Rmu_sc0002160.1_g000019

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002160.1
OG0001590
Physical Location & Seq
Forward (+)
93937 .. 96821
2885 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002160.1_g000019.1.cds

Sequence Viewer

Length: 495 bp
atgcaggcattcccattttctattatgtgggtgcatgaattagaagtgccagtatttgggtctcttgccatcaattctctgaatggaaatattatatgttgcttggtggatgggcatgttcttgcatttactacaagtggatctattatttggagggaatcgggaggtttactctgggaatacaatgttaaggatccaattacttcatctgcctatgtcgatgagcacctgagcttggtgtctgatcagtctaactttcccgaccgcagctccggaagcatggttctgattcaggttaatttggatgccaccgaaggtgcaagtccaatgaatttcaatgttgaggaatttgcaaggttggaactcccaggagatgtattctcttcccctgtaatgattggtggcagaattttcgttggttgtagggatgattatgtgcactgcattgctgttaagccccaggtctctacaggaacaatgagttgttctgtatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C PP-binding PQQ PQQ_2 PQQ_3

Protein Analysis

164

Amino Acids

17.84

Weight (kDa)

4.46

Isoelectric Point (pI)

37.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 56
AccIII TCCGGA 1 cut(s) 272
AciI CCGC 1 cut(s) 265
AclWI GGATC 3 cut(s) 148, 188, 201
AcsI RAATTY 3 cut(s) 331, 347, 408
AfiI CCNNNNNNNGG 2 cut(s) 56, 235
AgsI TTSAA 1 cut(s) 337
AjnI CCWGG 2 cut(s) 367, 459
AloI GAACNNNNNNTCC 2 cut(s) 267, 299
AluBI AGCT 2 cut(s) 234, 270
AluI AGCT 2 cut(s) 234, 270
Alw21I GWGCWC 2 cut(s) 228, 441
Alw26I GTCTC 2 cut(s) 66, 469
Alw44I GTGCAC 1 cut(s) 437
AlwI GGATC 3 cut(s) 148, 188, 201
Aor13HI TCCGGA 1 cut(s) 272
ApaLI GTGCAC 1 cut(s) 437
ApeKI GCWGC 1 cut(s) 267
ApoI RAATTY 3 cut(s) 331, 347, 408
BaeGI GKGCMC 1 cut(s) 441
BamHI GGATCC 1 cut(s) 193
Bbv12I GWGCWC 2 cut(s) 228, 441
BbvI GCAGC 1 cut(s) 279
BccI CCATC 2 cut(s) 77, 104
BcgI CGANNNNNNTGC 2 cut(s) 394, 428
BciT130I CCWGG 2 cut(s) 369, 461
BclI TGATCA 1 cut(s) 244
BcoDI GTCTC 2 cut(s) 66, 469
BfmI CTRYAG 1 cut(s) 468
BisI GCNGC 1 cut(s) 268
BlsI GCNGC 1 cut(s) 269
Bme1390I CCNGG 2 cut(s) 369, 461
BmiI GGNNCC 1 cut(s) 195
BmrFI CCNGG 2 cut(s) 369, 461
BmsI GCATC 1 cut(s) 295
BplI GAGNNNNNCTC 2 cut(s) 156, 188
Bpu10I CCTNAGC 1 cut(s) 230
BsaI GGTCTC 2 cut(s) 66, 469
BsaJI CCNNGG 2 cut(s) 367, 459
BsaWI WCCGGW 1 cut(s) 272
BsaXI ACNNNNNCTCC 2 cut(s) 254, 284
Bsc4I CCNNNNNNNGG 2 cut(s) 56, 235
Bse1I ACTGG 1 cut(s) 50
Bse3DI GCAATG 1 cut(s) 444
BseAI TCCGGA 1 cut(s) 272
BseBI CCWGG 2 cut(s) 369, 461
BseDI CCNNGG 2 cut(s) 367, 459
BseGI GGATG 3 cut(s) 115, 310, 433
BseLI CCNNNNNNNGG 2 cut(s) 56, 235
BseMI GCAATG 1 cut(s) 444
BseMII CTCAG 1 cut(s) 221
BseNI ACTGG 1 cut(s) 50
BseSI GKGCMC 1 cut(s) 441
BseXI GCAGC 1 cut(s) 279
Bsh1285I CGRYCG 1 cut(s) 265
BsiEI CGRYCG 1 cut(s) 265
BsiHKAI GWGCWC 2 cut(s) 228, 441
BsiSI CCGG 1 cut(s) 273
BslI CCNNNNNNNGG 2 cut(s) 56, 235
BsmAI GTCTC 2 cut(s) 66, 469
BsmI GAATGC 1 cut(s) 8
Bso31I GGTCTC 2 cut(s) 66, 469
Bsp1286I GDGCHC 2 cut(s) 228, 441
Bsp13I TCCGGA 1 cut(s) 272
Bsp143I GATC 3 cut(s) 140, 193, 244
BspACI CCGC 1 cut(s) 265
BspCNI CTCAG 1 cut(s) 222
BspEI TCCGGA 1 cut(s) 272
BspLI GGNNCC 1 cut(s) 195
BspPI GGATC 3 cut(s) 148, 188, 201
BspTNI GGTCTC 2 cut(s) 66, 469
BsrDI GCAATG 1 cut(s) 444
BsrI ACTGG 1 cut(s) 50
BssECI CCNNGG 2 cut(s) 367, 459
BssMI GATC 3 cut(s) 140, 193, 244
Bst2UI CCWGG 2 cut(s) 369, 461
Bst6I CTCTTC 1 cut(s) 388
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 1 cut(s) 230
BstF5I GGATG 3 cut(s) 115, 310, 433
BstKTI GATC 3 cut(s) 143, 196, 247
BstMAI GTCTC 2 cut(s) 66, 469
BstMBI GATC 3 cut(s) 140, 193, 244
BstMCI CGRYCG 1 cut(s) 265
BstMWI GCNNNNNNNGC 1 cut(s) 276
BstNI CCWGG 2 cut(s) 369, 461
BstNSI RCATGY 1 cut(s) 119
BstSCI CCNGG 2 cut(s) 367, 459
BstSFI CTRYAG 1 cut(s) 468
BstSLI GKGCMC 1 cut(s) 441
BstV1I GCAGC 1 cut(s) 279
BstX2I RGATCY 2 cut(s) 140, 193
BstYI RGATCY 2 cut(s) 140, 193
BtsCI GGATG 3 cut(s) 115, 310, 433
BtsI GCAGTG 1 cut(s) 439
BtsIMutI CAGTG 1 cut(s) 439
Cac8I GCNNGC 1 cut(s) 6
CviAII CATG 3 cut(s) 35, 116, 280
CviJI RGCY 3 cut(s) 234, 270, 457
CviKI_1 RGCY 3 cut(s) 234, 270, 457
DdeI CTNAG 1 cut(s) 230
DpnI GATC 3 cut(s) 142, 195, 246
DpnII GATC 3 cut(s) 140, 193, 244
Eam1104I CTCTTC 1 cut(s) 388
EarI CTCTTC 1 cut(s) 388
Eco31I GGTCTC 2 cut(s) 66, 469
EcoRII CCWGG 2 cut(s) 367, 459
FaeI CATG 3 cut(s) 38, 119, 283
FaiI YATR 9 cut(s) 26, 36, 95, 97, 117, 216, 281, 435, 493
FatI CATG 3 cut(s) 34, 115, 279
FbaI TGATCA 1 cut(s) 244
Fnu4HI GCNGC 1 cut(s) 268
FokI GGATG 3 cut(s) 122, 317, 440
Fsp4HI GCNGC 1 cut(s) 268
GluI GCNGC 1 cut(s) 268
HapII CCGG 1 cut(s) 273
Hin1II CATG 3 cut(s) 38, 119, 283
HinfI GANTC 2 cut(s) 158, 289
HpaII CCGG 1 cut(s) 273
Hpy166II GTNNAC 2 cut(s) 170, 439
Hpy188I TCNGA 3 cut(s) 81, 244, 288
Hpy188III TCNNGA 3 cut(s) 162, 260, 273
Hpy8I GTNNAC 2 cut(s) 170, 439
HpyAV CCTTC 1 cut(s) 308
HpyCH4V TGCA 7 cut(s) 4, 34, 125, 320, 353, 439, 444
HpyF10VI GCNNNNNNNGC 1 cut(s) 276
HpyF3I CTNAG 1 cut(s) 230
Hsp92II CATG 3 cut(s) 38, 119, 283
Kpn2I TCCGGA 1 cut(s) 272
Ksp22I TGATCA 1 cut(s) 244
Kzo9I GATC 3 cut(s) 140, 193, 244
LmnI GCTCC 1 cut(s) 275
Lsp1109I GCAGC 1 cut(s) 279
LweI GCATC 1 cut(s) 295
MalI GATC 3 cut(s) 142, 195, 246
MboI GATC 3 cut(s) 140, 193, 244
MboII GAAGA 1 cut(s) 375
MflI RGATCY 2 cut(s) 140, 193
MhlI GDGCHC 2 cut(s) 228, 441
MluCI AATT 7 cut(s) 38, 73, 198, 298, 331, 347, 408
MmeI TCCRAC 1 cut(s) 339
MnlI CCTC 3 cut(s) 147, 158, 337
MroI TCCGGA 1 cut(s) 272
MseI TTAA 3 cut(s) 189, 297, 453
MspI CCGG 1 cut(s) 273
MspR9I CCNGG 2 cut(s) 369, 461
Mva1269I GAATGC 1 cut(s) 8
MvaI CCWGG 2 cut(s) 369, 461
MwoI GCNNNNNNNGC 1 cut(s) 276
NdeII GATC 3 cut(s) 140, 193, 244
NlaIII CATG 3 cut(s) 38, 119, 283
NlaIV GGNNCC 1 cut(s) 195
NspI RCATGY 1 cut(s) 119
PctI GAATGC 1 cut(s) 8
PfeI GAWTC 2 cut(s) 158, 289
PflMI CCANNNNNTGG 1 cut(s) 56
PkrI GCNGC 1 cut(s) 269
Psp6I CCWGG 2 cut(s) 367, 459
PspGI CCWGG 2 cut(s) 367, 459
PspN4I GGNNCC 1 cut(s) 195
PsuI RGATCY 2 cut(s) 140, 193
SaqAI TTAA 3 cut(s) 189, 297, 453
SatI GCNGC 1 cut(s) 268
Sau3AI GATC 3 cut(s) 140, 193, 244
ScrFI CCNGG 2 cut(s) 369, 461
SduI GDGCHC 2 cut(s) 228, 441
SetI ASST 8 cut(s) 169, 231, 236, 272, 297, 319, 359, 465
SfaNI GCATC 1 cut(s) 295
SfcI CTRYAG 1 cut(s) 468
Sse9I AATT 7 cut(s) 38, 73, 198, 298, 331, 347, 408
SsiI CCGC 1 cut(s) 265
SspI AATATT 1 cut(s) 91
StyD4I CCNGG 2 cut(s) 367, 459
TaqI TCGA 1 cut(s) 219
TasI AATT 7 cut(s) 38, 73, 198, 298, 331, 347, 408
TfiI GAWTC 2 cut(s) 158, 289
Tru1I TTAA 3 cut(s) 189, 297, 453
Tru9I TTAA 3 cut(s) 189, 297, 453
TscAI CASTG 1 cut(s) 446
TseI GCWGC 1 cut(s) 267
TspDTI ATGAA 3 cut(s) 51, 195, 344
TspRI CASTG 1 cut(s) 446
Van91I CCANNNNNTGG 1 cut(s) 56
VneI GTGCAC 1 cut(s) 437
XapI RAATTY 3 cut(s) 331, 347, 408
XceI RCATGY 1 cut(s) 119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.