Rroxscaffold_4G00314950

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001590
Physical Location & Seq
Forward (+)
39171037 .. 39180959
9923 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00314950.1

Sequence Viewer

Length: 2832 bp
ATGAGAAGCCACGGAGGTTCTATATTCCACCACTTCTCCAGAGCAGCGTCCCAAAACCCCAACAAAATCGCAGTCATACACGCCTCCGGTGACCGCCGTCGTACCCGCACCTCCGTCACCGTCCAACCCCTCTACGACGGCGACCGCTCCTTCACGTATGCAGAGTTAATCTCCGCCGTCGATTCCCTCGCTTCGCATCTCCGTCGCAATAACCTTGATTCAGCTTCATCAGAGCGTCAACCGAGAATTCTCGGCATATATATGCCGCCGTCGGTGGAATACATCGTCTCCGTGCTCTCGGTTTTGAGGTGCGGCGAGGCGTTTCTGCCTCTGGACCCGTCCTGGCCAAAACAGAGGCTGCTCTCCGTCGTTGCTTCGGCCAATGTCGACCTAATTATCACCTGCACGACGCCGTTTGGTTACGAATTGGACTCCAATTGGCTGGAAAAGAATTCCAATCGTAGCTTTCTGTGGTTTTCCATGGGTGGTGGAGAGTCTGTGACAGTGAGTGATGGAGAGCAGCGTAAGAGTGAGAAAGAGAGGCTGTTCTGTTATGTAATGTACACTTCGGGCTCGACGGGGAAGCCCAAAGGTGTCTGTGGCACTGAACAAGGTCTTTGGAATCGGTTTCGGTGGATGCAAGAATTGTATCCACTGAATGGAGAGGAAGTTTTGTTGTTCAAGACGGCAATAAGCTTTGTTGATCATTTGCAAGAATTTCTTAGTGCTATTCTTACTGGTTCTACTTTGGTTATACCTCCTTTCAATCAGCTTAAACAGAATGTGTTCTCCATTGTCGATTTTCTGCAGGCTTATTCTATCAATAGACTTACTGCTGTTCCATCACTGATGAGAGTAATCCTTCCACCTTTGCAAGGTCGAGACTACCAGCAGCTTCTAAGTTCACTGAATTTGTTAGTGCTAAGCGGCGAAGTTTTGCCTTTATCCTTGTGGGATATGCTGTCAAAGAGATTACCAAGGACCTCTATTTTGAATCTGTATGGGAGCACGGAGGTGTCAGGTGATTGTACATATTTTGACTGCAAGAGGTTGCCAATGATTTTGGAGACAGAGACGCTAACAAGTGTTCCAATTGGTATACCTATAGCTGGTTGTGATGTTGTGCTTGTTAGTGATGATGATGTACTCAATGAAGGAGAAATTTTTGTTGCTGGTCTCTGCAATGCTTCTGGATACTATTCTGATTCTACTTTTGCTCCTTTGAACACTGTAAAATTACCTCCAGATTCTGTTTGTTCCAGTTCTGTAAATGGACACGAAAGTCAATCATATTTTCAAACTGGTGATTTTGCCAAACAACTTCATAGCGGTGACTTGGTTTTCTTGGGAAGGAAGGACCGCACTATTAAGCTCAATGGGCAGCGTATTGCTTTAGAAGAAATTGAACATGCATTGGTGGAACATCCAGATGTAACTGATGCTGCTGTTGTTTTTCATAATGGTCAAGGGGAACTTATGCAACTTGTAGCATTTATAATGTTAAAGGAGGGAAGACCTGATGAAATATTCAGATCTTCCATCAAAAGTTGGATGGTTGACAAACTTCCACTGGCAATGATTCCTGGCCACTTTGTTATAATGGAATCATTTCCTGTGTCTACTAGTGGAAAAGTTGACTATGCTTTGTTAGCAGATTCAGTATTTCTGGCAAAACATATCCAAGATGAGCTTGGTCATATTGGGAGAAGCAATCTACTGCAAGTAATTAAAAAGGCCTTTAGTCGTGTTTTAATAATGGCTGAAGAGGTTTCCGATGATGATGATTTCTTTATGATTGGTGGAAATTCTATTGCTGCTGCACATCTTGCTAACAATATAGGAGTTGATATGAGATTGATATATAGCTTTCCAACTCCATCTAAGCTTTCTATGGCTCTTCTAGAGAGAAAAGAACCATTCAGTATGAAAGTTGAAGTGGATTCTGAATCCGAGATGAATCAGGAAAGGGGAAAGCACATGTTTCATGTCCTCTATGACACTCCTAGTGCTGTTAATCTTGAACAGTTGAAAACTCTGCCTAGAAGGAATGAAAATAAAGCAGTTATGTCTAAACGCTTGAAAGCCGATTCAAGTATAAATGTTGCTTCAGGGAGTACTAGTCCAGCAGATGGGTATCCATGGAGTTCTGTGCAAAAATGCATGACATGTTCTTTCAGCAGGTGCAACAAAGTTGTGTATGAGGGCAGTTCCAGGGTGAATGATGTTTGTCAAGCGGCTAGTTCTGTGGTTGTCCCAAAAAGTCTGAAAGTGCATTTGGAAGAATTGTGGAAAGTTGACATGGGATCCTGCGTTGACGCATCACCACTTATTGTTTGTAAAGGCCAGGATATCTACTTATTTATTGGATCACACTCACAAAAGTTTGTGTGTGTTAATGCCAGAAGGTTACCGCTTCAGGTTGTAGTTGGATGCTACAAAGGGAAGATATATTTCCTGGATTCTTTGAATGGTAACATATGTTGGACTTTCCAAACGTCTGGTGAGCTTCCATGTGGTGGGAGTATCTGTGGGTCTCCTGCAATAGATGAGATGAATAATATACTTTATGTGGCTTCTACTGGAGGCCAAATGACAGCCATTTCAGTAACGTATAGAACTGCTGGGCCTGTATTTGCTGGAGCCTGCATATCTTTTGCTCTTCCCTTTCAGCTACTTGTCTGTTCCAGAGATGGAAACATCTATTCACTCGAACTCGAATCGGGAGGGTTACTCTGGGAATACAAAGTTAAGGATCCAATTACTTCATCTGCCTATGTCGATGAGCACCTGAGCTTGGTGTCTGATCAGTCTAATTTTCCTGACAGCAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C PP-binding PQQ PQQ_2 PQQ_3

Protein Analysis

943

Amino Acids

104.15

Weight (kDa)

5.75

Isoelectric Point (pI)

44.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 12 - 400 2.4e-55 AMP-binding enzyme
AMP-binding_C PF13193 467 - 544 3e-10 AMP-binding enzyme C-terminal domain
Beta-prop_ACSF4 PF13570 767 - 801 9.1e-08 Beta-alanine-activating enzyme, beta-propeller
Beta-prop_ACSF4 PF13570 806 - 933 7.8e-19 Beta-alanine-activating enzyme, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1496, 1598
AarI CACCTGC 2 cut(s) 410, 2169
AasI GACNNNNNNGTC 1 cut(s) 1281
Acc36I ACCTGC 3 cut(s) 410, 2169, 2817
AccB7I CCANNNNNTGG 3 cut(s) 659, 2129, 2516
AccBSI CCGCTC 1 cut(s) 147
AccI GTMKAC 3 cut(s) 387, 1099, 1619
AclWI GGATC 5 cut(s) 2298, 2311, 2374, 2747, 2760
AcoI YGGCCR 3 cut(s) 344, 378, 1585
AcsI RAATTY 6 cut(s) 246, 451, 716, 910, 1161, 1804
AcuI CTGAAG 3 cut(s) 1782, 2091, 2399
AcyI GRCGYC 1 cut(s) 410
AfaI GTAC 5 cut(s) 103, 563, 1030, 1146, 2116
AfiI CCNNNNNNNGG 6 cut(s) 659, 875, 1109, 2129, 2516, 2794
AflIII ACRYGT 2 cut(s) 1977, 2165
AhlI ACTAGT 2 cut(s) 1622, 2117
AjnI CCWGG 5 cut(s) 341, 1582, 2210, 2343, 2454
AjuI GAANNNNNNNTTGG 2 cut(s) 2258, 2290
AleI CACNNNNGTG 1 cut(s) 1013
Alw21I GWGCWC 3 cut(s) 297, 1010, 2787
Alw26I GTCTC 6 cut(s) 292, 876, 1061, 1067, 1181, 2538
AlwI GGATC 5 cut(s) 2298, 2311, 2374, 2747, 2760
AlwNI CAGNNNCTG 2 cut(s) 358, 1250
AoxI GGCC 7 cut(s) 344, 378, 1585, 1734, 2341, 2584, 2624
ApeKI GCWGC 8 cut(s) 44, 358, 520, 892, 1381, 1442, 1814, 1817
ApoI RAATTY 6 cut(s) 246, 451, 716, 910, 1161, 1804
ArsI GACNNNNNNTTYG 4 cut(s) 57, 89, 973, 1005
Asp700I GAANNNNTTC 2 cut(s) 785, 1608
AspS9I GGNCC 4 cut(s) 334, 981, 1357, 2624
AsuHPI GGTGA 9 cut(s) 101, 109, 391, 1034, 1316, 1343, 2227, 2313, 2513
AvaII GGWCC 3 cut(s) 334, 981, 1357
BalI TGGCCA 2 cut(s) 346, 1587
BamHI GGATCC 2 cut(s) 2303, 2752
BanII GRGCYC 1 cut(s) 575
BbsI GAAGAC 1 cut(s) 1519
Bbv12I GWGCWC 3 cut(s) 297, 1010, 2787
BbvI GCAGC 8 cut(s) 56, 345, 532, 904, 1393, 1429, 1801, 1804
BccI CCATC 7 cut(s) 506, 850, 1546, 1547, 1885, 2123, 2684
BceAI ACGGC 6 cut(s) 81, 154, 161, 253, 397, 702
BcgI CGANNNNNNTGC 2 cut(s) 185, 219
BciT130I CCWGG 5 cut(s) 343, 1584, 2212, 2345, 2456
BciVI GTATCC 3 cut(s) 660, 1187, 2145
BclI TGATCA 2 cut(s) 703, 2803
BcoDI GTCTC 6 cut(s) 292, 876, 1061, 1067, 1181, 2538
BcuI ACTAGT 2 cut(s) 1622, 2117
BfaI CTAG 6 cut(s) 1623, 1901, 2004, 2040, 2118, 2238
BfmI CTRYAG 2 cut(s) 806, 1104
BfuAI ACCTGC 3 cut(s) 410, 2169, 2817
BfuI GTATCC 3 cut(s) 660, 1187, 2145
BglII AGATCT 1 cut(s) 1532
BlpI GCTNAGC 1 cut(s) 923
BmcAI AGTACT 1 cut(s) 2116
Bme1390I CCNGG 5 cut(s) 343, 1584, 2212, 2345, 2456
Bme18I GGWCC 3 cut(s) 334, 981, 1357
BmgT120I GGNCC 4 cut(s) 334, 981, 1357, 2624
BmiI GGNNCC 4 cut(s) 336, 2305, 2641, 2754
BmrFI CCNGG 5 cut(s) 343, 1584, 2212, 2345, 2456
BmsI GCATC 5 cut(s) 205, 627, 1429, 2327, 2420
BoxI GACNNNNGTC 1 cut(s) 96
BpiI GAAGAC 1 cut(s) 1519
BplI GAGNNNNNCTC 4 cut(s) 155, 187, 2715, 2747
BpmI CTGGAG 4 cut(s) 22, 1227, 2601, 2658
Bpu10I CCTNAGC 1 cut(s) 2789
Bpu1102I GCTNAGC 1 cut(s) 923
BsaAI YACGTR 1 cut(s) 156
BsaBI GATNNNNATC 1 cut(s) 2133
BsaHI GRCGYC 1 cut(s) 410
BsaI GGTCTC 2 cut(s) 1181, 2538
BsaJI CCNNGG 5 cut(s) 10, 480, 977, 2138, 2211
BsaWI WCCGGW 1 cut(s) 86
Bsc4I CCNNNNNNNGG 6 cut(s) 659, 875, 1109, 2129, 2516, 2794
Bse1I ACTGG 5 cut(s) 742, 1260, 1306, 1575, 2584
Bse3DI GCAATG 2 cut(s) 1189, 1581
Bse8I GATNNNNATC 1 cut(s) 2133
BseBI CCWGG 5 cut(s) 343, 1584, 2212, 2345, 2456
BseDI CCNNGG 5 cut(s) 10, 480, 977, 2138, 2211
BseGI GGATG 4 cut(s) 642, 1423, 1557, 2435
BseJI GATNNNNATC 1 cut(s) 2133
BseLI CCNNNNNNNGG 6 cut(s) 659, 875, 1109, 2129, 2516, 2794
BseMI GCAATG 2 cut(s) 1189, 1581
BseMII CTCAG 1 cut(s) 2780
BseNI ACTGG 5 cut(s) 742, 1260, 1306, 1575, 2584
BseXI GCAGC 8 cut(s) 56, 345, 532, 904, 1393, 1429, 1801, 1804
BseYI CCCAGC 1 cut(s) 2621
BsgI GTGCAG 2 cut(s) 388, 1803
Bsh1285I CGRYCG 1 cut(s) 145
BshFI GGCC 7 cut(s) 346, 380, 1587, 1736, 2343, 2586, 2626
BsiEI CGRYCG 1 cut(s) 145
BsiHKAI GWGCWC 3 cut(s) 297, 1010, 2787
BsiSI CCGG 1 cut(s) 87
BslFI GGGAC 2 cut(s) 34, 2237
BslI CCNNNNNNNGG 6 cut(s) 659, 875, 1109, 2129, 2516, 2794
BsmAI GTCTC 6 cut(s) 292, 876, 1061, 1067, 1181, 2538
BsmBI CGTCTC 2 cut(s) 292, 1067
BsmFI GGGAC 2 cut(s) 34, 2237
BsnI GGCC 7 cut(s) 346, 380, 1587, 1736, 2343, 2586, 2626
Bso31I GGTCTC 2 cut(s) 1181, 2538
Bsp1286I GDGCHC 4 cut(s) 297, 575, 1010, 2787
Bsp1407I TGTACA 2 cut(s) 561, 1028
Bsp143I GATC 6 cut(s) 703, 1532, 2303, 2366, 2752, 2803
Bsp1720I GCTNAGC 1 cut(s) 923
Bsp19I CCATGG 2 cut(s) 480, 2138
BspANI GGCC 7 cut(s) 346, 380, 1587, 1736, 2343, 2586, 2626
BspCNI CTCAG 1 cut(s) 2781
BspLI GGNNCC 4 cut(s) 336, 2305, 2641, 2754
BspMAI CTGCAG 1 cut(s) 810
BspMI ACCTGC 3 cut(s) 410, 2169, 2817
BspPI GGATC 5 cut(s) 2298, 2311, 2374, 2747, 2760
BspQI GCTCTTC 2 cut(s) 1902, 2664
BspTNI GGTCTC 2 cut(s) 1181, 2538
BsrBI CCGCTC 1 cut(s) 147
BsrDI GCAATG 2 cut(s) 1189, 1581
BsrGI TGTACA 2 cut(s) 561, 1028
BsrI ACTGG 5 cut(s) 742, 1260, 1306, 1575, 2584
BssECI CCNNGG 5 cut(s) 10, 480, 977, 2138, 2211
BssMI GATC 6 cut(s) 703, 1532, 2303, 2366, 2752, 2803
BssNAI GTATAC 1 cut(s) 1100
BssNI GRCGYC 1 cut(s) 410
BssT1I CCWWGG 3 cut(s) 480, 977, 2138
Bst1107I GTATAC 1 cut(s) 1100
Bst2UI CCWGG 5 cut(s) 343, 1584, 2212, 2345, 2456
Bst4CI ACNGT 4 cut(s) 121, 505, 1231, 2025
Bst6I CTCTTC 3 cut(s) 1758, 1902, 2664
BstACI GRCGYC 1 cut(s) 410
BstAPI GCANNNNNTGC 1 cut(s) 1826
BstAUI TGTACA 2 cut(s) 561, 1028
BstBAI YACGTR 1 cut(s) 156
BstC8I GCNNGC 2 cut(s) 810, 2644
BstDEI CTNAG 5 cut(s) 722, 899, 923, 1881, 2789
BstDSI CCRYGG 3 cut(s) 10, 480, 2138
BstEII GGTNACC 2 cut(s) 89, 2406
BstENI CCTNNNNNAGG 1 cut(s) 873
BstF5I GGATG 4 cut(s) 642, 1423, 1557, 2435
BstKTI GATC 6 cut(s) 706, 1535, 2306, 2369, 2755, 2806
BstMAI GTCTC 6 cut(s) 292, 876, 1061, 1067, 1181, 2538
BstMBI GATC 6 cut(s) 703, 1532, 2303, 2366, 2752, 2803
BstMCI CGRYCG 1 cut(s) 145
BstMWI GCNNNNNNNGC 3 cut(s) 1378, 1649, 1826
BstNI CCWGG 5 cut(s) 343, 1584, 2212, 2345, 2456
BstNSI RCATGY 3 cut(s) 1412, 1981, 2169
BstPAI GACNNNNGTC 1 cut(s) 96
BstPI GGTNACC 2 cut(s) 89, 2406
BstSCI CCNGG 5 cut(s) 341, 1582, 2210, 2343, 2454
BstSFI CTRYAG 2 cut(s) 806, 1104
BstV1I GCAGC 8 cut(s) 56, 345, 532, 904, 1393, 1429, 1801, 1804
BstV2I GAAGAC 1 cut(s) 1519
BstX2I RGATCY 3 cut(s) 1532, 2303, 2752
BstXI CCANNNNNNTGG 2 cut(s) 442, 2498
BstYI RGATCY 3 cut(s) 1532, 2303, 2752
BstZ17I GTATAC 1 cut(s) 1100
BsuI GTATCC 3 cut(s) 660, 1187, 2145
BsuRI GGCC 7 cut(s) 346, 380, 1587, 1736, 2343, 2586, 2626
BtgI CCRYGG 3 cut(s) 10, 480, 2138
BtsCI GGATG 4 cut(s) 642, 1423, 1557, 2435
BtsIMutI CAGTG 7 cut(s) 510, 603, 653, 845, 905, 1227, 1568
BveI ACCTGC 3 cut(s) 410, 2169, 2817
Cac8I GCNNGC 2 cut(s) 810, 2644
CaiI CAGNNNCTG 2 cut(s) 358, 1250
Cfr13I GGNCC 4 cut(s) 334, 981, 1357, 2624
CseI GACGC 5 cut(s) 36, 224, 418, 1084, 2324
Csp6I GTAC 5 cut(s) 102, 562, 1029, 1145, 2115
CviAII CATG 9 cut(s) 481, 1409, 1978, 1985, 2139, 2161, 2166, 2299, 2511
CviQI GTAC 5 cut(s) 102, 562, 1029, 1145, 2115
DdeI CTNAG 5 cut(s) 722, 899, 923, 1881, 2789
DpnI GATC 6 cut(s) 705, 1534, 2305, 2368, 2754, 2805
DpnII GATC 6 cut(s) 703, 1532, 2303, 2366, 2752, 2803
DrdI GACNNNNNNGTC 1 cut(s) 1281
DseDI GACNNNNNNGTC 1 cut(s) 1281
EaeI YGGCCR 3 cut(s) 344, 378, 1585
Eam1104I CTCTTC 3 cut(s) 1758, 1902, 2664
EarI CTCTTC 3 cut(s) 1758, 1902, 2664
EciI GGCGGA 1 cut(s) 163
Eco130I CCWWGG 3 cut(s) 480, 977, 2138
Eco147I AGGCCT 1 cut(s) 1736
Eco24I GRGCYC 1 cut(s) 575
Eco31I GGTCTC 2 cut(s) 1181, 2538
Eco32I GATATC 1 cut(s) 2350
Eco47I GGWCC 3 cut(s) 334, 981, 1357
Eco57I CTGAAG 3 cut(s) 1782, 2091, 2399
Eco91I GGTNACC 2 cut(s) 89, 2406
EcoNI CCTNNNNNAGG 1 cut(s) 873
EcoO109I RGGNCCY 1 cut(s) 981
EcoO65I GGTNACC 2 cut(s) 89, 2406
EcoRI GAATTC 2 cut(s) 246, 451
EcoRII CCWGG 5 cut(s) 341, 1582, 2210, 2343, 2454
EcoRV GATATC 1 cut(s) 2350
EcoT14I CCWWGG 3 cut(s) 480, 977, 2138
EcoT22I ATGCAT 2 cut(s) 1414, 2162
EcoT38I GRGCYC 1 cut(s) 575
ErhI CCWWGG 3 cut(s) 480, 977, 2138
Esp3I CGTCTC 2 cut(s) 292, 1067
FaeI CATG 9 cut(s) 484, 1412, 1981, 1988, 2142, 2164, 2169, 2302, 2514
FalI AAGNNNNNCTT 6 cut(s) 705, 737, 1458, 1490, 1674, 1706
FaqI GGGAC 2 cut(s) 34, 2237
FatI CATG 9 cut(s) 480, 1408, 1977, 1984, 2138, 2160, 2165, 2298, 2510
FauI CCCGC 1 cut(s) 113
FauNDI CATATG 1 cut(s) 2477
FbaI TGATCA 2 cut(s) 703, 2803
FblI GTMKAC 3 cut(s) 387, 1099, 1619
FokI GGATG 4 cut(s) 649, 1410, 1564, 2442
FriOI GRGCYC 1 cut(s) 575
FspBI CTAG 6 cut(s) 1623, 1901, 2004, 2040, 2118, 2238
GsaI CCCAGC 1 cut(s) 2625
GsuI CTGGAG 4 cut(s) 22, 1227, 2601, 2658
HaeIII GGCC 7 cut(s) 346, 380, 1587, 1736, 2343, 2586, 2626
HapII CCGG 1 cut(s) 87
HgaI GACGC 5 cut(s) 36, 224, 418, 1084, 2324
Hin1I GRCGYC 1 cut(s) 410
Hin1II CATG 9 cut(s) 484, 1412, 1981, 1988, 2142, 2164, 2169, 2302, 2514
HincII GTYRAC 6 cut(s) 239, 388, 1558, 1636, 2296, 2314
HindII GTYRAC 6 cut(s) 239, 388, 1558, 1636, 2296, 2314
HindIII AAGCTT 2 cut(s) 694, 1883
HpaII CCGG 1 cut(s) 87
HphI GGTGA 9 cut(s) 101, 109, 391, 1034, 1316, 1343, 2227, 2313, 2513
Hpy188I TCNGA 8 cut(s) 232, 1204, 1532, 1774, 1945, 1951, 2265, 2803
Hpy99I CGWCG 8 cut(s) 102, 140, 182, 207, 274, 371, 412, 580
HpyAV CCTTC 7 cut(s) 160, 872, 1148, 1344, 1348, 2037, 2397
HpyCH4III ACNGT 4 cut(s) 121, 505, 1231, 2025
HpyCH4IV ACGT 3 cut(s) 155, 2495, 2609
HpyF10VI GCNNNNNNNGC 3 cut(s) 1378, 1649, 1826
HpyF3I CTNAG 5 cut(s) 722, 899, 923, 1881, 2789
HpySE526I ACGT 3 cut(s) 155, 2495, 2609
Hsp92I GRCGYC 1 cut(s) 410
Hsp92II CATG 9 cut(s) 484, 1412, 1981, 1988, 2142, 2164, 2169, 2302, 2514
Ksp22I TGATCA 2 cut(s) 703, 2803
Kzo9I GATC 6 cut(s) 703, 1532, 2303, 2366, 2752, 2803
LguI GCTCTTC 2 cut(s) 1902, 2664
LmnI GCTCC 4 cut(s) 152, 1005, 1222, 2639
Lsp1109I GCAGC 8 cut(s) 56, 345, 532, 904, 1393, 1429, 1801, 1804
LweI GCATC 5 cut(s) 205, 627, 1429, 2327, 2420
MaeI CTAG 6 cut(s) 1623, 1901, 2004, 2040, 2118, 2238
MaeII ACGT 3 cut(s) 155, 2495, 2609
MalI GATC 6 cut(s) 705, 1534, 2305, 2368, 2754, 2805
MbiI CCGCTC 1 cut(s) 147
MboI GATC 6 cut(s) 703, 1532, 2303, 2366, 2752, 2803
MboII GAAGA 8 cut(s) 1409, 1524, 1527, 1775, 1889, 2291, 2455, 2651
MfeI CAATTG 2 cut(s) 436, 1092
MflI RGATCY 3 cut(s) 1532, 2303, 2752
MhlI GDGCHC 4 cut(s) 297, 575, 1010, 2787
MlsI TGGCCA 2 cut(s) 346, 1587
MluNI TGGCCA 2 cut(s) 346, 1587
MlyI GAGTC 2 cut(s) 425, 503
MmeI TCCRAC 5 cut(s) 148, 1529, 1895, 2407, 2462
Mox20I TGGCCA 2 cut(s) 346, 1587
Mph1103I ATGCAT 2 cut(s) 1414, 2162
MroXI GAANNNNTTC 2 cut(s) 785, 1608
MscI TGGCCA 2 cut(s) 346, 1587
MseI TTAA 9 cut(s) 167, 774, 1368, 1502, 1728, 1751, 2013, 2394, 2748
MslI CAYNNNNRTG 4 cut(s) 260, 1013, 1329, 1428
Msp20I TGGCCA 2 cut(s) 346, 1587
MspI CCGG 1 cut(s) 87
MspR9I CCNGG 5 cut(s) 343, 1584, 2212, 2345, 2456
MunI CAATTG 2 cut(s) 436, 1092
MvaI CCWGG 5 cut(s) 343, 1584, 2212, 2345, 2456
MwoI GCNNNNNNNGC 3 cut(s) 1378, 1649, 1826
NcoI CCATGG 2 cut(s) 480, 2138
NdeI CATATG 1 cut(s) 2477
NdeII GATC 6 cut(s) 703, 1532, 2303, 2366, 2752, 2803
NlaIII CATG 9 cut(s) 484, 1412, 1981, 1988, 2142, 2164, 2169, 2302, 2514
NlaIV GGNNCC 4 cut(s) 336, 2305, 2641, 2754
NmeAIII GCCGAG 1 cut(s) 231
NmuCI GTSAC 4 cut(s) 89, 115, 499, 1331
NsiI ATGCAT 2 cut(s) 1414, 2162
NspI RCATGY 3 cut(s) 1412, 1981, 2169
OliI CACNNNNGTG 1 cut(s) 1013
PaqCI CACCTGC 2 cut(s) 410, 2169
PceI AGGCCT 1 cut(s) 1736
PciI ACATGT 2 cut(s) 1977, 2165
PciSI GCTCTTC 2 cut(s) 1902, 2664
PdmI GAANNNNTTC 2 cut(s) 785, 1608
PflMI CCANNNNNTGG 3 cut(s) 659, 2129, 2516
PfoI TCCNGGA 1 cut(s) 2454
PleI GAGTC 2 cut(s) 425, 502
PpsI GAGTC 2 cut(s) 425, 502
Ppu21I YACGTR 1 cut(s) 156
PpuMI RGGWCCY 1 cut(s) 981
PscI ACATGT 2 cut(s) 1977, 2165
PshAI GACNNNNGTC 1 cut(s) 96
PsiI TTATAA 2 cut(s) 1496, 1598
Psp5II RGGWCCY 1 cut(s) 981
Psp6I CCWGG 5 cut(s) 341, 1582, 2210, 2343, 2454
PspEI GGTNACC 2 cut(s) 89, 2406
PspFI CCCAGC 1 cut(s) 2621
PspGI CCWGG 5 cut(s) 341, 1582, 2210, 2343, 2454
PspN4I GGNNCC 4 cut(s) 336, 2305, 2641, 2754
PspPI GGNCC 4 cut(s) 334, 981, 1357, 2624
PspPPI RGGWCCY 1 cut(s) 981
PstI CTGCAG 1 cut(s) 810
PstNI CAGNNNCTG 2 cut(s) 358, 1250
PsuI RGATCY 3 cut(s) 1532, 2303, 2752
RsaI GTAC 5 cut(s) 103, 563, 1030, 1146, 2116
RsaNI GTAC 5 cut(s) 102, 562, 1029, 1145, 2115
RseI CAYNNNNRTG 4 cut(s) 260, 1013, 1329, 1428
SalI GTCGAC 1 cut(s) 386
SapI GCTCTTC 2 cut(s) 1902, 2664
SaqAI TTAA 9 cut(s) 167, 774, 1368, 1502, 1728, 1751, 2013, 2394, 2748
Sau3AI GATC 6 cut(s) 703, 1532, 2303, 2366, 2752, 2803
Sau96I GGNCC 4 cut(s) 334, 981, 1357, 2624
ScaI AGTACT 1 cut(s) 2116
SchI GAGTC 2 cut(s) 425, 503
ScrFI CCNGG 5 cut(s) 343, 1584, 2212, 2345, 2456
SduI GDGCHC 4 cut(s) 297, 575, 1010, 2787
SfaNI GCATC 5 cut(s) 205, 627, 1429, 2327, 2420
SfcI CTRYAG 2 cut(s) 806, 1104
SinI GGWCC 3 cut(s) 334, 981, 1357
SmiMI CAYNNNNRTG 4 cut(s) 260, 1013, 1329, 1428
SpeI ACTAGT 2 cut(s) 1622, 2117
SseBI AGGCCT 1 cut(s) 1736
SspI AATATT 1 cut(s) 1527
SspMI CTAG 6 cut(s) 1623, 1901, 2004, 2040, 2118, 2238
StuI AGGCCT 1 cut(s) 1736
StyD4I CCNGG 5 cut(s) 341, 1582, 2210, 2343, 2454
StyI CCWWGG 3 cut(s) 480, 977, 2138
TaaI ACNGT 4 cut(s) 121, 505, 1231, 2025
TaiI ACGT 3 cut(s) 158, 2498, 2612
TaqI TCGA 8 cut(s) 180, 387, 575, 798, 880, 2709, 2715, 2778
TatI WGTACW 4 cut(s) 561, 1028, 1144, 2114
TauI GCSGC 4 cut(s) 268, 315, 930, 2237
Tru1I TTAA 9 cut(s) 167, 774, 1368, 1502, 1728, 1751, 2013, 2394, 2748
Tru9I TTAA 9 cut(s) 167, 774, 1368, 1502, 1728, 1751, 2013, 2394, 2748
TscAI CASTG 7 cut(s) 510, 610, 660, 852, 912, 1234, 1575
TseFI GTSAC 4 cut(s) 89, 115, 499, 1331
TseI GCWGC 8 cut(s) 44, 358, 520, 892, 1381, 1442, 1814, 1817
Tsp45I GTSAC 4 cut(s) 89, 115, 499, 1331
TspGWI ACGGA 6 cut(s) 27, 103, 191, 280, 355, 1025
TspRI CASTG 7 cut(s) 510, 610, 660, 852, 912, 1234, 1575
Van91I CCANNNNNTGG 3 cut(s) 659, 2129, 2516
VpaK11BI GGWCC 3 cut(s) 334, 981, 1357
XagI CCTNNNNNAGG 1 cut(s) 873
XapI RAATTY 6 cut(s) 246, 451, 716, 910, 1161, 1804
XbaI TCTAGA 1 cut(s) 1900
XceI RCATGY 3 cut(s) 1412, 1981, 2169
XcmI CCANNNNNNNNNTGG 1 cut(s) 1688
XmiI GTMKAC 3 cut(s) 387, 1099, 1619
XmnI GAANNNNTTC 2 cut(s) 785, 1608
XspI CTAG 6 cut(s) 1623, 1901, 2004, 2040, 2118, 2238
ZrmI AGTACT 1 cut(s) 2116
Zsp2I ATGCAT 2 cut(s) 1414, 2162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.