RchiOBHm_Chr1g0337111

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001590
Physical Location & Seq
Reverse (-)
28771875 .. 28782284
10410 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56503

Sequence Viewer

Length: 3345 bp
ATGAGAAGCCATGAAGGTTCTGTACTCCACCACTTCTCCAAAGCAGCGTCTCAAAACCTCAACAAAATCGCAGTCATACACGCCTCTGGTGACCGCCGCCGTAGCCGCACCTCCGTCACCGTCCAAACCCTCTACGACGGCGACCGCTTCTTCACGTACGCAGAGTTAATCTCCGCCGTCGATTCCCTCGCTTCGCAGCTCCGTCGCAATAACCTTAATTCAGCTTCATCAGAGCTCAGACCGAGAATTCTCGGCATATATATGCCGCCGTCTGTGGAATACATAGTCTCCGTGCTCTCGATTTTGAGGTGCGGCGAGGCGTTTCTGCCTCTGGACCCGTCCTGGCCAAAACAGAGGCTGCTCTCCGTCGTTGCTTCGGCCAATGTCGACCTAATTATCACCTGCACGACGCCGTTTGGTTACGAATTGGACTCCAATTGGCTGGAAAAGAATATCAATCGTAGCTTTCTGCGGTTTTCCATGGGTGGTGGAGAGTGTGTGACAGAGAGTGATGGAGAGCAGCGTAAGAGTGAGAAAGAGAGGCTGTTCTGTTATGTAATGTACACTTCGGGCTCGATGGGGAAGCCGAAAGGTGTCTGTGGAACTGAACAAGGTCTTTGGAATCGGTTTCGGTGGATGCAAGAATTGTATCCATTGAATGGAGAGGAGGTTTTGTTGTTCAAGACAGCAATAAGCTTTGTTGATCATTTGCAAGAATTTCTTAGTGCTATTCTTACTGGTTCTACTTTGGTCATACCTCCTTTCAATCAACTTAAACAGAATGTGTTCTCCGTTCTCGATTTTCTACAGTTTTCTTCTTTGCAAGGTCGAGACTACCAGCAGCTTCTAAGTTCACTGAATTTGTTAGTGCTAAGCGGTGAAGTTTTGCCTTTATCCTTGTGGGATATGCTGTCAAAGAGATTACCAAGGACCTCTATTTTGAATCTGTATGGGAGCACGGAGGTGTCAGGTGATTGTACATATTTTGACTGCAAGAGGTTGCCAATGATTTTGGAGACAGAGACGCTAACAAGTGTTCCAATTGGTATACCTATAGCTGGTTGTGATGTTGTGCTTGTTAATGATGATGATGTACTCAATGAAGGAGAAATTTTTGTTGCTGGTCTCTGCAATGCTTCTGGATACTATTCTGATTCTACTTTTGCTCCTTTGAACACTGTAAAATTACCTCCAGATTCTGTTTGTTCCAGTTCTGTAAATGGACACGAAAGTCAATCATATTTTCAAACTGGTGATTTCGCCAAACAACTTCATAGCGGTGACTTGGTTTTCTTGGGAAGGAAGGACCGCACTATTAAGCTCAATGGGCAGCGTATTGCTTTAGACGAAATTGAACATACATTGGTGGAACATCCAGATGTAACTGATGCTGCTGTTGTTTTTCGTAATGGTCAAGGGGAACTTATGCAACTTGTAGCATTTATAATGTTAAAGGAGGGAAGACCTGATGAAATATTCAGATCTTCCATCAAAAGTTGGATGGTTGACAAACTTCCACTGGCAATGATTCCTGGCCACTTTGTTATAATGGAATCATTTCCTGTGTCTACTAGTGGAAAAGTTGACTATGCTTTGTTAGCAGATTCAGTATTTCTGGCAAAACATATCCAAGATGAGCTTGGTCATATTGGGAGAAGCAATCTACTGCAAGTAATTAAAAAGGCCTTTAGTCGTGTTTTAATAATGGCTGAAGAGGTTTCCGATGATGATGATTTCTTTATGATTGGTGGAGATTCTATTGCTGCTGCACATCTTGCTAACAATATAGGAGTTGATATGAGATTGATATATAGCTTTCCAACTCCATCTAAGCTTTCTATGGCTCTTCTAGAGAGAAAAGAACCATTCAGTATGAAAGTTGAAGTGGATTCTGAATCCGAGATGAATCAGGAAAGGGGAAAGAACATGTTTCATGTCCTCTATGACACTCCTAGTGCTGTTAATCTTGAACAGTTGAAAACTCTGCCTAGAAGGAATGAAAATAAAGCAGTTATGTCTAAACGCTTGAAAGCCGATTCAAGTATAAATGTTGCTTCAGGGAGTACTAGTCCAGCAGATGGGTATCCATGGAGTTCTATGCAAAAATGCATGACATGTTCTTTCAGCAGGTGCAACAAAGTTGTGTATGAGGGCAGTTCCAGGGTGAATGATGTTTGTCAAGCGGCTAGTTCTGTGGTTGTCCCAAAAAGTCTAAAAGTGCATTTGGAAGAATTGTGGAAAGTTGACATGGGATCCTGCGTTGACGCATCACCACTTATTGTTTGTAAAGGCCAGGATATCTACTTATTTATTGGATCACACTCACAAAAGTTTGCGTGTGTTAATGCCAGAAGTGGTTCCATCCAGTGGGAGTTACAACTTGAAGGACGTGTAGAATGTTCGGCAGCAATTCTTGGTGACTTTACTCAGGTTGTAGTTGGATGCTACAAAGGGAAGATATATTTCCTGGATTCTTTGAATGGTAACATATGCTGGACTTTCCAAACATCTGGTGAGGTAAAGTCACAGCCGATTATAGACAGTCAAAGACAATTGATCTGGTGTGGGTCATATGACCGTAACTTGTATGCGCTAGATTATAAAAATCATTGCTGTGTTTATCAGCTTCCATGTGGTGGGAGTATCTGTGGGTCTCCTGCAATAGATGAGATGAATAATATACTTTATGTGGCTTCTACTGGAGGCCAAATGACAGCCATTTCAGTAACGGCATTCCCATTTTCTATTATGTGGGTGCATGAATTAGAAGTGCCAGTATTTGGGTCTCTTGCCATCAATTCTCTGAATGGAAATATTATATGTTGCTTGGTGGATGGGCATGTTCTTGCATTTAATACAAGTGGATCTATTATTTGGAGGTATAGAACTGCTGGGCCTGTATTTGCTGGAGCCTGCATATCTTCTGCTCTTCCCTTTCAGCTACTTGTCTGTTCCAGAGATGGAAACATCTATTCACTCGAACTCGAATCGGGAGGTTTACTCTGGGAATACAATGTTAAGGATCCAATTACTTCATCTGCCTATGTCGATGAGCACCTGAGCTTGGTGTCTGATCAGTCTAACTTTCCCGACCGCAGGGTGGTTTGTGTATGTTCCAGCTCCGGAAGCATGCTTCTGATTCAGGTTAATTTGGATGCCACCGAAGGTGCAAGTCCAGTGAATTTCATTGTTGAGGAATTTGCAAGGTTGGAACTCCCAGGAGATGTATTCTCTTCCCCTGTAATGATTGGTGGCAGAATTTTCGTTGGTTGTAGGGATGATTATGTGCACTGCATTGCTGTTAAGCCCCAGGTCTCAACAGGAACAATGAGTTGTTCTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C PP-binding PQQ PQQ_2 PQQ_3

Protein Analysis

1114

Amino Acids

122.95

Weight (kDa)

5.34

Isoelectric Point (pI)

44.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 41 - 383 3.7e-43 AMP-binding enzyme
AMP-binding_C PF13193 450 - 527 1.7e-09 AMP-binding enzyme C-terminal domain
PQQ_2 PF13360 743 - 866 8.7e-08 Outer membrane protein assembly factor BamB
Beta-prop_ACSF4 PF13570 750 - 1100 4.2e-102 Beta-alanine-activating enzyme, beta-propeller
PQQ_2 PF13360 892 - 1099 2.6e-10 Outer membrane protein assembly factor BamB
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1445, 1547, 2601
AarI CACCTGC 2 cut(s) 410, 2118
AasI GACNNNNNNGTC 1 cut(s) 1230
Acc36I ACCTGC 2 cut(s) 410, 2118
AccB7I CCANNNNNTGG 5 cut(s) 659, 2078, 2367, 2636, 2780
AccI GTMKAC 3 cut(s) 387, 1048, 1568
AccIII TCCGGA 1 cut(s) 3122
AclWI GGATC 6 cut(s) 2247, 2260, 2323, 2872, 3017, 3030
AcoI YGGCCR 3 cut(s) 344, 378, 1534
AcsI RAATTY 7 cut(s) 246, 716, 859, 1110, 3181, 3197, 3258
AcuI CTGAAG 2 cut(s) 1731, 2040
AcyI GRCGYC 1 cut(s) 410
AfaI GTAC 6 cut(s) 24, 158, 563, 979, 1095, 2065
AfiI CCNNNNNNNGG 9 cut(s) 659, 1058, 2078, 2367, 2636, 2780, 3064, 3096, 3100
AflIII ACRYGT 3 cut(s) 1926, 2114, 2389
AhlI ACTAGT 2 cut(s) 1571, 2066
AjiI CACGTC 1 cut(s) 2390
AjnI CCWGG 7 cut(s) 341, 1531, 2159, 2292, 2466, 3217, 3309
AleI CACNNNNGTG 1 cut(s) 962
Alw21I GWGCWC 5 cut(s) 237, 297, 959, 3057, 3291
Alw26I GTCTC 9 cut(s) 54, 292, 825, 1010, 1016, 1130, 2658, 2790, 3319
Alw44I GTGCAC 1 cut(s) 3287
AlwI GGATC 6 cut(s) 2247, 2260, 2323, 2872, 3017, 3030
AlwNI CAGNNNCTG 2 cut(s) 358, 1199
Aor13HI TCCGGA 1 cut(s) 3122
AoxI GGCC 7 cut(s) 344, 378, 1534, 1683, 2290, 2704, 2894
ApaLI GTGCAC 1 cut(s) 3287
ApoI RAATTY 7 cut(s) 246, 716, 859, 1110, 3181, 3197, 3258
ArsI GACNNNNNNTTYG 4 cut(s) 57, 89, 922, 954
Asp700I GAANNNNTTC 3 cut(s) 785, 1557, 2356
AspLEI GCGC 1 cut(s) 2593
AspS9I GGNCC 4 cut(s) 334, 930, 1306, 2894
AvaII GGWCC 3 cut(s) 334, 930, 1306
BaeGI GKGCMC 1 cut(s) 3291
BalI TGGCCA 2 cut(s) 346, 1536
BamHI GGATCC 2 cut(s) 2252, 3022
BanII GRGCYC 2 cut(s) 237, 575
BarI GAAGNNNNNNTAC 1 cut(s) 38
BbsI GAAGAC 1 cut(s) 1468
Bbv12I GWGCWC 5 cut(s) 237, 297, 959, 3057, 3291
BceAI ACGGC 6 cut(s) 84, 154, 161, 253, 397, 2745
BcgI CGANNNNNNTGC 4 cut(s) 185, 219, 3244, 3278
BciT130I CCWGG 7 cut(s) 343, 1533, 2161, 2294, 2468, 3219, 3311
BciVI GTATCC 3 cut(s) 660, 1136, 2094
BclI TGATCA 2 cut(s) 703, 3073
BcoDI GTCTC 9 cut(s) 54, 292, 825, 1010, 1016, 1130, 2658, 2790, 3319
BcuI ACTAGT 2 cut(s) 1571, 2066
BfaI CTAG 7 cut(s) 1572, 1850, 1953, 1989, 2067, 2187, 2594
BfmI CTRYAG 2 cut(s) 806, 1053
BfuAI ACCTGC 2 cut(s) 410, 2118
BfuI GTATCC 3 cut(s) 660, 1136, 2094
BglII AGATCT 1 cut(s) 1481
BlpI GCTNAGC 1 cut(s) 872
BmcAI AGTACT 1 cut(s) 2065
Bme1390I CCNGG 7 cut(s) 343, 1533, 2161, 2294, 2468, 3219, 3311
Bme18I GGWCC 3 cut(s) 334, 930, 1306
BmgBI CACGTC 1 cut(s) 2390
BmgT120I GGNCC 4 cut(s) 334, 930, 1306, 2894
BmiI GGNNCC 5 cut(s) 336, 2254, 2359, 2911, 3024
BmrFI CCNGG 7 cut(s) 343, 1533, 2161, 2294, 2468, 3219, 3311
BmsI GCATC 5 cut(s) 627, 1378, 2276, 2432, 3145
BpiI GAAGAC 1 cut(s) 1468
BplI GAGNNNNNCTC 4 cut(s) 155, 187, 2985, 3017
BpmI CTGGAG 3 cut(s) 1176, 2721, 2928
Bpu10I CCTNAGC 1 cut(s) 3059
Bpu1102I GCTNAGC 1 cut(s) 872
BsaAI YACGTR 1 cut(s) 156
BsaBI GATNNNNATC 1 cut(s) 2082
BsaHI GRCGYC 1 cut(s) 410
BsaI GGTCTC 4 cut(s) 1130, 2658, 2790, 3319
BsaJI CCNNGG 6 cut(s) 480, 926, 2087, 2160, 3217, 3309
BsaWI WCCGGW 1 cut(s) 3122
Bsc4I CCNNNNNNNGG 9 cut(s) 659, 1058, 2078, 2367, 2636, 2780, 3064, 3096, 3100
Bse1I ACTGG 8 cut(s) 742, 1209, 1255, 1524, 2365, 2704, 2774, 3176
Bse3DI GCAATG 4 cut(s) 1138, 1530, 2608, 3294
Bse8I GATNNNNATC 1 cut(s) 2082
BseAI TCCGGA 1 cut(s) 3122
BseBI CCWGG 7 cut(s) 343, 1533, 2161, 2294, 2468, 3219, 3311
BseDI CCNNGG 6 cut(s) 480, 926, 2087, 2160, 3217, 3309
BseGI GGATG 8 cut(s) 642, 1372, 1506, 2361, 2447, 2839, 3160, 3283
BseJI GATNNNNATC 1 cut(s) 2082
BseLI CCNNNNNNNGG 9 cut(s) 659, 1058, 2078, 2367, 2636, 2780, 3064, 3096, 3100
BseMI GCAATG 4 cut(s) 1138, 1530, 2608, 3294
BseMII CTCAG 3 cut(s) 250, 2441, 3050
BseNI ACTGG 8 cut(s) 742, 1209, 1255, 1524, 2365, 2704, 2774, 3176
BseRI GAGGAG 1 cut(s) 680
BseSI GKGCMC 1 cut(s) 3291
BseYI CCCAGC 1 cut(s) 2891
BsgI GTGCAG 2 cut(s) 388, 1752
Bsh1285I CGRYCG 2 cut(s) 145, 3094
BshFI GGCC 7 cut(s) 346, 380, 1536, 1685, 2292, 2706, 2896
BsiEI CGRYCG 2 cut(s) 145, 3094
BsiHKAI GWGCWC 5 cut(s) 237, 297, 959, 3057, 3291
BsiSI CCGG 1 cut(s) 3123
BsiWI CGTACG 1 cut(s) 156
BslFI GGGAC 1 cut(s) 2186
BslI CCNNNNNNNGG 9 cut(s) 659, 1058, 2078, 2367, 2636, 2780, 3064, 3096, 3100
BsmAI GTCTC 9 cut(s) 54, 292, 825, 1010, 1016, 1130, 2658, 2790, 3319
BsmBI CGTCTC 2 cut(s) 54, 1016
BsmFI GGGAC 1 cut(s) 2186
BsmI GAATGC 1 cut(s) 2732
BsnI GGCC 7 cut(s) 346, 380, 1536, 1685, 2292, 2706, 2896
Bso31I GGTCTC 4 cut(s) 1130, 2658, 2790, 3319
Bsp1286I GDGCHC 6 cut(s) 237, 297, 575, 959, 3057, 3291
Bsp13I TCCGGA 1 cut(s) 3122
Bsp1407I TGTACA 2 cut(s) 561, 977
Bsp143I GATC 8 cut(s) 703, 1481, 2252, 2315, 2556, 2864, 3022, 3073
Bsp1720I GCTNAGC 1 cut(s) 872
Bsp19I CCATGG 2 cut(s) 480, 2087
BspANI GGCC 7 cut(s) 346, 380, 1536, 1685, 2292, 2706, 2896
BspCNI CTCAG 3 cut(s) 249, 2440, 3051
BspEI TCCGGA 1 cut(s) 3122
BspLI GGNNCC 5 cut(s) 336, 2254, 2359, 2911, 3024
BspMI ACCTGC 2 cut(s) 410, 2118
BspPI GGATC 6 cut(s) 2247, 2260, 2323, 2872, 3017, 3030
BspQI GCTCTTC 2 cut(s) 1851, 2934
BspTNI GGTCTC 4 cut(s) 1130, 2658, 2790, 3319
BsrDI GCAATG 4 cut(s) 1138, 1530, 2608, 3294
BsrGI TGTACA 2 cut(s) 561, 977
BsrI ACTGG 8 cut(s) 742, 1209, 1255, 1524, 2365, 2704, 2774, 3176
BssECI CCNNGG 6 cut(s) 480, 926, 2087, 2160, 3217, 3309
BssMI GATC 8 cut(s) 703, 1481, 2252, 2315, 2556, 2864, 3022, 3073
BssNAI GTATAC 1 cut(s) 1049
BssNI GRCGYC 1 cut(s) 410
BssT1I CCWWGG 3 cut(s) 480, 926, 2087
Bst1107I GTATAC 1 cut(s) 1049
Bst2UI CCWGG 7 cut(s) 343, 1533, 2161, 2294, 2468, 3219, 3311
Bst4CI ACNGT 6 cut(s) 121, 810, 1180, 1974, 2543, 2579
Bst6I CTCTTC 4 cut(s) 1707, 1851, 2934, 3238
BstACI GRCGYC 1 cut(s) 410
BstAPI GCANNNNNTGC 1 cut(s) 1775
BstAUI TGTACA 2 cut(s) 561, 977
BstBAI YACGTR 1 cut(s) 156
BstC8I GCNNGC 2 cut(s) 2914, 3131
BstDEI CTNAG 7 cut(s) 236, 722, 848, 872, 1830, 2427, 3059
BstDSI CCRYGG 2 cut(s) 480, 2087
BstEII GGTNACC 1 cut(s) 89
BstF5I GGATG 8 cut(s) 642, 1372, 1506, 2361, 2447, 2839, 3160, 3283
BstHHI GCGC 1 cut(s) 2593
BstKTI GATC 8 cut(s) 706, 1484, 2255, 2318, 2559, 2867, 3025, 3076
BstMAI GTCTC 9 cut(s) 54, 292, 825, 1010, 1016, 1130, 2658, 2790, 3319
BstMBI GATC 8 cut(s) 703, 1481, 2252, 2315, 2556, 2864, 3022, 3073
BstMCI CGRYCG 2 cut(s) 145, 3094
BstMWI GCNNNNNNNGC 6 cut(s) 102, 105, 1327, 1598, 1775, 3126
BstNI CCWGG 7 cut(s) 343, 1533, 2161, 2294, 2468, 3219, 3311
BstNSI RCATGY 4 cut(s) 1930, 2118, 2843, 3133
BstPI GGTNACC 1 cut(s) 89
BstSCI CCNGG 7 cut(s) 341, 1531, 2159, 2292, 2466, 3217, 3309
BstSFI CTRYAG 2 cut(s) 806, 1053
BstSLI GKGCMC 1 cut(s) 3291
BstV2I GAAGAC 1 cut(s) 1468
BstX2I RGATCY 4 cut(s) 1481, 2252, 2864, 3022
BstXI CCANNNNNNTGG 2 cut(s) 442, 2510
BstYI RGATCY 4 cut(s) 1481, 2252, 2864, 3022
BstZ17I GTATAC 1 cut(s) 1049
BsuI GTATCC 3 cut(s) 660, 1136, 2094
BsuRI GGCC 7 cut(s) 346, 380, 1536, 1685, 2292, 2706, 2896
BtgI CCRYGG 2 cut(s) 480, 2087
BtrI CACGTC 1 cut(s) 2390
BtsCI GGATG 8 cut(s) 642, 1372, 1506, 2361, 2447, 2839, 3160, 3283
BtsI GCAGTG 1 cut(s) 3289
BtsIMutI CAGTG 6 cut(s) 854, 1176, 1517, 2372, 3183, 3289
BveI ACCTGC 2 cut(s) 410, 2118
Cac8I GCNNGC 2 cut(s) 2914, 3131
CaiI CAGNNNCTG 2 cut(s) 358, 1199
CfoI GCGC 1 cut(s) 2593
Cfr13I GGNCC 4 cut(s) 334, 930, 1306, 2894
CseI GACGC 4 cut(s) 36, 418, 1033, 2273
Csp6I GTAC 6 cut(s) 23, 157, 562, 978, 1094, 2064
CviQI GTAC 6 cut(s) 23, 157, 562, 978, 1094, 2064
DdeI CTNAG 7 cut(s) 236, 722, 848, 872, 1830, 2427, 3059
DpnI GATC 8 cut(s) 705, 1483, 2254, 2317, 2558, 2866, 3024, 3075
DpnII GATC 8 cut(s) 703, 1481, 2252, 2315, 2556, 2864, 3022, 3073
DrdI GACNNNNNNGTC 1 cut(s) 1230
DseDI GACNNNNNNGTC 1 cut(s) 1230
EaeI YGGCCR 3 cut(s) 344, 378, 1534
Eam1104I CTCTTC 4 cut(s) 1707, 1851, 2934, 3238
EarI CTCTTC 4 cut(s) 1707, 1851, 2934, 3238
EciI GGCGGA 1 cut(s) 163
Ecl136II GAGCTC 1 cut(s) 235
Eco130I CCWWGG 3 cut(s) 480, 926, 2087
Eco147I AGGCCT 1 cut(s) 1685
Eco24I GRGCYC 2 cut(s) 237, 575
Eco31I GGTCTC 4 cut(s) 1130, 2658, 2790, 3319
Eco32I GATATC 1 cut(s) 2299
Eco47I GGWCC 3 cut(s) 334, 930, 1306
Eco53kI GAGCTC 1 cut(s) 235
Eco57I CTGAAG 2 cut(s) 1731, 2040
Eco91I GGTNACC 1 cut(s) 89
EcoICRI GAGCTC 1 cut(s) 235
EcoO109I RGGNCCY 1 cut(s) 930
EcoO65I GGTNACC 1 cut(s) 89
EcoRI GAATTC 1 cut(s) 246
EcoRII CCWGG 7 cut(s) 341, 1531, 2159, 2292, 2466, 3217, 3309
EcoRV GATATC 1 cut(s) 2299
EcoT14I CCWWGG 3 cut(s) 480, 926, 2087
EcoT22I ATGCAT 1 cut(s) 2111
EcoT38I GRGCYC 2 cut(s) 237, 575
ErhI CCWWGG 3 cut(s) 480, 926, 2087
Esp3I CGTCTC 2 cut(s) 54, 1016
FalI AAGNNNNNCTT 6 cut(s) 705, 737, 1407, 1439, 1623, 1655
FaqI GGGAC 1 cut(s) 2186
FauNDI CATATG 2 cut(s) 2489, 2572
FbaI TGATCA 2 cut(s) 703, 3073
FblI GTMKAC 3 cut(s) 387, 1048, 1568
FokI GGATG 8 cut(s) 649, 1359, 1513, 2348, 2454, 2846, 3167, 3290
FriOI GRGCYC 2 cut(s) 237, 575
FspBI CTAG 7 cut(s) 1572, 1850, 1953, 1989, 2067, 2187, 2594
GlaI GCGC 1 cut(s) 2592
GsaI CCCAGC 1 cut(s) 2895
GsuI CTGGAG 3 cut(s) 1176, 2721, 2928
HaeIII GGCC 7 cut(s) 346, 380, 1536, 1685, 2292, 2706, 2896
HapII CCGG 1 cut(s) 3123
HgaI GACGC 4 cut(s) 36, 418, 1033, 2273
HhaI GCGC 1 cut(s) 2593
Hin1I GRCGYC 1 cut(s) 410
Hin6I GCGC 1 cut(s) 2591
HinP1I GCGC 1 cut(s) 2591
HincII GTYRAC 5 cut(s) 388, 1507, 1585, 2245, 2263
HindII GTYRAC 5 cut(s) 388, 1507, 1585, 2245, 2263
HindIII AAGCTT 2 cut(s) 694, 1832
HpaII CCGG 1 cut(s) 3123
Hpy99I CGWCG 5 cut(s) 140, 182, 207, 371, 412
HpyAV CCTTC 7 cut(s) 8, 1097, 1293, 1297, 1986, 2378, 3158
HpyCH4III ACNGT 6 cut(s) 121, 810, 1180, 1974, 2543, 2579
HpyCH4IV ACGT 2 cut(s) 155, 2389
HpyF10VI GCNNNNNNNGC 6 cut(s) 102, 105, 1327, 1598, 1775, 3126
HpyF3I CTNAG 7 cut(s) 236, 722, 848, 872, 1830, 2427, 3059
HpySE526I ACGT 2 cut(s) 155, 2389
Hsp92I GRCGYC 1 cut(s) 410
HspAI GCGC 1 cut(s) 2591
Kpn2I TCCGGA 1 cut(s) 3122
Ksp22I TGATCA 2 cut(s) 703, 3073
Kzo9I GATC 8 cut(s) 703, 1481, 2252, 2315, 2556, 2864, 3022, 3073
LguI GCTCTTC 2 cut(s) 1851, 2934
LmnI GCTCC 5 cut(s) 204, 954, 1171, 2909, 3125
LweI GCATC 5 cut(s) 627, 1378, 2276, 2432, 3145
MaeI CTAG 7 cut(s) 1572, 1850, 1953, 1989, 2067, 2187, 2594
MaeII ACGT 2 cut(s) 155, 2389
MalI GATC 8 cut(s) 705, 1483, 2254, 2317, 2558, 2866, 3024, 3075
MboI GATC 8 cut(s) 703, 1481, 2252, 2315, 2556, 2864, 3022, 3073
MfeI CAATTG 3 cut(s) 436, 1041, 2552
MflI RGATCY 4 cut(s) 1481, 2252, 2864, 3022
MhlI GDGCHC 6 cut(s) 237, 297, 575, 959, 3057, 3291
MlsI TGGCCA 2 cut(s) 346, 1536
MluNI TGGCCA 2 cut(s) 346, 1536
MlyI GAGTC 1 cut(s) 425
MmeI TCCRAC 4 cut(s) 1478, 1844, 2419, 3189
Mox20I TGGCCA 2 cut(s) 346, 1536
Mph1103I ATGCAT 1 cut(s) 2111
MroI TCCGGA 1 cut(s) 3122
MroXI GAANNNNTTC 3 cut(s) 785, 1557, 2356
MscI TGGCCA 2 cut(s) 346, 1536
MslI CAYNNNNRTG 5 cut(s) 260, 962, 1278, 1377, 2613
Msp20I TGGCCA 2 cut(s) 346, 1536
MspI CCGG 1 cut(s) 3123
MspR9I CCNGG 7 cut(s) 343, 1533, 2161, 2294, 2468, 3219, 3311
MunI CAATTG 3 cut(s) 436, 1041, 2552
Mva1269I GAATGC 1 cut(s) 2732
MvaI CCWGG 7 cut(s) 343, 1533, 2161, 2294, 2468, 3219, 3311
MwoI GCNNNNNNNGC 6 cut(s) 102, 105, 1327, 1598, 1775, 3126
NcoI CCATGG 2 cut(s) 480, 2087
NdeI CATATG 2 cut(s) 2489, 2572
NdeII GATC 8 cut(s) 703, 1481, 2252, 2315, 2556, 2864, 3022, 3073
NlaIV GGNNCC 5 cut(s) 336, 2254, 2359, 2911, 3024
NmeAIII GCCGAG 1 cut(s) 231
NmuCI GTSAC 6 cut(s) 89, 115, 499, 1280, 2417, 2523
NsiI ATGCAT 1 cut(s) 2111
NspI RCATGY 4 cut(s) 1930, 2118, 2843, 3133
OliI CACNNNNGTG 1 cut(s) 962
PaeI GCATGC 1 cut(s) 3133
PaqCI CACCTGC 2 cut(s) 410, 2118
PceI AGGCCT 1 cut(s) 1685
PciI ACATGT 2 cut(s) 1926, 2114
PciSI GCTCTTC 2 cut(s) 1851, 2934
PctI GAATGC 1 cut(s) 2732
PdmI GAANNNNTTC 3 cut(s) 785, 1557, 2356
Pfl23II CGTACG 1 cut(s) 156
PflMI CCANNNNNTGG 5 cut(s) 659, 2078, 2367, 2636, 2780
PfoI TCCNGGA 1 cut(s) 2466
PleI GAGTC 1 cut(s) 425
PpsI GAGTC 1 cut(s) 425
Ppu21I YACGTR 1 cut(s) 156
PpuMI RGGWCCY 1 cut(s) 930
PscI ACATGT 2 cut(s) 1926, 2114
PsiI TTATAA 3 cut(s) 1445, 1547, 2601
Psp124BI GAGCTC 1 cut(s) 237
Psp5II RGGWCCY 1 cut(s) 930
Psp6I CCWGG 7 cut(s) 341, 1531, 2159, 2292, 2466, 3217, 3309
PspEI GGTNACC 1 cut(s) 89
PspFI CCCAGC 1 cut(s) 2891
PspGI CCWGG 7 cut(s) 341, 1531, 2159, 2292, 2466, 3217, 3309
PspLI CGTACG 1 cut(s) 156
PspN4I GGNNCC 5 cut(s) 336, 2254, 2359, 2911, 3024
PspPI GGNCC 4 cut(s) 334, 930, 1306, 2894
PspPPI RGGWCCY 1 cut(s) 930
PstNI CAGNNNCTG 2 cut(s) 358, 1199
PsuI RGATCY 4 cut(s) 1481, 2252, 2864, 3022
RsaI GTAC 6 cut(s) 24, 158, 563, 979, 1095, 2065
RsaNI GTAC 6 cut(s) 23, 157, 562, 978, 1094, 2064
RseI CAYNNNNRTG 5 cut(s) 260, 962, 1278, 1377, 2613
SacI GAGCTC 1 cut(s) 237
SalI GTCGAC 1 cut(s) 386
SapI GCTCTTC 2 cut(s) 1851, 2934
Sau3AI GATC 8 cut(s) 703, 1481, 2252, 2315, 2556, 2864, 3022, 3073
Sau96I GGNCC 4 cut(s) 334, 930, 1306, 2894
ScaI AGTACT 1 cut(s) 2065
SchI GAGTC 1 cut(s) 425
ScrFI CCNGG 7 cut(s) 343, 1533, 2161, 2294, 2468, 3219, 3311
SduI GDGCHC 6 cut(s) 237, 297, 575, 959, 3057, 3291
SfaNI GCATC 5 cut(s) 627, 1378, 2276, 2432, 3145
SfcI CTRYAG 2 cut(s) 806, 1053
SinI GGWCC 3 cut(s) 334, 930, 1306
SmiMI CAYNNNNRTG 5 cut(s) 260, 962, 1278, 1377, 2613
SpeI ACTAGT 2 cut(s) 1571, 2066
SphI GCATGC 1 cut(s) 3133
SseBI AGGCCT 1 cut(s) 1685
SspI AATATT 2 cut(s) 1476, 2815
SspMI CTAG 7 cut(s) 1572, 1850, 1953, 1989, 2067, 2187, 2594
SstI GAGCTC 1 cut(s) 237
StuI AGGCCT 1 cut(s) 1685
StyD4I CCNGG 7 cut(s) 341, 1531, 2159, 2292, 2466, 3217, 3309
StyI CCWWGG 3 cut(s) 480, 926, 2087
TaaI ACNGT 6 cut(s) 121, 810, 1180, 1974, 2543, 2579
TaiI ACGT 2 cut(s) 158, 2392
TaqI TCGA 9 cut(s) 180, 299, 387, 575, 798, 829, 2979, 2985, 3048
TaqII GACCGA 1 cut(s) 256
TatI WGTACW 5 cut(s) 22, 561, 977, 1093, 2063
TauI GCSGC 5 cut(s) 99, 108, 268, 315, 2186
TscAI CASTG 6 cut(s) 861, 1183, 1524, 2372, 3183, 3296
TseFI GTSAC 6 cut(s) 89, 115, 499, 1280, 2417, 2523
Tsp45I GTSAC 6 cut(s) 89, 115, 499, 1280, 2417, 2523
TspGWI ACGGA 6 cut(s) 103, 191, 280, 355, 781, 974
TspRI CASTG 6 cut(s) 861, 1183, 1524, 2372, 3183, 3296
Van91I CCANNNNNTGG 5 cut(s) 659, 2078, 2367, 2636, 2780
VneI GTGCAC 1 cut(s) 3287
VpaK11BI GGWCC 3 cut(s) 334, 930, 1306
XapI RAATTY 7 cut(s) 246, 716, 859, 1110, 3181, 3197, 3258
XbaI TCTAGA 1 cut(s) 1849
XceI RCATGY 4 cut(s) 1930, 2118, 2843, 3133
XcmI CCANNNNNNNNNTGG 1 cut(s) 1637
XmiI GTMKAC 3 cut(s) 387, 1048, 1568
XmnI GAANNNNTTC 3 cut(s) 785, 1557, 2356
XspI CTAG 7 cut(s) 1572, 1850, 1953, 1989, 2067, 2187, 2594
ZrmI AGTACT 1 cut(s) 2065
Zsp2I ATGCAT 1 cut(s) 2111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.