, 879, 2932
BstKTI GATC 9 cut(s) 597, 805, 2477, 2718, 2729, 3026, 3184, 3235, 3239
BstMAI GTCTC 3 cut(s) 756, 1344, 2701
BstMBI GATC 9 cut(s) 594, 802, 2474, 2715, 2726, 3023, 3181, 3232, 3236
BstNI CCWGG 3 cut(s) 496, 1683, 2453
BstNSI RCATGY 4 cut(s) 131, 355, 1778, 3079
BstPI GGTNACC 1 cut(s) 2811
BstSCI CCNGG 3 cut(s) 494, 1681, 2451
BstSFI CTRYAG 3 cut(s) 905, 2106, 2605
BstSLI GKGCMC 1 cut(s) 145
BstUI CGCG 3 cut(s) 78, 555, 2888
BstV2I GAAGAC 1 cut(s) 3072
BstX2I RGATCY 2 cut(s) 3023, 3181
BstXI CCANNNNNNTGG 1 cut(s) 2669
BstYI RGATCY 2 cut(s) 3023, 3181
Bsu15I ATCGAT 2 cut(s) 597, 3235
BsuI GTATCC 5 cut(s) 759, 1255, 1286, 2253, 3007
BsuTUI ATCGAT 2 cut(s) 597, 3235
BtgI CCRYGG 2 cut(s) 2246, 2319
BtgZI GCGATG 2 cut(s) 472, 2412
BtrI CACGTC 1 cut(s) 2549
BtsCI GGATG 8 cut(s) 193, 741, 1656, 1973, 2606, 3194, 3214, 3436
BtsI GCAGTG 1 cut(s) 3442
BtsIMutI CAGTG 7 cut(s) 752, 834, 1326, 1518, 2531, 2940, 3442
BveI ACCTGC 2 cut(s) 816, 2824
Cac8I GCNNGC 4 cut(s) 129, 544, 613, 3073
CfoI GCGC 4 cut(s) 47, 827, 879, 2932
Cfr10I RCCGGY 2 cut(s) 2216, 2286
Cfr13I GGNCC 9 cut(s) 141, 142, 424, 435, 1080, 2316, 2359, 3053, 3364
ClaI ATCGAT 2 cut(s) 597, 3235
CseI GACGC 3 cut(s) 508, 2259, 3301
Csp6I GTAC 8 cut(s) 370, 661, 843, 1107, 1128, 1223, 1461, 1986
CviQI GTAC 8 cut(s) 370, 661, 843, 1107, 1128, 1223, 1461, 1986
DpnI GATC 9 cut(s) 596, 804, 2476, 2717, 2728, 3025, 3183, 3234, 3238
DpnII GATC 9 cut(s) 594, 802, 2474, 2715, 2726, 3023, 3181, 3232, 3236
DrdI GACNNNNNNGTC 1 cut(s) 1380
DseDI GACNNNNNNGTC 1 cut(s) 1380
EaeI YGGCCR 4 cut(s) 96, 468, 1684, 2863
Eam1104I CTCTTC 3 cut(s) 570, 1854, 3093
EarI CTCTTC 3 cut(s) 570, 1854, 3093
EciI GGCGGA 1 cut(s) 380
Eco130I CCWWGG 3 cut(s) 998, 1617, 2246
Eco147I AGGCCT 3 cut(s) 410, 542, 3461
Eco24I GRGCYC 1 cut(s) 145
Eco32I GATATC 1 cut(s) 2458
Eco47I GGWCC 6 cut(s) 424, 1080, 2316, 2359, 3053, 3364
Eco47III AGCGCT 1 cut(s) 826
Eco88I CYCGRG 3 cut(s) 536, 3114, 3307
Eco91I GGTNACC 1 cut(s) 2811
EcoNI CCTNNNNNAGG 1 cut(s) 136
EcoO109I RGGNCCY 2 cut(s) 141, 142
EcoO65I GGTNACC 1 cut(s) 2811
EcoRI GAATTC 1 cut(s) 2251
EcoRII CCWGG 3 cut(s) 494, 1681, 2451
EcoRV GATATC 1 cut(s) 2458
EcoT14I CCWWGG 3 cut(s) 998, 1617, 2246
EcoT22I ATGCAT 1 cut(s) 2428
EcoT38I GRGCYC 1 cut(s) 145
ErhI CCWWGG 3 cut(s) 998, 1617, 2246
Esp3I CGTCTC 1 cut(s) 756
FalI AAGNNNNNCTT 4 cut(s) 804, 836, 1773, 1805
FaqI GGGAC 2 cut(s) 1533, 2345
FauI CCCGC 2 cut(s) 83, 176
FauNDI CATATG 2 cut(s) 2731, 3107
FbaI TGATCA 1 cut(s) 802
FblI GTMKAC 1 cut(s) 477
FokI GGATG 8 cut(s) 200, 748, 1663, 1960, 2613, 3181, 3221, 3443
FriOI GRGCYC 1 cut(s) 145
FspBI CTAG 6 cut(s) 1722, 1823, 2540, 2753, 2924, 3463
GlaI GCGC 4 cut(s) 46, 826, 878, 2931
GsaI CCCAGC 2 cut(s) 286, 330
GsuI CTGGAG 2 cut(s) 478, 3087
HaeII RGCGCY 2 cut(s) 828, 2933
HapII CCGG 5 cut(s) 135, 208, 331, 2217, 2287
HgaI GACGC 3 cut(s) 508, 2259, 3301
HhaI GCGC 4 cut(s) 47, 827, 879, 2932
Hin1I GRCGYC 2 cut(s) 500, 3312
Hin6I GCGC 4 cut(s) 45, 825, 877, 2930
HinP1I GCGC 4 cut(s) 45, 825, 877, 2930
HincII GTYRAC 2 cut(s) 478, 3304
HindII GTYRAC 2 cut(s) 478, 3304
HindIII AAGCTT 2 cut(s) 793, 3220
HpaI GTTAAC 1 cut(s) 3304
HpaII CCGG 5 cut(s) 135, 208, 331, 2217, 2287
Hpy166II GTNNAC 8 cut(s) 272, 478, 663, 2069, 2089, 2404, 2992, 3304
Hpy8I GTNNAC 8 cut(s) 272, 478, 663, 2069, 2089, 2404, 2992, 3304
Hpy99I CGWCG 2 cut(s) 269, 3317
HpyAV CCTTC 9 cut(s) 247, 438, 625, 971, 975, 2054, 2303, 2537, 3451
HpyCH4IV ACGT 3 cut(s) 1797, 2025, 2548
HpySE526I ACGT 3 cut(s) 1797, 2025, 2548
Hsp92I GRCGYC 2 cut(s) 500, 3312
HspAI GCGC 4 cut(s) 45, 825, 877, 2930
Ksp22I TGATCA 1 cut(s) 802
KspAI GTTAAC 1 cut(s) 3304
Kzo9I GATC 9 cut(s) 594, 802, 2474, 2715, 2726, 3023, 3181, 3232, 3236
LguI GCTCTTC 2 cut(s) 570, 3093
LmnI GCTCC 5 cut(s) 199, 219, 291, 1631, 3068
LweI GCATC 9 cut(s) 4, 178, 726, 1528, 2026, 2413, 2435, 2591, 3135
MaeI CTAG 6 cut(s) 1722, 1823, 2540, 2753, 2924, 3463
MaeII ACGT 3 cut(s) 1797, 2025, 2548
MaeIII GTNAC 9 cut(s) 1071, 1312, 1430, 1696, 1793, 2576, 2642, 2682, 2811
MalI GATC 9 cut(s) 596, 804, 2476, 2717, 2728, 3025, 3183, 3234, 3238
MboI GATC 9 cut(s) 594, 802, 2474, 2715, 2726, 3023, 3181, 3232, 3236
MfeI CAATTG 3 cut(s) 1191, 2711, 2820
MflI RGATCY 2 cut(s) 3023, 3181
MhlI GDGCHC 2 cut(s) 145, 3216
MlsI TGGCCA 2 cut(s) 1686, 2865
MluNI TGGCCA 2 cut(s) 1686, 2865
MlyI GAGTC 2 cut(s) 515, 1748
MmeI TCCRAC 3 cut(s) 1628, 2578, 2633
Mox20I TGGCCA 2 cut(s) 1686, 2865
Mph1103I ATGCAT 1 cut(s) 2428
MroXI GAANNNNTTC 3 cut(s) 1707, 2146, 2515
MscI TGGCCA 2 cut(s) 1686, 2865
MslI CAYNNNNRTG 3 cut(s) 2772, 3106, 3452
Msp20I TGGCCA 2 cut(s) 1686, 2865
MspA1I CMGCKG 1 cut(s) 95
MspCI CTTAAG 2 cut(s) 872, 3217
MspI CCGG 5 cut(s) 135, 208, 331, 2217, 2287
MspR9I CCNGG 3 cut(s) 496, 1683, 2453
MunI CAATTG 3 cut(s) 1191, 2711, 2820
Mva1269I GAATGC 2 cut(s) 2561, 2891
MvaI CCWGG 3 cut(s) 496, 1683, 2453
MvnI CGCG 3 cut(s) 78, 555, 2888
NcoI CCATGG 1 cut(s) 2246
NdeI CATATG 2 cut(s) 2731, 3107
NdeII GATC 9 cut(s) 594, 802, 2474, 2715, 2726, 3023, 3181, 3232, 3236
NlaIV GGNNCC 6 cut(s) 143, 426, 2317, 2361, 3070, 3183
NmeAIII GCCGAG 2 cut(s) 341, 446
NmuCI GTSAC 5 cut(s) 1430, 1793, 2576, 2682, 2811
NsiI ATGCAT 1 cut(s) 2428
NspI RCATGY 4 cut(s) 131, 355, 1778, 3079
NspV TTCGAA 1 cut(s) 513
PaeI GCATGC 1 cut(s) 131
PaeR7I CTCGAG 1 cut(s) 3114
PaqCI CACCTGC 2 cut(s) 816, 2824
PceI AGGCCT 3 cut(s) 410, 542, 3461
PciSI GCTCTTC 2 cut(s) 570, 3093
PctI GAATGC 2 cut(s) 2561, 2891
PdmI GAANNNNTTC 3 cut(s) 1707, 2146, 2515
PflMI CCANNNNNTGG 4 cut(s) 758, 2237, 2795, 2939
PfoI TCCNGGA 1 cut(s) 494
PleI GAGTC 2 cut(s) 515, 1748
PpsI GAGTC 2 cut(s) 515, 1748
PsiI TTATAA 1 cut(s) 1595
Psp6I CCWGG 3 cut(s) 494, 1681, 2451
PspEI GGTNACC 1 cut(s) 2811
PspFI CCCAGC 2 cut(s) 282, 326
PspGI CCWGG 3 cut(s) 494, 1681, 2451
PspN4I GGNNCC 6 cut(s) 143, 426, 2317, 2361, 3070, 3183
PspOMI GGGCCC 1 cut(s) 141
PspPI GGNCC 9 cut(s) 141, 142, 424, 435, 1080, 2316, 2359, 3053, 3364
PsrI GAACNNNNNNTAC 2 cut(s) 1563, 1595
PsuI RGATCY 2 cut(s) 3023, 3181
RsaI GTAC 8 cut(s) 371, 662, 844, 1108, 1129, 1224, 1462, 1987
RsaNI GTAC 8 cut(s) 370, 661, 843, 1107, 1128, 1223, 1461, 1986
RseI CAYNNNNRTG 3 cut(s) 2772, 3106, 3452
SalI GTCGAC 1 cut(s) 476
SapI GCTCTTC 2 cut(s) 570, 3093
Sau3AI GATC 9 cut(s) 594, 802, 2474, 2715, 2726, 3023, 3181, 3232, 3236
Sau96I GGNCC 9 cut(s) 141, 142, 424, 435, 1080, 2316, 2359, 3053, 3364
ScaI AGTACT 1 cut(s) 1108
SchI GAGTC 2 cut(s) 515, 1748
ScrFI CCNGG 3 cut(s) 496, 1683, 2453
SduI GDGCHC 2 cut(s) 145, 3216
SfaNI GCATC 9 cut(s) 4, 178, 726, 1528, 2026, 2413, 2435, 2591, 3135
SfcI CTRYAG 3 cut(s) 905, 2106, 2605
Sfr274I CTCGAG 1 cut(s) 3114
SfuI TTCGAA 1 cut(s) 513
SinI GGWCC 6 cut(s) 424, 1080, 2316, 2359, 3053, 3364
SlaI CTCGAG 1 cut(s) 3114
SmiMI CAYNNNNRTG 3 cut(s) 2772, 3106, 3452
SmlI CTYRAG 4 cut(s) 872, 1341, 3114, 3217
SmoI CTYRAG 4 cut(s) 872, 1341, 3114, 3217
SpeI ACTAGT 1 cut(s) 1822
SphI GCATGC 1 cut(s) 131
SseBI AGGCCT 3 cut(s) 410, 542, 3461
SspI AATATT 4 cut(s) 1388, 1599, 1626, 2974
SspMI CTAG 6 cut(s) 1722, 1823, 2540, 2753, 2924, 3463
StuI AGGCCT 3 cut(s) 410, 542, 3461
StyD4I CCNGG 3 cut(s) 494, 1681, 2451
StyI CCWWGG 3 cut(s) 998, 1617, 2246
TaiI ACGT 3 cut(s) 1800, 2028, 2551
TaqII GACCGA 1 cut(s) 1188
TatI WGTACW 6 cut(s) 369, 660, 842, 1106, 1127, 1985
TauI GCSGC 5 cut(s) 98, 358, 1029, 1169, 2891
TscAI CASTG 7 cut(s) 759, 841, 1333, 1518, 2531, 2940, 3449
TseFI GTSAC 5 cut(s) 1430, 1793, 2576, 2682, 2811
Tsp45I GTSAC 5 cut(s) 1430, 1793, 2576, 2682, 2811
TspDTI ATGAA 8 cut(s) 1544, 2066, 2072, 2322, 2432, 2846, 3183, 3345
TspGWI ACGGA 3 cut(s) 152, 370, 2308
TspRI CASTG 7 cut(s) 759, 841, 1333, 1518, 2531, 2940, 3449
Van91I CCANNNNNTGG 4 cut(s) 758, 2237, 2795, 2939
Vha464I CTTAAG 2 cut(s) 872, 3217
VpaK11BI GGWCC 6 cut(s) 424, 1080, 2316, 2359, 3053, 3364
XagI CCTNNNNNAGG 1 cut(s) 136
XapI RAATTY 8 cut(s) 1093, 1260, 1900, 2113, 2251, 2489, 3350, 3411
XbaI TCTAGA 1 cut(s) 2752
XceI RCATGY 4 cut(s) 131, 355, 1778, 3079
XhoI CTCGAG 1 cut(s) 3114
XmiI GTMKAC 1 cut(s) 477
XmnI GAANNNNTTC 3 cut(s) 1707, 2146, 2515
XspI CTAG 6 cut(s) 1722, 1823, 2540, 2753, 2924, 3463
ZrmI AGTACT 1 cut(s) 1108
Zsp2I ATGCAT 1 cut(s) 2428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.