Rh1BG120800

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0001590
Physical Location & Seq
Reverse (-)
20511341 .. 20530728
19388 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG120800.1

Sequence Viewer

Length: 3354 bp
ATGAGAGTAATCCTTCCAGCTTTGCAAGGTCGAGACTATCAGCTTCTAAGTTCACTGAATTTGTTAGTGCTAAGCGGCGAAATTTTGCCTTTATCCTTGTGGGATATGCTGTCAAAGAGATTACCAAGGACCTCTATTTTGAATCTGTATGGGAGTACGGAGGTGTCAGGTGATTGTACATATTTTGACTGCAAGAGGTTGCCAATGATTTTGGAGACAGAGACGCTAACAAGTGTTCCAATTGGTATACCTATAGCTGGTTGTGATGTTGTGCTTGTTAGTGATGATGATGTACTCAATGAAGGAGAAATTTTTGTTGCTGGTCTCTGCAATGCTTCTGGATACTATTCTGATTCTACATTTACTCCTTTGACCACCGTAAAATTACCTCCAGATTCTGTTTGTTCCGGTTCTGTAAATGGACACGAAAGTCAATCATATTTTCAAACTGGTGATTTTGCCAAACAACTTCATAGCAGTGACTTGGTTTTCTTGGGAAGGAAGGACCGCACTATTAAGCTCAATGGGCAGCGTATTGCTTTAGAAGAAATTGAACATACATTGGTGGGACATCCAGATGTAACTGATGCTGCTGTTGTTTTTCATAAAGGTCAAGGGGAACTTATGCAACTTGTAGCATTTATAATGTTAAAGGAGGGAAGACCTGATGAAATATTCAGATCTTCCATCAAAAGTTGGATGGTTGACAAACTTCCACTGGCAATGATTCCTGGCCACTTTGTTATAATGGAATCATTTCCTGTATCTACTAGTGGAAAAGTTGACTATGCTTTGTTAGCAGATTCAGTATTTCTAGCAAAACATATCCAAGATGAGCTTGGTCATATTGGGAGAAGCAATCTACTGCAAGTAATTAAAAAGGCCTTTAGTCGTGTTTTAATGGCTGAAGAGGTTTCTGATGATGATGATTTCTTTATGATTGGTGGAAATTCTATTGCTGCTGCACATCTTGCTAACAACATAGGAGTTGATATGAGATTGATATATAGTTTTCCTACTCCATCTAAGCTTTCTATGGCTCTTCTAGAGAGAAAAGAACCATTCAGTATGAAAGTTAAAGTGGATTCTGAATCTGAGATGAATCAGGAAAGGGGAAAGAACATGTTTCATGTCCTCTATGACACTCCTAGTGCTGTTAATCTTGAACAGTTGAAAACTCTGCCTAGAAGGAATGAAAATAAAGCAGTTATGTCTAAACGCTTGAAAGCAGATTCAAGTATAAATGTTGCTTCAGGGAGTACTAGTCCAGCAGATGGGTATCCATGGAATTCTGTGCAAAAATGTATGACATGTTCTTTCAGCAGGTGCAACAAAGTTGTGTATGGGGGCAGTTCCAGGGTGAATGATGTTTATCAAGCGGCTAGTTCTGTGGTTGTCCCAAAAAGTCGAAAAGTGCATTTGGAAGAATTGTGGAAAGTTGACATGGGATCCTGCGTTGACGCATCACCACTTATTGTTTGTAAAGGCCAGGATATCTACTTATTTATTGGATCACACTCACAAAAGTTTGTGTGTGTTAATGCCAGAAGTGGTTCCATCCAGTGGGAGTTACAACTCGAAGGACGTGTAGAATGTTCAGCAGCAATTCTTGGTGACTTTACTCAGGTTGTAGTTGGATGCTACAAAGGGAAGATATATTTCCTGGATTCTTTGAATGGTAACATAAGTTGGACTTTCCAAACGTCTGGTGAGGTAAAGTCACAGCCGGTTATAGACAGTCAAAGACAATTGATTTGGTGTGGGTCATATGACCATAACTTGTATGCGCTAGATTATAAAAATCATTGCTGTGTTTATCGGCTTCCATGTGGTGGGAGTATCTGTGGGTCTCCTGCAATAGATGAGATGAATAATGTACTTTATGTGGCTTCTACTGGAGGCCAAATGACAGCCATTTCAATAACGGCATTCCCGTTTTCTATTATGTGGGTGCATGAGTTAGAAGTGCCAGTATTTGGGTCTCTTGTCATCAATTCTCTGAATGGAAATATTATATGTTGCTTGGTGGATGGGCATGTTCTTGCATTTAATACAAGTGGATCTATTATTTGGAGGTATAGAACTTCTGGGCCTGTATTTGCTGGAGCCTGCATATCTTCTGCTCTTCCCTTTCAGCTACTTGTCTGTTCCAGAGACGGAAACATCTATTCACTCGAACTCGAATCGGGAGGTTTACTCTGGGAATACAATGTAAAGGATCCAATTACTTCATCTGCCTATGTCGATGAGCACCTGAGCTTGGTGTCTGATCAGTCTAACTTTCCCGACCGCAGGGTGGTTTGTGTATGTTCCAGCTCTGGAAGCATGGTTCTGATTCAGGTTAATTTGGATGCCACTGAAGGTGCAAGTCCAATGAATTTCAATGTTGAGGAATTAGCAAGGTTGGAACTCCCAGGAGATGTATTCTCTTCCCCTGTAATGATTGGTGGCAGAATTTTCGTTGGTTGTAGGGATGATTATGTGCACTGCATTGCTGTTAAGCCCCAGGTACTTCTGTCCATCAACTGTCTTGGTAGTAACTCAACCGATAACAGTACTTACCAGAGAAACCTCAATACCCTCCTCCCTACTCTGCCCTCTGCCGGCAACGGCTACGGTTTTTACAACTCCTCTCTCGGCGAAAATGAAGACAAAGTCTATGCGGTTGCACTCTGCAGACCATTTGTTGAGCCGGATGCCTGTATCGATTGCCTCAGTTTCGCTAGAGACAAGATCAAGCTGGCATGCCCTCTTCAGCAGGAGGCTGTTGCATGGTACGACAATTGCACTTTACAGTACTCAGACAAGTCCATCTACGGCATCATGGAAACTACTCCTTCTTACAGTAGATGGAACGCACAAAACGTATCAACGCCAGATGTGGATGGGTTCTACAACCAGCTCATAGCCTTGATGGAAAGACTAAGTGGTCAAGCCGCGGCGAATGGTCCTCTTCTCAAGTGTGCAGTGGGAAATTTAACCCCTCCAGGTATATATCAAACAATATATACCTTCCTGCAGTGCAATCCAGATTTGTCCCCGCAAGACTGCAGTGATTGCTTAACATCAGCTCGGTTCGATGTCAGAAATTATTTCTTCGGAAAAAAAATTGGGGTGATCATTTACGCCAGCTGCACCATAAAAGTTCTGGATTCTGTATTTTATGAACCTGCAAGTATCGAGGCACTATTACTATCAGCGAATGGTACAACTTCAGGAAATACTACTACAATAACTTCAGTAAACCTTGCTGCAGCTCCAGGAGGAAGTCCAGGAAAGAAGCAGAGTAATAAATCTCGGACTATAATCATTATTGTAGTGGTAATTGTTGTTTCTCTGGCTCATCCGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C PP-binding PQQ PQQ_2 PQQ_3

Protein Analysis

1117

Amino Acids

122.1

Weight (kDa)

5.39

Isoelectric Point (pI)

40.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 12 - 116 1.3e-13 AMP-binding enzyme
AMP-binding_C PF13193 183 - 260 3.6e-09 AMP-binding enzyme C-terminal domain
PQQ_2 PF13360 475 - 598 7.5e-09 Outer membrane protein assembly factor BamB
Beta-prop_ACSF4 PF13570 482 - 832 7.7e-103 Beta-alanine-activating enzyme, beta-propeller
PQQ_2 PF13360 624 - 831 5.3e-11 Outer membrane protein assembly factor BamB
Stress-antifung PF01657 846 - 935 1.1e-21 Salt stress response/antifungal
Stress-antifung PF01657 962 - 1048 2e-06 Salt stress response/antifungal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 644, 746, 1797
AarI CACCTGC 1 cut(s) 1314
AasI GACNNNNNNGTC 2 cut(s) 429, 2928
Acc36I ACCTGC 2 cut(s) 1314, 3178
AccB7I CCANNNNNTGG 4 cut(s) 1274, 1563, 1832, 1976
AccI GTMKAC 1 cut(s) 247
AccII CGCG 1 cut(s) 2939
AciI CCGC 8 cut(s) 75, 508, 1379, 2290, 2663, 2937, 2939, 3041
AclWI GGATC 6 cut(s) 1443, 1456, 1519, 2068, 2213, 2226
AcoI YGGCCR 1 cut(s) 733
AcsI RAATTY 8 cut(s) 58, 81, 309, 949, 1288, 2377, 2454, 2974
AcuI CTGAAG 6 cut(s) 927, 1236, 2379, 2738, 3198, 3222
AfiI CCNNNNNNNGG 9 cut(s) 257, 1274, 1563, 1832, 1976, 2260, 2292, 2296, 2603
AflIII ACRYGT 3 cut(s) 1122, 1310, 1585
AhlI ACTAGT 2 cut(s) 770, 1262
AjiI CACGTC 1 cut(s) 1586
AjnI CCWGG 9 cut(s) 730, 1355, 1488, 1662, 2413, 2505, 2986, 3259, 3271
AloI GAACNNNNNNTCC 2 cut(s) 2313, 2345
Alw21I GWGCWC 2 cut(s) 2253, 2487
Alw26I GTCTC 8 cut(s) 27, 209, 215, 329, 1854, 1986, 2148, 2721
Alw44I GTGCAC 1 cut(s) 2483
AlwI GGATC 6 cut(s) 1443, 1456, 1519, 2068, 2213, 2226
AlwNI CAGNNNCTG 1 cut(s) 398
AoxI GGCC 5 cut(s) 733, 882, 1486, 1900, 2090
ApaLI GTGCAC 1 cut(s) 2483
ApeKI GCWGC 8 cut(s) 529, 590, 959, 962, 1601, 3132, 3251, 3254
ApoI RAATTY 8 cut(s) 58, 81, 309, 949, 1288, 2377, 2454, 2974
ArsI GACNNNNNNTTYG 4 cut(s) 121, 153, 1737, 1769
Asp700I GAANNNNTTC 2 cut(s) 756, 1552
AspLEI GCGC 1 cut(s) 1789
AspS9I GGNCC 4 cut(s) 129, 505, 2090, 2948
AsuHPI GGTGA 7 cut(s) 182, 464, 1372, 1458, 1625, 1721, 3127
AvaII GGWCC 3 cut(s) 129, 505, 2948
BaeGI GKGCMC 1 cut(s) 2487
BaeI ACNNNNGTAYC 2 cut(s) 2501, 2534
BalI TGGCCA 1 cut(s) 735
BamHI GGATCC 2 cut(s) 1448, 2218
BbsI GAAGAC 2 cut(s) 667, 2655
Bbv12I GWGCWC 2 cut(s) 2253, 2487
BbvI GCAGC 8 cut(s) 541, 577, 946, 949, 1613, 3119, 3238, 3266
BceAI ACGGC 3 cut(s) 1941, 2626, 2833
BcgI CGANNNNNNTGC 2 cut(s) 2440, 2474
BciT130I CCWGG 9 cut(s) 732, 1357, 1490, 1664, 2415, 2507, 2988, 3261, 3273
BciVI GTATCC 2 cut(s) 335, 1290
BclI TGATCA 2 cut(s) 2269, 3117
BcoDI GTCTC 8 cut(s) 27, 209, 215, 329, 1854, 1986, 2148, 2721
BcuI ACTAGT 2 cut(s) 770, 1262
BfaI CTAG 9 cut(s) 771, 815, 1046, 1149, 1185, 1263, 1383, 1790, 2724
BfmI CTRYAG 5 cut(s) 252, 2674, 3017, 3049, 3252
BfuAI ACCTGC 2 cut(s) 1314, 3178
BfuI GTATCC 2 cut(s) 335, 1290
BglII AGATCT 1 cut(s) 680
BlpI GCTNAGC 1 cut(s) 71
BmcAI AGTACT 3 cut(s) 1261, 2557, 2798
Bme1390I CCNGG 9 cut(s) 732, 1357, 1490, 1664, 2415, 2507, 2988, 3261, 3273
Bme18I GGWCC 3 cut(s) 129, 505, 2948
BmgBI CACGTC 1 cut(s) 1586
BmgT120I GGNCC 4 cut(s) 129, 505, 2090, 2948
BmiI GGNNCC 4 cut(s) 1450, 1555, 2107, 2220
BmrFI CCNGG 9 cut(s) 732, 1357, 1490, 1664, 2415, 2507, 2988, 3261, 3273
BmsI GCATC 6 cut(s) 577, 1472, 1628, 2341, 2686, 2829
BpiI GAAGAC 2 cut(s) 667, 2655
BplI GAGNNNNNCTC 2 cut(s) 2181, 2213
BpmI CTGGAG 5 cut(s) 375, 1917, 2124, 2970, 3243
Bpu10I CCTNAGC 1 cut(s) 2255
Bpu1102I GCTNAGC 1 cut(s) 71
BpuEI CTTGAG 1 cut(s) 2942
Bsa29I ATCGAT 1 cut(s) 2706
BsaBI GATNNNNATC 2 cut(s) 1278, 1371
BsaI GGTCTC 3 cut(s) 329, 1854, 1986
BsaJI CCNNGG 6 cut(s) 125, 1283, 1356, 2413, 2505, 2937
BsaWI WCCGGW 1 cut(s) 407
BsaXI ACNNNNNCTCC 6 cut(s) 349, 379, 1249, 1279, 1828, 1858
Bsc4I CCNNNNNNNGG 9 cut(s) 257, 1274, 1563, 1832, 1976, 2260, 2292, 2296, 2603
Bse118I RCCGGY 2 cut(s) 1726, 2603
Bse1I ACTGG 5 cut(s) 454, 723, 1561, 1900, 1970
Bse3DI GCAATG 4 cut(s) 337, 729, 1804, 2490
Bse8I GATNNNNATC 2 cut(s) 1278, 1371
BseBI CCWGG 9 cut(s) 732, 1357, 1490, 1664, 2415, 2507, 2988, 3261, 3273
BseCI ATCGAT 1 cut(s) 2706
BseDI CCNNGG 6 cut(s) 125, 1283, 1356, 2413, 2505, 2937
BseJI GATNNNNATC 2 cut(s) 1278, 1371
BseLI CCNNNNNNNGG 9 cut(s) 257, 1274, 1563, 1832, 1976, 2260, 2292, 2296, 2603
BseMI GCAATG 4 cut(s) 337, 729, 1804, 2490
BseMII CTCAG 5 cut(s) 1086, 1637, 2246, 2728, 2814
BseNI ACTGG 5 cut(s) 454, 723, 1561, 1900, 1970
BseRI GAGGAG 2 cut(s) 2573, 2620
BseSI GKGCMC 1 cut(s) 2487
BseXI GCAGC 8 cut(s) 541, 577, 946, 949, 1613, 3119, 3238, 3266
BsgI GTGCAG 3 cut(s) 948, 2985, 3118
Bsh1236I CGCG 1 cut(s) 2939
Bsh1285I CGRYCG 1 cut(s) 2290
BshFI GGCC 5 cut(s) 735, 884, 1488, 1902, 2092
BshVI ATCGAT 1 cut(s) 2706
BsiEI CGRYCG 1 cut(s) 2290
BsiHKAI GWGCWC 2 cut(s) 2253, 2487
BsiSI CCGG 4 cut(s) 408, 1727, 2604, 2693
BslFI GGGAC 3 cut(s) 582, 1382, 3022
BslI CCNNNNNNNGG 9 cut(s) 257, 1274, 1563, 1832, 1976, 2260, 2292, 2296, 2603
BsmAI GTCTC 8 cut(s) 27, 209, 215, 329, 1854, 1986, 2148, 2721
BsmBI CGTCTC 2 cut(s) 215, 2148
BsmFI GGGAC 3 cut(s) 582, 1382, 3022
BsmI GAATGC 1 cut(s) 1928
BsnI GGCC 5 cut(s) 735, 884, 1488, 1902, 2092
Bso31I GGTCTC 3 cut(s) 329, 1854, 1986
Bsp1286I GDGCHC 2 cut(s) 2253, 2487
Bsp1407I TGTACA 1 cut(s) 176
Bsp143I GATC 8 cut(s) 680, 1448, 1511, 2060, 2218, 2269, 2733, 3117
Bsp1720I GCTNAGC 1 cut(s) 71
Bsp19I CCATGG 1 cut(s) 1283
BspACI CCGC 8 cut(s) 75, 508, 1379, 2290, 2663, 2937, 2939, 3041
BspANI GGCC 5 cut(s) 735, 884, 1488, 1902, 2092
BspCNI CTCAG 5 cut(s) 1087, 1636, 2247, 2727, 2813
BspDI ATCGAT 1 cut(s) 2706
BspFNI CGCG 1 cut(s) 2939
BspLI GGNNCC 4 cut(s) 1450, 1555, 2107, 2220
BspMAI CTGCAG 4 cut(s) 2678, 3021, 3053, 3256
BspMI ACCTGC 2 cut(s) 1314, 3178
BspPI GGATC 6 cut(s) 1443, 1456, 1519, 2068, 2213, 2226
BspQI GCTCTTC 2 cut(s) 1047, 2130
BspTNI GGTCTC 3 cut(s) 329, 1854, 1986
BsrDI GCAATG 4 cut(s) 337, 729, 1804, 2490
BsrFI RCCGGY 2 cut(s) 1726, 2603
BsrGI TGTACA 1 cut(s) 176
BsrI ACTGG 5 cut(s) 454, 723, 1561, 1900, 1970
BssAI RCCGGY 2 cut(s) 1726, 2603
BssECI CCNNGG 6 cut(s) 125, 1283, 1356, 2413, 2505, 2937
BssMI GATC 8 cut(s) 680, 1448, 1511, 2060, 2218, 2269, 2733, 3117
BssNAI GTATAC 1 cut(s) 248
BssT1I CCWWGG 2 cut(s) 125, 1283
Bst1107I GTATAC 1 cut(s) 248
Bst2UI CCWGG 9 cut(s) 732, 1357, 1490, 1664, 2415, 2507, 2988, 3261, 3273
Bst4CI ACNGT 8 cut(s) 379, 1170, 1739, 2528, 2555, 2618, 2796, 2846
Bst6I CTCTTC 6 cut(s) 903, 1047, 2130, 2434, 2757, 2958
BstAPI GCANNNNNTGC 2 cut(s) 971, 3057
BstAUI TGTACA 1 cut(s) 176
BstC8I GCNNGC 5 cut(s) 2110, 2605, 2742, 2746, 3130
BstDEI CTNAG 9 cut(s) 47, 71, 1026, 1095, 1623, 2255, 2714, 2800, 2924
BstDSI CCRYGG 2 cut(s) 1283, 2937
BstFNI CGCG 1 cut(s) 2939
BstHHI GCGC 1 cut(s) 1789
BstKTI GATC 8 cut(s) 683, 1451, 1514, 2063, 2221, 2272, 2736, 3120
BstMAI GTCTC 8 cut(s) 27, 209, 215, 329, 1854, 1986, 2148, 2721
BstMBI GATC 8 cut(s) 680, 1448, 1511, 2060, 2218, 2269, 2733, 3117
BstMCI CGRYCG 1 cut(s) 2290
BstMWI GCNNNNNNNGC 5 cut(s) 526, 797, 971, 2322, 3057
BstNI CCWGG 9 cut(s) 732, 1357, 1490, 1664, 2415, 2507, 2988, 3261, 3273
BstNSI RCATGY 4 cut(s) 1126, 1314, 2039, 2748
BstSCI CCNGG 9 cut(s) 730, 1355, 1488, 1662, 2413, 2505, 2986, 3259, 3271
BstSFI CTRYAG 5 cut(s) 252, 2674, 3017, 3049, 3252
BstSLI GKGCMC 1 cut(s) 2487
BstUI CGCG 1 cut(s) 2939
BstV1I GCAGC 8 cut(s) 541, 577, 946, 949, 1613, 3119, 3238, 3266
BstV2I GAAGAC 2 cut(s) 667, 2655
BstX2I RGATCY 4 cut(s) 680, 1448, 2060, 2218
BstXI CCANNNNNNTGG 1 cut(s) 1706
BstYI RGATCY 4 cut(s) 680, 1448, 2060, 2218
BstZ17I GTATAC 1 cut(s) 248
Bsu15I ATCGAT 1 cut(s) 2706
BsuI GTATCC 2 cut(s) 335, 1290
BsuRI GGCC 5 cut(s) 735, 884, 1488, 1902, 2092
BsuTUI ATCGAT 1 cut(s) 2706
BtgI CCRYGG 2 cut(s) 1283, 2937
BtrI CACGTC 1 cut(s) 1586
BtsI GCAGTG 5 cut(s) 484, 2485, 2973, 3026, 3058
BtsIMutI CAGTG 9 cut(s) 53, 484, 716, 1568, 2355, 2485, 2973, 3026, 3058
BveI ACCTGC 2 cut(s) 1314, 3178
Cac8I GCNNGC 5 cut(s) 2110, 2605, 2742, 2746, 3130
CaiI CAGNNNCTG 1 cut(s) 398
CfoI GCGC 1 cut(s) 1789
Cfr10I RCCGGY 2 cut(s) 1726, 2603
Cfr13I GGNCC 4 cut(s) 129, 505, 2090, 2948
Cfr42I CCGCGG 1 cut(s) 2940
ClaI ATCGAT 1 cut(s) 2706
CseI GACGC 2 cut(s) 232, 1469
DdeI CTNAG 9 cut(s) 47, 71, 1026, 1095, 1623, 2255, 2714, 2800, 2924
DpnI GATC 8 cut(s) 682, 1450, 1513, 2062, 2220, 2271, 2735, 3119
DpnII GATC 8 cut(s) 680, 1448, 1511, 2060, 2218, 2269, 2733, 3117
DrdI GACNNNNNNGTC 2 cut(s) 429, 2928
DseDI GACNNNNNNGTC 2 cut(s) 429, 2928
EaeI YGGCCR 1 cut(s) 733
Eam1104I CTCTTC 6 cut(s) 903, 1047, 2130, 2434, 2757, 2958
EarI CTCTTC 6 cut(s) 903, 1047, 2130, 2434, 2757, 2958
Eco130I CCWWGG 2 cut(s) 125, 1283
Eco147I AGGCCT 1 cut(s) 884
Eco31I GGTCTC 3 cut(s) 329, 1854, 1986
Eco32I GATATC 1 cut(s) 1495
Eco47I GGWCC 3 cut(s) 129, 505, 2948
Eco57I CTGAAG 6 cut(s) 927, 1236, 2379, 2738, 3198, 3222
EcoO109I RGGNCCY 1 cut(s) 129
EcoRI GAATTC 1 cut(s) 1288
EcoRII CCWGG 9 cut(s) 730, 1355, 1488, 1662, 2413, 2505, 2986, 3259, 3271
EcoRV GATATC 1 cut(s) 1495
EcoT14I CCWWGG 2 cut(s) 125, 1283
ErhI CCWWGG 2 cut(s) 125, 1283
Esp3I CGTCTC 2 cut(s) 215, 2148
FalI AAGNNNNNCTT 6 cut(s) 606, 638, 822, 854, 1678, 1710
FaqI GGGAC 3 cut(s) 582, 1382, 3022
FauI CCCGC 1 cut(s) 3048
FauNDI CATATG 1 cut(s) 1768
FbaI TGATCA 2 cut(s) 2269, 3117
FblI GTMKAC 1 cut(s) 247
FspBI CTAG 9 cut(s) 771, 815, 1046, 1149, 1185, 1263, 1383, 1790, 2724
GlaI GCGC 1 cut(s) 1788
GsuI CTGGAG 5 cut(s) 375, 1917, 2124, 2970, 3243
HaeIII GGCC 5 cut(s) 735, 884, 1488, 1902, 2092
HapII CCGG 4 cut(s) 408, 1727, 2604, 2693
HgaI GACGC 2 cut(s) 232, 1469
HhaI GCGC 1 cut(s) 1789
Hin6I GCGC 1 cut(s) 1787
HinP1I GCGC 1 cut(s) 1787
HincII GTYRAC 4 cut(s) 706, 784, 1441, 1459
HindII GTYRAC 4 cut(s) 706, 784, 1441, 1459
HindIII AAGCTT 1 cut(s) 1028
HpaII CCGG 4 cut(s) 408, 1727, 2604, 2693
HphI GGTGA 7 cut(s) 182, 464, 1372, 1458, 1625, 1721, 3127
Hpy166II GTNNAC 9 cut(s) 53, 248, 706, 784, 1441, 1459, 2195, 2485, 3244
Hpy8I GTNNAC 9 cut(s) 53, 248, 706, 784, 1441, 1459, 2195, 2485, 3244
HpyAV CCTTC 9 cut(s) 23, 296, 492, 496, 1182, 1574, 2354, 2847, 3022
HpyCH4III ACNGT 8 cut(s) 379, 1170, 1739, 2528, 2555, 2618, 2796, 2846
HpyCH4IV ACGT 3 cut(s) 1585, 1703, 2865
HpyF10VI GCNNNNNNNGC 5 cut(s) 526, 797, 971, 2322, 3057
HpyF3I CTNAG 9 cut(s) 47, 71, 1026, 1095, 1623, 2255, 2714, 2800, 2924
HpySE526I ACGT 3 cut(s) 1585, 1703, 2865
HspAI GCGC 1 cut(s) 1787
KroI GCCGGC 1 cut(s) 2603
KroNI GCCGGC 1 cut(s) 2605
Ksp22I TGATCA 2 cut(s) 2269, 3117
KspI CCGCGG 1 cut(s) 2940
Kzo9I GATC 8 cut(s) 680, 1448, 1511, 2060, 2218, 2269, 2733, 3117
LguI GCTCTTC 2 cut(s) 1047, 2130
LmnI GCTCC 2 cut(s) 2105, 3262
Lsp1109I GCAGC 8 cut(s) 541, 577, 946, 949, 1613, 3119, 3238, 3266
LweI GCATC 6 cut(s) 577, 1472, 1628, 2341, 2686, 2829
MaeI CTAG 9 cut(s) 771, 815, 1046, 1149, 1185, 1263, 1383, 1790, 2724
MaeII ACGT 3 cut(s) 1585, 1703, 2865
MaeIII GTNAC 7 cut(s) 479, 580, 1569, 1613, 1679, 1719, 2537
MalI GATC 8 cut(s) 682, 1450, 1513, 2062, 2220, 2271, 2735, 3119
MboI GATC 8 cut(s) 680, 1448, 1511, 2060, 2218, 2269, 2733, 3117
MfeI CAATTG 3 cut(s) 240, 1748, 2782
MflI RGATCY 4 cut(s) 680, 1448, 2060, 2218
MhlI GDGCHC 2 cut(s) 2253, 2487
MlsI TGGCCA 1 cut(s) 735
MluNI TGGCCA 1 cut(s) 735
MmeI TCCRAC 4 cut(s) 677, 1615, 1670, 2385
Mox20I TGGCCA 1 cut(s) 735
MroNI GCCGGC 1 cut(s) 2603
MroXI GAANNNNTTC 2 cut(s) 756, 1552
MscI TGGCCA 1 cut(s) 735
MslI CAYNNNNRTG 3 cut(s) 477, 576, 1809
Msp20I TGGCCA 1 cut(s) 735
MspA1I CMGCKG 2 cut(s) 2939, 3132
MspI CCGG 4 cut(s) 408, 1727, 2604, 2693
MspR9I CCNGG 9 cut(s) 732, 1357, 1490, 1664, 2415, 2507, 2988, 3261, 3273
MunI CAATTG 3 cut(s) 240, 1748, 2782
Mva1269I GAATGC 1 cut(s) 1928
MvaI CCWGG 9 cut(s) 732, 1357, 1490, 1664, 2415, 2507, 2988, 3261, 3273
MvnI CGCG 1 cut(s) 2939
MwoI GCNNNNNNNGC 5 cut(s) 526, 797, 971, 2322, 3057
NaeI GCCGGC 1 cut(s) 2605
NcoI CCATGG 1 cut(s) 1283
NdeI CATATG 1 cut(s) 1768
NdeII GATC 8 cut(s) 680, 1448, 1511, 2060, 2218, 2269, 2733, 3117
NgoMIV GCCGGC 1 cut(s) 2603
NlaIV GGNNCC 4 cut(s) 1450, 1555, 2107, 2220
NmeAIII GCCGAG 1 cut(s) 2616
NmuCI GTSAC 3 cut(s) 479, 1613, 1719
NspI RCATGY 4 cut(s) 1126, 1314, 2039, 2748
PaeI GCATGC 1 cut(s) 2748
PaqCI CACCTGC 1 cut(s) 1314
PceI AGGCCT 1 cut(s) 884
PciI ACATGT 2 cut(s) 1122, 1310
PciSI GCTCTTC 2 cut(s) 1047, 2130
PcsI WCGNNNNNNNCGW 2 cut(s) 1931, 2862
PctI GAATGC 1 cut(s) 1928
PdiI GCCGGC 1 cut(s) 2605
PdmI GAANNNNTTC 2 cut(s) 756, 1552
PflFI GACNNNGTC 1 cut(s) 2654
PflMI CCANNNNNTGG 4 cut(s) 1274, 1563, 1832, 1976
PfoI TCCNGGA 3 cut(s) 1662, 3259, 3271
PpuMI RGGWCCY 1 cut(s) 129
PscI ACATGT 2 cut(s) 1122, 1310
PsiI TTATAA 3 cut(s) 644, 746, 1797
Psp5II RGGWCCY 1 cut(s) 129
Psp6I CCWGG 9 cut(s) 730, 1355, 1488, 1662, 2413, 2505, 2986, 3259, 3271
PspGI CCWGG 9 cut(s) 730, 1355, 1488, 1662, 2413, 2505, 2986, 3259, 3271
PspN4I GGNNCC 4 cut(s) 1450, 1555, 2107, 2220
PspPI GGNCC 4 cut(s) 129, 505, 2090, 2948
PspPPI RGGWCCY 1 cut(s) 129
PsrI GAACNNNNNNTAC 2 cut(s) 1297, 1329
PstI CTGCAG 4 cut(s) 2678, 3021, 3053, 3256
PstNI CAGNNNCTG 1 cut(s) 398
PsuI RGATCY 4 cut(s) 680, 1448, 2060, 2218
PsyI GACNNNGTC 1 cut(s) 2654
PvuII CAGCTG 1 cut(s) 3132
RseI CAYNNNNRTG 3 cut(s) 477, 576, 1809
SacII CCGCGG 1 cut(s) 2940
SapI GCTCTTC 2 cut(s) 1047, 2130
Sau3AI GATC 8 cut(s) 680, 1448, 1511, 2060, 2218, 2269, 2733, 3117
Sau96I GGNCC 4 cut(s) 129, 505, 2090, 2948
ScaI AGTACT 3 cut(s) 1261, 2557, 2798
ScrFI CCNGG 9 cut(s) 732, 1357, 1490, 1664, 2415, 2507, 2988, 3261, 3273
SduI GDGCHC 2 cut(s) 2253, 2487
SfaNI GCATC 6 cut(s) 577, 1472, 1628, 2341, 2686, 2829
SfcI CTRYAG 5 cut(s) 252, 2674, 3017, 3049, 3252
Sfr303I CCGCGG 1 cut(s) 2940
SgrBI CCGCGG 1 cut(s) 2940
SinI GGWCC 3 cut(s) 129, 505, 2948
SmiMI CAYNNNNRTG 3 cut(s) 477, 576, 1809
SmlI CTYRAG 1 cut(s) 2957
SmoI CTYRAG 1 cut(s) 2957
SpeI ACTAGT 2 cut(s) 770, 1262
SphI GCATGC 1 cut(s) 2748
SseBI AGGCCT 1 cut(s) 884
SsiI CCGC 8 cut(s) 75, 508, 1379, 2290, 2663, 2937, 2939, 3041
SspI AATATT 2 cut(s) 675, 2011
SspMI CTAG 9 cut(s) 771, 815, 1046, 1149, 1185, 1263, 1383, 1790, 2724
StuI AGGCCT 1 cut(s) 884
StyD4I CCNGG 9 cut(s) 730, 1355, 1488, 1662, 2413, 2505, 2986, 3259, 3271
StyI CCWWGG 2 cut(s) 125, 1283
TaaI ACNGT 8 cut(s) 379, 1170, 1739, 2528, 2555, 2618, 2796, 2846
TaiI ACGT 3 cut(s) 1588, 1706, 2868
TaqI TCGA 9 cut(s) 31, 1408, 1578, 2175, 2181, 2244, 2706, 3078, 3180
TatI WGTACW 6 cut(s) 176, 292, 1259, 1876, 2555, 2796
TauI GCSGC 4 cut(s) 78, 1382, 2939, 2942
TscAI CASTG 9 cut(s) 60, 484, 723, 1568, 2362, 2492, 2973, 3026, 3058
TseFI GTSAC 3 cut(s) 479, 1613, 1719
TseI GCWGC 8 cut(s) 529, 590, 959, 962, 1601, 3132, 3251, 3254
Tsp45I GTSAC 3 cut(s) 479, 1613, 1719
TspGWI ACGGA 2 cut(s) 173, 2172
TspRI CASTG 9 cut(s) 60, 484, 723, 1568, 2362, 2492, 2973, 3026, 3058
Tth111I GACNNNGTC 1 cut(s) 2654
Van91I CCANNNNNTGG 4 cut(s) 1274, 1563, 1832, 1976
VneI GTGCAC 1 cut(s) 2483
VpaK11BI GGWCC 3 cut(s) 129, 505, 2948
XapI RAATTY 8 cut(s) 58, 81, 309, 949, 1288, 2377, 2454, 2974
XbaI TCTAGA 1 cut(s) 1045
XceI RCATGY 4 cut(s) 1126, 1314, 2039, 2748
XcmI CCANNNNNNNNNTGG 2 cut(s) 836, 3145
XmiI GTMKAC 1 cut(s) 247
XmnI GAANNNNTTC 2 cut(s) 756, 1552
XspI CTAG 9 cut(s) 771, 815, 1046, 1149, 1185, 1263, 1383, 1790, 2724
ZrmI AGTACT 3 cut(s) 1261, 2557, 2798
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.