Rorug01G0131200

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001590
Physical Location & Seq
Reverse (-)
22263203 .. 22264901
1699 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0131200.1

Sequence Viewer

Length: 1485 bp
ATGGTTCATTTCAACCAAGCTTTGCTCGCGAAGCAAGGTTGGAGAATTCTTCAGTCACCGGATTCATTGGTAGCTAGGTTGTACAAAGCTCGGTATTTCCCGCGGTGTGACTTTCTGGATGCTGTAGTGCCAAAAGGGGCCTCGTATGCATGGCGTAGTATCATGCATGGCAAAAATCTGTTACGTAGAGGGGTGAGATATAGGGTTGGCAATGGAGCACATATTTCTGCATGGCGGGACCCGTGGGTTCCACTACCCATACATTTCAAACCATTTTCCTCTCCAGCTGTGGGCTATGAAGAATTGAGAGTATGTGACTTAATGGTGGAGAACGATTATGAGTGGAATGTTCCACTTTTGGAAGCTTTATTTACACCACTGGAAGTTGGCATAATTGCAAGTATCCCTCTAAGCCTTAGAGGAGCAAATGACAGCTTGATATGGCACTATGATAAGAGAGGAAACTATGGAGTGAAAAGTGGATATCATTTGGCCCGCCTTGATGATGGTAGATCTGACCGGGCTTCTAGTTCAAATGGGTCTTATGGGATTAATGCTACTTACTGGAAGATAATTTGGGGGGCCAACATCCCACCTAAAGTGCGTGTGTTCGTATGGAGACTACTCAGAGGCATTTTACCTACAAGAAGAGCTCTCTCCCATAAGGTGGAGGCTTCATGTGTGGAGGATTGGGTGTTGAACGTCATTGATAAATTGGATGGTAACCAACGTTGTGCTTTTTTCATGGCACTCTGGGTCATATGGTCTGAAAGAAATAATGTCTTGTGGAACGGAAATTTCTTCTGTGCTTCTAATGCTGCTCAATGGGCTAGTAAATTTCTTGAGGAATATCAGCAACTTCATGTGCATGGAAATGCGAAAGGTAGAAGGGAAAAGTCCAAATGGCAGAACCCCCCTAGTGGGCGTCTGAAAGTGAATGTGGATGGGAGTTACCGGGCTGATCATGGTGATGGTGGAGTGGGAGTTGTGATACGTGATGAAAATGGTATGTGTTTGTCAGCTCTGGCTCGATATTTCCCACATGCTTCATCTGCTCTACATATGGAGGTTGAAGCTTGTAGGGCTGGGCTTTTATTGGCAATCCATCAGGACATGACCGTTATTGATATTGAGAGCGATTGTTCCCTTGCTGTCACTGCTATACAGCGTGAGGTGGAAGATAGATCTGACATTGGGCGAATTATCGATGATTGTAAGTCATATTTGACATCTTTTCACTCAAGTCAGGTTAGCCACATCTTCCGTGAAGCAAACGGTGTGGCCAACAGGTTAGCGCACCTTGCTAGTTTTAATTATATTTCTGATGTTTGGTTAGATGAGACTCCTGTTATTATTCGGGATGTTCTCTATGAGGATGTTTGTAATCTTTCTAATGTCGCTCGGGGTTCAGGTATTATGTCCCCCCCGTTGCAAAGTTTTAATTCAATAAATGGAATCGGGCGTGGGGCTGAGCCTCCCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C PP-binding PQQ PQQ_2 PQQ_3

Protein Analysis

494

Amino Acids

55.55

Weight (kDa)

8.44

Isoelectric Point (pI)

37.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 186 - 221 9.6e-06 zinc-binding in reverse transcriptase
RVT_3 PF13456 313 - 434 2.2e-25 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 667
AccII CGCG 2 cut(s) 29, 103
AciI CCGC 4 cut(s) 101, 103, 235, 496
AclI AACGTT 1 cut(s) 730
AcoI YGGCCR 1 cut(s) 1281
AcsI RAATTY 3 cut(s) 45, 796, 836
AcuI CTGAAG 1 cut(s) 35
AcyI GRCGYC 1 cut(s) 925
AfaI GTAC 1 cut(s) 83
AfiI CCNNNNNNNGG 4 cut(s) 290, 667, 920, 921
AgsI TTSAA 6 cut(s) 13, 268, 534, 700, 1073, 1446
Alw21I GWGCWC 2 cut(s) 220, 655
Alw26I GTCTC 2 cut(s) 613, 1334
Ama87I CYCGRG 1 cut(s) 1401
AoxI GGCC 4 cut(s) 138, 492, 582, 1281
ApeKI GCWGC 1 cut(s) 818
ApoI RAATTY 3 cut(s) 45, 796, 836
AseI ATTAAT 1 cut(s) 552
AspLEI GCGC 1 cut(s) 1297
AspS9I GGNCC 4 cut(s) 138, 238, 493, 582
AsuC2I CCSGG 2 cut(s) 521, 956
AsuHPI GGTGA 3 cut(s) 48, 205, 980
AvaI CYCGRG 1 cut(s) 1401
AvaII GGWCC 1 cut(s) 238
BalI TGGCCA 1 cut(s) 1283
BanII GRGCYC 1 cut(s) 655
Bbv12I GWGCWC 2 cut(s) 220, 655
BbvI GCAGC 1 cut(s) 805
BccI CCATC 5 cut(s) 500, 713, 938, 965, 1113
BciVI GTATCC 1 cut(s) 413
BclI TGATCA 1 cut(s) 961
BcnI CCSGG 2 cut(s) 521, 956
BcoDI GTCTC 2 cut(s) 613, 1334
BfaI CTAG 6 cut(s) 75, 528, 831, 918, 1305, 1483
BfmI CTRYAG 1 cut(s) 123
BfuI GTATCC 1 cut(s) 413
BglII AGATCT 2 cut(s) 512, 1184
BisI GCNGC 1 cut(s) 819
BlpI GCTNAGC 1 cut(s) 1470
BlsI GCNGC 1 cut(s) 820
Bme1390I CCNGG 2 cut(s) 521, 956
Bme18I GGWCC 1 cut(s) 238
BmeT110I CYCGRG 1 cut(s) 1401
BmgT120I GGNCC 4 cut(s) 138, 238, 493, 582
BmiI GGNNCC 5 cut(s) 139, 239, 240, 249, 583
BmrFI CCNGG 2 cut(s) 521, 956
BmsI GCATC 1 cut(s) 109
BpmI CTGGAG 1 cut(s) 267
Bpu1102I GCTNAGC 1 cut(s) 1470
BpuEI CTTGAG 2 cut(s) 863, 1225
BpuMI CCSGG 2 cut(s) 521, 956
Bsa29I ATCGAT 1 cut(s) 1206
BsaAI YACGTR 2 cut(s) 185, 995
BsaHI GRCGYC 1 cut(s) 925
BsaJI CCNNGG 2 cut(s) 101, 242
BsaWI WCCGGW 1 cut(s) 58
BsaXI ACNNNNNCTCC 2 cut(s) 969, 999
Bsc4I CCNNNNNNNGG 4 cut(s) 290, 667, 920, 921
Bse1I ACTGG 2 cut(s) 384, 569
Bse3DI GCAATG 1 cut(s) 217
BseCI ATCGAT 1 cut(s) 1206
BseDI CCNNGG 2 cut(s) 101, 242
BseGI GGATG 6 cut(s) 124, 588, 724, 949, 1366, 1381
BseLI CCNNNNNNNGG 4 cut(s) 290, 667, 920, 921
BseMI GCAATG 1 cut(s) 217
BseMII CTCAG 2 cut(s) 640, 1461
BseNI ACTGG 2 cut(s) 384, 569
BseRI GAGGAG 1 cut(s) 435
BseXI GCAGC 1 cut(s) 805
BseYI CCCAGC 2 cut(s) 1085, 1478
Bsh1236I CGCG 2 cut(s) 29, 103
BshFI GGCC 4 cut(s) 140, 494, 584, 1283
BshVI ATCGAT 1 cut(s) 1206
BsiHKAI GWGCWC 2 cut(s) 220, 655
BsiHKCI CYCGRG 1 cut(s) 1401
BsiSI CCGG 3 cut(s) 59, 520, 955
BslFI GGGAC 2 cut(s) 251, 1405
BslI CCNNNNNNNGG 4 cut(s) 290, 667, 920, 921
BsmAI GTCTC 2 cut(s) 613, 1334
BsmFI GGGAC 2 cut(s) 251, 1405
BsnI GGCC 4 cut(s) 140, 494, 584, 1283
BsoBI CYCGRG 1 cut(s) 1401
Bsp1286I GDGCHC 2 cut(s) 220, 655
Bsp1407I TGTACA 1 cut(s) 81
Bsp143I GATC 3 cut(s) 512, 961, 1184
Bsp1720I GCTNAGC 1 cut(s) 1470
Bsp68I TCGCGA 1 cut(s) 29
BspACI CCGC 4 cut(s) 101, 103, 235, 496
BspANI GGCC 4 cut(s) 140, 494, 584, 1283
BspCNI CTCAG 2 cut(s) 639, 1462
BspDI ATCGAT 1 cut(s) 1206
BspFNI CGCG 2 cut(s) 29, 103
BspLI GGNNCC 5 cut(s) 139, 239, 240, 249, 583
BspQI GCTCTTC 1 cut(s) 643
BsrDI GCAATG 1 cut(s) 217
BsrGI TGTACA 1 cut(s) 81
BsrI ACTGG 2 cut(s) 384, 569
BssECI CCNNGG 2 cut(s) 101, 242
BssMI GATC 3 cut(s) 512, 961, 1184
BssNI GRCGYC 1 cut(s) 925
Bst4CI ACNGT 2 cut(s) 1120, 1277
Bst6I CTCTTC 1 cut(s) 643
BstACI GRCGYC 1 cut(s) 925
BstAUI TGTACA 1 cut(s) 81
BstBAI YACGTR 2 cut(s) 185, 995
BstC8I GCNNGC 2 cut(s) 27, 496
BstDEI CTNAG 4 cut(s) 410, 416, 626, 1470
BstDSI CCRYGG 2 cut(s) 101, 242
BstEII GGTNACC 1 cut(s) 722
BstF5I GGATG 6 cut(s) 124, 588, 724, 949, 1366, 1381
BstFNI CGCG 2 cut(s) 29, 103
BstHHI GCGC 1 cut(s) 1297
BstKTI GATC 3 cut(s) 515, 964, 1187
BstMAI GTCTC 2 cut(s) 613, 1334
BstMBI GATC 3 cut(s) 512, 961, 1184
BstMWI GCNNNNNNNGC 9 cut(s) 26, 31, 146, 815, 827, 1052, 1082, 1157, 1301
BstNSI RCATGY 1 cut(s) 1046
BstPI GGTNACC 1 cut(s) 722
BstSCI CCNGG 2 cut(s) 519, 954
BstSFI CTRYAG 1 cut(s) 123
BstSNI TACGTA 1 cut(s) 185
BstUI CGCG 2 cut(s) 29, 103
BstV1I GCAGC 1 cut(s) 805
BstX2I RGATCY 2 cut(s) 512, 1184
BstYI RGATCY 2 cut(s) 512, 1184
Bsu15I ATCGAT 1 cut(s) 1206
BsuI GTATCC 1 cut(s) 413
BsuRI GGCC 4 cut(s) 140, 494, 584, 1283
BsuTUI ATCGAT 1 cut(s) 1206
BtgI CCRYGG 2 cut(s) 101, 242
BtsCI GGATG 6 cut(s) 124, 588, 724, 949, 1366, 1381
BtsI GCAGTG 1 cut(s) 1155
BtsIMutI CAGTG 2 cut(s) 377, 1155
BtuMI TCGCGA 1 cut(s) 29
Cac8I GCNNGC 2 cut(s) 27, 496
CfoI GCGC 1 cut(s) 1297
Cfr13I GGNCC 4 cut(s) 138, 238, 493, 582
Cfr42I CCGCGG 1 cut(s) 104
ClaI ATCGAT 1 cut(s) 1206
CseI GACGC 1 cut(s) 914
Csp6I GTAC 1 cut(s) 82
CspCI CAANNNNNGTGG 2 cut(s) 1260, 1295
CviQI GTAC 1 cut(s) 82
DdeI CTNAG 4 cut(s) 410, 416, 626, 1470
DpnI GATC 3 cut(s) 514, 963, 1186
DpnII GATC 3 cut(s) 512, 961, 1184
EaeI YGGCCR 1 cut(s) 1281
Eam1104I CTCTTC 1 cut(s) 643
EarI CTCTTC 1 cut(s) 643
Ecl136II GAGCTC 1 cut(s) 653
Eco105I TACGTA 1 cut(s) 185
Eco24I GRGCYC 1 cut(s) 655
Eco32I GATATC 1 cut(s) 485
Eco47I GGWCC 1 cut(s) 238
Eco53kI GAGCTC 1 cut(s) 653
Eco57I CTGAAG 1 cut(s) 35
Eco88I CYCGRG 1 cut(s) 1401
Eco91I GGTNACC 1 cut(s) 722
EcoICRI GAGCTC 1 cut(s) 653
EcoO109I RGGNCCY 2 cut(s) 138, 238
EcoO65I GGTNACC 1 cut(s) 722
EcoRI GAATTC 1 cut(s) 45
EcoRV GATATC 1 cut(s) 485
EcoT22I ATGCAT 2 cut(s) 151, 168
EcoT38I GRGCYC 1 cut(s) 655
FaqI GGGAC 2 cut(s) 251, 1405
FauI CCCGC 3 cut(s) 108, 228, 503
FauNDI CATATG 2 cut(s) 761, 1062
FbaI TGATCA 1 cut(s) 961
Fnu4HI GCNGC 1 cut(s) 819
FokI GGATG 6 cut(s) 131, 575, 731, 956, 1373, 1388
FriOI GRGCYC 1 cut(s) 655
Fsp4HI GCNGC 1 cut(s) 819
FspBI CTAG 6 cut(s) 75, 528, 831, 918, 1305, 1483
GlaI GCGC 1 cut(s) 1296
GluI GCNGC 1 cut(s) 819
GsaI CCCAGC 2 cut(s) 1089, 1482
GsuI CTGGAG 1 cut(s) 267
HaeIII GGCC 4 cut(s) 140, 494, 584, 1283
HapII CCGG 3 cut(s) 59, 520, 955
HgaI GACGC 1 cut(s) 914
HhaI GCGC 1 cut(s) 1297
Hin1I GRCGYC 1 cut(s) 925
Hin6I GCGC 1 cut(s) 1295
HinP1I GCGC 1 cut(s) 1295
HindIII AAGCTT 3 cut(s) 18, 363, 1074
HinfI GANTC 3 cut(s) 62, 1342, 1455
HpaII CCGG 3 cut(s) 59, 520, 955
HphI GGTGA 3 cut(s) 48, 205, 980
Hpy188I TCNGA 6 cut(s) 517, 629, 769, 930, 1189, 1324
Hpy188III TCNNGA 5 cut(s) 28, 116, 842, 1109, 1358
HpyAV CCTTC 1 cut(s) 882
HpyCH4III ACNGT 2 cut(s) 1120, 1277
HpyCH4IV ACGT 4 cut(s) 184, 702, 730, 994
HpyCH4V TGCA 6 cut(s) 149, 166, 230, 398, 868, 1432
HpyF10VI GCNNNNNNNGC 9 cut(s) 26, 31, 146, 815, 827, 1052, 1082, 1157, 1301
HpyF3I CTNAG 4 cut(s) 410, 416, 626, 1470
HpySE526I ACGT 4 cut(s) 184, 702, 730, 994
Hsp92I GRCGYC 1 cut(s) 925
HspAI GCGC 1 cut(s) 1295
KflI GGGWCCC 1 cut(s) 238
Ksp22I TGATCA 1 cut(s) 961
KspI CCGCGG 1 cut(s) 104
Kzo9I GATC 3 cut(s) 512, 961, 1184
LguI GCTCTTC 1 cut(s) 643
LmnI GCTCC 2 cut(s) 215, 422
Lsp1109I GCAGC 1 cut(s) 805
LweI GCATC 1 cut(s) 109
MaeI CTAG 6 cut(s) 75, 528, 831, 918, 1305, 1483
MaeII ACGT 4 cut(s) 184, 702, 730, 994
MaeIII GTNAC 7 cut(s) 54, 107, 180, 314, 722, 950, 1153
MalI GATC 3 cut(s) 514, 963, 1186
MboI GATC 3 cut(s) 512, 961, 1184
MboII GAAGA 7 cut(s) 41, 311, 580, 660, 793, 1190, 1252
MflI RGATCY 2 cut(s) 512, 1184
MhlI GDGCHC 2 cut(s) 220, 655
MlsI TGGCCA 1 cut(s) 1283
MluNI TGGCCA 1 cut(s) 1283
MlyI GAGTC 1 cut(s) 1336
MmeI TCCRAC 1 cut(s) 20
Mox20I TGGCCA 1 cut(s) 1283
Mph1103I ATGCAT 2 cut(s) 151, 168
MscI TGGCCA 1 cut(s) 1283
MseI TTAA 4 cut(s) 320, 552, 1311, 1440
MslI CAYNNNNRTG 3 cut(s) 867, 873, 969
Msp20I TGGCCA 1 cut(s) 1283
MspA1I CMGCKG 2 cut(s) 103, 287
MspI CCGG 3 cut(s) 59, 520, 955
MspR9I CCNGG 2 cut(s) 521, 956
MvnI CGCG 2 cut(s) 29, 103
MwoI GCNNNNNNNGC 9 cut(s) 26, 31, 146, 815, 827, 1052, 1082, 1157, 1301
NciI CCSGG 2 cut(s) 521, 956
NdeI CATATG 2 cut(s) 761, 1062
NdeII GATC 3 cut(s) 512, 961, 1184
NlaIV GGNNCC 5 cut(s) 139, 239, 240, 249, 583
NmuCI GTSAC 4 cut(s) 54, 107, 314, 1153
NruI TCGCGA 1 cut(s) 29
NsiI ATGCAT 2 cut(s) 151, 168
NspI RCATGY 1 cut(s) 1046
PciSI GCTCTTC 1 cut(s) 643
PfeI GAWTC 2 cut(s) 62, 1455
PflMI CCANNNNNTGG 1 cut(s) 667
PkrI GCNGC 1 cut(s) 820
PleI GAGTC 1 cut(s) 1336
PpsI GAGTC 1 cut(s) 1336
Ppu21I YACGTR 2 cut(s) 185, 995
PpuMI RGGWCCY 1 cut(s) 238
PshBI ATTAAT 1 cut(s) 552
Psp124BI GAGCTC 1 cut(s) 655
Psp1406I AACGTT 1 cut(s) 730
Psp5II RGGWCCY 1 cut(s) 238
PspEI GGTNACC 1 cut(s) 722
PspFI CCCAGC 2 cut(s) 1085, 1478
PspN4I GGNNCC 5 cut(s) 139, 239, 240, 249, 583
PspPI GGNCC 4 cut(s) 138, 238, 493, 582
PspPPI RGGWCCY 1 cut(s) 238
PsuI RGATCY 2 cut(s) 512, 1184
PvuII CAGCTG 1 cut(s) 287
RruI TCGCGA 1 cut(s) 29
RsaI GTAC 1 cut(s) 83
RsaNI GTAC 1 cut(s) 82
RseI CAYNNNNRTG 3 cut(s) 867, 873, 969
SacI GAGCTC 1 cut(s) 655
SacII CCGCGG 1 cut(s) 104
SapI GCTCTTC 1 cut(s) 643
SaqAI TTAA 4 cut(s) 320, 552, 1311, 1440
SatI GCNGC 1 cut(s) 819
Sau3AI GATC 3 cut(s) 512, 961, 1184
Sau96I GGNCC 4 cut(s) 138, 238, 493, 582
SchI GAGTC 1 cut(s) 1336
ScrFI CCNGG 2 cut(s) 521, 956
SduI GDGCHC 2 cut(s) 220, 655
SfaNI GCATC 1 cut(s) 109
SfcI CTRYAG 1 cut(s) 123
Sfr303I CCGCGG 1 cut(s) 104
SgrBI CCGCGG 1 cut(s) 104
SinI GGWCC 1 cut(s) 238
SmiMI CAYNNNNRTG 3 cut(s) 867, 873, 969
SmlI CTYRAG 2 cut(s) 842, 1240
SmoI CTYRAG 2 cut(s) 842, 1240
SnaBI TACGTA 1 cut(s) 185
SsiI CCGC 4 cut(s) 101, 103, 235, 496
SspMI CTAG 6 cut(s) 75, 528, 831, 918, 1305, 1483
SstI GAGCTC 1 cut(s) 655
StyD4I CCNGG 2 cut(s) 519, 954
TaaI ACNGT 2 cut(s) 1120, 1277
TaiI ACGT 4 cut(s) 187, 705, 733, 997
TaqI TCGA 2 cut(s) 1030, 1206
TatI WGTACW 1 cut(s) 81
TfiI GAWTC 2 cut(s) 62, 1455
Tru1I TTAA 4 cut(s) 320, 552, 1311, 1440
Tru9I TTAA 4 cut(s) 320, 552, 1311, 1440
TscAI CASTG 2 cut(s) 384, 1162
TseFI GTSAC 4 cut(s) 54, 107, 314, 1153
TseI GCWGC 1 cut(s) 818
Tsp45I GTSAC 4 cut(s) 54, 107, 314, 1153
TspDTI ATGAA 7 cut(s) 54, 312, 666, 733, 851, 1014, 1038
TspGWI ACGGA 2 cut(s) 807, 1253
TspRI CASTG 2 cut(s) 384, 1162
Van91I CCANNNNNTGG 1 cut(s) 667
VpaK11BI GGWCC 1 cut(s) 238
VspI ATTAAT 1 cut(s) 552
XapI RAATTY 3 cut(s) 45, 796, 836
XceI RCATGY 1 cut(s) 1046
XspI CTAG 6 cut(s) 75, 528, 831, 918, 1305, 1483
Zsp2I ATGCAT 2 cut(s) 151, 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.