Rh1DG159600

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0001590
Physical Location & Seq
Reverse (-)
32918413 .. 32925844
7432 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG159600.1

Sequence Viewer

Length: 2403 bp
ATGTCGACCTCATTATCACCTGCACGACGCCGTTTGGTTACGAATTGGACTCCAATTGGCTGGAAAAGAATTCCAATCGTAGCTTTCTGTGGTTTTCCATGGGTGGTGGAGTCTGTGACAGTGAGTGATGGAGAGCAGCGTAAGAGTGAGAAAGAGAGGCTGTTCTGTTATGTAATGTACACTTCGGGCTCGACAGGGAAGCCGAAAGGTGTCTGTGGAACTGAACAAGGTCTTTGGAATCGGTTTCGGTGGATGCAAGAATTGTATCCATTGAATGGAGAGGAGGTTTTGTTGTTCAAGACAGCAATAAGCTTTGTTGATCACTTGCAAGAATTTCTTAGTGCTATTCTTACTGGTTCTACTTTGGTCATACCTCCTTTCAATCAACTTAAACAGAATGTGTTCTCCATTCTCGATTTTCTGCAGGCTTATTCTATCAATAGACTTACTGCTGTTCCATCACTGATGAGAGTAATCCTTCCAGCTTTGCAAGGTCGACACTACCAGCAGCTTCTAAGTTCACTGAATTTGTTAGTGCTAAGCGGTGAAGTTTTGCCTTTATCCTTGTGGGATATGCTGTCAAAGAGATTACCAAGGACCTCTATTTTGAATCTGTATGGGAGCACGGAGGTGTCAGGTGATTGTACATATTTTGACTGCAAGAGGTTGCCAATGATTTTGGAGACAGAGACGCTAACAAGTGTTCCAATTGGTATACCTATAGCTGGTTGTGATGTTGTGCTTGTTAATGATGATGATGTACTCAATGAAGGAGAAATTTTTGTTGCTGGTCCCTGCAATGCTTCTGGATACTATTCTGATTCTACTTTTGCTCCTTTGAACACTGTAAAATTACCTCCAGATTCTGTTTGTTCCAGTTCTGTAAATGGACACGAAAGTCAATCATATTTTCAAACTGGTGATTTCGCCAAACAACTTCATAGCGGTGACTTGGTTTTCTTGGGAAGGAAGAACCGCACTATTAAGCTCAATGGGCAGCGTATTGCTTTAGAAGAAATTGAACATTCATTGGTGGAACATCCAGATGTAACTGATGCTGCTGTTGTTTTTCGTAATGGTCAAGGGGAACTTATGCAACTTATAGCATTTATAATGTTAAAGGAGGGAAGACCTGATGAAATATTCAGATCTTCCATCAAAAGTTGGATGGTTGACAAACTTCCACTGGCAATGATTCCTGGGCACTTTGTTATAATGGAATCATTTCCTGTGTCTACTAGTGGAAAAGTTGACTATGCTTTGTTAGCAGATTCAGTATTTCTGGCAAAACATATCCAAGATGAGCTTGGTCATATTGGGAGAAGCAATCTACTGCAAGTAATTAAAAAGGCCTTTAGTCGTGTTTTAATGATGGCTGAAGAGGTTTCCGATGATGATGATTTCTTTATGATTGGTGGAGATTCTATTGCTGCTGCACATCTTGCTAACAATATAGGAGTTGATATGAGATTGATATATAGCTTTCCAACTCCATCTAAGCTTTCTATGGCTCTTCTAGAGAGAAAAGAACCATTCAGTATGAAAGTTGAAGTGGATTCTGAATCCGAGATGAATCAGGAAAGGGGAAAGAACATGTTTCATGTCCTCTATGACACTCCTAGTGCTGTTAATCTTGAACAGTTGAAAACTCTGCCTAGAAGGAATGAAAATAAAGCAGTTATGTCTAAACGCTTGAAAGCCGATTCAAGTATAAATGTTGCTTCAGGGAGTACTAGTCCAGCAGATGGGTATCCATGGAGTTCTATGCAAAAATGCATGACATGTTCTTTCAGCAGGTGCAACAAAGTTGTGTATGAGGGCAGTTCCAGGGTGAATGATGTTTGTCAAGCGGCTAGTTCTGTGGTTGTCCCAAAAAGTCTAAAAGTGCATTTGGAAGAATTGTGGAAAGTTGACATGGGATCCTGCGTTGACGCATCACCACTTATTGTTTGTAAAGGCCAGGATATCTACTTATTTATTGGATCACACTCACAAAAGTTTGTGTGTGTTAATGCCAGAAGTGGTTCCATCCAATGGGAGTTACAACTTGAAGGACGTGTAGAATGTTCAGCAGCAATTCTTGGTGACTTTACTCAGGTTGTAGTTGGATGCTACAAAGGGAAGATATATTTCCTGGATTCTTTGAATGGTAACATATGCTGGACTTTCCAAACATCTGGTGAGGTAAAGTCACAGCCGATTATAGACAGTCAAAGACAATTGATCTGGTGTGGGTCATATGACCGTAACTTGTATGTGCTAGATTATAAAAATCATTGCTGTGTTTATCAGGTTCCCTGTGGTGGGAGTATCTGTGGGTCTCCTGCAATAGATGAGATGAATAATATACTTTATGTGGCTTCTACTGGTGGCCAAATGACAGCCATTTCAGTAACGGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C PP-binding PQQ PQQ_2 PQQ_3

Protein Analysis

800

Amino Acids

88.74

Weight (kDa)

5.79

Isoelectric Point (pI)

43.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 46 - 272 1.4e-39 AMP-binding enzyme
AMP-binding_C PF13193 339 - 416 2.9e-09 AMP-binding enzyme C-terminal domain
PP-binding PF00550 447 - 501 4.3e-06 Phosphopantetheine attachment site
PQQ_2 PF13360 632 - 736 2.5e-08 Outer membrane protein assembly factor BamB
Beta-prop_ACSF4 PF13570 639 - 797 9.7e-57 Beta-alanine-activating enzyme, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1112, 1214, 2268
AarI CACCTGC 2 cut(s) 28, 1785
AasI GACNNNNNNGTC 1 cut(s) 897
Acc36I ACCTGC 2 cut(s) 28, 1785
AccB7I CCANNNNNTGG 3 cut(s) 275, 1745, 2034
AccI GTMKAC 4 cut(s) 5, 496, 715, 1235
AciI CCGC 4 cut(s) 543, 945, 976, 1850
AclWI GGATC 3 cut(s) 1914, 1927, 1990
AcoI YGGCCR 1 cut(s) 2371
AcsI RAATTY 4 cut(s) 69, 332, 526, 777
AcuI CTGAAG 2 cut(s) 1398, 1707
AcyI GRCGYC 1 cut(s) 28
AfaI GTAC 4 cut(s) 179, 646, 762, 1732
AfiI CCNNNNNNNGG 6 cut(s) 275, 725, 1745, 2034, 2303, 2304
AflIII ACRYGT 3 cut(s) 1593, 1781, 2056
AhlI ACTAGT 2 cut(s) 1238, 1733
AjiI CACGTC 1 cut(s) 2057
AjnI CCWGG 4 cut(s) 1198, 1826, 1959, 2133
AleI CACNNNNGTG 1 cut(s) 629
AluBI AGCT 9 cut(s) 83, 312, 485, 511, 725, 988, 1306, 1482, 1501
AluI AGCT 9 cut(s) 83, 312, 485, 511, 725, 988, 1306, 1482, 1501
Alw21I GWGCWC 1 cut(s) 626
Alw26I GTCTC 3 cut(s) 677, 683, 2325
AlwI GGATC 3 cut(s) 1914, 1927, 1990
AlwNI CAGNNNCTG 1 cut(s) 866
AoxI GGCC 3 cut(s) 1350, 1957, 2371
ApeKI GCWGC 7 cut(s) 136, 508, 997, 1058, 1430, 1433, 2072
ApoI RAATTY 4 cut(s) 69, 332, 526, 777
ArsI GACNNNNNNTTYG 2 cut(s) 589, 621
Asp700I GAANNNNTTC 3 cut(s) 401, 1224, 2023
AspS9I GGNCC 2 cut(s) 597, 791
AsuHPI GGTGA 9 cut(s) 9, 557, 650, 932, 959, 1843, 1929, 2096, 2192
AvaII GGWCC 2 cut(s) 597, 791
BaeGI GKGCMC 1 cut(s) 1206
BalI TGGCCA 1 cut(s) 2373
BamHI GGATCC 1 cut(s) 1919
BanII GRGCYC 1 cut(s) 191
BbsI GAAGAC 1 cut(s) 1135
Bbv12I GWGCWC 1 cut(s) 626
BbvI GCAGC 7 cut(s) 148, 520, 1009, 1045, 1417, 1420, 2084
BccI CCATC 8 cut(s) 122, 466, 1162, 1163, 1366, 1501, 1739, 2036
BceAI ACGGC 1 cut(s) 15
BciT130I CCWGG 4 cut(s) 1200, 1828, 1961, 2135
BciVI GTATCC 3 cut(s) 276, 803, 1761
BclI TGATCA 1 cut(s) 319
BcoDI GTCTC 3 cut(s) 677, 683, 2325
BcuI ACTAGT 2 cut(s) 1238, 1733
BfaI CTAG 7 cut(s) 1239, 1517, 1620, 1656, 1734, 1854, 2261
BfmI CTRYAG 2 cut(s) 422, 720
BfuAI ACCTGC 2 cut(s) 28, 1785
BfuI GTATCC 3 cut(s) 276, 803, 1761
BglII AGATCT 1 cut(s) 1148
BisI GCNGC 8 cut(s) 137, 509, 998, 1059, 1431, 1434, 1851, 2073
BlpI GCTNAGC 1 cut(s) 539
BlsI GCNGC 8 cut(s) 138, 510, 999, 1060, 1432, 1435, 1852, 2074
BmcAI AGTACT 1 cut(s) 1732
Bme1390I CCNGG 4 cut(s) 1200, 1828, 1961, 2135
Bme18I GGWCC 2 cut(s) 597, 791
BmgBI CACGTC 1 cut(s) 2057
BmgT120I GGNCC 2 cut(s) 597, 791
BmiI GGNNCC 4 cut(s) 793, 1921, 2026, 2295
BmrFI CCNGG 4 cut(s) 1200, 1828, 1961, 2135
BmsI GCATC 4 cut(s) 243, 1045, 1943, 2099
BpiI GAAGAC 1 cut(s) 1135
BpmI CTGGAG 1 cut(s) 843
Bpu1102I GCTNAGC 1 cut(s) 539
BsaBI GATNNNNATC 1 cut(s) 1749
BsaHI GRCGYC 1 cut(s) 28
BsaI GGTCTC 1 cut(s) 2325
BsaJI CCNNGG 5 cut(s) 98, 593, 1199, 1754, 1827
Bsc4I CCNNNNNNNGG 6 cut(s) 275, 725, 1745, 2034, 2303, 2304
Bse1I ACTGG 5 cut(s) 358, 876, 922, 1191, 2371
Bse3DI GCAATG 3 cut(s) 805, 1197, 2275
Bse8I GATNNNNATC 1 cut(s) 1749
BseBI CCWGG 4 cut(s) 1200, 1828, 1961, 2135
BseDI CCNNGG 5 cut(s) 98, 593, 1199, 1754, 1827
BseGI GGATG 5 cut(s) 258, 1039, 1173, 2028, 2114
BseJI GATNNNNATC 1 cut(s) 1749
BseLI CCNNNNNNNGG 6 cut(s) 275, 725, 1745, 2034, 2303, 2304
BseMI GCAATG 3 cut(s) 805, 1197, 2275
BseMII CTCAG 1 cut(s) 2108
BseNI ACTGG 5 cut(s) 358, 876, 922, 1191, 2371
BseRI GAGGAG 1 cut(s) 296
BseSI GKGCMC 1 cut(s) 1206
BseXI GCAGC 7 cut(s) 148, 520, 1009, 1045, 1417, 1420, 2084
BsgI GTGCAG 2 cut(s) 6, 1419
BshFI GGCC 3 cut(s) 1352, 1959, 2373
BsiHKAI GWGCWC 1 cut(s) 626
BslFI GGGAC 2 cut(s) 777, 1853
BslI CCNNNNNNNGG 6 cut(s) 275, 725, 1745, 2034, 2303, 2304
BsmAI GTCTC 3 cut(s) 677, 683, 2325
BsmBI CGTCTC 1 cut(s) 683
BsmFI GGGAC 2 cut(s) 777, 1853
BsnI GGCC 3 cut(s) 1352, 1959, 2373
Bso31I GGTCTC 1 cut(s) 2325
Bsp1286I GDGCHC 3 cut(s) 191, 626, 1206
Bsp1407I TGTACA 2 cut(s) 177, 644
Bsp143I GATC 5 cut(s) 319, 1148, 1919, 1982, 2223
Bsp1720I GCTNAGC 1 cut(s) 539
Bsp19I CCATGG 2 cut(s) 98, 1754
BspACI CCGC 4 cut(s) 543, 945, 976, 1850
BspANI GGCC 3 cut(s) 1352, 1959, 2373
BspCNI CTCAG 1 cut(s) 2107
BspLI GGNNCC 4 cut(s) 793, 1921, 2026, 2295
BspMAI CTGCAG 1 cut(s) 426
BspMI ACCTGC 2 cut(s) 28, 1785
BspPI GGATC 3 cut(s) 1914, 1927, 1990
BspQI GCTCTTC 1 cut(s) 1518
BspTNI GGTCTC 1 cut(s) 2325
BsrDI GCAATG 3 cut(s) 805, 1197, 2275
BsrGI TGTACA 2 cut(s) 177, 644
BsrI ACTGG 5 cut(s) 358, 876, 922, 1191, 2371
BssECI CCNNGG 5 cut(s) 98, 593, 1199, 1754, 1827
BssMI GATC 5 cut(s) 319, 1148, 1919, 1982, 2223
BssNAI GTATAC 1 cut(s) 716
BssNI GRCGYC 1 cut(s) 28
BssT1I CCWWGG 3 cut(s) 98, 593, 1754
Bst1107I GTATAC 1 cut(s) 716
Bst2UI CCWGG 4 cut(s) 1200, 1828, 1961, 2135
Bst4CI ACNGT 6 cut(s) 121, 847, 1641, 2210, 2246, 2398
Bst6I CTCTTC 2 cut(s) 1374, 1518
BstACI GRCGYC 1 cut(s) 28
BstAPI GCANNNNNTGC 1 cut(s) 1442
BstAUI TGTACA 2 cut(s) 177, 644
BstC8I GCNNGC 1 cut(s) 426
BstDEI CTNAG 5 cut(s) 338, 515, 539, 1497, 2094
BstDSI CCRYGG 2 cut(s) 98, 1754
BstF5I GGATG 5 cut(s) 258, 1039, 1173, 2028, 2114
BstKTI GATC 5 cut(s) 322, 1151, 1922, 1985, 2226
BstMAI GTCTC 3 cut(s) 677, 683, 2325
BstMBI GATC 5 cut(s) 319, 1148, 1919, 1982, 2223
BstMWI GCNNNNNNNGC 3 cut(s) 994, 1265, 1442
BstNI CCWGG 4 cut(s) 1200, 1828, 1961, 2135
BstNSI RCATGY 2 cut(s) 1597, 1785
BstSCI CCNGG 4 cut(s) 1198, 1826, 1959, 2133
BstSFI CTRYAG 2 cut(s) 422, 720
BstSLI GKGCMC 1 cut(s) 1206
BstV1I GCAGC 7 cut(s) 148, 520, 1009, 1045, 1417, 1420, 2084
BstV2I GAAGAC 1 cut(s) 1135
BstX2I RGATCY 2 cut(s) 1148, 1919
BstXI CCANNNNNNTGG 2 cut(s) 60, 2177
BstYI RGATCY 2 cut(s) 1148, 1919
BstZ17I GTATAC 1 cut(s) 716
BsuI GTATCC 3 cut(s) 276, 803, 1761
BsuRI GGCC 3 cut(s) 1352, 1959, 2373
BtgI CCRYGG 2 cut(s) 98, 1754
BtrI CACGTC 1 cut(s) 2057
BtsCI GGATG 5 cut(s) 258, 1039, 1173, 2028, 2114
BtsIMutI CAGTG 5 cut(s) 126, 461, 521, 843, 1184
BveI ACCTGC 2 cut(s) 28, 1785
Cac8I GCNNGC 1 cut(s) 426
CaiI CAGNNNCTG 1 cut(s) 866
Cfr13I GGNCC 2 cut(s) 597, 791
CseI GACGC 3 cut(s) 36, 700, 1940
Csp6I GTAC 4 cut(s) 178, 645, 761, 1731
CviAII CATG 7 cut(s) 99, 1594, 1601, 1755, 1777, 1782, 1915
CviQI GTAC 4 cut(s) 178, 645, 761, 1731
DdeI CTNAG 5 cut(s) 338, 515, 539, 1497, 2094
DpnI GATC 5 cut(s) 321, 1150, 1921, 1984, 2225
DpnII GATC 5 cut(s) 319, 1148, 1919, 1982, 2223
DrdI GACNNNNNNGTC 1 cut(s) 897
DseDI GACNNNNNNGTC 1 cut(s) 897
EaeI YGGCCR 1 cut(s) 2371
Eam1104I CTCTTC 2 cut(s) 1374, 1518
EarI CTCTTC 2 cut(s) 1374, 1518
Eco130I CCWWGG 3 cut(s) 98, 593, 1754
Eco147I AGGCCT 1 cut(s) 1352
Eco24I GRGCYC 1 cut(s) 191
Eco31I GGTCTC 1 cut(s) 2325
Eco32I GATATC 1 cut(s) 1966
Eco47I GGWCC 2 cut(s) 597, 791
Eco57I CTGAAG 2 cut(s) 1398, 1707
EcoO109I RGGNCCY 1 cut(s) 597
EcoRI GAATTC 1 cut(s) 69
EcoRII CCWGG 4 cut(s) 1198, 1826, 1959, 2133
EcoRV GATATC 1 cut(s) 1966
EcoT14I CCWWGG 3 cut(s) 98, 593, 1754
EcoT22I ATGCAT 1 cut(s) 1778
EcoT38I GRGCYC 1 cut(s) 191
ErhI CCWWGG 3 cut(s) 98, 593, 1754
Esp3I CGTCTC 1 cut(s) 683
FaeI CATG 7 cut(s) 102, 1597, 1604, 1758, 1780, 1785, 1918
FalI AAGNNNNNCTT 6 cut(s) 321, 353, 1074, 1106, 1290, 1322
FaqI GGGAC 2 cut(s) 777, 1853
FatI CATG 7 cut(s) 98, 1593, 1600, 1754, 1776, 1781, 1914
FauNDI CATATG 2 cut(s) 2156, 2239
FbaI TGATCA 1 cut(s) 319
FblI GTMKAC 4 cut(s) 5, 496, 715, 1235
Fnu4HI GCNGC 8 cut(s) 137, 509, 998, 1059, 1431, 1434, 1851, 2073
FokI GGATG 5 cut(s) 265, 1026, 1180, 2015, 2121
FriOI GRGCYC 1 cut(s) 191
Fsp4HI GCNGC 8 cut(s) 137, 509, 998, 1059, 1431, 1434, 1851, 2073
FspBI CTAG 7 cut(s) 1239, 1517, 1620, 1656, 1734, 1854, 2261
GluI GCNGC 8 cut(s) 137, 509, 998, 1059, 1431, 1434, 1851, 2073
GsuI CTGGAG 1 cut(s) 843
HaeIII GGCC 3 cut(s) 1352, 1959, 2373
HgaI GACGC 3 cut(s) 36, 700, 1940
Hin1I GRCGYC 1 cut(s) 28
Hin1II CATG 7 cut(s) 102, 1597, 1604, 1758, 1780, 1785, 1918
HincII GTYRAC 6 cut(s) 6, 497, 1174, 1252, 1912, 1930
HindII GTYRAC 6 cut(s) 6, 497, 1174, 1252, 1912, 1930
HindIII AAGCTT 2 cut(s) 310, 1499
HphI GGTGA 9 cut(s) 9, 557, 650, 932, 959, 1843, 1929, 2096, 2192
Hpy188I TCNGA 5 cut(s) 820, 1148, 1390, 1561, 1567
Hpy188III TCNNGA 8 cut(s) 298, 413, 807, 860, 1043, 1517, 1577, 1634
Hpy99I CGWCG 1 cut(s) 30
HpyAV CCTTC 5 cut(s) 488, 764, 960, 1653, 2045
HpyCH4III ACNGT 6 cut(s) 121, 847, 1641, 2210, 2246, 2398
HpyCH4IV ACGT 1 cut(s) 2056
HpyF10VI GCNNNNNNNGC 3 cut(s) 994, 1265, 1442
HpyF3I CTNAG 5 cut(s) 338, 515, 539, 1497, 2094
HpySE526I ACGT 1 cut(s) 2056
Hsp92I GRCGYC 1 cut(s) 28
Hsp92II CATG 7 cut(s) 102, 1597, 1604, 1758, 1780, 1785, 1918
Ksp22I TGATCA 1 cut(s) 319
Kzo9I GATC 5 cut(s) 319, 1148, 1919, 1982, 2223
LguI GCTCTTC 1 cut(s) 1518
LmnI GCTCC 2 cut(s) 621, 838
Lsp1109I GCAGC 7 cut(s) 148, 520, 1009, 1045, 1417, 1420, 2084
LweI GCATC 4 cut(s) 243, 1045, 1943, 2099
MaeI CTAG 7 cut(s) 1239, 1517, 1620, 1656, 1734, 1854, 2261
MaeII ACGT 1 cut(s) 2056
MalI GATC 5 cut(s) 321, 1150, 1921, 1984, 2225
MboI GATC 5 cut(s) 319, 1148, 1919, 1982, 2223
MboII GAAGA 8 cut(s) 982, 1025, 1140, 1143, 1391, 1505, 1907, 2134
MfeI CAATTG 3 cut(s) 54, 708, 2219
MflI RGATCY 2 cut(s) 1148, 1919
MhlI GDGCHC 3 cut(s) 191, 626, 1206
MlsI TGGCCA 1 cut(s) 2373
MluNI TGGCCA 1 cut(s) 2373
MlyI GAGTC 2 cut(s) 43, 119
MmeI TCCRAC 3 cut(s) 1145, 1511, 2086
Mox20I TGGCCA 1 cut(s) 2373
Mph1103I ATGCAT 1 cut(s) 1778
MroXI GAANNNNTTC 3 cut(s) 401, 1224, 2023
MscI TGGCCA 1 cut(s) 2373
MseI TTAA 8 cut(s) 390, 747, 984, 1118, 1344, 1367, 1629, 2010
MslI CAYNNNNRTG 4 cut(s) 629, 945, 1044, 2280
Msp20I TGGCCA 1 cut(s) 2373
MspR9I CCNGG 4 cut(s) 1200, 1828, 1961, 2135
MunI CAATTG 3 cut(s) 54, 708, 2219
MvaI CCWGG 4 cut(s) 1200, 1828, 1961, 2135
MwoI GCNNNNNNNGC 3 cut(s) 994, 1265, 1442
NcoI CCATGG 2 cut(s) 98, 1754
NdeI CATATG 2 cut(s) 2156, 2239
NdeII GATC 5 cut(s) 319, 1148, 1919, 1982, 2223
NlaIII CATG 7 cut(s) 102, 1597, 1604, 1758, 1780, 1785, 1918
NlaIV GGNNCC 4 cut(s) 793, 1921, 2026, 2295
NmuCI GTSAC 4 cut(s) 115, 947, 2084, 2190
NsiI ATGCAT 1 cut(s) 1778
NspI RCATGY 2 cut(s) 1597, 1785
OliI CACNNNNGTG 1 cut(s) 629
PaqCI CACCTGC 2 cut(s) 28, 1785
PceI AGGCCT 1 cut(s) 1352
PciI ACATGT 2 cut(s) 1593, 1781
PciSI GCTCTTC 1 cut(s) 1518
PdmI GAANNNNTTC 3 cut(s) 401, 1224, 2023
PflMI CCANNNNNTGG 3 cut(s) 275, 1745, 2034
PfoI TCCNGGA 1 cut(s) 2133
PkrI GCNGC 8 cut(s) 138, 510, 999, 1060, 1432, 1435, 1852, 2074
PleI GAGTC 2 cut(s) 43, 118
PpsI GAGTC 2 cut(s) 43, 118
PpuMI RGGWCCY 1 cut(s) 597
PscI ACATGT 2 cut(s) 1593, 1781
PsiI TTATAA 3 cut(s) 1112, 1214, 2268
Psp5II RGGWCCY 1 cut(s) 597
Psp6I CCWGG 4 cut(s) 1198, 1826, 1959, 2133
PspGI CCWGG 4 cut(s) 1198, 1826, 1959, 2133
PspN4I GGNNCC 4 cut(s) 793, 1921, 2026, 2295
PspPI GGNCC 2 cut(s) 597, 791
PspPPI RGGWCCY 1 cut(s) 597
PstI CTGCAG 1 cut(s) 426
PstNI CAGNNNCTG 1 cut(s) 866
PsuI RGATCY 2 cut(s) 1148, 1919
RsaI GTAC 4 cut(s) 179, 646, 762, 1732
RsaNI GTAC 4 cut(s) 178, 645, 761, 1731
RseI CAYNNNNRTG 4 cut(s) 629, 945, 1044, 2280
SalI GTCGAC 2 cut(s) 4, 495
SapI GCTCTTC 1 cut(s) 1518
SaqAI TTAA 8 cut(s) 390, 747, 984, 1118, 1344, 1367, 1629, 2010
SatI GCNGC 8 cut(s) 137, 509, 998, 1059, 1431, 1434, 1851, 2073
Sau3AI GATC 5 cut(s) 319, 1148, 1919, 1982, 2223
Sau96I GGNCC 2 cut(s) 597, 791
ScaI AGTACT 1 cut(s) 1732
SchI GAGTC 2 cut(s) 43, 119
ScrFI CCNGG 4 cut(s) 1200, 1828, 1961, 2135
SduI GDGCHC 3 cut(s) 191, 626, 1206
SfaNI GCATC 4 cut(s) 243, 1045, 1943, 2099
SfcI CTRYAG 2 cut(s) 422, 720
SinI GGWCC 2 cut(s) 597, 791
SmiMI CAYNNNNRTG 4 cut(s) 629, 945, 1044, 2280
SpeI ACTAGT 2 cut(s) 1238, 1733
SseBI AGGCCT 1 cut(s) 1352
SsiI CCGC 4 cut(s) 543, 945, 976, 1850
SspI AATATT 1 cut(s) 1143
SspMI CTAG 7 cut(s) 1239, 1517, 1620, 1656, 1734, 1854, 2261
StuI AGGCCT 1 cut(s) 1352
StyD4I CCNGG 4 cut(s) 1198, 1826, 1959, 2133
StyI CCWWGG 3 cut(s) 98, 593, 1754
TaaI ACNGT 6 cut(s) 121, 847, 1641, 2210, 2246, 2398
TaiI ACGT 1 cut(s) 2059
TaqI TCGA 4 cut(s) 5, 191, 414, 496
TatI WGTACW 4 cut(s) 177, 644, 760, 1730
TauI GCSGC 1 cut(s) 1853
Tru1I TTAA 8 cut(s) 390, 747, 984, 1118, 1344, 1367, 1629, 2010
Tru9I TTAA 8 cut(s) 390, 747, 984, 1118, 1344, 1367, 1629, 2010
TscAI CASTG 5 cut(s) 126, 468, 528, 850, 1191
TseFI GTSAC 4 cut(s) 115, 947, 2084, 2190
TseI GCWGC 7 cut(s) 136, 508, 997, 1058, 1430, 1433, 2072
Tsp45I GTSAC 4 cut(s) 115, 947, 2084, 2190
TspDTI ATGAA 9 cut(s) 783, 929, 1017, 1152, 1556, 1586, 1589, 1680, 2354
TspGWI ACGGA 1 cut(s) 641
TspRI CASTG 5 cut(s) 126, 468, 528, 850, 1191
Van91I CCANNNNNTGG 3 cut(s) 275, 1745, 2034
VpaK11BI GGWCC 2 cut(s) 597, 791
XapI RAATTY 4 cut(s) 69, 332, 526, 777
XbaI TCTAGA 1 cut(s) 1516
XceI RCATGY 2 cut(s) 1597, 1785
XcmI CCANNNNNNNNNTGG 1 cut(s) 1304
XmiI GTMKAC 4 cut(s) 5, 496, 715, 1235
XmnI GAANNNNTTC 3 cut(s) 401, 1224, 2023
XspI CTAG 7 cut(s) 1239, 1517, 1620, 1656, 1734, 1854, 2261
ZrmI AGTACT 1 cut(s) 1732
Zsp2I ATGCAT 1 cut(s) 1778
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.