acyl-activating enzyme
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1008 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 674 |
| Acc36I | ACCTGC | 3 cut(s) | 740, 1008, 3076 |
| Acc65I | GGTACC | 1 cut(s) | 3027 |
| AccB1I | GGYRCC | 2 cut(s) | 326, 3027 |
| AccB7I | CCANNNNNTGG | 5 cut(s) | 950, 2385, 2432, 2789, 2933 |
| AccBSI | CCGCTC | 2 cut(s) | 386, 1183 |
| AccII | CGCG | 1 cut(s) | 264 |
| AclWI | GGATC | 9 cut(s) | 103, 109, 116, 2215, 2728, 3019, 3164, 3177, 3215 |
| AcoI | YGGCCR | 3 cut(s) | 657, 1882, 2857 |
| AcsI | RAATTY | 6 cut(s) | 1007, 1285, 1458, 2446, 3338, 3399 |
| AcuI | CTGAAG | 1 cut(s) | 2394 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 99, 633, 950, 1452, 2385, 2432, 2789, 2933 |
| AflII | CTTAAG | 1 cut(s) | 1064 |
| AflIII | ACRYGT | 2 cut(s) | 2461, 2629 |
| AgeI | ACCGGT | 1 cut(s) | 915 |
| AhlI | ACTAGT | 1 cut(s) | 3005 |
| AjiI | CACGTC | 1 cut(s) | 2989 |
| AjnI | CCWGG | 3 cut(s) | 620, 1268, 1879 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1138, 1170 |
| Alw21I | GWGCWC | 3 cut(s) | 1215, 3204, 3432 |
| Alw26I | GTCTC | 8 cut(s) | 722, 1358, 2288, 2695, 2816, 2943, 3099, 3357 |
| Alw44I | GTGCAC | 1 cut(s) | 3428 |
| AlwI | GGATC | 9 cut(s) | 103, 109, 116, 2215, 2728, 3019, 3164, 3177, 3215 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 937 |
| Ama87I | CYCGRG | 1 cut(s) | 3295 |
| AoxI | GGCC | 7 cut(s) | 517, 623, 657, 1882, 2031, 2857, 3041 |
| ApaLI | GTGCAC | 1 cut(s) | 3428 |
| ApeKI | GCWGC | 9 cut(s) | 252, 278, 377, 1678, 1739, 2108, 2111, 2645, 3302 |
| ApoI | RAATTY | 6 cut(s) | 1007, 1285, 1458, 2446, 3338, 3399 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 291, 323 |
| AsiGI | ACCGGT | 1 cut(s) | 915 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 917, 1905, 2344 |
| Asp718I | GGTACC | 1 cut(s) | 3027 |
| AspLEI | GCGC | 1 cut(s) | 1019 |
| AspS9I | GGNCC | 8 cut(s) | 518, 613, 624, 1272, 2031, 2423, 3041, 3352 |
| AsuC2I | CCSGG | 2 cut(s) | 871, 3287 |
| AsuII | TTCGAA | 1 cut(s) | 702 |
| AvaI | CYCGRG | 1 cut(s) | 3295 |
| AvaII | GGWCC | 4 cut(s) | 613, 1272, 2423, 3352 |
| BaeGI | GKGCMC | 2 cut(s) | 331, 3432 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 1564, 1597, 2856, 2889 |
| BalI | TGGCCA | 1 cut(s) | 1884 |
| BamHI | GGATCC | 2 cut(s) | 108, 3169 |
| BanI | GGYRCC | 2 cut(s) | 326, 3027 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 1521, 1553, 2763, 2795 |
| BbsI | GAAGAC | 1 cut(s) | 1908 |
| Bbv12I | GWGCWC | 3 cut(s) | 1215, 3204, 3432 |
| BbvCI | CCTCAGC | 1 cut(s) | 448 |
| BbvI | GCAGC | 9 cut(s) | 239, 290, 364, 1690, 1726, 2095, 2098, 2657, 3289 |
| BceAI | ACGGC | 5 cut(s) | 290, 291, 430, 437, 532 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 359, 393 |
| BciT130I | CCWGG | 3 cut(s) | 622, 1270, 1881 |
| BciVI | GTATCC | 6 cut(s) | 951, 1453, 1484, 1565, 2448, 2995 |
| BclI | TGATCA | 1 cut(s) | 994 |
| BcnI | CCSGG | 2 cut(s) | 871, 3287 |
| BcoDI | GTCTC | 8 cut(s) | 722, 1358, 2288, 2695, 2816, 2943, 3099, 3357 |
| BcuI | ACTAGT | 1 cut(s) | 3005 |
| BfmI | CTRYAG | 1 cut(s) | 1001 |
| BfrI | CTTAAG | 1 cut(s) | 1064 |
| BfuAI | ACCTGC | 3 cut(s) | 740, 1008, 3076 |
| BfuI | GTATCC | 6 cut(s) | 951, 1453, 1484, 1565, 2448, 2995 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 77 |
| BlpI | GCTNAGC | 1 cut(s) | 1214 |
| BmcAI | AGTACT | 1 cut(s) | 1300 |
| Bme1390I | CCNGG | 5 cut(s) | 622, 871, 1270, 1881, 3287 |
| Bme18I | GGWCC | 4 cut(s) | 613, 1272, 2423, 3352 |
| BmeT110I | CYCGRG | 1 cut(s) | 3295 |
| BmgBI | CACGTC | 1 cut(s) | 2989 |
| BmgT120I | GGNCC | 8 cut(s) | 518, 613, 624, 1272, 2031, 2423, 3041, 3352 |
| BmiI | GGNNCC | 8 cut(s) | 110, 328, 615, 920, 2003, 3029, 3058, 3171 |
| BmrFI | CCNGG | 5 cut(s) | 622, 871, 1270, 1881, 3287 |
| BmrI | ACTGGG | 1 cut(s) | 1809 |
| BmsI | GCATC | 9 cut(s) | 189, 202, 364, 578, 918, 1239, 2356, 2805, 3123 |
| BmuI | ACTGGG | 1 cut(s) | 1809 |
| BpiI | GAAGAC | 1 cut(s) | 1908 |
| BpmI | CTGGAG | 4 cut(s) | 173, 2910, 3075, 3159 |
| Bpu10I | CCTNAGC | 2 cut(s) | 448, 3265 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1214 |
| Bpu14I | TTCGAA | 1 cut(s) | 702 |
| BpuEI | CTTGAG | 1 cut(s) | 3226 |
| BpuMI | CCSGG | 2 cut(s) | 871, 3287 |
| Bsa29I | ATCGAT | 1 cut(s) | 795 |
| BsaAI | YACGTR | 1 cut(s) | 432 |
| BsaBI | GATNNNNATC | 3 cut(s) | 113, 703, 2436 |
| BsaI | GGTCTC | 3 cut(s) | 722, 2816, 2943 |
| BsaJI | CCNNGG | 6 cut(s) | 273, 654, 1268, 2441, 2997, 3355 |
| BsaWI | WCCGGW | 6 cut(s) | 320, 724, 860, 915, 921, 977 |
| BsaXI | ACNNNNNCTCC | 7 cut(s) | 29, 387, 417, 2785, 2815, 3351, 3381 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 99, 633, 950, 1452, 2385, 2432, 2789, 2933 |
| Bse118I | RCCGGY | 2 cut(s) | 915, 2481 |
| Bse1I | ACTGG | 9 cut(s) | 81, 1033, 1446, 1603, 1815, 2425, 2927, 3135, 3142 |
| Bse3DI | GCAATG | 2 cut(s) | 247, 2761 |
| Bse8I | GATNNNNATC | 3 cut(s) | 113, 703, 2436 |
| BseBI | CCWGG | 3 cut(s) | 622, 1270, 1881 |
| BseCI | ATCGAT | 1 cut(s) | 795 |
| BseDI | CCNNGG | 6 cut(s) | 273, 654, 1268, 2441, 2997, 3355 |
| BseGI | GGATG | 6 cut(s) | 933, 1720, 1854, 3202, 3334, 3424 |
| BseJI | GATNNNNATC | 3 cut(s) | 113, 703, 2436 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 99, 633, 950, 1452, 2385, 2432, 2789, 2933 |
| BseMI | GCAATG | 2 cut(s) | 247, 2761 |
| BseMII | CTCAG | 7 cut(s) | 462, 888, 1027, 1228, 1293, 2283, 3279 |
| BseNI | ACTGG | 9 cut(s) | 81, 1033, 1446, 1603, 1815, 2425, 2927, 3135, 3142 |
| BseSI | GKGCMC | 2 cut(s) | 331, 3432 |
| BseX3I | CGGCCG | 1 cut(s) | 657 |
| BseXI | GCAGC | 9 cut(s) | 239, 290, 364, 1690, 1726, 2095, 2098, 2657, 3289 |
| BseYI | CCCAGC | 3 cut(s) | 471, 497, 3038 |
| BsgI | GTGCAG | 3 cut(s) | 166, 2624, 2995 |
| Bsh1236I | CGCG | 1 cut(s) | 264 |
| Bsh1285I | CGRYCG | 2 cut(s) | 660, 870 |
| BshFI | GGCC | 7 cut(s) | 519, 625, 659, 1884, 2033, 2859, 3043 |
| BshNI | GGYRCC | 2 cut(s) | 326, 3027 |
| BshTI | ACCGGT | 1 cut(s) | 915 |
| BshVI | ATCGAT | 1 cut(s) | 795 |
| BsiEI | CGRYCG | 2 cut(s) | 660, 870 |
| BsiHKAI | GWGCWC | 3 cut(s) | 1215, 3204, 3432 |
| BsiHKCI | CYCGRG | 1 cut(s) | 3295 |
| BslFI | GGGAC | 3 cut(s) | 336, 918, 1731 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 99, 633, 950, 1452, 2385, 2432, 2789, 2933 |
| BsmAI | GTCTC | 8 cut(s) | 722, 1358, 2288, 2695, 2816, 2943, 3099, 3357 |
| BsmBI | CGTCTC | 3 cut(s) | 1358, 3099, 3357 |
| BsmFI | GGGAC | 3 cut(s) | 336, 918, 1731 |
| BsnI | GGCC | 7 cut(s) | 519, 625, 659, 1884, 2033, 2859, 3043 |
| Bso31I | GGTCTC | 3 cut(s) | 722, 2816, 2943 |
| BsoBI | CYCGRG | 1 cut(s) | 3295 |
| Bsp119I | TTCGAA | 1 cut(s) | 702 |
| Bsp1286I | GDGCHC | 4 cut(s) | 331, 1215, 3204, 3432 |
| Bsp1407I | TGTACA | 2 cut(s) | 852, 1319 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1214 |
| Bsp19I | CCATGG | 1 cut(s) | 2441 |
| BspANI | GGCC | 7 cut(s) | 519, 625, 659, 1884, 2033, 2859, 3043 |
| BspCNI | CTCAG | 7 cut(s) | 461, 889, 1026, 1227, 1294, 2284, 3278 |
| BspDI | ATCGAT | 1 cut(s) | 795 |
| BspFNI | CGCG | 1 cut(s) | 264 |
| BspLI | GGNNCC | 8 cut(s) | 110, 328, 615, 920, 2003, 3029, 3058, 3171 |
| BspMAI | CTGCAG | 1 cut(s) | 1005 |
| BspMI | ACCTGC | 3 cut(s) | 740, 1008, 3076 |
| BspPI | GGATC | 9 cut(s) | 103, 109, 116, 2215, 2728, 3019, 3164, 3177, 3215 |
| BspQI | GCTCTTC | 1 cut(s) | 2196 |
| BspT104I | TTCGAA | 1 cut(s) | 702 |
| BspT107I | GGYRCC | 2 cut(s) | 326, 3027 |
| BspTI | CTTAAG | 1 cut(s) | 1064 |
| BspTNI | GGTCTC | 3 cut(s) | 722, 2816, 2943 |
| BsrBI | CCGCTC | 2 cut(s) | 386, 1183 |
| BsrDI | GCAATG | 2 cut(s) | 247, 2761 |
| BsrFI | RCCGGY | 2 cut(s) | 915, 2481 |
| BsrGI | TGTACA | 2 cut(s) | 852, 1319 |
| BsrI | ACTGG | 9 cut(s) | 81, 1033, 1446, 1603, 1815, 2425, 2927, 3135, 3142 |
| BssAI | RCCGGY | 2 cut(s) | 915, 2481 |
| BssECI | CCNNGG | 6 cut(s) | 273, 654, 1268, 2441, 2997, 3355 |
| BssT1I | CCWWGG | 2 cut(s) | 2441, 2997 |
| Bst2UI | CCWGG | 3 cut(s) | 622, 1270, 1881 |
| Bst4CI | ACNGT | 7 cut(s) | 576, 948, 1528, 1658, 2522, 2696, 3442 |
| Bst6I | CTCTTC | 3 cut(s) | 759, 2196, 3379 |
| BstAFI | CTTAAG | 1 cut(s) | 1064 |
| BstAUI | TGTACA | 2 cut(s) | 852, 1319 |
| BstBAI | YACGTR | 1 cut(s) | 432 |
| BstBI | TTCGAA | 1 cut(s) | 702 |
| BstC8I | GCNNGC | 4 cut(s) | 805, 1397, 2994, 3061 |
| BstDEI | CTNAG | 9 cut(s) | 448, 897, 1013, 1214, 1302, 2175, 2292, 2336, 3265 |
| BstDSI | CCRYGG | 2 cut(s) | 273, 2441 |
| BstEII | GGTNACC | 1 cut(s) | 2805 |
| BstF5I | GGATG | 6 cut(s) | 933, 1720, 1854, 3202, 3334, 3424 |
| BstFNI | CGCG | 1 cut(s) | 264 |
| BstHHI | GCGC | 1 cut(s) | 1019 |
| BstMAI | GTCTC | 8 cut(s) | 722, 1358, 2288, 2695, 2816, 2943, 3099, 3357 |
| BstMCI | CGRYCG | 2 cut(s) | 660, 870 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 62, 77, 199, 261, 383, 2414, 3232, 3270, 3311 |
| BstNI | CCWGG | 3 cut(s) | 622, 1270, 1881 |
| BstNSI | RCATGY | 4 cut(s) | 544, 1399, 2465, 2633 |
| BstPI | GGTNACC | 1 cut(s) | 2805 |
| BstSCI | CCNGG | 5 cut(s) | 620, 869, 1268, 1879, 3285 |
| BstSFI | CTRYAG | 1 cut(s) | 1001 |
| BstSLI | GKGCMC | 2 cut(s) | 331, 3432 |
| BstUI | CGCG | 1 cut(s) | 264 |
| BstV1I | GCAGC | 9 cut(s) | 239, 290, 364, 1690, 1726, 2095, 2098, 2657, 3289 |
| BstV2I | GAAGAC | 1 cut(s) | 1908 |
| BstX2I | RGATCY | 3 cut(s) | 108, 3011, 3169 |
| BstYI | RGATCY | 3 cut(s) | 108, 3011, 3169 |
| BstZI | CGGCCG | 1 cut(s) | 657 |
| Bsu15I | ATCGAT | 1 cut(s) | 795 |
| BsuI | GTATCC | 6 cut(s) | 951, 1453, 1484, 1565, 2448, 2995 |
| BsuRI | GGCC | 7 cut(s) | 519, 625, 659, 1884, 2033, 2859, 3043 |
| BsuTUI | ATCGAT | 1 cut(s) | 795 |
| BtgI | CCRYGG | 2 cut(s) | 273, 2441 |
| BtgZI | GCGATG | 1 cut(s) | 601 |
| BtrI | CACGTC | 1 cut(s) | 2989 |
| BtsCI | GGATG | 6 cut(s) | 933, 1720, 1854, 3202, 3334, 3424 |
| BtsI | GCAGTG | 3 cut(s) | 748, 2612, 3430 |
| BtsIMutI | CAGTG | 8 cut(s) | 748, 944, 1453, 1822, 1903, 2612, 2934, 3430 |
| BveI | ACCTGC | 3 cut(s) | 740, 1008, 3076 |
| Cac8I | GCNNGC | 4 cut(s) | 805, 1397, 2994, 3061 |
| CaiI | CAGNNNCTG | 1 cut(s) | 937 |
| CfoI | GCGC | 1 cut(s) | 1019 |
| Cfr10I | RCCGGY | 2 cut(s) | 915, 2481 |
| Cfr13I | GGNCC | 8 cut(s) | 518, 613, 624, 1272, 2031, 2423, 3041, 3352 |
| ClaI | ATCGAT | 1 cut(s) | 795 |
| CseI | GACGC | 2 cut(s) | 697, 1745 |
| CspAI | ACCGGT | 1 cut(s) | 915 |
| DdeI | CTNAG | 9 cut(s) | 448, 897, 1013, 1214, 1302, 2175, 2292, 2336, 3265 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 674 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 674 |
| EaeI | YGGCCR | 3 cut(s) | 657, 1882, 2857 |
| EagI | CGGCCG | 1 cut(s) | 657 |
| Eam1104I | CTCTTC | 3 cut(s) | 759, 2196, 3379 |
| EarI | CTCTTC | 3 cut(s) | 759, 2196, 3379 |
| EciI | GGCGGA | 3 cut(s) | 472, 569, 3213 |
| EclXI | CGGCCG | 1 cut(s) | 657 |
| Eco130I | CCWWGG | 2 cut(s) | 2441, 2997 |
| Eco31I | GGTCTC | 3 cut(s) | 722, 2816, 2943 |
| Eco47I | GGWCC | 4 cut(s) | 613, 1272, 2423, 3352 |
| Eco52I | CGGCCG | 1 cut(s) | 657 |
| Eco57I | CTGAAG | 1 cut(s) | 2394 |
| Eco88I | CYCGRG | 1 cut(s) | 3295 |
| Eco91I | GGTNACC | 1 cut(s) | 2805 |
| EcoO109I | RGGNCCY | 2 cut(s) | 1272, 2031 |
| EcoO65I | GGTNACC | 1 cut(s) | 2805 |
| EcoRI | GAATTC | 1 cut(s) | 2446 |
| EcoRII | CCWGG | 3 cut(s) | 620, 1268, 1879 |
| EcoT14I | CCWWGG | 2 cut(s) | 2441, 2997 |
| ErhI | CCWWGG | 2 cut(s) | 2441, 2997 |
| Esp3I | CGTCTC | 3 cut(s) | 1358, 3099, 3357 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1570, 1602 |
| FaqI | GGGAC | 3 cut(s) | 336, 918, 1731 |
| FauI | CCCGC | 5 cut(s) | 269, 347, 362, 391, 446 |
| FauNDI | CATATG | 1 cut(s) | 2725 |
| FbaI | TGATCA | 1 cut(s) | 994 |
| FokI | GGATG | 6 cut(s) | 940, 1707, 1861, 3209, 3341, 3431 |
| GlaI | GCGC | 1 cut(s) | 1018 |
| GsaI | CCCAGC | 3 cut(s) | 475, 501, 3042 |
| GsuI | CTGGAG | 4 cut(s) | 173, 2910, 3075, 3159 |
| HaeIII | GGCC | 7 cut(s) | 519, 625, 659, 1884, 2033, 2859, 3043 |
| HgaI | GACGC | 2 cut(s) | 697, 1745 |
| HhaI | GCGC | 1 cut(s) | 1019 |
| Hin6I | GCGC | 1 cut(s) | 1017 |
| HinP1I | GCGC | 1 cut(s) | 1017 |
| HincII | GTYRAC | 3 cut(s) | 667, 2317, 3292 |
| HindII | GTYRAC | 3 cut(s) | 667, 2317, 3292 |
| HindIII | AAGCTT | 2 cut(s) | 985, 2177 |
| HpaI | GTTAAC | 1 cut(s) | 3292 |
| Hpy166II | GTNNAC | 7 cut(s) | 667, 855, 1531, 2287, 2317, 3292, 3430 |
| Hpy8I | GTNNAC | 7 cut(s) | 667, 855, 1531, 2287, 2317, 3292, 3430 |
| Hpy99I | CGWCG | 5 cut(s) | 374, 416, 553, 665, 680 |
| HpyAV | CCTTC | 8 cut(s) | 141, 436, 627, 1163, 1167, 2170, 2252, 2618 |
| HpyCH4III | ACNGT | 7 cut(s) | 576, 948, 1528, 1658, 2522, 2696, 3442 |
| HpyCH4IV | ACGT | 5 cut(s) | 431, 663, 1533, 2988, 3366 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 62, 77, 199, 261, 383, 2414, 3232, 3270, 3311 |
| HpyF3I | CTNAG | 9 cut(s) | 448, 897, 1013, 1214, 1302, 2175, 2292, 2336, 3265 |
| HpySE526I | ACGT | 5 cut(s) | 431, 663, 1533, 2988, 3366 |
| HspAI | GCGC | 1 cut(s) | 1017 |
| KpnI | GGTACC | 1 cut(s) | 3031 |
| Ksp22I | TGATCA | 1 cut(s) | 994 |
| KspAI | GTTAAC | 1 cut(s) | 3292 |
| LguI | GCTCTTC | 1 cut(s) | 2196 |
| Lsp1109I | GCAGC | 9 cut(s) | 239, 290, 364, 1690, 1726, 2095, 2098, 2657, 3289 |
| LweI | GCATC | 9 cut(s) | 189, 202, 364, 578, 918, 1239, 2356, 2805, 3123 |
| MaeII | ACGT | 5 cut(s) | 431, 663, 1533, 2988, 3366 |
| MaeIII | GTNAC | 7 cut(s) | 503, 636, 1628, 1776, 2576, 2676, 2805 |
| MbiI | CCGCTC | 2 cut(s) | 386, 1183 |
| MfeI | CAATTG | 3 cut(s) | 1389, 2103, 2705 |
| MflI | RGATCY | 3 cut(s) | 108, 3011, 3169 |
| MhlI | GDGCHC | 4 cut(s) | 331, 1215, 3204, 3432 |
| MlsI | TGGCCA | 1 cut(s) | 1884 |
| MluNI | TGGCCA | 1 cut(s) | 1884 |
| MlyI | GAGTC | 2 cut(s) | 1496, 2290 |
| MmeI | TCCRAC | 4 cut(s) | 393, 418, 1214, 1826 |
| Mox20I | TGGCCA | 1 cut(s) | 1884 |
| MroXI | GAANNNNTTC | 3 cut(s) | 917, 1905, 2344 |
| MscI | TGGCCA | 1 cut(s) | 1884 |
| MslI | CAYNNNNRTG | 3 cut(s) | 539, 1725, 2766 |
| Msp20I | TGGCCA | 1 cut(s) | 1884 |
| MspA1I | CMGCKG | 2 cut(s) | 1218, 2870 |
| MspCI | CTTAAG | 1 cut(s) | 1064 |
| MspR9I | CCNGG | 5 cut(s) | 622, 871, 1270, 1881, 3287 |
| MunI | CAATTG | 3 cut(s) | 1389, 2103, 2705 |
| MvaI | CCWGG | 3 cut(s) | 622, 1270, 1881 |
| MvnI | CGCG | 1 cut(s) | 264 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 62, 77, 199, 261, 383, 2414, 3232, 3270, 3311 |
| NciI | CCSGG | 2 cut(s) | 871, 3287 |
| NcoI | CCATGG | 1 cut(s) | 2441 |
| NdeI | CATATG | 1 cut(s) | 2725 |
| NlaIV | GGNNCC | 8 cut(s) | 110, 328, 615, 920, 2003, 3029, 3058, 3171 |
| NmeAIII | GCCGAG | 3 cut(s) | 228, 495, 635 |
| NmuCI | GTSAC | 4 cut(s) | 1628, 1776, 2676, 2805 |
| NspI | RCATGY | 4 cut(s) | 544, 1399, 2465, 2633 |
| NspV | TTCGAA | 1 cut(s) | 702 |
| PaeI | GCATGC | 1 cut(s) | 1399 |
| PaqCI | CACCTGC | 1 cut(s) | 1008 |
| PciI | ACATGT | 2 cut(s) | 2461, 2629 |
| PciSI | GCTCTTC | 1 cut(s) | 2196 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 3363 |
| PdmI | GAANNNNTTC | 3 cut(s) | 917, 1905, 2344 |
| PflMI | CCANNNNNTGG | 5 cut(s) | 950, 2385, 2432, 2789, 2933 |
| PinAI | ACCGGT | 1 cut(s) | 915 |
| PleI | GAGTC | 2 cut(s) | 1496, 2290 |
| PpsI | GAGTC | 2 cut(s) | 1496, 2290 |
| Ppu21I | YACGTR | 1 cut(s) | 432 |
| PpuMI | RGGWCCY | 1 cut(s) | 1272 |
| PscI | ACATGT | 2 cut(s) | 2461, 2629 |
| Psp5II | RGGWCCY | 1 cut(s) | 1272 |
| Psp6I | CCWGG | 3 cut(s) | 620, 1268, 1879 |
| PspEI | GGTNACC | 1 cut(s) | 2805 |
| PspFI | CCCAGC | 3 cut(s) | 471, 497, 3038 |
| PspGI | CCWGG | 3 cut(s) | 620, 1268, 1879 |
| PspN4I | GGNNCC | 8 cut(s) | 110, 328, 615, 920, 2003, 3029, 3058, 3171 |
| PspPI | GGNCC | 8 cut(s) | 518, 613, 624, 1272, 2031, 2423, 3041, 3352 |
| PspPPI | RGGWCCY | 1 cut(s) | 1272 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 2962, 2994 |
| PstI | CTGCAG | 1 cut(s) | 1005 |
| PstNI | CAGNNNCTG | 1 cut(s) | 937 |
| PsuI | RGATCY | 3 cut(s) | 108, 3011, 3169 |
| PvuII | CAGCTG | 1 cut(s) | 2870 |
| RseI | CAYNNNNRTG | 3 cut(s) | 539, 1725, 2766 |
| SapI | GCTCTTC | 1 cut(s) | 2196 |
| Sau96I | GGNCC | 8 cut(s) | 518, 613, 624, 1272, 2031, 2423, 3041, 3352 |
| SbfI | CCTGCAGG | 1 cut(s) | 1005 |
| ScaI | AGTACT | 1 cut(s) | 1300 |
| SchI | GAGTC | 2 cut(s) | 1496, 2290 |
| ScrFI | CCNGG | 5 cut(s) | 622, 871, 1270, 1881, 3287 |
| SdaI | CCTGCAGG | 1 cut(s) | 1005 |
| SduI | GDGCHC | 4 cut(s) | 331, 1215, 3204, 3432 |
| SfaNI | GCATC | 9 cut(s) | 189, 202, 364, 578, 918, 1239, 2356, 2805, 3123 |
| SfcI | CTRYAG | 1 cut(s) | 1001 |
| SfuI | TTCGAA | 1 cut(s) | 702 |
| SinI | GGWCC | 4 cut(s) | 613, 1272, 2423, 3352 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 539, 1725, 2766 |
| SmlI | CTYRAG | 2 cut(s) | 1064, 3205 |
| SmoI | CTYRAG | 2 cut(s) | 1064, 3205 |
| SpeI | ACTAGT | 1 cut(s) | 3005 |
| SphI | GCATGC | 1 cut(s) | 1399 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 1005 |
| SspI | AATATT | 2 cut(s) | 1797, 2553 |
| StyD4I | CCNGG | 5 cut(s) | 620, 869, 1268, 1879, 3285 |
| StyI | CCWWGG | 2 cut(s) | 2441, 2997 |
| TaaI | ACNGT | 7 cut(s) | 576, 948, 1528, 1658, 2522, 2696, 3442 |
| TaiI | ACGT | 5 cut(s) | 434, 666, 1536, 2991, 3369 |
| TaqI | TCGA | 8 cut(s) | 159, 702, 795, 866, 1056, 1082, 1171, 2243 |
| TatI | WGTACW | 6 cut(s) | 852, 1034, 1298, 1319, 1668, 2183 |
| TauI | GCSGC | 4 cut(s) | 547, 594, 1186, 2911 |
| TscAI | CASTG | 8 cut(s) | 748, 951, 1453, 1822, 1903, 2612, 2934, 3437 |
| TseFI | GTSAC | 4 cut(s) | 1628, 1776, 2676, 2805 |
| TseI | GCWGC | 9 cut(s) | 252, 278, 377, 1678, 1739, 2108, 2111, 2645, 3302 |
| Tsp45I | GTSAC | 4 cut(s) | 1628, 1776, 2676, 2805 |
| TspDTI | ATGAA | 6 cut(s) | 1084, 1742, 2234, 2264, 2373, 2970 |
| TspGWI | ACGGA | 5 cut(s) | 107, 559, 1382, 2976, 3123 |
| TspRI | CASTG | 8 cut(s) | 748, 951, 1453, 1822, 1903, 2612, 2934, 3437 |
| Van91I | CCANNNNNTGG | 5 cut(s) | 950, 2385, 2432, 2789, 2933 |
| Vha464I | CTTAAG | 1 cut(s) | 1064 |
| VneI | GTGCAC | 1 cut(s) | 3428 |
| VpaK11BI | GGWCC | 4 cut(s) | 613, 1272, 2423, 3352 |
| XapI | RAATTY | 6 cut(s) | 1007, 1285, 1458, 2446, 3338, 3399 |
| XbaI | TCTAGA | 3 cut(s) | 2194, 2536, 3101 |
| XceI | RCATGY | 4 cut(s) | 544, 1399, 2465, 2633 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1985 |
| XmnI | GAANNNNTTC | 3 cut(s) | 917, 1905, 2344 |
| ZrmI | AGTACT | 1 cut(s) | 1300 |