Rw1G012450

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0001590
Physical Location & Seq
Reverse (-)
27683955 .. 27693021
9067 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G012450.1

Sequence Viewer

Length: 597 bp
ATGTGGGTGCATGAATTAGAAGTGCCAGTATTTGGGTCTCTTGCCATCAATTCTCTGAATGGAAATATTATATGTTGCTTGGTGGATGGGCATGTTCTTGCATTTACTACAAGTGGATCTATTATTTGGAGGTATAGAACTGCTGGGCCTGTATTTGCTGGAGCCTGCATATCTTCTGCTCTTCCCTTTCAGCTACTTGTCTGTTCCAGAGATGGAAACATCTATTCACTCGAACTCGAATCGGGAGGTTTACTCTGGGAATACAATGTTAAGGATCCAATTACTTCATCTGCCTATGTCGATGAGCACCTGAGCTTGGTGTCTGATCAGTCTAACTTTCCCGACCGCAGGGTGGTTTGTGTATGTTCCAGCTCCGGAAGCATGGTTCTGATTCAGGTTAATTTGGATGCCACCGAAGGTGCAAGTCCAATGAATTTCAATGTTGAGGAATTTGCAAGGTTGGAACTCCCAGGAGATGTATTCTCTTCCCCTGTAATGATTGGTGGCACAATTTTCGTTGGTTGTAGGGATGATTATGTGCACTGCATTGCTGTTAAGCCCCAGGTCTCTACAGGAACAATGAGTTGTTCTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C PP-binding PQQ PQQ_2 PQQ_3

Protein Analysis

198

Amino Acids

21.32

Weight (kDa)

4.53

Isoelectric Point (pI)

39.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_ACSF4 PF13570 1 - 184 2.1e-39 Beta-alanine-activating enzyme, beta-propeller
PQQ_2 PF13360 5 - 185 2.4e-09 Outer membrane protein assembly factor BamB
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 32
AccIII TCCGGA 1 cut(s) 374
AciI CCGC 1 cut(s) 346
AclWI GGATC 3 cut(s) 124, 269, 282
AcsI RAATTY 2 cut(s) 433, 449
AfiI CCNNNNNNNGG 4 cut(s) 32, 316, 348, 352
AgsI TTSAA 1 cut(s) 439
AjnI CCWGG 2 cut(s) 469, 561
AloI GAACNNNNNNTCC 2 cut(s) 369, 401
AluBI AGCT 3 cut(s) 193, 315, 372
AluI AGCT 3 cut(s) 193, 315, 372
Alw21I GWGCWC 2 cut(s) 309, 543
Alw26I GTCTC 2 cut(s) 42, 571
Alw44I GTGCAC 1 cut(s) 539
AlwI GGATC 3 cut(s) 124, 269, 282
Aor13HI TCCGGA 1 cut(s) 374
AoxI GGCC 1 cut(s) 146
ApaLI GTGCAC 1 cut(s) 539
ApoI RAATTY 2 cut(s) 433, 449
AspS9I GGNCC 1 cut(s) 146
BaeGI GKGCMC 1 cut(s) 543
BamHI GGATCC 1 cut(s) 274
Bbv12I GWGCWC 2 cut(s) 309, 543
BccI CCATC 3 cut(s) 53, 80, 206
BcgI CGANNNNNNTGC 2 cut(s) 496, 530
BciT130I CCWGG 2 cut(s) 471, 563
BclI TGATCA 1 cut(s) 325
BcoDI GTCTC 2 cut(s) 42, 571
BfmI CTRYAG 1 cut(s) 570
Bme1390I CCNGG 2 cut(s) 471, 563
BmgT120I GGNCC 1 cut(s) 146
BmiI GGNNCC 2 cut(s) 163, 276
BmrFI CCNGG 2 cut(s) 471, 563
BmsI GCATC 1 cut(s) 397
BplI GAGNNNNNCTC 2 cut(s) 237, 269
BpmI CTGGAG 1 cut(s) 180
Bpu10I CCTNAGC 1 cut(s) 311
BsaI GGTCTC 2 cut(s) 42, 571
BsaJI CCNNGG 2 cut(s) 469, 561
BsaWI WCCGGW 1 cut(s) 374
Bsc4I CCNNNNNNNGG 4 cut(s) 32, 316, 348, 352
Bse1I ACTGG 1 cut(s) 26
Bse3DI GCAATG 1 cut(s) 546
BseAI TCCGGA 1 cut(s) 374
BseBI CCWGG 2 cut(s) 471, 563
BseDI CCNNGG 2 cut(s) 469, 561
BseGI GGATG 3 cut(s) 91, 412, 535
BseLI CCNNNNNNNGG 4 cut(s) 32, 316, 348, 352
BseMI GCAATG 1 cut(s) 546
BseMII CTCAG 1 cut(s) 302
BseNI ACTGG 1 cut(s) 26
BseSI GKGCMC 1 cut(s) 543
BseYI CCCAGC 1 cut(s) 143
Bsh1285I CGRYCG 1 cut(s) 346
BshFI GGCC 1 cut(s) 148
BsiEI CGRYCG 1 cut(s) 346
BsiHKAI GWGCWC 2 cut(s) 309, 543
BsiSI CCGG 1 cut(s) 375
BslI CCNNNNNNNGG 4 cut(s) 32, 316, 348, 352
BsmAI GTCTC 2 cut(s) 42, 571
BsnI GGCC 1 cut(s) 148
Bso31I GGTCTC 2 cut(s) 42, 571
Bsp1286I GDGCHC 2 cut(s) 309, 543
Bsp13I TCCGGA 1 cut(s) 374
Bsp143I GATC 3 cut(s) 116, 274, 325
BspACI CCGC 1 cut(s) 346
BspANI GGCC 1 cut(s) 148
BspCNI CTCAG 1 cut(s) 303
BspEI TCCGGA 1 cut(s) 374
BspLI GGNNCC 2 cut(s) 163, 276
BspPI GGATC 3 cut(s) 124, 269, 282
BspQI GCTCTTC 1 cut(s) 186
BspTNI GGTCTC 2 cut(s) 42, 571
BsrDI GCAATG 1 cut(s) 546
BsrI ACTGG 1 cut(s) 26
BssECI CCNNGG 2 cut(s) 469, 561
BssMI GATC 3 cut(s) 116, 274, 325
Bst2UI CCWGG 2 cut(s) 471, 563
Bst6I CTCTTC 2 cut(s) 186, 490
BstC8I GCNNGC 1 cut(s) 166
BstDEI CTNAG 1 cut(s) 311
BstF5I GGATG 3 cut(s) 91, 412, 535
BstKTI GATC 3 cut(s) 119, 277, 328
BstMAI GTCTC 2 cut(s) 42, 571
BstMBI GATC 3 cut(s) 116, 274, 325
BstMCI CGRYCG 1 cut(s) 346
BstMWI GCNNNNNNNGC 1 cut(s) 378
BstNI CCWGG 2 cut(s) 471, 563
BstNSI RCATGY 1 cut(s) 95
BstSCI CCNGG 2 cut(s) 469, 561
BstSFI CTRYAG 1 cut(s) 570
BstSLI GKGCMC 1 cut(s) 543
BstX2I RGATCY 2 cut(s) 116, 274
BstYI RGATCY 2 cut(s) 116, 274
BsuRI GGCC 1 cut(s) 148
BtsCI GGATG 3 cut(s) 91, 412, 535
BtsI GCAGTG 1 cut(s) 541
BtsIMutI CAGTG 1 cut(s) 541
Cac8I GCNNGC 1 cut(s) 166
Cfr13I GGNCC 1 cut(s) 146
CviAII CATG 3 cut(s) 11, 92, 382
CviJI RGCY 6 cut(s) 148, 164, 193, 315, 372, 559
CviKI_1 RGCY 6 cut(s) 148, 164, 193, 315, 372, 559
DdeI CTNAG 1 cut(s) 311
DpnI GATC 3 cut(s) 118, 276, 327
DpnII GATC 3 cut(s) 116, 274, 325
Eam1104I CTCTTC 2 cut(s) 186, 490
EarI CTCTTC 2 cut(s) 186, 490
Eco31I GGTCTC 2 cut(s) 42, 571
EcoRII CCWGG 2 cut(s) 469, 561
FaeI CATG 3 cut(s) 14, 95, 385
FatI CATG 3 cut(s) 10, 91, 381
FbaI TGATCA 1 cut(s) 325
FokI GGATG 3 cut(s) 98, 419, 542
GsaI CCCAGC 1 cut(s) 147
GsuI CTGGAG 1 cut(s) 180
HaeIII GGCC 1 cut(s) 148
HapII CCGG 1 cut(s) 375
Hin1II CATG 3 cut(s) 14, 95, 385
HinfI GANTC 2 cut(s) 239, 391
HpaII CCGG 1 cut(s) 375
Hpy166II GTNNAC 2 cut(s) 251, 541
Hpy188I TCNGA 3 cut(s) 57, 325, 390
Hpy188III TCNNGA 4 cut(s) 207, 243, 341, 375
Hpy8I GTNNAC 2 cut(s) 251, 541
HpyAV CCTTC 1 cut(s) 410
HpyCH4V TGCA 7 cut(s) 10, 101, 168, 422, 455, 541, 546
HpyF10VI GCNNNNNNNGC 1 cut(s) 378
HpyF3I CTNAG 1 cut(s) 311
Hsp92II CATG 3 cut(s) 14, 95, 385
Kpn2I TCCGGA 1 cut(s) 374
Ksp22I TGATCA 1 cut(s) 325
Kzo9I GATC 3 cut(s) 116, 274, 325
LguI GCTCTTC 1 cut(s) 186
LmnI GCTCC 2 cut(s) 161, 377
LweI GCATC 1 cut(s) 397
MalI GATC 3 cut(s) 118, 276, 327
MboI GATC 3 cut(s) 116, 274, 325
MboII GAAGA 3 cut(s) 165, 173, 477
MflI RGATCY 2 cut(s) 116, 274
MhlI GDGCHC 2 cut(s) 309, 543
MluCI AATT 7 cut(s) 14, 49, 279, 400, 433, 449, 510
MmeI TCCRAC 1 cut(s) 441
MnlI CCTC 3 cut(s) 123, 239, 439
MroI TCCGGA 1 cut(s) 374
MseI TTAA 3 cut(s) 270, 399, 555
MspI CCGG 1 cut(s) 375
MspR9I CCNGG 2 cut(s) 471, 563
MvaI CCWGG 2 cut(s) 471, 563
MwoI GCNNNNNNNGC 1 cut(s) 378
NdeII GATC 3 cut(s) 116, 274, 325
NlaIII CATG 3 cut(s) 14, 95, 385
NlaIV GGNNCC 2 cut(s) 163, 276
NspI RCATGY 1 cut(s) 95
PciSI GCTCTTC 1 cut(s) 186
PfeI GAWTC 2 cut(s) 239, 391
PflMI CCANNNNNTGG 1 cut(s) 32
Psp6I CCWGG 2 cut(s) 469, 561
PspFI CCCAGC 1 cut(s) 143
PspGI CCWGG 2 cut(s) 469, 561
PspN4I GGNNCC 2 cut(s) 163, 276
PspPI GGNCC 1 cut(s) 146
PsuI RGATCY 2 cut(s) 116, 274
SapI GCTCTTC 1 cut(s) 186
SaqAI TTAA 3 cut(s) 270, 399, 555
Sau3AI GATC 3 cut(s) 116, 274, 325
Sau96I GGNCC 1 cut(s) 146
ScrFI CCNGG 2 cut(s) 471, 563
SduI GDGCHC 2 cut(s) 309, 543
SfaNI GCATC 1 cut(s) 397
SfcI CTRYAG 1 cut(s) 570
Sse9I AATT 7 cut(s) 14, 49, 279, 400, 433, 449, 510
SsiI CCGC 1 cut(s) 346
SspI AATATT 1 cut(s) 67
StyD4I CCNGG 2 cut(s) 469, 561
TaqI TCGA 3 cut(s) 231, 237, 300
TasI AATT 7 cut(s) 14, 49, 279, 400, 433, 449, 510
TfiI GAWTC 2 cut(s) 239, 391
Tru1I TTAA 3 cut(s) 270, 399, 555
Tru9I TTAA 3 cut(s) 270, 399, 555
TscAI CASTG 1 cut(s) 548
TspDTI ATGAA 3 cut(s) 27, 276, 446
TspRI CASTG 1 cut(s) 548
Van91I CCANNNNNTGG 1 cut(s) 32
VneI GTGCAC 1 cut(s) 539
XapI RAATTY 2 cut(s) 433, 449
XceI RCATGY 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.